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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean Journal of Agricultural Science
Journal Basic Information
Journal DOI :
CNU Journal of Agricultural Science
Editor in Chief :
Volume & Issues
Volume 42, Issue 4 - Dec 2015
Volume 42, Issue 3 - Sep 2015
Volume 42, Issue 2 - Jun 2015
Volume 42, Issue 1 - Mar 2015
Selecting the target year
Gene expression in plant according to RNAi treatment of the tobacco whitefly
Kim, Jeong-Hee ; Seo, Eun-Young ; Kim, Jung-Kyu ; Lim, Hyoun-Sub ; Yu, Yong-Man ; Youn, Young-Nam ;
Korean Journal of Agricultural Science, volume 42, issue 2, 2015, Pages 81~86
DOI : 10.7744/cnujas.2015.42.2.081
Three genes selected from cDNA library of tobacco whitefly, Bemisia tabaci, were checked whether these genes expressed in plant or not, and confirmed the change of gene expression using qRT-PCR in the tobacco whitefly. First of all, three genes were inserted in Tobacco rattle virus (TRV) RNA2 vector using Sac I and Xho I restriction enzymes, and conducted agro-infiltration in tobacco plants (Nicotiana benthamianana). And then, it was confirmed that TRV RNA2 vector and genes inserted in TRV RNA2 vector were expressed in plant. So, after feeding the tobacco whitefly the plants inoculated the genes and induced RNAi of the genes, we plan to confirm the RNAi in the whitefly and investigate the changes of gene expression through the qRT-PCR.
Herbicidal efficacy of carfentrazone-ethyl mixtures in direct-seeding flooded rice
Won, OK Jae ; Park, Kee Woong ; Park, Su Hyuk ; Eom, Min Yong ; Hwang, Ki Seon ; Kim, Young Tae ; Pyon, Jong Yeong ;
Korean Journal of Agricultural Science, volume 42, issue 2, 2015, Pages 87~92
DOI : 10.7744/cnujas.2015.42.2.087
This study was conducted to evaluate the herbicidal efficacy of carfentrazone-ethyl mixtures in direct-seeding flooded rice. The efficacy of three different carfentrazone-ethly mixtures was greater than pyrazosulfuron-ethyl pyriminobac-methyl GR (PP GR). Weeding efficacy of three different carfentrazone-ethly mixtures was more than 96.9%. No phytotoxic effect was observed in the rice based on the plant height and the number of tillers. Yield of rice un three different carfentrazone-ethly mixtures application was more than both in the PP GR and in the hand weeding. Based on these data, three different carfentrazone-ethly mixtures can be applied to provide an effective weed management in direct-seeding flooded rice.
Weed control as affected by herbicide in winter cereal crops
Won, Ok Jae ; Park, Kee Woong ; Park, Su Hyuk ; Eom, Min Yong ; Kang, Kwang Sik ;
Korean Journal of Agricultural Science, volume 42, issue 2, 2015, Pages 93~98
DOI : 10.7744/cnujas.2015.42.2.093
This study was conducted to evaluate the efficacy of herbicide in winter cereal crops, barley and wheat. The efficacy of soil-applied herbicides, linuron EC and pendimethalin, thiobencarb GR was greater than that of butachlor EC. Linuron EC controlled average 95% and pendimethalin, thiobencarb GR controlled average 97% within 150 days after soil-applied treatment. When compared with untreated control, no visual injuries were detected at single and double dosage of linuron EC and pendimethalin, thiobencarb GR. The yield of barley increased in linuron EC and wheat increased in pendimethalin, thiobencarb GR treatments when compared with butachlor EC. The efficacy of foliage treatment, bentazone+propanil ME was greater than that of bentazone SL. Bentazone+propanil ME controlled average 96% in barley and 97% in wheat, within 30 days after foliage treatment. When compared with untreated control, no visual injuries were detected at single and double dosage of bentazone+propanil ME. The yield of barley and wheat increased in bentazone+propanil ME treatments when compared with bentazone SL. Based on these data, combinations of these herbicides could be applied to provide effective weed management in barley and wheat field.
Development of a diagnostic system to detect potato virus T using RT-PCR and nested PCR
Lee, Si Won ; Shin, Yong-Gil ; Lee, Jin-Young ; Kim, Young-Suk ; Yang, Mi Hee ; Choi, In-Cheol ;
Korean Journal of Agricultural Science, volume 42, issue 2, 2015, Pages 99~103
DOI : 10.7744/cnujas.2015.42.2.099
Potato virus T (PVT) is a plant pathogen in the family Betaflexiviridae, group IV single-stranded positive sense RNA viruses. The major host of PVT is potato, and it has been reported in Ullucus tuberosus, Oxalis tuberosa and Tropaeolum tuberosum. This study aimed at developing reverse transcription (RT)-polymerase chain reaction (PCR) and nested PCR techniques for specific detection of PVT. Finally, Two RT-PCR primer sets were developed and verified. The RT-PCR products were amplified to 734 (PVT RT-PCR primer set 6) and 828 bp (PVT RT-PCR primer set 29) long to detect PVT. The nested PCR primer sets [PVT-N70/C20 (
) and PVT-N75/C30 (
)] were developed which are high sensitivity and verification for detection of PVT. Furthermore, a modified-positive control plasmid is use to verify contamination of laboratory in PVT detection. This study supported the diagnose PVT in potato or PVT related hosts.
Development of PCR-base Diagnostic System for the Detection of Andean potato latent virus
Lee, Jin-Young ; Kim, Jin-Ho ; Kim, Eunsil ; Lee, Siwon ;
Korean Journal of Agricultural Science, volume 42, issue 2, 2015, Pages 105~109
DOI : 10.7744/cnujas.2015.42.2.105
Andean potato latent virus (APLV) is a phytopathogenic virus that belongs to the Group IV (+) sense ssRNA viruses of the genus Tymovirus. It mainly infects potatoes and is specified as a controlled quarantine virus in Korea. In this study, two primer sets of RT-PCR and nested PCR [set 2 (
) and set 23 (
)], were selected, which can rapidly and accurately diagnose APLV in quarantine sites. In addition, a modified-positive control plasmid is development, can possible verification of laboratory contamination in diagnosis of APLV detection. The PCR-base system developed in this study is expected to diagnose APLV and contribute to the plant quarantine in Korea.
Effect of rice bran and its mixture with pine leaves on efficacy of weed control and growth and yield of rice in paddy fields
Lee, Sang Ill ; Park, Kee Woong ; Won, OK Jae ; Park, Su Hyuk ; Eom, Min Yong ; Hwang, Ki Seon ; Kim, Young Tae ; Pyon, Jong Yeong ;
Korean Journal of Agricultural Science, volume 42, issue 2, 2015, Pages 111~116
DOI : 10.7744/cnujas.2015.42.2.111
Combined applications of rice bran with pine leaves were tested to examine the inhibitory effects to paddy weeds and increased yield of rice for developing techniques of environment-friendly weed management in paddy rice fields. Weed control efficacy at 60 days after treatment was improved to 88.8% by combined application of rice bran with pine leaves, while weed control efficacy by single application of rice bran showed 67.5%. The other weed control efficacy combined with chestnut leaves and barley straws were 76.3% and 69.9% respectively. Combined application of rice bran with pine leaves was more effective to broadleaf weeds such as Monochoria vaginalis. Weed control efficacy by combined application with pine leaves was 100% until 70 days after rice transplanting and maintained weed control effect up to 90 days after transplanting. Rice yield by combined application of rice bran with pine leaves was 526 kg/10 a, which was higher yield than by combined application with barley straws, single application of rice bran, natural snail application and hand weeding.
Analysis of working posture of forest trail construction
Lee, Myeong-Kyo ; Park, Bum-Jin ; Lee, Joon-Woo ; Choi, Sung-Min ;
Korean Journal of Agricultural Science, volume 42, issue 2, 2015, Pages 117~124
DOI : 10.7744/cnujas.2015.42.2.117
In forest work, working conditions are very hard to improve. The good posture is believed to bring about direct improvements such as accident prevention. Therefore, this research carried on analysis of working posture in forest work (construct in stepping-stone) using OWAS analysis system. According to the analytical results provided by OWAS, the ratio of category III (Work posture has a distinctly harmful effect on the musculoskeletal system) has shawn that worker 2 was 32.2%, worker 1 was 25.2% and worker 3 was 15.5%. Furthermore, the ratio of category IV (Work posture with an extremely harmful effect on the musculoskeletal system) has shown that worker 2 was 9.8%, worker 3 was 1.4% and worker 1 was 1.2%. According to the OWAS method, percentage of OWAS action categories III and IV in the worker 2 was higher than another workers.
Influence of bone fracture incidence on the quality of pork semimembranous muscle
Jung, Samooel ; Lee, Hanhyeon ; Hwang, Heetae ; Lim, Daewoon ; Lee, Chulwoo ; Jo, Cheorun ;
Korean Journal of Agricultural Science, volume 42, issue 2, 2015, Pages 125~129
DOI : 10.7744/cnujas.2015.42.2.125
This study was conducted to investigate the incidence of bone-fracture from pig carcass slaughtered in eight processing plants located in Daejeon and Chungnam area, Korea, during the year 2013 and 2014, and to evaluate the quality of semimembranous muscle from bone-fractured carcasses. Twenty semimembranous muscles were collected from bone-fractured carcasses and none bone-fractured (control) ones, respectively, after storage of pig carcass at
for 24 h. The pH, cooking loss, and color of semimembranous muscle were measured as quality parameters. In total 4,865,502 of pig carcasses, the occurrence of bone fracture was 0.328% (15,975 heads) and scored the highest defect (26.31%) in total abnormal carcasses. The pH and cooking loss of semimembranous muscle from bone-fractured carcasses were significantly lower than those of control (p<0.05).
values of semimembranous muscle were not significantly different between bone-fractured carcass and control whereas that of
values was significantly higher in bone-fractured carcass than control (p<0.05). Eight out of twenty semimembranous muscle collected from bone-fractured carcasses were confirmed as PSE whereas only one in control. In conclusion, the incidence of bone-fracture pre- and during slaughter of pig may cause serious defects in final meat quality. Therefore, the proper handling and treatment should be implicated to avoid and/or decrease the incidence of bone-fracture of pigs.
Quality property of the smoked breast meat produced with fresh and frozen-thawed duck meat
Lee, Hae Lim ; Koo, Bonjin ; Choi, Song-i ; Sung, Sang Hyun ; Park, Jung Hun ; Lee, Chul Woo ; Jo, Cheorun ; Jung, Samooel ;
Korean Journal of Agricultural Science, volume 42, issue 2, 2015, Pages 131~139
DOI : 10.7744/cnujas.2015.42.2.131
This study investigated the quality properties of smoked breast meats produced by fresh and frozen-thawed duck meat. Each thirty breast meats from fresh and frozen-thawed duck carcass was used for this study. The yield of smoked breast meat was measured right after curing and smoking of raw duck breast meat. And, the number of total aerobic bacteria, color, texture, and sensory property of vacuum-packaged smoked breast meats were evaluated during storage at
for 28 days. No significant difference was found in yield between smoked breast meats produced by fresh and thawed duck meats (p>0.05). The number of total aerobic bacteria and color of smoked breast meat produced by thawed duck meat were not significantly different compared with those by fresh one throughout storage period (p>0.05). The all texture properties were not significantly different between smoked breast meats produced by fresh and thawed duck meats by 14 days of storage (p>0.05). However, on day 21 and 28, the hardness and gumminess of smoked breast meat produced by fresh duck meat were significantly higher than those by thawed one (p<0.05). In sensorial property, smoked breast meat produced by thawed duck meat received significantly high scores in color, juiciness, and tenderness on days 0, 14, and 28 and in flavor and overall acceptance on days 0 and 14 compared with those by fresh one (p<0.05). Therefore, we concluded that the use of thawed duck meat for producing smoked duck meat product may be not worse than the use of fresh duck meat in quality of smoked duck meat product. In addition, the use of thawed duck meat may be better in sensorial quality of smoked duck meat product than that of fresh one.
Relationships between inbreeding coefficient and economic traits in inbred line of Duroc pigs
song, Na-Rae ; Kim, Yong-Min ; Kim, Doo-Wan ; Sa, Soo-Jin ; Kim, Ki-Hyun ; Kim, Young-Hwa ; Cho, Kyu-Ho ; Do, Chang-hee ; Hong, Joon-Ki ;
Korean Journal of Agricultural Science, volume 42, issue 2, 2015, Pages 141~149
DOI : 10.7744/cnujas.2015.42.2.141
The data of Duroc swine species that were born from 2000 to 2014 excluding missing ones collected by Korea National Institute of Animal Science were used in the present study. After removing missing data we used 9756 of productions data and 1728 of reproductive reference of breeding research to study the level of inbreeding and to investigate the impact on the reproductive traits, production traits. The correlation of reproductive traits and inbreeding coefficient are -0.07, -0.08 for total number pigs born, number of pigs born alive respectively and birth weight per litter is -0.10, number of pigs born alive per litter to 21days is -0.06 and body weight per litter to 21days is -0.09. The correlation coefficients of the inbreeding coefficients of reproductive traits are shown within 10% with negative correlation (P < 0.05). Days of 90kg and Backfat in the correlation coefficient and inbreeding coefficient production traits were not observed significant correlations, Average daily gain was investigated by the positive correlation of 0.05. According to the above results, the inbreeding level gave a negative effect on the improvement of the breed traits, investigating a relatively high compared to a negative effect on other traits. But overall correlation degree is less than 10% was observed. This inbreeding coefficient has not been clearly observed due to degeneration of the average inbreeding coefficients of these generations was maintained within 10% of the population. The scale of the experimental group was about 150 degree pig husbandry is very small compared to the advanced countries. However, the level of inbreeding in the population group with the appropriate mating combinations is maintained below 10% of population is thought to be small and can minimize the effects of inbreeding degeneration. further testing utilizing this selection is constantly considered to be necessary.