Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal Basic Information
Journal DOI :
The Korean Society of Toxicology
Editor in Chief :
Volume & Issues
Volume 9, Issue 2 - Dec 1993
Volume 9, Issue 1 - Jun 1993
Selecting the target year
EFFECTS OF BHA AND ACETAMINOPHEN ON THE BILIARY EXCRETION OF PHENOLPHTHALEIN AND THE HEPATIC GLUCURONIDATION IN MALE RATS
Choe, Suck-Young ; Lim, Wha-Jae ; Rina Yu ;
Toxicological Research, volume 9, issue 2, 1993, Pages 133~145
The present study examined the effects of butylated hydroxyanisole (BHA) on acetaminophen (AA)-induced hepatotoxicity in male rats and also examined the effects of these compounds on the biliary excretion of phenolphthalein (PP) and the hepatic glucuronidation. Male Sprague-Da-wley rats were pretreated with BHA (0.75% in diet for 10 days) were given single dose of AA (600mg/kg, ip) and liver function was determined 24 hr later. Serum activity of alanine aminotransferase (ALT) and histopathology were used as indices of hepatotoxicity.
Xylazine과 그 대사산물의 조직내 분포
Toxicological Research, volume 9, issue 2, 1993, Pages 147~152
Xylazine has been used as tranquilizer for food producing animals. The concentration of xylazine and its metabolites in major organs are determined for the safety of meat. Xylazine was eliminated very rapidly as reported and not detected after 10 hrs in organs. However p-hydroxy-xylazine, a main metabolite of xylazine in urine, was detected in liver in the concentration of 0.37-2.58 mg/g tissue to 48 hrs. 2, 6-Dimethylisothiocyanate, another metabolite of xylazine and a possible toxicant, was detected in low concentration to 2 hrs in liver, kidney and muscle.
유기용매에 의한 CYP2E1의 유도발현 : 단백질형성 효율의 증가에 따른 조절규제기전
;Raymond F. Novak;
Toxicological Research, volume 9, issue 2, 1993, Pages 153~158
Pyridine and acetone are efficacious inducers of CYP2E1 in both rats and rabbits. The response in the elevation of CYP2E1 levels, changes in CYP2E1 mRNA levels, and enhanced translational processing of hepatic CYP2E1 mRNA during the early phase of CYP2E1 induction by the solvents pyridine and acetone were examined. Time-depen-dent incease in CYP2E1 levels occurred at early times (6-24h) following a single dose of pyridine treatmene, as assessed by Western immunoblot analysis, whereas the levels in CYP2E1 mRNA transiently decreased at 12h post-treatment, returning to the level present in untreated animals.
AMPLIFICATION OF MERCURY TOXICITY BY GLUTATHIONE DEPLETION IN V79 CELLS
Yisook Nam ; Chung, An-Sik ;
Toxicological Research, volume 9, issue 2, 1993, Pages 159~166
The treatmene of V79 cells with diethyl maleate (DEM) led to decrease in glutathione (GSH) level as increasing DEM concentration. Mercuric chloride, treated for 6 hrs with 2ng/ml, affected the GSH metabolizing enzymes glutathione S-transferase (GST) and glutathione peroxidase (GSP), dropping their activities to 60% and 75%, respectively, though not so much in GSH level(80%). However, the toxic effects of mercuric chloride on those enzymes and GSH level were both amplified when the Hg2+ treatment was combined with the preceding DEM treatment.
EFFECT OF DITHIOL MALONATE DERIVATIVES (DMDs) ON CARBON TETRACHLORIDE-INDUCED HEPATOTOXICITY IN PRIMARY CULTURES OF ADULT RAT HEPATOCYTES
Jung, Hyun-Ho ; Jeong, Tae-Cheon ; Yang, Kyu-Hwan ; Chun, Young-Jin ;
Toxicological Research, volume 9, issue 2, 1993, Pages 167~175
Protective effects of dithiol malonate derivatives (DMDs), YH-100, YH-150 and YH-439 on carbon tetrachloride-induced hepatotoxicity were investigated in primary rat hepatocytes culture. Treatment of DMDs to hepatocytes culture did not affect total cytochrome P-450 content and ECOD and AHH activities. Protein and RNA synthesis was also similar to control. Meanwhile, DMDs significantly decreased LDH release and in vitro lipid peroxidation induced by
. Accumulation of cellular triglyceride and decreased secretion of VLDL from liver cells by
treatment were also significantly protected.
EFFECTS OF TUMOR NECROSIS FACTOR-ALPHA ON CYTOCHROME P-450-DEPENDENT DRUG METABOLISM IN PRIMARY MOUSE HEPATOCYTES CULTURES AND MOUSE HEPATOMA CELLS
Jung, Hyun-Ho ; Jeong, Hye-Gwang ; Lee, Michael ;
Toxicological Research, volume 9, issue 2, 1993, Pages 177~186
Previous results from several laboratories have demonstrated that tumor necrosis factor-alpha (TNFalpha) depressed cytochrome P-450 (P-450)-dependent drug metabolism in vivo. However, there is some debate whether the action of TNFalpha is mediated by its direct effects on hepatocytes, or is indirectly mediated through the release of other mediators like IL-1 from macrophages. In the present studies, we investigated the effects of TNFalpha on P-450-dependent drug metabolizing enzyme as measured by 7-ethoxyresorufin O-deethylase (EROD) activity.
EFFECT OF PERTUSSIS TOXIN ON THE SECRETION OF
-ENKEPHALIN AND EXPRESSION OF PROENKEPHALIN A mRNA INDUCED BY NICOTINE, ANGIOTENSIN II, AND PHORBOL MYRISTATE ACETATE IN BOVINE ADRENAL MEDULLARY CHROMAFFIN CELLS
Hong W. Suh ; Kim, Yung H. ;
Toxicological Research, volume 9, issue 2, 1993, Pages 187~194
[Met5]-Enkephalin (ME) secretion and the expression of proenkephalin A (proENK) mRNA were studied following long-term exposure of bovine adrenal medullary chromaffin (BAMC) cells to pertussis toxin. Prolonged (24 hr) stimulation of BAMC cells with pertussis toxin increased the secretion of ME as well as proENK gene expression. BAMC cells were also incubated with pertussis toxin in the presence or absence of other secretagogues such as nicotine, angiotensin II and phorbol myristate acetate.
REGULATION OF PROENKEPHALIN GENE EXPRESSION AND MET-ENKEPHALIN SECRETION IN BOVINE ADRENAL MEDULLARY CHROMAFFIN CELLS AND C6 RAT GLIOMA CELLS
Suh, Hong-Won ;
Toxicological Research, volume 9, issue 2, 1993, Pages 195~206
The expression of proenkephalin (proENK) mRNA and Met-enkephalin (ME) secretion in C6 rat glioma cells and bovine adrenal medullary chromaffin (BAMC) cells were elucidated in the present study. The levels of proENK mRNA and ME secreted into the media in BAMC cells were measured in the presence of cycloheximide and 12-tetrade-canoylphorbol-13-acetate (TPA). Cycloheximide (20 nM) abolished the induction of proENK mRNA expression, protein synthesis and ME secretion by TPA (1nM), indicating that de novo protein synthesis was necessary for proENK gene expression and ME secretion.
배양된 해마 신경세포의 성장에 대한 납의 영향
Toxicological Research, volume 9, issue 2, 1993, Pages 207~215
Lead is an environmental toxicant that causes a marked deficit in cognative development in infants and children. Damage to the hippocampus has been linked to the lead-induced deficit in the learning process. The present study examined the effects of lead on the development of hippocampal neurons in vitro. Hippocampal neurons were incubated with various concentrations in lead acetate (1nM to 30 nM) for 72 hrs from 4 h after plating, and the percentage of living neurons bearing neurites, neurite outgrowth and migration of multipolar neurons in culture were determined.
흰쥐 태아 중뇌 배양세포에서 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine의 독성: 2',7',-Dichlorofluorescin diacetate를 이용한 연구
Toxicological Research, volume 9, issue 2, 1993, Pages 217~224
1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) is a well-known dopamine neuron-specific toxin. But the involvement of oxidative damage in the pathogenesis of MPTP-induced parkinsonism is still uncertain. In this study, by using 2',7',-dichlorofluorescin diacetate(DCFH-DA) that detects intracellular oxidative processes, the effect of MPTP on dichlorofluorescein fluorescence in dissociated cells from fetal rat mesencephalon in culture was investigated. At 7th day in culture, cells were loaded with DCFH-DA, and exposed to 1 mM MPTP or MPP+. MPTP induced dichlorofluorescein-fluorescence which was peaked at 3 min and mostly faded away 30 min after MPTP treatment.
Sci-B-Vac의 급성독성에 관한 연구
Toxicological Research, volume 9, issue 2, 1993, Pages 225~232
SCi-B-Vac, the 3rd hepatitis B vaccine , was selected for clinical evaluation on the basis of toxicologic profiles in preclinical studies. These studies were performed to obtain information on its toxic signs, organs which are mainly affected, and to estimate its lethality in mice and rats given Sci-B-Vac through two routes of administratin. In male and female rats given a single intragastrical dose of Sci-B-Vac, we estimated that
values were over 2.00 ml/100g B.W. (10ng/ml), respectively.
PATHOLOGICAL STUDIES ON THE CHRONIC TOXICITY OF METHAMPHETAMINE ADMINISTRATION
Rim, Byung-Moo ; Cho, Gye-Myung ; Chang, Jae-Hong ;
Toxicological Research, volume 9, issue 2, 1993, Pages 233~240
Toxic effects of chronic adminstration of methamphetamine (MA) to SD rats were studied in respect to histopathological changes induced in each organ. In experimental groups liver weight decreased and brain weights increased markedly compared with controls in the 12th month after subcutaneous injection of 0.5 mg and 5mg/kg/BW MA. Serum alkaline phosphotase levels increased, but marked decrease of cholesterol, triglyceride, and BUN levels were checked depending on both the dose of MA and duration of treatment.
CHANGES IN SUBPOPULATION OF BRONCHOALVEOLAR LAVAGE FLUID IN THE PULMONARY FIBROSIS INDUCED BY BLEOMYCIN OR PEPLOMYCIN
Kim, Dae-Joong ;
Toxicological Research, volume 9, issue 2, 1993, Pages 241~251
Present studies were carried out in order to estabilish the bronchoalveolar lavage method and to examine the response of bleomycin and peplomycin on the total cell number and the subpoulations of bronchoalveolar lavage fluid. A total of 24 male F344 rats, weighing 300-350 mg, were divided into 3 groups. Animals recelved either belomycin (BLM` 0.75 mg/0.2 ml/rat), peplomycin (PLM` 0.25mg/0.2ml/rat) for groups 2 and 3 or an equal volume of sterile saline lacking drugs for controls (group 1).
웅성 랫트에서 이염화메탄의 사염화 탄소 독성 증폭효과
Toxicological Research, volume 9, issue 2, 1993, Pages 253~262
The effects of dichloromethane (DCM) on carbon tetrachloride (CT) toxicity were examined in adult male rats. A concomitant treatment of rats with DCM (0.3, 0.6, 1.2 g/kg, po) significantly potentiated the hepatotoxicity of CT (1.0 g/kg, po) as determined by increase in serum GPT (glutamic pyruvic transaminase), GOT (glutamic oxaloacetic transaminase), and SDH (sorbitol dehydrogenase) activity 24 hr following the treatments. Serum LDH (lactate dehydrogenase) activity was increased by either DCM or CT treatment.