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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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The Korea Journal of Herbology
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Journal DOI :
The Korea Association of Herbology
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Volume & Issues
Volume 18, Issue 4 - 00 2003
Volume 18, Issue 3 - 00 2003
Volume 18, Issue 2 - 00 2003
Volume 18, Issue 1 - 00 2003
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The Antioxidant Effect, Cell Viability and the Effect to the Gene Expression Using cDNA Microarray Assay of Shogunjungtang-gayounggolmorea
Kim Tae-Joong ; Lim He-Yong ; Kwak Min-Ah ; Byun Juun-Seok ; Seo Jung-Chul ; Han, Sang-Won ;
The Korea Journal of Herbology, volume 18, issue 2, 2003, Pages 1~1
Objectives : The purpose of this study was to determine the effect of SG-YM on antioxidant effect, cell viability and the expression of genes in human stomach cancer cells. Methods : Antioxidant effect of SG-YM was compared with that of vitamin C on DPPH radical, respectively. Cell viability was determiend by MTT assay. Finally, gene expression was evaluated by cDNA microarray assay. Results : 1. Scavenging effect of SG-YM on DPPH treated cells were demonstrated by differential antioxidant effect in SG-YM-treated group at the dose of 10 and lmg/ml. 2. Cell viability was increased in SG-YM-treated group at the dose of lmg/ml. 3. Among 8000 unigenes, 208 genes were upregulated while 90 genes were downregulated by SG-YM treatment on human stomach cancer cell line. Conclusions : Collectively, these results can be used as basic data for development of new anticancer drugs or treatment.
The Antioxidant Effect, Cell Viability and the Effect to the Gene Expression Using, cDNA Microarray Assay of Jengjengamiygin-tang
Lee Kwang-Soo ; Kim, Bong-Seok ; Kwak Min-Ah ; Byun Joon-Seok ; Seo Jung-Chul ; Han, Sang-Won ;
The Korea Journal of Herbology, volume 18, issue 2, 2003, Pages 13~13
Objectives : This study was undertaken to determine the effect of Jengjengamiygin-tang on antioxidant effect, cell viability and the expression of genes in human stomach cancer cells. Methods : Antioxidant effect of Jengiengamiygin-tang was compared with. that of vitamin C on DPPH radical, respectively. Cell viability was determiend by MTT assay. Finally, gene expression was evaluated by cDNA microarray assay. Results : 1. Scavenging effect of Jengjengamiygin-tang on DPPH treated cells were demonstrated by differential antioxidant effect in Jengiengamiygin-tang-treated group at the dose of Img/
. 2. Cell viability was increased in Jengiengamiygin-tang-treated group at the dose of 100 and l,
. 3. Among 8000 unigenes, 16 genes were upregulated while 389 genes were downregulated by Jengjengamiygin-tang treatment on human stomach cancer cell line. Conclusions : Collectively, these results can be used as basic data for development of new anticancer drugs or treatment.
The Processing Effects of Pyrite after Heating and Quenching in Vinegar
Hwang, Jeong ; Seo Young-Bae ;
The Korea Journal of Herbology, volume 18, issue 2, 2003, Pages 27~27
Objectives : To verify the processing effects of traditional operation include heating and quenching in vinegar, we studied phase changes and variation in concentration of poisionous metals caused by different conditions during processing of pyrite. Method : Pyrite was processed at temperatures up to
and through as many as 5 processing cycles. And toxic metal extraction test in water was carried out from the processed pyrites on the assumption that pyrite medicines with the lowest toxic metal content would be most desirable. Results : Increasing temperature and quenching in vinegar promoted phase change of pyrite to hematite, reduction of toxic metals in pyrite and their concentrations in the extraction solutions. The relationship between variations in extracted elements and the number of processing cycles at the same processing temperature were not clear. Conclusions : Traditional processing operations is very effective to reduce the toxicity of pyrite medicine, and it should be processed at the highest possible temperature, at least higher than
, in order to diminish highly toxic metals such as As, Pb. Co and Ni.
The Antimicrobial Activity of Cheongsangbangpungtang and its composition oriental medicines against Staphylococcus aureus
Jo Hee-Chang ; Oh Eun-Young ; Jee Seon-Young ; Seo, Bu-Il ;
The Korea Journal of Herbology, volume 18, issue 2, 2003, Pages 37~37
Objectives : Antimicrobial activities were assayed through the hot water extracts from Cheongsangbangpungtang and its composition oriental medicines. Methods : The author screened and isolated a Staphylococcus aureus from acne vulgaris patients. The optimum pH and temterature for the growth of the organism were in the range 7.0 and
, respectively. Results : Scutellariae Radix, Platycodi Radix, Coptidis Rhizoma, Gardeniae Fructus, Forsythiae Fructus, Menthae Herba, Angelicae Dahuricae Radix, Aurantii Fructus, Glycyrrhizae Radix that was more the size 10mm of inhibition zone among Cheongsangbangpungtang composition oriental medicines. Cheongsangbangpungtang showed inhibition zone of 14.29mm. Mic of extract from Cheongsangbangpungtang and its composition oriental medicines was 3-10
. Antimicrobial activity of the Cheongsangbangpungtang extract was not destroyed by the heating at
for 15min and not affected by pH. Conclusion : Cheongsangbangpungtang, Staphylococcus aureus
Effect of Chungajihwangtang on Osteoporosis in Ovariectomized Rats
Kim, Min-Su ; Seo, Bu-Il ; Kwak Min-Ah ; Ji Seon-Young ;
The Korea Journal of Herbology, volume 18, issue 2, 2003, Pages 49~49
Objectives : The present study has been undertaken to investigate the effects of Chungajihwangtang on osteoporosis in ovariectomized rats. Methods : In this experiment, the rats were ovariectomized. These rats were administered water extract of Chungajihwangtang. The levels of bone mineral density, ALP, calcium, phosphorus in serum, calcium, phosphorus, ash weight of bone, body weight and uterus weight were measured. Results : The bone mineral density of spinal bone, femoral bone and fibular-tibial bone was increased in comparison with those of OVX group in Chungajihwangtang treated group, and the change of BMD of spinal bone had statistical significance. The levels of serum phosphorus showed significant increase in comparison with those of OVX group at 8 weeks in Chungajihwangtang treated group. The levels of femoral and fibula-tibial calcium and phosphorus showed significant increase in comparison with those of OVX group in Chungqiihwangtang treated group. The levels of body weight showed decrease in comparison with those of OVX group in Chungajihwangtang treated group, but this result had not statistical significance. The levels of uterus weight showed increase in comparison with those of OVX group in Chungajihwangtang treated group, but this result had not statistical significance. Conclusions : Reviewing these experimetal results, it appears that Chungajihwang- tang was effective on osteoporosis. And further study on Chungajihwang-tang should be conducted to illustrate in depth the curing osteoporosis.
The Antioxidant Effect, cell viability and the effect to the gene expression using cDNA microarray assay of Opae-san
Han Sang-Soon ; Oh Jung-Han ; Kwak Min-Ah ; Byun Joon-Seok ; Seo Jung-Chul ; Han, Sang-Won ;
The Korea Journal of Herbology, volume 18, issue 2, 2003, Pages 59~59
Objectives : This study was undertaken to determine the effect of Opae-sun on antioxidant effect, cell viability and the expression of genes in human stomach cancer cells. Methods : In order to analyze the Antioxidant Effect, cell viability and the effect to the gene expression of Opae-sun, human stomach cancer cells were stimulated by Opae-san using cDNA microarray chip. After that, abstracted mRNA went through in situ hybridization and it demonstrated the effect to the gene expression. Results : 1. Scavenging effect of Opae-sun on DPPH radical treated with the DPPH radical did not demonstrated remarkably increased antioxidant effect in Opae-san treatment group. 2. MTT assay performed that more differentially increased cell viability in l00
Opae-san treatment group than contrast. 3. 47 gene significantly increased by Opae-san treatment. 4. 391 gene significantly decreased genes by Opae-sun treatment. Conclusions : Collectively, these results can be used as basic data for development of new anticancer drugs or treatment.
Double-blind Randomized Controlled Trials on Superficial Body Temperature of Korean Ginseng and American Ginseng in Koreans and Chineses
Seo Jung-Chul ; Lee Kyung-Lim ; Byun Joon-Seok ; Kim Myung-Soo ; Ha, Il-Do ; Heo Zheng-Jie ; Leem Kang-Hyun ; Han, Sang-Won ;
The Korea Journal of Herbology, volume 18, issue 2, 2003, Pages 71~71
Objective ; Generally it was known that Korean ginseng(Pm ginseng-white ginseng & red ginseng) increases body temperature, whereas Amencan ginseng(Pm quinquefolius) decreases body temperature. This study was designed to find out if Korean ginseng could increase body temperature comparing with American ginseng. Methods ; Double-blind randomized controlled trials on superficial body temperature of Korean ginseng and American ginseng in Koreans and Chineses was performed by using thermography at 10 points(Yintang(EX-HN3), Shuigou(GV 261, Danzhong(CV 17), Zhongwan(CV 12), Right Quanliao(SI 18), Left Quanliao(S1 18), Right RenyingGT 91, Left Renying(ST 9), Right Yunmen(LI 2) and Left Yunmen(LI 2). Results ; There was no significant difference between Korean ginseng and American ginseng in both Koreans and Chineses by repeated-measures ANOVA. Conclusion ; It was revealed that Korean ginseng does not increase superficial body temperature. Maybe the concept that Korean ginseng increase body temperature is concerned with commercial interests dealing with American Ginseng.
Experimental Studies on T-cell Activation of the Herba Lobeliae Chinensis
Seo Young-Bae ; Koo Bong-Gi ; Lee Yong-Koo ; Lee Young-Cheol ;
The Korea Journal of Herbology, volume 18, issue 2, 2003, Pages 87~87
Objectives This study was carried out for the purpose of knowing the effect from T-cell activation of the C57BL/6 mouse by the extraction from a extract of herba lobeliae chinensis(EHLC.). Methods We examined the influence of EHLC on T cell proliferation by using T cell gained in spleen and thymus gland of C57BW6 mouse. Also we examined cytotoxicity through SBR assay and immunocell through FACScan Results : The results were obtained as follows: 1. In order to know the effect of the cytotoxicity from extraction of EHLC, we had to examine the safe density of that on lung fibroblast cells of the mouse(mLFCs). EHLC did not show cytotoxicity against mLFCs. 2. In the proliferous effect of thymus T cells, water extracts of EHLC played an important role on the proliferating thymus T cells compared with control group at low density. 3. EHLC promoted the CW(B7-1) and Thl cytokines especially IL-2 gene expression, but decreased CTLA-4, IFN-
V, CD28 and Th2 cytokines IL-4 gene expression. 4. EHLC up-regulates
expression but not
by anti-CD3-stimulated T cells. Conclusions : above results indicates that EHLC play an important role in the activati on of autoreactive T cells by promoting Thl cytokine(I1-2) and dec reasing CTLA-4, IFN-Y, CD28 and Th2 cytokines II-4 gene expressi on, by up-regulating cell surface molecule(CD80, CD25, CD44). The refore further deep studies shoud be accomplished about its effects.
The Effect of Pear Pectin & Phenolic Compounds on Regional Cerebral Blood Flow, Mean Arterial Blood Pressure, Heart Rate and Cardiac Contractile Force in Hypertensive Rat Induced by 2K1C
Na, Chang-Su ; Yun, Dae-Hwan ; Choi Dong-Hee ; Jeong, Jong-Gil ; Eun, Jong-Bang ; Kim, Jeong-Sang ;
The Korea Journal of Herbology, volume 18, issue 2, 2003, Pages 101~101
Objectives : Oriental pear was used as treating disorder of heart and blood circulation in oriental medicine. This study was designed to investigate that pear pectin, pear phenolic compound effects on cardiovascular system as regional cerebral blood flow(rCBF), mean arterial blood pressure(MAP), heart rate, cardiac contractile force. Methods : The experiments were performed on Sprague Dawley rats. 2K1C hypertension model was prepared by constricting the left renal artery with a sliver clip. Apple pectin, pear pectin and pear phenolic compound solutions were administrated via femoral vein for Sweeks, a day's interval. Animals were divided into four groups, administration of apple pectin(AP1, pear pectin(PP) and pear phenolic compound( PPC ). Results : The results are that KBF was significantly increased at lmglkg in PPC group. The MAP was significantly decreased at l0㎍/kg in apple pectin, at l0
/kg in pear pecitn than before adminstration, but increased at 1 m p d k g in pear phenolic compound. There was no significant effects in heart rate. Cardiac contractile force was significantly increased a at l0
/kg, lmg/kg in pear phenolic compound. Conclusions : These results suggests that pear phenolic compound and pear pectin may be a significant effect in symptoms of heart and blood circulation..
The Antimicrobial Activity of Hyeonggaeyeongyotang and its composition oriental medicines against Klebsiella pneumoniae
Oh Eun-Young ; Jee Seon-Young ; Seo, Bu-Il ;
The Korea Journal of Herbology, volume 18, issue 2, 2003, Pages 109~109
Objectives : Antimicrobial activities were assayed through the hot water extracts from Hyeonggaeyeongyotang and its composition oriental medicines. Methods : The author screened and isolated a Klebsiella pneumoniae from rhinitis(or paranasal sinusitis) patients. The optimum pH and temperature for the growth of the organism were in the range 7.0 and
, respectively. Results : 1. The water extract of Hyemggaeyeongyotang, Angelime Gigantis Radix, Scutellariae Radix, Paem'ae Radix Alba, Ledebom'ellae Radix, Forsythiae Fructus, Cnidii Rhizoma, Glycyrrhizae Radix showed strong antimicrobial activities against Klebsiella pneumoniae. Size of inhibition zone(unit is mm) was Hyeonggaeyeongyotang 8.9, Angelime Gigmtis Radix 12.76, Scutellariae Radix 14.06, Paeomiae Radix Alba 11.28, Ledebouriellae Radix 11.21, Forsythiae Fructus 11.2, Cnidii Rhizoma 11.32, Glycyrrhizae Radix 11.47. 2. MIC of extract from Hyeonggaeyeongyotang and its composition oriental medicines was 3-5
. 3. Antimicrobial activity of the Hyeonggaeyeongyotang extract was not destroyed by the heating at 12 1 C for 15 min and not affected by pH. Conclusion : Hyeonggaeyemgyotang extract was expected it was able to be treated a pneumonia caused by Klebsiella pneumoniae
The Effect of Several Herbal Medicines which appear frequently as the effects of Hypoglycemia in Streptozotocin-induced Diabetic Rats
Choi Hae-Yun ; Jung Tae-Young ; Seo, Bu-Il ; Kim, Jong-Dae ; Park, Dong-Il ;
The Korea Journal of Herbology, volume 18, issue 2, 2003, Pages 121~121
Objectives : This study was aimed to verify the hypoglycemic effects of the herbs which appear frequently as the effects of antipyresis & putting out the fire(淸熱瀉火)-Phellodendri Cortex(黃柏), Scrophulariae Radix(玄蔘), Radicis Lycii Cortex(地骨皮), Gardeniae Fructus(梔子), Aloe(蘆회), Rehmanniae Radix(生地黃), Anemarrhenae Rhizoma(知母)-which were chosen among the herbs that were regarded as holding the hypoglycemic effects. Methods : To verify the hypoglycemic effects through the animal test, after the rats for test were treated 1 times with %mg/kg i.v. injection of streptozotodSigma, USA) which had been dissolved in the pH4.3 citrate buffer and were induced experimental hyperglycemia, the streptozotocin(ST2) induced hyperglycemic rats were prescribed with extract liquid 7.58 of each herb produced for test. And then the component such as glucose, insuline, and glucagon in serum and the change of body weight were measured and analyzed. Through that course, the significance for cure of DM were verified. Results : According to the booklet investigation, seven herbs which were useful for cure of DM were chosen and tested per 0s. As the result of that, It became evident that .Scrophulariae Radix, Anemarrhenae Rhizoma were significant for the hypoglycemic effects on DM. The insulin measurement showed the significant increase on both 3rd day and 7th day in the group of Scrophulariae Radix, Gardeniae Fructus, Aloe, Rehmanniae Radix and only showed the significant increase on 7th day ipl the group of Phellodendri Cortex, Radicis Lycii Cortex, Anemarrhenae Rhizoma. Besides, the glucagon measurement showed the significant decrease in the group of Radicis Lycii Cortex, Gardeniae Fructus, Rehmanniae Radix, Anemarrhenae Rhizoma. And the measurement of weight change showed the significant increase in all the sample herb groups. Conclusions : According to above result, Phellodendri Cortex, Scrophulariae Radix, Radicis Lycii Cortex, Gardeniae Frutus, Aloe, Rehmanniae Radix, Anemarrhenae Rhizoma were expected to be applied to the treatment of DM.
Effects of Benincasae Semen on Cultured Mouse Vascular Endotherial Cell Damaged by Glucose Oxidase
Seo, Eun-A ; Choi Seung-Suk ; Lee Byung-Chan ; Lee Jai-Kyoo ; Lee Kang-Chang ; Jeong, Jong-Gil ; Shin Min-Kyo ; Song Ho-Joon ;
The Korea Journal of Herbology, volume 18, issue 2, 2003, Pages 129~129
Objectives : It has been demonstrated that oxidative stress of oxygen radicals is involved in vascular disorder. The aim of this study was to evaluate the cytotoxicity of glucose oxidase (GO) and protective effect of herb extract on GO-induced cytotoxicity in the cultured vascular endotherial cell (VEC) of mouse. Methods : Cytotoxic effect of GO and protective effect of Benincasae Semen (BS) were performed by MTT assay, after cultured VEC were incubated in the media containing 1-30 mU/ml GO for 6 hours. Results : GO decreased cell viability in dose-and time-dependent mannner, and BS increased cell viability decreased by GO-induced cytotoxicity in these cultures. Conclusion : From above the results, GO has cytotoxic effect, and herb extract, BS is very effective in blocking GO-induced cytotoxicity in cultured VEC.
Effect of Aconiti Radix on Cultured Rat Myocardial Cells Damaged by Hydrogen Peroxide
Seo, Eun-A ; Lee Jai-Kyoo ; Lee Joung-Hwa ; Lee Byung-Chan ; Lee Kang-Chang ; Seo, Bu-Il ; Song Ho-Joon ;
The Korea Journal of Herbology, volume 18, issue 2, 2003, Pages 135~135
Objectives : To examine the cytotoxicity and the effect of Aconiti Radix(AR) on oxygen free radicals in cultured myocardial cells of neonatal rat. Methods : Toxic effect was measured by MTT assay after myocardial cells were incubated for 4 hours in the media containing 10-40 PM concentrations of H202. In addition, the protective effect of AR was determined in these cultures. Results : Cell viability was positively decreased in a dose-dependent manner after exposure of 30
to cultured rat myocardial cells for 4 hours. In the protective effect, AR prevented the
-induced cytotoxicity in these cultures. Conclusion : From these results, it suggests that
has cytotoxicity in cultured rat myocardial cells and AR blocked the cytotoxicity induced by
Characterization of the Polysaccharide extracted from the Fruit body and the Cultural Mycelia of Ganoderma lucidum IY005
Lee Jun-Woo ; Bang Kwang-Woong ; Seo, Bu-Il ; Lee Eun-Sook ;
The Korea Journal of Herbology, volume 18, issue 2, 2003, Pages 141~141
Objectives : To examine physico-chemical properties of the polysaccharide extracted from fruit body and mycelia of Ganodem lucidum IYOO5, which had been isolated from Kwangneung in Gyeonggi Province, Korea. Methods : Polysaccharide was extracted with hot water treatment and then purified with ethanol. We determined carbohydrate contents, composition of amino acids, infra-red spectrum and pattern of molecular weight. Results : Carbohydrate and protein contents of polysaccharide of fruit body was 49.5% and 37.7%, mycelia was 47.2% and 33.5%, respectively. The carbohydrate consisted of five monosaccharides and the protein contained 16 amino acids, and also molecular weights were 67 kD and 46 kD. Conclusion : The polysaccharide of fruit body and mycelia was shown an absorption band characteristic of the
-glycosidic linkage by infra red spectra. These results suggest that the polysaccharide extracted from fruit body and mycelia may similar properties with respect to physico-chemical.
Pharmacological Activities of the Polysaccharide obtained from the Fruit body and Mycelia of Ganoderma lucidum IY005
Lee June-Woo ; Bang Kwang-Woong ; Seo, Bu-Il ; Lee Eun-Sook ;
The Korea Journal of Herbology, volume 18, issue 2, 2003, Pages 149~149
Objectives : To examine pharmacological activity of Ganoderma lucidum IY005 which had been isolated from Kwangneung in Gyeonggi Province and extracted with hot water. Methods : We studied the pharmacoloical activity of polysaccharide from Ganoderma lucidum IY005. We measured antitumor activity against sarcoma 180 bearing mice, anticomplementary activity, activation of reticuloendothelial system, production of hemolytic plaque forming cell and nitric oxide production. Results : The tumor inhibition ratio of the polysaccharides obtained from fruit body and liquid cultured mycelia of Cmodenna lucidum IY005 against sarcoma 180 were 64.7% and 55.2%, respectively. And also, it was shown to activate reticuloendothelial system of ICR mice in the carbon clearance test and to increase hemolytic plaque forming cells of the spleen. Raw 264.7 macrophages treated with fruit body and mycelia fraction produced the nitric oxide and tumor necrosis factor-alpha. Conclusion : These results suggest that polysaccharides obtained from fruit body and mycelia of Ganoderma lucidum IY005 were similar to the immunological activities. And these fractions contain immunoregulatory components
Study on the regeneratory and inhibitory effect of anti-Alzheimer in pCT105-induced neuroblastoma cell lines by Ramulus et Uncus Uncariae water extract
Lee, Eun-Ju ; Park, Chi-Sang ; Park Chang-Gook ;
The Korea Journal of Herbology, volume 18, issue 2, 2003, Pages 157~157
Objectives : Many neurcdegenerative disorders and syndmmes are assodated with an excessive generation of reactive oxygen species (ROS) and oxidative stress. The pathways to nerve cell death induced by diverse potential neurotoxins such as peptides, excitatory amino acids commonly share oxidative downstseam processes, which can cause either an acute oxidative destruction or activate secondary events leading to apoptosis. Thus, A beta may not be the sole active fragment of beta-amyloid precursor protein (betaAPP) in the neurotoxicity associated with Alzheimer'diseaAAD). The pathophysiological role of CT105 derived APp695 has been intensively studied with CT105 expressing cell lines in vitro models of chronic neurodegenerative diseases such as AD. Methods : We focused on the amyloidegenic carboxyl terminal fragments of betaAPP containing the full length of A beta (CT105). We constructed a pCT105(carboxy-tenninal 105 amino acid fragment) of beta APP, examined the effects of CT105 in neuroblastoma cell lines. We established Ramulus et Uncus Uncariae water extract(RAM) against the CT105 and used them for the Western blot, RT-FCR. Tunel assay and immunccytochemishy analysis. Findings from our experiments have shown that RAM water extract may play an important role in protection and suppression against apoptosis induced by APP metabolic fragments (beta-amyloid [Abetal. carboxyl terminal [CT1051). which bas neumprotective activities in concentration-dependent (>15 micromoVd) and prevented CT(lG5-induced cytotoxicity in cultured neumblastoma cell lines. Also. RAM inhibited CT(105)binduced cytotoxicity when SK-N-SH cells was transfected with CT105. We have shown that RAM water extract inhibited and protect4 apoptotic effect by CT105, was shown to induce expression of cdc42, p38 MAF'K and extncellular signal-regulated kinases(ERK). in conbast expression of Caspase 3. iNOS and calpain by CT105 was suppressed when CT105 expression were incubated with RAM treabnent, which was confiied by RT-FCR and Western blot analysis. These results indicate that have also provided a molecular basis for the potential of using specific inhibitors for treating Alzheimer's disease (AD). Results : From these results, we proposed that RAM treabnent might improve the not only by suppressing apoptotic induction, but also by protecting CT(l05Finduced eurotoxicity probably through the increased expression of CT105 in neuroblastoma cells Conclusion : These observations suggest that RAM water extract may be alternative substances for the treatment of AD and may provide a potentially useful therapeutic strategy for advanced Alzheimer's disease(AD).
Effect of Water extract of Leonuri Herba on Vasospasm in a Rat Femoral Artery Model
Jung Yong-Bal ; Kim Hyung-Hwan ; Lee, Song-Deuk ; Ahn Duk-Kyun ; Shin Hye-Seung ; Baek Tae-Hyeun ; Choi Ho-Young ;
The Korea Journal of Herbology, volume 18, issue 2, 2003, Pages 169~169
Objective : In order to study the effect of water extract of Leonuri herba (ELS) on the subarachnoid hemorrhage animal model, the autologous whole blood was applied to the vicinity of the sprague dawley rat right femoral artery. Methods : Following periarterial application of autologous whole blood (PAB) and intraperitoneal injection of ELS (10, 100 and 300 mghg, P.o.), the vasomotor responses to ELS were identified and the histological changes, neovascularized blood vessel were observed. Results : Contractile response to ELS (10, 100 and 300 mg/kg, p.0.) was significantly decreased in the rat femoral arteries of PAB 5 and 7 days. In PAB 5 and 7 days, significantly increased vessel wall thickness and narrowed lumen were observed in comparison with the control group. In the arteries exposed to autologous whole blood for 5 and 7 days, a lot of newly generated capillaries in association with markedly curved blood vessel wall thickness, elastic lamina and irregularly oriented endothelial cells were observed in the periphery of the vessel wall. The number of neovascularized blood vessels were significantly decreased in the periphery of PAB with ELS (10, 100 and 300 mg/kg, p.0.) 5 and 7 days. In VEGF expression, Blood plus ELS (10, 100 and 300 mg/kg, p.0.)-exposed vessel were more weak expression than blood-exposed vessel to vein autologous whole blood for 5 and 7 days. Conclusions : From these results, we concluded that ELS inhibited posthemorrhagic chronic vasospasm and neovascularization in rat femoral artery model.
Effects of Acanthopanacis senticosi Radix on anti-CD40- and recombinant interleukin-4-induced cytokine production in murine B-cells
Sung Il-Chang ; Kim Hyung-Hwan ; Ahn Duk-Kyun ; Lee Yong-Sup ; Seo Young-Bae ; Choi Ho-Young ;
The Korea Journal of Herbology, volume 18, issue 2, 2003, Pages 179~179
Objectives : In order to study the anti-allegy effect of water extract of Acanthopanacis senticosi Radix (ASR) on the B-cells from healthy Balblc mice. Methods : The cytotoxicity of ASR was measured with the murine normal lung fibroblast cells by modified SRB assay. And the murine splenic B-cells was stimulated with anti-CD40 mAb and rIL4. The various cytokines related with allergy were measured by flow-cytometry and by RT-PCR with electophoresis. Results : The anti-allegy effects to ASR were identified and observed. The cytotoxicity of ASR on mouse lung fibroblast cells showed no significant activities. ASR had inhibitory effect on CD23+, CD69+, and IgE expression by ASR with anti-CD40 mAb plus rL-4-stimulated murine splenic B-cells. ASR had inhibitory effect on cytokines (E-l
b, IL-4, IL-6, IL-10, TNF-a, TGF-
) and transcript expression and IgE production by ASR with anti-CD40 mAb plus rIL-4-stimulated murine splenic B-cells. Conclusion : We concluded that ASR showed anti-allegy effect on murine splenic B-cells.
The Effect of Water Extract from Tripterygium regelii on Allergy
Byun Jong-Ho ; Kang Young-Sung ; Kim Sung-Soo ; Kim, Dae-Han ; Hwang, Dae-Ryong ; Shin Min-Kyo ; Song Ho-Joon ;
The Korea Journal of Herbology, volume 18, issue 2, 2003, Pages 189~189
Objective : To evaluate effect of allergy by Tripterygiwn regelii (TR), the biochemical changes associated with allergy were measured in BALF. serum splenocyte and lung of mice. Methods : After induction of allergic inflammation asthma by treatment of ovalbumine, water extracts from stem and leaf (SL) or flower (FL) of TR were taken orally for five successive days on mice, respectively. Result : In the total cell of BALF. the SL-TR extract (lCKhwk, h.w)-treated group was significantly reduced compared to control level and the reduction of eosinophil showed significantly at concentraton of 360 and loo0 dks, b.w. On the other hand, substantial decrease in IgE antibody formation was significantly observed by administration with SL-TR extract, whereas IgGZa was not affected. IgCl antibody formation appeared to be decreased inserum from SL-TR treated mice(l000 mg/kg, b.w). The content of IL-4 exhibited physiological role in splenocyte and lung was significantly reduced in the examined group compared to that of control after treatment of SL-TR extract(l@X/kg, b.w). In the case of IL-5, however, it was significantly reduced by FL-TR extract. In addition, IL-5 content in splenocyte was significantly reduced at concentration of 360 and loo0 d k g . b.w. by SL-TR dose, respxtively, and that of lung was significantly reduced dose-dewndently(l20, 360 and 1000mp/kg, b.w). Cnnclusion : It could be conduded that TripteTygiwn regelii showed beneficial effect on the allergic asthma and especially, its leaf and stem is more effective than flower.
Studies on the Allergic asthma effect of Semen Armeniaceae Amarum
Hwang, Dae-Ryong ; Kang Young-Sung ; Kim Sung-Soo ; Kim, Dae-Han ; Shin Min-Kyo ; Song Ho-Joon ;
The Korea Journal of Herbology, volume 18, issue 2, 2003, Pages 201~201
Objective : To evaluate the effect of Semen Armeniaceae Amarum (SAA) on allergy, studies were done experimentally. Methods : After induction of allergic inflammation asthma by treatment of ovalbumin, water extract from SAA were taken orally for five successive days on mice respectively. We were measured in BALF, serum IgE, FCA on the biochemical change associated with allergy. Results : In the total cell of BALF, the SAA extract 48Omg/kg treated group was significantly reduced compared to control group and the reduction of eosinophil showed significantly at concentration of 240, 48Omg/kg. The SAA extract (480mg/kg) group was significantly decreased compared to control group on IgE antibody formation. SAA dose-dependently also significantly inhibited to passive cutaneous anaphylaxis(PCA) activated by anti - dinitropheny 1 (DNP) IgE. Conclusions : These results indicate that Semen Armeniaceae Amarum showed benefical effect on the allergic asthma.