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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
The Korea Journal of Herbology
Journal Basic Information
Journal DOI :
The Korea Association of Herbology
Editor in Chief :
Volume & Issues
Volume 25, Issue 4 - Dec 2010
Volume 25, Issue 3 - Sep 2010
Volume 25, Issue 2 - Jun 2010
Volume 25, Issue 1 - Mar 2010
Selecting the target year
A Chromosome Aberration Test of HMC05 on Cultured Chinese Hamster Lung Cells
Shin, Heung-Mook ;
The Korea Journal of Herbology, volume 25, issue 1, 2010, Pages 1~7
Objectives : We investigated genetic toxicity of HMCO5 in relation to chromosome aberration test on Cultured Chinese Hamster Lung (CHL) in the presence and absence of S-9 mix. Methods : Experimental groups were divided into two groups: with S-9mix (+S) or without S-9 mix (-S). -S group was also divided 2 series by treatment hours (6 hr: 6-S; or 24 hr; 24-S). Each group treated with vehicle only (complete culture medium), HMCO5 (1,250, 2,500,
), and cyclophosphamide monohydrate (CPA) and ethylmethanesulfonate (EMS), respectively. Results : HMC05 did not show any aberrant metaphase. However, there were significant (p < 0.01) aberrant metaphases with CPA in S+ and with EMS in S-. Conclusions : These results indicate that HMC05 formula does not show any toxicity in chromosome aberration test.
Comparative Study of Extraction Efficiency of Water Decoction with or Without Non-woven Fabric
Yang, Woong-Mo ; Chung, Kyu-Jin ; Kim, Kyung-Wook ; Bae, Hyun-Su ; Chang, Mun-Seog ; Park, Seong-Kyu ;
The Korea Journal of Herbology, volume 25, issue 1, 2010, Pages 9~12
Objectives : To evaluate the extraction efficiency of water decoction with or without non-woven fabric underlying comparison of the HPLC (high-performance liquid chromatography) profiles of the hesperidin which is the standard of Citri Pericarpium. Methods : Pyungwisan, which is comprised of Atractylodis Rhizoma, Magnoliae Cortex, Citri Pericarpium, Glycyrrhizae Radix, Zingiberis Rhizoma, and Ziziphy Fructus, was extracted in boiling water with non-woven fabric or without non-woven fabric. Then hesperidin content was analyzed by HPLC. Results : The contents of hesperidin in the water extract of Pyungwisan which was decocted without non-woven fabric showed
in with non-woven fabric decoction. Conclusions : These results suggest that herbal decoction without non-woven fabric might increase extraction efficiency.
The Study of the Anti-oxidative Effects of Polygonati Multiflori Caulis on Oxidized Brain and Liver Cells in Rats
Lee, Han-Goo ; Han, Hyo-Sang ; Lee, Young-Jong ;
The Korea Journal of Herbology, volume 25, issue 1, 2010, Pages 13~21
Objectives : This study was purposed to the anti-oxidative effects of Polygonati Multiflori Caulis(henceforth PMC) on oxidized brain and liver cells in rats. Methods : After extraction of PMC with water, the water extract was divided into five fractions : hexane, ethyl ether, ethyl acetate, butanol and an aqueous fraction. The phenol contents of each fraction were measured. The lipid peroxidation inhibition effect were then investigated. Results : After processing PCM water and PCM fractionations on oxidized brain cells in rats, the SOD (super oxide dismutase) activity and glutathione content were increased, and the NO (nitric oxide) content was decreased. It had much higher SOD activity than liver cells in rats excluded in the n-BuOH and aqueous fractions. In case of oxidized liver cells in rats, the SOD activity and glutathione content increased, while both the NO content and the MDA (malondialdehyde) content decreased. It had much higher glutathione content than brain cells in rats in the every fractions. It had much lower MDA content than brain cells in rats in the Aqueous fractions and brain cells in rats had much lower MDA content than liver cells in rats in the total extract, n-hexane, EtOEt, EtOAc and n-BuOH fractions. Conclusions : PMC has anti-oxidative effect on oxidized liver cells and brain cells in rats, through there are differences in fraction. Additionally, Anti-oxidative effect of brain cells can be relaxed the mental nerve and it is related PMC effect.
A Comparative Study of the Anti-cancer Effects of Smilacis Chinae Rhizoma and Smilacis Glabrae Rhizoma on Stomach Cancer SNU-1 Cells
Ahn, Han-Kyu ; Han, Hyo-Sang ; Lee, Young-Jong ;
The Korea Journal of Herbology, volume 25, issue 1, 2010, Pages 23~31
Objectives : The purpose of this study was to compare the anticancer effects of Smilacis Chinae Rhizoma (SCR) and Smilacis Glabrae Rhizoma (SGR) on stomach cancer SNU-1 cells. Methods : The cell proliferation, apoptosis and cytokine level from the extracts were examined in order to compare the anticancer effects of SCR and SGR on SNU-1 cells. Results : SCR had greater anticancer effects than SGR in terms of the inhibition of cell proliferation and apoptosis in the SNU-1 cells. SCR decreased TGF-
and SGR increased IL-2 in SNU-1 cells. SCR decreased more TGF-
and increased more TNF-
compared to SGR. Conclusions : There were few significant differences according to the concentration and fraction, but a greater anticancer effect of SCR was shown as compared with SGR.
Effects of NR on Production Levels of Cytokines and Distribution of BAL Cells in Allergic Asthma
Bae, Jin-Hyun ; Yoo, Ji-Hyun ; Kil, Gi-Jung ;
The Korea Journal of Herbology, volume 25, issue 1, 2010, Pages 33~38
Objectives : This study was designed to investigate therapeutic effects fo NR on allergic asthma in terms of measurement of cytokine profiles, distribution of BAL and expression levels of asthma induced by OVA. Methods : C57BL/6 mouse was divided into normal, NRI, control, and NRII, and Notopterygii Rhizoma Extract was orally administered to the C57BL/6 mice in the normal group and the normal mouse in NRI group while it was orally administered to the control group after inducing asthma by OVA and NRII. The study measured them by means of ELISA after dividing BALF of each group, and analyzed RT-PCR by using lung tissues. Results : In our results, lung weight and the number of total cells in the lung and of Leukocyte and Eosinophils in BALF were significantly decreased. In addition, IL-4, IL-5, IL-13, and IgE in BALF were significantly decreased compared to the control group. Conclusions : These results demonstrate that NR Extract be a desirable altemative therapy for allergic asthma by inhibiting the expressin of immune cells, the activation of inflammatory mediator.
Anticoagulant Activity of Gleditsiae Spina Extract
Yoo, Ji-Hyun ; Jung, Bung-Tea ; Kil, Gi-Jung ;
The Korea Journal of Herbology, volume 25, issue 1, 2010, Pages 39~43
Objectives : This research was investigated the anticoagulant effect of the Gleditsiae spina extract. Methods : We researched prothrombin time (PT) assay, activated partial thromboplastin time (APTT) assay in vitro and in vivo using arteriovenous (A-V) shunt rat model and shortening Rat tail bleeding time (BT). A-V shunt and BT were treated with extract of Gleditsiae spina (GS) 400 mg/kg for a week. Results : Bleeding time of Gleditsiae spina extract in vivo had a significant increase as about 1.2 times and thrombus weight of Gleditsiae spina extract had a significant reduction of thrombus weight as 26%. Gleditsiae spina extract represented an effect of anticoagulation by operating on extrinsic pathway factor II, V, VII, X and intrinsic pathway factor VIII, IX, X, XI, XII in the coagulation system. Conclusions : Considering the above mentioned results, it is judged that a Gleditsiae Spina extract has a control effect of thrombus creation.
The Comparative Study on Zanthoxylum Peel's Impurity Tolerance Based on Pharmacopoeia by Countries
Park, Su-Jin ; Kim, In-Rak ;
The Korea Journal of Herbology, volume 25, issue 1, 2010, Pages 45~54
Objectives : The purpose of this study is to determine the impurity tolerance of Zanthoxylum Peel. Methods : Compare with medicinal Herb Books and the Pharmacopoeia of 6 nations. Results : Current Pharmacopoeia show different Zanthoxylum Peel's Purity, such as 2.0% of seeds and fruit stalk, etc. in North Korea, 2% of seeds in Vietnam, totally 3% in China. On the other hand, Korea and Japan set the total number 26.0% including the specific numbers such as 20.0% of seeds, 5.0% of fruit stalk, 1.0% of the other foreign matter. This Zanthoxylum Peel's Purity, 26.0%, is too high compared to that of other medical matters specified by The Korean Pharmacopoeia Ninth Edition. When The Japanese Pharmacopoeia Sixth Edition firstly set the Zanthoxylum Peel's Purity, the herbal name was Fructus. However, since the part for medical usage in origin is well-ripen pericarp, not seed, the permissible level, 30.0%, is supposed to be simple error range, 3.0%. Conclusions : As a result, I think bills concerning the Zanthoxylum Peel's Purity should be revised to the total number 3.0% or specifically set the level 2.0% of seeds, 1.0% of fruit stalk, twig and so on.
Inhibitory Effect of a Decoction of Eucommiae ulmides OLIVER and Dipsacus asperoides C. Y. Cheng et T.M.Ai on Collagen II-induced Arthritis Mice
Lee, Boo-Kyun ; Lee, Young-Cheol ; Lee, Jang-Cheon ;
The Korea Journal of Herbology, volume 25, issue 1, 2010, Pages 55~63
Objectives : The object of this study was to verify the inhibitory effect of a decoction of Eucommiae ulmides OLIVER (EU) and Dipsacus asperoides C. Y. Cheng et T.M.Ai (DA) on Collagen II-induced Arthritis Mice (CIA mice). Methods : DBA/1OlaHsd mice were immunized with bovine type II collagen. Boostnig same collagen 21 days later, arthritis was induced and then administrated orally the extract of EU+DA (200 or 50 mg/kg) once a day for 4 weeks and compared with that of methotrexate (MTX, 0.3 mg/kg) as a positive control. Results : Administration of EU+DA suppressed the inflammatory progression of CIA mice and the results were 1. Arthritis index of CIA mice was decreased. 2. EU+DA decreased the production of TNF-
, IL-6, IL-
in the serum of CIA mice. 3. EU+DA decreased the level of IgM. 4. EU+DAincreasaed
/CD25 but decreased
in PBMC of CIA mice. 5. EU+DA decreased
of paw joint in CIA mice. 6. EU+DA decreased subsynovial inflammation. Conclusions : This results demonstrated that extract of EU+DA suppressed the inflammatory progression of CIA mice and supported further studies are required to clarify a mechanism of therapeutic role.
Inhibitory Effects of Achyranthis Bidentatae Radix on Osteoclast Differentiation and Bone Resorption
Kim, Ju-Ho ; Ki, Ji-Ye ; Ann, Ji-Young ; Park, Hye-Jung ; Kim, Hyun-Ju ; Kwak, Han-Bok ; Oh, Jae-Min ; Kim, Yun-Kyung ;
The Korea Journal of Herbology, volume 25, issue 1, 2010, Pages 65~74
Objectives : Achyranthis Bidentatae Radix (ABR) has been used for treating of many symptoms especially osteoporosis and rheumatoid arthritis. In this study, we determined the effects of water extract of ABR in RANKL (Receptor Activator for Nuclear Factor
B Ligand)-induced osteoclast differentiation culture system. Methods : We assayed mRNA expression levels of NFATc1, c-Fos, TRAP, OSCAR,
, DAP12 and GAPDH in bone marrow macrophages (BMMs) treated with ABR. The protein expression levels of NFATc1, c-Fos, MAPKs and
-actin in cell lysates treated with ABR were analysed by Western blotting. In addition we determined the effects of water extract of ABR on LPS-induced bone-loss mouse. Results : Water extract of ABR showed remarkable inhibition on RANKL-treated osteoclast differentiation without cytotoxicity. ABR down-regulated the induction of c-Fos and NFATc1 by RANKL. ABR suppressed phosphorylation of JNK, p38 and I-
. ABR rescued bone erosion by LPS induction in vivo study. Conclusions : These results demonstrate that ABR may be a useful remedy for curing of bone-loss disease such as osteoporosis.
New Multi-herb Mixture, HT005, Induces Longitudinal Bone Growth in Male Rats
Kim, Mi-Yeon ; Ramirez, Marcus J. ; Lee, Dong-Hun ; Lim, Dong-Wook ; Choi, Ho-Young ; Kim, Ho-Cheol ;
The Korea Journal of Herbology, volume 25, issue 1, 2010, Pages 75~81
Objectives : It was investigated new herbal prescription HT005 supported by traditional Korean medicine has a activity on the longitudinal bone growth of rats. HT005 were consisted of the root of Angelica sinensis, the fruit of Alpinia oxyphylla, the root of Astragalus membranaceus, the stem of Eleutherococcus senticosus, and the root of Dioscorea japonica, and Poria cocos. Method : To investigate the effect on longitudinal bone growth in adolescent male Sprague-Dawley rats, the longitudinal length of growth plate was measured directly by the tetracycline fluorescent labeling method and chondrocyte staining method. HT005 administered orally for four days, and the tetracycline was injected twice on the growth plate of the animals for fluorescent dying. The rate of longitudinal bone growth was measured the length between the tetracycline bands which fixed on the 3rd day and 5th day after injection. Cresyl violet was also used to stain the chondrocytes in the growth plate. The length of growth plate after administration was compared. Expression of IGF-1 and BMP-2 in the growth plate was investigated by immunohistochemistry. Results : HT005 showed a significant longitudinal bone growth which was
at the dose of 100 mg/kg and
(p < 0.001)at the dose of 10 mg/kg of HT005, compared to control group by the tetracycline fluorescent labeling method. HT005 showed a significant chondrocyte length on growth plate which was measured
(p < 0.001) at the dose of 100 mg/kg and
(p < 0.001)
at the dose of 10 mg/kg compared to the control group by cresyl-violet staining method. Both the number and intensity of BMP-2 and IGF-1 positive cells were increased in the hypertrophic zone of growth plate. There was a significant correlation between BMP-2 and IGF-1 expression and heights of chondrocytes in growth plate. Conclusions : Treatment of HT005 on Sprague-Dawley rats markedly increased the longitudinal bone growth. Therefore, HT005 may be an alternative herbal source to growth hormone as it promotes bone growth in children afflicted by growth retardation.
Comparative Study of Anti-oxidant and Anti-inflammatory Activities between Curcumae longae Radix and Curcumae longae Rhizoma
Oh, Hye-In ; Park, Han-Byeol ; Ju, Mi-Sun ; Jung, Sun-Yong ; Oh, Myung-Sook ;
The Korea Journal of Herbology, volume 25, issue 1, 2010, Pages 83~91
Objectives : In this study, we compared the anti-oxidant and anti-inflammatory activities of Curcumae longae Radix (CLRa) and Curcumae longae Rhizoma (CLRh). Methods : We performed 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and 2,2-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) cation scavenging assays, and determined total polyphenolic content to examine the anti-oxidant effects of CLRa and CLRh. We also evaluated the anti-oxidant effects of CLRa and CLRh against hydrogen peroxide (
)-induced toxicity in PC12 cells using thiazolyl blue tetrazolium bromide (MTT) and reactive oxygen species (ROS) assays. Next, to compare the anti-inflammatory effects of CLRa and CLRh against lipopolysaccharide (LPS)-induced inflammation in microglia BV2 cells, we measured nitric oxide (NO) assay and inducible nitrite synthase (iNOS) using Western blotting analysis. Results : CLRa showed higher activity in DPPH and ABTS assays and lower total polyphenolic contents compared with CLRh. In PC12 cells, CLRa and CLRh showed no difference in H2O2-induced cell toxicity and ROS overproduction. In BV2 cells, CLRa showed higher effect than CLRh in NO and iNOS production induced by LPS. Conclusions : These results demonstrate that CLRa has higher radical scavenging activities and anti-inflammatory effect in BV2 cells comparing CLRh. However, CLRa and CLRh have no effect and no difference in