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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Animal Science and Technology
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Journal DOI :
Korean Society of Animal Science and Technology
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Volume & Issues
Volume 50, Issue 6 - Dec 2008
Volume 50, Issue 5 - Oct 2008
Volume 50, Issue 4 - Aug 2008
Volume 50, Issue 3 - Jun 2008
Volume 50, Issue 2 - Apr 2008
Volume 50, Issue 1 - Feb 2008
Selecting the target year
Genetic Variation and Relationships of Korean Cattle(Hanwoo) and Foreign Breeds Using Microsatellite Markers
Oh, Jae-Don ; Kong, Hong-Sik ; Lee, Jae-Hyeon ; Yang, Dae-Yong ; Jeon, Gwang-Joo ; Lee, Hak-Kyu ;
Journal of Animal Science and Technology, volume 50, issue 6, 2008, Pages 733~740
DOI : 10.5187/JAST.2008.50.6.733
The purpose of this study was to assess the genetic variation and establish the relationship amongst Hanwoo, Angus and Holstein breed. The genetic characteristics and variability within Hanwoo(300), Angus(80) and Holstein(50) were estimated on the basis of relationships determined using the 10 kinds of microsatellite, which is located on different chromosomes. Frequencies of microsatellites markers were used to estimate heterozygosities, polymorphic information content(PIC) and genetic distances. The PICs ranged from 0.604 to 0.872(Hanwoo), from 0.562 to 0.812(Angus) and from 0.471 to 0.828(Holstein). Observed heterozygosity and PIC of Hanwoo are the highest among the analyzed breeds. Additionally, Estimates of genetic distance can be utilized to identify genetic relationships between Hanwoo and the other breed. Genetic distances(0.233) between Hanwoo and Angus was lower than distances between Hanwoo and Holstein(2.283). Also, Genetic distances between Angus and Holstein was shown for(2,400). The other side, each individuals were not ramified to different group and were spread evenly in phylogenetic dendrogram about all the populations.
Effects of Change of Body Weight on Racing Time in Thoroughbred Racehorses
Cho, Kwang-Hyun ; Son, Sam-Kyu ; Cho, Byung-Wook ; Lee, Hak-Kyo ; Kong, Hong-Sik ; Jeon, Gwang-Joo ; Park, Kyung-Do ;
Journal of Animal Science and Technology, volume 50, issue 6, 2008, Pages 741~746
DOI : 10.5187/JAST.2008.50.6.741
The objective of this study was to estimate the effects of change in body weight on racing time in Thoroughbred racehorses, using total 8,197 horses and 155,656 racing records collected from Korea Racing Association(KRA). The average body weight of the racehorses was 449kg, and the average body weights of stallion, gelding and mare were 460kg, 454kg and 441kg, respectively. Body weight of stallion was the greatest. The considerable loss of body weight was observed in March, April and May for mare, and in May, Jun and July for stallion and gelding. Overall, the body weight of the racehorses decreased in spring and summer, and increased in autumn and winter. The estimated heritability and repeatability for racing time were 0.237 and 0.525, respectively. The heritability for body weight estimated from total records was 0.612. The estimated heri- tabilities for body weight were 0.472, 0.578 and 0.555 for gelding, stallion and mare, respectively. As the change of body weight was greater than ±10kg, the racing time increased significantly. When the body weight changed more than ±20kg in comparison to the changes of body weight of ±5kg, the racing time increased by 0.3 second. When the change of body weight was ±0.5% of body weight, the racing time was the best(fastest). When the change of body weight was more than ±2.5%, racing performance decreased considerably.
Trends on Racing Speed Traits in Thoroughbred Racehorses
Park, Kyung-Do ; Lee, Hak-Kyo ; Jeon, Gwang-Joo ; Kong, Hong-Sik ; Cho, Byung-Wook ; Cho, Kwang-Hyun ; Son, Sam-Kyu ;
Journal of Animal Science and Technology, volume 50, issue 6, 2008, Pages 747~752
DOI : 10.5187/JAST.2008.50.6.747
This study was carried out to calculate genetic trends on racing speed traits of Thoroughbred racehorses, using a total 208,043 racing records of 9,934 heads collected from January, 1990 to December, 2006 in Gwacheon racecourse. Repeated time, winning time and annual best time were used racing speed traits. The estimated heritabilities and repeatabilities for repeated time, winning time and annual best time were 0.288, 0.275, 0.341 and 0.502, 0.475, 0.496, respectively. Average phenotypic improvement per race year for racing speed traits were ranged from －0.115 to 0.148 second. The other side, the genetic improvement per race year for repeated time was －0.027 second but winning time and annual best time were not shown consistent trends. Therefore, we concluded that repeated time is recommended improvement trait of Thoroughbred racehorses.
Cloning, cSNP Identification, and Genotyping of Pig Complement Factor B(CFB) Gene Located on the SLA Class III Region
Kim, Jae-Hwan ; Lim, Hyun-Tae ; Seo, Bo-Yeong ; Zhong, Tao ; Yoo, Chae-Kyoung ; Jung, Eun-Ji ; Jeon, Jin-Tae ;
Journal of Animal Science and Technology, volume 50, issue 6, 2008, Pages 753~762
DOI : 10.5187/JAST.2008.50.6.753
The primers for RT-PCR and RACE-PCR were designed by aligning the pig genomic sequence and the human complement factor B(CFB) coding sequence(CDS) from the GenBank. Each PCR product was amplified in pig cDNA and sequencing was carried out. The CDS length of pig CFB gene was determined to be 2298 bp. In addition, the pig CDS was more longer than human and mouse orthologs because of insertion and deletion. The identities of porcine nucleotide sequences with those of human and mice were 84% and 80%, and the identities of amino acids were 79% to 77%, respectively. Three complement control protein(CCP) domains, one Von Willebrand factor A(VWFA) domain and a serine protease domain, that are revealed typically in mammals, were found in the pig CFB gene. Based on the CDSs determined, the primers were designed in intron regions for amplification of entire length of exons. In amplification and direct sequencing with genomic DNAs of six pig breeds, three cSNPs(coding single nucleotide polymorphisms) were identified and verified as missense mutations. Using the Multiplex-ARMS method, we genotyped and verified the mutations identified from direct sequencing. To demonstrate recrudescence, we performed both direct sequencing and Multiplex-ARMS with two randomly selected DNA samples. The genotype of each sample exhibited the same results using both methods. Therefore, three cSNPs were identified from pig CFB gene and that can be used for haplotype analysis of the swine leukocyte antigen(SLA) class III region. Moreover, the results indicate that the Multiplex-ARMS method should be powerful for genotyping of genes in the SLA region.
The Effect of Body Measurements Type on Carcass Traits in Hanwoo
Sun, Du-Won ; Kim, Byeong-Woo ; Park, Jong-Won ; Moon, Won-Gon ; Choo, Hyo-Jun ; Jeon, Jin-Tae ; Lee, Jung-Gyu ;
Journal of Animal Science and Technology, volume 50, issue 6, 2008, Pages 763~774
DOI : 10.5187/JAST.2008.50.6.763
This study was carried out to investigate the correlation of body measurement type and carcass traits. This study used body measurement type and carcass traits on 1,312 heads of Hanwoo steers tested(24 months) which was from 1997 to 2002 at Hanwoo Improvement Main Center National Agricultural Cooperatives Federation. Body measurement type used the age of 18 months body measurement type, and carcass traits used the results of decision grade of Animal Products Grading Service. Observing the phenotypic correlation between carcass traits and body measurement type from the result, the weight, withers height, and chest width at age of 18 months and carcass weight showed readings of 0.690, 0.483, and 0.506, respectively. Also, eye muscle area and age of 18 months weight and chest width read 0.356 and 0.279, respectively. According to results, that improvements in weight, withers height, and chest width are likely to enlarge the eye muscle area and meat production level. Not only quality improvement but also quantity augmentation affect income and sales of the farmers. As can be seen in this study, improvements of weight, withers height and chest width should always be put to much effort while other body measurement type should be considered continuously too. In order to attain clearer results further studies should be done on body measurement type, which will contribute to the improvements of body measurement type in Hanwoo.
Discovery of Candidate SNP Related to Meat Quality Using the BcSNPdb and Cattle QTLdb in Hanwoo
Kim, Dae-Hyun ; Lee, Yoon-Seok ; Oh, Young-Sook ; Choi, Chang-Bon ; Yeo, Jung-Sou ;
Journal of Animal Science and Technology, volume 50, issue 6, 2008, Pages 775~782
DOI : 10.5187/JAST.2008.50.6.775
This study was aimed to find SNPs related with marbling score in Hanwoo(Korean cattle) using BcSNPdb. This study was searched QTL region related with marbling score and extracted 3,605 SNPs by applying BcSNPdb. Among these SNPs, 347 nonsynonymous SNP were selected and 160 SNPs were verified by PCR and finally proven with application to experimental data of the national progeny test. BTS_003888, BTS_012665 and BTS_009454 candidate SNPs were revealed significantly associated with marbling score(P<0.05), and BTS_025951 candidate SNPs was significantly associated with backfat thickness(P<0.05). From the result, SNPs from BTS_003888, BTS_009454, BTS_052584 and BTS_025951 were considered to be useful for the advancement in selective improved model in marbling score and backfat thickness of Hanwoo.
Analysis of Differential Expression of Cytochrome P450 Aromatase(Cyp19) in The Efferent Ductules and The Epididymis of Male Rats During Postnatal Development
Kim, Ju-Young ; Seo, Hee-Jung ; Kim, Ok-Soon ; Kim, Byung-Joon ; Lee, Seong-Kyu ; Baik, Haing-Woon ; Lee, Ki-Ho ;
Journal of Animal Science and Technology, volume 50, issue 6, 2008, Pages 783~792
DOI : 10.5187/JAST.2008.50.6.783
The present study was performed to determine expression of cytochrome P450 aromatase(Cyp19) in the efferent ductules(EDs) and the epididymis of male rat reproductive tract at different postnatal ages. Total RNAs isolated were reverse-transcribed, and cDNAs were utilized for real-time PCR analysis. In the EDs, the Cyp19 transcript was expressed at all prepubertal ages with the highest level at 14 days of age, but not at 90 days of age. Expression of Cyp19 mRNA in the epididymis was found at all age groups, except 7 days of age. Distinct expression patterns of Cyp19 transcript were shown in each segment of the epididymis. These results indicate that expression of Cyp19 gene in the excurrent duct of male reproductive tract is differentially regulated in age-dependent and segment-specific manners.
Characterization of Bacteria and Their Antibiotic Sensitivities in Porcine Liquid Semen
Ryu, Jae-Weon ; Cho, Kyu-Ho ; Hong, Joon-Ki ; Kim, Myung-Jick ; Park, Jun-Chul ; Jung, Il-Byung ; Kim, In-Cheul ;
Journal of Animal Science and Technology, volume 50, issue 6, 2008, Pages 793~798
DOI : 10.5187/JAST.2008.50.6.793
The semen collection process in the porcine is far from being a sterile procedure. Consequently, porcine ejaculates commonly contain bacterial contaminants. The aim of this study is to identify the bacteria in porcine semen and to find the antibiotics resistance of bacteria. Twelve porcine originating from four AI center were used to collect semen. Bacteria were identified by automated instrument for rapid organism identification system and bacterial sensitivities of 8 antibiotics were tested. The Bacterial contaminants of Staphylococcus genus(37.8%), Proteus genus(7.0%), Bacillus genus (6.1%), Pasteulla genus(5.7%), Acinetobacte genus(5.2%), Serratia genus(4.3%) and others(33.9%) were frequently isolated. However, amikacin showed higher antibiotic sensitivity than other antibiotics. General sanitation protocols can contribute partly to inhibit the bacterial contamination, with monitoring boar housing, semen collection areas and the extended semen. But, proper selection of preservative antibiotics by microbial sensitivities can minimize the influence of bacteria.
Effects of Feeding High and Low Ca Additive on Laying Performance and Egg Quality in Laying Hens
Kang, Hwan-Ku ; Kang, Geun-Ho ; Kim, Dong-Wook ; Na, Jae-Cheon ; Yu, Dong-Jo ; Lee, Sang-Jin ; Kim, Sang-Ho ;
Journal of Animal Science and Technology, volume 50, issue 6, 2008, Pages 799~806
DOI : 10.5187/JAST.2008.50.6.799
This experiment was carried out to investigate the effect of feeding limestone to high and low Ca layer diets on laying performance and eggshell quality in laying hens. Five hundred and twenty-eight HyLine brown layer allotted to six treatments consisting of high and low Ca diets. Limestone added to two kinds of basal diet with 1, 2 and 3 fold compared to conventional diet. Egg production was not different regardless Ca level and amount of added limestone in overall periods. However, the increment of egg observed in high Ca diet and added 10g limestone group during later period. Ca and limestone intake were increased as increasing feeding limestone. Eggshell strength and thickness were improved in high Ca consumption group. It was concluded that feeding Ca sources to conventional diets was helpful improving eggshell quality and reducing feed cost per egg mass by increasing proportion of Ca intake.
Study on Intestinal Viability and Optimum Feeding Method of Lactobacillus in Broiler Chickens
Kim, Dong-Wook ; Kim, Ji-Hyuk ; Kang, Geun-Ho ; Kang, Hwan-Ku ; Lee, Sang-Jin ; Lee, Won-Jun ; Kim, Sang-Ho ;
Journal of Animal Science and Technology, volume 50, issue 6, 2008, Pages 807~818
DOI : 10.5187/JAST.2008.50.6.807
This study was conducted to prove the optimum feeding method of Lactobacillus in broiler chickens by investigating the intestinal viability of ingested Lactobacillus and the effect of feeding levels and frequency of Lactobacillus on growth performance in broiler chickens. In experiment 1, A total of one hundred, 5 weeks old male broiler chickens(Abor Acre) were fed Lactobacillus reuteri avibro2 expressed green fluorescent protein(GFP) at 104cfu/g diet to investigate the retention time of ingested Lactobacillus in the intestine for 1 day. The percentage of Lactobacillus expressed GFP in intestinal contents was 26% at 1 day after fed Lactobacillus expressed GFP. The percentage of Lactobacillus expressed GFP in intestinal contents was decreased in length of time. In experiment 2, A total of four hundred eighty, 1-d-old male broiler chicks(Abor Acre) were randomly divided into 4 groups with 4 replicates of 30 birds each to prove the optimum feeding level of Lactobacillus. The treatments were control(free antibiotics), Lactobacillus reuteri avibro2 5.0×10cfu/mL, 5.0×103cfu/mL, and 5.0×105cfu/mL. The final body weight and body wight gain of Lactobacillus reuteri avibro2 5.0×103cfu/mL were the highest in all groups(P<0.05). Feed conversion ratio was not significantly difference among the groups. The number of intestinal lactic acid bacteria in Lactobacillus treated groups tended to be improved or significantly increased as compared to that of control(P<0.05). Protein and fat digestibility in Lactobacillus 5.0×103cfu/mL and 5.0×105cfu/mL treated groups were significantly improved(P<0.05). No significant differences were observed on the availability of dry matter and crude ash in Lactobacillus treatments compared to those of control. In experiment 3, A total of six hundred 1-d-old male broiler chicks(Abor Acre) were randomly divided into 4 groups with 4 replicates of 30 birds each and were fed Lactobacillus reuteri avibro2 at intervals of 1, 2, 3, and 5 day for five weeks. Feeding level of Lactobacillus was 5.0×103cfu/mL The final body weight and body wight gain of Lactobacillus reuteri avibro2 5.0×103cfu/mL were the highest in all groups(P<0.05). The final body weight and body weight gain were significantly increased, when Lactobacillus was fed at intervals of 1 days, or 2 days. There were no significant differences in feed intake and feed conversion ratio among the all groups. The number of intestinal lactic acid bacteria in Lactobacillus treated groups tended to be improved or significantly increased as compared to that of control(P<0.05). No significant differences were observed on the number of coliform bacteria and Salmonella of ileum and cecum. Consequently, supplemental Lactobacillus influenced positive effects on the growth performance, nutrient availability and intestinal microflora. The optimum feeding level of Lactobacillus was 5.0×103cfu/mL, and the constant feeding of Lactobacillus was effective.
Evaluation of Extruded Rice Product as Alternative to Lactose on Growth Performance, Diarrhea Score, Fecal E.coli and VFA in Weaning Pigs
Kim, Kwang-Ho ; Choi, Young-Hwan ; Long, Hong-Feng ; Ju, Won-Seok ; Son, Seong-Hoon ; Oh, Hee-Kyung ; Kim, Yoo-Yong ;
Journal of Animal Science and Technology, volume 50, issue 6, 2008, Pages 819~830
DOI : 10.5187/JAST.2008.50.6.819
The object of this study was to investigate different levels of extruded rice product(Nutri-RS®) supplementation as an alternative to lactose on growth performance, diarrhea score, fecal E.coli and volatile fatty acid(VFA) in weaning pigs. Treatments were 1) Control(basal), L3(basal+3% extruded rice product), L6(basal+6% extruded rice product). The control diet in Phase I and Phase II contained 16 and 9% of lactose, respectively. For the feeding trial, a total of 81 crossbred pigs, averaging 7.56±1.18 kg body weight, were allotted to three treatments in three replicates with nine pigs per pen by RCBD. The experimental period was total 5 weeks. During the whole experimental period, there were no significant differences in growth performance, diarrhea score, fecal volatile fatty acid concentration among all treatments. Although there were no significant differences, pigs fed diet of L6 tended to show higher BW and ADG, ADFI, and fecal volatile fatty acid concentration compared to other treatments. Treatment L6 showed the lowest fecal E. coli count among all treatments(P<0.05). These results suggested that inclusion of extruded rice product has similar performance a with 6% of lactose in diet for weaning pigs and reduce approximately 9% production cost of weaning pig's diet. Therefore, extruded rice product could be utilized as an alternative feed ingredient to lactose in weaning pig’s diet.
Effect of Mixed Microbes Addition on Chemical Change and Silage Storage of Spent Mushroom Substrates
Kim, Young-Il ; Seok, Joon-Sang ; Kwak, Wan-Sup ;
Journal of Animal Science and Technology, volume 50, issue 6, 2008, Pages 831~838
DOI : 10.5187/JAST.2008.50.6.831
This study was conducted to evaluate effects of mixed microbes addition on physico-chemical, fermentative and microbial parameters of sawdust-based spent mushroom substrates(SMS). The SMS was inoculated with mixed microbes(Enterobacter ludwigii, Bacillus cereus, 2 strains of Bacillus subtillis, Saccharomyces cerevisiae and Lactobacillus plantarum) at 1% level(wet basis) and anaerobically fermented during the different periods(up to 8th week). Compared with the SMS before ensiling, the ensiled one had higher CP, NDF and ADF percentages and lower DM and NFC percentages. However, levels of change were very low. The in situ ruminal disappearance of SMS DM and NDF decreased with the ensiling period prolonged. For fermentative parameters, pH reduced and lactic acid contents increased after ensiling, compared with those after ensiling. At 8th week of ensiling, pH increased and lactic acid contents reduced again, compared with those at 4th week of ensiling; however, the silage still showed favorable fermentation status. Lactic acid bacteria counts did not change throughout 8 weeks of ensiling. Counts of total microbes and yeast reduced after 4th week of ensiling period. Counts of lactic acid bacteria and yeast at 8th week of ensiling were in the levels of 108cfu/g. These results indicate that anaerobic fermentation with microbial addition could be an effective way for the long term(8 weeks) storage of the SMS.
Effects of Dietary Mugwort Powder on Carcass and Meat Quality Characteristics of Gilt and Barrow in Growing-Finishing Period
Kim, Il-Suk ; Jin, Sang-Keun ; Song, Young-Min ; Kim, Chul-Wook ; Jo, Kwang-Keun ; Chung, Ki-Hwa ; Kang, Suk-Nam ;
Journal of Animal Science and Technology, volume 50, issue 6, 2008, Pages 839~848
DOI : 10.5187/JAST.2008.50.6.839
This study was conducted to determine the effect of mugwort powder on carcass characteristics, proximate composition, cholesterol contents, and meat qualities in growing-finishing gilt and barrow(LY×D). Totally 80 pigs (40 gilts and 40 barrows, approximately 50kg each) with 10 pigs per treatment were randomly alloted. The diet group was prepared as 0(C), 0.5(T1), 1.0(T2), and 1.5%(T3) mugwort powder in basal diet, respectively. They were fed experimental diets for 90 days before slaughtered. Carcass weight was increased by dietary mugwort powder(p<0.05), whereas changes in back fat thickness and meat grade were unaffected by dietary supplements. Meat moisture, fat, ash, and cholesterol were not differ significantly in the samples, however, protein in T2 and T3 were significantly higher than the control (p<0.05). Meat pH48, water holding capacity, and cooking loss were not differ significantly in the samples (p>0.05), and shear force of fresh meat and fat were not differ significantly in the samples(p>0.05). Lightness(L*) of meat was increased but that of fat was decreased by dietary mugwort powder, whereas changes in redness(a*) and yellowness(b*) were unaffected by dietary supplements.
cDNA Microarray Analysis of Gene Expression in Pig Spleen Lymphocytes in Response to Extract of Raspberry
Chung, Chung-Soo ; Choi, Young-Sook ; Lim, Hee-Kyong ; O, Yun-Genel ; Mandal, Prabhat Kumar ; Choi, Kang-Duk ;
Journal of Animal Science and Technology, volume 50, issue 6, 2008, Pages 849~856
DOI : 10.5187/JAST.2008.50.6.849
The present study was undertaken to investigate specific immune response of Rubus coreanus Miquel (raspberry) in pig spleen lymphocytes and gene expression induced by the extracts of raspberry using gene chip technology. The 70% ethyl alcohol extracts of raspberry were treated to pig spleen lymphocytes. The extracts of raspberry stimulated the proliferation of splenocytes and increased the population of CD3 & CD4 T-cells and B-cells in pig spleen lymphocytes. The extracts of raspberry improved immune response by increasing the viability of splenocytes. In microarray study we found eight genes were significantly up- regulated by the extracts of raspberry in pig splenocytes, including genes known to be involved in cell structure and immune response, particularly microtubule-associated protein 4, cytoplasmic dynein heavy chain, tumor necrosis factor alpha, lymphotoxin-beta receptor precursor. However, ten genes were down- regulated by the extracts of raspberry treatment.
A Survey on the Stable Vice of Race Horse in Stable
Yoon, Sei-Young ; Kim, Myeong-Hwa ; Lee, Sang-Rak ;
Journal of Animal Science and Technology, volume 50, issue 6, 2008, Pages 857~864
DOI : 10.5187/JAST.2008.50.6.857
This study was conducted to identify various habits of stable vices and its occurring frequency in racing horses, currently managed in Korea, through categorizing the total 1,386 head of racing horses in Seoul Racing Park into sex, age and years of stabling. Among the 1,386 horses, 524 heads(37.8%) have shown habits of stable vices and its appearances rate according to each category is as following; While 40.7% of stallions showed the highest appearances rate of stable vices by sex, it was 50%, 44.2%, and 53% for the age of 2, 6 and 4, respectively by age, it was 41%, 40.6% and 39.1% for the years of stabling of 2, 4 and 3, respectively. For the appearances rate of individual stable vices, 7.0% of mares showed the highest appearances rate of ‘kicking at walls’, 12.9% of stallions showed ‘biting habit’ and 5.7% of castrated horses showed ‘weaving’ when grouped by sex. According to age, 8.3%, and 6.1% of horses of age 2 showed ‘kicking at walls’, ‘wind-sucking’ and ‘pawing’, respectively while 4.9% of horses of age 3 showed ‘pawing’, 6.4% of horses of age 4 showed ‘kicking at walls’, 6.8% of horses of age 5 showed ‘kicking at walls’ and ‘biting habit’ and 8.7% of horses of age 6 showed ‘weaving’ and ‘biting habit’. By the year of stabling, 6% of horses for 1 year had ‘pawing habit’, 7% og horses for 2 years had ‘kicking at walls’, 7.4% of horses for 3 years had ‘biting habit’ and it was 6.9% and 10.6% for ‘weaving’ for 4 and 5 years of stabling, respectively. In conclusion, the racing horses in Korea seemed to show high rate of appearance rate of stable vices and it is considered to be necessary to improve stable equipments and management skills to reduce the appearance rate of stable vices.