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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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The Korea Journal of Herbology
Journal Basic Information
Journal DOI :
The Korea Association of Herbology
Editor in Chief :
Volume & Issues
Volume 22, Issue 4 - Dec 2007
Volume 22, Issue 3 - Sep 2007
Volume 22, Issue 2 - Jun 2007
Volume 22, Issue 1 - Mar 2007
Selecting the target year
A study of "administration time in herbal medicine"
Park, Pil-Sang ; Kang, Ok-Hwa ; Lee, Go-Hoon ; Park, Shin-Young ; Seong, Man-Jun ; Kim, Yeong-Mok ; Song, Ho-Joon ; Kwon, Dong-Yeul ;
The Korea Journal of Herbology, volume 22, issue 4, 2007, Pages 1~8
Objectives : There are many causes that may affect efficacy of a drug but the time of administration for the Herbal Medicine is among the most important factors. Traditionally, administration of medicine was prohibited during the meal time along with food and the time of administration has been highly regarded in medical practitioners. The rules of administration time are stated in a book titled, "Materia Medica" but it lacked the details and index. Thus, the systemized administration rules for Herbal Medicine were prepared. Methods : The rules were drawn from both ancient teachings and the experiences from modern clinics. Ancient teachings about the administration are focused on treatment of a disease by utilizing biorhythm of the body in accordance of changes in Yin and Yang and Day and Night. Results : It means the medicine should be prepared and administered at the appropriate time of the day and this type of administration method is thought to be superior to the western method such as "once a day" or "twice a day in the morning and evening" that does not take consideration of cold, hot, warm and cool properties of the medicine. If the prescription is assigning appropriate medicine, administration time is an important method of maximizing drug efficacy. Conclusion : With the ancient teaching in mind, it was concluded that, the drug administration time must be determined with regard to properties of the drug and the condition of the patient.
Gene Expression of Hyperthyroid Rats treated by Scrophularia buergeriana Miquel
Cho, Chung-Sik ; Kim, Dae-Bok ; Kim, Cheol-Jung ;
The Korea Journal of Herbology, volume 22, issue 4, 2007, Pages 9~19
Objective : The study was done to investigate Scrophularia buergeriana Miquel's effects in the genomic level, by measuring gene expression in hyperthyroid induced rats using cDNA micro array. Methods : Spargue-Dawley rats were separated into three groups(each with 10 rats). Except normal group, the other two groups were treated with Sodium levothyroxine
/kg/days for 5 days by oral administration. After 2 hours of the last intake of Sodium levothyroxine on two experimental group, one group was treated Scrophularia buergeriana Miquel extract 1.0g/kg/days for 3 days. The other groups(normal group, administration group) was treated normal saline 1.0g/kg/days for 3 days. Gene expression of hyperthyroid rats were measured after medication of solid extract of Scrophularia buergeriana Miquel with cDNA microarray. Results : In thyroid tissue, the numbers of the genes showed over twice increase and decrease in the control group compared to the normal group were 296, in the Scrophularia buergeriana Miquel administration group compared to the normal group were 859 and in the hypothalamus, the numbers of the genes showed over twice increase and decrease in the control group compared to the normal group were 416, in the Scrophularia buergeriana Miquel administration group compared to the normal group were 391. Conclusion : According to the above results, it is suggested that Scrophulari buergeriana Miquel suppress hyperthyroidism effectively and regulate the gene expression in the thyroid and brain.
Purification and characterization of Protease from Kyenegum
Kim, Do-Wan ; Jo, Hye-Sim ; Jeong, Yong-Jin ; Kim, Kwang-Soo ;
The Korea Journal of Herbology, volume 22, issue 4, 2007, Pages 21~28
Objectives : Kyenegum has been popularly used long as the digestive. The purpose of this study was to investigate the purification and characteristics of protease obtained from Kyenegum crude enzyme. Methods : Kyenegum protease was purified by precipitation with ammonium sulfate followed by SP-Spharose ion exchange chromatography. The molecular weight of Kyenegum protease was estimated by SDS-PAGE electrophoresis. Results : Kyenegum protease was 3,087 units/mg protein specific activity, 14.5 purification fold and 9.8 % recovery. The molecular weight of protease was estimated to be 18 kDa. The isoelectric point was pI 8.97 and values of Km and Vmax of its were 48 mg/mL and 2 units/min, respectively. Conclusion : The result suggests that the protease obtained from Kyenegum has excellent stability of temperature, acid and collagen substrate specificity.
A Enzymatical Characteristics Study of Kyenegum
Kim, Do-Wan ;
The Korea Journal of Herbology, volume 22, issue 4, 2007, Pages 29~34
Objective : Kyenegum(Galli Stomachichum Corium) has been popularly used long as the digestive. The purpose of this study was to investigate the enzymatic characteristic of Kyenegum crude enzyme. Methods : To evaluate of the enzymatic characteristic of Kyenegum, we examined the activity of Kyenegum crude enzyme from optimum solvent, optimum temperature and pH of crude Kyenegum extract. Futhermore, we examined the effects of NaCI and acidity of crude Kyenegum extract. Results : The Kyenegum was composed with crude protein about 20%, crude lipid 2%. The optimum Kyenegum dry condition, optimum extract solvent, optimum temperature and optimum pH were
, commercial apple vinegar,
and 2.0. Conclusion : The result suggests that the Kyenegum crude enzyme extract very strong enzyme in temperature, NaCl and acidity, respectively.
The Effects of Daeyoungjeon(DYJ) on the Hypothyroidism in Rats
Cho, Chung-Sik ; Kim, Du-Hui ; Choi, Jeong-Sik ; Kim, Cheol-Jung ;
The Korea Journal of Herbology, volume 22, issue 4, 2007, Pages 35~43
Objective : The aim was to study experimentally the effects of Daeyoungjeon(DYJ) on the hypothyroidism of rats induced by thiouracil. Methods : Spargue-Dawley rats were separated into four groups. Except normal group, the other three groups were treated with thiouracil 0.50g/kg/days for 30 days by oral administration. Among the three groups, one group was treated DYJ extract 0.75g/kg/days for 20 days separately. Another group was treated DYJ extract 1.50g/kg/days for 20 days separately. T3-uptake, T3, T4, TSH, hematological values, enzyme activity, total cholesterol, triglyceride and Na+ content change in the Serum of rats were measured after medication of solid extract of DYJ. Results : As a result of this study, in the serum T3-Uptake and T4 level, hematological values and sodium were increased in DYJ group compared to control group. The serum TSH, total cholesterol, triglyceride, enzyme activity and body weight were decreased in DYJ group compared to control group. Conclusion : According to the above results, it is suggested that DYJ is effective to cure hypothyroidism.
A study on the Gypsum and Natrii sulfas dose of 1 day and 1 time in Shanhanlun and Gumgyeyoryak
Jeon, Sae-Hun ; Kim, In-Rak ;
The Korea Journal of Herbology, volume 22, issue 4, 2007, Pages 45~50
Objectives : The purpose of this study is to determine dose of Gypsum and Natrii sulfas in Shanhanlun and Geumgyeyoryak Methods : We measured the egg size Gypsum, and Natrii sulfas 1 Seong(升), and Results : 1. The weight of GYPSUM, egg is about
, it will end up with 104g, when applying egg gravity of 1.055 and Gypsumls weight of 2.3. 2. One Ryang(兩) is equal to 6.5g in Sanghanlun and Gumgyeyoryak, and therefore the weight of ones gypsum is equal to 1 Keun(斤). 3. Compare to a dose of Gypsum is equal to one egg in Mokbanggitang. 4. Maximum dosage of Gypsum is equal to half an egg, 8Ryang(兩) of Sanghanlun, which is 52g. 5. Daily dosage of One Keun(斤) of Natrii sulfas is 8 Ryang(兩), of sanghanlun, which is 52g, Maximum dosage of Sanghanlun is 4 Ryang(兩), which is 26g. Conclusion : As stated above we may acknowledge that a dosage of Gypsum and Narii sulfas in either Sanghanlun and Keumgyeyoryak is applicable range in practical clinic.
Enhancing effect of Multiherb extracts HT008-1 on Memory and Cognitive Function
Seo, Joo-Hee ; Woo, So-Young ; Kim, Yun-Tai ; Kim, Mi-Yeon ; Jin, Zhen-Hua ; Park, Young-Mi ; Bu, Young-Min ; Kim, Ho-Cheol ;
The Korea Journal of Herbology, volume 22, issue 4, 2007, Pages 51~58
Objectives : Investigation of the memory and cognitive enhancing effect of HT008-1 in scopolamine induced amnesia mice. Methods : At 60 min before acquisition trials, HT008-1 (30, 100, 300 mg/kg p.o.) was administered, and 30 min later, mice were injected with scopolamin (1.0 mg/kg, i.p.). In the passive avoidance test, acquisition trials were carried out 30 min after a single scopolamine treatment. Retention trials were carried out 24h after acquisition trials. Y-maze test was carried out 30 min after a single scopolamine treatment. Spontaneous alternation behavior during an 8-min session was recorded. Inhibitory effects of HT008-1 (0.01, 0.1, 1.0 mg/ml) on AChE activity was measured. Result : HT008-1 ameliorated scopolamine-induced learning impairments and spatial cognitive function in passive avoidance and Y-maze test, respectively. Moreover HT008-1 showed a significant inhibitory effect on AChE activity. Discussion: This study presented that eMultiherb mixture HT008-1 enhanced learning memory and spatial cognitive function in scopolamine-induced amnesia mice. These results suggest that the effect of HT008-1 may be dependent on the inhibition of AChE activity.
Subacute Toxicity Study on Palmultang(Bawu-Tang) in SD Rats
Ma, Jin-Yeul ; Yu, Young-Beob ; Ha, Hye-Kyung ; Huang, Dae-Sun ; Shin, Hyun-Kyoo ;
The Korea Journal of Herbology, volume 22, issue 4, 2007, Pages 59~64
Objectives : Palmultang(Bawu-tang) has been traditionally prescribed as a restorative, The present study was undertaken to determine the possible toxic effects of Palmultang on SD rats. Methods : In this study, we investigated the subacute toxicity of water-extracted Palmultang(Bawu-tang) on SD rats. Twenty rats were orally adiministered Palmultang for 28 days at a dose of 0 mg/kg(control group) or 1500 mg/kg(treated group), respectively. Results : All of subjects was survived. No significant difference in abnormal clinical signs, related to hematological values, serum biochemical values, water and feed intake, coagulation time, autopsy analysis, organ weight, tissue microscopically, funduscopy, urine intake and urinalysis, was detected. Conclusions : Compared with the control group, we could not find any subacute toxic alteration in treated group (1500 mg/kg) for 28 days. This result suggests that Palmultang, a herbal medicine prescription, is a safe prescription to body.
Anti-inflammatory effect of Salviae Miltiorrhizae Radix
Yun, Hyun-Jeong ; Heo, Sook-Kyoung ; Yun, Hyung-Joong ; Park, Won-Hwan ; Park, Sun-Dong ;
The Korea Journal of Herbology, volume 22, issue 4, 2007, Pages 65~73
Objective : Salvia miltiorrhiza Bunge (Labiatae) (SM), an eminent herbal plant, has been widely used in traditional Chinese medicine for the treatment of vascular diseases such as hypertension. The aim of this study was to determine whether SM inhibits production of nitrite, an index of NO, and proinflammatory cytokines in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages. And this study investigated whether or not SM could reduce tumor necrosis factor-
)-induced inflammatory response in human vascular aortic smooth muscle cells (HASMC) and umbilical vein endothelial cells (HUVEC). Methods : Cytotoxic activity of SM on RAW 264.7 cells was using 5-(3-caroboxymeth-oxy phenyJ)-2H-tetra-zolium inner salt (MTS) assay. We measured the NO production using Griess Reagent System. Production of Proliflammatory cytokines was measured by Enzyme-Linked Immunosorbent Assay (ELISA). Results : Our results indicated that SM significantly inhibited the LPS-induced NO production accompanied by an attenuation of tumor necrosis factor-
), IL-6 and monocyte chemoattractant protein (MCP)-1 formation in macrophages. SM decreased TNF-
-induced IL-8, IL-6 production, and intracellular adhesion molecule (ICAM)-1 and vascular cell adhesion molecule (VCAM)-1 expression. Conclusion : These results indicate that SM has potential as an anti-inflammatory agent.
New Method for The Prevention of Microbial Contamination in Deer Antler Manufacturing Process
Park, Seong-Kyu ; Yang, Woong-Mo ; Kim, Hong-Yeoul ; Chang, Mun-Seog ;
The Korea Journal of Herbology, volume 22, issue 4, 2007, Pages 75~80
Objectives : This study was conducted to control microorganisms of deer antler products with ethanol and heat process. Methods : The deer antler of Cervus elaphus was used for this study. The sliced deer antler of market condition were processed with 70% ethanol only (酒洗) and 70% ethanol with heat (酒炙). The microorganisms were isolated and incubated on Luria broth (LB) plates at
for 24 h. Results : The number of isolated microorganism colony were 201.1, 33.5 and 2.0 ea from each sliced deer antler of market condition, 70% ethanol only and 70% ethanol with heat process, respectively. Conclusions : These results suggested that 70% ethanol with heat processing is effective for reducing microbial contamination of deer antler products.
Antioxidant Effects of Rehmanniae Radix Preparat in GC-1 Cells
Park, Seong-Kyu ; Chang, Mun-Seog ; Yang, Woong-Mo ; Yu, Tae-Weon ; Kim, Do-Rim ; Park, Eun-Hwa ; Ko, Eun-Bit ; Choi, Moon-Jung ; Kim, Hyu-Young ; Oh, Ji-Hoon ; Shim, Kyung-Jun ; Yoon, Ji-Won ;
The Korea Journal of Herbology, volume 22, issue 4, 2007, Pages 81~86
Objectives : The purpose of this study is to examine the antioxidant activity in the germ cells of the extract of Rehmanniae Radix Preparat (RR). Methods : The extract was studied for diphenyl-picryl-hydrazyl (DPPH) radical scavenging activity, GC-1 cell viability by a modified MTT assay, the effects on H2O2-induced cytotoxicity by MTT assay and lipid peroixidation by malondialdehyde (MDA) formation, respectively. Results : The results showed that the extract scavenged DPPH radical in a dose-dependent manner by up to 43.1%. The extract at concentrations of 100
showed peak level of 136.5% in growth of GC-1 cell. The hydrogen peroxide-induced cytotoxicity was blocked by the extract at concentrations of 10, 50 and 100
. The extract (50 and 100
) also displayed a dose-dependent reduction of MDA formation on hydrogen peroxide-induced lipid peroxidation. Conclusions : In conclusion, the extract of Rehmanniae Radix Preparat has strong antioxidant activity.
Inhibitory Effects of Nelumbo nucifera on Tyrosinase Activity and Melanogenesis in Clone M-3 Melanocyte Cells
Park, Seong-Kyu ; Chang, Mun-Seog ; Kim, Hyang-Mi ; Yang, Woong-Mo ; Kim, Do-Rim ; Park, Eun-Hwa ; Ko, Eun-Bit ; Choi, Moon-Jung ; Kim, Hyu-Young ; Oh, Ji-Hoon ; Shim, Kyung-Jun ; Yoon, Ji-Won ;
The Korea Journal of Herbology, volume 22, issue 4, 2007, Pages 87~94
Objectives : We examined three parts of Nelumbo nucifera that might be useful for skin-whitening effect. Each parts of leaves, flowers and stamen were extracted with water or 70% ethanol. Methods : This study investigated inhibitory effects of each extracts on tyrosinase activity. Extracts were tested for cytotoxicity on clone M-3 melanocyte cells, melanin inhibitory activities were further assessed. Results : The water extract of Nelumbo nucifera stamen. ethanol and water extracts of flowers exhibited inhibitory effects on tyrosinase (IC50 values 726.16, 1063, and 1732.36 ug/mL, respectively). The water extract of Nelumbo nucifera stamen, ethanol extract of flowers, both ethanol and water extracts of leaves showed relatively lower cytotoxicity, with cell viability above 80% with a concentration of 20
. In addition, The water extract of Nelumbo nucifera stamen inhibited the melanin production in clone M-3 melanocyte cells. Conclusions : Among each parts of Nelumbo nucifera. the water extract of stamen was the strongest candidates for skin-whitening cosmetic application.
Study on Biophoton Emission from roots of Angelica gigas N., Angelica sinensis D., and Angelica acutiloba K
Park, Wan-Su ; Lee, Chang-Hoon ; Soh, Kwang-Sup ; Lee, Young-Jong ; Lee, Choong-Yeo ; Lee, Tae-Hee ; Kim, Youn-Sub ; Kim, Do-Hoon ;
The Korea Journal of Herbology, volume 22, issue 4, 2007, Pages 95~100
Objectives : The purpose of this study is to investigate the delayed luminescence-biophoton emission from roots of Angelica gigas N., Angelica sinensis D., and Angelica acutiloba K These three species of Genus Angelica are now used as 'Danggui' in Traditional Korean Medicine. Methods : Randomly selected samples from roots of Angelica gigas N., Angelica sinensis D., and Angelica acutiloba K were radiated with 150 W metal halide lamp for 1 minute. After radiation, biophoton emissions of each sample were detected by electron multiplication-charge coupled device camera. The detected biophoton image was calculated with unit of counts per pixel. Results : The average and maximum biophoton emissions of delayed luminescence with electron multiplication ratio of
were distinguished significantly between Angelica gigas N. and the other two species. Conclusions : These results suggest that biophoton imaging of roots of Angelica gigas N., Angelica sinensis D., and Angelica acutiloba K. could become the meaningful method for the study of differentiation between root of Angelica gigas N. and the other two species, Angelica sinensis D. and Angelica acutiloba K.
The anti-oxidant effects of Ligusticum chuanxiong, Cnidium officinale and their mixture with Angelica gigas
Park, Yong-Ki ;
The Korea Journal of Herbology, volume 22, issue 4, 2007, Pages 101~108
Objectives : To compare the anti-oxidant effects of Ligusticum chuanxiang and Cnidium officinale extracts and their mixture with Angelica gigas, we investigated the anti-oxidative activities using rat liver tissues. Methods : We investigated the anti-oxidative activities by analysis of lipid peroxidation, xanthine oxidase activity, aldehyde oxidase activity, hydroxyl radical scavenging activity, superoxide dismutase activity and catalase activity in rat liver tissues. Results : Both Ligusticum chuanxing and Cnidium offieinale inhibited the lipid peroxidation compared to the control, there is no significant differences between two groups. Cnidium officinale showed strong inhibitory effects on xanthine oxidase and aldehyde oxidase activities compared with that of Ligusticum chuanxing. Ligusticum chuanxing and Cnidium officinale were scavenged the hydroxy radicals, and increased SOD and catalase activities. These results suggested that Ligusticum chuanxing and Cnidium officinale were showed antioxidant activity, especially Cnidium officinale showed higher activity than Ligusticum chuanxing. Conclusions: These results suggest that Ligusticum chuanxing could be replaced by Cmdium afficinale in clinical trial.
Anti-inflammatory effect of chloroform fraction of Coptidis rhizoma on the production of inflammatory mediators from LPS-stimulated BV2 microglial cells
Park, Yong-Ki ; Lee, Kyuong-Yeol ;
The Korea Journal of Herbology, volume 22, issue 4, 2007, Pages 109~116
Objectives : In the present study, we investigated anti-inflammatory effects of chloroform fraction of Coptidis rhizoma (CR-C) on the production of inflammatory mediators such as nitric oxide (NO) and proinflammatory cytokines, tumor necrosis factor-alpha (TNF-
) and interleukin-1beta (IL-1
) in LPS-stimulated BV2 microglial cells. Methods : Copriditis rhizoma was extracted with 80% methanol, and then extracted with chloroform. BV2 cells were pre-treated with CR-C, and stimulated with LPS. The cytotoxicity was determined by MTT assay. The production of NO and cytokines was measured by Griess assay and ELISA. The mRNA expression of inducible nirtic oxide synthase (iNOS) and cytokines were determined by RT-PCR. Results : CR-C significantly inhibited the production of NO. TNF-
in a dose-dependent manner in LPS-stimulated BV2 cells. In addition, CR-C suppressed the mRNA expressions of iNOS and inflammatory cytokines induced by LPS stimulation. These results indicate that CR-C was involved in anti-inflammatory effects in activated microglia. Conclusion : The present study suggests that chloroform extract of Coptidis rhizoma can be useful as a potential anti-inflammatory agent for treatment of various neurodegenerative diseases.
Effects of Berberine on the production of inflammatory mediators from LPS-stimulated BV2 microglial cells
Park, Yong-Ki ; Jung, Hyo-Won ; Kim, Chang-Min ; Choi, Jae-Sue ; Kim, Yeong-Shik ;
The Korea Journal of Herbology, volume 22, issue 4, 2007, Pages 117~125
Objectives : Berberine, a main alkaloid component of Coptidis rhizoma, has an antimicrobial and anti-tumor activities and antiinflammatory effect. In the present study, we investigated effect of berberine on the production of inflammatory mediators such as nitric oxide(NO), prostaglandin E2(PGE2), TNF-
in LPS-stimulated BV2 microglial cells, Methods : BV2 cells were pre-treated with berberine and then stimulated with LPS. The cytotoxicity of berberine was determined by MTT assay. The NO production was measured by Griess assay. The mRNA expression and protein levels of inducible nirtic oxide synthase(iNOS) were determined by RT-PCR and Western blot. The production of PGE2 and cytokines was measured by ELISA. Results : Berberine inhibited the production of NO, PGE2 and pro inflammatory cytokines, TNF-
in a dose dependent manner in LPS-stimulated BV2 cells. In addition, berebrine greatly suppressed the mRNA expression and protein levels of iNOS and inflammatory cytokines induced by LPS stimulation. These results indicate that the post-transcriptional regulatory mechanism of iNOS and/or inflammatory cytokine gene expression by berberine is involved in its anti-inflammatory effects, respectively. Conclusion : The present study suggests that berberine can be useful as a potential anti-inflammatory agent for treatment of various neurodegenerative diseases such as Alsheimer's disease, Parkinson's disease and stroke.
Effects of Mixed Pills of Chokong (Pickled Black Soybeans) with Medicinal Herbs on Body Weight Gain and Lipid Profiles in Rats Fed High-fat Diet
Park, Chan-Sung ; Kim, Dong-Han ; Kim, Mi-Lim ; Suk, Jang-Mi ; Kim, Mi-Ryeo ;
The Korea Journal of Herbology, volume 22, issue 4, 2007, Pages 127~135
Objectives : In this study we investigated the antiobese effects of mixed pills of pickled black soybeans with herbs(herbal Chokong pill, hereafter HCKP) in rats fed high-fat diet. It was evaluated by measuring the changes of body weight, adipose tissues weight and lipid profiles in serum. Methods : Black soybeans were pickled in vinegar for 15 days to prepare Chokong, at room temperature. For treatment group, HCKP was prepared, which five kinds of medicinal herbs(Rhynchosia nulubilis, Glycyrrhiza uralensis, Zizyphus vulgaris, Atractylodes macrocephala K, Astragalus membranaceus and Cornus officinalis) were added to dried Chokong. Four groups of male Sprague-Dawley rats were fed different diets during 9 weeks: normal diet containing 5%(w/w) com oil, high-fat diet containing 10%(w/w) lard plus 5%(w/w) corn oil (HF), high-fat diet supplemented with 1%(T1) and 5%(T5) HCKP powder, respectively. Results : The T5 group had markedly lower body weight gain and weights of epididymal adipose tissue when compared with HF group. There were significant differences in visceral adipose tissue weights, serum total cholesterol and triglyceride concentrations between the HF and T5 group. Then, the efficacy of powered HCKP on body weight and lipid profiles change in rats fed high-fat diet were induced dose dependantly. Conclusion : These results suggest that the possibility of HCKP, as an antiobese functional formula, by suppression of body weight gain and improved lipid profiles.
Inhibitory effect of the extract of Catalpa ovata G. Don. on endothelial adhesion molecule expression
Choi, Byung-Min ; Chong, Myong-Soo ; Song, Ho-Joon ;
The Korea Journal of Herbology, volume 22, issue 4, 2007, Pages 137~143
Objectives : Catalpa ovata G. Don (Bignoniaceae) has been shown to possess a variety of pharmacological activities. However, the effect of Catalpa ovata G. Don on endothelial adhesion molecule expression has not been reported. Methods : To examine the effect of Catalpa ovata G. Don on the expression of adhesion molecules in human umbilical vein endothelial cells (HUVECs) stimulated with tumor necrosis factor-
), we used various methods such as Western blot analysis, reverse tranascription-polymerase chain reaction (RT-PCR), and luciferase activity assay. Results : 1. The extract of Catalpa ovata G. Don inhibited the expression of intracellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) in HUVECs stimulated with TNF-
. 2. The extract of Catalpa ovata G. Don reduced TNF-
-induced adhesion of leukocytes to HUVECs. 3. In addition, The extract of Catalpa ovata G. Don inhibited the promoter activities of ICAM-1 and VCAM-1. Conclusions : These results that Catalpa ovata G. Don may be beneficial in the treatment of inflammatory such as atherosclerosis.
The Effects of Toosendan fructus(TOF) treatment on Hematological and Cyto-pathological Alterations in Non-Bacterial Prostatitis Rat Model
Cho, Chung-Sik ; Lee, Koo-Hyen ; Chang, Sun-Kyu ; Choi, Jeong-Sik ; Kim, Cheol-Jung ;
The Korea Journal of Herbology, volume 22, issue 4, 2007, Pages 145~153
Objective : Although chronic non-bacterial prostatitis is a common disease, it is very difficult to treat effectively. Toosendan fructus(TOF) has been traditionally used in treatment of abdominal pain and parasite. In this study, we investigated the therapeutic effects and action mechanism of Toosendan fructus (TOF) in the rat model of non-bacterial prostatitis induced by castration and testosterone treatment. Method : Eight-month-old rats were treated with
-estradiol after castration for induction of experimental non-bacterial prostatitis, which is similar to human chronic prostatitis in histophatological profiles. Toosendan fructus(TOF) and testosterone were administered as an experimental specimen and a positive control, respectively. The prostates were evaluated by histopahological parameters including the epithelial score and epithelio-stromal ratio for glandular damage. Also, the prostates were observed by hematological alterations of WBC, RBC, hemoglobin, hematocrit and platelet. Results : While prostates of control rats revealed severe acinar gland atrophy and stromal proliferation, the rats treated with Toosendan fructus(TOF) showed a diminished range of the tissue damage. Epithelial score was improved in Toosendan fructus(TOF) than that of the control. The epithelio-stromal ratio was lower in Toosendan fructus(TOF) when compared to that of the control. Also, the examination of bloods were not observed hematological change. Conclusions : These findings suggest that Toosendan fructus(TOF) may protect the glandular epithelial cells and may take hematological safety. We concluded that Toosendan fructus(TOF) may could be a useful remedy agents for treating the chronic non-bacterial prostatitis.
Comparative Study on the Anti-Oxidative Effects of Achyranthis Japonicae Radix, Achyranthis Bidentatae Radix and Cyathulae Radix
Park, Jae-Sang ; Seong, Nak-Sul ; Lee, Young-Jong ;
The Korea Journal of Herbology, volume 22, issue 4, 2007, Pages 155~167
Objectives : This study was intended to compare genetic differences and anti-oxidative effects of Achyranthis Japonicae Radix, Achyranthis Bidentatae Radix and Cyathulae Radix. Methods : The genetic differences of Achyranthis Japonicae Radix, Achyranthis Bidentatae Radix and Cyathulae Radix were observed with RAPD analysis. Anti-oxidative effects of Japonicae Radix, Achyranthis Bidentatae Radix and Cyathulae Radix were investigated. Result : 1. As the result of RAPD analysis, Achyranthis Japonicae Radix was found to be genetically similar with Achyranthis Bidentatae Radix. 2. There was a little difference in the DPPH free radical scavenging activity, lipid peroxidation inhibiting activity, SOD activity of Achyranthis Japonicae Radix, Achyranthis Bidentatae Radix and Cyathulae Radix. Generally, Achyranthis Japonicae Radix was found to have stronger effect than the others. Conclusions : Achyranthis Japonicae Radix and Achyranthis Bidentatae Radix were genetically similar. Achyranthis Japonicae Radix, Achyranthis Bidentatae Radix and Cyathulae Radix all exhibited anti-oxidative action.
Study on Antioxidant Effect of Lithospermi Radix on Liver Cells Isolated from Oxidatively Stressed Rat
Kim, Sung-Hyum ; Park, Wan-Su ; Seong, Nak-Sull ; Lee, Young-Jong ;
The Korea Journal of Herbology, volume 22, issue 4, 2007, Pages 169~176
Objective : This study purposed to investigate the anti-oxidative effect of Lithospermi Radix (root of Lithospermum erythrorhizon S.) on liver cells isolated from oxidatvely stressed rat by AAPH. Method : We investigate effects of Lithospermi Radix(LR) and its fractions on normal liver cells' proliferation. And the amounts of SOD, GSH, catalase, NO, MDA production by liver cells isolated from the oxidatively stressed rat by AAPH also were measured after incubation with various fractions of LR extraction. Results : LR and its fracitons showed no toxicity on the normal liver cells from rat. LR and its fracitons increased the activity of SOD and reduced the amounts of NO and MDA in the liver cells from the oxidatively stressed rat. Conclusion : Lithospermi Radix could be supposed to have antioxidant effect on liver cells with no toxicity.
The Comparative Study of the Effects of Fructificatio Inonoti Obliqui Aqueous Extract according to the Extraction Temperature(I) -Anti-oxidative effect-
Park, Kyu-Cheon ; Kil, Ki-Jung ; Lee, Young-Jong ;
The Korea Journal of Herbology, volume 22, issue 4, 2007, Pages 177~185
Objectives : The present study purposed to compare the anti-oxidative effect of Fructificatio Inonoti Obliqui aqueous extract according to extraction temperature. Methods : We measured the total phenol content and anti-oxidative activity of Fructificatio Inonoti Obliqui total extract and
low-temperature leachate. Results : Anti-oxidative activity of Fructificatio Inonoti Obliqui total extract and
low-temperature leachate was different according to extraction temperature, concentration, and antioxidant effect measuring method. In all experiments, the total extract showed higher antioxidant than n-BuOH fraction. The total phenol content was higher in Fructificatio Inonoti Obliqui total extract than
low-temperature leachate. Conclusions : Both Fructificatio Inonoti Obliqui total extract and Fructificatio Inonoti Obliqui
low-temperature leachate have significant anti-oxidative effect.
The Comparative Study of the Effects of Fructificatio Inonoti Obliqui Aqueous Extract according to the Extraction Temperature(II) -Anti-oxidativy Activity, anti inflammatory effect and cancer cell multiplication inhibition effect-
Park, Kyu-Cheon ; Han, Hyo-Sang ; Lee, Young-Jong ;
The Korea Journal of Herbology, volume 22, issue 4, 2007, Pages 187~199
Objectives : The present study purposed to compare the antioxidant effect, anti inflammatory effect and cancer cell multiplication inhibition effect of Fructificatio Inonoti Obliqui aqueous extract according to extraction temperature. Methods : We medicated animal models, which had experimental oxidation, with Fructificatio Inonoti Obliqui total extract and
low temperature leachate, and performed hematological analysis and blood chemical analysis with measuring SOD, GSH, catalase, NO and MDA content in the liver. In addition, we made comparative observation of anti inflammatory effect and anti-cancer effect. Results : Compared to the control group, both the group medicated with Fructificatio Inonoti Obliqui total extract and with
low-temperature leachate were found to decrease the number of thrombocytes in blood plasma and NO content while to increase SOD activity and catalase activity significantly. Both groups also showed anti-inflammatory effect against THP-1 cells and a multiplication inhibition effect against liver cancer cells and stomach cancer cells significantly. Conclusions : Both Fructificatio Inonoti Obliqui total extract and Fructificatio Inonoti Obliqui
low-temperature leachate have significant antioxidant effect, anti inflammatory effect and anti cancer effect.
A Study on the Anti-oxidative Effects of the Araliae Elatae Cortex
Kim, In-Soo ; Seong, Nak-Sull ; Lee, Young-Jong ;
The Korea Journal of Herbology, volume 22, issue 4, 2007, Pages 201~212
Objectives : The trunk-bark and the root of A. elata were compared with each other in terms of their anti-oxidative activities. Methods : Using the trunk-bark and the root of A. elata, their anti-oxidative activities were examined through the in vitro and in vivo experiments. Results : The trunk-bark of A. elata in vitro had weaker anti-oxidative activities than its root. However, the trunk-bark of A. elata in vivo had stronger anti-oxidative activities than the root in the rats which was oxidatively stressed by alcohol. Conclusion : These results suggested that the trunk-bark of A. elata was also useful as a medicine.
Effects of Fructus Foeniculi extract on recovering liver function
Lee, Jang-Cheon ; Lee, Eun ; Oh, Hwang ; Yoon, Ho-Suck ; Ha, Tae-Kwang ; Hong, Eun-Hee ; Lee, Young-Cheol ;
The Korea Journal of Herbology, volume 22, issue 4, 2007, Pages 213~218
Objectives : Effects of Fructus Foeniculi extract on liver function were investigated in carbon tetrachloride(CCl4) intoxicated rats. Methods : Thirty two male Sprague-Dawley rats with mean weight of
were used in these experiments and housed with food and water ad libitum. Fructus Foeniculi extract was administerd at dose 100mg/kg/day and 200mg/kg/day p.o. for 2 weeks after that CCl4 was treated 3 times at dose of 2.5ml/kg, p.o. in alternate day basis. Then serum AFP(
-Fetoprotein), Total protein, Albumin, Triglyceride, Total cholesterol concentrations and ALP (Alkaline phosphatase), AST(Aspartate Aminotransferase), ALT(Alanine Aminotransferase),
-Glutamyl transferase), LDH(Lactate Dehydrogenase) activities were determined with commercial kit by autoanalyzer. Results : Plasma
-fetoprotein and total protein concentration showed a tendency to decrease in Fructus Foeniculi extract-treated groups. However, plasma albumin concentration showed no significant differences in all treatment groups. Activity of plasma aspartate aminotransferase and alanine aminotransferase in Fructus Foeniculi extract-treated groups showed a lower value than that of control group. Alkaline phosphatase and lactate dehydrogenase activities showed a tendency to decrease in Fructus Foeniculi treated groups. However,
-glutamyl transferase activity showed no significant difference in all treated groups. Concentration of plasma triglyceride and total cholesterol showed a high level in CCl4 intoxicated rats but not in Fructus Foeniculi treated groups. Conclusion : Reviewing these experimental results, it appears that Fructus Foeniculi extract have recovering effect against liver injury.
Anti-aging Effects Saccharomyces Fermented Modified Kyungohkgo Extract on Skin
Choi, Jae-Hwan ; Kim, Hyoung-Man ; Song, Young-Sook ; Park, Sun-Gyoo ; Kim, Jin-Jun ; Lee, Cheon-Koo ;
The Korea Journal of Herbology, volume 22, issue 4, 2007, Pages 219~225
Objectives : We investigated the anti-aging effects on skin with Saccharomyces fermented modified Kyungohkgo extract (SFKE). Methods : Fermented modified Kyungohkgo extracts by 14 different kinds of microorganism were prepared and most effective Saccharomyces fermented modified Kyungohkgo extract among these was selected for anti-aging agent. We measured various effects related to skin such as scavenging activity against free radical, cell proliferation, collagen synthesis and toxicity of SFKE were evaluated and compared with modified Kyungohkgo extract (KE). Results : Free-radical scavenging activity and cell proliferating ratio of SFKE were 2 and 8 times higher than those of KE. The SFKE could significantly increase the collagen synthetic ratio compared with KE treated group. SFKE showed no toxicity at all tested concentrations. Conclusions : The results of our study propose that SFKE have good anti-aging effects on skin.
Physiological Effects of the Cosmetic Product Containing of Saccharomyces Fermented Modified Kyungohkgo Extract on Human Skin
Choi, Jae-Hwan ; Kim, Hyoung-Man ; Song, Young-Sook ; Park, Sun-Gyoo ; Kim, Jin-Jun ; Lee, Cheon-Koo ;
The Korea Journal of Herbology, volume 22, issue 4, 2007, Pages 227~232
Objectives : We investigated the physiological effects of the cosmetic products with Saccharomyces fermented modified Kyungohkgo extract (SFKE) for 6 weeks on human skin by using non-invasive instruments. Methods : We measured physiological effects such as a skin moisturizing effect, an elasticity and an evenness on the skin of older than 40 years volunteers(female, n=25) who were applied with the cosmetic products containing SFKE for 6 weeks. Results : We observed the physiological effect after using the cosmetic products for 6 weeks. After using the cosmetic product, the skin moisture contents were increased in human skin. The skin elasticity were not only increased in face skin through using the product, but also evenness of skin were improved. Conclusions : We concluded that the cosmetic products containing the SFKE had and physiological effects on the human face skin.
Effect of Scutellariae Radix on Expression of Cytokines and Chemokines Levels in Human Mast Cells (HMC-1)
Leem, Kang-Hyun ; Kim, Myung-Gyou ; Lee, Se-Na ; Kim, Ji-Woo ; Lee, Pyeong-Jae ;
The Korea Journal of Herbology, volume 22, issue 4, 2007, Pages 233~238
Objectives : Scutellariae Radix (Hwanggeum in Korean) is the root of Scutellaria baicalensis Georgi. Scutellariae Radix is well known to be used as a medicine for common cold, upper respiratory infections, and to strengthen and regulate the immune system and anemia etc. Little is understood about the roles of Scutellariae Radix in the cytokine and chemokine secretion by immune cells. This study was designed to find out the effects of Scutellariae Radix on the cytokine and chemokine secretion in human mast cells (HMC-1). Methods : We treated hwanggeum according to consistency on HMC-1 and measured cytokines and chemokines levels using flow cytometry CBA system. Results : In hwanggeum treated group, the expression of interferon-inducible protein 10 (IP-10), monocyte chemoattractant protein-1 (MCP-1), chemokine (C-X-C motif) ligand 9 (CXCL9, MIG), interleukin 8 (IL-8), interleukin 2 (IL-2), interleukin 4 (IL-4), interleukin 5 (IL-5), interleukin 10 (IL-10), and interferon
) were decreased significantly. Conclusion : These results suggest that hwanggeum may support some of immune diseases by means of amiliorating some chemokines or cytokines such as IP-10, MCP-1, MIG, IL-8, IL-2, IL-4, IL-5, IL-10, and IFN-
Effects of Salviae miltiorrhizae Radix (SMR) on Serum Lipid Level in Hyperlipidemic Rats
Kim, Hyeong-Cheol ; Kim, Hyung-Woo ; Kim, Yong-Seong ; Lee, Jang-Sik ; Kwon, Jeong-Nam ; Kim, Young-Gyun ; Cho, Su-In ;
The Korea Journal of Herbology, volume 22, issue 4, 2007, Pages 239~245
Objective : Salviae miltiorrhizae Radix (SMR) has widely used to treat patients with cardiovascular diseases such as coronary arteriosclerosis, angina pectoris and hyperlipidemia. This study was designed to investigate the effects of SMR on changes in serum cholesterol and protective effects on liver tissue damage in Hyperlipidemic rats. Methods : The present author investigated changes in serum glucose, cholesterols, AST/ALT and histopathological changes of liver tissue by oral administration of SMR in Rats. Results : In this study, body weights of hyperlipidemic rats induced high fat diet did not changed, and treatment with SMR did not affect body weights in hyperlipidemic rats. For experimental period, Food and Water uptake in SMR administered group were the same as those in hyperlipidemic control group. In this experiment, treatment with SMR decreased total cholesterol and AST in serum which elevated by high fat diet respectively. In addition, SMR administration protected liver tissue from damage induced by induction of hyperlipidemia. Conclusions: These results suggest that SMR is useful to treat patients with disease related to cardiovascular diseases including hyperlipidemia, because SMR can decrease cholesterol and AST in serum and also have non-specific protective effect on tissues including liver.
The study on preventive effects of Fructus Amomi on miscarriage in Dongeui-bogam
Kim, Hyung-Woo ; Shin, Woo-Cheol ; Sim, Seung-Yong ; Sim, Chang-Min ; Yeo, Seon-Mi ; Cho, Su-In ;
The Korea Journal of Herbology, volume 22, issue 4, 2007, Pages 247~252
Objectives : Fructus Amomi has been widely used to treat patients with anorexia and dyspepsia induced by moisture or cold. In addition, it is known to have preventive effects on miscarriage. But, this preventive effect of Fructus Amomi has controversial point. For this reason, this study was designed to investigate the preventive effects of Fructus Amomi on miscarriage in the framework of usage. Methods : We investigated the preventive effects of Fructus Amomi on miscarriage in terms of checking frequency in use of Fructus Amomi in Dongeui-bogam. Results : Fructus Amomi was used by 192 times in Dongeuh-bogam. it was used by 36 times in major drug, the others used in complementary drug. 14 remedies were used to treat patients with gynecologic diseases. In these remedies, 7 remedies were related to preventive effects of Fructus Amomi on miscarriage. In thses 7 remedies, 6 were used with samul-tang or Radix Scutellariae and Rizoma Atractylodis Macrocephalae. Conclusions : These results suggest the possibility that preventive effects of Fructus Amomi on miscarriage is not involved in direct efficacy, which was explained with gimi-ron, but in usage of the herbal medicine.
Microarray-Based Gene Expression Profiling to Elucidate the Effectiveness of Woowhangchongshim-won on ICH Model in Rats
Kim, Hyung-Woo ; Cho, Su-Jin ; Kim, Bu-Yeo ; Jeong, Byeong-Han ; Bong, Sung-Jeon ; Kim, Yong-Seong ; Lee, Jang-Sik ; Kwon, Jeong-Nam ; Kim, Young-Kyun ; Cho, Su-In ;
The Korea Journal of Herbology, volume 22, issue 4, 2007, Pages 253~260
Objectives : Intracerebral hemorrhage (ICH) is characterized by breakdown of blood vessels within the brain parenchyma. Fundamental therapeutic strategies for ICH, particularly those aimed at neuroprotection, have to be established. So in this experiment, the effects of Woowhangchongshim-won, a traditional prescription formula for treating Cerebral Apoplexy in Asian countries, were investigated. Methods : After intraperitoneal injection of chloralhydrate, rats were placed in a stereotaxic frame. ICH was induced by injection of 1 U collagenase type IV and drug was administered orally for 10 days. The molecular profile of cerebral hemorrhage in rat brain tissue was measured using micro array technique to identify up- or down- regulated genes in brain tissue. These genes induced by brain damage were mainly concerned with general metabolic process such as primary metabolic process, cellular metabolic process, macromolecule metabolic process, and biosynthetic process. Results : The number of genes increased in control and not-changed in experiment was 374, and decreased in control and not-changed in experiment was 527. We are concerned with genes that can be recovered by treatment with medicine, it is especially interesting to above types of genes. Conclusions : Upon medicine treatment to the rat having cerebral hemorrhage, expressions of some genes were restored to normal level. Further analysis using protein interaction database identified some key molecules that can be used for elucidation of therapeutical mechanism of medicine in future.
Studies on the Changes of Oligosaccharide Contents in Rehmanniae Radix preparata According to Various Processing methods
Choi, Ho-Young ; Kwon, Seung-Ro ; Kim, Hyo-Geun ; Ham, In-Hye ; Lee, Jae-Jun ; Lee, Je-Hyeon ; Hong, Seon-Pyo ; Kim, Do-Hoon ;
The Korea Journal of Herbology, volume 22, issue 4, 2007, Pages 261~270
Objective : The 5-HMF was not index material suitable to do the quality control of Rehmanniae Radix Preparata. In this study, We estimated the changes of oligosaccharide contents in Rehmanniae Radix Preparata using high-performance anion-exchange chromatography with pulsed amperometric detection(HPAEC-PAD). Methods : The analysis of oligosaccharide was conducted by HPAEC-PAD with Carbopac PA1,
, 5um, and Carbopac PA1 guard column. Column temperature was kept at
. Elution was carried out at 1000
with 70mM NaOH and the injection volume was
. Each component was detected by PAD. Results : Nine constituents were found from merchandising Rehmanniae Radix Preparata(MR), while seven constituents were found in various processed Rehmanniae Radix Preparata. Not all constituents were defined but stachyose and raffinose were found in all cases. And The most common constituents of Rehmanniae radix was stachyose. In the course of processing, most of stachyose and raffinose were decreased. Stachyose was decreased slowly in the course of processing with rice wine(RR), amomi and rice wine(AR), and crataegi and rice wine(CR). However stachyose was decreased rapidly in the course of processing with fresh rehmannia juice(FR). The method with crataegi and rice wine(CR) showed the smallest decrease of stachyose. And processing method with crataegi and rice wine(CR) showed the most abundant amount for stachyose after the nineth processing. Conclusion : The changes of oligosaccharides in the course of processing were a very important direct barometers to do the quality control and set up a standard of Rehmanniae Radix Preparata.
Analysis of the content of essential oils from Ssajuari and Sajabalssuk according to storage period
Choi, Ho-Young ; Ham, In-Hye ; Choi, Cheol-Han ; Bang, Chan-Sung ; Lee, Byung-Hee ; Cheng, Hae-Gon ; Bu, Young-Min ; Kim, Ho-Cheol ;
The Korea Journal of Herbology, volume 22, issue 4, 2007, Pages 271~277
Objective : The content of essential oils from Ssajuari and Sajabalssuk was analyzed based on storage period. A total of eight samples old Ssajuari (harvested in 2000, 2001, 2002, 2003, and 2004), fresh Ssajuari (harvested in 2004), old Sajabalssuk (harvested in 2002), and fresh Sajabalssuk (harvested in 2004) was analyzed. Methods : The analysis of the content of essential oils from Ssajuari and Sajabalssuk was conducted by GC/MS. Results : The main essential oils of Ssajuari were
-thujone and 1.8-cineole. The
-thujone and 1,8-cineole were increased but the caryophyllene oxide was decreased. in accordance with storage period. The main essential oils of fresh Sajabalssuk were trans-
-caryophyllene, terpinen-4-ol. The main essential oils of old Sajabalssuk was
-thujone and 1,8-cineole like Ssajuari. But 1,8-cineole was higher than that of Ssajuari samlpes. Conclusion : All samples had trans-sabinene hydrate, 1,8-cineole, terpinen-4-ol and caryophyllene oxide. According to stored year,
-thujone were increased but terpinen-4-ol, caryophyllene oxide were decreased.
Effects of Cynanchum spp. on the hyperlipidemia in rats induced by Triton WR-1339
Choi, Ho-Young ; Ham, In-Hye ; Lee, Ju-Yeong ; Yoon, Ye-Jin ; Yang, Gab-Sik ; Tae, Jin ; Bu, Young-Min ; Kim, Ho-Cheol ;
The Korea Journal of Herbology, volume 22, issue 4, 2007, Pages 279~286
Objective : In order to compare of hyperlipidemia effect of C. wilfordii, C. auriculatum in korea and C. auriculatum in China, it was performed on the hyperlipidemia in rats induced by Triton WR-1339. Method : The 70% MeOH extracts from dried root of C. wilfordii and C. auriculatum in korea and C. auriculatum in china were fractionated into H2O layer and CHCl3 layer. The level of total cholesterol, triglyceride, HDL-cholesterol, LDL-cholesterol, AST and ALT in serum were used to observe the effectiveness of C. wilfordii korean C. auriculatum and chinese C. auriculatum. Results : In the hyperlipidemic rats induced by Triton WR-1339, C. wilfordii and korean C. auriculatum significantly decreased total cholesterol, triglyceride, otherwise significantly increased HDL. And C. wilfordii and chinese C. auriculatum significantly decreased AST and ALT. Conclusion : This study indicate that C. wilfordii korean C. auriculatum and chinese C. auriculatum were effective in the hyperlipidemic rats induced by Triton WR-1339. According to the results, C. wilfordii korean C. auriculatum and chinese C. auriculatum need to considered for the application in hyperlipidemia.
A study on Genotoxicity Test of Hyeong-gae-yeon-gyo-tang extract
Jee, Seon-Young ; Hwang, Sun-Yi ; Lee, Jong-Rok ; Kim, Sang-Chan ;
The Korea Journal of Herbology, volume 22, issue 4, 2007, Pages 287~300
Objectives : The genotoxicity of extract of "Hyeonggaeyeongyotang", a polyherbal formula has been used as a tonic agents in oriental medicine was tested. Methods : Extract of "Hyeonggaeyeongyotang" was tested by In Vitro Chromosome Aberration Test, Bacterial Reverse Mutation Assay and Micronucleus test according to OECD Guidelines and KFDA Guidelines [2005-60]. Results : The obtained results were as follows: 1. Chromosome Aberration Test: No significant changes in the number of aberrant metaphases having structural and number of aberrations were detected in all concentrations of "Hyeonggaeyeongyotang" extracts treated in this study. 2. Bacterial Reverse Mutation Assay: No significant increases in the number of revertant colonies compared to its negative control were detected in all concentrations of "Hyeonggaeyeongyotang" extracts treated in this study against all 5 strains except for
treated group where significantly decreases in colony numbers were detected agains all five strains used in this study as pharmacological effects not genotoxicity. 3. Micronucleus test: No significant changes in the number of micronucleated polychromatic erythrocytes among 2000 polychromatic erythrocytes compared to negative control were detected in all "Hyeonggaeyeongyotang" extracts-dosing groups tested. Conclusions : From above-mentioned results, it is concluded that "Hyeonggaeyeongyotang" extracts have not any genotoxicity against In Vitro Chromosome Aberration Test, Bacterial Reverse Mutation Assay and Micronucleus test.