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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Pharmacopuncture
Journal Basic Information
Journal DOI :
KOREAN PHARMACOPUNCTURE INSTITUTE
Editor in Chief :
Volume & Issues
Volume 12, Issue 4 - Dec 2009
Volume 12, Issue 3 - Sep 2009
Volume 12, Issue 2 - Jun 2009
Volume 12, Issue 1 - Mar 2009
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Monitoring the Change of Protein Expression in Human Colon Cancer Cell SNU-81 treated with the Water-Extract of Coptis japonica
Yoo, Tae-Mo ; Kim, Byung-Soo ; Yoo, Byong-Chul ; Yoo, Hwa-Seung ;
Journal of Pharmacopuncture, volume 12, issue 1, 2009, Pages 5~12
DOI : 10.3831/KPI.2009.12.1.005
Background : Anticancer effects of herbal medicine have been reported in various types of cancer, but the systematic approaches to explain molecular mechanism(s) are not established yet. Objective : To find the anticancer-effect and mechanism(s) of Water Extract of Coptis japonica (WECJ) colon cancer cell (SNU-81). Methods : We first selected 11 herbals, and anti-cancer effects of water-extracts from those herbals have been tested in human colon cancer cell line, SNU-81. Among the tested herbals, the WECJ significantly reduced proliferation of SNU-81. To establish a basis of understanding for anti-cancer mechanism, whole proteins have been obtained from SNU-81 harvested at 48 and 96 hrs after the treatment of WECJ, protein expression has been profiled by 2DE-based proteomic approach. Results : Various changes of the protein expression have been monitored, and most frequent dysregulation was found in the molecular chaperons including heat shock protein 90-alpha (Hsp90-alpha), 14-3-3 protein epsilon, T-complex protein 1 subunit alpha, protein disulfide-isomerase A3, and calreticulin. Interestingly, proliferation-associated protein 2G4 has been up-regulated, and it suggests the possible effect of Coptis japonica on ErbB3-regulated signal transduction pathway and growth control of human colon cancer cells. Conclusion : Based upon the present findings, the further study will focus on monitoring various cancer survival factors after artificial regulation of the proteins identified, and it would be the basis for the understanding of the Coptis japonica anti-cancer effect(s) at the molecular level.
Identification of Fel ursi and Cattle and Pig Bile Juices by speciesspecific PCR and PCR-RFLP
Kwon, Ki-Rok ; Baek, Seung-Il ; Choi, Suk-Ho ;
Journal of Pharmacopuncture, volume 12, issue 1, 2009, Pages 13~20
DOI : 10.3831/KPI.2009.12.1.013
Objective : This study developed species-specific PCR and PCR-RFLP to detect the adulteration of Fel ursi products with cattle and pig bile juices. Methods : All the primers for PCR and PCR-RFLP in this study were designed based on nucleotide sequences of cytochrome b genes in the mitochondria. Results : The species-specific PCR amplified a DNA fragment of 214, 214, 295, and 167 bp from Fel ursi product, bear fur, cattle bile juice, and pig bile juice, respectively. The survey using the speciesspecific PCR indicated that some of commercial Fel ursi products were adulterated with cattle and pig bile juices. PCR-RFLP using the restriction endonucleases, HaeIII and HinfI enabled differentiation among Fel ursi product, cattle bile juice, and pig bile juice. Bear furs from two animals showed variations in PCR-RFLP patterns with HaeIII. Discussion : The detection methods of the species-specific PCR and PCR-RFLP could be useful in eliminating adulterated Fel ursi products from the market.
The Experimental study of safety and efficacy in using Soyeom Pharmacopuncture solution as eyedrops
Kang, Eun-Kyo ; Seo, Hyung-Sik ;
Journal of Pharmacopuncture, volume 12, issue 1, 2009, Pages 21~33
DOI : 10.3831/KPI.2009.12.1.021
Objective : This experimental study was performed to investigate the safety of Soyeom Pharmacopunture solution manufactured by extraction of alcohol and water. To identify the use of it as eyedrops, the eye irritation test of rabbits and antibacterial test of Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Aspergillus niger, Fusarium oxysporum, and Candida albicans was performed. Methods : 1. The eye irritation test of this material was performed according to the Regulation of Korea Food & Drug Administration(2005. 10. 21, KFDA 2005-60). After Soyeom pharmacopuncture solution was administered in the left eye of the rabbits, eye irritation of the cornea, iris and conjunctiva was observed at 1, 2, 3, 4 & 7day. 2. After administering Soyeom Pharmacopuncture solution on bacterial species (Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Aspergillus niger, Fusarium oxysporum, Candida albicans) which cause Keratitis, MIC(Minimum Inhibition Concentration) and the size of inhibition zone were measured. Anti-bacterial potency was also measured using the size of inhibition zone. Results : 1. After Soyeom pharmacopuncture solution was administered in the left eye of the rabbits, it was found that none of nine rabbits have abnormal signs and weight changes. 2. After Soyeom pharmacopuncture solution was medicated in the left eye of the rabbits, no eye irritation of the cornea, iris and conjunctiva was observed at 1, 2, 3, 4 & 7day. 3. There was no response to MIC on bacterial species (Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Aspergillus niger, Fusarium oxysporum, Candida albicans) after Soyeom pharmacopuncture solution was medicated. Conclusions : The present study suggests that Soyeom pharmacopuncture solution is a non-toxic and non-irritant medicine, which does not cause eye irritation in rabbits, but dosen`t have anti-bacterial effects on bacterial species which cause Keratitis. These study result recommends that more research on other herbal medicines of eye drop for Keratitis are required.
Effects of bee venom acupuncture on heart rate variability, pulse wave, and cerebral blood flow for types of Sasang Constitution
Lee, Sang-Min ; Kim, Koo ; Oh, Seung-Yun ; Kwon, Young-Mi ; Joo, Jong-Cheon ;
Journal of Pharmacopuncture, volume 12, issue 1, 2009, Pages 35~42
DOI : 10.3831/KPI.2009.12.1.035
1. Objectives To evaluate effects of bee venom acupuncture on cardiovascular system and differences according to each constitution. 2. Methods Heart rate variability, pulse wave and the velocity of cerebral blood flow were measured before bee venom acupuncture(BVA), right after and after 30 minuets, had been applied to 20 subjects. 3. Results 1. BVA did not have effects on measurement variables of heart rate variability. 2. BVA had effects on pulse wave, showing total time, radial augmentation index up and height of percussion wave, time to percussion wave, sum of pulse pressure down. 3. BVA did not have effects on the cerebral blood flow velocity when considering not Sasang Constitution. 4. Considering Sasang Constitution, BVA demonstrates different responses in time to preincisura wave, mean blood flow velocity, peak systolic velocity and end diastolic velocity. 4.Conclusion From those results, the following conclusions are obtained. Cause BVA alters pulse wave and makes differences in the cerebral blood flow velocity according to Sasang Constitution. Various methods of BVA treatment are needed considering Sasang Constitution.
A Study of Ginger Herbal Pharmacopuncture for Practical Application
Lee, Chae-Woo ; Lee, Byung-Hoon ; Youn, Hyoun-Min ;
Journal of Pharmacopuncture, volume 12, issue 1, 2009, Pages 43~51
DOI : 10.3831/KPI.2009.12.1.043
Objective : The purpose of this study is to present the standard for practical application of ginger herbal pharmacopuncture Material and Methods : We refer to ancient literatures and the recent papers for ginger. Conclusions : The following results have been obtained 1. The effect of ginger(Zingiber officinale Roscoe) is to "release exterior", "balance nutrient & defensive qi", "resolve phlegm", "arrest coughing", "warm the lungs". So ginger herbal pharmacopuncture can be applied to treating fever, chilling sign, headchae, snuffle and gasping cough due to cold affection and treating the symptoms like sputum and asthma that be revealed by pulmonary disease. 2. The effect of ginger is to "warm spleen and stomach", "arrest vomiting" "promote normal flow of water". So ginger herbal pharmacopuncture can be applied to treating nausea, vomiting, abdominal distension and diarrhea due to phlegm & dampness and treating edema. 3. The effect of ginger is to eliminate blood stasis. So ginger herbal pharmacopuncture can be applied to treating contusion, blood stasis, sprain and gynecologic disease. 4. Ginger can treat myalgia and pain due to wind-damp and have anti-inflammatory effect in pharmacology. So ginger herbal pharmacopuncture can be applied to treating disease of joint, ligament and muscle. 5. Ginger can resolve phlegm and resuscitate. So ginger herbal pharmacopuncture can be applied to treating unconsciousness. But, treating incipient cardiovascular accident, it needs to call your special attention to the danger of blood pressure increase. 6. In pharmacology, ginger is effective for antitumor, antioxidant effects and activating immunocyte. So ginger herbal pharmacopuncture can be applied to treating broadly varieties of tumor and allergic disease.
A pilot clinical study on the Traditional Korean Medicine treatment of Amyotrophic lateral sclerosis
Kim, Sung-Chul ; Na, Won-Min ; Lim, Na-Ra ; Lee, Do-Sang ; Jang, Eun-Ha ; Song, Bong-Keun ;
Journal of Pharmacopuncture, volume 12, issue 1, 2009, Pages 53~65
DOI : 10.3831/KPI.2009.12.1.053
Objectives : This study was to investigate the effect of Oriental medical treatment on ALS. Methods : We investigated 12 ALS patients which were admitted to Gwang-Ju O.M. hospital from Oct. 14, 2008 to Nov. 14, 2008. All patients were treated by SAAM-acupuncture, herb medication, Bee venom Pharmacopuncture therapy, Needle-embedding therapy, etc. We evaluated patients using the Amyotrophic lateral sclerosis Functional Rating Scale-Revised(ALSFRS-R), Medical Research Council (MRC) Scale. Results : After 30 days, mean ALSFRS-R score of patients was improved from
, and mean MRC Scale of patients was improved from
. But in both cases, the variation was not statistically significant. After 30 days, mean ALSFRS-R score and mean MRC Scale of patients was more improved in subjects with bulbar-onset, onset age: 51-60yrs., disease duration: 24-48mo. And the results showed partially significant difference. Conclusions : We think that the results of this case be a pilot study that proves the effect of Oriental Medical treatment on ALS.
The Effect of the Uncariae Ramulus et Uncus on the Regeneration Following CNS Injury
Lee, Jin-Goo ; Park, Hyoung-Jin ; Kim, Dong-Woong ; Song, Bong-Keun ;
Journal of Pharmacopuncture, volume 12, issue 1, 2009, Pages 67~76
DOI : 10.3831/KPI.2009.12.1.067
Objective : Following central nervous system(CNS) injury, inhibitory influences at the site of axonal damage occur. Glial cells become reactive and form a glial scar, gliosis. Also myelin debris such as MAG inhibits axonal regeneration. Astrocyte-rich gliosis relates with up-regulation of GFAP and CD81, and eventually becomes physical and mechanical barrier to axonal regeneration. MAG is one of several endogenous axon regeneration inhibitors that limit recovery from CNS injury and disease. It was reported that molecules that block such inhibitors enhanced axon regeneration and functional recovery. Recently it was reported that treatment with anti-CD81 antibodies enhanced functional recovery in the rat with spinal cord injury. So in this current study, the author investigated the effect of the water extract of Uncariae Ramulus et Uncus on the regulation of CD81, GFAP and MAG that increase when gliosis occurs. Methods : MTT assay was performed to examine cell viability, and cell-based ELISA, western blot and PCR were used to detect the expression of CD81, GFAP and MAG. Then also immunohistochemistry was performed to confirm in vivo. Results : Water extract of Uncariae Ramulus et Uncus showed relatively high cell viability at the concentration of 0.05%, 0.1% and 0.5%. The expression of CD81, GFAP and MAG in astrocytes was decreased after the administration of Uncariae Ramulus et Uncus water extract. These results was confirmed in the brain sections following cortical stab injury by immunohistochemistry. Conclusion : The authors observed that Uncariae Ramulus et Uncus significantly down-regulates the expression of CD81, GFAP and MAG. These results suggest that Uncariae Ramulus et Uncus can be a candidate to regenerate CNS injury.
The importance of clinical mistletoe cancer therapy and korean mistletoe pharmacopuncture preparation development and application possibility for oriental medicine
Choi, Ok-Byung ;
Journal of Pharmacopuncture, volume 12, issue 1, 2009, Pages 77~89
DOI : 10.3831/KPI.2009.12.1.077
Objectives : Mistletoe extracts have been in use for around 85 years, predominantly in the area of cancer therapy. Today mistletoe preparations are among the most prescribed drugs in cancer medicine, thus constituting a standard biological therapy in the area of oncology. The purpose of this study is to analyze the practical implications of mistletoe cancer therapy, their clinical status, their preparation techniques and companies. Contents : Mistletoe therapy for cancer has been developed within the context of anthroposophical medicine. One major effect of mistletoe extract is that it stimulates the immune system and cancer defences. In Germany, a total of eight different mistletoe preparations are available, five developed by Anthroposophic Medicine and three evolved from research in phytotherapy. Therapy always consists of an introductory phase in order to test the patient`s tolerance, find the right dosage and choose the most suitable preparation. This paper covers the background of mistletoe medical plant materials, mistletoe therapy for cancer, the anthroposophical medicine and clinical research, the practical regulation of treatment, preparation of mistletoe drugs. Result & suggestion : Mistletoe extracts are a complementary teratment of cancer, widely used in intergrative cancer care. The study of the integration of korean mistletoe extracts to oriental cancer medicine, its development and feasibility in Korea are urgently needed. The products, substances, compositions of european mistletoe drugs are very similar to those of oriental medicine theory. Applying the mistletoe cancer therapy and its preparation techniques to oriental medicine, the herbal acupuncture preparation should be modernized and korean mistletoe products are to be developed. To this end, government and herbal acupuncture society need to interact each other for the development of oriental mistletoe cancer medicine.
Case Report of Three Cases of Idiopathic Oculomotor Nerve Palsy Treated with Hominis Placenta Pharmacopuncture
Jung, Ki-Hun ; Kim, Min-Seok ; Hwang, Hee-Sang ; Jeon, Jae-Cheon ; Park, Jae-Yeon ; Lee, Tae-Ho ; Lee, Eun-Yong ; Roh, Jeong-Du ;
Journal of Pharmacopuncture, volume 12, issue 1, 2009, Pages 91~97
DOI : 10.3831/KPI.2009.12.1.091
Objective : Oculomotor nerve palsy is a disorder which causes eyeball movement trouble, diplopia, dizziness and eyelid ptosis. It is caused by aneurysm, mass, trauma, infection and so on. But sometimes we can`t establish the cause. We observed 3 cases of idiopathic oculomotor nerve palsy patient treated with hominis placenta pharmacopuncture. Method & Result : We treated three idiopathic oculomotor nerve palsy patients with hominis placenta pharmacopuncture and electroacupuncture. As the result, symptoms of three patients were improved remarkably. Conclusion : In this cases, the hominis placenta pharmacopuncture is effective on idiopathic oculomotor nerve palsy. We need further study about idiopathic oculomotor nerve palsy and hominis placenta pharmacopuncture.
A case of foot drop treated with placenta pharmacopuncture
Jang, Woo-Seok ;
Journal of Pharmacopuncture, volume 12, issue 1, 2009, Pages 99~102
DOI : 10.3831/KPI.2009.12.1.099
This study was to evaluate a case of foot drop treated with placenta pharmacopuncure. After thirteen times of treatment, I evaluated this case by the angle of dorsiflexion, the total sum of gaiting points, the time of heel standing. As a result of evaluation, the angle of dorsiflexion, total sum of gaiting points, time of heel standing were improved. The placenta pharmacopuncture can be used for foot drop and other peripheral nerve palsy.
A Case Report of Carpal Tunnel Syndrome with Raynaud`s Phenomenon Treated by Bee Venom and Carthami Flos Pharmacopuncture
Choi, Seok-Woo ; Park, Pyeong-Beom ; Oh, Sung-Jong ;
Journal of Pharmacopuncture, volume 12, issue 1, 2009, Pages 103~108
DOI : 10.3831/KPI.2009.12.1.103
Objectives : This study is to report the effect of Pharmacopuncture therapy on a patient suffering from the pain and cold intolerance of hand caused by Carpal tunnel syndrome with Raynaud`s phenomenon. Methods : We had treated the patient diagnosed as Carpal tunnel syndrome with Raynaud`s phenomenon by Sweet BV and CF pharmacopuncture. We injected Sweet BV and CF into acupuncture points on both hands - Sweet BV into Baxie (EX-UE9), CF into Naegwan (
) and Daereung (
). And then we evaluated her symptoms by VAS (Visual Analog Scale). Results : Clinical symptoms about Carpal tunnel syndrome with Raynaud`s phenomenon were remarkably improved by Sweet BV and CF Pharmacopuncture. Conclusion : Therefore, we concluded that pharmacopuncture therapy - Sweet BV, CF etc. - may be useful to treat Carpal tunnel syndrome with Raynaud`s phenomenon.
Case Study of Oriental Medicine Treatment with acupotomy Therpy of the Tarsal tunnel Syndrome
Lim, Na-Ra ; Jang, Eun-Ha ; Park, Man-Young ; Kim, Sung-Chul ;
Journal of Pharmacopuncture, volume 12, issue 1, 2009, Pages 109~117
DOI : 10.3831/KPI.2009.12.1.109
Purpose : In order to estimate clinical effects of Oriental Medicine Treatment with acupotomy therapy of Tarsal tunnel Syndrome Methods : From 5th November, 2008 to 8th November, 2008, 1 male patient diagnosed as Tarsal tunnel syndrome(clinical diagnosed) was treated with general oriental medicine therapy (acupuncture, moxibustion, cupping, physical therapy, herbal medication) and acupotomy. Results : The patient`s Rt foot paresthesia, pain were remarkably improved. Conclusions : This study demonstrates that oriental medical treatment with acuputomy therapy has notable effect in improving symptoms of tarsal tunnel syndrome. as though we had not wide experience in this treatment, more research is needed.