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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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The Korea Journal of Herbology
Journal Basic Information
Journal DOI :
The Korea Association of Herbology
Editor in Chief :
Volume & Issues
Volume 26, Issue 4 - Dec 2011
Volume 26, Issue 3 - Sep 2011
Volume 26, Issue 2 - Jun 2011
Volume 26, Issue 1 - Mar 2011
Selecting the target year
Criteria for Identifying Akebiae, Clematidis, Aristolochiae Caulis
Lee, Guem-San ; Park, Kyoung-Bum ; Kim, Young-Sik ; Lee, Seung-Ho ; Oh, Hyun-Min ; Baek, Ji-Seong ; Cho, Su-In ; Kim, Hong-Jun ; Ju, Young-Sung ;
The Korea Journal of Herbology, volume 26, issue 1, 2011, Pages 1~6
Objectives : Due to morphological similarity, many substitutes and counterfeits have been used as Moktong for thousands of years. This study was designed to determine discriminative criteria for identifying medicinal herbs used as Moktong. Methods : Akebia quinata, A. trifoliata, Clematis armandii, and Aristolochia manshuriensis were collected in various regions. Samples were authenticated and examined by observation and microscopy with paraffin embedding and triple staining using the modified Ju staining method. Results : Three different types of features to establish discriminative criteria for four kinds of Moktong were identified. Botanical features include differences in phyllotaxy, leaf shape, and caulescent. External morphological features were arrangement and size of fine holes(xylem), and overall shape of the section. Internal morphological features include the number of medullary rays, shape of the pitch, type of tissues rounding pitch, appearance of annual rings, shape and amount of crystals(calcium-oxalate), and the appearance of cork cambium. Further details(e.g. identification keys) are in the article. Conclusions : These criteria could provide an effective method for identifying numerous kinds of Moktong distributed in markets throughout northeast Asian nations.
GC-MS Analysis of Chemical Constituents from Various Agarwood
Shin, Kwhang-Ho ; Choi, Kyu-Yeol ; Cho, Sung-Yong ; Ahn, Duk-Kyun ; Park, Seong-Kyu ;
The Korea Journal of Herbology, volume 26, issue 1, 2011, Pages 7~12
Objectives : The purpose of this study is to evaluate the quality of four different kinds of agarwood and analysis chemical constituents by GC-MS. Methods : Four source plants were two of Vietnamese agarwood, Indonesian agarwood and Myanmar agarwood. These plant materials were named sample No. 1-4. The chemical constituents of each agarwood were analysed by GC-MS. Results : GC-MS analysis showed that
-selinene was found in all the samples. The Vietnamese agarwood, Sample 1, and 4 has contained 6.861% and 7.497% of
-selinene and reached the highest level at the retention times 46.245 and 46.274 (min). Sample 2, the Myanmar agarwood has contained 1.608%, the lowest level of
-selinene, and it also has contained about 20% of
-eudesmol and 4.076% of valerianol. Sample 3, the Indonesian agarwood has contained 9.59% of
-selinene, 9.493% of
-selinene and shown its highest level (more than 19%) at the retention time 43.998 (min). Conclusion : It suggested that
-selinene could be a standard marker to evaluate agarwood by GC-MS analysis.
Comparative Study of Changpo(Ch
) species on Antioxidant Activity and HPLC Pattern Analysis
Choi, Go-Ya ; Ko, Byoung-Seob ; Lee, Mi-Young ; Chae, Sung-Wook ; Kim, Young-Hwa ; Ryuk, Jin-Ah ; Baek, Ji-Seong ; Lee, Hye-Won ;
The Korea Journal of Herbology, volume 26, issue 1, 2011, Pages 13~19
Objective : This study was to compare antioxidant activity and HPLC pattern analysis from 4 species of changpo(ch
). Methods : To compare the antioxidant activity and HPLC pattern analysis from the 4 species of changpo, we performed the in vitro anti-oxidative activity assays and HPLC analysis from 70% ethanol extracts of Acorus gramineus Sol. (=AG), A. tatarinowii Schott (=AT), A. calamus L. (=AC) and Anemone altaica Fisch. ex C.A.Mey (=AA) taken in the herbal medicine market of Korea. Results : AG has the most effective anti-oxidative activity among 4 species of changpo. As the HPLC pattern analysis, AT was detected the unknown peak at retention time 14.9 min whereas AG was not showed any peak at the same retention time. These results suggest that AG could be used rather than AT when it need to be prescribed as anti-oxidative medicine. Conclusions : This result can be used as the basic data contributing to the stability of AG according to an appropriate clinical application.
Study on Manual of Guideline Book for Combined Medicaion of the Oriental and the Western Drugs in the Disease of the Aged
Jeon, Won-Kyung ; Seo, Bu-Il ; Han, Chang-Hyun ; Kim, Dae-Jun ;
The Korea Journal of Herbology, volume 26, issue 1, 2011, Pages 21~27
Objectives : This study aimed to design manual of guideline book for combined medicaion of the oriental and the western drugs in the disease of the aged. Method : First, we collected informations about guideline book for drug medication, and we investigated wishes of professors who are working for an oriental medicine at universities. Result & Conclusion : 1. The greater part of professors recognized necessity of guideline book for combined medicaion of the oriental and the western drugs. 2. The basic stages of framing guideline book for combined medicaion of the oriental and the western drugs are as follows. The first step is planning of framing guideline book for combined medicaion of the oriental and the western drugs. The second step is organization of framing group and advisory committee for guideline book. The next steps are searching the core of items and making a searching all the literature and estimating the literature. And then, we should complete basic contents of guideline book in a row, and those must be examined thoroughly by external experts. Finally we can make public the guideline book for combined medicaion of the oriental and the western drugs. If there are reform contents in the guideline book, we can amend contents and make public the revision guideline book again. 3. We need guideline information center for combined medicaion of the oriental and the western drugs in the disease of the aged for framing, wide-spread and development of guideline book. 4. We think that the guideline information center should be composed of a steering committee, a committee of controlling informations and advisory committee for guideline book.
Effects of Angelica Gigantis Radix Extracts on the Collagenase Activity and Procollagen Synthesis in HS68 Human Fibroblasts and Tyrosinase Activity
Lee, Jung-Hun ; Lee, Se-Na ; Kim, Myung-Gyou ; Kim, Myoung-Hee ; Kim, Hyung-Jun ; Jo, Hak-Jun ; Leem, Kang-Hyun ;
The Korea Journal of Herbology, volume 26, issue 1, 2011, Pages 29~33
Objectives : This study was designed to investigate the collagen metabolism and tyrosinase activity of Angelicae Gigantis Radix extracts (AGR). Methods : The effect of AGR on type I procollagen production and collagenase activity in human normal fibroblasts HS68 after UVB (312 nm) irradiation was measured by ELISA method. The tyrosinase activity after treatment of AGR was measured. Results : Type I procollagen production was recovered by AGR in UVB damaged HS68 cells. The increased collagenase activity after UVB damage was significantly recovered by AGR and the tyrosinase activity was significantly reduced. However, the L-DOPA oxidation was not changed. Conclusion : AGR showed the anti-wrinkle effects and whitening effects in vitro. These results suggest that AGR may have potential as an anti-aging ingredient in cosmetic herbs.
Discrimination of Chinese Glycyrrhiza uralensis and Uzbek Glycyrrhiza glabra Using Taste Sensor
Choi, Go-Ya ; Kim, Young-Hwa ; Chae, Sung-Wook ; Lee, Hye-Won ; Ko, Byoung-Seob ; Lee, Mi-Young ;
The Korea Journal of Herbology, volume 26, issue 1, 2011, Pages 35~39
Objectives : Genetic analysis and taste pattern were performed to identify species between Glycyrrhiza uralensis and G. glabra which are officially listed in Korean Pharmacopoeia IX as origin of Gamcho(g
o, licorice root, Glycyrrhizae Radix et Rhizoma). Methods : Genetic analysis showed that identification between two species was done by comparing base sequence of ITS(intergenic transcribed spacer) and trnH-psbA regions from eleven Gamchoes sold in market. There was different taste pattern using by taste sensor in Glycyrrhiza uralensis and G. glabra. Results : Genetic analysis showed that six Gamchoes from China were identified as Glycyrrhiza uralensis and five Gamchoes from Uzbekistan were G. glabra. From the results of taste pattern, sourness and astringency of Glycyrrhiza uralensis from China were significantly higher than G. glabra from Uzbekistan, and aftertaste of astringency, aftertaste of umami, and saltiness of Glycyrrhiza uralensis were signicantly low as compared to G. glabra. There is no significant difference between two species in terms of bitterness, aftertaste of bitterness, and umami. Conclusions : Taken together, Glycyrrhiza uralensis from China and G. glabra from Uzbekistan were identified by taste sensor, and this technic could be applied to establishment of taste pattern marker for identification of different species located in various regions.
Simultaneous determination of baicalin and glycyrrhizin in Sosiho-tang by HPLC and anti-inflammatory activity
Kim, Jung-Hoon ; Ha, Hye-Kyung ; Seo, Chang-Seob ; Lee, Jin-Ah ; Shin, Hyeun-Kyoo ;
The Korea Journal of Herbology, volume 26, issue 1, 2011, Pages 41~46
Objectives : To quantitate the main compounds and investigate the biological activity of Sosiho-tang (Xiao-Chai-Hu-Tang, SST), simultaneous determination of baicalin and glycyrrhizin, and anti-inflammatory activity were estimated. Methods : A quantitative analysis was performed using a high performance liquid chromatography (HPLC). Reference compounds were separated on a reversed-phase column using gradient elution with water and acetonitrile each containing acetic acid at a flow rate of 1 mL/min. And the productions of nitric oxide (NO) and prostaglandin
were examined by lipopolysaccharide (LPS)-treated RAW 264.7 cells in the presence of the SST. The anti-inflammatory activity of SST was investigated by carrageenin-induced paw edema in rats. The paw volume was measured at 2 and 4 hr following carrageenin-induced paw edema in rats. Results : The correlation coefficients of the compounds showed good linearity (
> 0.9992) over the linear range. The precisions of intra- and inter-day were less than 7.0% of relative standard deviation (RSD) values for baicalin and less than 3.5% of RDS valuse for glycyrrhizin. Recovery rates were within the range of 95.41-101.5%. The contents of baicalin and glycyrrhizin in SST were average 70.52, 6.18 mg/g, respectively. And SST exhibited inhibitory effect on NO production in LPS-treated RAW 264.7 cells but not on
production. Oral administration of SST (1 g/kg) showed a reduction in carrageenin-induced paw edema on rats. Conclusions : The analytical method was applied successfully to measure the contents of baicalin and glycyrrhizin in SST which exhibited anti-inflammatory activities.
Effect of Leonuri Sibirici Herba and Pruni Persicae Semen On Pregnant Rats
Seo, Bu-Il ;
The Korea Journal of Herbology, volume 26, issue 1, 2011, Pages 47~52
Objectives : The present study has been undertaken to investigate the effects of Leonuri Sibirici Herba and Pruni Persicae Semen on pregnant rats. Method : In this experiment, the pregnant rats were administered by water extracts of Leonuri Sibirici Herba and Pruni Persicae Semen. The levels of weights, ALT, AST, ALP, BUN, creatinine, progesterone, Na and K in serum and reproductive indices of the rats were measured after treatment. Results : 1. The levels of body weight gains were not significantly changed in comparison with Control group in Leonuri Sibirici Herba group and Pruni Persicae Semen group. 2. In the levels of reproductive indices of the rats, the number of corpora lutea, implantation, viable fetuses, pre-implantation loss, post-implantation loss, fetal weight and placental wight were not significantly changed in comparison with Control group in Leonuri Sibirici Herba group and Pruni Persicae Semen group. 3. The levels of BUN, creatine, ALT, AST and ALP were not significantly changed in comparison with Control group in Leonuri Sibirici Herba group and Pruni Persicae Semen group. 4. The level of progesterone was not significantly changed in comparison with Control group in Leonuri Sibirici Herba group and Pruni Persicae Semen group. 5. The levels of Na and K were not significantly changed in comparison with Control group in Leonuri Sibirici Herba group and Pruni Persicae Semen group. Conclusion : Reviewing these experimetal results, it appeared that Leonuri Sibirici Herba and Pruni Persicae Semen had not toxicity on pregnant rats.
Effect of Scutellariae Radix Water Extract on Hydrogen Peroxide Production in RAW 264.7 Mouse Macrophages
Park, Wan-Su ;
The Korea Journal of Herbology, volume 26, issue 1, 2011, Pages 53~58
Objectives : The purpose of this study is to investigate effects of Scutellariae Radix Water Extract on hydrogen peroxide production in RAW 264.7 mouse macrophages. Methods : Scutellariae Radix produced from South Korea (SK) and Scutellariae Radix produced from China (SC) were extracted by hot water. Effects of SK and SC on hydrogen peroxide production in RAW 264.7 were measured by dihydrorhodamine 123 assay after 2, 4, 20, 24, 28, 44, and 48 h incubation at the concentrations of 10, 25, 50, and 100 ug/mL. Results : SK significantly increase hydrogen peroxide production in RAW 264.7 cells for 2, 4, 20, 24, 28, 44, and 48 h incubation at the concentrations of 10, 25, 50, and 100 ug/mL (P < 0.05). SC also significantly increase hydrogen peroxide production in RAW 264.7 cells for 4, 20, 24, 28, and 48 h incubation at the concentrations of 10, 25, 50, and 100 ug/mL (P < 0.05). For 2 h incubation, SC significantly increase hydrogen peroxide production in RAW 264.7 cells at the concentrations of 10, 25, and 100 ug/mL (P < 0.05). For 44 h incubation, SC significantly increase hydrogen peroxide production in RAW 264.7 cells at the concentrations of 10, 25, and 50 ug/mL (P < 0.05). Conclusions : These results suggest that Scutellariae Radix has the immune - enhancing property related with its increasement of hydrogen peroxide production in macrophages.
Effects of Astragali Radix Extracts on the Elastase Activity and DPPH and NO Scavenging Activities
Mou, Jong-Cheng ; Lee, Se-Na ; Kim, Myung-Gyou ; Kim, Myoung-Hee ; Kim, Hyung-Jun ; Jo, Hak-Jun ; Leem, Kang-Hyun ;
The Korea Journal of Herbology, volume 26, issue 1, 2011, Pages 59~63
Objectives : Elastic fibers are found in the skin, lungs, arteries, veins and other structures. The defects of elastic matrix aggravate hypertension which is associated with alteration in the great arteries, arteries, and arterioles. The elastase inhibitors were undergoing in clinical studies about emphysema and pulmonary hypertension. This study was designed to investigate the effect of Astragali Radix extracts (AR) on elastase activity and anti-oxidative effects. Methods : The elastase inhibitory activity and DPPH (1,1-diphenyl-2-picrylhydrazyl) and NO free radical scavenging activities of AR were measured. Results : The elastase activity was significantly inhibited by AR. The significant DPPH and NO free radical scavenging activities were observed in AR as well. Conclusion : AR showed the anti-elastase effects and anti-oxidative activities in vitro. These results suggest that AR may be a possible drug for the treatment of pulmonary emphysema and pulmonary hypertension.
Molecular biologic mechanism of obesity by GGEx18
Lee, Hee-Young ; Yoon, Ki-Hyeon ; Seo, Bu-Il ; Park, Gyu-Ryeol ; Yoon, Mi-Chung ; Shen, Zhi-Bin ; Cui, Hong-Hua ; Shin, Soon-Shik ;
The Korea Journal of Herbology, volume 26, issue 1, 2011, Pages 65~74
Objectives : This study was undertaken to verify the modulation mechanism of Gyeongshingangjeehwan18 (GGEx18) in ob/ob male mice. Methods : Eight-week old mice (wild-type C57BL/6J and ob/ob) were used for all experiments. Wild-type C57BL/6J mice were used as lean control and obese ob/ob mice were randomly divided into 5 groups : obese control, GGEx15 (Ephedra sinica Stapf + Rheum palmatum L.), GGEx16 (Ephedra sinica Stapf + Laminaria japonica Aresch), GGEx17 (Rheum palmatum L. + Laminaria japonica Aresch), and GGEx18 (Ephedra sinica Stapf + Laminaria japonica Aresch + Rheum palmatum L.). After mice were treated with several kinds of GGEx for 11 weeks, the mRNA expression of peroxisome proliferator-activated receptor (PPAR) target genes and uncoupling protein (UCP) were measured. In addition,
transactivation was examined in NMu2Li hepatocytes, C2C12 myocytes, and 3T3-L1 preadipocytes using transient transfection assays. Results : 1. Hepatic
target genes, such as ACOX and VLCAD mRNA levels were significantly increased by GGEx18 compared with obese controls. In skeletal muscle, LCAD mRNA expression was stimulated by GGEx16, GGEx17, and GGEx18, whereas MCAD mRNA expression by GGEx17 and GGEx18.
target LPL mRNA levels were also increased by GGEx16, GGEx17, and GGEx18 in skeletal muscle, but adipose LPL mRNA levels were decreased. In addition, GGEx18 upregulated UCP mRNA expression in skeletal muslce. 2.
reporter gene expression was increased by GGEx18 in NMu2Li cells compared with vehicle.
reporter activities were also increased by all GGEx treatments in C2C12 and 3T3-L1 cells. Conclusions : These results suggest that GGEx can act as
activators, and that GGEx may regulate obesity by stimulating
, and UCP activity. Of the 4 compositions, GGEx18 seems to be most effective in improving obesity and lipid disorders.
Effect of Samhwangsasim-tang, Samigangap-tang and Bangtan-tang on Blood Pressure in Stroke Prone Spontaneously Hypertensive Rats
Kim, Eun-Ju ; Kim, Hye-Yoom ; Lee, Jae-Yun ; Lee, Jun-Kyoung ; Kim, Seung-Ju ; Choi, Kyoung-Min ; Kang, Dae-Gill ;
The Korea Journal of Herbology, volume 26, issue 1, 2011, Pages 75~80
Objectives : The aim of the present study is to investigate the hypotensive effect of Samhwangsasim-tang (SHSST), Samigangap-tang (SMGAT) and Bangtan-tang (BTT) in stroke-prone spontaneously hypertensive rats (SHR-SP). Methods : SHR-SP rats were treated with SHSST, SMGAT and BTT at dose of 200 mg/kg/day orally for 5 weeks, respectively. Results : Treatment SHR-SP rats with SMGAT significantly lowered blood pressure but not in the SHSST or BTT treat groups. On the other hand, SHSST, SMGAT and BTT ameliorated endothelium-dependent and independent vascular relaxation in the phenylephrine-precontracted aorta and carotid artery, respectively. Conclusions : These results indicated that SMGAT has an antihypertensive effect and SHSST, SMGAT and BTT improve vascular function in stroke-prone hypertensive rat model, respectively.
Effects of Angelicae Gigantis Radix (AGR) on Polycystic Ovary induced by Estradiol Valerate in rats
Kim, Hyung-Woo ; Choi, Eu-Gene ; Chung, Hee-Jin ; Joung, Young-Min ; Shin, Dong-Sung ; Cho, Su-In ;
The Korea Journal of Herbology, volume 26, issue 1, 2011, Pages 81~85
Objective : This study was designed to investigate the effects of Angelicae Gigantis Radix (AGR) on Polycystic Ovary (PCO) induced by Estradiol valerate (EV) in female rats. Methods : We investigated the effects of AGR on Changes in body weights and food and water uptake for 5 weeks. In addition, we examined the effects on ovary weights. Finally, we also observed histopathological changes in PCO rats. Results : Treatment with AGR did not affect body weights, amount of food and water uptake. Administration group of AGR restored body/ovary weight ratio to normal levels, which were lowered by induction of PCO. In histopathological observation, formation of cysts was suppressed in AGR group compared with non-treated control group. Conclusion : These results suggest that AGR can be used for patients with PCO to prevent formation of cystic follicles and malfunction of ovary.
Anti-oxidative and anti-inflammatory effects of aerial parts of Rumex japonicus Houtt. in RAW 264.7 cells
Cho, Hyun-Jin ; Yun, Hyun-Jeong ; Park, Sun-Dong ;
The Korea Journal of Herbology, volume 26, issue 1, 2011, Pages 87~96
Objectives : The aerial parts of Rumex japonicus Houtt. (RF) is used by traditional clinics to treat parasite infection in East asia. This study aims a verification of anti-oxidative and anti-inflammatory effects of RF methanol extract. Methods : Anti-oxidative effects of RF were measured by scavenging activities of DPPH, superoxide, nitric oxide (NO) and peroxynitrite radicals. And also scavenging activities of anti-oxidation in lipopolysaccharide (LPS)-treated RAW 264.7 cells were measured. The inhibitory effects against the production of inflammatory mediators including NO, prostaglandin
), tumor necrosis factor-
), IL-6, inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and the translocation of nuclear factor (NF)-
in LPS-stimulated RAW 264.7 cells by RF were tested. Results : RF scavenged DPPH, superoxide, NO and peroxynitrite radicals, and RF (at
) reduced the inflammatory mediators definitely. Conclusions : These results indicate that RF may be a potential drug source for oxidative stress related inflammatory diseases.
A Study on the Actual Origin Species of Aquilaria malaccensis Imported from Indonesia
Kim, In-Rak ; Park, Beyung-Su ;
The Korea Journal of Herbology, volume 26, issue 1, 2011, Pages 97~101
Objectives : The purpose of this research is to identify the actual origin species imported as Aquilaria malaccensis from Indonesia. Methods : After producing the permanent preparat by cutting off the specimen thinly, we observed its characteristics. Results : The imported ones have not interxylary parenchyma, so none is in the genus aquilaria. Since the aliform parenchyma is mostly observed around the vessel pore, they are in the genus gonystylus, and the rest have no aliform parenchyma, so their specimen is unidentified. Conclusion : The imported as A. malaccensis are not in the genus aquilaria, but most of them are in the genus gonystylus and, for the rest, the specimen is unidentified.
Standardization of Eleutherococcus species and HPLC Method Validation for Quantitative Analysis
Song, Mi-Kyung ; Kim, Mi-Yeon ; Kim, Ho-Cheol ;
The Korea Journal of Herbology, volume 26, issue 1, 2011, Pages 103~110
Objective : For the standardization and quality control of eleutheroside E in Eleutherococcus species, HPLC analysis was performed and eleutherosdie E content was compared in 23 kinds of Eleutherococcus species collected from Korea and China. Methods : The content of eleutheroside E in stem bark of Eleutherococcus species collected from Korea and China were analyzed by HPLC. 0.5% phosphoric acid and acetonitrile was used as mobile solvent. Validation of HPLC analysis method was confirmed by analyzing specificity, linearity, precision and accuracy following ICH guideline. Results : Content of eleutheroside E was determined to be 1.0-1.6% and 0.5-0.8% in Korean and Chinese E. senticosus, respectively. Content of eleutheroside E in E. sessiliflorus was 0.7-1.1% and 0.2-0.4% respectively in Korean and Chinese origin. All calibration curves showed good linear regression. The method showed good precision and accuracy with intra-day and inter-day variations of 0.880-3.442% (RSD) and 0.606-3.328% (RSD), respectively, and average recovery was of 0.141-1.363% (RSD), for the eleutheroside E analyzed. Conclusion : These results might be used to establish a criterion of eleutheroside E in Eleutherococcus species.
Anti-oxidation and Anti-aging Effect of Mixed Extract from Korean Medicinal Herbs
Hwang, Jae-Gyu ; Yun, Jong-Kuk ; Han, Kil-Hwan ; Do, Eun-Ju ; Lee, Jin-Sang ; Lee, Eun-Ju ; Kim, Jong-Boo ; Kim, Mi-Ryeo ;
The Korea Journal of Herbology, volume 26, issue 1, 2011, Pages 111~117
Objectives : The present study was designed to investigate effects of mixed extract from korean medicinal herbs (MIX) on oxidation/reduction reaction-related and aging-related enzyme in vitro. Methods : We performed MTT assay, collagenase inhibition assay, elastase inhibition assay, tyrosinase inhibition assay, DPPH free radical scavenging assay, SOD-like activity and xanthine oxidase inhibition assay. Results : Recently, many studies have reported that elastin is also involved in inhibiting or repairing wrinkle formation, although collagen is a major factor in the skin wrinkle formation. The MIX showed 97% inhibition of collagenase activity, and 64% inhibition of elastase activity at 1 mg/ml concentration of MIX, next only to positive control, which indicate good efficacy for anti-wrinkle ingredient. Also it's treatment showed 34% inhibition of tyrosinase activity, to relate whitening effect, at the same dose of MIX. Antioxidant activities were determined by DPPH radical scavenging, xanthine oxidase (XO) inhibiting activity and SOD-like activity. Also these scavenging, XO-inhibiting and SOD-like activities were measured in 91%, 80%, and 63% inhibition, respectively, at a treated dose of 1 mg/ml, compare to control. Conclusions : These results suggest that possibility of mixed korean medicinal herbs as a functional ingredient for anti-wrinkle and whitening, anti-oxidation and anti-aging cosmetic formula.
Effects of Hair Tonic and Food including Korean Medicinal Herbs on Hair Growth in an Alopecia Model of C57BL/6 Mice
Choi, Hye-Min ; Hwang, Su-Jung ; Lee, Jin-Sang ; Do, Eun-Ju ; Kim, Mu-Young ; Kim, Mi-Ryeo ;
The Korea Journal of Herbology, volume 26, issue 1, 2011, Pages 119~124
Objective : This study was carried out to investigate effect of korean medicinal herbs-included hair tonic (MHT) and food (MF) on hair growth in an alopecia model of C57BL/6 mice. Methods : Six-weeks old mice were given laboratory rodent chow diet for 1 week. Then, we used a depilatory for hair removal on mice. The next day, mice were randomized and separated in 3 groups of 6 mice; normal group (N, vehicle epidermal application+normal diet-treated group), positive control group (C, 3% minoxidil sulfate epidermal application+normal diet-treated group), and the treatment group (T, HT epidermal application+ MF diet-treated group). The hair regrowth was determined by photograph, which was taken at 7, 14 and 21 days. And scoring indices, hair density and hair thickness, were evaluated by Folliscope at same day. In addition, the hair regrowth was analyzed insulin-like growth factor (IGF-1) in the dorsal skin of mice. Results : As a result, not only external appearance but also hair density and hair thickness on dorsal skin were increased more in C, T groups than in N group at 21 days with mixed treatment. Therefore, distinct regrowth pattern is appeared by treatment of herbal tonic and natural food for 21 days. Also, the expressions of IGF-1 on dorsal skin were higher in C, T groups than in N group. Conclusion : These results suggested that this herbal hair tonic and natural food has hair growth promoting activities and may be useful for treatment of bald or alopecia.
Clinical Trial to Evaluate the Efficacy of Extract of Citri Pericarpium on Serum Lipid Profiles in Subjects: a Randomized, Double-blind
Lee, Jin-Sang ; Do, Eun-Ju ; Kwak, Min-A ; Park, Hyun-Jin ; Ha, Il-Do ; Sung, Ki-Jun ; Kim, Mi-Ryeo ;
The Korea Journal of Herbology, volume 26, issue 1, 2011, Pages 125~132
Objectives : This study was performed to evaluate the efficacy of extract from Citri Pericarpium (CP) on lipid-lowering effect in semi-healthy subjects with increased serum levels of total cholesterol and triglyceride. Methods : A experimental group (n=45) and placebo group (n=46) were randomly established based on the plasma lipid profiles (triglyceride, total cholesterol etc.) in the semi-healthy subjects, then all subjects randomly took experimental or placebo capsules, which contain 1,200 mg of CP or the same dose of soluble starch per day with regular meals for 8 weeks by double-blind method. Results : One-hundred forty (140) subjects were screened, then 91 subjects enrolled in the study. No serious adverse events were reported for trial period. The CP treatment showed statistically significant decrease in serum levels of triglyceride both at 4-weeks (p<0.01) and at 8-weeks (p=0.0001). Futhermore, there was significant difference in the triglyceride levels between CP-treated and placebo group. The CP-treated but not placebo group, experienced decrease in serum levels of total cholesterol at 8-weeks. Then there was no significant difference in the total cholesterol levels between the two groups. In addition, statistically significant difference of AST, ALT,
-GT serum levels wasn't shown before and after trial in two groups. Conclusion : These results demonstrate that CP can significantly suppress the elevated triglyceride but not total cholesterol level. Therefore, CP may play a role in the improvement of blood lipid levels, as an effective functional food.
The Antioxidant Activity of Sesami Semen Nigrum on Leydig TM3 cells
Chang, Mun-Seog ; Chung, Kyu-Jin ; Chang, Won-Kyu ; Park, Seong-Kyu ;
The Korea Journal of Herbology, volume 26, issue 1, 2011, Pages 133~138
Objectives : The purpose of this study was to estimate the antioxidant activity of Sesami Semen Nigrum extract (SSN) on mouse Leydig cells, TM3. Methods : Cell viability assays were performed. The protective effects of SSN against hydrogen peroxide-induced oxidative stress in Leydig cells were examined by measuring cell viability. Lipid peroxidation levels and antioxidant enzyme concentrations such as SOD and catalase were measured. Results : Cell viability of Leydig cells increased with SSN concentration. Cell viability of Leydig cells was 136.66% when SSN concentration was
. Cell viability of the hydrogen peroxide group was statistically decreased (p<0.01) compared with the control group. Antioxidant effect of SSN was measured and the protective effect of SSN concentration were 5, 10,
. LPO were decreased significantly at 5,
of SSN concentrations. SOD activity was increased at 1, 10,
of SSN concentrations. Catalase activity was significantly increased at 123.7, 133.3 and 131.9 units/mg protein when SSN concentrations were 5, 10 and
, respectively. Conclusions : In conclusion, Sesami Semen Nigrum extract has antioxidant activities in Leydig cells against oxidative stress.