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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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The Korea Journal of Herbology
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Journal DOI :
The Korea Association of Herbology
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Volume & Issues
Volume 17, Issue 2 - 00 2002
Volume 17, Issue 1 - 00 2002
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Comparative Study of Efficacy of Foeniculi Fructus and Anethi Fructus Extract on Analgesic Effects, Anti-inflammatory Effects and Arthritis
Ban, Sang-Seok ; Park, Ji-Ha ; Lee, Eun-Suk ; Choi Hae-Yun ; Lee, Byeong-Uk ; Seo, Bu-Il ;
The Korea Journal of Herbology, volume 17, issue 2, 2002, Pages 1~1
Objectives : This experimental study was done to compare the efficacy of both Foeniculi Fructus and Anethi Fructus extract on analgesic effects, anti-inflammatory effects and arthritis. Methods : We studied the writhing syndrome in mice induced by 0.7% acetic acid for analgesic effects. We measured inhibitory effects on the paw-edema by acetic acid in rats to elucidate anti-inflammatory effects. And we studied the method of adjuvant arthritis to elucidate effects on arthritis. Results : In analgesic action on the writhing syndrome in mice induced by 0.7% acetic acid, Foeniculi Fructus and Anethi Fructus extract treated group showed significant decrease in cimparison with control group. In anti-inflammatory effects, Foeniculi Fructus extract treated group showed significant decrease at 180 minutes after inducing paw-edema in comparison with control group. And Anethi Fructus group showed significant decrease at 30 and 120 minutes after inducing paw-edema in comparison with control group. In the method of dajuvant arthritis, they significantly inhibited the hind paw edema in rats with Foeniculi Fructus extract from 15 days later to 21 days later after administration. And Anethi Fructus group extract treated group showed significant decrease at 7 days later after administration. In the level of leucocytes count and total protein, Foeniculi Fructus and Anethi Fructus extract treated group showed increase in comparison with control group, but these results didn't show significant increase. In the level of CRP, Foeniculi Fructus and Anethi Fructus extract treated group showed decrease in 00comparison with control group, but these results didn't show significant decrease. In the level of glucose and platelet count, Foeniculi Fructus extract treated group showed increase in comparison with control group, but these results didn't show significant increase. Anethi Fructus extract treated group showed decrease in comparison with control group, but these results didn't show significant decrease. Conclusion : Reviewing these experimental results, it appears that Foeniculi Fructus and Anethi Fructus Extract have equal efficacy on analgesic effects, anti-inflammatory effects and arthritis.
Analysis of Linoleic Acid on the Extraction Method of arcti Fructus
Jo, Yun-Hui ; Seong, Gyeong-Hwa ; Lee, Eun-Ju ; An, Deok-Gyun ; Park, Seong-Gyu ;
The Korea Journal of Herbology, volume 17, issue 2, 2002, Pages 11~11
objectives : Arcti Fructus has been used for disperses Wind Heat and benefits the throat, detoxifies Fire Poison and encourages rashes to surface, Polyunsaturated fatty acids(PUFAs), especially linoleic acid,
-linolenic acid, arachidonic acid, are efficacious in treating skin disorders. methods : We analyzed the composition of PUFAs in Arcti Fructus. The analysis of PUFAs such as linoleic acid,
-linolenic acid, arachidonic acid from the organic extract or reflux water extract of Arcti Fructus was conducted by Gas Chromatography with SPB-225 Fused Silica Capillary column. results : Of several PUFAs, Arcti Fructus contains linoleic acid only. Although the contents of linoleic acid from reflux water extract of Arcti Fructus was maximized after 3hr treatment, organic extract contains absolutely higher level of linoleic acid than reflux water extract. conclusion : Grinding process enhanced the contents of linoleic acid in reflux water extract of Arcti Fructus after 2hr treatment.
Effects of Platycodon grandiflorum powder on Body Weight and Serum Biological Composition of Obese Rats Fed High Fat Diet
Jin, Cheol-Yong ; Byeon, Bu-Hyeong ; Park, Ji-Ha ; Lee, Eun-Suk ; Choi Hae-Yun ; Lee, Byeong-Uk ; Seo, Bu-Il ;
The Korea Journal of Herbology, volume 17, issue 2, 2002, Pages 19~19
Objectives : This experimental study was designed to investigate the effects of Platycodon grandiflorum powder on body weight and serum biological composition. Methods : A series of experiments have been conducted in order to measure the effects of the body weight changes and serum lipid profiles. The measurement have been performed on; (1) the changes amount of body weight; (2) the quantity of total cholesterol, triglyceride, free fatty acid in serum. Results : Rats given feed containing high fat and Platycodon grandiflorum powder showed significant decrease in net weight gain in comparison with high fat diet feeding group. The quantity of total cholesterol, triglyceride, free fatty acid in serum was significant decrease in high fat and Platycodon grandiflorum powder group in comparison with that of high fat feeding group. Conclusion : Results of our research in this paper show that Platycodon frandiflorum powder might improve lipid composition and body wight in rats Fed with high fat diet
Studies on Biological Activity from Extract of Crataegi Fructus
An, Bong-Jeon ; Lee, Jin-Tae ;
The Korea Journal of Herbology, volume 17, issue 2, 2002, Pages 29~29
Objectives : This is the study of the application as the ingredients of cosmetics through the examination of the function for physiological activity of Craraegi Fructus, which has been used for herb medicines since ancient times. Method : Korean Craraegi Fructus, which had been extracted, concentrated, and freeze drying with water and ethanol, have been used for the experiment. The effects on elecrtonic donating ability, SOD-like activity, whitening effect, gout and hypertension prevention, Melanoma cell have been researched in the physiological activity measurement of function experiment. Results : According to the physiological activity measurement of function experiment, it have been found that the electronic donating ability has higher effect than BHT, synthetic antioxidant, showing 89.4%~91.8% at 500 ppm. As the concentration increases, the inhibitory effect showed a tendency to rise. However the effect was not so high. Tyrosinase, which is correlated with whitening effect, showed 49.8%~62.4% of the high inhibition ratio at 30,000 ppm. ACE inhibitory effect, which is correlated with hypertension prevention was 32.7%~34.5% at 10ppm but there was no distinctive difference by concentration. According to the result of astringent effect, the ethanol extract from Craregi Fructus showing 89% at 5,000 ppm brought the similar effect to Gerannii Herba and SK_MEL-5, melanoma cell, showed 68.9%~89% of sudden growth inhibition of cell at 50000 ppm, and 10000 ppm. Conclusion : According to the study of the application as cosmetic ingredients, Crataegi Fructus extract has enough merits as a new biological activity ingredient and it will be very effective in the cosmetic industry as the affinitive natural product.
Development of Cosmetic Material from Korean Crataegi Fructus Extract
An, Bong-Jeon ; Gang, Bo-Yeon ; Lee, Jin-Tae ;
The Korea Journal of Herbology, volume 17, issue 2, 2002, Pages 39~39
Objectives : This is the study of the application as the ingredients of cosmetics through the examination of the function for physiological activity of Crataegi Fructus, which has been used for herb medicines since ancient times. Method : Korean Crataegi Fructus was extracted by water and ethanol and it was concentrated for the experiment materials. As the stability of the physiological activity function experiment, the effect of the electronic donating ability, antioxidative activity and UV scanning depending on various moisturizers has been researched for the development of cosmetic ingredients. Results : The ethanol extract form Crataegi Fructus showed similar antioxidative activity, reaching 81.2% at 1000 ppm. As the result of UV scanning of each extract at 1000 ppm concentration and in UV 200~400 nm, it showed the similar absorbent peak (O.D: 0.7~0.8) to green tea extract, rosemary extract in UVB zone (280 nm) and the high optical density (1.8~2.4) in UVC zone. Propylene Glycol showed 71.8~75% antioxidative activity at 1mg/mL, and Glycerin showed 70.7%~71.8%, while 1,3-Butylene Glycol showed the safest antioxidative activity, reaching 79%~85%. Conclusion : According to the result from the electronic donating ability measure of Normal skin-softener of the basic formulation, using 1,3-Butylen Glycol and various extracts from natural plants, it can be proved that Crataegi Fructus extract has the similar antioxidative activities degree of other extracts.
Physiological activity of Salicornia herbacea and Its application for Cosmetic materials
Lee, Jin-Tae ; Jeong, Yeon-Suk ; An, Bong-Jeon ;
The Korea Journal of Herbology, volume 17, issue 2, 2002, Pages 51~51
Objectives : Salicorna herbacea is not well known in research except hat it contains plenty of minerals. This is the first report that showed bioactive functions of the extract from Salicornia herbacea, and examined the possibility to develop as 2a new cosmetic materials. Methods : Hot Water Extract(W.E), Ethanol Extract(E.E) and Crude Extract(C.E) were used as samples. Using Sephadex LH-20, Hot Water extract fractions and Ethanol extract fractions were gained that used for the experiments. Results : In searching for antibacterial activity, the halos for Propionibacterium acnes which are causal bacteria for pimples was identified in 5,000 ppm and 10,000 ppm. In fraction 1 of E.E, it showed 68% Electron Donating Ability(EDA), 61% of tyrosinase inhibiton effect and 50% of Angiotensin Converting Enzyme Ⅰ (AEC) inhibition effect in 10,000 ppm, respectively. In the measurement of astringent effect, W.E of Salicornia herbacea revealed 50% of astringent effect in 10,000 ppm. In the experiment of growth inhibiting ability for human melanoma SK-MEL-5, 5,000 ppm W.E showed over 60% of growth inhibiting ability. Conclusion : It was identified EDA, tyrosinase, astringent could be used for cosmetic materials, and melanoma growth inhibiting ability for the skin and ACE hindrance effect for medical materials.
Detection of Physical activity of Salicornia herbacea
Lee, Jin-Tae ; An, Bong-Jeon ;
The Korea Journal of Herbology, volume 17, issue 2, 2002, Pages 61~61
Objectives : Salicornia herbacea is an internal salt plant that grows in the western coast, southern coast, beaches, and salt fields in Korea peninsula. Belonging to 2Chenopodiaceae by a biological classification, Salicorna herbacea is not known from research except that it contains plenty of minerals. Therefore, this paper reported bioactive functions of the extract from Salicornia herbacea, and examined the possibility to develop as a new industrial materials. Methods and Results : Hot Water Extract(W.E), Ethanol Extract(E.E) and Crude Extract(C.E) were used as samples. In searching for antibacterial activity, Clear zone for Propionibacterium acnes which are causal bacteria for pimples was identified in 5,000 ppm and 10,000 ppm. E.E showed 97% Electron Donating Ability(EDA), 61% of tyrosinase inhibiton effect, respectively. C.E showed 50% of Angiotensin Converting Enzyme Ⅰ (ACE) inhibition effect and 96% of xanthine oxidase inhibition in 1,000ppm, respectively. Conclusion : As the results of the experiments, it was identified that Electron Donating Ability, tyrosinase inhibition could be used for cosmetic materials, and melanoma growth inhibiting ability for the skin and ACE hindrance effect for medical materials.
Isolation and Identification of Soil Microorganisms in Wild Ginseng Radix of Moo-Ju Area
Sin, Dong-Yeop ; Park, Yong-Il ; Kim, Hong-Ryeol ; An, Deok-Gyun ; Park, Seong-Gyu ;
The Korea Journal of Herbology, volume 17, issue 2, 2002, Pages 71~71
objectives : Microorganisms were isolated from soils remaining attached to the roots of Wild Panax ginseng C.A. Mey. naturally grown in Moo-Ju area. methods : As a preliminary study for the establishment of wild ginseng radix discrimination system based on area of growth, these isolates were identified by using mainly API 20NE and API 50CHB kits and the microbial consortia were compared. From the sample of Moo-Ju area, 9 and 6 strains were isolated and identified after the incubation of agar plates at 30
, respectively. results : The results showed that 2 strains of soil bacteria, Aeromonas hydrophilia, and Bacillus cereus commonly existed in the roots of wild ginseng of both 30
. And that 6 strains, Pseudomonas putida, Stenotrophomonas maltophilia, Pseudomonas fluorescence at 30
and Bacillus subtilis, Empedobacter brevis, Brevi. laterosporus at 37
, inhabited specifically in the roots of wild ginseng of Moo-Ju area. conclusion : These results suggest that comparison of microbial consortia associated with roots of wild ginseng could be used for the establishment of a naturally growing wild ginseng radix discrimination system.
Effect of Sasunchungryangeum on Acute Edematous Lung Injury Induced by Skin Burn
Choi Jin-Yong ; Gang, Byeong-Su ; Jo, Hyeon-Guk ;
The Korea Journal of Herbology, volume 17, issue 2, 2002, Pages 85~85
Objectives : The present study was conducted to determine whether administration of heat extracts of Sasunchungtyangeum has an inhibitory effect of neutrophil-derived oxidative injury following dermal scald burn in rats. Methods: Acute lung injury was induced by scald burn (15% of TBSA) in rats. To identify acute lung edematous lung injury, 5 hrs after skin burn, protein concentrations and numbers of polymorphonuclear leukocytes in bronchoalveolar lavage (BAL) were measured. In addition, levels of lung KC (neutrophil chemoattractant cytokine) and activities of lung myeloperoxidas (MPO) were measured, and morphological changes were observed. Results : Lung weight and concentration of BAL protein, the index of lung injury were clearly increased at 5 hrs postburn compared to them of sham. Administration of heat extracts of Sasunchunryangeum after scald burn inhibited the production of KC in lung tissue and decreased the activity of lung MPO related to influx of neutrophils. In histological changes, edematous lung injury with infiltration of inflammatory cells induced by skin burn were decreased by administration of heat extracts of Sasunchunryangeum after scald burn. Conclusions : These results suggest that Sasunchunryangeum may be a successful medical stuff in acute lung injury by skin burn.
The Immunological Activities of Kyungohkgo and Prescription of Modified Kyungohkgo
Lee, Eun-Suk ; Seo, Bu-Il ; Lee, Jun-U ; Bae, Jin-Seung ;
The Korea Journal of Herbology, volume 17, issue 2, 2002, Pages 95~95
Objectives : This study was conducted to investigate the immunological activities of Kyungohkgo and prescription of modified Kyungohkgo. Methods : Raw 264.7 macrophage treated with Kyungohkgo or other simuli enhanced production of the nitric oxide and TNF-
. Results : Among the sample 1. sample 2 and 3 of Kyungohkgo, sample 2 exerted more favorable influence on nitric oxide(NO) production than the sample 3. And production of NO and TNF-
were dependent on the concentration of Kyungohkgo. Conclusion : These results suggested that Kyungohkgo-activated macrophage induce the NO and TNF-
and its containing in immunoregulatory components.
Effects of Kyungohkgo and prescription of Modified Kyungohkgo on lung Cancer
Lee, Eun-Suk ; Seo, Bu-Il ; Lee, Jun-U ; Bae, Jin-Seung ;
The Korea Journal of Herbology, volume 17, issue 2, 2002, Pages 101~101
kyungohkgoes (sample 2 and sample 3) when they prescribed to mice that translate a 3LL lung cancer cell. A sample 1, 2, and 3 are prescribed for the 1st, 2nd and 3rd group, respectively. Methods : Inoculate mouse lung carcinoma cells to a BDFI mouse and did an experiment about tumor occurrence periods, body weight, tumor volume, survival rate, life prolongation. Results : The development time of tumor lump to the 3rd group is latest as 181.71
18.88 hours. That is, it means that a lung cancer of this group was induced latest. In tumor size, the 3rd group is also fewest by 1,536~9,375
. The tumor growth rate of the 3rd group appeared lowest by 610.35 percent. Only the 3rd group was appeared that has a control effect of tumor growth. Also, 3 mice of the 3rd group were survived for 30day after translating the lung cancer cells. The survival extension rate of the 3rd group was highest by 38.39 percent. Conclusion : In these experiments, it appears that the sample 3 prescribed by modified kyungohkgo has the highest anti-cancer effect. It is proved that the modified kyungohkgo affects the 3LL lung cancer cell by acting complexly.
Vasodilation Effect of the Water Extract of Rheum palmatum L. in Rat Thoracic Aorta
Kim, Hyeong-Hwan ; Gu, Bon-Sik ; Park, Su-Yeon ; An, Deok-Gyun ; Choi Ho-Yeong ; Park, Seong-Gyu ;
The Korea Journal of Herbology, volume 17, issue 2, 2002, Pages 111~111
Objectives : We have examined the relaxational response to the water extract of Rheum palmatum L. in isolated thoracic aorta from sprague dawley (SD) rat in the presence and absence of endothelium. Methods : Rat thoracic aorta was investigated in vessel segments suspended for isometric tension recording by polygraph. Responses to Rhizoma Rhei were investigated in vessels precontracted with 5-hydroxytryptamine. We found that the thoracic aorta segments responded to the water extract of Rheum palmatum L. (ERP) with a dose-dependent vasorelaxation. Results : We found that 1. The thoracic aorta segments responded to ERP with a dose-dependent vasodilation. 2. The 5-HT induced contraction at 10^-4M were inhibited by 85.8% after addition of the 0.1 g/mL water extract of ERP. 3. The 5-HT induced contraction at 10^-4M with and without endothelium were inhibited by 86.4% and 85.8% after addition of the 0.1g/mL ERP. 4. After pre-treatment of the thoracic aorta with 10^-4M N^G-monomethyl-L-arginine (L-NMMA), inducible nitric oxide synthase inhibitor, the vessels has not response to the contraction. Conclusion : In conclusion, ERP induced relaxation in the isolated rat thoracic aorta were composed of dose-dependent relaxation and it has potent vasodilation.
Effect of Sophorae Radix on Methylmercury-Induced Myotoxicity in Cultured Myocardial Cells
Song, Ho-Jun ; Ha, Dae-Ho ; Yu, Gyo-Sang ; Park, Seung-Taek ; Lee, Gang-Chang ; Seo, Bu-Il ;
The Korea Journal of Herbology, volume 17, issue 2, 2002, Pages 119~119
Objectives : To evaluate the toxic effect of methylmercuric chloride(MMC) and the protective effect of Sophorae Radix(SR) in cultured mouse myocardial cells, cytotoxicity was measured by MTT assay Methods : Cultured myocardial cells were incubated for 72 hours in the media containing 5~40 ㎛ concentrations of MMC. And also, the protective effect of SR was determined by sulforhodamine B(SRB) for protein sythesis and radioactive assay for DNA sythesis in these cultures. Results : Cell viability was significantly decreased in a dose-dependent manner after cultured myocardial cells were exposed to 20 ㎛ MMC for 72 hours. In the neuroprotective effect of SR on MMC-induced cytotoxicity, SR blocked the MMC-induced myotoxocity in these cultures. Conclusion : It suggests that MMC is toxic against cultured mouse myocardial cells and SR is effective in blocking the meurotoxicity induced by MMC.
Effect of Glucose oxidase and Effect of Gastrodiae Rhizoma on Glucose Oxidase in Spinal Sensory Ganglion Neurons
Song, Ho-Jun ; Lee, Yong-Seok ; Son Yeong-Woo ; Lee, Gang-Chang ; Jeong, Jong-Gil ; Sin, Min-Gyo ; Hong, Gi-Yeon ;
The Korea Journal of Herbology, volume 17, issue 2, 2002, Pages 125~125
Objectives : To clarify the cytotoxic effect of glucose oxidase(GO) and protective effect of gastrodiae Rhizoma (GR) on spinal sensory ganglion(DRG) neurons, neurotoxicity mediated by GO was measured by MTT assay and neurofilament enzymeimmuno assay(EIA). Methods : DRG neurons were cultured in the media containing various concentrations of GO for 3 hours. In addition, neuroprotective effects of GR, on GO-induced neurotoxicity in DRG neurons were examined after DRG neurons were preincubated with various concentrations of GR for 2 hours before 15mU/ml GO for 3 hours. Results : GO decreased remarkably cell viability in dependently in these cultures, and also RG increased cell viability and amount of neurofilament in DRG neurons damaged by GO. Conclusion : It is suggested that GO has toxic effect in cultured mouse DRG neurons, and also RG was effective in the protection of GO-induced neurotoxicity in these cultures.
Effect of Cibotii Rhizoma on the Growth Hormone Secretion of Adolescent Male Rats
Im, Gang-Hyeon ; Bu, Yeong-Min ; Park, Seon-Yeong ; Lee, Dong-Hwan ; Kim, Ho-Cheol ;
The Korea Journal of Herbology, volume 17, issue 2, 2002, Pages 133~133
Effect of Gypsum Fibrosum on Interleukin-4 Secretion of Mice Splenocytes
Im, Gang-Hyeon ; Jo, Jeong-Je ; An, Hyeon-Jong ; Yu, Yeong-Cheon ; Kim, Seong-U ; Kim, Hyeon-Mi ; Kim, Jeong-Seon ;
The Korea Journal of Herbology, volume 17, issue 2, 2002, Pages 139~139
Quantitative Determination of 5-Hydroxymethyl-2-furaldehyde from Rehmanniae Radix Preparata according to Various Processings
Lee, Je-Hyeon ; Go, Jeong-A ; Hwang, Eun-Yeong ; Hong, Seon-Pyo ;
The Korea Journal of Herbology, volume 17, issue 2, 2002, Pages 145~145
Objectives : Rehmanniae Radix Preparata has been used to enrich blood and supply yin for a several thousand years. We have studied on the quantity of 5-hydroxymethyl-2-furaldehyde (5-HMF) from Rehmanniae Radix Preparata at various processings and tried to develop the making method. Methods : The Rehmanniae Radix Preparata was prepared from the steaming process with rice wine, sterilized rice wine, diluted ethanol and water each other. And the change of 5-HMF was determinated. The analysis of 5-HMF was conducted by HPLC with reversed-phase C18 column and a UV detector at 280 nm. Elution was carried out at 1.0 ml/min with 5% acetonitrile. Results and Conclusions : From this analysis, we found out that the content of 5-HMF was increased with the number of processing times and the content of 5-HMF from Rehmanniae Radix Preparata steamed for 4 times was the same as that of Korea pharmacopoeia.
Neuroprotective Effect of Angelica gigas Extracts on the Brain Ischemia Induced by Four-Vessel Occlusion in Rats
Kim, Yeong-Ok ; Ha, Ni-Na ; Bu, Yeong-Min ; Park, Seon-Yeong ; Park, Ju-Yeong ; Yu, Yeong-Beop ; Sin, Jun-Sik ; Kim, Ho-Cheol ; An, Deok-Gyun ;
The Korea Journal of Herbology, volume 17, issue 2, 2002, Pages 151~151
Objectives : Angelicae Radix, the root of Angelica gigas N_AKAI (Umbelliferae) , which has been used to nourish the blood(補血), regulate menstruation(調經), relieve pain and promote bowel movements(崩漏) and activate blood circulation for the treatment of fatigue, headache, constipation and blood troubles in traditional Korean medicine. It's flavour and channel tropism is sweet(甘), pungent(辛), warm(溫), acting on the lung, heart and spleen(肺, 心, 脾). The purpose of study we report here was to determine the neuroprotective effect of Angelica gigas on global ischemia induced by 4-vessel occlusion in Wistar rats. Methods : Angelica gigas extracts was lyphilized after extraction with 85% methanol. We induced 4-VO for 10 minutes and reperfused again. The number of CA1 pyramidal neurons were counted after 7 days of reperfusion under the cresyl violet staining. Results : The results showed that Angelica gigas had significantly neuroprotective effects (100, 250 and 500mg/kg of AR extracts, p＜0.001) compared with control group. Each neuroprotective ratio was about 66.1, 79.3 and 78.6% respectively. Immunohistochemical data partially explained the protective effects of Angelica gigas via attenuation of COX-2 induction in hippocampus. Conclusion : Angelica gigas gas neuroprotective effects on the global ischemia induced by 4-vessel occlusion in Wistar rats and the attenuation of COX-2 induction is thought to be its possible mechanism.
Transformation of Antioxidant Ascorbate Peroxidase Gene
Lee, Eun-Suk ; Seo, Bu-Il ; Lee, In-Ae ;
The Korea Journal of Herbology, volume 17, issue 2, 2002, Pages 159~159
Objectives : An experiment was carried out to introduce Apx8, ApxSC gene isolated from strawberry to orchardgrass(Dactylis glomerata L.) using Agrobacterium. Methods : Mature seed-derived calli of orchardgrass were co-cultured with Agrobacterium tumefaciens EHA101 harboring the plasmid pIG-Apx8 for transformation. Calli selected by hygromycin were transferred to N6 medium containing 2 mg/L BAP, 30 g/L sucrose, 0.2 mg/L NAA, 250mg/l cefotaxime and 50mg/l hygromycin and several hygromycin resistant plants were obtained. Results : Stable incorporation of the introduced Apx8-35 to the genome of the hygromycin resistant plants was confirmed by PCR and Southern blot analysis. Transformation efficiency was variable between cultivates in which it was 13.5% in Potomac and 7.0% in Frontier. Conclusions : Constitutive expression of the transgene in the transformed orchardgrass tissues was identified by Northern blot analysis but transcript levels were different among individual plants.
Vasoconstriction Effect of Crotonis Fructus Extracts in rat thoracic aorta
Park, Wan-Su ; Kim, Hyeong-Hwan ; An, Deok-Gyun ; Choi Ho-Yeong ;
The Korea Journal of Herbology, volume 17, issue 2, 2002, Pages 167~167
Objectives : We have examined the vasoconstriction response to the various extracts of Crotonis Fructus (CF, Seed of Croton tiglium L.), defatted CF (DCF), roasted CF (RCF), and Croton oil in rat isolated thoracic aorta. Methods : We have examined the thoracic aorta segments obtained from rats immediately after delivery were mounted in organ baths superfused on a polygraph. Results : We found that the thoracic aorta segments responded to various extracts of CF with a dose-dependent vasoconstriction. In comparison to the 100 mM KCl induced contraction, it was contracted by 150% (water extract of CF), 144.5% (70% ethanol extract of CF), 80.1% (water extract of DCF) and 96.6% (ethanol extract of DCF) after addition of the 3 mg/ml each extract. The thoracic aorta segments were contracted by 118.5% (0.01 mg/ml Croton oil), 133.8% (3 mg/ml RCF for 10min), 123.1% (20min), 117.8% (30min), and 103.9% (40min). Conclusions : In the conclusion, CF, DCF, and RCF induced dose-dependent contraction in the isolated rat thoracic aorta. And it's major component, Croton oil, has potent vasoconstriction effect.
유통한약재 중 잔류농약에 관한 조사연구(Monitoring on Endocrine Disruptors in Natural Medicines Studies on Pesticide Residues in Natural Medicines)
Gang, In-Ho ; Jo, Jeong-Hui ; Kim, Do-Hun ; Sim, Yeong-Hun ; Kim, Eun-Gyeong ; Kim, Jong-Uk ; Hwang, Wan-Gyun ; Choi Ho-Yeong ;
The Korea Journal of Herbology, volume 17, issue 2, 2002, Pages 175~175
Objectives : Research on pesticide residues as endocrine disruptors in herbal medicines was initiated by Korea Food & Drug Administration in 1999. The presence and levels of certain pesticides in selected herbal medicines samples were determined. Forty species of herbal medicies which have no background data on pesticide residues including Osterici Koreani Radix were collected this year. Methods : 276 samples out of total 427 were circulated in Korea, and the others were in China. In order to analyze many pesticides in large number of samples, simultaneous multi-residue analysis of pesticides by GC-ECD was used. The GC-MSD analysis was followed to confirm the identity of the detected pesticide in each sample. Results and conclusions : The frequencies of pesticide detection in natural medicines were 18 samples circulated in Korea and 12 samples circulated in China. The range of pesticide residues for DDT was 0.007~0.09ppm in 8 samples. That for lindane was 0.001~0.02ppm in 7 samples, for endosulfan was 0.001~0.074ppm in 6 samples, for methoxychlor was 0.004~0.161ppm in 5 samples, for chlordane was 0.006~0.02ppm in 4 samples. The range of pesticide residues for parathion was 0.001ppm in 1 samples.
Inhibition effect of water extract of Cnidii Rhizoma on Angiotensin 2-induced angiogenesis
Mun, Seong-Il ; Kim, Hyeong-Hwan ; Jo, Jeong-Hui ; An, Deok-Gyun-Choe-Ho-Yeong ; Choi Ho-Yeong ;
The Korea Journal of Herbology, volume 17, issue 2, 2002, Pages 183~183
Objective : To estimate the angiogenic effect of water extract of Cnidii Rhizoma (ECR), we employed the rat sponge model for angiogenesis. Methods : It was based on subcutaneous implantation of sterile polyester sponges in the SD rat and we have characterized the dye method that incorporates 5% carmine red and 1% cresyl violet in 10% gelatin. vascularity index (V.I.) expressed as ㎍ dye/mg tissue. Subcutaneous implantation of sterile polyester sponges in SD rats enhanced the neovascularization over 14 days, as determined by measurements of relative sponge blood flow by a vascularity technique. The angiogenic response was verified by histological evaluation of vascularized sponges. Results : Daily oral administration of ECR (100 mg/kg for 14 days) produce no apparent effect on angiogenesis. The vasoconstrictor peptide, angiotensin Ⅱ (AⅡ), in daily doses of 100 nmol, caused an intense neovascularization but lower doses of AⅡ produced no apparent effect (1 nmol and 10 nmol). Co-administration of 100 nmol AⅡ and 100 mg/kg ECR p.o. depressed 100 nmol AⅡ-induced angiogenic effect. Conclusion : We have shown that p.o. daministration of 100 nmol AⅡ accelerated angiogenesis. Meanwhile, co-administration of 100 nmol AⅡ and 100 mg/kg ECR highly suppressed angiogenic property.
Studies on the Anti-oxidative effects of Tetragonia tetragonioides (Pall.) O. KUNTZE
Park, Yong-Gi ; Nam, Yu-Ho ;
The Korea Journal of Herbology, volume 17, issue 2, 2002, Pages 191~191
Objective : Tetragonia tetragonioides(Tt) has been used for injlammatory disease, skin disease and some kind of cancer in folk remedy. In order to investigate the effect of anti-oxidation of Tt, this study was done. Methods : Tt was investigated effects of antioxidation for DPPH(electron donating ability) radical ability, nitrate reductase(NR) activity, superoxide dismutase(SOD) activity, catalase activity, lipid peroxidase(LP) and glutathione(GSH). Results : In vitro, DPPH radical ability and nitrate reductase activity(NR) were significantly increased to the level of BHT. In vivo, compared with normal group, SOD activity and catalase activity were decreased in the acetaminophen treated group. On the other hand, Tt in the pretreated groups SOD activity and catalase activity were significantly increased. Compared with normal group, LP was increased in acetaminophen treated group. On the other hand, Tt in the pretreated groups were decreased. In the acetaminophen treated group, GSH activity was decreased by compare with normal group. And in the Tt pretreated groups, GSH activity was increased to the control group. Conclusion : According to the above results, the antioxidant anction of Tetragonia tetragonioides(Tt) is effective. And it is expected to be necessary to the study of the mechanism in the antioxidant of Tt.
Studies on the Anti-oxidative effects of Vespae Nidus
Park, Yong-Gi ;
The Korea Journal of Herbology, volume 17, issue 2, 2002, Pages 203~203
Objectives : Vespae Nidus(VN1) has been used for inflammatory disease, pain disease, skin disease and some kind of cancer in folk remedy. In order to investigate the effect of anti-oxidation of Vespae Nidus, this study was done. Methods : VN1 were investigated effects of antioxidation for DPPH(electron donating ability) radical ability, nitrate reductase(NR) activity, superoxide dismutase(SOD) sctivity, catalase activity, lipid peroxidase(LP) and glutathione(GSH). Results : In vitro, DPPH radical ability was not significantly increased to the level of BHT. But NR activity was significantly increased to the level of BHT. In vivo, compared with normal group, SOD activity and catalase activity were decreased in the acetaminophen treated group. On the other hand, VN1 in the pretreated groups SOD activity and catalase activity were significantly increased. Compared with normal group, lipid peroxidation was increased in acetaminophen treated group. On the other hand, VN1 in the pretreated groups were decreased. In the acetaminophen treated group, GSH activity was decreased by compare with normal group. And in the VN1 pretreated groups, GSH activity was increased to the control group. Conclusion : According to the above results, the antioxidant action of Vespae Nidus is effective. And it is expected to be necessary to the study of the mechanism in the antioxidant of Vespae Nidus
Effects of Bambusae Caulis in Liquamen Extracts on Histological and Haematological Change of the Liver and Kidney of Mice According to the Administered Dosage
Jeong, Jong-Gil ; Na, Chang-Su ; Kim, Gyeong-Su ; Hong, Gwang-Pyo ; Kim, Jeong-Sang ;
The Korea Journal of Herbology, volume 17, issue 2, 2002, Pages 213~213
Objectives : Bambusae Caulis in Liquamen extracts (BE) has been used for various curative effect that remove intense heat from the heart, replenish deficiency Yin, nourish deficiency blood, resolve phlegm. This study was performed to investigate the toxicity of BE to mice according to the administered dosage. Methods : The experimental groups divided four. Control group treated saline, J1 group treated BE 2and distilled water in the ratio of 1:15(0.05ml/kg), F2 group treated BE and distilled water in the ratio of 1:7(0.05ml/kg), J3 group treated BE and distilled water in the ratio of 1:17(0.05ml/kg). We administered BE to the mice for 24 weeks at interval of 48 hours, and investigated the body weight, changes in hematological profile, activities of the transaminase(GOT, GPT) and two hepatic antioxidant enzyme(SOD, catalase), and histological changes of the liver and kidney. Results : Leukocytes of J3 group were increased in the administered for 12 weeks, but decreased in the administered for 24 weeks. Platelets were decreased about 36% in J3 group treated for 12 weeks, but increased about 50% in the same group treated for 24 weeks. Blood total proteins were decreased slightly in the all experimental groups compared with control group. The activities of the transaminase and superoxide dismutase(SOD) were increased, but decreased activities of catalase in the J2 and J3 groups administered for 12 or 24 weeks. In the light microscopical observation, ballooned and injured hepatocytes in portal vein area of J1 group occurred, and dilated hepatocytes and disoriented sinusoid observed in J2 group administered extracts to mice for 24 weeks. Apoptosis occured in the ballooned and injured hepatocytes of the portal vein area in J3 group. A agglutination of RBC in glomerular capsule of the kidney observed in the J2 group, and destructed convoluted tubule observed in the J3 group treated for 24 weeks. Conclusions : These results indicate that mice treated with high dose BE develop a hematological changes, dysfuncton of the liver and kidney.
Effects of Sachungwhan and its component groups on Antioxidant Activities
Gwon, Yeong-Mo ; Park, Seon-Dong ;
The Korea Journal of Herbology, volume 17, issue 2, 2002, Pages 225~225
Objectives : Exposure to oxidant molecules can generate reactive oxigen species(ROS; for example, H2O2, O2-, OH
). These free radicals can alter DNA, proteins and membrane phospholipids. ROS are responsible for many diseases including cancer, neurodegerative diseases and cardiovascular diseases. Thus, to prevent against celluar damages associated with oxidative stress, antioxidants is important and many research about natioxidants are being conducted over the world. Methods : This study was performed to research the antioxidant activities of Sachunghwan(瀉靑丸) and its component groups in vitro. The experimental group was divided into 4 groups: Sachunghwan(SC), Samultang group(SC-1), Chungyul group(SC-2) and Haepyo group(SC-3). Results: 1. All experimental groups had significant antioxidant activity in linoleic acid system on concentration in comparison with control group. 2. In DPPH radical scavenging experiment and inhibitory effect experiment on superoxide generation in xanthine-xanthine oxidase system showed that all experimental groups had a dose-dependent effect. SC and SC-2 have more effect than SC-1 and SC-3. 3. Inhibitory effect experiment on superoxide generation induced by xanthine-xanthine oxidase system showed that all experimental groups had a dose-dependent effect. SC and SC-2 have more effect than SC-1 and SC-3. 4. In the inhibitory effect experiment on lipid peroxidation reaction in H2O2-Fe2+system showed that all experimental groups had a dose-dependent effect. SC-1 and SC-3 have more effect than SC and SC-2. 5. In the experiment on Nitrate scavenging activity showed that all experimental groups had a dose-dependent effect. SC and SC-2 have more effect than SC-1 and SC-3. Conclusion: Sachunghwan(SC) has an antioxidant activity and its antioxidant activity is likely to be associated with Chungyul group(SC-2).
유통 한약재의 중금속에 관한 연구(Studies on Monitoring Hazardous substances of Natural Medicines(Ⅰ) Studies on heavy metals of natural medicines in market)
Kim, Jong-Uk ; Choi Ho-Yeong ; Jo, Jeong-Hui ; Kim, Do-Hun ; Gang, In-Ho ; Sim, Yeong-Hun ; Kim, Eun-Gyeong ;
The Korea Journal of Herbology, volume 17, issue 2, 2002, Pages 235~235
Objectives : This study was performed to determine the harmful heavy metal ions(Pb, Hg, As, Cd, Cr) in twenty species of natural medicines circulated in Korea and China. Methods : The levels of heavy metal ions(Pb, As, Cd, Cr) were processed using kjeldahl digestion and ICP(Inductively Coupled Plasma spectometer). Mercury was determined by the amalgamation method. Results : Lead was detected less than 0.01(㎍/g) in most samples but in Achyranthes Root it was detected more than 1.0(㎍/g). The amount of Mercury was 0.01(㎍/g) in Trichosanthis Radix and 0.14(㎍/g) in Lonicera Flower. However, in Osterici Koreani Radix of Seong-do(China), Coix Seed of Jecheon(Korea) and Seoul(Korea) and Cimicifuga Rhizome of Seong-do(China), it was detected more than 1.2(㎍/g). The amount of Arsenic was determined 0.12(㎍/g) in Peach Kernel and 0.89(㎍/g) in Cimicifuga Rhizome. Cadmium was detected similar levels in most samples. Cadmium levels in Asiasarum Root was higher than any other samples and its value was 0.40(㎍/g). The amount of Chromium was detected 0.26(㎍/g) in Coix seed and 4.1(㎍/g) in Asiasarum Root. Chromium levels in Osterici Koreani Radix, Achyranthes Root and Asiasarum Root were higher than others. Hazardous heavy metals except Cr were detected less than 1.0(㎍/g) in most samples. Conclusion : The results mean that natural medicines were exposed to risk-hazardous environment.