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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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The Korea Journal of Herbology
Journal Basic Information
Journal DOI :
The Korea Association of Herbology
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Volume & Issues
Volume 24, Issue 4 - Dec 2009
Volume 24, Issue 3 - Sep 2009
Volume 24, Issue 2 - Jun 2009
Volume 24, Issue 1 - Mar 2009
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A Comparative Toxicological Study of Dried Mylabris phalerata Extract and it's Modifier : Single Dose Toxicity on Male Mice
Roh, Heui-Mok ; Kim, Seong-Mo ; Choi, Hong-Sik ;
The Korea Journal of Herbology, volume 24, issue 3, 2009, Pages 1~12
Objectives : The objective of this study was to compare, the potency of toxicity of Cantharidin containing dried Mylabis phalerata (MP) extract and it's modifier. Methods ： They were monitored at dosage level 2,000, 1,000, 500, 250 and 125 mg/kg, respectively. Changes of body weight, clinical signs, mortality, LD50, macroscopic changes of gastrointestinal tract and liver were observed after single oral dose of test articles with changes of serum Gastrin and Somatostatin levels. Results ： Dosage-dependent decrease of body weight and/or gains were demonstrated in dried MP extract-dosing groups, were also detected in modified and dried MP extract-dosing groups at 2,000 and 1,000 mg/kg-dosing group. However, below 500 mg/kg-dosing group, the body weights were significantly increased compared to that of equal dosage group of dried MP extract-dosing group. Dosage-dependently detected clinical signs in dried MP extract-dosing groups, were also detected in modified and dried MP extract-dosing groups at 2,000 and 1,000 mg/kg-dosing group. However, below 500 mg/kg-dosing group, these clinical signs dramatically were decreased compared to that of equal dosage group of dried MP extract-dosing group. Dosage-dependent increase of mortality rates were observed in dried MP extract-dosing groups, were also detected in modified and dried MP extract-dosing groups at 2,000 and 1,000 mg/kg-dosing group. However, below 500 mg/kg-dosing group, the mortalities were significantly decreased compared to that of equal dosage group of dried MP extract-dosing group. The LD50 of dried MP extract in male mice was dramaticlly increased in their modify, 265.86 vs 426.99 mg/kg. Dosage-dependently increase of number of hemorrhagic and/or erythematous spots detected in the gastrointestinal tracts of dried MP extract-dosing groups, were also detected in modified and dried MP extract-dosing groups at 2,000 and 1,000 mg/kg-dosing group. However, below 500 mg/kg-dosing group, these abnormal spots were dramatically decreased compared to that of equal dosage group of dried MP extract-dosing group. Dosage-dependently increase of degrees of enlargement and congestion detected in the liver of dried MP extract-dosing groups, were also detected in modified and dried MP extract-dosing groups at 2,000 and 1,000 mg/kg-dosing group. However, below 500 mg/kg-dosing group, these abnormal signs were dramatically decreased compared to that of equal dosage group of dried MP extract-dosing group. Dosage-dependently increase of serum gastrin levels of dried MP extract-dosing groups, were also detected in modified and dried MP extract-dosing groups at 2,000 and 1,000 mg/kg-dosing group. However, below 500 mg/kg-dosing group, these abnormal increase were dramatically decreased compared to that of equal dosage group of dried MP extract-dosing group. Dosage-dependently increase of serum somatostatin levels of dried MP extract-dosing groups, were also detected in modified and dried MP extract-dosing groups at 2,000 and 1,000 mg/kg-dosing group. However, below 500 mg/kg-dosing group, these abnormal increase were dramatically decreased compared to that of equal dosage group of dried MP extract-dosing group. Conclusions ： The toxicity of dried MP extract was reduced by their modify.
Protective Effect of the Water Extract of Tissue Cultured Root of Wild Ginseng Against Doxorubicin Toxicity
Choi, Kyung-Un ; Lee, Pyeong-Jae ; Kim, Ho-Hyun ;
The Korea Journal of Herbology, volume 24, issue 3, 2009, Pages 13~19
Objectives : This study was aimed to define the protective effect of Tissue Cultured Root of Wild Ginseng (CWG) against doxorubicin (Doxo) toxicity, and investigate the anti-tumor synergic effect of CWG in combination with Doxo in tumor-bearing C57BL/6 mice. Methods : Tumor-bearing mice were established by single inoculation with B16/F10 melanoma cells (2
/ml) subcutaneously. Tumor-bearing mice (tumor volume between 50-100 mm
) were selected and divided them into control, Doxo, and Doxo+CWG group. Mice of Doxo group were received with Doxo (4 mg/kg of B.W.) intraperitoneally at 0, 4, 8 days after starting the experiment. Mice of Doxo+CWG group were received CWG water extract during 12 days in combination with Doxo treatment. The body weight, tumor volume, tumor weight, and organ weight (heart, liver, kidney, and testis) were measured. And serum SPK, GOT and creatinine values were analysed. Results : The volume and weights of tumor masses in Doxo group were decreased significantly compared with the those of control group. And the those of Doxo+CWG group were not significantly different from the those of Doxo group. Whereas the weight of body, liver, kidney and testis in Doxo+CWG group were increased significantly compared with the those of Doxo group. The level of serum CPK and GOT in Doxo group were increased compared with the those of control group. But the value of Doxo+CWG group were decreased significantly compared with the values of Doxo group. Conclusions : These results suggest that CWG has protective effect against doxorubicin toxicity. And these effect is guessed that is caused in augmentation of vital energy.
A Study on the Herbal Name and Four Qi(四性) of Genus Curcuma in the Korea Pharmacopoeia Ninth Edition
Kim, In-Rak ;
The Korea Journal of Herbology, volume 24, issue 3, 2009, Pages 21~28
Objectives : The purpose of this study is to determine the herbal name and four Qi(四性) of genus curcuma in the Korea Pharmacopoeia ninth Edition. Methods : Compare with the name and four qi of genus curcuma from the Korea Pharmacopoeia ninth Edition and the other nation's Pharmacopoeia and main herbal medicine books. Results : 1. The Rhizoma of Curcuma longa is called Kanghwang in KP 9, but is called Ulgeum in the main herbal medicine book. 2. The Rhizoma of genus curcuma except Curcuma longa is called Achul in KP 9, but is called Kanghwang in the main herbal medicine book. 3. The root of genus curcuma is called Ulgeum in KP 9, but is called Achul in the main herbal medicine book. 4. The four qi of the Rhizoma and Radix of genus curcuma is recorded worm and cold in the Ch. p 2005, but is recorded cold and worm in the main herbal medicine book. Conclusions : Kanghwang, Achul, Ulgeum in the KP 9 is equivalent Ulgeum, Kanghwang, Achul in the main herbal medicine book. The four qi of Rhizoma is cold, Radix is warm.
Inhibitory Effects of Trichosanthis Radix in the Activity and Proliferation of Th2 T Cells and Eosinophils in vitro : Implications on its Regulatory Roles for Asthma
Roh, Seong-Soo ;
The Korea Journal of Herbology, volume 24, issue 3, 2009, Pages 29~37
Objectives : The present study was carried out to investigate the effect of Trichosanthis Radix extract (TRE) on the proliferation and activation of eosinophils which were prepared from lung cells of asthma-induced mice by ovalbumin (OVA) treatment. Methods : C57BL/6 mouse was exposed to OVA three times a week for 6 weeks. The mouse lung tissues were dissected out, chopped and dossiciated with collagenase (1
g/ml). Eosinophils were activated by rmIL-3/rmIL-5 co-treatments. The lung cells were treated with TRE, incubated for 48 hr at 37
, and analyzed by flow cytometer, ELISA and RT-PCR methods Results : To measure cytotoxicity, mouse lung fibroblast cells (mLFCs) were pretreated with various concentrations of TRE. TRE at 100
g/ml, the highest concentration, examined did not have any cytotoxic effects on mLFCs. In FACS analysis, number of granulocyte/lymphocyte, CD3e-/CCR3+, CD3e+/CD69+, CD4+/CD8+ T cells in asthma-induced lung cells were significantly decreased by TRE treatment compared to the control group. But CD4+/CD25+ T cells were not examined significant change in lung cells treated with TRE. In ELISA analysis, production levels of IL-3, IL-5, IL-13 and histamine in asthma-induced lung cells, which were induced by rIL-3 plus rmIL-5 co-treatment, were significantly decreased by TRE treatment. Conclusions : The present data suggested that Trichosanthis Radix on the inhibition of parameters associated with asthma responses in eosinpophils, and thus implicate the possibility for the clinical application of Trichosanthis Radix.
Anti-oxidative and Anti-inflammatory Effect of Fractionated Extracts of Smilacis Glabrae Rhizoma in Human Umbilical Vein Endothelial Cell
Lee, Chang-Hyun ; Yi, Hyo-Seung ; Kim, Jae-Eun ; Heo, Sook-Kyoung ; Cha, Chang-Min ; Won, Chan-Wook ; Park, Sun-Dong ;
The Korea Journal of Herbology, volume 24, issue 3, 2009, Pages 39~50
Objectives : Smilacis glabrae rhizoma (SG) has been traditionally used as a herbal medication of musculoskeletal disorders like arthritis, pain, convulsions, and syphilis in traditional Korean medicine. This study was investigated anti-oxidative and anti-inflammatory effect of fractionated extracts of Smilacis Glabrae Rhizoma in Human Umbilical Vein Endothelial Cell (HUVEC). Methods : SG extract prepared with methanol, and then fractionated with hexane, dichloromethane, ethylacetate, n-butanol and water. Inhibitory effect of SG onto free radical generation was determined by measuring DPPH, superoxide anions and nitric oxide scavenging activities in vitro. Cytotoxic activity of extracts on RAW 264.7 cells was measured using 5-(3-caroboxymethoxyphenyl)-2H-tetra-zolium inner salt (MTS) assay. Intracelluar oxidation was analysed by DCF-DA assay. The nitric oxide (NO) production was measured by Griess reagent system. The levels of ICAM-1 and VCAM-1 expression were confirmed by western blot. And proinflammatory cytokines were measured by ELISA kit. Results : Our results indicated that fractionated extracts, especially ethyl acetate (EA) extract, significantly inhibited free radical generation, the TNF-
-induced intracellular oxidation. Furthermore, the EA extract protected TNF-
-induced adhesion to THP-1, expression of adhesion molecules accompanied by an attenuation of IL-6 and IL-8 formation in HUVEC. Conclusions : These results indicate that EA extract of SG have potential as an agent of atherosclerosis and other chronic inflammatory diseases including diabetes, hypertension, and arthritis.
Changes in Biological Activities of Extracts from Herbal Chokong Pills by Manufacturing Stages
Kim, Dong-Han ; Park, Chan-Sung ;
The Korea Journal of Herbology, volume 24, issue 3, 2009, Pages 51~58
Objectives : This study was conducted to evaluate the antioxidative and anticancer activity of the water and ethanol extracts from pickled soybean products by manufacturing stage. Methods : Yakkong (YK) was pickled for 15 days to prepare Chokong (CK) and Chokong pills (CKP) were mixed Chokong (CK) powder with vinegar. Herbal Chokong pills (HCKP) were made by addition five kinds of medicinal herbs to Chokong. Pickled soybean products by each manufacturing process were extracted with distilled water and 70% ethanol, and the extracts were tested for their antioxidative and anticancer activities. Results : The highest electron donating abilities (EDA) of the water and ethanol extracts were from HCKP among pickled soybean products and those were 81.2% and 91.5% at 1,000
g/mL, respectively. The ethanol extracts of pickled soybean products had higher activities of EDA and superoxide dismutase (SOD) than water extracts. The highest anticancer activity was the water extracts of HCKP against both of MDA and A549 cells. Conclusions : These results suggest that herbal Chokong pills (HCKP) can be used as natural antioxidant to prevent oxidative damage in normal cells probably because of their antioxidative characteristics.
Molecular Authentication and Phylogenetic Relationship of Bupleurum Species by the rDNA-ITS Sequences
Moon, Byeong-Cheol ; Choo, Byeong-Kil ; Ji, Yun-I ; Yoon, Tae-Sook ; Lee, A-Young ; Cheon, Myeong-Sook ; Kim, Bo-Bae ; Kim, Ho-Kyoung ;
The Korea Journal of Herbology, volume 24, issue 3, 2009, Pages 59~68
Objectives : Bupleuri Radix (Siho) is prescribed as the root of different Bupleurum species on the pharmarcopoeia in Korea and China. Moreover, other species and varieties of the genus Bupleurum have been also distributed on the herbal market as Bupleuri Radix. However, due to the morphological similarity and frequent occurrence of intermediate forms, the correct identification of this radix is very difficult. To develop a reliable method for correct identification and improving the quality standards of official Bupleuri Radix, we analyzed sequences of the ribosomal RNA gene and internal transcribed spacer (rDNA-ITS) region. Methods : PCR amplification of rDNA-ITS region was performed using ITS1 and ITS4 primer from 6 Bupleurum species and 1 variety, B. falcatum L. (Siho), an improved breed of B. falcatum L. (Samdo-Siho), B. chinense DC. (Buk-Siho), B. scorzonerifolium Willd. (Nam-Siho), B. longiadiatum Turcz. (Gae-Siho), B. euphorbiodes Nakai (Deungdae-Siho) and B. latissimum Nakai (Seom-Siho), and nucleotide sequence was determined after sub-cloning into the pGEM-Teasy vector. Authentic marker nucleotides were estimated by the analysis of ClastalW using entire rDNA-ITS sequence of three samples per species. Results : In comparative analysis of the rDNA-ITS sequences, we found specific nucleotides to distinguish Korean (B. falcatum L. and its variety) and Chinese official species (B. chinense DC. and B. scorzonerifolium Willd.) from others at positions 411 and 447, and positions 89, 101, 415 and 599, respectively. Futhermore, we also found nucleotide indels (insertion and/or deletion) and substitutions to identify each of different Bupleurum species, 2 positions for B. falcatum L. and its variety, 6 positions for B. chinense DC., 49 positions for B. scorzonerifolium Willd., 8 positions for B. euphorbioides Nakai, 7 positions for B. longiradiatum Nakai and 9 positions for B. latissimum Nakai. These sequence differences at corresponding positions are avaliable nucleotide markers to determine the botanical origins of Bupleuri Radix. Moreover, we confirmed the phylogenetic relationship of B. latissimum Nakai, a Korean endemic speices, among Bupleurum species based on the rDNA-ITS sequence. Conclusions : These marker nucleotides would be useful to identify the official herbal medicines by the providing of definitive information that can identify each plant species and distinguish it from unauthentic adulterant Bupleurum species.
Effects of Horse Bone Powder Extract on Longitudinal Bone Growth in Adolescent Male Rats
Lee, Se-Na ; Choi, Han ; Son, Jae-Bong ; Jeong, Jae-Seung ; Kim, Yun-Jung ; Kang, Dae-Pyung ; Park, Soo-Yeong ; Kang, Se-Chan ; Leem, Kang-Hyun ;
The Korea Journal of Herbology, volume 24, issue 3, 2009, Pages 69~77
Objectives : This study was designed to investigate the effects of horse bone powder extract on the growth of longitudinal bone in adolescent male rats. Methods： Longitudinal bone growth was measured by fluorescent microscopy. To examine the effects on the growth plate metabolism, the total height of growth plate, the induction of local insulin-like growth factor-1 (IGF-1), and bone morphogenetic protein-2 (BMP-2) were measured. Results： Horse bone powder extract enhanced longitudinal bone growth and total height of the growth plate. Also, it promoted the induction of local IGF-1 and BMP-2 of the growth plate. Conclusions： This study shows that the horse bone powder extract effects longitudinal bone growth in adolescent rats and might be used for both stunted adolescents and inherent growth failure patients.
Anti-inflammatory Effect of Coptidis Rhizoma
Yoon, Kwang-Ro ; Kim, Young-Jin ; Lee, Eun ; Lee, Joon-Moo ;
The Korea Journal of Herbology, volume 24, issue 3, 2009, Pages 79~86
Objectives : The present study investigated Inflammatory effect of Coptidis Rhizoma in lipopolysaccharideexposed rats and Raw 264.7 cells. Methods： The plasma concentration of IL-1
, IL-6 and TNF-
peaked at 5 h after LPS injection, and the values of the Coptidis Rhizoma extract groups were lower than those of the control group. In the increment of cytokines concentration at 2 h and 5 h after LPS injection, the Coptidis Rhizoma groups were lower than that of control group. The plasma concentration of IL-10 peaked at 5 h after LPS injection, and the values of the Coptidis Rhizoma extract groups were higher than those of the control group. In the increment of cytokines concentration at 2 h and 5 h after LPS injection, the Coptidis Rhizoma groups were higher than that of control group. Liver cytokines measurement was done at 5 h after LPS injection. The concentration of liver IL-1
and IL-6 in the Coptidis Rhizoma groups was lower than that of the control group. The concentrations of liver TNF-
, and IL-10 showed no significant differences among all the treatment groups. Results： In the studies of lipopolysaccharide-exposed Raw 264.7 cells, the concentration of IL-1
, IL-6 and TNF-
in the lipopolysaccharide-exposed cells groups was higher than that of control group (normal group), and in the lipopolysaccharide-exposed cells groups, these values showed a tendency to decrease in the Coptidis Rhizoma groups. The concentration of IL-10 in the lipopolysaccharide-exposed cells groups was higher than that of control group (normal group), and in the lipopolysaccharide-exposed cells groups, the values showed a tendency to increase in the Coptidis Rhizoma groups. Conclusions： These results indicate that the Coptidis Rhizoma extracts have an functional material for Inflammatory activities.
Effects of Extruded Acanthopanax Folium Extracts on Complete Freund's Adjuvant Induced Arthritis of Rats
Lee, Se-Na ; Seo, Il-Bok ; Son, Jae-Bong ; Kim, Hye-Kyung ; Leem, Kang-Hyun ;
The Korea Journal of Herbology, volume 24, issue 3, 2009, Pages 87~96
Objectives : This study was designed to investigate the effects of extruded Acanthopanax Folium extracts on Complete Freund's Adjuvant (CFA) induced arthritis of rats. Methods： To induce arthritis in the ankle joint of rats, CFA was injected in the proximal part of the tail subcutaneously. After CFA injection, arthritic conditions were examined with macrography. The volume of paw edema and thickness of the ankle joints were checked regularly within 20 days. At 20 days, histopathological examination was performed on the ankle joint. Inflammation levels were determined by total WBC counts and differential WBC counts using a blood analyzer. Tumor necrosis factor-
concentration in paw exudate were measured by ELISA method. Results： Several arthritic conditions induced by CFA were alleviated by Acanthopanax Folium treatment. Morphologically, reduction of arthritic conditions were observed and the volume of paw edema and thickness of the ankle joints were significantly decreased. Additionally, cytokines in paw exudate were diminished and histopathological improvement was observed. Conclusions： This study showed that the extruded Acanthopanax Folium extracts have the beneficial effects on the CFA induced arthritis of rats and might be used for chronic arthritis patients.
Anti-inflammatory Effects of Herbal Formula KCNS-001 for Mitigating Atopic Dermatitis
Lee, Jeong-Bok ; Choi, Jae-Hwan ; Bang, Ok-Sun ; Yu, Young-Beob ;
The Korea Journal of Herbology, volume 24, issue 3, 2009, Pages 97~102
Objectives : We determined the anti-inflammatory activity of KCNS-001 that is a herbal formula including 6 medicinal plants and that are used to mitigate atopic dermatitis in oriental medicine. Methods : To evaluate anti-inflammatory effect of KCNS-001, we measured the production of reactive oxygen species (ROS), nitric oxide (NO) and cyclooxygenase-2 (COX-2) in LPS-activated Raw 264.7 cells. Cell viability was determined by MTT assay. The concentrations of ROS and relative level of NO were measured with DPPH assay and Griess reagent, respectively. COX-2 and TNF-
were detected by enzyme immuno assay (EIA) and enzyme-linked immunosorbent assay (ELISA). Results : ROS and NO production were reduced by KCNS-001 in a dose-dependent manner. KCNS-001 significantly inhibited activity of COX-2 and suppressed the release of tumor necrosis factor-alpha (TNF-
). Conclusions : These results indicate that the KCNS-001 may have an anti-inflammatory agent for the treatment of various inflammatory disease.
Anti-oxidative Effects of Taraxaci Herba and Protective Effects on Human HaCaT Keratinocyte
Kim, Hyung-Woo ; Kim, Byung-Joo ; Lim, Se-Hyun ; Kim, Hyun-Yung ; Lee, Sook-Young ; Cho, Su-In ; Kim, Young-Kyun ;
The Korea Journal of Herbology, volume 24, issue 3, 2009, Pages 103~108
Objectives : This study was carried out to investigate anti-oxidative effects of Taraxaci Herba (TH) and protective effects on Human HaCaT keratinocyte. Methods : Anti-oxidative effects were measured by estimating the amount of total phenolics and flavonoids. In addition, DPPH free radical scavenging activities were estimated. Protective effects of TH on HaCaT keratinocytes against oxidative stress induced by hydrogen peroxide were also measured. Results : In our results, treatment with TH did not show cytotoxicity on HaCaT keratinocyte beneath the concentration of 200
g/ml of total phenolics and 28.09
g/ml of flavonoids was detected from TH ethanol extract. In addition, DPPH free radical scavenging activities of TH were elevated in dose-dependent manner. In addition, The value of half maximal inhibitory concentration (IC
) was 165.5
g/ml. Finally, TH showed protective effect against cell death of HaCaT cell induced by hydrogen peroxide significantly. Conclusions : In conclusion, these results suggest that TH may have anti-oxidantic action in human skin and also suggest the possibility as cosmetic material.
Study on the Anti-oxidative Effects of Adenophorae Radix, Codonopsis lanceolatae Radix and Glehniae Radix Cum Rhizoma on Liver Cells Isolated from Oxidatively Stressed Rat
Min, Sang-Hong ; Han, Hyo-Sang ; Lee, Young-Jong ;
The Korea Journal of Herbology, volume 24, issue 3, 2009, Pages 109~119
Objectives : The present study was purposed to compare Adenophorae Radix (henceforth AR), Codonopsis lanceolatae Radix (henceforth ClR) and Glehniae Radix cum Rhizoma (henceforth GRcR) concerning their anti-oxidant effect. Methods : We measured eythrocyte, leukocyte, thrombocyte, serum albumin, total bilirubin, LDL cholesterol, and glucose as well as SOD, GSH, catalase, NO, and MDA in the rat liver oxidatively stressed by AAPH. Results : 1. The oxidative stress-induced thrombocyte levels were significantly decreased in CIR-treated and GRcR-treated groups. 2. The oxidative stress-impaired SOD acitivities were significantly recovered in AR-treated and GRcR-treated groups. 3. The oxidative stress-reduced GSH contents were significantly increased in ClR-treated and GRcR-treated groups. 4. The oxidative stress-reduced catalase contents were significantly increased in all of the three groups. 5. The oxidative stress-induced NO productions were significantly decreased in all of the three groups. Conclusions : AR, ClR, and GRcR altogether showed the anti-oxidant effect in the rat liver oxidatively stressed by AAPH. The anti-oxidant properties of tAR, ClR, and GRcR seem to be similar even if those have different botanical properties and different medical efficacies in oriental medicine.
The Inhibitory Effect of Cudraniae Lignum on the Collagen-induced Arthritis in Mice
Choi, Jeong-Ho ; Han, Hyo-Sang ; Lee, Young-Jong ;
The Korea Journal of Herbology, volume 24, issue 3, 2009, Pages 121~128
Objectives : The present study purposed to examine the effect of Cudraniae Lignum, which is the wooden part of Cudrania tricuspidata Bureau (Moraceae), on cytokine secretion from the joint cells of mice with arthritis induced by collagen and verify its efficacy against rheumatoid arthritis. Methods : We prepared the hot-water extract, ethanol extract and methanol extract of Cudraniae Lignum, and tested their effects on cytokine secretion from the joint cells of mice with arthritis induced by collagen. Results : 1. Cudraniae Lignum reduced the secretion of inflammatory cytokines such as IL-1
and IL-6 from joint cells. 2. The effect for reducing the secretion of IL-1
and IL-6 was strong in order of methanol extract, ethanol extract and hot water extract. 3. Considering the effect on cytokine secretion, the effective element is soluble in organic solvent, and is not volatile. 4. The ethanol extract and methanol extract of Cudraniae Lignum lowered the survival rate of cells significantly, but still the survival rate was over 92%, suggesting the low toxicity of the extracts. Conclusions : Cudraniae Lignum is considered effective for rheumatoid arthritis, and the effective element is considered soluble in organic solvent.
Study on Antioxidant Effect of Hoveniae Semen cum Fructus and Hoveniae Ramulus on Liver Cells Isolated from Oxidatively Stressed Rat
Choi, Jeong-Kook ; Han, Hyo-Sang ; Lee, Young-Jong ;
The Korea Journal of Herbology, volume 24, issue 3, 2009, Pages 129~138
Objectives : The Objective of this study is to compare the anti-oxidative effect of the Hoveniae Semen cum Fructus (HScF) to that of Hoveniae Ramulus (HR) in vivo. Methods : The extracts of HScF and HR were tested in vivo for their anti-oxidative effects. SD Rats were injected with the AAPH (2.2'-azobis (2-aminodinopropane) hydrochloride) to induce the acute oxidization stress, and the oxidized rats were administered with the HScF and HR decoction orally. The RBC, WBC and PLT populations in plasma, and the blood chemistry levels of the rats were measured to observe the overall changes in the blood. The anti-oxidative effects of the HScF and HR decoctions were investigated on the liver tissues of the oxidated rats. Results : 1. Thrombocyte decreased significantly in the group medicated with HScF. 2. Blood glucose increased significantly in the group medicated with HR. 3. SOD activity increased significantly in both the group medicated with HScF and that with HR. 4. NO concentration decreased significantly in both the group medicated with HScF and that with HR. 5. The catalase content increased significantly in both the group medicated with HScF and that with HR. Conclusions : The HScF and HR extracts have an anti-oxidative effect.
Effect of HTE001, an Herbal Formulation, on Electric Stimulation-induced Penile Erection in Rats
Lim, Dong-Wook ; Lee, Dong-Hun ; Song, Mi-Kyoung ; Kim, Mi-Yeon ; Bu, Young-Min ; Kim, Ho-Cheol ;
The Korea Journal of Herbology, volume 24, issue 3, 2009, Pages 139~146
Objectives : This study was conducted to investigate the effect of HTE001, a multi-herbal mixture consisting of 10 herbs, Cornus Frutus, Schizandrae Fructus, Rubi Fructus, Cnidi Fructus, Acanthopanacis senticosi Radix, Cinnamomi Cortex, Eucommiae Cortex, Allii Bulbus, Rehmanniae Radix and Ginseng Radix, on electrostimulation-induced penile erection in rats. Methods : Intracavernous pressure (ICP) and mean arterial blood pressure (MAP) were simultaneously monitored through electric stimulation of the cavernous nerve after the oral administration of HTE001 (30, 100, 300 mg/kg) in normal rats. Statistical analysis was performed on maximal intracavernous pressure (ICP), maximal intracavernous pressure/mean arterial blood pressure (ICP/MAP) ratio, and the area under the curve (AUC) of ICP/MAP ratio. Results : Oral administration of HTE001 300 mg/kg caused the ICP to increase in a frequency-dependent manner. And HTE001 300 mg/kg treatment group showed the highest value in the ICP/MAP ratio and the AUC value of the ICP/MAP ratio compared to the control group at 2 Hz, 6 Hz and 10 Hz, respectively without an effect on the mean arterial blood pressure under the same stimulation of the cavernous nerve. Conclusions : These results show that HTE001 improve penile erection and prolong the decay period in normal rats without affecting mean arterial blood pressure, and suggest that HTE001 could be a good therapeutic candidate to treat erectile dysfunction.
Bibliographic Study of Jejungsinpyeon Yakseongga
Lee, Jeong-Hwa ; Ahn, Sang-Woo ;
The Korea Journal of Herbology, volume 24, issue 3, 2009, Pages 147~152
Objectives : This paper intends to shed light on the value of Jejungsinpyeon, which is known to have had a considerable impact on the country's medicine in the later period Joseon Period, and its position in the Joseon history of medicine. To such an end, this paper focuses on how Yakseongga was developed and transformed in the later period Joseon Period through a bibliographical study of it. Methods : (1) To make comparison and analysis of the 303 lines, which the author said he quoted from Wan.bing.hui.chun and Susebowon, with Yakseongga of Jejungsinpyeon to see association between them based on Yakseongga contained in Jejungsinpyeon (2) To make a table concerning the names of the books, their authors, when they were published, how many kinds there are, their formats based on a survey of literature containing Yakseongga published after Jejungsinpyeon. (3) To see influences made by Jejungsinpyeon on books of medicine in the later Joseon Period. Results : It was found that a total of 303 lines had been quoted from Wan bing hui chun and Susebowon and that 83 lines had been newly inserted by the author. As a result of an analysis of Yakseongga of Jejungsinpyeon, Of the 303 lines, 297 were quoted from Susebowon, and only six from Wan bing hui chun. This means that the author of Jejungsinpyeon chiefly used Susebowon as the source of Yakseongga quoted therein. 275 lines, or 76%, of a total of 386 lines of Yakseongga of Jejungsinpyeon, have their Korean names marked. Medicinal herbs marked as Chinese herbs number 83 out of 386. Among the 83 herbs named, one belongs to the part newly inserted by the author, while the remaining 82 are quoted from Yakseongga. Conclusions : It shows the author's intention to make what is contained in Yakseongga localized knowledge and used by the people easily, although Yakseongga was compiled based on quotations from Chinese literature.
Inhibitory Effect of Extract of Trogopterorum Faeces on the Production of Inflammatory Mediaters
Kim, Byung-Jin ; Ham, Kyung-Wan ; Park, Kyung-Bae ; Kim, Dae-Hyeon ; Jo, Beom-Yeon ; Cho, Chang-Re ; Cho, Gil-Hwan ; Bae, Gi-Sang ; Park, Kyoung-Chel ; Koo, Bon-Soon ; Kim, Min-Sun ; Song, Ho-Joon ; Park, Sung-Joo ;
The Korea Journal of Herbology, volume 24, issue 3, 2009, Pages 153~160
Objectives : The purpose of this study was to investigate the anti-inflammatory effects of extract from Trogopterorum Faeces (TF) on the RAW 264.7 cells. Methods : To prove the TF's anti-inflammatory effects, we investigated nitric oxide (NO) production and own cell viability. We examined the cytokine productions on lipopolysacchride (LPS)-induced RAW 264.7 cells and also cellular regulatory mechanisms. Results : TF does not have any cytotoxic effect. TF reduced LPS-induced NO production, interleukin (IL)-1b, IL-6, IL-10 and tumor necrosis factor-a (TNF-a) in RAW 264.7 cells. TF inhibited the activation of mitogen-activated protein kinases (MAPKs) such as p38, extracelluar signal-regulated kinase (ERK 1/2) and c-Jun NH2-terminal kinase (JNK) and also the degradation of inhibitory kappa B a (Ik-Ba) in the LPS-stimulated RAW 264.7 cells. TF reduced the serum levels of IL-1b, IL-6, TNF-a. The survival rate of LPS-induced endotoxin shock was increased by TF administration. Conclusions : TF down-regulated LPS-induced NO and cytokines production, which could provide a clinical basis for anti-inflammatory properties.
Anti-carcinogenetic and Anti-metastatic Effects of Extract from Maekmoondong-tang in HepG2 Cells
Cheon, Myeong-Sook ; Chun, Jin-Mi ; Yoon, Tae-Sook ; Lee, A-Yeong ; Moon, Byeong-Cheol ; Choo, Byung-Kil ; Kim, Seong-Hwan ; Kim, Ho-Kyoung ;
The Korea Journal of Herbology, volume 24, issue 3, 2009, Pages 161~167
Objectives : Maekmoondong-tang (MMDT), a Korean herbal medicine, has been used to treat severe dry cough in patients with bronchitis and pharyngitis. MMDT has been reported to have anti-inflammatory, anti-allergic, immunomodulatory, secretory-modulating, and metabolic regulatory actions. However, there are no evidence in regard to the effects of MMDT on carcinogenesis and metastasis. Here, we investigated the effects of 70% ethanol extract of MMDT on cell viability, apoptosis, and motility in human hepatocarcinoma HepG2 cells. Methods : Cell viability was measured using the CCK-8 assay, and the apoptosis induction was evaluated by caspase-3 activity. To detect apoptotic features, the cells treated with MMDT were stained with 4'-6-diamidino-2-phenylindole (DAPI). Cell motility was examined by Boyden chamber assay and Real-time Cell Index of Migration assay. Gelatin zymography also performed to measure matrix metalloproteinase (MMP)-2/9 activity. Results : We found that MMDT significantly inhibited cell proliferation and increased caspase-3 activity in a dose-dependent manner in HepG2 cells. Apoptotic features such as chromatin condensation and apoptotic bodies were observed in MMDT-treated cells by DAPI staining. MMDT also suppressed PMA-induced cell motility and activities of MMP-2/9. Conclusions : Our results exhibited that MMDT possess the anti-carcinogenetic and anti-metastatic activities via caspase-3 activation and down-regulation of cell motility and invasion in HepG2 cells. Therefore, these findings suggest that MMDT could be potentially applied to the prevention and treatment of cancer.
Discussion of Ginseng Properties through a Historical Research of Korean Ginseng
Ko, Sung-Kwon ; Leem, Kang-Hyun ;
The Korea Journal of Herbology, volume 24, issue 3, 2009, Pages 169~172
Objectives : The purpose of this study is to discuss ginseng properties by historical research. Methods : Historical documentary records of ginseng were used to develop this review. Results : According to the historical research, the first Korean ginseng cultivation started from the Koryo Dynasty around A.D. 1000. Later, during the Chosun Dynasty around A.D. 1500
1600, the ginseng cultivators established a method of cultivation. Gasam (cultivated ginseng) was cultivated ginseng on the skirts of their domicile. It was reported that Gasam cultivation was broadened throughout the country around A.D. 1790 on Cheongjosilrok. On the other hand, ginseng properties were reported slightly cool in the oriental herbal medicine books from A.D. 250 to A.D. 1600. But, they were reported slightly warm or warm after A.D. 1600. Korean ginseng cultivation started in the Koryo Dynasty around A.D. 1000. Later, during the Chosun Dynasty, the ginseng cultivators established a standard method of cultivation. Gasam, cultivated ginseng, was grown on the outskirts of their domicile. It was reported that Gasam cultivation was broadened throughout the country around 1790 A.D. in Cheongjosilrok. On the other hand, ginseng properties were reported slightly cool in the oriental herbal medicine books from 250 to A.D. 1600, but they were reported slightly warm or warm after A.D. 1600. Conclusions : It suggests that ginseng properties (slightly cool) before A.D. 1600 are wild ginseng. Also, wood-grown ginseng and ginseng properties (slightly warm, warm) after A.D. 1600 are Gasams.