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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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The Korea Journal of Herbology
Journal Basic Information
Journal DOI :
The Korea Association of Herbology
Editor in Chief :
Volume & Issues
Volume 31, Issue 5 - Sep 2016
Volume 31, Issue 4 - Jul 2016
Volume 31, Issue 3 - May 2016
Volume 31, Issue 2 - Mar 2016
Volume 31, Issue 1 - Jan 2016
Selecting the target year
Research of Efficacy of Curculiginis Rhizoma aquaous extract on collagen induced arthritis
Seo, Bu Il ; Roh, Seong Soo ; Park, Ji Ha ; Park, Chan Ik ; Koo, Jin Suk ;
The Korea Journal of Herbology, volume 31, issue 4, 2016, Pages 1~10
DOI : 10.6116/kjh.2016.31.4.1.
Objectives: In Korean medicine, Curculiginis Rhizoma was treated for arthritis in remedy. But efficacy of Curculiginis Rhizoma on collagen induced arthritis was not revealed.Methods: Anti inflammatory effect of Curculiginis Rhizoma was researched in vitro with RAW264.7 cell and cell toxicity, levels of proinflammatory cytokines (TNF-α, IL-1β, IL-6 and IL-12) and PGE2 were analyzed by ELISA assay. Inflammatory protein were analyzed by western blotting assay (JNK, ERK, COX-2, TNF-α and IL-1β). In vivo, collagen induced arthritis mice model was used to evaluate anti-inflammation effect through arthritis index, immune cell number and cytokine levels (TNF-α, IL-6 and IL-1β) in serum.Results: ECR(Extract of Curculiginis Rhizoma) has not shown cell toxicity in 200 ㎍/㎖ on RAW264.7 cell. ECR suppressed releases of NO, TNF-α, IL-1β, IL-6, IL-12 and PGE2 on RAW264.7 cell treated with lipopolysacharide (1 ㎍/㎖). And ECR inhibited regulation of TNF-α, IL-1β and IL-6 mRNA, reduced protein release of JNK, ERK, iNOS, COX-2, IL-1β and TNF-α. AI of group treated with ECR 200 ㎎/㎏ and 100 ㎎/㎏ were significantly decreased compared to vihicle arthritis mice, the number of immune cell in foot joint was increased on control mice but those of group treated with ECR 200 ㎎/㎏ and 100 ㎎/㎏ were significantly reduced. This results correspond with contens of cytokines (TNF-α, IL-1β and IL-6) in serum.Conclusions: Curculiginis Rhizoma has anti-inflammation effect on RAW264.7 cell in vitro and collagen induced arthritis in vivo. So it is necessary to research more mechanism for cascade imfact.
The Study on anti-obesity of Myrrh ethanol extract
Baek, Seon-Jae ; Kim, Dong-Hee ;
The Korea Journal of Herbology, volume 31, issue 4, 2016, Pages 11~18
DOI : 10.6116/kjh.2016.31.4.11.
Objectives : The objective of this study was to investigate the effect of Myrrh 80% ethanol extract on adipocyte differentiation and adipogenesis in 3T3-L1 cell.Methods : Myrrh was prepared by extracting with 80% ethanol. Cell viability was assessed by MTT assay using 3T3-L1 cells. Anti-obesity activity was measured in lipid droplets and triglyceride (TG) accumulation in 3T3-L1 cells. We also analyzed the expression of C/EBPβ, C/EBPα, PPARγ, SREBP1c, and aP2 by reverse transcriptase polymerase chain reaction (RT-PCR). In addition, we observed the production of fatty acid, acetyl-CoA carboxylase and Oil-red O stainingResults : No cytotoxicity from Myrrh 80% ethanol extracts was observed at the concentration of 1, 10, 100 (㎍/㎖) in 3T3-L1 cells. Treatment with Myrrh significantly suppressed the terminal differentiation of 3T3-L1 in a dose-dependent manner, as confirmed by a decrease in triglyceride and Fatty acid and Acetyl-CoA carboxylase. Also, Myrrh exhibited potential adipogenesis inhibition and downregulated the expression of pro-adipogenic transcription factors, such as sterol regulatory element binding protein-1c (SREBP-1c), peroxisome proliferator-activated receptor-γ (PPARγ), CCAAT/enhancer binding proteins α (C/EBPα) and C/EBPβ, and adipocyte expressed genes, such as adipocyte fatty acid binding protein (aP2) and Fas. In addition, lipid accumulation determined by Oil-red O staining showed that Myrrh extract had inhibitory effects on lipid accumulation in 3T3-L1 cells.Conclusions : These results suggest that Myrrh suppresses obesity factors in 3T3-L1 cells. Myrrh may be a useful medical herbs for attenuating metabolic diseases such as obesity.
A Herbological Study on the Plants of Nymphaeaceae in Korea
Jeong, Jong-Gil ;
The Korea Journal of Herbology, volume 31, issue 4, 2016, Pages 19~25
DOI : 10.6116/kjh.2016.31.4.19.
Objective : For the purpose of developing Korean herbology of the plants to Nymphaeaceae in Korea, the literatures of the successive generations have been thoroughly investigated to prepare this article.Methods : Books and research papers about Herbology which published at home and abroad were examined.Results : A list was made about Nymphaeaceae plant which were cultivated or grew naturally in Korea, after investigated the data on domestic and foreign Nymphaeaceae plants. Out of those lists, serviceable plants were selected and with those available plants, their distributions were analysed and parts which can be used as medicines were divided into 3 categories such as oriental medicine name, scientific name and non-official name. Nymphaeaceae's properties flavor, channels they use, effects, and toxicity were also noted. Nymphaeaceae (grew in Korea) were divided into 6 classes with 10 species. Out of those, 5 classes with 5 species were found serviceable which indicates 50% of all. Out of all 10 species of Nymphaeaceae family, Nuphar genus were found 4 species, which were shown the most. Among 5 genus of Nymphaeceae species, medicinal plants were all one species each. Out of all serviceable parts in Nymphaeaceae, Etc parts took first place as 10 species. There are no toxic, the survey said.Conclusion : There were totaled to 6 genera and 10 species in Dioscoreaceae in Korea and among them medicinal plants are 5 genera, 5 species, some 50% in total.
A Herbological Study on the Plants of Fumariaceae in Korea
Jeong, Jong-Gil ;
The Korea Journal of Herbology, volume 31, issue 4, 2016, Pages 27~33
DOI : 10.6116/kjh.2016.31.4.27.
Objective : For the purpose of developing Korean herbology of the plants to Fumariaceae in Korea, the literatures of the successive generations have been thoroughly investigated to prepare this article.Methods : Books and research papers about Herbology which published at home and abroad were examined.Results : A list was made about Fumariaceae plant which were cultivated or grew naturally in Korea, after investigated the data on domestic and foreign Fumariaceae plants. Out of those lists, serviceable plants were selected and with those available plants, their distributions were analysed and parts which can be used as medicines were divided into 3 categories such as oriental medicine name, scientific name and non-official name. Fumariaceae's properties flavor, channels they use, effects, and toxicity were also noted. Fumariaceae (grew in Korea) were divided into 3 classes with 23 species. Out of those, 2 classes with 9 species were found serviceable which indicates 39% of all. Out of all 23 species of Fumariaceae family, Corydalis genus were found 21 species, which were shown the most. Among Fumariaceae species, Corydalis genus were found 8 medicinal plants, which were shown the most. Out of all serviceable parts in Fumariaceae, Herba and Tuber parts took first place as 5 species. There are 2 toxic species and 3 toxic medicinal parts, the survey said.Conclusion : There were totaled to 3 genera and 23 species in Fumariaceae in Korea and among them medicinal plants are 2 genera, 9 species, some 39 in total.
Anti-Oxidative Effect of Jeokbaekhaogwanjoong-tang Distillate on Spleen Cells of Aged Rats
Park, Young-Chun ; Kim, Il-Gu ; Kim, Tae-Min ;
The Korea Journal of Herbology, volume 31, issue 4, 2016, Pages 35~41
DOI : 10.6116/kjh.2016.31.4.35.
Objective : The purpose of this study is to investigate the anti-oxidative effect of Jeokbaekhaogwanjoong-tang (JGT) distillate in spleen cells of aged rats.Methods : This experiment was performed using spleen cells of 10w, 52w, 72w old sprague dawley (SD) rats. The spleen cells of 10, 52, 72 weeks old rats were divided into three groups; DW group, Vit. C group, and JGT group. The cells of DW group were treated with distilled water. The cells of Vit.C group were treated with Vitamin C. And the cells of JGT group were treated with JGT distillate. After the treatment of DW, Vit.C, and JGT, the levels of superoxide dismutase (SOD), glutathione (GSH), nitric oxide (NO) and lipid peroxidation (MDA) were measured.Results : In the spleen cells of 72 weeks old rats, SOD activity was significantly increased in the JGT group compared to those in the DW group and the Vit.C group. In the spleen cells of 52 weeks old rats, GSH activity was significantly increased in the JGT group compared to the Vit.C group, and NO concentration was significantly decreased in the JGT group compared to the DW group and the Vit.C group. MDA concentration was not changed significantly in any cell groups by the treatment of JGT distillate.Conclusions : Jeokbaekhaogwanjoong-tang (JGT) distillate may have an anti-oxidative effects in the spleen cells of aged rats. Further investigation is needed for the anti-aging effect of JGT.
Effect of Original Kyungokgo & Iksuyongjingo plus Sparassis crispa on Antioxidant, Immunity Improvement and Sensory Evaluation
Na, Chang-Su ; Shin, Wook ; Lee, Yu-Mi ; Moon, Yang-Seon ; Noh, Hee-kyung ; Seo, Seong-Ho ; Son, Hong-Seok ;
The Korea Journal of Herbology, volume 31, issue 4, 2016, Pages 43~51
DOI : 10.6116/kjh.2016.31.4.43.
Objectives : This study was conducted with objectives of comparatively investigating effects of original Kyungokgo and Iksuyongjingo plus Sparassis crispa which is gamibang of Kyungokgo on antioxidant improvement, immunity improvement, and sensory evaluation.Methods : Total phenol and DPPH scavenging activity were measured to determine antioxidant improvement of original Kyungokgo and Iksuyongjingo plus Sparassis crispa. While, blood and serum study were conducted and effect on the cytokines were observed from the immunosuppressed rat induced by methotrexate (MTX). Control group was administered with a drinking water, Kyungokgo group was administered with a original Kyungokgo 200 mg/kg, and Iksuyongjingo plus Sparassis crispa group was administered with a Iksuyongjingo plus Sparassis crispa 200 mg/kg for 20 days. Further, intensity and preference evaluation were performed as sensory evaluation.Results : It was observed that Iksuyongjingo plus Sparassis crispa significantly increased total phenol and DPPH scavenging activity, proving that these can improve antioxidant activities. In the immunosuppressed rat, Iksuyongjingo plus Sparassis crispa significantly decreased IL-2 level, significantly increased Il-10 level, and significantly increased neutrophils, RBC, HGB, and HCT levels, indicating that it is effective in increasing immunity. Further, Iksuyongjingo plus Sparassis crispa significantly improved intensity and preference in the sensory evaluation.Conclusions : From the above results, it is concluded that Iksuyongjingo plus Sparassis crispa which is gamibang of Kyungokgo can act effectively on improving antioxidant activity and immunity, and can attribute convenient intake by improving diet preference.
Antioxidant and Anti-wrinkling Effects of Extracts from Nelumbo nucifera leaves
Park, Chan-Ik ; Park, Geun-Hye ;
The Korea Journal of Herbology, volume 31, issue 4, 2016, Pages 53~60
DOI : 10.6116/kjh.2016.31.4.53.
Objective : The purpose of this study was to investigate anti-aging and antioxidant effects of extracts of Nelumbo nucifera leaves (NN-L) using ethanol on skin .Methods : Each part of leaves(NN-L), flowers(NN-F) and stem(NN-S) was extracted with 70% ethanol. We performed radical scavenging assay(DPPH, ABTS+, Superoxide anion radical), elastase inhibition assay, collagenase inhibition assay. NN-L extracts were tested for cell viability(MTT assay), MMP-1 inhibition and MMP-1 protein expression on CCD-986sk cells (human fibroblast line).Results : Recently, many studies have reported that elastin is also involved in inhibiting or repairing wrinkle formation, although collagen is a major factor in the skin wrinkle formation. We measured its free radical scavenging activity, elastase inhibitory activity and expression of MMP-1 (matrix metalloprotease-1) in human fibroblast cells. Among the parts of Nelumbo nucifera, NN-L showed the highest antioxidant activities and in radical scavenging. DPPH, ABTS+ and Superoxide anion radical scavenging activity of NN-L at concentration of 1,000 μg/mL were 91.43%, 99.31% and 73.7% respectively. In vitro elastase and collagenase inhibition effects of NN-L at concentration of 1,000 μg/mL was 42.8% and 55.3% respectively. The ethanol extract of NN-L showed cell viability of 95.4% in 50 μg/mL concentration. In addition, The results from Western blot assay showed that NN-L decreased the expression of MMP-1 protein in a dose-dependent manner (by up to 35.0% at 50 μM).Conclusion : The findings suggest that the NN-L great potential as a cosmeceutical ingredient with antioxidant and anti-wrinkling effects.
Identification of Morphological Appearance of Minute Seed Herbs Using Stereoscope (Report II) - Alli Tuberosi Semen✳Alli Fistulosi Semen, Malvae Semen✳Abutili Semen, Plantaginis Semen✳Schizonepetae Semen
Kim, Young-Sik ; Ju, Young-Sung ;
The Korea Journal of Herbology, volume 31, issue 4, 2016, Pages 61~69
DOI : 10.6116/kjh.2016.31.4.61.
Objectives : This study is to propose discriminative keys with stereoscope for minute seed herbs easily mixed up, but hard to discriminate by naked eyes: Alli Tuberosi Semen(ATS)✳Alli Fistulosi Semen(AFS), Malvae Semen(MS)✳ Abutili Semen(AS), Plantaginis Semen(PS)✳Schizonepetae Semen(SS).Objectives : We reviewed the description of original plants and medicinal herbs from literature. The specimen of original plant were collected, determinated and compared to samples in the market. Primary classification was performed with naked eyes. and we found out other discrimination keys for non-distinctive herbs with stereoscope. Discrimination keys were set as the morphological criteria of authentic herbs, percentage of adulteration, and standards for discriminating genuine herbs from adulteration.Results : 1) ATS is bigger, has reticulate pattern on protuberant side and unique garlic chives taste. AFS which is usually mixed up, is smaller, has 1~2 ridges and unique welsh onion taste. 2) MS is smaller, has no villus, but its length of bumps are similar. AS easily mixed up, is bigger and has white villus. Its upper side bump is longer and more sharp. 3) PS has dent at middle of the back side. It becomes very sticky when it is put or boiled in water. SS which can be mixed up has no dent and low viscosity compared to PS.Conclusions : With this result, we propose discriminative keys which can identify the original plants and processed herbal state of six herbs. Because minute medicinal herbs are hardly distinguishable by sensory test, It is essential to differentiate by using stereoscope.
Anti-obese Function of Polysaccharides derived from Korean Ginseng (Panax ginseng C.A. Meyer) and Development of Functional Food Material in Preventing Obesity
Shon, Myung-Soo ; Kim, Gyo-Nam ;
The Korea Journal of Herbology, volume 31, issue 4, 2016, Pages 71~77
DOI : 10.6116/kjh.2016.31.4.71.
Objectives : Adipogenesis was defined as a differentiation process of preadipocytes into the adipocytes. Thus, to control of this process can be one of the most important strategies to prevent obesity. Korean ginseng(Panax ginseng C.A. Meyer) is one of the most widely used medicinal herbs. Although multiple biological activities of Korean ginseng, particularly ginsenosides, have been known, the anti-adipogenic role and function of polysaccharides from Korean ginseng are still unclear. In this study, we examined anti-adipogenic activity of polysaccharides and its molecular basis mechanisms are further investigated.Methods : The cytotoxicity of KGP in 3T3-L1 was evaluated by MTT assay. Anti-adipogenic effect of KGP was examined by Oil Red O (ORO) staining and microscopy observation in 3T3-L1 mature adipocytes. The mRNA expression levels of adipogenic transcriptional factors were analyzed by reverse transcription-polymer chain reaction (RT-PCR). To elucidate the adipogenic molecular mechanism of KGT, SB431542 (TGF-β specific inhibitor) was used.Results : We found that polysaccharides showed no effect on the viability of 3T3-L1 preadipocytes. Dose dependent inhibitory effect of polysaccharides on 3T3-L1 adipogenesis was observed as judged by ORO staining and microscopic image analysis. To obtain further mechanistic insight into anti-adipogenic function of polysaccharides, we then tested the effect of polysaccharides treatment on the adipogenic marker genes. The mRNA expressions level of C/EBPα, PPARγ, C/EBPβ, and fatty acid synthase (FAS) were dose-dependently inhibited by KGP treatment in 3T3-L1 mature adipocytes.Conclusions : In conclusion, these findings suggest that the KGP could be used in treatment of obesity and overweight related diseases.
Effects of Ato-tang on DNCB-induced atopic dermatitis in Mice
Kim, Gun-Woo ; Kim, Dong-Hee ;
The Korea Journal of Herbology, volume 31, issue 4, 2016, Pages 79~85
DOI : 10.6116/kjh.2016.31.4.79.
Objectives : The aim of this study is to investigate anti-atopic dermatitis effect using ato-tang.Methods : Ato-tang was external treatment to NC/Nga mice for 4 weeks, where atopic dermatitis was induced by DNCB at 1% and 0.4% for 3 weeks. Atopic dermatitis index score was measured using eye observation and picture evaluation. The histopathological change of dorsal skin was observed by hematoxylin and eosin (H&E) staining. Cytokines including IL-4, IL-5 and IL-13 were measured by Luminex or reverse transcriptase polymerase chain reaction (RT-PCR) analysis and immunoglobulin E (IgE) was measured by ELISA reader.Results : The dorsal skin of Ato-tang group showed decrease in erythema, pruritus, dry skin, edema, excoriation, erosion and lichenification level through naked eye observations. Immunoglobulin cell infiltration and the thickness of epidermis were significantly decreased in the dorsal skin compared to control. Production of Th2 cytokines (IL-4, IL-5, IL-13) and IgE level in serum were all significantly decreased, in comparison with control. In addition, mRNA expression level of Th2 cytokines (IL-4, IL-5, IL-13) in spleen was decreased, in comparison with control.Conclusion : The results indicated that external treatment of ato was improved skin barrier function in the symptoms of atopic dermatitis disease. Also, atopic dermatitis factors where cytokine as well as immunoglobulin E in serum and mRNA expression were decreased, respectively, in comparison with control. Therefore, we suggest that ato could be effectively used as a external therapeutic drug based on atopic dermatitis factors.
Anti-inflammation effect of extract from Zostera marina using UVB-induced damage on keratinocytes
Kim, Bo-Ae ;
The Korea Journal of Herbology, volume 31, issue 4, 2016, Pages 87~91
DOI : 10.6116/kjh.2016.31.4.87.
Objectives : In order to confirm whether extracts of different parts of Zostera marina (ZM), a marine flowering plant, can be used as cosmetic ingredients, this study evaluated their cytotoxicity and cytoprotective effects against ultraviolet B (UVB). Inflammatory responses induced by UV stimuli are also associated with the aging of the skin.Methods : We investigated the effects of ZM extracts on cells through the water soluble tetrazolium salt-1(WST-1) assay for cell viability. In order to investigate the anti-inflammatory effects, we evaluated the suppression of Cyclooxygenase-2 (COX-2) expression by ZM extracts in HaCaT cells with UVB-induced damages, and also evaluated the production of Prostaglandin E
) in RAW 264.7 cells with LPS-induced damages.Results : High cell viabilities above 90% were observed in all types of ZM extracts, except for whole ZM extract at 0.5 mg/ml; in keratinocytes with UVB-induced damages, the cell viabilities were above 80% when treated with all types of ZM extracts. We confirmed their anti-inflammatory effects by investigating the suppression of inflammatory mediators. In keratinocytes with UVB-induced damages, COX-2 expression decreased in the experimental group treated with ZM extract. Similarly, in RAW 264.7 cells where inflammation was induced with LPS, the biosynthesis of PGE
was inhibited.Conclusion : These results suggest that ethanol extracts from Zostera marina may have value as the potential anti-inflammatory medicinal plant. Also based on the abovementioned results, ZM extract protects skin cells from UV-induced damages, and thus can be used in topically applied products for skin protection.
A study of Search trends about herbal medicine on online portal
Lee, Seungho ; Kim, Anna ; Kim, Sanghyun ; Kim, Sangkyun ; Seo, Jinsoon ; Jang, Hyunchul ;
The Korea Journal of Herbology, volume 31, issue 4, 2016, Pages 93~100
DOI : 10.6116/kjh.2016.31.4.93.
Objectives : The internet is the most common method to investigate information. It is showed that 75.2% of Internet users of 20s had health information search experience. So this study is aim to understanding of interest of public about the herbal medicine using internet search query volume data.Methods : The Naver that is the top internet portal web service of the Republic of Korea has provided an Internet search query volume data from January 2007 to the current through the Naver data lab (http://datalab.naver.com) service. We have collected search query volume data which was provided by the Naver in 606 herbal medicine names and sorted the data by peak and total search volume.Results : The most frequently searched herbal medicines which has less bias and sorted by peak search volume is 'wasong (와송)'. And the most frequently searched herbal medicines which has less bias and sorted by total search volume is 'hasuo (하수오)'.Conclustions : This study is showed that the rank of interest of public about herbal medicines. Among the above herbal medicines, some herbal medicines had supply issue. And there are some other herbal medicines that had very little demand in Korean medicine market, but highly interested public. So it is necessary to monitor for these herbal medicines which is highly interested of the public. Furthermore if the reliability of the data obtained on the basis of these studies, it is possible to be utilizing herbal medicine monitoring service.
Neuroprotective effects of herbal mixture HT070 on global cerebral ischemia in rats
Song, Jungbin ; Lee, Donghun ; Kim, Young-Sik ; Lee, Hyun Jeong ; Lee, Seunggyeong ; Kim, Dong Kuk ; Kang, Shin Ho ; Shin, Yong Kook ; Choi, Ho-Young ; Kim, Hocheol ;
The Korea Journal of Herbology, volume 31, issue 4, 2016, Pages 101~109
DOI : 10.6116/kjh.2016.31.4.101.
Objectives : HT070 is a mixture of herbal extracts from root of Scutellaria baicalensis and stem bark of Eleutherococcus senticosus , which have long been used for stroke therapy in traditional Korean Medicine. The purpose of this study was to investigate the neuroprotective effects of HT070 on global cerebral ischemia and its potential mechanisms.Methods : Transient global cerebral ischemia was produced by 10 min of four-vessel occlusion (4-VO) in male Wistar rats. HT070 was administered orally at a dosage of 200 mg/kg twice at 0 and 90 min after reperfusion. Hippocampal neuronal damage was measured 7 days after reperfusion. To explore the potential mechanisms, we used hydrogen peroxide (H
)-induced rat pheochromocytoma (PC12) cells as an in vitro model. PC12 cells were pretreated with HT070 for 1 h and then exposed to 100 μM H
for 6 h in the presence of HT070. Cell viability was measured by MTT assay and the mRNA expression of Bax, Bcl-2, iNOS and COX-2 were measured by quantitative RT-PCR.Results : Oral administration of HT070 at a dose of 200 mg/kg significantly reduced neuronal death in the hippocampal CA1 region by 13.4% as compared to the vehicle-treated group. HT070 increased cell viability, reversed the down-regulated Bcl-2 mRNA level, and suppressed the up-regulated mRNA expressions of Bax, iNOS, and COX-2 in H
-treated PC12 cells.Conclusions : HT070 protects against delayed neuronal death after global cerebral ischemia and its neuroprotection properties might be attributed to the inhibition of mitochondrial apoptosis and ROS-generating enzymes.