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Korean Journal of Agricultural Science
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CNU Journal of Agricultural Science
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Volume & Issues
Volume 43, Issue 2 - Jun 2016
Volume 43, Issue 1 - Mar 2016
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Perspectives on the genomics research of important crops in the tribe Andropogoneae: Focusing on the Saccharum complex
Choi, Sang Chul ; Chung, Yong Suk ; Kim, Changsoo ;
Korean Journal of Agricultural Science, volume 43, issue 1, 2016, Pages 1~13
DOI : 10.7744/kjoas.20160001
Climate changes are shifting the perception of C4 photosynthetic crops due to their superior adaptability to harsh conditions. The tribe Andropogoneae includes some economically important grasses, such as Zea mays, Sorghum bicolor, Miscanthus spp., and Saccharum spp., representing C4 photosynthetic grasses. Although the Andropogoneae grasses diverged fairly recently, their genomic structures are remarkably different from each other. As previously reported, the family Poaceae shares the pan-cereal duplication event occurring ca. 65 MYA. Since this event, Sorghum bicolor has never experienced any additional duplication event. However, some lineage-specific duplication events were reported in Z. mays and Saccharum spp., and, more recently, it was revealed that a shared allotetraploidization event occurred before the divergence between Miscanthus and Saccharum (but after the divergence from S. bicolor), which provided important clues to those two species having large genome sizes with complicated ploidy numbers. The complex genomic structures of sugarcane and Miscanthus (defined as the Saccharum complex along with some other taxa) have had a limiting effect on the use of their molecular information in breeding programs. For the last decade, genomics-associated technologies have become an important tool for molecular crop breeding (genomics-assisted breeding, GAB), but it has not been directly applied to sugarcane and Miscanthus due to their complicated genome structures. As genomics research advances, molecular breeding of those crops can take advantage of technical improvements at a reasonable cost through comparative genomic approaches. Active genomic research of non-model species using closely related model species will facilitate the improvement of those crops in the future.
Value of spray-dried plasma as a supplement to swine diets
Jang, Kibeom ; Kim, Junsu ; Kim, Sheena ; Jang, Yoontack ; Lee, Jeongjae ; Kim, Younghwa ; Park, Juncheol ; Kim, Younghoon ; Song, Minho ;
Korean Journal of Agricultural Science, volume 43, issue 1, 2016, Pages 14~20
DOI : 10.7744/kjoas.20160002
One of the most powerful health management practices is the use of antibiotics, but their use is being restricted because of health safety issues. The swine industry has been looking for various alternatives to antibiotics and increasingly considers the use of dietary factors like feed ingredients, feed additives, feed formulation practices, or feeding methods, instead of using antibiotics to improve pig health and performance. Among other alternatives to antibiotics, spray-dried plasma may be a candidate. Spray-dried plasma is a blood product that provides bioavailable nutrients and physiologically active components such as immunoglobulins, glycoproteins, growth factors, peptides, etc. It is an excellent protein source with balanced and highly digestible amino acids. Several beneficial physiological activities depend on components of spray-dried plasma, such as immune competence (antibacterial activity), modulation of microbiota and/or immune system, integrity of intestinal barrier function, etc. These beneficial effects can contribute to improvement of pig performance and health by modulation of microbiota in the digestive tract and/or immune system. Therefore, it is suggested that spray-dried plasma has great potential as an antibiotics alternative.
Occurrence of Apple stem grooving virus in commercial apple seedlings and analysis of its coat protein sequence
Han, Jae-Yeong ; Park, Chan-Hwan ; Seo, Eun-Yeong ; Kim, Jung-Kyu ; Hammond, John ; Lim, Hyoun-Sub ;
Korean Journal of Agricultural Science, volume 43, issue 1, 2016, Pages 21~27
DOI : 10.7744/kjoas.20160003
Apple stem grooving virus (ASGV), Apple chlorotic leaf spot virus (ACLSV), and Apple stem pitting virus (ASPV) have been known to induce top working disease causing economical damage in apple. Occurrences of these three viruses in pome fruit trees, including apple, have been reported around the world. The transmission of the three viruses was reported by grafting, and there was no report of transmission through mechanical contact, insect vector, or seed except some herbaceous hosts of ASGV. As RNA extraction methods for fruit trees, Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) and multiplex RT-PCR techniques have been improved for reliability and stability, and low titer viruses that could not be detected in the past have become detectable. We studied the seed transmission ability of three apple viruses through apple seedling diagnosis using RT-PCR. Nineteen seeds obtained from commercially grown apple were germinated and two of the resulting plants were ASGV positive. Seven clones of the amplified ASGV coat protein (CP) genes of these isolates were sequenced. Overall sequence identities were 99.84% (nucleotide) and 99.76% (amino acid). Presence of a previously unreported single nucleotide and amino acid variation conserved in all of these clones suggests a possible association with seed transmission of these `S` isolates. A phylogenetic tree constructed using ASGV CP nucleotide sequences showed that isolate S sequences were grouped with Korean, Chinese, Indian isolates from apple and Indian isolates from kiwi.
Growth and flowering as affected by tuber hardness, GA
concentrations and treatment duration in Calla (Zantedeschia)
Nam, Chun-Woo ; Yoo, Dong-Lim ; Kim, Su-Jeong ; Suh, Jong-Teak ; Peak, Kee Yoeup ; An, Se Woong ; Chun, Hee ;
Korean Journal of Agricultural Science, volume 43, issue 1, 2016, Pages 28~32
DOI : 10.7744/kjoas.20160004
This experiment was carried out to determine optimal culture conditions for the production of cut flowers and tubers of Calla (Zantedeschia `Black Magic`) in highlands. To achieve the research purpose, growth of `Black Magic` Calla influenced by tuber hardness (3.3 and
concentration (0, 100 and
), duration of
treatment (24 hr, 12 hr and 30min before planting) were investigated. When tubers have high hardness, those were not severely injured by soft rot disease regardless of
concentrations and treatment durations. Tubers with low tuber hardness showed more than 90% of soft rot occurrence when treated with
for 24 hrs before planting. However, soft rot did not occur when tubers were treated with
for 12 hrs before planting. In conclusion,
treatment results showed soft rot occurrence statistically significant degree in accordance with the bulbs hardness. In addition, the yield of the cut flowers is the result received the greatest effect in accordance with the bulbs in size and appeared to not be determined in accordance with the
treatment concentration and hardness bulbs. To obtain flowers without soft rot symptom, tubers (as
) should be completely dried after
Construction of a Full-length cDNA Library from Cardamine manshurica Nakai and Characterization of EST Dataset
Im, Subin ; Lee, Sung-Ho ; Kim, Yoon-Young ; Kim, Ju-Sang ; Kim, Dasom ; Lim, Yong Pyo ;
Korean Journal of Agricultural Science, volume 43, issue 1, 2016, Pages 33~39
DOI : 10.7744/kjoas.20160005
Brassicaceae consists of important species that have significant amounts of metabolites, and many studies have been carried out in order to understand the mechanism that improves the content of these metabolites. In Brassicacea, Cardamine manshurica Nakai is one of the important edible plants and is rich in oil, fiber, and various nutrients. In this study, we constructed cDNA library using leaves from 4 week-old plants and analyzed the ESTs of C. manshurica Nakai. One thousand thirty-nine ESTs were discovered which assembled to form 468 unigenes. The latter contained 116 contigs and 352 singletons. Similarity search of these ESTs with BLASTX revealed similarities with Arabidopsis thaliana 285 (31.9%), Arabidopsis lyrata 172 (19.3%), Capsella rubella 162 (18.1%), and Eutrema salsugineum 137 (15.3%). ESTs were functionally categorized into molecular function, biological process, and cellular component, and each category took 10.6%, 58.5%, and 30.9%, respectively. The functional analysis also found that 94.9% of ESTs showed at least one GO ID. Microsatellite analysis of 468 unigene sequences revealed 225 structures of which Di-, Tri-, Tetra-, Penta-repeats were 35.6% (80/225), 63.1% (142/225), 0.9% (2/225), and 0.4% (1/225), respectively. The results from our study can be a valuable resource for Cardamine research.
Change in settlement conditions of mountain area in Chungcheong region over a 10 year period : categorization of mountain villages
Lee, Bo-Hwi ; Kim, Se-Bin ; Kim, Uhn Soon ;
Korean Journal of Agricultural Science, volume 43, issue 1, 2016, Pages 40~51
DOI : 10.7744/kjoas.20160006
This study analyzed settlement condition changes of Chungcheong region`s mountain area during 10 years, from 2001 to 2012. We performed factor analysis and cluster analysis of data from the `Census for Mountain Area` carried out by the Korea Forest Service in 2001 and 2012. Factors 1, 2, 3, and 4 represent `the superiority of non-agricultural income`, `the industrialized mountainous area`, `residential conveniences and benefits`, and `the use of forest resources`, respectively. Clusters 1, 2, 3, 4, and 5 are categorized as `non-agricultural income`, `various mass social functions`, `production of forest products`, `industrialized mountainous region`, and `ordinary mountain region`, respectively. We suggest that cluster 1 has potential for development and should be promoted as a possible tourist attraction by digging up geographically unique themes. Cluster 2 has great potential for development and needs planned management through the maintenance or expansion of existing infrastructure. Cluster 3 has potential for development with various high value added industries uncovered. Cluster 4 shows vitality as it holds plenty of more job opportunities than other regions. Cluster 5 is deteriorating as a mountainous region because of an aging population, and it urgently demands development. For a decade, 45 of the 60 regions belonged to a single category, which are now differentiated broadly into two types: Firstly, deterioration changes to potential development and Secondly, vitality is differentiated into potential development and deterioration.
Follistatins have potential functional role in Porcine Embryogenesis
Kim, Dong-Hee ; Chun, Ju Lan ; Lee, Ji Hye ; Kim, Keun Jung ; Kim, Eun Young ; Lee, Bo Myeong ; Zhuang, Lili ; Kim, Min Kyu ;
Korean Journal of Agricultural Science, volume 43, issue 1, 2016, Pages 52~60
DOI : 10.7744/kjoas.20160007
In animal reproduction, the quality of oocytes and embryos has been evaluated by the expression of specific molecules. Follistatin (FST), which was isolated from follicular fluid, binds and bio-neutralizes the TGF-
superfamily members. Previous studies using the bovine model showed FST could be an important molecular determinant of embryo developmental competence. However, the effect of FST treatment on porcine embryo developmental competence has not been established. In this study, the effect of exogenous FST on porcine embryo developmental competence was investigated during in vitro culture. FST (10 ng/ml) treatment induced a significant decrease in the rate of cell arrest at the 4-cell stage. The expression levels of DNA-methyltransferase 1 (DNMT1), histone deacetylase 1 (HDAC1), and histone deacetylase 2 (HDAC2) were decreased in 4-cell stage embryos. FST treatment also resulted in significant improvements in developmental competence of embryos in terms of blastocyst formation rate and OCT-4 mRNA levels, the latter being related to pluripotency. In conclusion, during in vitro culture, FST treatment significantly ameliorated 4-cell block during embryonic development and improved embryo developmental competence. Therefore, FST treatment may potentially have a functional role in porcine embryogenesis that is broadly applicable to enhance in vitro embryo development.
The effect of aging on the quality of Semimembranosus muscle from Hanwoo
Kim, Sun Hyo ; Kim, Hyun Joo ; Lee, Hyun Jung ; Yong, Hae In ; Jo, Cheorun ; Nam, Ki Chang ; Jung, Samooel ;
Korean Journal of Agricultural Science, volume 43, issue 1, 2016, Pages 61~71
DOI : 10.7744/kjoas.20160008
The effect of postmortem aging on the quality of Semimembranosus muscle (SM) from Hanwoo was investigated. The quality of SM was compared with that of Longissimus dorsi muscle (LD). SM contained higher moisture, protein, and ash, and lower fat than LD (p<0.05). The
values of SM were significantly decreased after 21 days of aging. SM showed higher
values than LD at 1 and 7 day of aging (p<0.05). The cooking loss of SM was not affect by aging. SM had higher cooking loss than that of LD throughout the aging period. Although the shear force of SM was significantly decreased by aging, SM showed high shear force compared to LD during aging period (p<0.05). Aging increased lipid oxidation in both muscles (p<0.05). However, TBARS value of SM was significantly lower than that of LD throughout the aging period (p<0.05). There was no effect of aging on the betaine and L-carnitine contents of SM. SM contained higher betaine and L-carnitine than LD in all aging days (p<0.05). Therefore, we concluded that consumer acceptability of SM could be increased by aging based on the increase of tenderness in addition to its high nutritional properties.
Effect of metabolic imprinting on growth and development in piglets
Ryu, Jae-Hyoung ; Lee, Yoo-Kyung ; Cho, Sung-Back ; Hwang, Ok-Hwa ; Park, Sung-Kwon ;
Korean Journal of Agricultural Science, volume 43, issue 1, 2016, Pages 72~79
DOI : 10.7744/kjoas.20160009
It has long been known that nutritional and environmental influences during the early developmental period affect the biological mechanisms which determine animal metabolism. This phenomenon, termed `metabolic imprinting`, can cause subtle but long-lasting responses to prenatal and postnatal nutrition and even be passed onto the next generation. A large amount of research data shows that nutrient availability, in terms of quantity as well as quality, during the early developing stages can decrease the number of newborn piglets and their body weight and increase their susceptibility to death before weaning. However, investigation of potential mechanisms of `the metabolic imprinting` effect have been scant. Therefore, it remains unknown which factors are responsible for embryonic and early postnatal nutrition and which factors are major determinants of body weight and number of new born piglets. Intrauterine undernutrition, for example, was studied using a rat model providing dams 50% restricted nutrients during pregnancy and the results showed significant decreases in birth weight of newborns. This response may be a characteristic of a subset of modulations in embryonic development which is caused by the metabolic imprinting. Underlying mechanisms of intrauterine undernutrition and growth retardation can be explained in part by epigenetics. Epigenetics modulate animal phenotypes without changes in DNA sequences. Epigenetic modifications include DNA methylation, chromatin modification and small non-coding RNA-associated gene silencing. Precise mechanisms must be identified at the morphologic, cellular, and molecular levels by using interdisciplinary nutrigenomics approaches to increase pig production. Experimental approaches for explaining these potential mechanisms will be discussed in this review.
Ruminal microbial responses in fermentation characteristics and dry matter degradability to TDN level of total mixed ration
Lee, Seung-Uk ; Jo, Jin-Ho ; Park, Sung-Kwon ; Choi, Chang-Weon ; Jeong, Jun ; Chung, Ki-Young ; Chang, Sun-Sik ; Li, Xiang Zi ; Choi, Seong-Ho ;
Korean Journal of Agricultural Science, volume 43, issue 1, 2016, Pages 80~86
DOI : 10.7744/kjoas.20160010
An in vitro trial was conducted to examine the effects of total mixed rations (TMR) on fermentation characteristics and effective degradability (ED) by rumen microbes. Three TMR diets were growing period TMR (GR-TMR, 67% TDN), early fattening period TMR (EF-TMR, 75.4% TDN) and late fattening TMR (LF-TMR, 80% TDN). Three TMR diets (3 g of TMRs in each incubation bottles) was added to the mixed culture solution of stained rumen fluid with artificial saliva (1:1, v/v) and incubated anaerobically for 48 hours at
. The pH in all incubation solutions tended to decrease up to 48h, but the opposite results were found in concentration of total gas production, ammonia-N and total VFA in all incubations.The total gas production (p<0.05) in LF-TMR was highest compared with those of other diets. Also, concentration of total VFA was tended to increase in LF-TMR compared with other TMR diets in all incubations. The EDDM in both EF-TMR and LF-TMR was tended to high compared with GR-TMR (p
Effect of cooking methods on the phytosterol content in nine selected vegetables
Shin, Jung-Ah ; Park, Jong-Min ; Lee, Ki-Teak ;
Korean Journal of Agricultural Science, volume 43, issue 1, 2016, Pages 87~99
DOI : 10.7744/kjoas.20160011
Phytosterol contents in nine vegetables such as paprika (red, yellow, and orange), kohlrabi, bamboo shoot, cherry tomato, cucumber, Chinese chive, and corn were analyzed by gas chromatography. Individual contents of
-sitosterol, campesterol, and stigmasterol in fresh and cooked vegetables (boiling, grilling, stir-frying, deep-frying, steaming, roasting, and microwaving) were determined and compared. Total phytosterol content of paprika, cucumber, Chinese chive and cherry tomato ranged from 23.19 to 46.51 mg/kg (0.002-0.005%) of fresh weight of raw vegetables. Total phytosterol content variation (%) was obtained as follows: [(the content of phytosterol after cooking) - (the content of phytosterol before cooking)] / (the content of phytosterol before cooking)
. Total phytosterol content was found to be high in raw kohlrabi at 138.99 mg/kg fw (0.01%), in corn at 302.86 mg/kg fw (0.03%), and in bamboo shoot at 443.15 mg/kg fw (0.04 %). Total phytosterol content variation (%) in orange paprika ranged from 27.5 to 267.3 while that in cherry tomato ranged from -11.0 to 337.5. Generally, high content variation of total phytosterol was found in stir-fried and deep-fried vegetables. Therefore, higher phytosterol levels were obtained from cooked vegetables than raw vegetables. We suggest that these data will be useful to investigate cooking methods for increased intake of phytosterols.
Isolation and Characterization of Plant Growth Promoting Rhizobacteria From Button Mushroom Compost
Oh, Sung-Hoon ; Lee, Chang-Jung ; Yoon, Min-Ho ;
Korean Journal of Agricultural Science, volume 43, issue 1, 2016, Pages 100~108
DOI : 10.7744/kjoas.20160012
An auxin-producing bacteria (strain 5-1) was isolated from button mushroom compost in Boryeong-Si, Chungcheongnam-Do. The 5-1 strain was classified as a novel strain of Enterobacter aerogenes based on chemotaxonomic and phylogenetic analyses. The isolated E. aerogenes 5-1 was confirmed to produce indole-3-acetic acid (IAA), one of the auxin hormones, using TLC and HPLC analyses. When the concentration of IAA was assessed by performing HPLC quantitative analysis, a maximum concentration of IAA of
was detected in the culture broth incubated in R2A medium containing 0.1% L-tryptophan for 24 h at
. Acidification of the culture was deemed caused by an increase of IAA because a negative relationship between IAA production and pH was observed. Supplementation with a known precursor of IAA production, L-tryptophan, appeared to induce maximal production at 0.1% concentration, but it reduced production at concentrations above 0.2%. To investigate the growth-promoting effects to crops, the culture broth of E. aerogenes 5-1 was used to inoculate water cultures and seed pots of mung bean and lettuce. In consequence, adventitious root induction and root growth of mung bean and lettuce were two times higher than those of the control.
Single-dose oral toxicity study of genetically modified silkworm expressing EGFP protein in ICR mouse
Jang, Kyung-Min ; Kim, Sung-Gun ; Park, Ji-Young ; Choi, Won-Ho ; Lee, Jae-Woo ; Jegal, Hyeon-Young ; Kweon, Soon-Jong ; Choi, Kwang-Ho ; Park, Jung-Ho ;
Korean Journal of Agricultural Science, volume 43, issue 1, 2016, Pages 109~115
DOI : 10.7744/kjoas.20160013
Silk has had a reputation as a luxurious and sensuous fabric but it is not popular due to the expensive price and poor durability. To develop the silk materials that apply the various industries, the artificially synthesized gene can be introduced into the silkworm and expressed in the silk gland. Transgenic silkworms for the mass production of green fluorescent silks are generated using a fibroin H-chain expression system. For commercial use, safety assessment of the transgenic silkworms is essential. The purpose of this study was to examine the potential acute oral toxicity of EGFP protein expressed in genetically modified (GM) fluorescence silkworm and to obtain the approximative lethal dose in the male and female at 6-weeks ICR mice. EGFP protein was fed at a dose of 2,000 mg/kg body weight in five male or five female mice. Mortalities, clinical findings and body weight changes were monitored for 1, 3, 7, 14 days after dosing. At the end of 14 day observation period, all mice were sacrificed, and the postmortem necropsy were performed. The test group was not observed death case. Also the effect was not admitted by test substance administration in common symptoms, the body weight and postmortem. The results of single-dose oral toxicity test showed that approximative lethal dose of EGFP protein expressed in fluorescence silkworm was considered to exceed the 2,000 mg/kg body weight in both sexes.
Development of simulation model for fuel efficiency of agricultural tractor
Kim, Wan-Soo ; Kim, Yong-Joo ; Chung, Sun-Ok ; Lee, Dae-Hyun ; Choi, Chang-Hyun ; Yoon, Young-Whan ;
Korean Journal of Agricultural Science, volume 43, issue 1, 2016, Pages 116~126
DOI : 10.7744/kjoas.20160014
The objective of this study is to predict the fuel efficiency of an agricultural tractor. The fuel efficiency of the tractor during rotary tillage was predicted using numerical modeling. A numerical model was developed using Simulation X. Based on tractor power flow, numerical modeling consisted of an engine, transmission, PTO (power take off), and hydraulics. The specifications of major components utilized in the numerical model were the same as those of a 71 kW tractor (field test tractor). The load that was inputted for fuel efficiency prediction into the simulation model was obtained from a field test. Fuel efficiency predictions were conducted by comparing field test results and simulation results. In addition, it was performed by dividing the rotary tillage and steering section. Main results are as follows: first, t-values of engine torque were measured to be 0.31 in the rotary tillage and 0.92 in the steering section. Second, t-values of fuel consumption were measured to be 0.51 and 5.41 in the rotary tillage and the steering section, respectively. Finally, t-values of fuel efficiency were measured to be 1.72 and 40 in the rotary tillage and the steering section, respectively. The results show no significant differences with t-values of less than 5% in the rotary tillage. But, it shows significant differences in the steering section. Therefore, simulation for accurate fuel efficiency prediction requires a suitable algorithm or detailed design of the simulation model in the steering section.
An analysis of the relationship between farming capability of farmers and farm Household Income
Seo, Jeongwon ; Kim, Yoonhyung ;
Korean Journal of Agricultural Science, volume 43, issue 1, 2016, Pages 127~135
DOI : 10.7744/kjoas.20160015
Improving farming activity competence of farm households has recently been considered one of the most important factors for increasing farm income. However, few studies examine the relationship between farm income and farming activity competence of farm households directly due to the lack of an available dataset. In this study, we examine the relationship between farm household technical managerial competence and farm household income based on the nearly 30,000 farm households consulting data gathered by the Rural Development Administration, RDA. The major findings of this study are as follows: firstly, statistically significant differences in agricultural and farm household income exist between farm households categorized by farm activity competence levels in terms of technique and management. Secondly, a technically and managerially competent farm household group (high-rank farm household) has 2.2 times higher agricultural income and 1.9 times higher farm household income than the technically and managerially incompetent farm household group (low-rank farm household). Thirdly, farm household technical-managerial competence is one of the major factors that affect agricultural and farm household income. Regarding technical competence, agricultural income and farm household income increased by approximately 1,390,000 won and 1,530,000 won, respectively, as technical points increased by one point. However, with respect to managerial competence, agricultural income and farm household income increased by approximately 1,320,000 won and 2,070,000 won, respectively, as managerial points increased by one point.
Analysis of the relationship between garlic and onion acreage response
Lee, Eulkyeong ; Hong, Seungjee ;
Korean Journal of Agricultural Science, volume 43, issue 1, 2016, Pages 136~143
DOI : 10.7744/kjoas.20160016
Garlic and onion are staple agricultural products to Koreans and also are important with regard to agricultural producers` income. These products` acreage responses are highly correlated with each other. Therefore, it is necessary to test whether there is a cointegration relationship between garlic acreage and onion acreage when one tries to estimate the acreage response`s function. Based upon the test result of cointegration, it is confirmed that there is no statistically significant cointegration relationship between garlic acreage and onion acreage. In this case, vector autoregressive model is preferred to vector error correction model. This study investigated the dynamic relationship between garlic and onion acreage responses using vector autoregressive (VAR) model. The estimated results of VAR acreage response models show that there is a statistically significant relationship between current and lagged acreage of more than one lag. Therefore, it is recommended that government should consider the long-run period`s relationship of each product`s acreage when it plans a policy for stabilizing the supply and demand of garlic and onion. For the price variables, garlic price only affects garlic acreage response while onion price affects not only onion acreage response but also garlic acreage response. This implies that the stabilizing policy for onion price could have bigger effects than that for garlic price stabilization.
An importance-performance analysis of using farmers in a rice co-nursery : Focus on the East Gunsan Agricultural Cooperative
Han, Sang-Don ; Yun, Sung-Hwan ; Jang, Dong-Heon ;
Korean Journal of Agricultural Science, volume 43, issue 1, 2016, Pages 144~151
DOI : 10.7744/kjoas.20160017
With an aging population in rural areas, farm owners in South Korea are also aging. In particular, this phenomenon leads to the lack of human resources in rural areas and agriculture, worsening the deterioration of the rural economy. This study aimed, firstly, to analyze the criticality of the lack of human resources and high production cost issues for farmers, before using the farm as cooperative nursery of rice seedlings. Secondly, the study analyzed farmer satisfaction after using the farm cooperative. Analysis results are as follows. The motives of the research subjects for using the co-nursery were the aging of the farming population, the expected reduction of production costs, and farmers` failure in raising seedlings. Importance-performance analysis measured the importance for the farms for cooperative raising of rice seedling at 3.49 and the performance for the farms at 3.41, with a difference of 0.07 between them. The four importance-performance matrix areas, used were as follows: concentrated efforts required (Quadrant I: concentrate here), continuous maintenance (Quadrant II: keep up the good work), gradual improvement (Quadrant III: low priority), and avoiding excessive efforts (Quadrant IV: possible overkill). Based on the results, it was concluded that improvements and efforts would be necessary for each of these areas. And, there is a need to improve services through the initiation of farmer use of this rice co-nursery.