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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Animal Science and Technology
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Journal DOI :
Korean Society of Animal Science and Technology
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Volume & Issues
Volume 54, Issue 6 - Dec 2012
Volume 54, Issue 5 - Oct 2012
Volume 54, Issue 4 - Aug 2012
Volume 54, Issue 3 - Jun 2012
Volume 54, Issue 2 - Apr 2012
Volume 54, Issue 1 - Feb 2012
Selecting the target year
Gene Expression Profiling in Hepatic Tissue of two Pig Breeds
Jang, Gul-Won ; Lee, Kyung-Tai ; Park, Jong Eun ; Kim, Heebal ; Kim, Tae-Hun ; Choi, Bong-Hwan ; Kim, Myung Jick ; Lim, Dajeong ;
Journal of Animal Science and Technology, volume 54, issue 6, 2012, Pages 383~394
DOI : 10.5187/JAST.2012.54.6.383
Microarray analyses provide information that can be used to enhance the efficiency of livestock production. For example, microarray profiling can potentially identify the biological processes responsible for the phenotypic characteristics of porcine liver. We performed transcriptome profiling to identify differentially expressed genes (DEGs) in liver of pigs from two breeds, the Korean native pigs (KNP) and Yorkshire pigs. We correctly identified expected DEGs using factor analysis for robust microarray summarization (FARMS) and robust multi-array average (RMA) strategies. We identified 366 DEGs in liver (p<0.05, fold-change>2). We also performed functional analyses, including gene ontology and molecular network analyses. In addition, we identified the regulatory relationship between DEGs and their transcription factors using in silico and qRT-PCR analysis. Our findings suggest that DEGs and their transcription factors may have a potential role in adipogenesis and/or lipid deposition in liver tissues of two pig breeds.
Estimation of Growth Curve Parameters for Body Weight and Length in Miniature Pigs
Kang, Hyun Sung ; Nam, Ki Chang ; Cabling, Meriam M. ; Lee, Myeong Seop ; Choi, Te Jung ; Yoon, Jong Taek ; Seo, Kang Seok ;
Journal of Animal Science and Technology, volume 54, issue 6, 2012, Pages 395~400
DOI : 10.5187/JAST.2012.54.6.395
This study was conducted to estimate the growth curve parameters for the body weight (BW) and body length (BL) of miniature pigs in Korea. Growth curve parameters were estimated through a nonlinear regression model using Gompertz, Logistic, and von Bertalanffy methods. A total of 25 piglets were measured monthly from birth up to 15 months of age to estimate both body weight and length. Results showed that the estimated average values for the body weight (body length) were 31.83 kg (58.77 cm) for the mature weight (A), 3.06 (1.74) for the growth ratio (
), and 0.28 (0.52) for the maturing rate (
). Average inflection points showing maximum growth rate estimated each month for body weight were 3.97 kg and 11.70 cm, while for the body length were 1.06 kg and 21.61 cm. Moreover, the estimated maturation rates of the body weight and length for the group of Sire 1 were 0.22 and 0.40 respectively, whereas for the group of Sire 2 these values were 0.34 and 0.39. On the other hand, for the groups of Dam 1, Dam 2, and Dam 3, maturation rates for their body weights were 0.26, 0.28 and 0.33 respectively, while for their body lengths these values were 0.43, 0.37, and 0.38, respectively. The study also indicated a negative relationship between the values of mature weight and maturity rate for the body weight will result to a higher inflection point which is in contrast for the body length where results show that a positive relationship between the values of mature length and the maturity rate will result to a higher inflection point. Furthermore, the growth performance of miniature pig varies across stages but using these estimated growth curve parameters could improve the genetic traits of miniature pig.
ISAG-recommended Microsatellite Marker Analysis Among Five Korean Native Chicken Lines
Choi, Nu-Ri ; Hoque, Md. Rashedul ; Seo, Dong-Won ; Sultana, Hasina ; Park, Hee-Bok ; Lim, Hyun-Tae ; Heo, Kang-Nyeong ; Kang, Bo-Seok ; Jo, Cheorun ; Lee, Jun-Heon ;
Journal of Animal Science and Technology, volume 54, issue 6, 2012, Pages 401~409
DOI : 10.5187/JAST.2012.54.6.401
The objective of this study was to determine genetic variation of five Korean native chicken lines using 30 microsatellite (MS) markers, which were previously recommended by ISAG (International Society for Animal Genetics). The initial study indicated that two microsatellite markers, MCW0284 and LEI0192, were not amplified in these lines and excluded for further analysis. Twenty eight microsatellite markers were investigated in 83 birds from five Korean native chicken lines. The identified mean number of alleles was 4.57. Also, the expected, observed heterozygosity (He, Ho) and polymorphism information content (PIC) values were estimated in these markers and they ranged from 0.31~0.868, 0.145~0.699, and 0.268~0.847, respectively. The results were used for the discrimination of five chicken lines using genetic distance values and also neighbor-joining phylogenetic tree was constructed. Based on the He and PIC values, eighteen markers are enough for the discrimination of these Korean native chicken lines for the expected probability of identity values among genotypes of random individuals (PI), random half sibs (
) and random sibs (
). Taken together, these results will help the decision of conservation strategies and establishment of traceability system in this native chicken breed. Also, the use of ISAG-recommended microsatellite markers may indicate that the global comparison with other chicken breeds is possible.
Investigation of KIT Gene Polymorphisms in Korean Cattle
Hoque, Md. Rashedul ; Lee, Seung-Hwan ; Lim, Da-Jeong ; Cho, In-Cheol ; Choi, Nu-Ri ; Seo, Dong-Won ; Lee, Jun-Heon ;
Journal of Animal Science and Technology, volume 54, issue 6, 2012, Pages 411~418
DOI : 10.5187/JAST.2012.54.6.411
KIT gene is the major causative gene for coat color variation in diverse animal species. This gene regulates melanocyte migration from the neural crest to target tissues and the mutation of this gene can affect dominant white phenotypes in animals. Because this gene has a major influence for the coat color variation, single nucleotide polymorphisms (SNPs) in 14 Korean cattle (Hanwoo) and 5 Holstein individuals were investigated. The Hanwoo DNA samples included three different colored (5 Black, 5 Yellow and 4 Stripe) animals. Total 126 polymorphisms have been identified and 23 of them are located in the exon region. Also, 5 bp (TTCTC) and 3 bp (TCT) intronic indels in intron 3 and intron 5, respectively, were identified. Out of 23 exonic polymorphisms, 15 SNPs are the missense mutations and the rest of the SNPs are silence mutations. The neighbor-joining phylogenetic tree was constructed for the different colored animals using the obtained KIT gene sequences. Holstein breed showed a clear breed-specific cluster in the phylogenetic tree which is differed from Hanwoo. Also, three colored Hanwoo animals were not discriminated among the breeds. The KIT gene polymorphisms identified in this study will possibly give some solutions for the color variations in cattle with further verifications.
Examination of the Presence and Differential Expression of Connexin Isoforms in the Urinary Bladder of the Male Rat During Postnatal Period
Lee, Ki-Ho ;
Journal of Animal Science and Technology, volume 54, issue 6, 2012, Pages 419~425
DOI : 10.5187/JAST.2012.54.6.419
The coordinated action of the detrusor muscle cells in the urinary bladder is governed by cell-cell communication through gap junction, consisted of connexin (Cx) molecules. Even though a number of researches have been mostly focused on expressional changes of a few Cx isoforms in clinically dysfunctional condition of the bladder, less attention has been paid for investigation of Cx isoforms present in the bladder. Using real-time PCR analysis, the present study examined Cx isoforms expressing in the male rat bladder during postnatal period. Also, expressional patterns of Cx isoforms were evaluated in the bladder at different postnatal ages. Of a total of 13 Cx isoforms tested in the present study, we were able to detect mRNAs of 6 Cx isoforms in the rat urinary bladder, including Cxs 31, 31.1, 32, 37, 40, and 45. The transcript levels of Cxs 31, 31.1, 37, 40, and 45 were gradually increased from 1 week of age until 25 days of age, followed by transient decreases at 45 days of age. However, abundance of Cx32 transcript was drastically increased at 15 days of age, followed by a sharp drop at 45 days of age. These results indicate that differential expression of Cx isoforms in the bladder during postnatal development would be necessary for maintaining proper function of the bladder. A question remains to be answered if significant decreases of transcript levels of some Cx isoforms at the elderly are associated with age-dependent dysfunction of the bladder.
Effects of the Low-Crude Protein and Lysine (Low CP/lys) Diet and a Yeast Culture Supplemented to the Low CP/lys Diet on Growth and Carcass Characteristics in Growing-finishing Pigs
Ha, Seung-Ho ; Park, Byung-Chul ; Son, Seung Won ; Ha, Duck-Min ; Lee, C. Young ;
Journal of Animal Science and Technology, volume 54, issue 6, 2012, Pages 427~433
DOI : 10.5187/JAST.2012.54.6.427
The present study was performed to investigate long-term effects of the low-crude protein and lysine (low CP/lys) diet and a yeast culture supplemented to the low CP/lys diet on growth and carcass characteristics in growing-finishing pigs. Forty-five gilts and 45 barrows weighing approximately 25 kg born to Yorkshire
Landrace dams and Duroc sires were allocated to nine pens, with five gilts and five barrows assigned per pen. Every three pens received CP/lys-rich grower and finisher diets (control), low CP/lys grower and finisher (basal), or the low CP/lys grower and finisher supplemented (2%) with a yeast culture providing
Saccharomyces cerevisiae cells/kg diet (yeast) for 31 and 79 days, respectively. The ADG was less in the low CP/lys (basal + yeast) group than in the control group (P<0.01) during both grower (0.59 vs. 0.70 kg) and finisher (0.75 vs. 0.89 kg) phases. However, marbling score was greater (P<0.05) in the low CP/lys group vs. control (3.86 vs. 3.12) at slaughter, which resulted in a twice percentage of the
-quality grade carcasses in the former compared with that for the latter. Supplementation of the yeast culture to the basal diet caused a decrease in ADG during the grower phase (P<0.01), but not during the finisher phase (0.64 vs. 0.53 kg and 0.73 vs. 0.77 kg for the basal vs. yeast group during the grower and finisher phases, respectively), without influencing the marbling score or percentage of the
-quality grade carcasses. In conclusion, results indicate that long-term provision of the low CP/lys diet has a beneficial effect on marbling and carcass quality grade as well as a negative effect on weight gain and that the yeast culture supplemented to the low CP/lys diet has no beneficial effect on weight gain or carcass quality.
Isolation and Identification of Lactic Acid Bacteria from Spent Mushroom Substrate for Silage Making and Determination of Optimal Medium Conditions for Growth
Kim, Young-Il ; Kwak, Wan-Sup ;
Journal of Animal Science and Technology, volume 54, issue 6, 2012, Pages 435~442
DOI : 10.5187/JAST.2012.54.6.435
This study was conducted to isolate and identify the lactic acid bacteria (LAB) from spent mushroom substrates (SMS) for the effective anaerobic fermentation to utilize SMS as an animal feed and to determine the optimal medium conditions for their growth. At first, a total of 23 strains were isolated from the ensiled SMS based on the LAB counts and pH tested. Then, a total of 16 strains which rapidly produce lactate and decreased the pH, were selected for a screening test. The optical density (OD), pH, and yellow clear zone were tested for the selected 16 strains. Among the strains, KU5 strain had wider yellow clear zone and lower pH and KU13 strain had higher OD at 24 hr of incubation and wider yellow clear zone compared to other strains and control strain (Lactobacillus plantarum KCCM 12116). Accordingly, KU5 and KU13 strains were finally selected. The KU5 and KU13 were identified as Lactobacillus plantarum by the 16S rRNA sequencing. The KU5 strain was named as Lactobacillus plantarum KU5, and the KU13 strain was named as Lactobacillus plantarum KU13. Lactobacillus plantarum KU5 and Lactobacillus plantarum KU13 were registered at the National Center for Biotechnology Information (NCBI). Access number of Lactobacillus plantarum KU5 was HQ542227 and that of Lactobacillus plantarum KU13 was HQ542228. The optimal medium conditions for growth of KU5 and KU13 were soybean meal 2% and formulated feed 2%, respectively.
Comparison of Blood Metabolites and Enzyme Activities at Different Slaughter Ages of Hanwoo Cattle
Kim, Do Hyung ; Kim, Kyoung Hoon ; Nam, In Sik ; Kim, Wan Young ; Yeo, Joon Mo ; Lee, Sung Sill ; Ju, Jong Cheol ; Oh, Young Kyoon ;
Journal of Animal Science and Technology, volume 54, issue 6, 2012, Pages 443~448
DOI : 10.5187/JAST.2012.54.6.443
This study was conducted to investigate changes of blood metabolites and enzyme activities at different slaughter ages of Hanwoo cattle. Blood samples were taken from six hundred thirty two steers (24~33 months of age) and one hundred twenty eight bulls (17~24 months of age) by venipuncture from caudal vein immediately prior to slaughter. Glucose concentrations were linearly increased (P<0.001) with increases of the slaughter age in steers, and blood urea nitrogen (BUN) concentrations were linearly decreased (P<0.001) with increases of the slaughter age in steers. Likewise, glucose and albumin concentrations in bulls were linearly decreased (P=0.009 and P<0.001, respectively) with increases of the slaughter age. BUN, cholesterol, triglycerides, and total cholesterol concentrations were linearly increased (P<0.001, 0.018 and 0.002, respectively), and creatinine concentrations were quadratically increased (P=0.009) with increases of the slaughter age. Activity of gamma glutamyltransferase (
-GT) and aspartate aminotransferase (AST) was linearly increased (P<0.001) with increases of the slaughter age in both steers and bulls, whereas alanine aminotransferase (ALT) was not affected by increasing slaughter age. In addition, the concentrations of
-GT and AST were higher (P<0.05) for bulls than those of steers at 24 months of age. Increased levels of
-GT and AST indicate impaired liver function; it may be associated with increases of concentrate level which is a concomitant of the extended fattening periods in Hanwoo feeding. In conclusion, the results in the present study may be one of the useful information for diagnosis of the metabolic disorder in Hanwoo cattle.
Effects of Plane of Nutrition on Growth Performance and Meat Quality Traits in Finishing Pigs
Ha, Duck-Min ; Park, Byung-Chul ; Park, Man Jong ; Song, Young Min ; Jin, Sang Keun ; Park, Jae Hong ; Lee, C. Young ;
Journal of Animal Science and Technology, volume 54, issue 6, 2012, Pages 449~454
DOI : 10.5187/JAST.2012.54.6.449
The present study was performed to investigate the effects of the plane of nutrition on growth performance and meat quality traits, especially marbling and PSE (pale, soft, and exudative), in finishing pigs. One hundred and four barrows and 102 gilts born to Yorkshire
Landrace dams and Duroc sires were randomly allocated to 16 pens at approximately 80-kg body weight, with 13 animals housed per pen except for two pens. Each eight pens received a diet containing either 3.4 Mcal ME/kg and 0.98% total lysine (high plane) or 3.1 Mcal ME/kg and 0.81% total lysine (medium plane). All animals were slaughtered at approximately 112 kg BW on an average, after which the Longissimus muscle (LM) was analyzed physicochemically. The ADG, ADFI, gain:feed as well as marbling score did not differ between the two dietary groups, whereas backfat thickness was greater in the high-plane group than in the medium-plane group (24.1 vs. 22.5 mm P<0.05). No LM exhibited PSE, i.e.
(lightness) > 50, pH < 6.0 and drip loss > 5.0%, nor were these PSE-related variables influenced by the dietary treatment. Contents of crude protein and fat in LM did not differ between the two dietary groups. In conclusion, the present results suggest that growth performance and the occurrence of the PSE or 'watery' carcass are not influenced significantly by increasing the plane of nutrition in finishing pigs when the finisher is adequate nutritionally for supporting maximal lean growth. However, increasing the plane of nutrition is likely to result in an increase in fat deposition.
Comparison of Meat Color and Nutritional Composition of m. longissimus lumborum from Domestic Fed Hanwoo, Holstein and Imported Angus Steers
Oh, Mi-Ra ; Park, Beom-Young ; Seong, Pil-Nam ; Cho, Soohyun ; Kang, Keun-Ho ; Kim, Jin-Hyung ; Jeong, Seok-Geun ; Lee, Jun-Soo ; Jeong, Dawoon ;
Journal of Animal Science and Technology, volume 54, issue 6, 2012, Pages 455~462
DOI : 10.5187/JAST.2012.54.6.455
This study was conducted to investigate the carcass characteristics and nutritional composition of longissimus lumborum (LL) muscle of domestic steer beef (Hanwoo and Holstein born and raised in Korea) and imported beef (Angus imported from Australia, raised for about 6 months in Korea). A total of twelve steers (4 steers per each breed and quality grade 2) were slaughtered, LL muscles were separated and stored at
for 7 days. The pH values of Holstein meat were notably higher than those of Hanwoo, and WHC values were similar between Hanwoo and Holstein, but Angus showed some differences. The WHC of the LL muscle from Hanwoo was better than those from Holstein and Angus when stored for fourteen days. The CIE
values of the LL muscle from Hanwoo were significantly higher than those of Angus at day 7 (p<0.05). Oxymyoglobin and metmyoglobin contents were not significantly different among three breeds at days 7 and 14. In the intra-muscular fat contents, the LL muscle of Hanwoo was significantly higher than Holstein, whereas the moisture and ash contents tended to be lower than the others. In mineral contents, P and Zn contents in the LL muscle of Holstein were higher than the other breeds, and K contents was higher in Hanwoo and Holstein meat than Angus.
Selection and Classification of Bacterial Strains Using Standardization and Cluster Analysis
Lee, Sang Moo ; Kim, Kyoung Hoon ; Kim, Eun Joong ;
Journal of Animal Science and Technology, volume 54, issue 6, 2012, Pages 463~469
DOI : 10.5187/JAST.2012.54.6.463
This study utilized a standardization and cluster analysis technique for the selection and classification of beneficial bacteria. A set of synthetic data consisting of 100 individual variables with three characteristics was created for analysis. The three characteristics assigned to each independent variable were designated to have different numeric scales, averages, and standard deviations. The variables were bacterial isolates at random, and the three characteristics were fermentation products, including cell yield, antioxidant activity of culture, and enzyme production. A standardization method utilizing a standard normal distribution equation to record fermentation yields of each isolate was employed to weight their different numeric scales and deviations. Following transformation, the data set was analyzed by cluster analysis. The Manhattan method for dissimilarity matrix construction along with complete linkage technique, an agglomerative method for hierarchical cluster analysis, was employed using statistical computing program R. A total of 100 isolates were classified into groups A, B, and C. In a comparison of the characteristics of each group, all characteristics in groups A and C were higher than those of group B. Isolates displaying higher cell yield were classified as group A, whereas those isolates showing high antioxidant activity and enzyme production were assigned to group C. The results of the cluster analysis can be useful for the classification of numerous isolates and the preparation of an isolation pool using numerical or statistical tools. The present study suggests that a simple technique can be applied to screen and select beneficial microbes using the freely downloadable statistical computing program R.