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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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The Korea Journal of Herbology
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Journal DOI :
The Korea Association of Herbology
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Volume & Issues
Volume 21, Issue 4 - Dec 2006
Volume 21, Issue 3 - Sep 2006
Volume 21, Issue 2 - Jun 2006
Volume 21, Issue 1 - Mar 2006
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Tyronase Inhibitory Effect of 3,4-Dihydroxybenzaldehyde Isolated from Pinellia ternata
Shin, Dong-Soo ; Paik, Do-Hyeon ; Yoon, Do-Young ; Shin, Dong-Min ; Cho, Yong-Kweon ;
The Korea Journal of Herbology, volume 21, issue 2, 2006, Pages 1~7
Objectives : The purpose of this study is to isolate tyrosinase inhibitory material from Pinellia ternata and characterize its own structure and activity. Methods : Pinellia ternata (600g) was extracted with 95% methanol (1L) at
for 4 days, with shaking at 250rpm. The extract was further solvent-fractionated with n-hexane, chloroform, ethylacetate and water. The active fraction was subjected to JAI recycling prep-HPLC JAIGEL GS-320 column. The structure was identified for the active peak with NMR and GC. Results : Tyrosinase was potently inhibited by 95% methanol extracts from Pinellia ternata. The
value of the extracts was estimated to be 0.05mg/ml. The extracts was divided into four solvent-fractions, and the most potent tyrosinase inhibition was found in ethylacetate layer.
value of ethylacetate fraction was 0.001mg/ml. This fraction was further purified with JAI Recycling Preparative HPLC (Model: LC 9104). The isolated compound showing inhibitory activity was characterized on its chemical structure by NMR and the compound was identified as 3,4-dihydroxybenzaldehyde.
was found to be 7.74
which is much lower than that of kojic acid
. Conclusions : The data suggest that 3,4-dihydroxybenzaldehyde isolated and identified from Pinellia ternata is very strong inhibitor to melanin biosynthesis.
Determination of the DNA Sequence of the 18S rRNA Gene of the Rehmannia glutinosa and Its Phylogenetic Analysis
Bae, Rebecca E. ; Shin, Dong-Min ; Bae, Young-Min ;
The Korea Journal of Herbology, volume 21, issue 2, 2006, Pages 9~13
Objectives : To determine the DNA sequence of the 18S rRNA gene of the Rehmannia glutinosa and analyze it phylogenetically Methods : Dried root of the Rehmannia glutinosa was ground with a mortar and pestle. Glass beads(0.5 mm in diameter), TE buffer and SDS solution were added to that. The mixture was vortexed vigorously and extracted with the mixture of phenol, chloroform and isoamyl alcohol and with the mixture of the chloroform and isoamyl alcohol. The nucleic acids were precipitated with ethanol and resuspended in TE buffer. Contaminating RNA was digested with RNAse A and the DNA was purified further with the Geneclean Turbo Kit. This DNA was used as a template for amplification of the 18S rRNA gene by PCR. The PCR product was cloned in the pBluescript SK II plasmid by blunt-end ligation and the DNA sequence of the insert was determined. This DNA sequence was analyzed phylogenetically by the BLAST program. Results and Conclusion : Vortexing the ground powder of the dried plant root with glass beads during cell lysis improved recovery of DNA. The DNA sequence of the Rehmannia glutinosa 18S rRNA gene was determined and deposited at the GenBank as the accession number DQ469606. Phylogenetic analysis of that sequence showed the relationship between the members of the family of Scrophulariaceae and also the close relationship of the Buddleja davidii to the members of the Scrophulariaceae family.
A Herbological study on the plants of Gymnospermae in Korea
An, Sang-Ho ; Jeong, Jong-Kil ;
The Korea Journal of Herbology, volume 21, issue 2, 2006, Pages 15~26
Objectives : The plans can be used for medicinal purposes among Gymnospermae in korea and examined their effects and distributions. Methods : The examined herbalogical books and research papers which published at home and abroad. Results : 1. Pinus genus is main kind enough that it has 22 species among 75 species in the Gymnospermae of which medicinal plants are 7 species. 2. Folium is the main medicinal parts of medicinal plants in the Gymnospermae which is used in 22 genera. 3. According to nature and flavor of medicinal plants in the Gymnospermae, they were classified into warm 19 genera, balance 18 ; bitter taste 25, sweet taste 21. 4. According to meridian propism of medicinal plants in the Gymnospermae, they were classified into spleen meridian 12 genera, lung meridian 11, liver meridian 9, heart meridian 7. 5. According to the properties and principal curative action, they were classified into drugs for relieve wind and moisture 37 genera, drugs for relieve pain 21, drugs for hemostasis 15, drugs to activity 14. 6. It was researched that toxic medicinal plants include toxin were 8 kinds. Conclusion : There were totaled to 21 genera and 75 species in Gymnospermae in Korea and among them medicinal plants are 14 genera, 28 species, some 37% in total.
A Herbological study on the plants of Fagales in Korea
Kim, Bong-Kyoo ; Jeong, Jong-Kil ;
The Korea Journal of Herbology, volume 21, issue 2, 2006, Pages 27~36
Objectives : The plans can be used for medicinal purposes among Fagales in korea and examined their effects and distributions. Methods : The examined herbalogical books and research papers which published at home and abroad. Results : 1. Quercus genus is main kind enough that it has 32 species among 94 species in the Fagales, of which medicinal plants are 8 species. 2. Cortex is the main medicinal parts of medicinal plants in the Fagales which is used in 12 species. 3. According to nature and flavor of medicinal plants in the Fagales, they were classified into balance 23 species, cool 8 and warm 6; rough taste 25, bitter taste 21 and sweet taste 10 in the order. 4. According to meridian propism of medicinal plants in the Fagales, they were classified into large intestine meridian 11 species, spleen meridian 9, lung meridian 8, liver meridian 8 and stomach meridian 6 in the order. 5. According to the properties and principal curative action, they were classified into drugs for antifebrile and detoxicant 16 species, drugs for hemostasis 14, drugs for astriction 11, drugs to relieve cough 8 in the order. 6. It was noticed that every medicinal plant in the Fagales is nontoxic. Conclusion : There were totaled to 9 genera and 94 species in Fagales in Korea and among them medicinal plants are 6 genera, 22 species, some 23% in total.
Effect of Dangguibohyultang and its combinations on apoptosis in human colorectal adenocarcinoma HCT116 cells
Kim, Byung-Wan ; Yun, Hyun-Joung ; Jeon, Hyeon-Suk ; Yun, Hyung-Joong ; Kim, Chang-Hyun ; Park, Sun-Dong ;
The Korea Journal of Herbology, volume 21, issue 2, 2006, Pages 37~46
Objectives : The purpose of this study was to investigate the effect of Dangguibohyultang (DB) and its combination (DB-I; Astragali membraneus BUNGE : Angelica gigas NAKAI=5:1, DB-II; Astragali membraneus BUNGE:Angelica gigas NAKAI=1:1, DB-III; Astragali membraneus BUNGE:Angelica gigas NAKAI=1:5,) on apoptosis in human colorectal adenocarcinoma HCT116 cells. Methods : To study the cytotoxic effect of methanol extract of DB-I, DB-II and DB-III on HCT116 cells, the cell viability was determined by XTT reduction method and ttypan blue exclusion assay. To confirm the induction of apoptosis, the cleavage of poly ADP-ribose polymerase (PARP), a substrate for caspase-3 and a typical sign of apoptosis, and the activation of procaspase-3, -8 and -9 were examined by western blot analysis. Furthermore, DB-induced apoptosis was confirmed by DNA fragmentation. The release of cytochrome C from mitochondria to cytosol, the level of Bcl-2 and Bax, and the expressions of Raf/MEK/ERK were examined by western blot analysis. Results : DB-I and DB-II reduced proliferation of HCT116 cells in a dose-dependent manner. DB-I and DB-II decreased procaspase-3, -8, -9 levels in a dose-dependent manner and induced the clevage of PARP. DB-I and DB-II also triggered the mitochondrial apoptotic signaling by increasing the release of cytochrome C from mitochondria to cytosol, decreasing of anti-apoptotic Bcl-2, and increasing of pro-apoptotic Bax. DB-I and DB-II decreased the activation of Ras/Raf/MEK/ERK cascade in a dose-dependent manner. Conclusion : These results suggest that DB-I and DB-II induce apoptosis via mitochondrial pathway in HCT116 cells. Furthermore, Raf/MEK/ERK cascade is involved in DB-induced apoptosis. These results suggest that DB is potentially useful as a chemotherapeutic agent in human liver cancer.
Study on the Development of Cosmetic Emulsion Cream for Patients with Atopic Dermatitis using Scutellaria Baicalensis
Park, Chan-Ik ;
The Korea Journal of Herbology, volume 21, issue 2, 2006, Pages 47~53
Objectives : This study was conducted to determine if Scutellaria baicalensis can be used in cosmetic emulsion cream for patients with atopic dermatitis. Methods : Scutellaria baicalensis extract was obtained with the use of butylene glycol through the pressurized solvent extraction(PSE). The antioxidative activity was assessed through SOD-like activity measurement and skin irritating potential was tested using human patch test. Antimicrobial activity was measured by the clear zone formed against Staphylococcus aureus and Escherichia coli and the rheological effects on the emulsion creams were examined using oscillation test. Results : The SOD-like activity increased dose-dependently and was about 90% at 1,000ppm of Scutellaria baicalesis extract. And Scutellaria baicalensis extract did not show any potential to be irritating to the human skin, but it could not be used as an antimicrobial agent for its poor antimicrobial activity against Staphylococcus aureus. The complex modulus decreased by 1,000 pascals and the loss angle also decreased by 20% with the addition of Scutellaria baicalensis extract into the cosmetic emulsion creams, that is, the extract can confer more elastic property on the vehicle. Conclusion : From those results, Scutellaria baicalensis extract can be effectively used as an antioxidant and reinforces the elastic skincare film formed by the application of cream for patients with atopic dermatitis.
Biological Activities of Extracts of Fermented Camellia japonica Leaf and Flower
Kim, Hyung-Woo ; Lee, Geun-Jin ; Cho, Su-In ;
The Korea Journal of Herbology, volume 21, issue 2, 2006, Pages 55~62
Objectives : This study was carried out to investigate the biological activities of methanol extracts of fermented Camellia japonica leaf and flower. Methods : Methanol extracts of fermented Camellia japonica leaf and flower were prepared and a dose of 100 and 400mg/kg/day was administered orally into mice. And after appropriate weeks, changes of serum enzyme activities were investigated to confirm its effects on serum glucose, cholesterol and short term administration safety. Results : Fermented flower extract showed significant decrease of serum level of cholesterol. And showed no toxicity on kidney and liver within the dose of 400mg/kg/day. Conclusion : Thus above result showed no toxicity on kidney and liver in male and female mice.
Liriopis Tuber improves stress-induced memorial impairments in rats
Kang, Young-Gun ; Lee, Tae-Hee ;
The Korea Journal of Herbology, volume 21, issue 2, 2006, Pages 63~75
Objectives : We investigated the effects of Liriopis Tuber on the nervous protection. Methods : We measured the reaction behavior with EPM(Elevated Plus Maxe) and MWM(Morris Water Maze) after applying restraint stress to rats. Also, the degrees of AchE generation were measured with immunohistochemical method in the regions of hippocampus, and the degree of TH generation in the regions of VTA and LC, respectively. Results : 1. As the results of measuring how long EPM which reflects anxiety reaction stayed in the open arm, there was the trend which can suppress anxiety reaction in the MMD+stress group(Liriopis Tuber+stress) but no statistical significance. The counting results how many EPM passed between opened and closed arm showed suppression trend against a physical activity in the saline+stress group but there was no statistical significance. 2. According to the result of MWM, the saline+stress group showed the learning retardation which means increased time arriving at goal compared to ti1e normal group at the second and third day of measurement. On the contrary, a learning retardation was significantly decreased in the MMD+stress group. Among the probe trial test a memory loss occurred in the saline+stress group, meanwhile memory ability was notably increased in the saline-stress group. 3. The degree of TH generation was investigated at the VTA and LC respectively after test animals treated with drug. In the saline+stress group, TH-immunoreactive cells were significantly increased, and these stress-induced TH increases were suppressed in the saline+stress group at the VTA region. However, the saline+stress group did not show any significant difference. 4. the degree of AchE generation was investigated at the CA3 of hippocampus. The saline+stress group showed that AchE-immunoreactive cells were significantly decreased. Those stress-induced reductions of AchE cells were meaningfully recovered in the saline+stress group. Although the cells showed recovery trend in the region of CA1 of hippocampus, statistical significance was not observed. Conclusion : The results of our study indicate that Liriopis Tuber can improve spatial memory ability of rats applied a restraint stress. This improvement of the spatial memory ability is considered to have an efficacy of nervous protection that Liriopis Tuber suppresses the generation of AchE in the hippocampus region and enhances the generation of TH in the VTA.
Effects of Coptidis Rhizoma and Aconiti Lateralis Preparata Radix on the Change of Plasma Corticosterone Level and Rectal Temperature Induced by LPS
Cho, Eun-Ho ; Lee, Tae-Hee ;
The Korea Journal of Herbology, volume 21, issue 2, 2006, Pages 77~85
Objectives : We investigated the effects of Coptidis Rhizoma and Aconiti Lateralis Preparata Radix on the LPS(Lipopolysaccharide) ICV(intracerebroventricular) injection. Methods : We measured plasma corticosterone level and rectal temperature in mice induced by I.C.V. injection of LPS (100ng/mouse). Results : The results were as follows.. 1. The plasma corticosterone levels in CR-1(0.5g/kg), CR-2(1.0g/kg), CR-3(3.0g/kg) were not decreased significant comparing with the control group.(P<0.05) 2. The plasma corticosterone level in AR-1(0.5g/kg) was decreased significant comparing with the control group.(P<0.05), but AR-2(1.0g/Kg) and AR-3(3.0g/kg) were not decreased significant comparing with the control group.(P<0.05). 3. The rectal temperature in CR-1(0.5g/kg), CR-2(1.0g/kg), CR-3(3.0g/kg) was decreased significant comparing with the control group.(P<0.05) 4. The rectal temperature in AR-1(0.5g/kg), AR-2(1.0g/kg), AR-3(3.0g/kg) was not decreased significant comparing with the control group.(P<0.05) Conclusion : These data revealed that Rhizoma Coptidis might have no significant effect on inflammation stress and Aconiti Lateralis Preparata Radix(0.5g/kg/mouse) might have significant effect on inflammation stress.
Cosmeceutical activities and Anti-inflammatory effects of Shell from Persicae semen
Joe, Woo-A ; Jang, Min-Jung ; Cheon, Soon-Ju ; Sung, Ji-Yeun ; Choi, Eun-Young ; Kang, Bo-Yeon ; Jung, Su-Hyun ; Jeung, Yeun-Suck ; Kim, Young-Sun ; An, Bong-Jeun ; Lee, Chang-Eon ; Lee, Jin-Tae ;
The Korea Journal of Herbology, volume 21, issue 2, 2006, Pages 87~93
Objectives : In this paper, we tested the applicability of shell from Persicae semen in cosmetics through cosmeceutical activities including anti-oxidant, tyrosinase inhibition and anti-inflammatory effects. Methods : Persicae semen, which had been extracted, concentrated, and freeze drying with water and ethanol, have been used for the experiment. The effects on electronic donating ability, SOD-like activity, xanthine oxidase inhibition, whitening effect have been investigated in the cosmeceutical activity measurement of function experiment. Results : In the electron donating ability test, 1,000ppm of EPS (ethanol extract of shell from Persicae semen) showed an effect of 87%. SOD-like activities showed 93% at the 10,000ppm of WPS (water extract of shell from Persicae semen). In the xanthine oxidase inhibition test, 1,000ppm of BHA showed an effect of 27%, while EPS showed an effect of 62%. We were able to get an effect of 95% from EPS at 10,000 ppm in the tyrosinase inhibition test. In the anti-inflammatory test, the EPS inhibited the generation of nitric oxide. In the case of the EPS, there were no signs of cytotoxicity against raw 264.7 and anti-inflammatory effects could be identified when the manifestation of iNOS was decreased. Conclusion : Therefore, the EPS has potential as an effective raw materials for cosmetic.
Studies on the Cosmeceutical Activities of Perilla frutescens var. acuta
Choi, Eun-Young ; An, Bong-Jeun ; Lee, Chang-Eon ; Jeung, Su-Hyun ; Jang, Min-Jung ; Cheon, Soon-Ju ; Sung, Ji-Yeun ; Kang, Bo-Yun ; Joe, Woo-A ; Jeung, Yeun-Suck ; Choi, Hyang-Ja ; Baek, Uk-Jin ; Lee, Jin-Tae ;
The Korea Journal of Herbology, volume 21, issue 2, 2006, Pages 95~101
Objectives : This study aimed to establish cosmeceutical activities of Perilla frutescens var. acuta. Methods : Folium Perillae, which had been extracted, concentrated, and freeze drying with water and ethanol, have been used for the experiment. The effects on electronic donating ability, SOD-like activity, xanthine oxidase inhibition, whitening effect have been investigated in the cosmeceutical activity measurement of function experiment. Results : Both water and ethanol extracts of Perilla frutescens var. acuta showed relatively high electron donating ability of more than 70% at over 500ppm. Also, SOD like activity increased in a dose dependent matter of more than 80% at 5,000ppm, while tyrosinase showed insufficient inhibitory rate. Xanthine oxidase showed a meaningful inhibitory effect of 72.4% in water extract and 55.3% at 1,000ppm in ethanol extract. The addition of
showed relatively high oxidizing inhibitory effect of 57% and 41%, respectively, at 500ppm in water extract and, when
ion was added in ethanol extract, the effect of 64% at 1,000ppm was achieved. Also, higher oxidizing inhibitory effect was shown against
in both water and ethanol extracts. For human cancer cells, relatively high growth inhibition ability was shown against melanoma G361 in both water and ethanol extracts. Conclusion : From the above results, it was confirmed that Perilla frutescens var. acuta could be used in functional cosmetics.
Study of the Cosmeceutical Activities of Cuscuta japonica Choisy
Joe, Woo-A ; Cheon, Soon-Ju ; Jang, Min-Jeong ; Sung, Ji-Yeun ; Jung, Su-Hyun ; Kang, Bo-Yeon ; Choi, Eun-Young ; Jeong, Yeon-Sook ; Lee, Joon-Sook ; Choi, Ho-Jung ; Baek, Wook-Jin ; An, Bong-Jeun ; Lee, Chang-Eon ; Lee, Jin-Tae ;
The Korea Journal of Herbology, volume 21, issue 2, 2006, Pages 103~108
Objectives : The cosmeceutical activity of Cuacuta japonica Choisy were investigated for cosmetics industrial application. Methods : Cosmeceutical activities include antioxidant, tyrosinase inhibition effects and nitrite-scavenging ability were examined through the Cuacuta japonica Choisy Results : The result were obtained as follows : The electron donating ability of water and ethanol extract was 69.5%, 78.3% at 1,000ppm. In SOD-like activity, water extract showed low effect, but ethanol extract scored 63.4% at 1,000ppm. In inhibition activity of xanthine oxidase, water extract showed low effect, which is 27.2% at 500ppm, in contrast to ethanol extract high effect: 99.5% at 500ppm, in nitrite-scavenging ability test, 500ppm of C. japonica Choisy water extract showed the effect of 51.3%, while 500ppm of C. japonica Choisy ethanol extract showed the effect of 72.7%. From the test of inhibitory effect of tyrosinase, both water and ethanol extract showed insufficient inhibitory rate. Conclusion : According to all the test results that have been given, it is possible that the extract of C. japonica Choisy can be used as a new natural material of the cosmeceutical industry.
The Approach of Properties-Flavours Theory and the Study of Morphological Standard in CORNI FRUCTUS
Choi, Mun-Il ; Lee, Hwa-Jung ; Yun, Ju-Bong ; Kim, Ja-Young ; Kang, Kyoung-Sik ; Shin, Chol-Gyun ; Ju, Young-Sung ;
The Korea Journal of Herbology, volume 21, issue 2, 2006, Pages 109~119
Objectives : This study was performed to approach of properties-flavours theory and the study of morphological standard in CORNI FRUCTUS. Methods : The properties-flavours theory were attempted with bibliographic method and various climate-information. The external-internal morphological standards were determined by using stereoscope and butanol series. Results and conclusion : 1. The significant analysis for the corni fructus as the properties-flavours theory, is a follow-up survey of the effect written in the official oriental medicine book. 2. The other analytic methods according to the gathering time, processing or storing system or especially nature or growth environment(altitude, temperature,. the mean moisture, and the agronomical survey) by way of the explanation of properties-flavours theory appears so many insufficiency at the many cases. 3. The external and internal characteristics according to the shape of original plant or herbal states, entirely correspond to all the literature on this subject. 4. According to the place of production, there are some different points. (l) In the external shapes, it is reasonable that if we make the basic standard of classification by the sharpness of color, the existence of gloss and the size of products. In the case of the Korean products, it shows bright and glossy color totally, but in the case of North Korea products, it shows black and lackluster. And in the case of the foreign products, it has softer body than Korea one and shows white color on the surface. (2) In the internal shapes, the Korea products has a thicker cuticle layer than the foreign one, and has fewer ca-oxalate in the parenchyma cell.
The Inhibitory Effects of Poncirin against Nickel Induced Cytotoxicity
Yang, Seung-Jin ; Kwak, Dong-Keun ; Han, Du-Suk ;
The Korea Journal of Herbology, volume 21, issue 2, 2006, Pages 121~127
Objectives : Nickel is a major metal used in the nickel-chromium alloys of most orthodontic appliances, partial denture and implants. This study was carried out for the examination of the cytotoxicity on nickel sulfide in cultured NIH3T3 fibroblasts, and poncirin effect on nickel-induced cytotoxicity. Methods : Cell viability for the MTT assay and cell adhesion activity for the XTT assay. Results : The
of nickel sulfide by the MTT assay was
. Poncirin was significantly increased the cell viability and cell adhesion activity. Conclusion : Nickel was highly toxic and poncirin has the inhibitory effects against nickel induced cytotoxicity.
Identification of Cervus elaphus Species by Sequencing Analysis and BLAST Search
Seo, Jung-Chul ; Kim, Min-Jung ; Lee, Chan ; Leem, Kang-Hyun ;
The Korea Journal of Herbology, volume 21, issue 2, 2006, Pages 129~133
Objectives : Cervus elaphus species are some of the most medicinally important genera in the Oriental medicine. This study was performed to determine if Cenvus elaphus species could be identified by sequencing analysis and to verify Basic Local Alignment Search Tool (BLAST) search, which was used to assess genetic identification. Methods : The DNAs of Cervus elaphus species were extracted, amplified by PCR, and sequenced. The DNAs of Cervus species were identified by BLAST search in website. Results : By BLAST search one of Cervus elaphus species was identified as Cervus elaphussibericus but the other was identified as Cervus elaphus nelsoni. This work showed that identification can efficiently be performed by BLAST search. Conclusion : These results suggest that sequencing following BLAST search might be able to provide the identification of Cervus elaphus species.
Inhibition of gene expression and production of iNOS and
in LPS-stimulated microglia by On-Bi-Tang
Son, Hye-Young ; Kim, Sang-Woon ; Jung, Sun-Ju ; Jung, Hyo-Won ; Yoon, Cheol-Ho ; Park, Yong-Ki ;
The Korea Journal of Herbology, volume 21, issue 2, 2006, Pages 135~142
Objectives : On-Bi-Tang(OB) has been prescribed Chinese traditional medicine for the treatment of inflammatory disease such as chronic renal failure. In this study, we investigated the anti-inflammatory effect of OB extract in the BV2 murine microglial cells. Methods : After the water extract of OB was treated in BV2 cells, murine microglial line, the cell viability was measured by MTT assay. The production of nitric oxide (NO) and
was determined based on Griess reagent and enzyme linked immunosorbant assay (ELISA). mRNA expression of inducible nitric oxide synthase (iNOS) and
was analyzed by RT-PCR. Results : OB extract significantly inhibited the LPS-induced production of NO and TNF-a in BV2 cells. OB extract also suppressed the mRNA expression of iNOS and
in BV2 cells activated with LPS. Conclusion : These data suggests that OB extract may have the anti-inflammatory effect through the modulation of NO production and inflammatory cytokine such as
The Effect of Woohwang with Pear Phenolic compound on Blood Pressure, Plasma Renin, ANP in Hypertensive Rat Induced by 2K1C
Youn, Dae-Hwan ;
The Korea Journal of Herbology, volume 21, issue 2, 2006, Pages 143~150
Oriental pear was used as treatment of asthma, control of blood pressure tonic medicine diabetes in oriental medicine, Pear Pectin was effective on control of blood pressure in previous report. In this study, it was investigated that Woohwang with pear extractions effects on cardiovascular system as blood pressure and renin and Atrial natriuretic peptide(ANP) in plasma. The 2K1C hypertension model was prepared by constricting the left renal artery with a sliver dip. Animals were then divided into three groups, control, Woowhang:Pear Phenolic compound(1:1), Woowhang:Pear Phenolic compound(2:1),Woowhang:Pear Phenolic compound(1:2) were supplied with them. Direct-blood pressure was measured at femoral vein, Indirect-blood pressure was measured at rodent tail. The results are as follows. The blood pressure was more significantly decreased at 1:2(woohwang:pear phenolic compound)group than other groups. On 6,9,12,15days, the blood pressure was significantly decreased in 1:2(woohwang:pear phenolic compound)group. The plasma ANP was significantly increased in 1:2(woohwang:pear phenolic compound)group. It tenders to decrease in 1:2(woohwang:pear phenolic compound)group on plasma renin. Based on the above results it is assumed that oral administration of Woohwang with Pear Phenolic compound(1:2) can help the treatment of hypertension.
Neuroprotective Effect of
on Transient Focal Cerebral Ischemia in Rats
Bu, Young-Min ; Oh, Se-Nam ; Hwang, Man-Ki ; Chung, Jin-Hee ; Lee, Dae-Hee ; Park, Young-Mee ; Kim, Mi-Yon ; Kim, Zhen-Hwa ; Kim, Ho-Cheol ;
The Korea Journal of Herbology, volume 21, issue 2, 2006, Pages 151~158
Objectives : The purpose of the present study is to observe the neuroprotective effect of the
on transient focal cerebral ischemia in rats. Methods :
was made by mixing the herbs, mainly the root of Panax ginseng, the root of Rehmannia glutinosa and Poria cocos, the stem bark of Acanthopanax senticosus, the root of Scutellaria baicalensis and Mel, and heating for 96 hours. Transient Focal cerebral ischemia (2 h of ischemia, 22 h of reperfusion) was induced by intraluminal suture method with SD rats. Sensory motor function was tested by rotarod test, prehensile traction test, beam balance test and foot fault test at 24 h after ischemia. The brain slices were stained by 2% 2, 3, 5-triphenyltetrazolium chloride and the infarct volume was measured by graphic analyzer at 24 h after ischemia. Results :
treated group did not show significant differences compared with vehicle treated group in body temperature. Oral administration of
reduced brain infarct volume by 29.7% compared with vehicle treated group.
also showed protective effects on sensory motor functional deficits. Conclusion :
treatment reduced brain damage and improved functional deficits induced by MCAo. It showed neuroprotective effects even when treatment was relayed 2 h after injury. Further research is required to evaluating long term functional recovery am accurate therapeutic range and mechanisms.
A 4-weeks Oral Toxicity of Dangguibohyel-tang in Sprague-Dawley Rats
Kang, Soon-Ah ; Chang, Mun-Seog ; Oh, Myung-Sook ; Park, Wan-Su ; Kim, Won-Nam ; Yang, Woog-Mo ; Lee, Byong-Hee ; Park, Seong-Kyu ;
The Korea Journal of Herbology, volume 21, issue 2, 2006, Pages 159~163
Objectives : The subacute toxicity was investigated in Sprague-Dawley rats orally treated with Dangguibohyel-tang (DBT). DBT has been used for anemia, in Korean medicine. Methods : Sprague-Dawley rats were administered orally with DBT at the dosages of 1,000 mg/kg for 14 days. We daily examined number of deaths, clinical signs, body weights and gross findings during the 14 days. Results : There were no clinical signs and pathological changes compared with control group. Body weights were not significantly changed between control and treatment groups. In hematological and biochemical serum parameters, all mean values appear to be within the normal range. Conclusion : These results suggest that DBT dose not induce any significant subacute oral toxicities in Sprague-Dawley rats.
Inhibitory effects of Coptidis Rhizoma on the LPS-induced production of nitric oxide and
in mouse macrophage cells
Jung, Hyo-Won ; Park, Yong-Ki ;
The Korea Journal of Herbology, volume 21, issue 2, 2006, Pages 165~173
Objectives : Coptidis Rhizoma has been known traditional medicine with antimicrobial activities. We investigated inhibitory effects of Coptidis Rhizoma extract on lipopolysaccharide(LPS)-induced nitric oxide production from mouse macrophages. Methods : After Coptidis Rhizoma extract was pretreated in BV2, mouse brain macrophages and RAW264.7 mouse macrophages, cells were activated with LPS. To investigate cytotoxicity Coptidis Rhizoma extract, cell viability was measured by MTT assay. The production of nitric oxide(NO) and inducible nitric oxide synthase(iNOS) was determined in each culture supernatant and mRNA by Griess reaction and RT-PCR. The production of
from cells was measured by ELISA. Results : Coptidis Rhizoma extract significantly inhibited LPS-induced NO production in BV2 and RAW264.7 cells. Coptidis Rhizoma extract also greatly suppressed mRNA expression of iNOS in BV2 and RAW264.7 cells activated by LPS. Conclusion : These data suggests that Coptidis Rhizoma extract may have an anti-inflammatory effect through the inhibition of NO production.
A Study on the Biophoton Emission of Cervi Pantotrichum Cornu
Park, Wan-Su ; Lee, Chang-Hoon ; Soh, Kwang-Sup ; Kim, Ho-Cheol ; Choi, Ho-Young ; Park, Seong-Kyu ;
The Korea Journal of Herbology, volume 21, issue 2, 2006, Pages 175~180
Objectives : The difference of delayed luminescence-biophoton emission was investigated in Cervi Pantotrichum Cornu selected randomly. Cervi Pantotrichum Cornu was used as a tonic in Korean medicine. Methods : Randomly selected samples of Cervi Pantotrichum Cornu were radiated with 150 W metal halide lamp for 1 minute. After radiation, biophoton emissions of each sample were detected by electron multiplication(EM)-charge coupled device camera. The detected biophoton image was calculated with unit of counts per pixel. Results : The average biophoton emissions of delayed luminescence with EM ratio of
were distinguished significantly. The maximum biophoton emissions of delayed luminescence with EM ratio of
were distinguished significantly. Conclusion : These results suggest that biophoton imaging of Cervi Pantotrichum Cornu could become the meaningful method for the study of differentiation and classification of Cervi Pantotrichum Cornu.
A Study on a Morphological Identification of Ostericum Root
Kim, Sung-Hyum ; Kil, Ki-Jung ; Lee, Young-Jong ;
The Korea Journal of Herbology, volume 21, issue 2, 2006, Pages 181~188
Objectives : Southern, northern, and wild types of Osterici Radix, and Notopterigii Rhizoma et Radix imported from China are circulated in Korea. Morphological identification was attempted by comparing external and internal forms of these materials. Methods : The slice of the tested material made by paraffin section technique was colored with Safranine Malachite Green contrast methods, and then observed and photographed by olymphus-BHT. Results : 1. Southern type of Osterici Radix has short rhizome and many small and long roots, while northern type of it is mostly composed of rhizomes with little small reds and sometimes looks like bamboo because of the extention of the parts between joints. 2. Parenchymal cell of southern type of Osterici Radix is filled with starch grain, while that of nothern type includes less starch grain with lamination crossed on its cell wall. 3. Surface of root and rhizome of wild Osterici Radix has some marks of fibrous root raised in the form of nodule, but cultivated Osterici Radix does not have this kind of marks. 4. Notopterigii Rhizoma et Radix is mostly composed of rhizomes, and its form is similar to that of a silkworm or joints of bamboo. Parenchymal cell is filled with starch grains, and lamination is crossed on its cell wall but not salient. Conclusion : Southern, northern, and wild types of Osterici Radix, and Notopterigii Rhizoma et Radix circulated in Korea can be easily identified because of their differences in their external and internal forms.
Anti-inflammatory effect of extract of Asarum sieboldii in LPS-stimulated Murine peritoneal macrophage
Jung, Won-Seok ; Yoo, Hyeon-Mi ; Seo, Sang-Wan ; Cho, Joon-Ki ; Son, Ji-Woo ; Park, Min-Cheol ; Choi, Chang-Min ; Yeom, Seung-Ryong ; Hwang, Sang-Wook ; Kim, Yong-Woo ; Song, Dal-Soo ; Chae, Young-Seok ; Kim, Yeong-Mok ; Park, Sung-Joo ; Shin, Min-Kyo ; Song, Ho-Joon ;
The Korea Journal of Herbology, volume 21, issue 2, 2006, Pages 189~195
Objectives : The purpose of this study was to investigate the anti-inflammatory effects of extract Asarum sieboldii(AS) on the peritoneal macrophage. Methods : To evaluate of anti-inflammatory of AS, we examined cytokines production in lipopolysacchride (LPS)-induced macrophages. Furthermore, we checked molecular mechanism using western blot. Results : 1. Extract from AS reduced LPS-induced Nitric oxide (NO), tumor necrosis factor-a (TNF-a), interleukin (IL)-6 and IL-12 production in peritoneal macrophages 2. Extract from AS itself does not have any cytotoxic effect. AS inhibited the activation of mitogen-activated protein kinases (MAPKs) such as p38, extracelluar signal-regulated kinase B a (IkBa) in the LPS-stimulated peritoneal macrophages Conclusion : AS down-regulated LPS-induced NO and cytokines production, which could provide a clinical basis for anti-inflammatory properties of AS