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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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The Korea Journal of Herbology
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Journal DOI :
The Korea Association of Herbology
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Volume & Issues
Volume 19, Issue 4 - 00 2004
Volume 19, Issue 3 - 00 2004
Volume 19, Issue 2 - 00 2004
Volume 19, Issue 1 - 00 2004
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Effects of Acorus Graminei Rhizoma Extract on Sociopsychological Stress in Mice
Cho, Su-In ; Kim, Hyung-Woo ; Jung, Yong-Sik ;
The Korea Journal of Herbology, volume 19, issue 4, 2004, Pages 1~1
Objectives : Stress is one of the deleterious factors for health, and many diseases are likely to be related to stress. The effects of Acorus Graminei Rhizoma extract were tested for the anti-sociopsychological stress action. Methods: 250g of dried Acorus Graminei Rhizoma was extracted(AGE) with pure water and the total extractive was evaporated under reduced pressure to give 39.7g. ICR male mice(
) were fed orally with the dose of l00mg/kg/day for five days. Mice were exposed to sociopsychological stress by restraining and seeing foot shock stressed mice for one hour for five days. Results: AGE administered group showed a significant decrease of serum corticosterone secretion compared with control group. Noradrenaline secretions in the dorsal cortex of brain showed no change. Lipid peroxidation of the liver and serum of mice were tested by measuring malondialdehyde, but AGE admistration showed no change. Conclusion: Above results suggest that AGE can rid the sociopsychological stress by decreasing serum corticosterone secretion.
Effect of Camelliae Japonicac Folium Extract on Regional Cerebral Blood Flow in Rats
Cho, Su-In ;
The Korea Journal of Herbology, volume 19, issue 4, 2004, Pages 9~9
Objectives: In brain disorders such as ischemic stroke, the final outcome depends largely on the duration and the degree of the ischemia as well as the susceptibility of various cell types in the affected brain region. In the present study, the effects of Camelliae Japonicae Folium extract(CJE) were tested for the anti-oxidative action in vitro and changes of MABP and rCBF in vivo. Methods : Anti-oxidative activity in vitro was measured by ranimat method, and changes of MABP and rCBF were determined by LDF methods. The rancimat method is commonly used to evaluate the antioxidative potency of various antioxidants and is based on the increase of electrical conductivity due to the formation of volatile dicarboxylic acids as a result of lipid oxidation. LDF allows for real time, noninvasive, continuous recordings of local CBF. The LDF method has been widely used to trace hemodynamic changes in the superficial or the deep brain structures in experimental stroke research. Results: MABP in CJE treated rats showed no significant change despite of the increase of CJE from 0.001 to 10 mg/kg(i.v.). CJE i.v. administration showed no change on MABP in normal rats. But CJE i.v. administration showed significant increase of rCBF as dose dependent manner. Propranolol pretreated MABP showed no significant change despite of the increase of CJE. rCBF of propranolol pretreated rats showed significant change from the i.v. injection concentration of 0.01 mg/kg. Conclusion : The ischemia/reperfusion induced oxidative stress may have contributed to cerebral damage in rats, and the present study provides clear evidence for the beneficial effect of CJE on ischemia induced brain injury. And the action mechanism of elevating effect of CJE on rCBF might not be concerned with the role of
. The action of CJE as an ROS-scavenger might underlie the mechanism. The exact component and mechanism remains for the future study.
Inhibitory Effects of Piperis Longi Fructus on the Accumulation of Eosinophils into Airways and Cell Proliferation
Lee, Young-Cheol ;
The Korea Journal of Herbology, volume 19, issue 4, 2004, Pages 17~17
Objective: Asthma is a chronic inflammatory disease of the mucosa and is associated with excess production of Th2 cytokines and eosinophil accumulation in lung. Piper longum L. is one of the well known herb used in oriental medicine for treatment asthma, bronchial and lung inflammation. In this study, effects of Piper longum L. water extracts on bronchoalveolar lavage fluids (BALF) cells proliferation, inflammatory cytokines production, and genes expression were evaluated. Anti-asthmatic effects of Piper longum L. in the development of OVA-induced airway inflammation and murine asthma model have not been fully investigated in vivo. To clarify the possible effects of Piper longum L. we examined the influence of Piperis longi fructus(PLF) on the development of pulmonary eosinophilic inflammation in a murine model of asthma. Methods: All mice were immunized on two different days (21 days and 7 days before inhalational exposure) by i.p. injections of 0.2 ml alum-precipitated Ag containing
of OVA bound to 4 mg of aluminum hydroxide in PBS. Seven days after the second sensitization, mice were exposed to aerosolized ovalbumin for 30 min/day on 3 days/week for 12 weeks(at a flow rate of 250 L/min,
ovalbumin in normal saline) and PLF (]50 mg/kg) were orally administered 3 times a week for 8 weeks. Results: The suppressive effects of PLF were demonstrated on the accumulation of eosinophills into airways, with reduction of eosinophil, total lung leukocytes numbers. Conclusions: These results indicate that PLF has a deep inhibitory effect on airway inflammation and hyperresponsiveness in murine asthma model and that suppression of reduction of eosinophil, total lung leukocytes numbers, eosinophil CCR3 expression and CD11b expression contributes this effect. Hence, the results indicated that PLF could act as a immunomodulator which possess anti-inflammatory and anti-asthmatic property.
Artificial Fruiting body of Paecilomyces japonica treated with dried-silkworm
Lee, June-Woo ; Bang, Kwang-Woong ; Seo, Bu-il ; Lee, Enu-Sook ;
The Korea Journal of Herbology, volume 19, issue 4, 2004, Pages 27~27
Objectives: This study was carried out investigate cultural conditions for artificial fruiting body production of Paecilomyces japonica. Methods: The formation period of an artificial fruiting body of Paecilomyces japonica treated with dried-silkworm, silkworm pupa and naked barley were 30 days and 35 days, respectively. Results: The length and diameter of fruiting body were longer and thicker in media of the naked barley than that of dried-silkworm. The optimum illumination was 200 lux for the fruiting body formation. Conclusion: The growth of fruiting body was not affected by three subsequent transculture, and the optimum inoculum size was six percent(w/v).
Identification of Korean Ginseng and American Ginseng by Using PCR-RFLP
Seo, Jung-Chul ; Seo, Bu-Il ; Han, Sang-Won ; Leem, Kang-Hyun ;
The Korea Journal of Herbology, volume 19, issue 4, 2004, Pages 35~35
Objective: This study was performed to determine if two ginseng species(Korean Ginseng and American Ginseng) could be identified by genetic analysis and to verify PCR(polymerase chain reaction)-RFLP(restriction fragments length polymorphism) analyses, which was used to assess genetic variation. Method: The DNAs of Panax species(Panax ginseng and Panax quinquefolius) were extracted, and we have investigated the typing single-base variations of Panax species in DNA by using PCR-RFLP. Result: The PCR-RFLP results showed clear data. American ginseng showed different pattern compared with Korean ginseng. Conclusion: PCR-RFLP might be able to provide the identification of the Panax species(Korean Ginseng and American Ginseng).
Identification of Akebia and Aristolochia specics by Pyrosequencing
Seo, Jung-Chul ; Seo, Bu-Il ; Han, Sang-Won ; Leem, Kang-Hyun ;
The Korea Journal of Herbology, volume 19, issue 4, 2004, Pages 39~39
Objective : Akebia species and Aristolochia species are some of the most medicinally important genera in the Oriental medicine. This study was performed to determine if Akebia species and Aristolochia species could be Identified by genetic analysis and to verify Pyrosequencing analyses, which was used to assess genetic variation. Method: The DNAs of Akebia quinnta Decaisne and Aristoluchia manshuriensis Kom. were extracted, and we have investigated the typing of single base variations of Akebia species and Aristolochia species in DNA by using Pyrosequencing. Result: Akebia quinata Decaisne showed different pattern compared with Aristolochia manshuriensis Kom. The peak of Akebia quinata Decaisne was very week in the second A nucleotide base but The peak of Aristolochia manshuriensis Kom. was very high. From these results we verified that our Akebia species and Aristolochia species-specific sequencing primers were well designed. Conclusion : Pyrosequencing analysis might be able to provide the identification of the Akebia species and Aristolochia species.
Genetical Identification of Korean Wild Ginseng and American Wild Ginseng by Using Pyrosequencing Method
Kwon, Ki-Rok ; Seo, Jung-Chul ;
The Korea Journal of Herbology, volume 19, issue 4, 2004, Pages 45~45
Objective: This study was performed to determine if two ginseng species(Korean wild ginseng. Panax ginseng and American wild ginseng, Panax quinquefolia) could be identified by genetic analysis and to verify Pyrosequencing analysis, which was used to assess genetic variation. Method: The DNAs of Panax species were extracted, and we have investigated the typing of single-base variations of Panax species in DNA by using Pyrosequencing. Result: The Pyrosequencing results showed clear data. American wild ginseng showed different pattern compared with Korean wild ginseng. The peak of American wild ginseng was very week in the first T nucleotide base but that of Korean wild ginseng was very high in the first T nucleotide base. Conclusion : Pyrosequencing analysis might be able to provide the identification of the wild Panax species(Korean wild Ginseng and American wild Ginseng).
The Antimicrobial Activity of Naesohwangryuntang and Its Composition Oriental Medicines
Chun, Seung-Chul ; Jee, Seon-Young ; Lee, Sang-Kon ;
The Korea Journal of Herbology, volume 19, issue 4, 2004, Pages 51~51
Objective : To evaluate the antimicrobial activities of Naesohwangryuntung and its composition oriental medicines against Streptococcus mutans, Candida albicans, Escherichia coli, Staphylococcus epidermidis, Staphylococcus aureus studies were done experimentally. Methods: Antimicrobial activities were assayed through the hot water extracts and the ethanol extracts from Naesohwangryuntang and its composition oriental medicines. The strains were incubated on culture medium and activated. We observed the size of inhibition zone on the strains that is incubated in strilized filter paper disc with various concentration extracts of Naesohwangryuntang and its composition. Results: The extracts of Naesohwangryuntang, Coptidis Rhizoma, Rhei Rhizoma, Paeoniae Radix, Gardeniae Fructus. Arecae Semen, Scutellariae Radix, Glycyrrhizae Radix showed antimicrobial activities against Staphylococcus aureus. The extracts of Naesohwangryuntang, Coptidis Rhizoma, Forsythiae Fructus, Rhei Rhizoma, Paeoniae Radix, Gardeniae Fructus, Arecae Semen, Scutellariae Radix, Glycyrrhizae Radix showed antimicrobial activities against Staphylococcus epidermidis. The water extracts of Naesohwangryuntang, Rhei Rhizoma, Paeoniae Radix, Coptidis Rhizoma, Scutellariae Radix, Glycyrrhizae Radix showed antimicrobial activities against Streptococcus mutans. The extracts of Coptidis Rhizoma showed antimicrobial activities against Candida albicans. All of the extracts showed no antimicrobial activities against Escherichia coli. Conclusion: We observed antimicrobial activities of Naesohwangryuntang and its composition against Streptococcus mutans, Staphylococcus epidermidis, Staphylococcus aureus.
Determination Methods of Citrus unshii Percarpium by HPLC
Cho, Sung-One ; Homg, Seon-Pyo ; Choi, Ho-Young ; Choi, Hwan-Soo ; Lee, Je-Hyun ;
The Korea Journal of Herbology, volume 19, issue 4, 2004, Pages 61~61
Objective: Citrus unshiu Markovich(Rutaceae) has been used in traditional medicine as a digestant, expectorant, and in the treatment of diarrhea, anal prolapes. Hesperidin, naringin contents of citrus fruits have been determined. Methods: The Citrus unshill was dried in dry oven. The extraction was sonicated in
methanol in respectively. A quantitative analysis of hesperidin, naringin using HPLC method. Analysis of naringin, hesperidin, neohesperidin, narigenin and hesperetin was conducted by HPLC with reversed-phase C18 column and a UV dctecter at 204 nm. Elution was carried out at 1.0 ml/min with
methanol. Result and Conclusion : Sonication in
methanol for 1.5hr was the better extracting method that can extracted naringin and hesperidin at the same time. And
methanol of mobile phase was good resolution and could analyze into naringin and hesperidin at the same time.
Effect of Joagumhwan and its components on acetaminophen induced hepatoxicity in rats
Choi, Oi-Chul ; Lai, Yung-Sheng ; Park, Sun-Dong ;
The Korea Journal of Herbology, volume 19, issue 4, 2004, Pages 67~67
Objective : Acetaminophen, which causes acute liver injury in humans and animals, has made useful inducer of hepatoxicity for studying hepatopreventive drugs. Juagumhwan is known as one of the hepatopreventive drugs. However, its mechanism of recovery of hepatoxicity treated with acetaminophen is poorly understood, this study was performed to observe the antioxidative effect of Joagumhwan extract and its several combination group. Methods: This study was performed to research the antioxidant activities and hepatoxicity of Joagumhwan extract and its several combination groups, The experimental group was divided into 3groups : Joagumhwan Ⅰ (JGH Ⅰ), Joagumhwan Ⅱ (JGH Ⅱ), Joagumhwan Ⅲ (JGH Ⅲ). Results: KGH Ⅰ reduced the amount of lipid peroxide in serum and liver by inducing antioxidative enzymes such as catalase, and increased the amount of glutathion-s-transferase in vitro in vivo. Morever, JGH Ⅲ also had antioxidative effect in vivo. JGH I showed significant effect on recovery of hepatoxicity (GOT. GPT,
, ALP, LDH, bilirubin) and cell survival. Conclusion: Therfore, these results suggest that JGH I is very useful therapatic agest for recovery of hepatoxicity by acetaminophen overdose.
Effects of In Vivo Synergism of Mawhangyounpye-tang, a Poly-Herbal Formula and Rufloxacin of Quinolones against Klebsiella pneumoniae Respiratory Infections
Seo, Jeng-Yim ; Jeon, Kwi-Ok ; Song, Kwang-Kyu ; Park, Mee-Yeon ; Choi, Hae-Yun ; Kim Jong Dae ;
The Korea Journal of Herbology, volume 19, issue 4, 2004, Pages 81~81
Objectives & Methods : In order to evaluate the in vivo synergic effect of Mawhangyounpye-tang which was a traditional poly-herbal formula has been used in the treatment of respiratory diseases in Korea, with quinolone antibiotic, rufloxacin (RUFX), the viable bacterial number and histopathological changes were monitored after experimental ,respiratory infection with Klebsiella peumoniae NCTC 9632. Results: The obtained results were as follows: 1. In RUFX group, the viable bacterial numbers were significantly decreased compared to that of control group and these were more dramatically decreased compared to that of single treatment with RUFX, respectively in concomitant treated groups with Mawhangyounpye tang. 2. In control group, severe infiltration of inflammatory cells, hemorrhage and hypertrophy of alveolar linings were demonstrated at microscopical levels. However, these abnormal histopathological changes were significantly decreased compared to that of control group in RUFX group, and these were more dramatically decreased compared to that of single treatment with RUFX, respectively in concomitant treated groups with Mawhangyounpye-tang. 3. In RUFX group, the LSA (luminal surface of alveoli
) were significantly increased compared to that of control group and these were more dramatically decreased compared to that of single treatment with RUFX, respectively in concomitant treated groups with Mawhangyounpye-tang. Conclusion: According to these results, it is considered that in vivo antibacterial activity of RUFX was dramatically increased by concomitant use of Mawhangyounpye-tang against Kebsiella pneumoniae NCTC 9632 infection of respiratory tract.
The preventive effect of Bupleuri radix on liver damage induced by acetaminophen in the rats
Kweon, Young-Mo ; Lee, Jea-Eun ; Park, Sun-Dong ;
The Korea Journal of Herbology, volume 19, issue 4, 2004, Pages 91~91
Objective : Acetaminophen is a widely used analgesic and antipyretic drug throughout the world. It is also an over-the-counter drug and considered a safe drug. But its overdose and other causes such as alcoholism and diabetes can bring about hepatotoxicity. The hepatotoxicity of APAP is believed to be due to NAPQI proouced by CYPs and reactive oxygen species (ROS). Method: Bupleuri radix(BR) at 500 mg/kg (p.o.) was administered to the male Sprague Dawley (5 weeks old)rats for one week, and then APAP at 500 mg/kg (abdominal injection) was given 24h after BR administration. This study was performed to research the protective effects of BR extract against APAP-induced hepatotoxicity. Result: The data including GOT, GPT, histological examination and GST indicated that BR has a significant antidotal effect on APAP overdose. Also, the date including LPO and catalase showed that BR has a antioxidant effect which is one of mechanisms of the hepatoprotective effect of BR. Finally, the data including GSH, GST, RT-PCH, Western blot showed that BR has a suppressive effect on the production of NAPQI, by regulating the expression of CYP 2EI. Conclusion: Based on these data, we concluded that the mechanisms underlying the protective effect of BR against APAP-induced liver damage are both the antioxidant effect against ROS and the inhibitory effect on the expression of CYP 2El, which leads to the suppression of NAPQI production.
Studies on the Effects of Gle-PPT and PPT, Intestinal Metabolites of Ginsenoside, on the Immune System
Kim, Dae-Han ; Song, Ho-Joon ; Jeong, Jong-Gil ; Sim, Young-Seop ; Shin, Min-Kyo ;
The Korea Journal of Herbology, volume 19, issue 4, 2004, Pages 105~105
Objectives : Ginsenosidcs from Panax ginseng are metabolized by human intestinal bacteria after oral administration of ginseng extract. 20-0-(
)-20(S)-protopanaxadiol (Glc PPD) and 20(S)-Protopanaxatriol (PPT) are reported as the major intestinal metabolites of ginsenosides. However, the pharmaco-logical effects of Glc-PPD and PPT are not yet evaluated. The aim of this study, therefore, is to investigate whether Glc-PPD and PPT could affect the releases of interferon-
and interlcukin 10 (IL-10), two important cytokines which can regulate immune function, by activated T cells and the productions of nitric oxide (NO) and prostaglandin E2 (PGE2), two effector molecules that mediate inflammatory processes, by activated macrophages. Methods: Cytokines produced by human jurkat T cells activated with concanavalin A were measured by enzyme-linked immunosorbent assay (ELISA). No and PGE2 produced by murine RAW 264.7 macrophage activated with lipopolysaccharide were measured by Griess reagent and ELISA, respectively. Results: PPT reduced dose-dependently
and IL-10 releases by T cells stimulated with the mitogen concanavalin A. PPT also inhibited NO and PGE2 productions by RAW 264.7 cells stimulated with the endotoxin lipopolysaccharide. Unlike PPT, Glc-PPD had no effect on either T cell-mediated cytokine release or macrophage mediated effector molecule production. Conclusion: These results suggest that PPT may be developed as a useful agent for regulating immune responses and curing inflammatory diseases.
The effect of Radix Seutellariac Water Extract on the asthma induced by Ovalbumin
Hwang, Dae-Young ; Lee, Seong-Cheol ; Shin, Ho-Dong ; Shin, Min-Kyo ; Kim, Jae-Hong ; Jeong, Jong-Gil ; Song, Ho-Joon ;
The Korea Journal of Herbology, volume 19, issue 4, 2004, Pages 117~117
Objectives: To evaluate effect of allergy by Radix Scutellariae Water Extract(RS), the biochemical changes associated with allergy were measured in Broncho Alveola Lavage Fluid(BALF), serum, splenocyte and lung of mice. Methods: After induction of allergic inflammation asthma by treatment of Ovalbumine(OVA), RS were taken orally for five successive days on mice, respectively. Results: In the total cell of BALF, the RS(l000mg/kg) treated group was significantly reduced compared to control level, and the reduction of eosinophil showed significantly at concentration of RS(300, 1000 mg/kg). Substantial decrease IgE antibody formation was significantly observed by administration with RS(300, l000mg/kg), whereas IgGI, IgG2a antibody formation was not affected in serum from RS treated mice. The content of IL-4 exhibited physiological role in lung, splenocyte was not significantly reduced compared to control group after treatment of RS, but I-5 was significantly reduced at concentration of RS (300, l000mg/kg). Conclusions : From the above result, it could be concluded that Radix Scutellariae Water Extract showed beneficial effect on the allergic asthma.
Isolation of antimicrobial compound against Klebsiella pneumonia from Hyeongbangjiwhangtang
Jeong, Jong-Hyo ; Seo, Eun-Hee ; Park, Sunk-Joon ; Goo, Deok-Mo ; Lim, Byung-Mook ; Kim, Young-Ju ; Kang, Hyun-Gu ; Seu, Young-Bae ;
The Korea Journal of Herbology, volume 19, issue 4, 2004, Pages 127~127
Objectives: Klebsiella pneumoniae(K. pneumoniae)is a pathogen that cause respiratory infection such as pneumonia, empyema and otitis in childrens, old man or patients after operation. In this study, we were assayed a new antimicrobial material through the hot water extracted from Hyeongbangjihwangtang against Klebsiella pneumoniae that isolated from rhinitis patients. Methods: The raw materials of Hyeongbangjihwangtang were added and boiled down. After 2hours it was enriched at 1ℓ and extracted with a solvent. And then, we measured its antimicrobial activities and studied another specific characters. To isolate the antibiotic materials for K. pneumoniae, the aqueous boiled Hyeongbangjihwangtang was extracted with various organic solvents. The antibiotic materials were well extracted in ethyl acetate and it has pale yellow color. We isolated the antimicrobial compound HJT-4 from the ethyl acetate extract of Hyeongbangjihwangtang after chromatographic separation. Result: The purified antibiotic material, HJT-4, was not originated in raw materials of Hyeongbangjihwangtang but it was a different one. The MIC of HJT-4 was
. Conclusion : As above, the HJT-4 from Hyeonggaeyeongyotang has high antimicrobial activities against Klebsiella pneumoniae. This results suggest that it will be able to be treated not only a pneumonia caused by Klebsiella pneumoniae but also many other respiratory diseases.
An Experimental study on the Synergic Effect on Jawoongo and Gentamicin in Dermal Wound Healing and Skin Wound Infection against Staphylococcus aureus, Streptococcus pyogenes, and Pseudomonas aeruginosa
Kim, Su-Kyung ; Choi, Hae-Yun ; Jee, Seon-Young ; Lee, Sang-Kon ;
The Korea Journal of Herbology, volume 19, issue 4, 2004, Pages 137~137
Objectives : The purpose of this study is to evaluate the synergic effect of Jawoongo(紫雲膏) with gentamicin. Methods: The minimal inhibitory concentration(MIC), MIC50 and MIC90 of single use of gentamicin and Jawoongo with their concomitant treatment against 3 strains of bacteria, Staphylococcus aureus(S. aureus), Streptococcus pyogenes( S. pyogenes), Pseudomonas aeruginosa(P. aeruginosa) were observed. In addition, changes of wound size(CT50), viable bacterial numbers, total white blood cell(WBC) count and WBC differential count were also monitored after experimental excision skin wound infection mouse models with S. aureus ATCC 25923, S pyogens NCTC 10662 and P. aeruginosa ATCC 8668. Result : 1. In concomitant treatment of gentamicin and Jawoongo, relatively favorable antibacterial effects were Jawoongo-dose dependently demonstrated against three strains. 2. In concomitant treatment of gentamicin and Jawoongo, relatively favorable decrease of wound size were Jawoongo-dose-dependently demonstrated. 3. In concomitant treatment of gentamicin and Jawoongo, relatively decrease of CT50 was Jawoongo dose-dependently demonstrated in all tested models. 4. In concomitant treatment of gentamicin and Jawoongo, significantly decrease of decrease of WBC numbers neutrophil and monocytes, and increase of lymphocytes were Jawoongo-dose -dependently demonstrated in all tested models. 5. In concomitant treatment of gentamicin and Jawoongo, significantly decrease of viable bacterial numbers were Jawoongo-dose-dependently demonstrated in all tested models. Conclusion: Based on the above results, in concomitant treatment of gentamicin and Jawoongo, dual effect to antibacterial activities and accelerating effect of wound healing were demonstrated with synergic effect.
Effect of Hwangryunhaedok-tang and its modified preseription on the recovery of spatial cognitive function in the brain ischemia induced by four-vessel occlusion in rats
Kong, Min-Jung ; Ha, Ni-Na ; Lee, Ha-Young ; Kim, Yong-Tae ; Rho, Seung-Ju ; Kim, Ho-Cheol ;
The Korea Journal of Herbology, volume 19, issue 4, 2004, Pages 161~161
Objectives : The purpose of the study we report here was to determine recovery of spatial cognitive of HwangRyunHaeDok-Tang (Huang-lian-jie-du-tang, HT, 黃連解毒湯) and its modified prescription(HTM) on global ischemia induced by 4-vessel occlusion in wistar rats. Method : The sample of HT and HTM were prepared by lyophilization after extraction with
water. Rats were induced to 10 minutes of forebrain ischemia by 4-vessel occlusion (4-VO) and reperfused again. They were administered at a dose of 300mg/kg to 0 minutes and 90 minutes after 4-VO. Functional improvement was evaluated with the Morris water maze at 7 days after ischemia. Rats were sacrificed after behavior test and the number of CAl pyramidal neurons were counted in hippocampal sections stained with cresyl violet. Result: Compared to control group, HT and HTM groups confimed that contributes to behavioral improvement. The administration of HT and HTM showed a significant neuroprotective effect on hippocampal CAl neurons after ischemia compared to cotrol group. Conclusion : The results of this study demonstrate that administration of HT and HTM is effective in reducing neuronal damage and functional recovery in response to transient forebrain ischemia.
Comparison with Essential oils of Angelicae koreanae Radix
Choi, Ho-Young ; Sub, Young-Bae ; Ham, In-Hye ;
The Korea Journal of Herbology, volume 19, issue 4, 2004, Pages 169~169
Objective : Gangwhal (羌活) is the root of Angelica koreana Kitagawa (family Umbelliferae) and a popular crude drug used to expel wind from the body surface ( 風解表), to remove dampness ( 風濕) and to relieve pain (止痛). There are two kinds of Gangwhal which are NamGangwhal(As) and BukGangwha(An) according to cultivated methods and ecotype. Also, fresh one(Ds) and dried one(Dn) were comparison of essential oils contents. Methods : GC/MS method was used for essential oil analysis and tried to distinct of two types of Gangwhal. Result: 1. In the comparison with essential oils of As and An, sabinene was more 11.24 times in As than An and cresole was 2.03 times in An more than As. Terpinolene and osthole was detected only in As. 2. Ingredient analysis of essential oils of Ds and Dn, comparison of fresh gangwhal with dried gangwhal, there is no big change in kind of essential oils, but change appeared for amount of ingredient. In case of sabinene, it was more 2.63 times in Ds more than Dn, and in case of 4-vinyl-2-methyl acetophenone, it was more 4.26 times in Dn more than Ds. Osthole was detected in Ds but wasn't detected in Dn. 3. Osthole is detected in only fresh and dried NamGangwhal. Conclusion: Osthole is one of distinction compound of NamGangwhal and BukGangwhal.
Two Cases Report of Pneumonia through Treatment of Cheongpyebyulgap-san(淸肺鱉甲散)
Song, Kwang-Kyu ; Seo, Young-Ho ; Jeon, Kwi-Ok ; Choi, Hae-Yun ; Kim, Jong-Dae ;
The Korea Journal of Herbology, volume 19, issue 4, 2004, Pages 179~179
Two patients were dignosed as pneumonia through simple chest X-ray. They were suffering from cough, sputum, fever. chilling sign etc. We performed oriental medical treatment like herbal medicine without using western antibiotics. Through those treatments we could get good results. The herb medicine used at this case was mainly Cheongpyebyulgap-san(淸肺鱉甲散). From the results, we could find out that the oriental medical treatments were very effective to reduce inflammatory fever and to care pneumonia. So in order to prevent antibiotics abuse, we have to make more researches into pneumonia, and try to find out more effctive oriental medical therapies for infectious diseases.
For Improvement of Administration System of Herbal Medicine as Authorized Drugs
Park, Seong Kyu ; Kim, Chung-Sook ; Cui, Xun ; Kim, Yun-Kyung ;
The Korea Journal of Herbology, volume 19, issue 4, 2004, Pages 187~187
Objective: This study is to explore ways toward improvement of administration system of herbal medicine. Though herbal medicine have been used as traditional therapeutic drugs for a long time. But in these modern days to improve people's health, the government authorities should not only ensure safety and efficacy, but also guarantee quality of herbal medicine as authorized drugs. Methods : We analyzed the present provisions of the law and regulations and reviewed preceding researches. We also exchange opinions and take some advice of related experts. Results and Conclusions : For more effective handling of raw herbs, the exact defining of raw herb and herbal medicine at law is preferential. Marketing structure should be devided into agricultural products and standardized drugs. Professional education and expert training is needed in herb cultivation and distribution control. The existing standardized herb system and quality control should be strengthened and act in line with raw herb's charicteristics. Oriental pharmacist should carry out supervision of the quality control and standized herb system as experts.
Study on one Bangchonbi of Shanghanlun
Kim, In-Rak ; Kim, Kwang-Duk ;
The Korea Journal of Herbology, volume 19, issue 4, 2004, Pages 195~195
Objectives : The purpose of this study was to determine new assumptive value of one Bangchonbi of Jung-Kyung fomulae. Methods: Find out the Bangchonbi that the one spoon of Jangjunggyung period. and measured use Bangchonbi. And find out the new assumptive vaule. Results: 1) The 1 Bangchonbi is 2.0mL. 2) The 1 Bangchonbi of Banhasan is l.lg, Sayuksan is l.0g. Orungsan is 0.8g. 3) The 1 Bangchonbi of jeoksukji power is l.8g, munhabsan power is 2.5g. 4) The 1 Bangchonbi of moryutaeksasan is l.0g. 5) The 1 Bangchonbi of sogonsan is 0.19g. Conclusions : According to the results mentioned above, the assumptive value of 1 Bangchonbi is 2.0mL.