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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Animal Science and Technology
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Journal DOI :
Korean Society of Animal Science and Technology
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Volume & Issues
Volume 51, Issue 6 - Dec 2009
Volume 51, Issue 5 - Oct 2009
Volume 51, Issue 4 - Aug 2009
Volume 51, Issue 3 - Jun 2009
Volume 51, Issue 2 - Apr 2009
Volume 51, Issue 1 - Feb 2009
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Identification of Differentially Expressed Genes Between Preadipocytes and Adipocytes Using Affymetrix Bovine Genome Array
Yu, Seong-Lan ; Lee, Sang-Mi ; Kang, Man-Jong ; Jeong, Hang-Jin ; Sang, Byung-Chan ; Jeon, Jin-Tae ; Lee, Jun-Heon ;
Journal of Animal Science and Technology, volume 51, issue 6, 2009, Pages 443~452
DOI : 10.5187/JAST.2009.51.6.443
Adipocytes are differentiated from preadipocytes and have large capacity for storing fats inside cells. In cattle, intramuscular fat (IMF) content is one of the major determinants for meat quality and also highly affects market prices, especially in Japan and Korea. In order to profiling differentially expressed genes between intramuscular fibroblast-like cells (preadipocytes) and their differentiated adipocytes, we have established intramuscular fibroblast-like cells from M. longissimus thoracis in Korean cattle (Hanwoo). The differentially expressed genes were selected by comparing these two types of cells ug thecommercially available 23kese two types of cells ug theco. The results indan ced that 206 arecomelements were differentially expressed. Of these, 67 and 94 ks wn genes were up and d wn regulaced, respectively, in adipocytes ug ng both 2-fold difference and Welch's t-test as the cut-off points. The differentially expressed genes identified in this study can be used as good markers for improving meat quality traits with further verification of their biological functions, especially IMF contents in cattle.
Identification of Single Nucleotide Polymorphisms in PRNP Gene of Korean Native Goats
Hoque, Md. Rashedul ; Yu, Seong-Lan ; Yeon, Seong-Heum ; Lee, Jun-Heon ;
Journal of Animal Science and Technology, volume 51, issue 6, 2009, Pages 453~458
DOI : 10.5187/JAST.2009.51.6.453
Prion protein (PRNP) is known to be a causative protein for transmissible spongiform encephalopathy (TSE), a disease occurring in human and animals. Previous results indicate that the genetic variability can affect the resistance and susceptibility of goat scrapie and can give the guideline for reducing the risk of this disease. Until now, 35 single nucleotide polymorphisms (SNPs) were identified in goat PRNP gene from many countries such as Great Britain, Italy, United States of America and Asian countries etc. In this study, SNPs in PRNP gene have been investigated to research the PRNP variations and their possible TSE risks in 60 Korean native goats. Based on the sequencing results, we identified four SNPs and three of those polymorphisms (G126A, C414T and C718T) were synonymous and the A428G polymorphism was non-synonymous which changes the amino acid histidine to arginine. Previously, all of these four SNPs were identified in Asian native goats. Specifically, five polymorphisms were identified in Asian native goats and two of them (G126A and C414T) were silent mutations, and the other SNPs (T304G, A428G and T718C) caused amino acid changes (W102G, H143R and S240P). Comparing with SNP results from other breeds, this study is an initial step to understand resistance and susceptibility of this disease in Korean native goats.
Differential Proteome Expression of In vitro Proliferating Bovine Satellite Cells from Longissimus Dorsi, Deep Pectoral and Semitendinosus Muscle Depots in Response to Hormone Deprivation and Addition
Rajesh, Ramanna Valmiki ; Kim, Seong-Kon ; Park, Mi-Rim ; Park, Min-Ah ; Jang, Eun-Joung ; Hong, Seung-Gu ; Chang, Jong-Soo ; Yoon, Du-Hak ; Kim, Tae-Hun ; Lee, Hyun-Jeong ;
Journal of Animal Science and Technology, volume 51, issue 6, 2009, Pages 459~470
DOI : 10.5187/JAST.2009.51.6.459
The aim of this study was to analyze the proteome of proliferating bovine satellite cells from longissimus dorsi, deep pectoral and semitendinosus muscle depots which had been subjected to hormonal deprivation or addition in culture. For hormone deprivation or addition studies, the cells were either grown in 10% charcoal-dextran stripped fetal bovine serum (CD-FBS) or in 10% FBS supplemented medium. Further to analyze the effect of insulin like growth factor (IGF-1) and testosterone (TS), the cells were grown in 10% CD-FBS containing IGF-1 (10 ng/ml) or TS (10 nM). Results have shown that hormone deprivation had a negative impact on proliferation of the cells from each of the muscle depots. In case of IGF-1 and TS addition, the proliferation levels were low compared with that of the cells grown in 10% FBS. Hence, to gain the insights of the proteins that are involved in such divergent levels of proliferation, the proteome of such satellite cells proliferating under the above mentioned conditions were analyzed using 2D-DIGE and MALDI-ToF/ToF. Thirteen proteins during hormone deprivation and nine proteins from hormone addition were found to be differentially expressed in all the cultures of the cells from the three depots. Moreover, the results highlighted in this study offer a role for each differentially expressed protein with respect to its effect on positive or negative regulation of cell proliferation.
Interferon Tau in the Ovine Uterus
Song, Gwon-Hwa ; Han, Jae-Yong ; Spencer, Thomas E. ; Bazer, Fuller W. ;
Journal of Animal Science and Technology, volume 51, issue 6, 2009, Pages 471~484
DOI : 10.5187/JAST.2009.51.6.471
The peri-implantation period in mammals is critical with respect to survival of the conceptus (embryo/fetus and associated extraembryonic membranes) and establishment of pregnancy. During this period of pregnancy, reciprocal communication between ovary, conceptus, and endometrium is required for successful implantation and placentation. At this time, interferon tau (IFNT) is synthesized and secreted by the mononuclear trophectodermal cells of the conceptus between days 10 and 21~25. The actions of IFNT to signal pregnancy recognition and induce or increase expression of IFNT-stimulated genes (ISGs), such as ISG15 and OAS, are mediated by the Type I IFN signal transduction pathway. This article reviews the history, signaling pathways of IFNT and the uterine expression of several IFNT-stimulated genes during the peri-implantation period. Collectively, these newly identified genes are believed to be critical to unraveling the mechanism(s) of reciprocal fetal-maternal interactions required for successful implantation and pregnancy.
Physiological Effects of Diethylstilbestrol Exposure on the Development of the Chicken Oviduct
Seo, Hee-Won ; Park, Kyung-Je ; Lee, Hyung-Chul ; Kim, Dae-Yong ; Song, Yong-Sang ; Lim, Jeong-Mook ; Song, Gwon-Hwa ; Han, Jae-Yong ;
Journal of Animal Science and Technology, volume 51, issue 6, 2009, Pages 485~492
DOI : 10.5187/JAST.2009.51.6.485
Estrogen has dramatic effects on the development and function of the reproductive tract in mammals. Although diethylstilbestrol (DES) triggers the development of reproductive organs in immature animals, continued exposure to DES induces dysfunction of the female reproductive tract in mice. To investigate the effects of neonatal estrogen exposure on the reproductive tract of female chickens, we implanted DES pellets into the abdominal region of immature female chicks and then examined the effects of DES on the oviducts of both immature chicks and sexually mature chickens (30 weeks old). DES induced mass growth and differentiation of the oviduct in immature chicks. The chick oviduct increased by 2.7- and 29-fold in length and weight, respectively, following primary DES stimulation. In secondary DES stimulation, the length and weight of the chick oviduct increased by 4.5- and 74-fold, respectively. Additionally, DES treatments caused abnormal development of the infundibulum and magnum in hen oviducts. Furthermore, infundibulum abnormality gave rise to unusual ovulation of follicles and resulted in infertility and dysfunction of the magnum, such as less production of egg white proteins. Our results indicate that DES exposure during early developmental stages in chickens has detrimental effects on the development and maintenance of the female reproductive tract after sexual maturation.
Expressional Comparison of Glucose Cotransporter Isoforms in the Rat Epididymis During Postnatal Development
Lee, Dong-Mok ; Seo, Hee-Jung ; Son, Chan-Wok ; Lee, Yong-Ho ; Choi, In-Ho ; Chun, Tae-Hoon ; Cheon, Yong-Pil ; Lee, Ki-Ho ;
Journal of Animal Science and Technology, volume 51, issue 6, 2009, Pages 493~502
DOI : 10.5187/JAST.2009.51.6.493
Glucose is a major source of metabolic fuel and lipid and protein syntheses. Transport of glucose into the cell is regulated by an action of glucose transport.associated transporters, especially solute carriers 2A (Slc2a, protein symbol GLUT). The present study was focused on examination of mRNA expression of various Slc2a isoforms in the epididymis during postnatal development. Total RNAs isolated from different epididymal segments (caput, corpus, and caudal epididymis) were utilized for real-time polymerase chain reaction analyses. Results showed that Slc2a 1, 3, 4, 5, and 8 were expressed in the entire epididymal regions. In addition, the abundance of these Slc2a isoforms' transcripts was different within each epididymal regions. Moreover, the present study showed differential expression of these Slc2a isoforms among different epididymal segments according to postnatal ages. The current study suggests that glucose transport in the epididymis via various Slc2a isoforms would be necessary for maintenance of the epididymal functions.
Effects of Tannic Acid Added to Diets Containing Low Level of Iron on Performance, Blood Hematology, Iron Status and Fecal Microflora in Weanling Pigs
Lee, Seung-Hyung ; Shinde, Prashant ; Choi, Jae-Yong ; Kwon, Ill-Kyong ; Lee, Jeong-Koo ; Pak, Son-Il ; Cho, Won-Tak ; Chae, Byung-Jo ;
Journal of Animal Science and Technology, volume 51, issue 6, 2009, Pages 503~510
DOI : 10.5187/JAST.2009.51.6.503
This study investigated the effects of tannic acid (TA) in the diets for weanling pigs prepared with/without supplemental Fe on performance, hematology, fecal microflora and diarrhea incidence. Limestone and calcium phosphate used in Experiment 1 and 2 were of semi-synthetic and feed-grade quality, respectively; while the trace-mineral premix used in both the experiments was prepared without any added Fe source. In Experiment 1, 108 weaned pigs (Landrace
Duroc, initially 6.46
1.04 kg BW) were allotted to 3 treatments including control (diet added with
and antibiotic), T1 (diet devoid of
and antibiotic) and T2 (T1 diet added with 125 mg/kg TA). Each treatment had 4 replicates with 9 pigs in each pen. Feeding of T1 diet had a negative effect on the performance and plasma Fe status of pigs, while addition of TA to T1 diet resulted in performance of pigs comparable to pigs fed the control diet, reduced diarrhea incidence but had a negative influence on the hematological and plasma Fe indices. Additionally, pigs fed T2 diet had fewer (p<0.05) total anaerobic bacteria, Clostridium spp. and coliforms than pigs fed T1 diet, and greater number of Bifidobacterium spp. and Lactobacillus spp. in feces when compared with pigs fed control and T1 diets. In Experiment 2, 144 weaned pigs (Landrace
Duroc, initially 6.00
1.07 kg BW) were allotted to 4 dietary treatments including control (diet added with
and antibiotic) and diets devoid of supplemental Fe added with antibiotic (An), TA and both (AnTA). Each treatment had 4 replicates with 9 pigs in each pen. Addition of An, TA or both to diets devoid of supplemental Fe did not have any effect on performance, blood hematology and plasma Fe but resulted in reduced (p<0.05) diarrhea incidence and lower (p<0.05) fecal coliform population than pigs fed the control diet. These results suggest that TA has a negative influence on blood hematology and plasma Fe status when diets are inadequate in Fe; however, TA reduced diarrhea incidence and might have antimicrobial activity.
Effects of Thymol, Eugenol and Malate on In vitro Rumen Microbial Fermentation
Kim, Do-Hyung ; Kim, Kyoung-Hoon ; Choi, Chang-Won ; Hong, Seong-Koo ; Seol, Yong-Joo ; Kwon, Eung-Gi ; Kim, Wan-Young ; Nam, In-Sik ; Lee, Sung-Sill ; Oh, Young-Kyoon ;
Journal of Animal Science and Technology, volume 51, issue 6, 2009, Pages 511~520
DOI : 10.5187/JAST.2009.51.6.511
The purpose of this study was to investigate effects of increased levels of eugenol, thymol and malate on pH and the concentrations of VFA, lactate and ammonia-N during in vitro ruminal incubation. One Hanwoo beef steer (741 kg) fitted with a rumen cannula was used and fed 0.5 kg/day rice straw and 10 kg/day corn-based concentrate (ratio of concentrate to rice straw = 95 : 5 on DM basis). Three different doses of thymol, eugenol and malate were used. Treatments of the experiment were as follows: Treatments of thymol were control (1g D-glucose/40ml), T1 (1g D-glucose + 40 mg thymol/40 ml), T2 (1g D-glucose + 50 mg thymol/40 ml) and T3 (1g D-glucose + 60 mg thymol/40 ml). Treatments of eugenol were control (1g D-glucose/40 ml), E1 (1g D-glucose + 55 mg eugenol/40 ml), E2 (1g D-glucose + 65 mg eugenol/40 ml) and E3 (1g D-glucose + 75 mg eugenol/40 ml). Treatments of malate were control (1g D-glucose/40ml), M1 (1g D-glucose + 25 mg malate/40ml), M2 (1g D-glucose + 50 mg malate/40 ml) and M3 (1g D-glucose + 100 mg malate/40 ml). The results of this study showed that eugenol and thymol have improved stability of the ruminal fermentation by decreasing lactic acid concentration and increasing ruminal pH. However, it inhibited the production of total VFA, acetate and propionate. Malate also improved stability of the ruminal fermentation by decreasing lactic acid concentration and increasing ruminal pH, but it had a very little effect on ruminal lactate concentrations and pH. On the other hand, malate did not decrease the concentrations of total VFA, acetate and propionate. Therefore, at the low ruminal pH expected in high-concentrate diets, thymol, eugenol, and malate are potentially useful in Hanwoo finishing diets. Further studies are necessary for determining the effectiveness of these additives on in vivo rumen fermentation and animal performance in Hanwoo finishing steers.
Effect of Irradiation on the Mixture of Egg White Proteins Responsible for Foaming Property
Liu, Xian De ; Han, Rong Xiu ; Jin, Dong-Il ; Lee, Soo-Kee ; Jo, Cheo-Run ;
Journal of Animal Science and Technology, volume 51, issue 6, 2009, Pages 521~526
DOI : 10.5187/JAST.2009.51.6.521
Irradiation of egg white increased foaming ability significantly. To investigate the protein modification by irradiation responsible for the increase of foaming ability, 3 major egg white proteins were purchased and mixed (7.7 g/L ovalbumin, 1.8 g/L ovotransferrin, 0.5 g/L lysozyme) as a model system and irradiated at 0, 2.5, and 5 kGy. The different protein expressions were evaluated using 2-D electrophoresis and it was found that ovotransferrin was cleaved by irradiation and molecular weight and isoelectric point were changed. In addition, many uncharacterized proteins were found and it indicated that irradiation modified proteins randomly but mainly fragmentation was observed. Therefore, it can be concluded that protein fragmentation of 3 major egg white proteins responsible for foaming ability may be the main reason for the improvement of foaming ability.
Isolation, Production, and Characterization of Protease from Bacillus subtilis IB No. 11
Lee, Min-Hyang ; Lee, Kang-Moon ; Choi, Yong-Jin ; Baek, Yeon-Soo ;
Journal of Animal Science and Technology, volume 51, issue 6, 2009, Pages 527~536
DOI : 10.5187/JAST.2009.51.6.527
A potent protein degrading bacterium was isolated from soil samples of different environments. Polyphasic taxonomic studies and phylogenetic 16S rRNA sequence analyses led to identify the isolate IB No. 11 as a strain of Bacillus subtilis. The isolated strain was recognized to produce protease constitutively, and the maximum production (1.64 units/ml) was attained in a shake flask culture when the isolate was grown at
, for 32 h in basal medium supplemented with starch (0.25%) and gelatin (1.25%) as sole carbon and nitrogen source, respectively. The optimum pH and temperature for the protease activity were determined to be pH 7.0 and
enhanced remarkably the protease activity but neither showed positive effect on the protease's thermal stability. In addition, it was observed that the protease was fairly stable in the pH range of 6.5-8.0 and at temperatures below
, and it could be a good candidate for an animal feed additive. The inhibition profile of the protease by various inhibitors indicated that the enzyme is a member of serine-proteases. A combination of UV irradiation and NTG mutagenesis allowed to develop a protease hyper-producing mutant strain coded as IB No. 11-4. This mutant strain produced approximately 3.23-fold higher protease activity (6.74 units/mg) than the parent strain IB No. 11 when grown at
for 32h in the production medium. The protease production profile of the selected mutants was also confirmed by the zymography analysis.
Seasonal and Regional Effects on Milk Composition of Dairy Cows in South Korea
Nam, Ki-Taeg ; Kim, Ki-Hyun ; Nam, In-Sik ; Abanto, Oliver D. ; Hwang, Seong-Gu ;
Journal of Animal Science and Technology, volume 51, issue 6, 2009, Pages 537~542
DOI : 10.5187/JAST.2009.51.6.537
For a period of over 6 years, more than 160,000 milk samples were collected and analyzed to determine the influence of different seasonal temperatures and geographic regional location on milk composition in South Korea. Fat, protein, lactose, non fat milk solids (NFMS) and total solids (TS) contents were significantly higher among dairy cows milked in winter season than other seasons (p<0.05). In contrast, freezing point (FP), milk urea nitrogen (MUN) and somatic cell count (SCC) were significantly higher in summer season than other seasons (p<0.05). The average SCC in the autumn season was
/ml, which was lower than any other seasons (p<0.05). These results may be due to the changes in temperature during different seasons. Meanwhile, milk produced by dairy cows in central region had higher fat, protein, lactose, NFMS, TS and MUN and had lower SCC compared to other regions (p<0.05). Fat, TS, FP, MUN and citric acid in northeast region were lower than other regions (p<0.05). The SCC was significantly higher in southeast region than those of other regions (p<0.05). As a result, it might be possible that the differences in feeding management in each different region may affect the milk composition. In conclusion, present results indicated that milk composition is clearly influenced by both season and regional location. Therefore, based on these results, development of different feeding systems, according to season and region is needed to produce high quality and satiable milk production.