Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Animal Science and Technology
Journal Basic Information
Journal DOI :
Korean Society of Animal Science and Technology
Editor in Chief :
Volume & Issues
Volume 54, Issue 6 - Dec 2012
Volume 54, Issue 5 - Oct 2012
Volume 54, Issue 4 - Aug 2012
Volume 54, Issue 3 - Jun 2012
Volume 54, Issue 2 - Apr 2012
Volume 54, Issue 1 - Feb 2012
Selecting the target year
Expression of Ski in the Follicles of eCG-primed Immature Hypophysectomized Rat Ovary
Ko, Yeoung-Gyu ; Kim, Dong-Hun ; Park, Soo-Bong ; Kim, Sung-Woo ; Do, Yoon-Jung ; Kim, Hyun ;
Journal of Animal Science and Technology, volume 54, issue 3, 2012, Pages 151~156
DOI : 10.5187/JAST.2012.54.3.151
Sloan-Kettering virus gene, a product of a cellular protooncogene c-Ski is a unique nuclear pro-oncoprotein and belongs to the Ski/Sno proto-oncogene family. The aim of the present study was to locate Ski protein in rat ovaries in order to find insights into the possible involvement of Ski in follicular development and atresia. Ovaries obtained on the day of estrus were subjected to immunohistochemical analysis for Ski and PCNA in combination with TUNEL. Ski was expressed in granulosa cells that were positive for TUNEL, but negative for PCNA, regardless of the shape and size of the follicles. Expression of Ski in (TUNEL)-positive granulosa cells, but not in (PCNA)-positive granulosa cells, was verified in immature hypophysectomized rats having a single generation of developing and atretic follicles by treatment with equine chorionic gonadotropin (eCG). These results indicate that Ski is profoundly expressed in the granulosa cells of atretic follicles, but not in growing follicles. Based on the present findings, Ski has maybe played plays a role in apoptosis of granulosa cells during follicular atresia.
Expressional Profiling of Molecules Associated with Epigenetic Methylation-Related Fertility in the Rat Testis during Postnatal Period
Seo, Hee-Jung ; Lee, Seong-Kyu ; Baik, Haing-Woon ; Lee, Ki-Ho ;
Journal of Animal Science and Technology, volume 54, issue 3, 2012, Pages 157~163
DOI : 10.5187/JAST.2012.54.3.157
The male reproduction is precisely controlled by a number of intrinsic and extrinsic factors. These factors usually involve in expressional regulation of various molecules influencing on sperm production in the testis. A number of ways are employed to control the transcription of specific genes, including epigenetic modifications of DNA and histone molecules. DNA methylation of CpG dinucleotides is a commonly used regulatory mechanism for testicular genes associated with the fertility. Previous studies have demonstrated the infertility induced by improper DNA methylation of these genes. In the present research, we attempted to determine transcriptional expression of some of these genes in the rat testis at different postnatal ages using real-time PCR analysis. These genes include neurotrophin 3 (Ntf3), insulin-like growth factor II (Igf2), JmjC-domain-containing histone demethylase 2A 1 (Jhm2da), paired box 8 transcription factor (Pax8), small nuclear ribonucleoprotein polypeptide N (Snrpn), and 5,10-methylenetetrahydrofolate reductase (Mthfr). The expression levels of Ntf3, Igf2, and Snrpn genes were the highest at the neonatal age, followed by transient decreases at the prepubertal age. Expression of Jhm2da and Mthfr genes were continuously increased from the neonate to 1 year of age. The levels of Pax8 mRNA at the early ages were higher than those at the later ages of postnatal development. These findings suggest that expression of some fertility-associated testicular genes in the rat during postnatal period could be differentially regulated by the control of the degree of DNA methylation.
The Estrous Cycle and Induction of Ovulation in Mares
Yoon, Min-Jung ;
Journal of Animal Science and Technology, volume 54, issue 3, 2012, Pages 165~174
DOI : 10.5187/JAST.2012.54.3.165
For horse breeders or managers, it is critical to understand the estrous cycle of mares. Breeding of mares cannot be successfully achieved throughout the whole year as mares breed seasonally. Mares are only able to breed when day length is more than 16 h, and this period is known as the breeding season. Their estrous cycle is approximately 21 days with 5-7 days of estrus and 14 to 15 days of a diestrus period. The estrous cycle of the mare is mainly controlled by gonadotropins, which control follicular development and ovulation. Mares exhibit unique ovulatory events which are not observed in other species. A LH surge occurs for several days, with levels of LH reaching their peak after ovulation. The LH level at the time of LH peak is lower than most other species. The unique anatomical structure of the ovaries of mares is known to limit the number of eggs ovulated. Several attempts have been made to develop chemical/hormonal agents which might be used to manipulate the timed ovulation of mares. Agents that have been tested include hCG, native GnRH, Deslorelin (Ovuplant, GnRH-agonist), Buserelin (GnRH analogue), equine pituitary extracts and equine chorionic gonadotropin (eCG or PMSG). However, the function, purity or stability of these agents is not reliable. Recombinant equine LH, an alternative agent for the timed ovulation, has been developed and tested for its biological activities, through the use of both in vitro and in vivo experiments. The reLH was suggested to be a reliable agent in inducing ovulation within 48 h after being administered through injection, when the size of dominant follicle is 35 mm in diameter.
Relationships of Muscle Fiber Characteristics to Dietary Energy Density, Slaughter Weight, and Muscle Quality Traits in Finishing Pigs
Jeong, Jin-Yeon ; Kim, Gap-Don ; Ha, Duck-Min ; Park, Man-Jong ; Park, Byung-Chul ; Joo, Seon-Tea ; Lee, C.-Young ;
Journal of Animal Science and Technology, volume 54, issue 3, 2012, Pages 175~183
DOI : 10.5187/JAST.2012.54.3.175
The present study was conducted to investigate the relationships of muscle fiber characteristics to dietary energy density [3.0(Low-E) vs. 3.2 (Med-E) Mcal DE/kg)] and slaughter weight [SW; 110, 125, and 138 kg] in finishing pigs (gilt vs. barrow) using a
factorial treatment design. Forty-one longissimus dorsi muscle (LM) samples were analyzed histochemically, with growth performance and physicochemical data for the 41 animals and their LM out of 192 animals and 72 LM used in a previous study retrospectively included. The ADG was less (P<0.01) in the Low-E than in the Med-E group (0.93 vs. 0.73 kg) whereas lightness (
) and redness (
) of LM were greater in the Low-E group SW did not influence these variables. The diameter and perimeter of the type I (slow-oxidative), type IIA (fast oxido-glycolytic) and type IIB (fast glycolytic) fibers increased with increasing SW whereas densities of the fibers decreased. However, the number and area percentages of the fiber types were not influenced by SW or dietary energy density. The percentage and per-
density of type IIB fibers were negatively correlated with SW (r = -0.33 and -0.57, with P<0.05 and <0.01, respectively), whereas type I fiber number percentage was positively correlated with SW (r = 0.31; P<0.05). Marbling score was negatively correlated (P<0.05) with type I (r = -0.36) and type IIB (r = -0.39) fiber densities. The
was correlated (P<0.01) with both type I and type IIB fiber number percentages in the opposite way (r = 0.42 and -0.47, respectively). However,
(lightness), drip loss and
were not correlated with the fiber number percentage or density of any fiber type. Collectively, results indicate that muscle fibers grow by hypertrophy during the late finishing period, but that fiber characteristics other than the size are not significantly influenced by dietary energy density or SW.
Effects of Garlic and Thyme Extracts on Growth Performance and Carcass Characteristics of Broiler Chicks
Amouzmehr, Anvar ; Dastar, Behrouz ; Nejad, Jalil Ghassemi ; Sung, Kyung-Il ; Lohakare, Jayant ; Forghani, Fereidoun ;
Journal of Animal Science and Technology, volume 54, issue 3, 2012, Pages 185~190
DOI : 10.5187/JAST.2012.54.3.185
A total of 800 one-day-old chickens (Cobb 500) were obtained from a local hatchery and allocated randomly to five dietary treatments. This study conducted for 42 d. Treatment groups included 1) CON (control), 2) T0.3 (0.3% of thyme extract), 3) T0.6 (0.6% of thyme extract), 4) G0.3 (0.3% of garlic extract) and 5) G0.6 (0.6% of garlic extract). Thyme extract included
Carvacrol and 400 mg/ml thymol while garlic extract included 100 mg/ml elastin. There were no significant effects among the treatments on weight gain, feed intake and feed conversion ratio over the entire trial. Also, no significant differences were observed between CON group and other treatments in plasma parameters (cholesterol, triglyceride and HDL) and hematocrit. Sex of chickens had no effect on blood parameters. Supplementation of herb extracts did not affect carcass characteristics including carcass yield, breast, thigh and abdominal fat. There were no significant differences observed in carcass characteristics between males and females fed with different levels of herb extracts. In conclusion, supplementation of garlic and thyme extracts as feed additives at two levels of 3 and 6% in the present study did not improve performance and carcass traits of broiler chicks.
Evaluation of a Dielectric Barrier Discharge Plasma System for Inactivating Pathogens on Cheese Slices
Lee, Hyun-Jung ; Jung, Samooel ; Jung, Hee-Soo ; Park, Sang-Hoo ; Choe, Won-Ho ; Ham, Jun-Sang ; Jo, Cheorun ;
Journal of Animal Science and Technology, volume 54, issue 3, 2012, Pages 191~198
DOI : 10.5187/JAST.2012.54.3.191
The objective of this study was to evaluate the potential use of a dielectric barrier discharge (DBD) plasma system to improve microbial safety of sliced cheese. The atmospheric pressure plasma (APP) effect on visual appearance and a sensory evaluation were also carried out. The number of Escherichia coli inoculated on cheese slices decreased by 0.09, 0.47, 1.16 and 1.47 log cycles with helium (4 liters/min [lpm]) and 0.05, 0.87, 1.89 and 1.98 log cycles with He/
mixture (4 lpm/15 standard cubic centimeters per minute), after being treated with plasma for 1, 5, 10, and 15 min, respectively. Significant reductions were also observed in Staphylococcus aureus inoculated onto cheese slices ranging from 0.05 to 0.45 log cycles with He and from 0.08 to 0.91 log cycles with He/
-treated samples, respectively. Adding oxygen resulted in a significant increase in inactivation of both pathogens. No visible change in the plasma-treated cheese slices was observed even though the instrumental analysis showed a significant decrease in the
-value and an increase in the
-value. The cheese slices were damaged after 10 and 15 min of plasma treatment. In addition, significant reductions in sensory quality including flavor, odor, and acceptability of plasma-treated cheese slices were observed. The results indicate that the DBD plasma system has potential for use in sanitizing food products, although the effect was limited. Further development of the APP system is necessary for industrial use.
Effects of a Dietary Fermented Mushroom (Flammulina velutipes) By-Product Diet on Pork Meat Quality in Growing-Fattening Berkshire Pigs
Chu, Gyo-Moon ; Kang, Suk-Nam ; Yang, Jeong-Mo ; Kim, Hoi-Yun ; Song, Young-Min ;
Journal of Animal Science and Technology, volume 54, issue 3, 2012, Pages 199~207
DOI : 10.5187/JAST.2012.54.3.199
This study was carried out to investigate the effects of fermented mushroom (Flammulina velutipes) by-products on meat quality characteristics in fattening Berkshire pigs. The fermented diet mainly contained 40.0% mushroom by-products, 26.0% rice bran, and 20.0% formula feed and was fermented for 5 d. The basal diet for the control (C) was substituted with 10% (T1), 30% (T2), 50% (T3), and 70% (T4) fermented diet. Warner-Bratzler shear forces (WBSF) were significantly lower (P < 0.05) in treatments than that in C. The meat color (lightness, redness, and yellowness) was significantly lower (P < 0.05) in treatments than that in C, whereas fat color (redness and yellowness) was significantly higher in treatments than that in C (P < 0.05). The compositions of palmitoleic acid and arachidonic acid were significantly higher (P < 0.05) in T4 than that in C. The amino acid composition of longissimus dorsi (LD) and the sensory evaluation of cooked meat were not affected by diet type. In conclusion, a diet of fermented mushroom by-products increased pH and backfat color, but decreased cooking loss, WBSF, and meat color of LD in growing-fattening Berkshire pigs.
Evaluation of Egg Quality Traits in the Wholesale Market in Sri Lanka during the Storage Period
Jayasena, Dinesh D. ; Cyril, Hewa W. ; Jo, Cheorun ;
Journal of Animal Science and Technology, volume 54, issue 3, 2012, Pages 209~217
DOI : 10.5187/JAST.2012.54.3.209
The objective of this study was to assess the external and internal quality traits of eggs in wholesale egg market in Colombo, Sri Lanka and the effect of storage period in egg quality traits in the same market after receiving the eggs. First, a total of 482 fresh eggs were randomly collected from the above market and external egg quality traits were determined and recorded according to the definitions given in the Specification for Chicken Egg SLS 959:1992. After that, a total of 288 fresh eggs were randomly collected and various external and internal egg quality traits were measured and recorded according to the standard procedures over a storage period of 1, 3 and 5 d after receiving the fresh eggs to the wholesale market. Information about the shops was also collected using a pretested questionnaire. Using the recorded data, shape index, Haugh unit, albumen index and yolk index were calculated for each egg. Average weight, width, length and shape index of the eggs in the sample was 59.96 g, 4.33 cm, 5.78 cm and 75.03, respectively. Average shape index value was much closer to the standard value of 74. From the total sample 80.5% eggs had a normal and sound shape. However only 60.37% of the eggs are in the desirable quality range specified in SLS 959:1992, when the overall shell quality of the sample is considered based on shell cleanliness, defects and shape. The results of the current study indicated that eggs had significant (p<0.05) deterioration of all internal quality parameters tested with increasing storage time. However the effect was not significant (p>0.05) between the storage periods of 3 and 5 d after receiving eggs except for yolk color and yolk height. Desirable category of eggs had reduced and rejections had increased with the storage period. Main problems associated with the particular market were less space availability, higher percentages of dirty eggs, unavailability of proper packaging materials, no standard packaging system for eggs, and not implementing a standard grading system for eggs. Therefore the results of this study suggest that proper egg handling and storage conditions such as low temperature storage may be implemented to increase the proportion of desirable quality eggs in the above market.
Effect of Gender-Specific Adult Bovine Serum on Gene Expression During Myogenesis
Lee, Eun-Ju ; Pokharel, Smritee ; Kim, Jie-Hoe ; Nam, Sang-Sup ; Choi, In-Ho ;
Journal of Animal Science and Technology, volume 54, issue 3, 2012, Pages 219~226
DOI : 10.5187/JAST.2012.54.3.219
Gender specificity in muscle growth and development is well known. Genesis of muscle is dependent on proliferation and differentiation potential of resident myogenic satellite cells (MSCs) present in muscle fibers. Multipotential capacity of forming myocyte, osteocyte, and adipocyte like cell makes MSCs a unique stem cell. To understand the molecular mechanism involved in determination of muscle quality due to difference in hormone concentration of different gender of animals, MSCs were isolated from bovine skeletal muscle and cultured in male, female, and castrated serum supplemented media. DNA microarray used consisted of 24,000 spots with 70 mer oligo in each spot. A total of 88 genes were up-regulated and 551 genes were down-regulated by more than two fold. Among up-regulated gene, 33, 34, and 21 genes were found up-regulated in cells grown in male, female, and castrated serum, respectively. Interestingly, male serum showed 4, female 11 and castrated male showed 4 genes expressed highly in each gender. Further study on the highly up-regulated gene may unfold the mystery of gender specificity found in muscle development. Also, the identification of differentially expressed genes in gender-specific serum will add information on infrastructure of bovine genome research.
Effect of Horse Riding on Balancing Ability in Children with Cerebral Palsy
Kang, Ok-Deuk ; Kang, Min-Soo ; Kang, An-Na ;
Journal of Animal Science and Technology, volume 54, issue 3, 2012, Pages 227~231
DOI : 10.5187/JAST.2012.54.3.227
This study was conducted to investigate the effect of a horse riding program on balancing ability in children with cerebral palsy. Eleven children (five males and six females) diagnosed with cerebral palsy participated. The horse riding exercises (walking and trotting) were conducted twice per week for 30 minutes during 24 weeks. Balancing ability was measured three times at pre, mid, and post-test using an air pad by the same physical therapist. The data were analyzed using a two-way repeated-measures analysis of covariance with time (0, 12, and 24 weeks) using SPSS version 18.0. A comparison of horse riding between patients with hemiplegia and paraplegia was conducted with the Willcoxon signed-ranktest at a predetermined probability rate of 5%. The results showed a significant increase in balancing ability after horse riding than that before horse riding (p<0.01). The average balancing score increased greater in males (
) than that in females (
) after the horse riding exercise program compared to that before the program (p < 0.05). Thus, horse riding exercise was considered an effective to improve balance in children with cerebral palsy. These results provide useful basic data for horse riding for the disabled.