Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Animal Science and Technology
Journal Basic Information
Journal DOI :
Korean Society of Animal Science and Technology
Editor in Chief :
Volume & Issues
Volume 51, Issue 6 - Dec 2009
Volume 51, Issue 5 - Oct 2009
Volume 51, Issue 4 - Aug 2009
Volume 51, Issue 3 - Jun 2009
Volume 51, Issue 2 - Apr 2009
Volume 51, Issue 1 - Feb 2009
Selecting the target year
Estimation of Genetic Parameter for Linear Type Traits in Holstein Dairy Cattle in Korea
Lee, Ki-Hwan ; Sang, Byung-Chan ; Nam, Myoung-Soo ; Do, Chang-Hee ; Choi, Jae-Gwan ; Cho, Kawng-Hyun ;
Journal of Animal Science and Technology, volume 51, issue 5, 2009, Pages 345~352
DOI : 10.5187/JAST.2009.51.5.345
This study utilized 332,625 records of linear type scores consisting for 15 primary traits, 22,175 final score and 84,612 pedigree information of 22,175 Holstein cows from 1993 to 2007 in Korea to estimate genetic parameters for 16 type traits. Genetic and error (co)variances between two traits selected from 16 traits were estimated using bi-trait pairwise analyses with DFREML package. The estimated heritabilities for stature (ST), strength (STR), body depth (BD), dairy form (DF), rump angle (RA), thurl width (TW), rear legs side view (RLSV), foot angle (FA), fore udder attachment (FUA), rear udder height (RUH), rear udder width (RUW), udder cleft (UC), udder depth (UD), front teat placement (FTP), front teat length (FTL) and final score (FS) were 0.31, 0.21, 0.25, 0.10, 0.29, 0.19, 0.09, 0.06, 0.12, 0.13, 0.12, 0.08, 0.26, 0.20, 0.28 and 0.15, respectively. ST had the highest positive genetic correlation with BD (0.90), while RLSV had the highest negative genetic correlation with FA (-0.56). RA had negative genetic correlation with most udder traits (-0.17~-0.02). Especially, RUW had the higher positive genetic correlation with STR (0.60), BD (0.62), and TW (0.49), however, UD had the higher negative genetic correlation with STR (-0.40) and BD (-0.40). FTL had negative genetic correlation with FUA, RUH, RUW, UC and UD. FS had positive genetic correlation with UC, UD and FTP (0.12, 0.18 and 0.20). However, additional research is needed on the use of these parameters in the genetic evaluation because estimated genetic and error variance-covariance matrices were not positive definite.
A Comparison of Discriminating Powers Between 14 Microsatellite markers and 60 SNP Markers Applicable to the Cattle Identification Test
Lim, Hyun-Tae ; Seo, Bo-Yeong ; Jung, Eun-Ji ; Yoo, Chae-Kyoung ; Yoon, Du-Hak ; Jeon, Jin-Tae ;
Journal of Animal Science and Technology, volume 51, issue 5, 2009, Pages 353~360
DOI : 10.5187/JAST.2009.51.5.353
When 14 microsatellite (MS) markers were applied in the identifying test for 480 Hanwoo, the discriminating power was estimated as
based on the assumption of a random mating group (PI). This rate is 1,000 times higher than that of 60 single nucleotide polymorphism (SNP) markers. On the other hand, the power of the 60 SNP markers was estimated as
on the assumption of a half-sib mating group (
) and a full-sib mating group (
), respectively. These powers were 10 times and 10,000 times higher than those of the 14 MS markers. The results indicated that the total number of alleles (MS vs SNP = 146 vs 120) acted as a key factor for the discriminating power in a random mating population, and the total number of markers (MS vs SNP = 14 vs 60) was a dominant influence on the power in half-sib and full-sib populations. In the Hanwoo population, in which it was assumed that the entire population is the enormous half-sib group formed by the absolute genetic contribution of a few nuclear bulls, there will be only a 10 times difference in the discriminating power between the 14 MS markers and the 60 SNP makers. However, the probability of not excluding a candidate parent pair from the parentage of an arbitrary offspring, given that only the genotype of the offspring (
) was 1,000 times higher as shown by the 14 MS markers than that by the 60 SNP markers. The strong points of SNP makers are the stability of the variation (low mutation rate) and automation of high-throughput genotyping. In order to apply these merits for the practical and constant Hanwoo identity test, research and development are required to set a cost-effective platform and produce a homemade apparatus for SNP genotyping.
Karyotype of Jeju Horse; G-, C- and NOR-banding
Park, Jin-Sik ; Cho, Byung-Wook ; Sohn, Sea-Hwan ;
Journal of Animal Science and Technology, volume 51, issue 5, 2009, Pages 361~368
DOI : 10.5187/JAST.2009.51.5.361
This study was carried out to establish the standard karyotype of Jeju horse by G-, C- and AgNOR-banding patterns. Blood samples were collected from 37 Jeju horses and 24 Thoroughbred that had been raised at the National Institute of Subtropical Agriculture in Jeju. The lymphocytes were cultured in vitro and then chromosomes prepared. The diploid chromosome number of Jeju horse is 64, which consists of 31 pairs of autosomes and X, Y sex chromosomes. The Jeju horse has 13 pairs of metacentric/submetacentric and 18 pairs of acrocentric autosomes. The X chromosome is the fifth largest submetacentric, while the Y chromosome is one of the smallest acrocentric chromosomes. The G-banding pattern of Jeju horse chromosomes showed a light band at centromeres in all autosomes, and also exhibited a typical and identical banding pattern in each homologous chromosome. Overall chromosomal morphology and positions of typical landmarks of the Jeju horse were virtually identical to those of International Committee for the Standardization of the Domestic Horse Karyotype. C-bands of Jeju horse chromosomes appeared on centromeres of almost all autosomes, but chromosome 8 showed a heterochromatin heteromorphism. The NORs in Jeju horse chromosomes showed polymorphic patterns within breed, individuals and cells. By the AgNOR staining, the NORs were located at the terminal of p-arm on chromosome 1 and near centromeres on the chromosome 26 and 31. The mean number of NORs per metaphase was 4.68 in Jeju horse.
Protein Fractionation of Whole Crop Silages, and Effect of Borate-phosphate Buffer Extraction on In vitro Fermentation Characteristics, Gas Production and Degradation
Shinekhuu, Judder ; Jin, Guang-Lin ; Ji, Byung-Ju ; Li, Xiangzi ; Oh, Young-Kyoon ; Hong, Seong-Ku ; Song, Man-Kang ;
Journal of Animal Science and Technology, volume 51, issue 5, 2009, Pages 369~378
DOI : 10.5187/JAST.2009.51.5.369
Protein fractionation was evaluated from whole crop silages of rye (RS), wheat (WS), triticale (TS), oat (OS), barley (BS), and rice straw silage (RSS), and in vitro trial was carried out to examine the effect of silage and extraction of soluble protein on fermentation characteristics, total gas production and degradation. Soluble protein of silages was extracted with borate-phosphate buffer, and fermentation characteristics, gas production and degradation of silages were estimated by incubating anaerobically the mixed solution of strained rumen fluid and artificial saliva (1:1, v/v) containing dried and ground silages placed in nylon bag at
up to 48h. Soluble protein (SP) content was lowest for RSS as 2.11% in total CP compared to those for other silages. Highest A fraction (NPN) was observed from RS (74.33% of total CP) while those from TS and RSS were relatively low (48%). B2 fraction was relatively higher for RS, RSS and WS than for TS and BS.
fraction was lowest in WS among silages. C fraction (27.07) in RSS was higher than in other silages (1.40~9.93%). pH in incubation solution was increased (P<0.01~P<0.001) for extracted silages up to 12h but decreased (P<0.01) at 48h for non-extracted ones. Contents of ammonia-N (P<0.001) and total VFA (P<0.01~P<0.001) were higher for non-extracted silages than for extracted ones. Acetate proportion was increased (P<0.001) in buffer extracted silages while those of propionate and butyrate were decreased (P<0.001) up to 24h incubation. Increased (P<0.001) total gas production was obtained from non-extracted silages up to 12h while gas production was increased (P<0.01) in extracted ones thereafter. In vitro degradation of dry matter and CP was increased (P<0.001) in non-extracted silages but that of neutral detergent fiber was increased (P<0.001) in extracted ones without difference among silages. Difference in mean values of degradability for each silage prior to- and post extraction with borate buffer, however, was not found among silages. It may be concluded that high NPN content of silages may reduce the protein availability in silages and borate buffer soluble components in silages can stimulate the early stage of fermentation.
Effects of Supplementation of Vitamin A on Fermentation Pattern in the Rumen and Cellulose Degradability Ruminococcus flavefaciens
Ahn, Jong-Ho ; Kim, Bo-Ra ;
Journal of Animal Science and Technology, volume 51, issue 5, 2009, Pages 379~386
DOI : 10.5187/JAST.2009.51.5.379
The aim of this study was tofind out the effects of supplementation of vitamin A to the diets of high or low amounts of concentrates for ruminants. In the first experiment, ruminal fermentation patterns with the data of pH, VFA production and cellulose disappearance rates in the rumen in vitro were investigated. In the second experiment, enzyme activities, gas production and dry matter degradabilities using cellulolytic bacteria, Ruminococcus flavefaciens were investigated. Ruminal pH was higher in low amounts of concentrates than in high amounts of concentrates as expected, however, no significant differences were found. Cellulose disappearance rates improved in vitamin A addition particularly in early incubation time (before 24h) and also the production of volatile fatty acids increased in vitamin A addition. These trends were more evident in diets containing high amounts of concentrates than in low amounts of concentrates and it may indicate that vitamin A is more required in the diets of high amounts of concentrates. In the second experiment, gas production, enzyme activities and dry matter degradabilities using cellulolytic bacteria, Ruminococcus flavefaciens were not different between vitamin A added and non-added diets. Ruminococcus flavefaciens may not require additional vitamin A for its own growth.
Screening and Isolation of Chitinase and Chitosanase Producing Microbes from the Feces of Korean Native Calves Medicated DFMs Including Chitin
Kim, Tae-Il ; Kwon, Eung-Gi ; Kim, Hyeong-Cheol ; Cho, Young-Moo ; Park, Byung-Ki ; Lee, Won-Kyu ; Im, Seok-Ki ;
Journal of Animal Science and Technology, volume 51, issue 5, 2009, Pages 387~394
DOI : 10.5187/JAST.2009.51.5.387
This study was carried out to screen and identify the chitinase and chitosanase producing microorganisms from the feces of calves medicated DFM sincluding chitin in order to do the immune fortification of Korean Native calves. Ten isolates were grown in the medium containing chitin and chitosan that had more than
cfu/g in feces. Among these 10 strains, 2 strains (HANDI 110 and HANDI 309) had the chitinase activities and 2 strains (HANWOO and HANYOO) had the chitosanase activities in calves' feces. They showed no reaction in hemolysis tests by utilizing chitin and chitosan. The results from morphological, physicochemical and genetical identification indicated the HANDI 110 as a strain of Escherichia fergusonii, HANDI 309 was identified as a strain of Acinetobacter parvus, HANWOO was identified as a strain of Comamonas koreensis, and HANYOO as a strain of Chryseobacterium indologenes.
Effects of Supplemented PROSOL® as an Emulsifier on Growth Performance and Carcass Characteristics in Hanwoo Steers of Final Fattening Period
Jeong, Joon ; Hwang, Jeong-Mi ; Seong, Nak-Il ; Kim, Jeong-Bae ; Hwang, Il-Ki ; Kim, Yong-Chul ;
Journal of Animal Science and Technology, volume 51, issue 5, 2009, Pages 395~406
DOI : 10.5187/JAST.2009.51.5.395
Fifty four Hanwoo steers in final fattening period were assigned to 3 groups control, top dressed
as an emulsifier (TP) and DSP group (experimental diet made to down spec of nutrients with
, which is sodium stearoyl-2-lactylate), based on the body weight (647.96
41.31 kg) and months of age (27.3
0.8 mo), and the experiment was conducted to establish the reasonable fattening method of Hanwoo steers for 91 days. Average daily body gains during trial were 0.94, 0.84 and 0.98 kg in control, TP and DSP, respectively (P=0.011). DDMI/ADG of TP group tend to lightly improving compared to control and DSP group (P=0.692). There was no effect of supplementation of the
in concentrates on growth rate, feed efficiency and shrinkage in Hanwoo steers during the short final fattening period. MQI from TP and DSP was not different based on the rib-eye area and carcass weight in carcass than that from control. Back fat thickness tended to be thicker than control (P>0.05). Marbling score, texture and maturity for TP and DSP was abundantly to increase compared to control (P<0.003). There was significantly increment in supplemented
(P<0.0001). The meat quality grade of control, TP and DSP were 2.94, 3.78 and 4.50, respectively. Related to this result, the auction price (carcass/kg) were gained significantly (P<0.003) from control (17,560), TP (18,586) and DSP (19,266 won) so which the monetary return was the highest in DSP and the differences was recognized between TP and DSP. Percentage over 1st grade appeared in control, TP and DSP were 55.4, 88.9 and 100.0%, respectively. These results supported the hypothesis that supplementation of emulsifier improve the marbling score and the carcass quality grade by increased digestibilities of the feed fat in Hanwoo steers in fattening period.
Immuno-specificity of Egg Yolk Antibodies against Bovine Rotavirus and Bovine Coronavirus causing Calf Diarrhea
Lee, Seong ; Woo, Seung-Eun ; Lee, Sang-Rae ; Kim, Jung-Woo ;
Journal of Animal Science and Technology, volume 51, issue 5, 2009, Pages 407~412
DOI : 10.5187/JAST.2009.51.5.407
This study was performed to produce specific egg yolk antibodies against bovine rotavirus (BRV) and bovine coronavirus (BCV) that are major pathogens causing diarrhea in calves. Chickens were immunized with BRV and BCV intramuscularly in the breast muscle by injection 5 times at two weeks interval. At 6 weeks after the first immunization of BRV or BCV, cross reactivity of each serum derived from BRV- or BCV-immunized hens was tested. Each serum antibody against BRV or BCV was reacted with only specific BRV or BCV antigens. Serum and egg yolk-antibody titers of hens against BRV or BCVwere highest at 8~12 weeks after first immunization. Specific serum and egg yolk-antibody titers against BRV were about 104,000 and 107,000, respectively, and those against BCV were about 145,000 and 155,000, respectively. Hemagglutination inhibition titers in the immunized egg yolk antibodies against BRV and BCV were 5,120 and 1,280, respectively, and were
times higher than that in non-immunized control. These results suggested that the immunized egg yolk antibodies could effectively neutralize BRV and BCV.
Effect of Gender-specific Bovine Serum Supplemented Medium on Cell Culture
Lee, Dong-Mok ; Choi, Moon-Seok ; Woo, Gyung-Il ; Shin, Yu-Mi ; Lee, Ki-Ho ; Cheon, Yong-Pil ; Chun, Tae-Hoon ; Choi, In-Ho ;
Journal of Animal Science and Technology, volume 51, issue 5, 2009, Pages 413~420
DOI : 10.5187/JAST.2009.51.5.413
The research has been aimed to investigate the effect of different sera including fetal bovine serum (FBS), male bovine serum (MS), female bovine serum (FS), and castrated-male bovine serum (C-MS) on cell proliferation, follicular maturation and ovulation in vitro. Established cell lines and primary cells were cultured in the culture media supplemented with different sera and cells proliferation was observed by cell counting and MTT assay. The results indicated that cell proliferation was significantly different for different serum source. Proliferation of bovine and human myogenic satellite cells was highest in MS. In contrast, proliferation of breast cancer cells and immune cells were the highest in FS and FBS, respectively. There was no difference in the rate of follicular growth, whereas the rate of ovulation was higher in FBS and C-MS. Finally, the wound healing effect and cell proliferation of 3T3-L1 cells showed that wound healing was fastest in FS and cell proliferation was higher in MS. These results suggest the importance of an optimal serum selection in the experiments involving cell culture system, and gender-specific Hanwoo sera may be used as a substitute to FBS.
Characteristics of Vocalizations of Laying Hen Related with Space in Battery Cage
Son, Seung-Hun ; Shin, Ji-Hye ; Kim, Min-Jin ; Kang, Jeong-Hoon ; Rhim, Shin-Jae ; Paik, In-Kee ;
Journal of Animal Science and Technology, volume 51, issue 5, 2009, Pages 421~426
DOI : 10.5187/JAST.2009.51.5.421
This study was conducted to clarify the characteristics of vocalization of laying hen related with space in battery cage. The size of cages were classified into control (0.30 m
0.55 m, length
height), small (0.21 m
0.55 m) and large (0.30 m
0.55 m) size. Vocalization of 16 individuals of laying hen in each group of Hy-Line Brown (80 week old) were recorded 3 hours per day (10:00am~11:00am, 3:00pm~4:00pm and 7:00pm~8:00pm) using digital recorder and microphone during October 2008 and February 2009. Characteristics of frequency, intensity and duration of vocalization were analyzed by GLM (general linear model) and Duncan's multi-test. There were differences in basic and maximum frequency, and intensity based on analysis of spectrogram and spectrum among different cage sizes. Vocalization of laying hen would be one of the indicators to understand the stress caused by rearing space in batter cage.
Studies on the Isolation and Identification of Xylanase and Mannanase Producing Aspergillus niger
Kim, Byoung-Suk ; Cho, Jin-Kook ; Song, Jin-Ook ; Lee, Hak-Kyo ; Hwang, Seong-Gu ;
Journal of Animal Science and Technology, volume 51, issue 5, 2009, Pages 427~432
DOI : 10.5187/JAST.2009.51.5.427
This study was undertaken to screen a high xylanase and mannanase producing microbes. In the first experiment, screening was undertaken against 50 samples of microorganisms having xylanase and mannanase activities from soil and fallen leaves. The screening process has focused on picking out fungi having high xylanase and mannanase activities under the solid-state fermentation. The xylanase and mannanase activities of 6 screened microbes were 0.9~1.6 unit/mL and 0.2~0.4 unit/mL, respectively, under the submerged fermentation condition. However, under the solid-state fermentation, xylanase and mannanase activities were 103.7~220.0 unit/g and 20.1~40.3 unit/g, respectively. Finally one microbe (E-3) was selected and its xylanase and mannanase activities were 197.3 unit/g and 39.9 unit/g, respectively. The morphological and molecular biological classification of E-3 showed 99% homology with the Aspergillus niger.
A Study on Livestock Products Brand Loyalty of University Students
Kim, Seok-Eun ; Kim, Kon-Joong ;
Journal of Animal Science and Technology, volume 51, issue 5, 2009, Pages 433~440
DOI : 10.5187/JAST.2009.51.5.433
This study was conducted to investigate the experience of purchasing a specific livestock farm product brand, loyalty to a brand, reasons of loyalty, reasons of nonloyalty and intension of paying a premium for a preferred brand according to gender, occupation of parents, a place of residence and income of parents with 408 university students by using a questionnaire and to provide measures for development of livestock farm product brands satisfying both to producers and consumers. According to the results, only the students (25.7%, 105) had bought a specific livestock farm product brand and more female students (33.6%) had purchased a specific brand than male students (21.4%) (p<0.01). For loyalty to a brand, males were more loyal by recording 3.83 points, and students whose parents were farmer showed the highest or 4.17 points (p<0.05). As reasons of loyalty to a brand, female students answered quality (76.6%, 36) and a price (12.8%, 6) in that order and male students considered its name value as the most important reason (26.4%, 14) (p<0.05). In conclusion, experience of buying a livestock farm product brand was found to be a factor increasing university students‘ loyalty to a brand. Although quality and a price affected largely the purchase of a brand, a place to buy it and a price were major factors hindering the purchase.