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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal DOI :
The Korean Society of Toxicology
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Volume & Issues
Volume 7, Issue 2 - Dec 1991
Volume 7, Issue 1 - Jun 1991
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PHENOTYPING OF PHENYTOIN TREATED RAT LIVER CYTOCHROME P-450 WITH MONOCLONAL ANTIBODIES
Jin, Hak-Song ; Won, Sun-Me ; Park, Sang-Shin ; Kim, Nak-Doo ;
Toxicological Research, volume 7, issue 1, 1991, Pages 1~12
The phenotyping of cytochrome P-450 in hepatic mivrosomes induced by phenytoin in the rats was carried out by using several monoclonal antibodies (MAbs) against specific P-450 isozymes. Phenytoin (180 mg/kg) was administered intrapritoneally for three consecutive days to the male Sprague-Dawley rats(100-120g). Solid phase radio-immunoassay showed higher binding affinity of MAb PB 2-66-3 and PCN 2-13-1 to the microsomes from phenytoin treated rats than those to from untreated rats, which was comparable to the level in phenobarbital induced rat hepatic microsomes.
A STUDY ON THE CYTOTOXIC EFFECTS OF MITOMYCIN C AND 5-FLUOROURACIL IN CULTURED RAT FIBROBLASTS
C. S. M ; Park, Hong-Seog ; Chung, Yeun-Tai ;
Toxicological Research, volume 7, issue 1, 1991, Pages 13~20
To investigate the cytotoxicity and genotoxicity of the DNA alkylating agnet, mitomycin C and the antimetabolite, 5-Fluorouracil (5-FU) in cultured rat fibroblasts, the colorimetric assay of netural red (NR) for cytotoxicity and for genotoxicity, sister chromatid exchange (SCE) assay and the measurement of the rate of DNA synthesis were performed in cells cultured in media containing various concentrations of mitomycin C and 5-FU. The uptake ability of neutral red decreased does-dependently. NR90 and NR50 values of mitomycin C were 1.49 nM and 6.87mM and 5-FU were 38.4mM AND 284.4Mm respectively.
SCREENING OF BENZODIAZEPINES IN URINE BY THE IMMUNOASSAY AND QUANTITATION BY GC-NPD METHOD
Park, Jongsei ; Park, Jeongeum ; Park, Myung-Ja ;
Toxicological Research, volume 7, issue 1, 1991, Pages 21~27
We developed a simple method to determine benzodiazepines in biological samples using electron capature detectors and nitrogen-phosphorous detectors. The extraction of 13 benezodiazepines in urine at pH 9.5 with toluene and its analysis in GC/NPD showed the peaks in 9-16 min. In this retention time range, the biological backaground was fairly low and the drugs could be identified in low concentrations. The benzodiazepines in urine samples were screened by the fluorescence polarization immunoassay and positive samples were confirmed by the GC/NPD method.
QUANTITATION OF BARBITURATES IN URINE BY GC/MS AND ITS COMPARISON TO FLUORESCENCE POLARIZATION IMMUNOASSAY
Choo, Hea-Young ; Park, Jeongeun ; Park, Myung-Ja ;
Toxicological Research, volume 7, issue 1, 1991, Pages 29~35
Barbiturates are commonly abused tranquilizer and a rapid method to determine these drugs in biological samples is needed. In this study, was screened barbiturates in urine specimens by the fluorescence polarization immunoassay method(FPIA) and the positive samples were confirmed and identified by the more definitive GC/MS method. Fifteen positive smples which have barbiturate values higher than 0.5 ng/ml were analyzed by the GC/MS method. Eight samples were identified as phenobarbital and five samples were identified as crotilbarbitone.
INFLUENCE OF OCHRATOXIN A-INDUCED NEPHROTOXICITY ON THE PHARMACOKINETICS OF GENTAMICIN IN RATS
Son, Deok-Soo ; Han, Sang-Seop ;
Toxicological Research, volume 7, issue 1, 1991, Pages 37~46
To evaluate the influence of ochratoxin A on the pharmarcokinetics of gentamicin, gentamicin concentrations in the serum, renal cortex and medulla together with parameters of the renal function and histological changes were compared between ochratoxin A-treated rats (0.1 mg of ochratoxin A/kg of body weight, ip, daily for 14 days) and normal rats. Gentamicin was given with a single intramuscular injection (10mg/kg of body weight). Ochratoxin A resulted in an increase of the half-life, the area under the concentration-time curve, the apparent volume of distribution and a decrease of the total body clearance of gentamicin, and accumulated significantly (p<0.01) more gentamicin in the kidneys.
실험동물 마우스의 혈액세포 분포에 관한 연구
Ian C. Guest ;
Toxicological Research, volume 7, issue 1, 1991, Pages 47~50
Hematological studies in two strains of normal laboratory mice, namely ICR and ddY which were produced in Koren were carried out. Blood analysis was performed by using different methods, a fully automated analyzer (ELT 1, 500), Coulter counter (model S plus IV) and manual technique to examine possible differences resulting from methodolgies'slight differences among the values of blood parameters were obtained. There was a slight difference noted between sexes, however, similar values were obtained from both strains.
랫트에 있어서 절수, 절식 및 절수 절식이 혈액학 및 혈액생화학적 PARAMETERS에 미치는 영향에 관한 연구
Toxicological Research, volume 7, issue 1, 1991, Pages 51~59
The purpose of the present study was to investigate the effects of non-watering, fasting and non-watering fasting for 16-17 hrs on haematological and biochemical parameters in SD rats. 1. Liver weight was decreased in the order of in the fasting group, non-watering fasting group and non-watering group in both sexes. 2. In fasting group and non-watering fasting group, haematologic parameters of male (HGB and MCH) and femal (MCHC) were trends to decrease compared with normal feeding & watering group. 3. In the differential leucocyte counts, there were no significant differences compared with noraml feeding % watering group.
Formaldehyde 가스 흡입에 의한 마우스의 급성독성 및 소핵 유발성에 관한 연구
Toxicological Research, volume 7, issue 1, 1991, Pages 61~71
The acute and genetic effect of formaldehyde on mice through inhalation route was studied. The Riley's chamber with one stack of cage was used for the exposure and the micronucleus test was performed under unprecedently maximum exposure concentration. LC50's of formaldehyde in mice by whole body exposure for 4 hours were 105.5 ppm with 95% confidence interval of 72.6 ppm and 143.2 ppm for male, and 159.2 ppm with 95% confidence interval of 116.5 ppm and 272.7 ppm for female. Cinicial symptoms by acute exposure were salivation, lacrimation, and abnormal respiration.
유기수은 유발 뇌손상에 미치는 셀레늄의 작용기전 및 전자현미경적 관찰
Jhoo, Wnag-Kee ; Kim, Hyoung-Chun ; Song, Ke-Yong ;
Toxicological Research, volume 7, issue 1, 1991, Pages 73~81
The present study was performed to explore the antioxidant effect of selenium on damaged brain induced by organic mercury. Male ICR mice were given consecutively 7 injections for 7 days of : (I) sodium selenite 1 mg/kg s.c. alone, (2) methylmercuric chloride 10mg/kg s.c. alone, (3) methylmercuric chloride simultaneously in combination with sodium selenite, and (4) saline alone as control respectively. Based on the above protocol, we monitored various oxyradical scavenging system as well as the finding of electron microscopy. The results from brain tissues were as follows` 1. Selenium inhibited the rate of generation of superoxide radical.
BrdU에 의한 DNA
Toxicological Research, volume 7, issue 1, 1991, Pages 83~92
Complexities of testis structure and function are emphasized in morphometrical and genotoxic evaluation by statistical analysis. F-344 rats were treated with azinphos methyl, cyclophosphomide, and dichlorvos. And Brdu was injected with intrapertionially before sacrifice. The existence and degree of DNA damage were measured by Brdu labeling index which represented relative amount of Brdu incorporated in DNA, morphometric change was evaluated by the relative length of tubular diameter in circular seminiferous tubules and the number of spermatogonia per Sertoli cell in stage IX seminiferous tubules.
STUDIES OF VARIOUS FACTORS ON INDUCTION OF EXPERMIMENTAL HEPATOCARCINOGENESIS IN SPRAUGE-DAWLEY RATS
Jeong, Ja-Young ; Kim, Hyoung-Chin ; Lee, Yong-Soon ;
Toxicological Research, volume 7, issue 1, 1991, Pages 93~112
This study was carried out to investigate the various factors on induction of experimental hepatocarcinogenesis in Sprauge-Dawley rats. The experimental animals were divided into three Experiment. Experiment I, II and III were began rats with initial age of 6, 16 and 55 weeks, respectively. All Experiment were injected intrapertioneal with diethylnitrosamine (DENA` 200 mg/kg) as an intiator and group 3, 4 of Experiment I, II and III were fed on diet containing 0.02% 2-acetylaminofluorene (2-AFF) as a promoter for 6 weeks. Three weeks after two-thirds partial hepatectomy was performed in group 2, 4 of Experiment I & II and group 3 of Experiment III.