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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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The Korea Journal of Herbology
Journal Basic Information
Journal DOI :
The Korea Association of Herbology
Editor in Chief :
Volume & Issues
Volume 24, Issue 4 - Dec 2009
Volume 24, Issue 3 - Sep 2009
Volume 24, Issue 2 - Jun 2009
Volume 24, Issue 1 - Mar 2009
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Studies on the Storage Characteristics of Angelica dahuricae Radix, Glehnia littoralis Radix Treated with Gamma-irradiation
Kim, Soo-Min ; Kim, Eun-Ju ;
The Korea Journal of Herbology, volume 24, issue 1, 2009, Pages 1~7
Objectives : The purpose of this study is to investigate on storage characteristics of Herbal materials treated with Gamma-irradiation(10 KGy). Methods : This experiments were carried out by field survey and storage characteristics were carried out by physicochemical determination. Results : Antimicrobial activity of oriental medicine materials(Angelica dahurica Radix, Glehnia littoralis Radix) were examined, together with investigation of effects on gamma-irradiation. Oriental medicine materials contaminated in microbial cell were tend to be pinhole in packaging materials(polypropylene) by bug and Larva. At the same time, PVDC (polyvinylldichloride) package also was founded in bug and Larva by microbial contamination during storage in room temperature. Conclusions : In conclusion, it is very desirable to dose gamma-irradiation(10 KGy) in these oriental medicine materials in order to prevent microbial activity.
Studies on Storage Characteristics of Perilla Perfrutescens var. Acuta, Mentha Arvensis L. var. Piperascens Malinvaud According to Packaging Method
Kim, Soo-Min ; Kim, Eun-Ju ;
The Korea Journal of Herbology, volume 24, issue 1, 2009, Pages 9~14
Objectives : The purpose of this study is to investigate on storage characteristics of flavouring oriental medicine materials according to Packaging method(Aluminum package, PP). Methods : This experiments were carried out by field survey and storage characteristics were carried out by physicochemical determination. Results : Flavouring oriental medicine materials were used to in aluminum package to keep original flavour in Japan and Chinese by field and study survey. In view of this survey results, it is very desirable to use zipper Aluminum package in flavouring oriental medicine materials(Perilla perfrutescens var. acuta, Mentha arvensis L. var. piperascens malinvaud). Conclusions : This study results revealed that Aluminum package were superior to any other package method on the basis of keeping original flavour and to reduce microbial contamination in oriental medicine materials.
Effect of Water Extract from Hordeum vulgare L. with Medicinal Herb on Plasma Lipid Status and Glucose in Rats Fed High Fat Diet
Park, Hyun-Suk ; Yang, Kyung-Mi ; Jung, Ji-Wook ;
The Korea Journal of Herbology, volume 24, issue 1, 2009, Pages 15~21
Objectives : This study was designed to investigate the effect of water extract from Paeonia Lactiflora Pasll. with medicinal herb on plasma lipid and glucose in rats fed high fat diet. Methods : Experimental groups were divided into control diet group(C), high fat diet group(HF), high fat diet and 5% water extracts from Paeonia Lactiflora Pasll. with medicinal herb group(HFE I), high fat diet and 10% water extract from Paeonia Lactiflora Pasll. with medicinal herb group(HFE II). Weight gain, food intake, food efficiency, plasma lipid concentration and glucose were determined for 10 weeks during high fat diet. Results : In this experiment, body weight and food efficiency were significantly increase in high fat diet. High fat diet group showed elevated levels of total cholesterol, LDL-cholesterol and free fatty acid. Consumption of 10% water extract Paeonia Lactiflora Pasll. with medicinal herb lowers level of total cholesterol, LDL-cholesterol and free fatty acid. But levels of plasma triglyceride, HDL-cholesterol and glucose did not affect by high fat diet and water extract Paeonia Lactiflora Pasll. with medicinal herb Conclusions : In these results, water extract Paeonia Lactiflora Pasll. with medicinal herb in high fat diet group decreased plasma total cholesterol, LDL-cholesterol and free fatty acid. Thus the Paeonia Lactiflora Pasll. with medicinal herb might be useful in the treatment of hyperlipemia.
Study on the Antioxidant and Anticancer Effects of Extract of Stamens of Nelumbo nucifera and Kaempferol
Han, Du-Seok ; Jeon, Sung-Woo ; Kim, Hyun-Jin ;
The Korea Journal of Herbology, volume 24, issue 1, 2009, Pages 23~33
Objectives : The present study was performed to clarify the antioxidant and anticancer effects of extract of stamens of Nelumbo nucifera and Kaempferol. Methods : Antioxidant effect was measured by DPPH-radical scavenging activity for electron donating ability (EDA), superoxide dismutase (SOD)-like activity for SOD and lipid peroxidation. Anticancer effect was assessed by MTT absorbance for cytotoxicity. Results : Vitamin E, Kaempferol and ethyl acetate extract of stamens of Nelumbo nucifera increased SOD-like activity and DPPH-radical scavenging activity dose-dependently. On the contrary, lipid peroxidation was time-dependently decreased. Furthermore, Kaempferol and ethyl acetate extract of stamens of Nelumbo nucifera significantly decreased the growth rate of C6 glioma cells. Conclusions : These results suggest that ethyl acetate extract of stamens of Nelumbo nucifera may be a putative antioxidant or anticancer substance.
Anti-oxidant Effects of Portulaca oleracea L. on HCl-ethanol Induced Gastritis in Rats
Kim, Chae-Hyun ;
The Korea Journal of Herbology, volume 24, issue 1, 2009, Pages 35~40
Objectives : The purpose of this study is to evaluate whether or not a pretreatment with Portulaca oleracea has an antioxidant effect in HCl-ethanol induced gastric mucosal damage. Methods : We elucidated the level of reactive oxygen species (ROS), lipid peroxidation, and two important constituents of antioxidant defense such as superoxide dismutase (SOD), glutathione (GSH) in these effects. Results : The oral administration of crude extract from P. oleracea attenuated the gastritic lesion area, submucosal edema and hemorrhage, and mucosal necrosis induced by HCl-ethanol. The MDA levels of control group were higher than those in the rats given the P. oleracea pretreatment. While the GSH levels of control were decreased, the GSH activity on the gastric mucosal layer maintain normal level in rats given the Portulaca oleracea pretreatment before HCl-ethanol induced gastritis significantly increased. However, the SOD activites were not altered by P. oleracea. Conclusions : The administration of Portulaca oleracea have a protective antioxidant effect against the gastric lesion induced by HCl-ethanol and may therefore be a promising drug for gastritis and gastric ulcer.
The Inhibitory Effects of Portulaca oleracea L. on HCl-ethanol Induced Gastritis in Rats
Kim, Chae-Hyun ;
The Korea Journal of Herbology, volume 24, issue 1, 2009, Pages 41~47
Objectives : The objective of this study was to examine the effects of P. oleracea into the HCl-ethanol induced gastritis in rats, and to isolate and determine the chemical compounds from P. oleracea. Methods : The rats were orally administered with crude extract or fractions or isolated compounds of P. oleracea 30 mins before the induction of gastric lesion by oral administration of HCl-ethanol. The gastric lesional area was measured using pixel counting software. Then the chemical compounds from P. oleracea was isolated and determined by LC-MS and NMR. Results : The inhibition effect of oral administration of crude extract of P. oleracea at a dose of 500 mg/kg in HCl-ethanol induced gastritis was similar to cimetidine. Then, aqueous fraction at a dose of 240 mg/kg exhibited the effects similar to cimetidine. Then, the aqueous fraction was further separated by MPLC and yielded four sub fractions. Among those sub fractions, agent II at a dose of 40 mg/kg possessed the strongest effect in the HCl-ethanol induced gastritis. The water fraction yielded-Uridine, Adenosine, Guanosine, which were characterized by Mass, 1H-NMR, 13C-NMR. Conclusions : This study suggest that a P. oleracea and its compounds showed potent efficacy on the development of HCl-ethanol induced gastritis. Thus, P. olaracea can be a potential natural resource for the management of gastritis although the mechanism of action involved in the treatment remains to be explored.
The Antimicrobial Activity of Gwankeibujalijung-tang Against Streptococcus pneumoniae 59 Isolated from the Mouth of a Common Cold Patient
Lee, Sang-Yun ; Seo, Bu-Il ; Park, Ji-Ha ; Roh, Seong-Soo ; Lee, Eun-Sook ; Kim, Yong-Hyun ;
The Korea Journal of Herbology, volume 24, issue 1, 2009, Pages 49~57
Objectives：I want to examine the antimicrobial activity of Gwankeibujalijung-tang against Streptococcus pneumoniae 59 isolated from the mouth of a common cold patient. Methods： Antimicrobial activity was assayed through the hot water extract from Gwankeibujalijung-tang against Streptococcus pneumoniae 59 isolated from the mouth of a common cold patient. Results： The size of inhibition zone of Gwankeibujalijung-tang extract was 9.83
0.11 mm. The optimal pH and temperature for the growth of isolated Streptococcus pneumoniae 59 were 7.0 and 37
, respectively. The minimum inhibitory concentration of Gwankeibujalijung-tang extract was 12
and the antimicrobial activity of Gwankeibujalijung-tang extract was not destroyed by the heat (121
for 15 min) and not affected by pH. Conclusions： Reviewing this experimental result, it appeared that Gwankeibujalijung-tang had efficacy against Streptococcus pneumoniae 59 isolated from the mouth of a common cold patient.
Effect of Mantidis
and Mori Fructus On treatment of Osteoporosis In Ovariectomized Rats
Lee, Jae-Woo ; Seo, Bu-Il ; Park, Ji-Ha ; Roh, Seong-Soo ; Kim, Yong-Hyun ; Kim, Mi-Ryeo ;
The Korea Journal of Herbology, volume 24, issue 1, 2009, Pages 59~71
Objectives：The present study has been undertaken to investigate the effects of Mantidis
and Mori Fructus on treatment of osteoporosis in ovariectomized rats. Methods： In this experiment, the rats were ovariectomized. Rats were administered by Mantidis
and Mori Fructus. The levels of bone mineral density, osteocalcin.ALP.calcium.phosphorus in serum, calcium. phosphorus.deoxypyridinoline in urine and calcium.phosphorus.ash weight in bone were measured. Results： 1. The levels of femoral and fibula-tibial bone mineral density were significantly increased in comparison with OVX group at 4, 8 weeks in Mantidis
group. And the levels of femoral and fibula-tibial bone mineral density were significantly increased in comparison with OVX group at 8 weeks in Mori Fructus group. 2. The levels of serum osteoclacin and ALP showed significant decrease in comparison with OVX group at 4, 8 weeks in Mantidis
and Mori Fructus group. The levels of serum calcium showed significant decrease in comparison with OVX group at 4 weeks in Mantidis
and Mori Fructus group. The levels of serum phosphorus showed significant decrease in comparison with OVX group at 4, 8 weeks in Mantidis
and Mori Fructus group. 3. The levels of urine calcium, phosphorus and deoxypyridinoline showed significant decrease in comparison with OVX group in Mantidis
and Mori Fructus group. 4. The levels of fibula-tibial calcium and phosphorus showed significant increase in comparison with OVX group in Mantidis
group and Mori Fructus group. The levels of femoral calcium and phosphorus showed significant increase in comparison with OVX group in Mori Fructus group. The levels of femoral and fibula-tibial ash weight showed significant increase in comparison with OVX group in Mantidis
group and Mori Fructus group. Conclusions： Reviewing these experimetal results, it appeared that Mantidis
and Mori Fructus had efficacy on treatment of osteoporosis.
A Therapeutic Effect of Pinellia Ternata via the Increase of CD4+CD25+ Regulatory T Cells and the Suppression of CD3+CCR3+ Cellular Infiltration During Allergic Airway Inflammation
Lee, Young-Cheol ;
The Korea Journal of Herbology, volume 24, issue 1, 2009, Pages 73~78
Objectives : In this study, we studied the effect of Pinellia Ternata (PT) on regulatory T cells and CD3+CCR3+ Th2 cells number in asthma model mice. Methods : All mice were immunized on two different days (21 days and 7 days before inhalational exposure) by i.p. injections of 0.2
alum-precipitated Ag containing 100
of OVA bound to 4 mg of aluminum hydroxide in PBS. Seven days after the second sensitization, mice were exposed to aerosolized ovalbumin for 30 min/day on 3 days/week for 12 weeks(at a flow rate of 250 L/min, 2.5％ ovalbumin in normal saline) and PT (400, 200 mg/kg) were orally administered 3 times a week for 8 weeks. After C57BL/6 mice were orally given of PT, the percentages, cell numbers, phenotype and function of CD4+CD25+Treg cells were determined by flow cytometry. Results : The cell numbers of CD4+CD25+Treg cell subsets were markedly increased in PT treated mice as reported. However, PT significantly reduced the CD3+CCR3+ Th2 cells in PBMC and lung of mice. Conclusions : These results indicate that PT has a deep inhibitory effect on asthma model mice by increase the number of regulatory T cells, and by reducing CD3+CCR3+ Th2 cells.
Effects of Piperis Longi Fructus on Regulatory T Cells Number, IgE, Histamine Production in Asthma Model Mice and Th1/Th2 Cytokine Balance in vitro
Lee, Young-Cheol ; Kim, Seung-Hyung ;
The Korea Journal of Herbology, volume 24, issue 1, 2009, Pages 79~88
Objectives : It has been recently shown that Piperis Longi Fructus (PLF) is involved in the reduction of eosinophil recruitment and production of Th2 cytokines in vivo. However, the main therapeutic mechanisms of PLF remains a matter of considerable debate. To investigate the therapeutic mechanisms of PLF, we examined the influence of PLF on regulatory T cells number, IgE, histamine production in vivo and Th1/Th2 cytokine balance in vitro. Methods : All mice were immunized on two different days (21 days and 7 days before inhalational exposure) by i.p. injections of 0.2
alum-precipitated Ag containing 100
of OVA bound to 4 mg of aluminum hydroxide in PBS. Seven days after the second sensitization, mice were exposed to aerosolized ovalbumin for 30 min/day on 3 days/week for 12 weeks(at a flow rate of 250 L/min, 2.5％ ovalbumin in normal saline) and PLF (150 mg/kg) were orally administered 3 times a week for 8 weeks. Splenocytes from C57BL/6 mice at 8 weeks of age were stimulated with anti-CD3 (1 mg/ml) plus anti-CD28 (1 mg/ml) antibody for 48hrs. IL-4 and IFN-
in the culture supernatants were measured by ELISA Results : The suppressive effects of PLF on asthma model were demonstrated by the increase the number of regulatory T cells and by reducing IgE, histamine production in vivo and modulation of Th1/Th2 cytokine balance. Conclusions : These results indicate that PLF has a deep inhibitory effects on asthma model mice by increase the number of regulatory T cells, and by reducing IgE, histamine production.
Effect of Red Ginseng with Processed Sulfur Extracts on Serum Lipids Concentration and Metabolic Variables in Diabetic Rats
Han, Hyun-Jung ; Kim, Hae-Ja ; Chong, Myong-Soo ; Cho, Hwa-Eun ; Choi, Yun-Hee ; Lee, Ki-Nam ;
The Korea Journal of Herbology, volume 24, issue 1, 2009, Pages 89~98
Objectives : The purpose of this study was to evaluate effects of processed sulfur with red ginseng on streptozotocin(STZ) induced diabetic rats for expansion of processed sulfur internal application. Methods : We prepared red ginseng with non processed sulfur extracts(GS), red ginseng with processed sulfur I extracts(GPS I) and red ginseng with processed sulfur II extracts(GPS II). In the present study, we examined about contents of crdue saponin, antioxidant activity,
-glucosidase inhibitory activity, and effects of STZ induced diabetic rats. Results : Contents of crude saponin increased by processed sulfur, and GPS II was shown highest contents in crude saponin and sulfur compared with another groups. Electron donating ability of GPS II was shown highest activity compared with GS and GPS I, SOD-like activity showed same tendency as electron donating ability at 1
concentration. Inhibitory activity of
-glucosidase was approximately same level in acarbose and GPS II. Blood glucose level of GPS II group was decreased 18.34% compared with DC(diabetes control) group and maintained stability range in glucose level. but GS and GPS I showed high level compared to GPS II. Serum triglycerides concentration also showed lowest level in GPS II. The activity of ALT, AST and ALP was shown high level in diabetic induced groups, and lowest level in GPS II. Creatinine was shown non-significantly difference in each groups and GPS II was shown lowest level in BUN. Conclusions : These results suggested that processed sulfur with red ginseng have improvement effects on diabetes and internal application of processed sulfur with red ginseng have no specific toxicity in liver and kidney.
Effects of the Coptidis Rhizoma Extract on the Membranous Nephropathy induced by Cationic Bovine Serum Albumin in Mice
Chae, Eun-Young ; Cho, Chung-Sik ; Kim, Cheol-Jung ;
The Korea Journal of Herbology, volume 24, issue 1, 2009, Pages 99~110
Objectives : The current treatment regimens for patients with nephrotic syndrome due to membranous nephropathy(MN) are based on steroids or immunosuppressive therapy with the aim of reducing proteinuria and improving outcome. Although these treatments attenuate the deterioration of renal function in MN patients, it has been suggested that all are burdened by significant toxicity. Therefore, more specific and less toxic therapies are needed. This study was to evaluate the effects of Coptidis Rhizoma Extract(CRE) on the MN induced by cBSA in mice. Methods : Mice were divided into 4 groups. One group named for 'Normal' was injected with a saline solution not to be immunized. The rest groups were treated as follows; After mice were immunized with 0.2 mg of cBSA and Freund's complete adjuvant one time every two weeks for 6 weeks, they received intra-peritoneal injection of 10 mg/kg of cBSA daily for 4 weeks. Also, they were divided into 3 groups. The first named for 'Control' was not given CRE. The second for 'CRE-250' was given oral administration of 250 mg/kg of CRE daily for 4 weeks. The third for 'CRE-500' was given 500 mg/kg of CRE. All of mice were sacrificed 4 weeks after the first immunization. We measured a body weight and 24hrs proteinuria as well as serological analysis. The morphologic changes of renal glomeruli were also observed with a light microscope and an electron microscope. Results : The levels of 24 hrs proteinuria, triglyceride, IgG, IL-6 were significantly decreased in both CRE groups. And the level of IgM was significantly decreased in CRE-250 group. In histological findings of kidney tissue, thickening of GBM and deposition of electron-density were consideraly decreased in both CRE groups. Conclusions : The present study suggests that CRE is highly effective when treating mice with MN induced by cBSA. More clinical data and studies are to be done for efficient application.
Concentration of Heavy Metals, Residual Pesticides and Sulfur Dioxide of before/after a Decoction - In Prescription of Digestive System -
Seo, Chang-Seob ; Huang, Dae-Sun ; Lee, Jun-Kyoung ; Ha, Hye-Kyoung ; Chun, Jin-Mi ; Um, Young-Ran ; Jang, Seol ; Shin, Hyun-Kyoo ;
The Korea Journal of Herbology, volume 24, issue 1, 2009, Pages 111~119
Objectives : To compare the contents of hazardous substances before/after a decoction. Methods : The heavy metal contents before/after a decoction were measured by Inductively Coupled Plasma Atomic Emission Spectrometer (ICP-AES) and mercury analyzer. In order to analyze pesticides in 6 samples we used simultaneous multi-residue analysis of pesticides by GC/ECD, which was followed by GC/MSD analysis to confirm the identity of the detected pesticide in each sample. In addition, the contents of sulfur dioxide (SO2) were performed by Monier-Williams distillation method. Results : 1. The mean values of heavy metal contents (mg/kg) for the samples were as follows: Samchulkunbi-tang (before decoction - Pb; 1.592, Cd; 0.155, As; 0.055 and Hg; 0.014, after decoction - Pb; 0.036, Cd; 0.002, As; not detected and Hg; 0.001), Yijin-tang (before decoction - Pb; 0.830, Cd; 0.077, As; 0.045 and Hg; 0.015, after decoction - Pb; 0.193, Cd; 0.010, As; not detected and Hg; 0.002), Banhabaikchulcheunma-tang (before decoction - Pb; 0.976, Cd; 0.164, As; 0.167 and Hg; 0.019, after decoction - Pb; 0.031, Cd; 0.003, As; 0.006 and Hg; 0.005), Pyungwi-san (before decoction - Pb; 2.162, Cd; 0.128, As; 0.061 and Hg; 0.018, after decoction - Pb; 0.080, Cd; 0.006, As; not detected and Hg; 0.005), Leejung-tang (before decoction - Pb; 1.480, Cd; 0.294, As; 0.034 and Hg; 0.012, after decoction - Pb; 0.064, Cd; 0.007, As; 0.007 and Hg; 0.002) and Kwibi-tang (before decoction - Pb; 0.907, Cd; 0.193, As; 0.085 and Hg; 0.020, after decoction - Pb; 0.072, Cd; 0.006, As; 0.004 and Hg; 0.002). 2. Contents (mg/kg) of sulfur dioxide (
) before a decoction in Banhabaikchulcheunma-tang, Pyungwi-san, Leejung-tang and Kwibi-tang exhibited 3.5, 3.4, 3.8 and 12.4, respectively. However, contents of sulfur dioxide after a decoction in all samples were not detected. 3. Contents (mg/kg) of residual pesticides before/after a decoction in all samples were not detected. Conclusions : These results will be used to establish a criterion of heavy metals, residual pesticides and sulfur dioxide.
Effects of Herbal Composition and Fermented Cervi Pantotrichum Cornu on Longitudinal Bone Growth in Adolescent Male Rats
Lee, Se-Na ; Son, Jae-Bong ; Sohn, Jong-Hee ; Kim, Wan-Ki ; Lee, Sang-Jun ; Lee, Pyeong-Jae ; Leem, Kang-Hyun ;
The Korea Journal of Herbology, volume 24, issue 1, 2009, Pages 121~131
Objectives：This study was designed to investigate the effects of herbal composition and fermented Cervi Pantotrichum Cornu on the growth of longitudinal bone in the adolescent male rats. Methods： Longitudinal bone growth was measured by fluorescence microscopy. To examine the effects on the growth plate metabolism, the total height of growth plate and the induction of local insulin-like growth factor-1 (IGF-1) and bone morphogenetic protein-2 (BMP-2) were measured. Results： The herbal composition treatment enhanced longitudinal bone growth and total heght of growth plate and promoted the induction of local IGF-1 and BMP-2 of growth plate. Conclusions： This study showed that the herbal composition have the effect of bone growth in adolescent rats and might be used for the growth delayed adolescent and inherent growth failure patient.
Effects of Alcoholic Fermentation Extracts from Ovary and Rind of Pear on Human Skin
Kim, Hyung-Woo ; Kim, Kyung-Yoon ; Kim, Byung-Joo ; Chiang, Suo-Yue ; Do, Yoon-Ho ; Nam, Nai-Lee ; Jeong, Hyun-Woo ; Choi, Jeong-Sik ; Cho, Su-In ;
The Korea Journal of Herbology, volume 24, issue 1, 2009, Pages 133~139
Objectives : This study was designed to investigate the effects of alcoholic fermentation extract from ovary and rind of pear on human skin. Methods : We investigated the effects of samples on human skin in terms of clinical trial. 32 of healthy volunteers, who lived in mokpo participated in clinical trial. Effects of sample on hygroscopicity and water-holding capacity were investigated. Sebum, moisture, scabrousness and desquamation were also investigated. Facial massages were conducted 8 times in each volunteers. Results : Single treatment group showed elevated levels of hygroscopicity (Normal : 106.4
2.1%, vs Pear : 114.1
6.2) and water-holding capacity (Normal : 87.1
4.4%, vs Pear : 96.2
1.3) on forearm of 16 volunteers respectively. In addition, massage group using sample showed elevated moisture (Control : 3.8
5.8%, vs Sample : 15.2
4.7%) and lowered scabrousness levels (Control : -12.3
4.4%, vs Sample : -22.0
5.2%) significantly. However, the levels of sebum did not affected by sample. Conclusions : These result suggest that alcoholic fermentation extract from ovary and rind of pear has moisturizing effect on human skin.
Effects of Citri Reticulatae Viride Pericarpium on the Changes of Tyrosine Hydroxylase and ACTH in the Forced Swimming Test
Park, Soo-Hyun ; Lee, Tae-Hee ;
The Korea Journal of Herbology, volume 24, issue 1, 2009, Pages 141~150
Objectives : The investigation of the antidepressant effects of Citri Reticulatae Viride Pericarpium (CR) Methods : we performed the forced swimming test. Also the expression of Tyrosine Hydroxylase (TH) was measured with immunohistochemical method at the Ventral Tegmental area (VTA), Locus coeruleus (LC). The Adrenocorticotropic Hormone (ACTH) level was measured in plasma. Results: 1. The duration of immobility in the forced swimming test was significantly decreased in the CR 100 mg/kg, CR 400 mg/kg groups, comparing with the control group (p < 0.05, p < 0.001). 2. TH expressions in the VTA, LC were significantly reduced in the CR 100mg/kg and CR 400mg/kg treated group, comparing with the control group (p < 0.05, p < 0.001). 3. ACTH expression in plama was significantly reduced in the CR 100 mg/kg treated group, comparing with the control group (p < 0.05). Conclusions : According to the above results, it can be considered that Citri Reticulatae Viride Pericarpium has antidepressant effect through the reduction of TH expression at VTA, LC and ACTH level in plasma.
Effect of Water Extract from Artemisiae Argi Folium on Mouse Macrophage Stimulated by LPS
Park, Wan-Su ;
The Korea Journal of Herbology, volume 24, issue 1, 2009, Pages 151~157
Objectives : The purpose of this study is to investigate the effect of Water Extract from Artemisiae Argi Folium (WAAF) on mouse macrophage Raw 264.7 cells stimulated by lipopolysaccharide (LPS). Methods : Cell viabilities were measured by MTT assay. And the intracellular productions of hydrogen peroxide (H2O2) were measured by dihydrorhodamine 123 assay. TNF-
and IL-6 production from Raw 264.7 were measured by ELISA method. Results : The results of the experiment are as follows. 1. WAAF significantly increased the cell viability compared to the control group (treated with LPS only) at the concentrations of 10, 50, 100, 200, 400 ug/mL. 2. WAAF significantly increased the intracellular production of H2O2 compared to the control group at the concentrations of 50, 100, 200 ug/mL. 3. WAAF significantly decreased the production of TNF-
compared to the control group at the concentrations of 100, 200 ug/mL. 4. WAAF significantly decreased the production of IL-6 compared to the control group at the concentrations of 50, 100, 200 ug/mL. Conclusions : WAAF could be supposed to have the immune-modulating activity related with the macrophage's immunoactivity.
Antidiabetic Effect of So-Dang-Hwan in Streptozotocin-induced Diabetic Rats
Jung, Jin-Ki ; Park, Yong-Ki ;
The Korea Journal of Herbology, volume 24, issue 1, 2009, Pages 159~167
Objectives : So-Dang-Hwan (SDH) is used as a traditional treatment of diabetes in oriental clinics in Korea. This study aimed to evaluate antidiabetic effect of SDH in streptozotocin (STZ)-induced diabetic rats. Methods : Diabetes was induced by i.p. injection of STZ (45 mg/kg) to Sprague-Dawley rats. Experimental animals (eight per group), were treated by oral administration of SDH (60 mg/kg body weight) and glibenclimide (1 mg/kg), a known antidiabetic drug for comparison, during 5 weeks. To veridy the effect of SDH, the levels of glucose, triglyceride, insulin, BUN and creatinine were measured in sera from experimental diabetic rats, and an oral glucose tolerance test (OGTT) was also performed. Results : SDH prevented body weight loss in diabetic rats. SDH exhibited at termination, a significant reduction in blood glucose levels in STZ-induced diabetic rats. SDH significantly reduced serum creatinine levels toward the normal levels. The OGTT results showed a significant improvement in glucose tolerance in rats treated with SDH. Conclusions : These data indicate that SDH treatment may improve glocose homeostasis in STZ-induced diabetes.
The Effects of Different Extracts of Ostericum koreanum on the Production of Inflammatory Mediators in LPS-stimulated RAW264.7 Cells
Kim, Chang-Min ; Park, Yong-Ki ;
The Korea Journal of Herbology, volume 24, issue 1, 2009, Pages 169~178
Objectives : In this study, the effects of ethylacetate extract of Ostericum koreanum on inflammation in RAW264.7 cells were investigated. Methods : Dried roots of Ostericum koreanum was extracted with 80% methanol for 24 h, and then fractionated with n-butanol, n-hexan and ethylacetate. RAW264.7 cells, a mouse macrophage line were incubated with different concentrations of the extract for 30 min and then stimulated with LPS at indicated times. Cell toxicity was determined by MTT assay. The concentrations of nitric oxide (NO) and prostaglandin E2 (
) were measured by Griess assay and enzyme immunoassay (EIA), respectively. The expressions of inducible nitric oxide synthease (iNOS) and cyclooxyganase (COX) -2 mRNA and protein were determined by RT-PCR and Western blot. Results : The methanol extract of Ostericum koreanuman and its fractions were significantly inhibited the NO and PGE2 productions in LPS-stimulated RAW264.7 cells. Among the fractions of Ostericum koreanuman the ethylacetate fraction was more strongly inhibited NO and
productions compared with other fractions. The ethylacetate fraction was also suppressed LPS-induced mRNA expressions of iNOS and its protein levels in RAW264.7 cells. Conclusions : This study suggests that the ethylacetate fraction of Ostericum koreanum may have an anti-inflammatory property through suppressing inflammatory mediator productions in activated macrophages, suggesting have a therapeutic potential for the treatment of various inflammatory diseases.
Effects of Coptidis Rhizoma on the Anti-inflammation and Motor Recovery in Photothrombotic Brain Infarction Model in Rats
Lee, Su-Kyung ; Lee, In ; Shin, Sun-Ho ; Kim, Eun-Young ; Shin, Byung-Cheul ;
The Korea Journal of Herbology, volume 24, issue 1, 2009, Pages 179~189
Objectives : Coptidis Rhizoma (Coptis japonica MAKINO; CR) is a well known crude drug as antimicrobial, antibacterial, anti-inflammatory, antioxidant activity. However, there is no study of the effect of CR on brain infarction and it's mechanism. The aim of this study was to investigate the effects on ischemic stroke induced by photothrombotic infarction by evaluating the functional & neuronal recovery after brain infarction. Materials & Methods : Male Sprague-Dawley rats (250-300 g) were induced photothrombotic brain infarction on sensorimotor cortex, and brain infarction volume by image J software (NIH, USA) after Nissl stain, also single pellet reaching task as a functional motor recovery were observed. After orally pretreated by CR (500 mg/kg) or normal saline as a sham control before 7 days from the time of photothrombotic infarction, rats were sacrificed. After then we analysed anti-inflammatory cytokines (TNF-
, IL-6, IL-1
), by RT-PCR and ELISA method, and immunohistochemistry (GFAP, connexin-43) as a marker of neural plasticity. Results : CR (100, 250, 500 mg/kg) decreased the infarction volume dose-dependently, however the effect of 500mg/kg of CR (CR 500) showed the best (P=0.051). Also, CR 500 decreased the infarction volume time-dependently, the most effective time was 3-7 days after stroke. Photothrombosis increased inflammatory cytokines after infarction, CR 500 suppressed significantly mRNA expression of IL-1
, IL-6 and TNF-
. In serum, CR 500 decreased the amount of IL-1
, 12h, 24h and 48h respectively (p < 0.05), also decreased that of IL-6 and TNF-
, 12h respectively (p < 0.05) after infarction. The more astrocytes were observed and neural plasticity was facilitated in the rat brain of CR 500 than that of sham control in immunohistochemistry. Conclusions : This results suggest that CR decrease infarction volume and improve functional motor recovery in acute stage in photothrombotic ischemic infarction model in the mechanism of anti-inflammation and promoting neural plasticity.