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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Pharmacopuncture
Journal Basic Information
Journal DOI :
KOREAN PHARMACOPUNCTURE INSTITUTE
Editor in Chief :
Volume & Issues
Volume 14, Issue 4 - Dec 2011
Volume 14, Issue 3 - Sep 2011
Volume 14, Issue 2 - Jun 2011
Volume 14, Issue 1 - Mar 2011
Selecting the target year
Study of four weeks repeated-dose toxic test of Sweet Bee Venom in rats Original Articles
Kwon, Hae-Yon ; Kwon, Ki-Rok ;
Journal of Pharmacopuncture, volume 14, issue 1, 2011, Pages 5~24
DOI : 10.3831/KPI.2011.14.1.005
Objective: This study was performed to analyse four weeks repeated -dose toxicity of Sweet Bee Venom (SBV-pure melittin, the major component of honey bee venom) in rats. Methods: All experiments were conducted under the regulations of Good Laboratory Practice (GLP) at Biotoxtech Company, a non-clinical study authorized institution. Male and female rats of 5 weeks old were chosen for the pilot study of four weeks repeated-dose toxicity and was injected at the level of 0.56 mg/kg body weight (eighty times higher than the clinical application dosage as the high dosage), followed by 0.28 and 0.14 mg/kg as midium and low dosage, respectively. Equal amount of normal saline was injected as the control group every day for four weeks. Results: 1. No mortality was witnessed in all of the experiment groups. 2. All experiment groups appealed pain sense in the treating time compared to the control group, and side effects such as hyperemia and movement disorder were observed around the area of injection in all experiment groups, and the higher dosage in treatment, the higher occurrence in side effects. 3. Concerning weight measurement, neither male nor female groups showed significant changes compared to the control group. 4. Concerning to the CBC and biochemistry, all experiment groups didn`t show any significant changes compared to the control group. 5. Concerning weight measurement of organs, experiment groups didn`t show any significant changes compared to the control group. 6. To verify abnormalities of organs and tissues, those such as cerebellum, cerebrum, liver, lung, kidney, and spinal cords were removed and we conducted histologocal observation with H-E staining. Concerning the histologocal observation of liver tissues, some fatty changes were observed around portal vein in 0.56 mg/kg experiment group. But another organs were not detected in any abnormalities. 7. The proper high dosage of SBV for the thirteen weeks repeated test in rats may be 0.28 mg/kg in one time. Conclusion: Above findings suggest that SBV is relatively safe treatment medium. Further studies on the subject should be conducted to yield more concrete evidences.
The Study on toxicity and biological activities of Aconiti ciliare tuber Pharmacopuncture in Rats Original Articles
Kim, Sung-Chul ; Ahn, Seong-Hun ; Kim, Sung-Ha ; Lee, Sang-Kwan ; Song, Bong-Keun ;
Journal of Pharmacopuncture, volume 14, issue 1, 2011, Pages 25~33
DOI : 10.3831/KPI.2011.14.1.025
Objective : We investigate toxicity of Aconiti ciliare tuber and antioxidant activity of Aconiti ciliare tuber Pharmacopuncture to develop safe Aconiti ciliare tuber Pharmacopuncture and find out the effect. Methods : In order to investigate toxicity of Aconiti ciliare tuberm, we administered Aconiti ciliare tuberm orally to rats and examined the survival rate, comparing with the survival rate of rats administered by Radix aconitum simmered with Semen Glycine and Radix Glycyrrhizae. We examined the in vitro biological activity of Aconiti ciliare tuber Pharmacopuncture, including the total polyphenol content, and ABTS radical scavenging. Results and Conclusions : The
of Radix aconitum simmered with Semen Glycine and Radix Glycyrrhizae was 9.0g/kg, on the other hand, the
of Aconiti ciliare tuberm was more than 15g/kg. The total polyphenol contents of Aconiti ciliare tuberm Pharmacopuncture was 2.31mg/L. The 2,2`-azinobis-3-ehtlbezothiazoline-6-sulfonic acid radical decolorization (ABTS) was 10.26%. We conclude that Aconiti ciliare tuberm is not highly toxic, Aconiti ciliare tuber Pharmacopuncture has a little antioxidant effect.
Effect of pulse-wave factors in Middle Aged Women by Mountain Cultivated Ginseng Pharmacopuncture Original Articles
Park, Sang-Wook ; Kim, Yi-Soon ; Hwang, Won-Deok ; Kim, Gyeong-Cheol ;
Journal of Pharmacopuncture, volume 14, issue 1, 2011, Pages 35~49
DOI : 10.3831/KPI.2011.14.1.035
Objectives: The aim of this experiment is to know about Effect of pulse-wave factors in Middle Aged Women by Mountain Cultivated Ginseng Pharmacopuncture. Methods: First 20 Middle Aged women are diagnosed by pulse diagnosis, and then Mountain Cultivated Ginseng Pharmacopuncture(1 injection 20cc) were injected. 30 minutes later, pulse diagnosis again performed. As a result, method of one-group pretest-posttes design were used for evaluation. Results: T(Total pulse cycle) time statistically significant increased on both left and right chon, kwan, cheok. T4 time statistically significant increased on both left and right chon, kwan, cheok. T4-T1/T indexs except left cheokmaek, Right cheokmaek observation area decreased significantly in four sites. Wm(indicating high pressure retention time) indexs increased significantly in the five sites were observed except right chon maek. Conclusions: Effect of pulse-wave factors in Middle Aged Women by Mountain Cultivated Ginseng Pharmacopuncture increased T, T4, Wm and decreased T4-T1/T indexs. The results of this experiment, Mountain Cultivated Ginseng Pharmacopuncture induced to increase the Pulse-wave`s stability and strength.
Effects of Sipgeondaebo-tang Pharmacopuncture Extracts on the Collagenase Activity and Procollagen Synthesis in HS68 Human Fibroblasts and Tyrosinase Activity Original Articles
Lee, Se-Na ; Kim, Myung-Gyou ; Kim, Myoung-Hee ; Kim, Hyung-Jun ; Jo, Hak-Jun ; Leem, Kang-Hyun ;
Journal of Pharmacopuncture, volume 14, issue 1, 2011, Pages 51~59
DOI : 10.3831/KPI.2011.14.1.051
Objectives : This study was designed to investigate the collagen metabolism and tyrosinase activity of Sipgeondaebo-tang Pharmacopuncture extracts (SP). Methods : The effect of SP on type I procollagen production and collagenase activity in human normal fibroblasts HS68 after UVB (312 nm) irradiation was measured by ELISA method. The tyrosinase activity after treatment of SP was measured as well. Results : Type I procollagen production was recovered by SP in UVB damaged HS68 cells. The increased collagenase activity after UVB damage was significantly recovered by SP. The tyrosinase activity was significantly reduced as well. However, the L-DOPA oxidation was not changed. Conclusion : SP showed the anti-wrinkle effects and whitening effects in vitro. These results suggest that SP may be a potential pharmacopuncture as an anti-aging pharmacopuncture treatments.
Effects of Yukmigeehwang-hwan Extracts on the Elastase Activity and DPPH and NO Scavenging Activities Original Articles
Lee, Se-Na ; Kim, Myung-Gyou ; Kim, Myoung-Hee ; Kim, Hyung-Jun ; Jo, Hak-Jun ; Leem, Kang-Hyun ;
Journal of Pharmacopuncture, volume 14, issue 1, 2011, Pages 61~69
DOI : 10.3831/KPI.2011.14.1.061
Objectives : Elastic fibers are found in the skin, lungs, arteries, veins and other structures. Elastases destroy the elastic fibers and cause the emphysema and pulmonary hypertension. Oxidative stress is needed for these pathologic changes. Accordingly, present study was designed to investigate the effect of Yukmigeehwang-hwan extracts (YHE) on elastase activity and anti-oxidative effects of YHE. Methods : The inhibitory effects on elastase and DPPH and NO free radical scavenging activities of YHE were measured. Results : The elastase activity was significantly inhibited by YHE. YHE significantly scavenged DPPH and NO free radicals as well. Conclusion : YHE showed the elastase-inhibiting effects and anti-oxidative activities in vitro. These results suggest that YHE may have potential roles in the treatment of pulmonary emphysema and pulmonary hypertension.
The Comparative Study on Anti-inflammatory Antioxidant and Antibacterial Effects with Regard to the Extraction Solvents of Ligustici Rhizoma
HwangBo, Min ; Seo, Hyung-Sik ;
Journal of Pharmacopuncture, volume 14, issue 1, 2011, Pages 71~78
DOI : 10.3831/KPI.2011.14.1.071
Objective : This study was performed to compare anti-inflammation, anti-oxidation and anti-bacterial effects of Ligustici Rhizoma (LR) extracted with two kinds of solvents, ethanol and distilled water. Methods : It is prepared two kinds of LR extracts 20, 50, 100
by first. MTT assay way to measure cytotoxicity is formed in Raw 264.7 cell. The anti-inflammation effect is measured by ability to inhibit production of NO in Raw 264.7 cell. The anti-oxidation effect was measured by DPPH Radical scavenging ability in HaCaT cell. The anti-bacterial effect was measured by inhibition zone diameter on Propionibacterium acnes. Results : 1. LR (20
) extracted with ethanol was showed 80% cytotoxicity, LR (50
) extracted with ethanol and LR (20, 50
) extracted with water were showed 70% cytotoxicity, LR (100
) extracted with ethanol and LR (100
) extracted with water were showed 60% cytotoxicity in Raw 264.7 cell. 2. LR (100
) extracted with ethanol was showed more significantly inhibitory effect on NO production than the water extraction. 3. Two kinds of LR extraction groups did not show significantly scavenging effect of DPPH radicals. 4. Two kinds of LR extractions did not have a inhibitory effect on Propionibactrium acnes. Conclusion : Two kinds of LR extracts have not cytotoxicity, statistically significant ability to scavenge DPPH radicals and effect to inhibit Propionibactrium acnes. LR extracted with ethanol only have a little effect to inhibit NO production. This study proposes that LR extracted with ethanol is more effective in anti-inflammation.
The Comparative Study of Anti-inflammatory, Antioxidant and Antibacterial Effects with Regard to the Extraction Solvents of Cuscutae Semen
HwangBo, Min ; Seo, Hyung-Sik ;
Journal of Pharmacopuncture, volume 14, issue 1, 2011, Pages 79~86
DOI : 10.3831/KPI.2011.14.1.079
Objective : This study was performed to compare anti-inflammation, anti-oxidation and anti-bacterial effects of Cuscutae Semen(CS) extracted with two kinds of solvents, ethanol and distilled water. Methods : Two kinds of CS extractions were prepared 20, 50, 100
. The cytotoxicity was measured by MTT assay in Raw 264.7 cell. The anti-inflammation effect was measured by inhibitory efficacy of NO Production in Raw 264.7 cell. The anti-oxidation effect was measured by DPPH Radical scavenging ability in HaCaT cell. The anti-bacterial effect was measured by inhibition zone diameter on Propionibacterium acnes. Results : 1. Two kinds(100
) of CS extraction groups had 50% cytotoxicity in Raw 264.7 cell. 2. All of CS extraction groups were not showed significantly inhibitory effect on NO production. 3. All of CS extracted with ethanol only showed dose-dependently significantly scavenging effect of DPPH radicals. 4. Two kinds of CS extractions did not have a inhibitory effect on Propionibactrium acnes. Conclusion : Two kinds(100
) of CS extraction groups have 50% cytotoxicity. Two kinds of CS extractions have not the inhibitory effect on NO production and Propionibactrium acnes. CS groups extracted with ethanol only have a significantly scavenging ability of DPPH radicals. This study suggests that CS extracted with ethanol was effective in anti-oxidation.
Case Study of Oriental Medicine Treatment with Acupotomy Therapy of the Peroneal Nerve Palsy through Ultrasound Case Report
Kim, Sung-Ha ; Park, Man-Young ; Lee, Sang-Mi ; Lee, Sang-Kwan ; Lim, Jin-Young ; Kim, Sung-Chul ;
Journal of Pharmacopuncture, volume 14, issue 1, 2011, Pages 87~95
DOI : 10.3831/KPI.2011.14.1.087
Purpose : In order to estimate clinical effects of Oriental Medicine Treatment with acupotomy therapy of Peroneal nerve Palsy. Methods : From 10th June, 2010 to 19th June, 2010, 1 female patient diagnosed as Peroneal nerve Palsy(clinical diagnosed) was treated with general oriental medicine therapy (acupuncture, pharmacopuncture, moxibustion, cupping, physical therapy, herbal medication) and acupotomy. Results : The patient`s left foot drop was remarkably improved. Conclusions : This study demonstrates that oriental medical treatment with acuputomy therapy has notable effect in improving symptoms of peroneal nerve palsy. as though we had not wide experience in this treatment, more research is needed.
The Clinical Study on a case of Fibromyalgia Syndrome(FMS) Patient Case Report
Lee, Yoo-Hwan ; Kim, Jung-Hee ; Lee, Chang-Hwan ; Kim, Cheol-Hong ; Youn, Hyoun-Min ;
Journal of Pharmacopuncture, volume 14, issue 1, 2011, Pages 97~104
DOI : 10.3831/KPI.2011.14.1.097
Objective : This study is to report the effect that taken by using oriental treatment on the patient with Fibromyalgia Syndrome(FMS). Methods : We have observed this case of patient treated by Acupuncture therapy, pharmacopuncture therapy and herbal medication. Results : There was improvement in condition of the patient who had Fibromyalgia Syndrome(FMS) after treated by using oriental treatment. Conclusion : Oriental treatments such as Acupuncture therapy, Pharmacopuncture therapy and herbal medication can be effective for the Fibromyalgia Syndrome(FMS).