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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Asian-Australasian Journal of Animal Sciences
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Journal DOI :
Asian Australasian Association of Animal Production Societies
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Volume & Issues
Volume 28, Issue 12 - Dec 2015
Volume 28, Issue 11 - Nov 2015
Volume 28, Issue 10 - Oct 2015
Volume 28, Issue 9 - Sep 2015
Volume 28, Issue 8 - Aug 2015
Volume 28, Issue 7 - Jul 2015
Volume 28, Issue 6 - Jun 2015
Volume 28, Issue 5 - May 2015
Volume 28, Issue 4 - Apr 2015
Volume 28, Issue 3 - Mar 2015
Volume 28, Issue 2 - Feb 2015
Volume 28, Issue 1 - Jan 2015
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A Genetic Analysis of Taoyuan Pig and Its Phylogenetic Relationship to Eurasian Pig Breeds
Li, Kuan-Yi ; Li, Kuang-Ti ; Cheng, Chun-Chun ; Chen, Chia-Hsuan ; Hung, Chien-Yi ; Ju, Yu-Ten ;
Asian-Australasian Journal of Animal Sciences, volume 28, issue 4, 2015, Pages 457~466
DOI : 10.5713/ajas.14.0595
Taoyuan pig is a native Taiwan breed. According to the historical record, the breed was first introduced to Taiwan from Guangdong province, Southern China, around 1877. The breed played an important role in Taiwan`s early swine industry. It was classified as an indigenous breed in 1986. After 1987, a conserved population of Taoyuan pig was collected and reared in isolation. In this study, mitochondrial DNA sequences and 18 microsatellite markers were used to investigate maternal lineage and genetic diversity within the Taoyuan pig population. Population differentiation among Taoyuan, Asian type, and European type pig breeds was also evaluated using differentiation indices. Only one D-loop haplotype of the Taoyuan pig was found. It clustered with Lower Changjiang River Basin and Central China Type pig breeds. Based on the polymorphism of microsatellite markers, a positive fixation index value (
) indicates that the conserved Taoyuan population suffers from inbreeding. In addition, high
values (>0.2105) were obtained, revealing high differentiation among these breeds. Non-metric multi-dimensional scaling showed a clear geometric structure among 7 breeds. Together these results indicate that maternally Taoyuan pig originated in the Lower Changjiang River Basin and Central China; however, since being introduced to Taiwan differentiation has occurred. In addition, Taoyuan pig has lost genetic diversity in both its mitochondrial and nuclear genomes.
Polymorphisms in Epigenetic and Meat Quality Related Genes in Fourteen Cattle Breeds and Association with Beef Quality and Carcass Traits
Liu, Xuan ; Usman, Tahir ; Wang, Yachun ; Wang, Zezhao ; Xu, Xianzhou ; Wu, Meng ; Zhang, Yi ; Zhang, Xu ; Li, Qiang ; Liu, Lin ; Shi, Wanhai ; Qin, Chunhua ; Geng, Fanjun ; Wang, Congyong ; Tan, Rui ; Huang, Xixia ; Liu, Airong ; Wu, Hongjun ; Tan, Shixin ; Yu, Ying ;
Asian-Australasian Journal of Animal Sciences, volume 28, issue 4, 2015, Pages 467~475
DOI : 10.5713/ajas.13.0837
Improvement for carcass traits related to beef quality is the key concern in beef production. Recent reports found that epigenetics mediates the interaction of individuals with environment and nutrition. The present study was designed to analyze the genetic effect of single nucleotide polymorphisms (SNPs) in seven epigenetic-related genes (DNMT1, DNMT3a, DNMT3b, DNMT3L, Ago1, Ago2, and HDAC5) and two meat quality candidate genes (CAPN1 and PRKAG3) on fourteen carcass traits related to beef quality in a Snow Dragon beef population, and also to identify SNPs in a total of fourteen cattle populations. Sixteen SNPs were identified and genotyped in 383 individuals sampled from the 14 cattle breeds, which included 147 samples from the Snow Dragon beef population. Data analysis showed significant association of 8 SNPs within 4 genes related to carcass and/or meat quality traits in the beef populations. SNP1 (13154420A>G) in exon 17 of DNMT1 was significantly associated with rib-eye width and lean meat color score (p<0.05). A novel SNP (SNP4, 76198537A>G) of DNMT3a was significantly associated with six beef quality traits. Those individuals with the wild-type genotype AA of DNMT3a showed an increase in carcass weight, chilled carcass weight, flank thicknesses, chuck short rib thickness, chuck short rib score and in chuck flap weight in contrast to the GG genotype. Five out of six SNPs in DNMT3b gene were significantly associated with three beef quality traits. SNP15 (45219258C>T) in CAPN1 was significantly associated with chuck short rib thickness and lean meat color score (p<0.05). The significant effect of SNP15 on lean meat color score individually and in combination with each of other 14 SNPs qualify this SNP to be used as potential marker for improving the trait. In addition, the frequencies of most wild-type alleles were higher than those of the mutant alleles in the native and foreign cattle breeds. Seven SNPs were identified in the epigenetic-related genes. The SNP15 in CAPN1 could be used as a powerful genetic marker in selection programs for beef quality improvement in the Snow Dragon Beef population.
Genetic Parameters for Linear Type Traits and Milk, Fat, and Protein Production in Holstein Cows in Brazil
Campos, Rafael Viegas ; Cobuci, Jaime Araujo ; Kern, Elisandra Lurdes ; Costa, Claudio Napolis ; McManus, Concepta Margaret ;
Asian-Australasian Journal of Animal Sciences, volume 28, issue 4, 2015, Pages 476~484
DOI : 10.5713/ajas.14.0288
The objective of this study was to estimate genetic and phenotypic parameters for linear type traits, as well as milk yield (MY), fat yield (FY) and protein yield (PY) in 18,831 Holstein cows reared in 495 herds in Brazil. Restricted maximum likelihood with a bivariate model was used for estimation genetic parameters, including fixed effects of herd-year of classification, period of classification, classifier and stage of lactation for linear type traits and herd-year of calving, season of calving and lactation order effects for production traits. The age of cow at calving was fitted as a covariate (with linear and quadratic terms), common to both models. Heritability estimates varied from 0.09 to 0.38 for linear type traits and from 0.17 to 0.24 for production traits, indicating sufficient genetic variability to achieve genetic gain through selection. In general, estimates of genetic correlations between type and production traits were low, except for udder texture and angularity that showed positive genetic correlations (>0.29) with MY, FY, and PY. Udder depth had the highest negative genetic correlation (-0.30) with production traits. Selection for final score, commonly used by farmers as a practical selection tool to improve type traits, does not lead to significant improvements in production traits, thus the use of selection indices that consider both sets of traits (production and type) seems to be the most adequate to carry out genetic selection of animals in the Brazilian herd.
Rumen Degradability and Post-ruminal Digestion of Dry Matter, Nitrogen and Amino Acids of Three Protein Supplements
Gao, Wei ; Chen, Aodong ; Zhang, Bowen ; Kong, Ping ; Liu, Chenli ; Zhao, Jie ;
Asian-Australasian Journal of Animal Sciences, volume 28, issue 4, 2015, Pages 485~493
DOI : 10.5713/ajas.14.0572
This study evaluated the in situ ruminal degradability, and subsequent small intestinal digestibility (SID) of dry matter, crude protein (CP), and amino acids (AA) of cottonseed meal (CSM), sunflower seed meal (SFSM) and distillers dried grains with solubles (DDGS) by using the modified three-step in vitro procedure. The ruminal degradability and subsequent SID of AA in rumen-undegradable protein (RUP-AA) varied among three protein supplements. The result show that the effective degradability of DM for SFSM, CSM, and DDGS was 60.8%, 56.4%, and 41.0% and their ruminal fermentable organic matter was 60.0%, 55.9%, and 39.9%, respectively. The ruminal degradable protein (RDP) content in CP for SFSM, CSM, and DDGS was 68.3%, 39.0%, and 32.9%, respectively, at the ruminal solid passage rate of 1.84%/h. The SFSM is a good source of RDP for rumen micro-organisms; however, the SID of RUP of SFSM was lower. The DDGS and CSM are good sources of RUP for lambs to digest in the small intestine to complement ruminal microbial AA of growing lambs. Individual RUP-AA from each protein source was selectively removed by the rumen microorganisms, especially for Trp, Arg, His, and Lys (p<0.01). The SID of individual RUP-AA was different within specific RUP origin (p<0.01). Limiting amino acid was Leu for RUP of CSM and Lys for both RUP of SFSM and DDGS, respectively. Therefore, different protein supplements with specific limitations should be selected and combined carefully in growing lambs ration to optimize AA balance.
Effects of Supplementation of Mulberry (Morus alba) Foliage and Urea-rice Bran as Fermentable Energy and Protein Sources in Sheep Fed Urea-treated Rice Straw Based Diet
Yulistiani, Dwi ; Jelan, Z.A. ; Liang, J.B. ; Yaakub, H. ; Abdullah, N. ;
Asian-Australasian Journal of Animal Sciences, volume 28, issue 4, 2015, Pages 494~501
DOI : 10.5713/ajas.14.0406
A digestibility study was conducted to evaluate the effects of supplementing mulberry foliage and urea rice-bran as a source of fermentable energy and protein to 12 sheep fed diets based on urea-treated rice straw (TRS). The three dietary treatments were: T1, TRS with mulberry; T2, TRS with 50% mulberry replaced with rice bran and urea; and T3, TRS with rice bran and urea. The study was arranged in a completely randomized design with four replications for each treatment. The sheep were fed one of the three diets and the supplements were offered at 1.2% of the body weight (BW) and the TRS was provided ad libitum. There were no differences (p>0.05) among the three treatment groups with respect to dry matter (DM) intake (
) and DM, organic matter (OM), and crude protein (CP) digestibility (
respectively for DM, OM, and CP). The digestibility of fiber (neutral detergent fiber [NDF] and acid detergent fiber) was significantly lower (p<0.05) for T3 (46.2 and 46.6 respectively) compared to T1 (55.8 and 53.7 respectively) and T2 (54.1 and 52.8 respectively). Nitrogen (N) intake by sheep on diet T3 was significantly (p<0.05) higher than sheep fed diet T1. However, N balance did not differ among the three diets (
). In contrast, the rumen ammonia (
) concentrations in sheep fed T2 and T3 were significantly (p<0.05) higher than in sheep fed T1. The
concentrations for all three diets were above the critical value required for optimum rumen microbial growth and synthesis. Total volatile fatty acid concentrations were highest (p<0.05) in T1 (120.3 mM), whilst the molar proportion of propionic acid was highest in T3 (36.9%). However, the microbial N supply in sheep fed T1 and T3 was similar but was significantly (p<0.05) higher than for sheep fed T2. It was concluded that mulberry foliage is a potential supplement of fermentable energy and protein for sheep fed TRS based diet. The suggested level of supplementation is 1.2% of BW or 32% of the total diet since it resulted in similar effects on the intake of DM, OM, and NDF, digestibility of DM, OM, and CP, N utilization and microbial supply when compared to rice bran and urea supplementation.
Fermentation Characteristics and Lactic Acid Bacteria Succession of Total Mixed Ration Silages Formulated with Peach Pomace
Hu, Xiaodong ; Hao, Wei ; Wang, Huili ; Ning, Tingting ; Zheng, Mingli ; Xu, Chuncheng ;
Asian-Australasian Journal of Animal Sciences, volume 28, issue 4, 2015, Pages 502~510
DOI : 10.5713/ajas.14.0508
The objective of this study was to assess the use of peach pomace in total mixed ration (TMR) silages and clarify the differences in aerobic stability between TMR and TMR silages caused by lactic acid bacteria (LAB). The TMR were prepared using peach pomace, alfalfa hay or Leymus chinensis hay, maize meal, soybean meal, cotton meal, limestone, a vitamin-mineral supplement, and salt in a ratio of 6.0:34.0:44.4:7.0:5.0:2.5:1.0:0.1 on a dry matter (DM) basis. Fermentation quality, microbial composition, and the predominant LAB were examined during ensiling and aerobic deterioration. The results indicated that the TMR silages with peach pomace were well fermented, with low pH and high lactic acid concentrations. The aerobic stability of TMR silages were significantly higher than that of TMR. Compared with TMR silages with alfalfa hay, TMR silage with Leymus chinensis hay was much more prone to deterioration. Although the dominant LAB were not identical in TMR, the same dominant species, Lactobacillus buchneri and Pediococcus acidilactici, were found in both types of TMR silages after 56 d of ensiling, and they may play an important role in the aerobic stability of TMR silages.
Fermentation Characteristics and Microbial Diversity of Tropical Grass-legumes Silages
Ridwan, Roni ; Rusmana, Iman ; Widyastuti, Yantyati ; Wiryawan, Komang G. ; Prasetya, Bambang ; Sakamoto, Mitsuo ; Ohkuma, Moriya ;
Asian-Australasian Journal of Animal Sciences, volume 28, issue 4, 2015, Pages 511~518
DOI : 10.5713/ajas.14.0622
Calliandra calothyrsus preserved in silage is an alternative method for improving the crude protein content of feeds for sustainable ruminant production. The aim of this research was to evaluate the quality of silage which contained different levels of C. calothyrsus by examining the fermentation characteristics and microbial diversity. Silage was made in a completely randomized design consisting of five treatments with three replications i.e.: R0, Pennisetum purpureum 100%; R1, P. purpureum 75%+C. calothyrsus 25%;, R2, P. purpureum 50%+C. calothyrsus 50%; R3, P. purpureum 25%+C. calothyrsus 75%; and R4, C. calothyrsus 100%. All silages were prepared using plastic jar silos (600 g) and incubated at room temperature for 30 days. Silages were analyzed for fermentation characteristics and microbial diversity. Increased levels of C. calothyrsus in silage had a significant effect (p<0.01) on the fermentation characteristics. The microbial diversity index decreased and activity was inhibited with increasing levels of C. calothyrsus. The microbial community indicated that there was a population of Lactobacillus plantarum, L. casei, L. brevis, Lactococcus lactis, Chryseobacterium sp., and uncultured bacteria. The result confirmed that silage with a combination of grass and C. calothyrsus had good fermentation characteristics and microbial communities were dominated by L. plantarum.
Milk Production and Income over Feed Costs in Dairy Cows Fed Medium-roasted Soybean Meal and Corn Dried Distiller`s Grains with Solubles
Thanh, Lam Phuoc ; Suksombat, Wisitiporn ;
Asian-Australasian Journal of Animal Sciences, volume 28, issue 4, 2015, Pages 519~529
DOI : 10.5713/ajas.14.0685
The aims of this study were to determine the effects of feeding medium-roasted soybean meal (SBM) and corn dried distiller`s grains with solubles (CDDGS) in dairy cows on milk production and income over feed costs. A randomized complete block design experiment was conducted with 24 crossbred multiparous Holstein Friesian dairy cows in early- and mid-lactation. Four dietary treatments were as follows: basal diet without feed substitute (Control), 7.17% dry matter (DM) roasted SBM replaced for concentrate (R-SBM), 11.50% DM CDDGS replaced for concentrate (DDGS), and 3.58% DM roasted SBM plus 5.75% DM CDDGS replaced for concentrate (SB-DG). The roasted SBM was produced using a medium-heated treatment at
for 180 min. Dry matter intake was not affected by feeding high rumen undegradable protein (RUP) sources, but the replacement of roasted SBM and CDDGS for concentrate significantly improved (p<0.001) RUP intake (0.90, 0.86, and 0.88 kg/d corresponding to R-SBM, DDGS, and SB-DG) compared to the control (0.61 kg/d). Feeding roasted SBM and CDDGS alone or in combination had no significant effect on milk composition of dairy cows (p>0.05), whereas milk yield was significantly increased by 3.08 kg/d in the SB-DG group relative to the control group (p<0.01). Net income was meaningfully increased (p<0.05) from 4th week post feeding, the SB-DG group reached the greatest net income ($3.48/head/d) while the control group had the lowest value ($2.60/head/d). In conclusion, the use of CDDGS alone or in combination with medium-roasted SBM as substitute for concentrate in lactating dairy cattle diet led to improved milk production and net income over feed costs without affecting total dry matter intake and milk composition, while feeding medium-roasted SBM seemed to show intermediate values in almost parameters.
Effects of Flavonoid-rich Plant Extracts on In vitro Ruminal Methanogenesis, Microbial Populations and Fermentation Characteristics
Kim, Eun T. ; Guan, Le Luo ; Lee, Shin J. ; Lee, Sang M. ; Lee, Sang S. ; Lee, Il D. ; Lee, Su K. ; Lee, Sung S. ;
Asian-Australasian Journal of Animal Sciences, volume 28, issue 4, 2015, Pages 530~537
DOI : 10.5713/ajas.14.0692
The objective of this study was to evaluate the in vitro effects of flavonoid-rich plant extracts (PE) on ruminal fermentation characteristics and methane emission by studying their effectiveness for methanogenesis in the rumen. A fistulated Holstein cow was used as a donor of rumen fluid. The PE (Punica granatum, Betula schmidtii, Ginkgo biloba, Camellia japonica, and Cudrania tricuspidata) known to have high concentrations of flavonoid were added to an in vitro fermentation incubated with rumen fluid. Total gas production and microbial growth with all PE was higher than that of the control at 24 h incubation, while the methane emission was significantly lower (p<0.05) than that of the control. The decrease in methane accumulation relative to the control was 47.6%, 39.6%, 46.7%, 47.9%, and 48.8% for Punica, Betula, Ginkgo, Camellia, and Cudrania treatments, respectively. Ciliate populations were reduced by more than 60% in flavonoid-rich PE treatments. The Fibrobacter succinogenes diversity in all added flavonoid-rich PE was shown to increase, while the Ruminoccocus albus and R. flavefaciens populations in all PE decreased as compared with the control. In particular, the F. succinogenes community with the addition of Birch extract increased to a greater extent than that of others. In conclusion, the results of this study showed that flavonoid-rich PE decreased ruminal methane emission without adversely affecting ruminal fermentation characteristics in vitro in 24 h incubation time, suggesting that the flavonoid-rich PE have potential possibility as bio-active regulator for ruminants.
Effect of Olive Leaf (Olea europaea) Powder on Laying Hens Performance, Egg Quality and Egg Yolk Cholesterol Levels
Cayan, Huseyin ; Erener, G. ;
Asian-Australasian Journal of Animal Sciences, volume 28, issue 4, 2015, Pages 538~543
DOI : 10.5713/ajas.14.0369
This experiment was conducted to measure the effects of olive leaf powder on performance, egg yield, egg quality and yolk cholesterol level of laying hens. A total of 120 Lohmann Brown laying hens of 22 weeks old were used in this experiment. The birds were fed on standard layer diets containing 0, 1%, 2%, or 3% olive leaf powder for 8 weeks. Egg weight and yield were recorded daily; feed intake weekly; egg quality and cholesterol content at the end of the trial. Olive leaf powder had no effect on feed intake, egg weight, egg yield and feed conversion ratio (p>0.05) while olive leaf powder increased final body weight of hens (p<0.05). Dietary olive leaf powder increased yellowness in yolk color (p<0.01) without affecting other quality parameters. Yolk cholesterol content was tended to decrease about 10% (p>0.05). To conclude, olive leaf powder can be used for reducing egg yolk cholesterol content and egg yolk coloring agent in layer diets.
Effects of Heat-oxidized Soy Protein Isolate on Growth Performance and Digestive Function of Broiler Chickens at Early Age
Chen, X. ; Chen, Y.P. ; Wu, D.W. ; Wen, C. ; Zhou, Y.M. ;
Asian-Australasian Journal of Animal Sciences, volume 28, issue 4, 2015, Pages 544~550
DOI : 10.5713/ajas.14.0609
This study was conducted to investigate effects of heat-oxidized soy protein isolate (HSPI) on growth performance, serum biochemical indices, apparent nutrient digestibility and digestive function of broiler chickens. A total of 320 1-day-old Arbor Acres chicks were randomly divided into 4 groups with 8 replicates of 10 birds, receiving diets containing soy protein isolate (SPI, control group) or the same amount of SPI heated in an oven at
for 1, 4, or 8 h, for 21 days, respectively. The results indicated that compared with the control group, body weight gain and feed intake of birds fed diet containing SPI heated for 8 h were significantly lower (p<0.05). Serum urea nitrogen concentration was higher in the broilers fed diet containing SPI heated for 4 or 8 h at d 21 (p<0.05). In contrast, serum glucose content was decreased by HSPI substitution at d 21 (p<0.05). The relative pancreas weight in HSPI groups was higher than that in the control group at d 21 (p<0.05). Meanwhile, the opposite effect was observed for relative weight of anterior intestine and ileum in broilers fed a diet containing SPI heated for 8 h (p<0.05). Birds fed diets containing SPI heated for 4 or 8 h had a decreased lipase activity in anterior intestinal content at d 14 and 21 (p<0.05), respectively. In addition, the same effect was also noted in broilers given diets containing SPI heated for 1 h at d 21 (p<0.05). Similarly, amylase, protease and trypsin activity in anterior intestinal content were lower in broilers fed diets containing SPI heated for 8 h at d 21 (p<0.05). The apparent digestibility of dry matter (DM) from d 8 to 10 and DM, crude protein (CP), and ether extract from d 15 to 17 were lower in broilers fed diets containing SPI heated for 8 h (p<0.05). Besides, birds given diets containing SPI heated for 4 h also exhibited lower CP apparent digestibility from d 15 to 17 (p<0.05). It was concluded that HSPI inclusion can exert a negative influence on the growth performance of broilers, which was likely to result from the simultaneously compromised digestive function.
Evaluation of Optimum Dietary Threonine Requirement by Plasma Free Threonine and Ammonia Concentrations in Surgically Modified Rainbow Trout, Oncorhynchus mykiss
Yun, Hyeonho ; Park, Gunjun ; Ok, Imho ; Katya, Kumar ; Heung, Silas ; Bai, Sungchul C. ;
Asian-Australasian Journal of Animal Sciences, volume 28, issue 4, 2015, Pages 551~558
DOI : 10.5713/ajas.14.0495
This study was carried out to evaluate the dietary threonine requirement by measuring the plasma free threonine and ammonia concentrations in rainbow trout, Oncorhynchus mykiss after dorsal aorta cannulation. A total of 70 fish (average initial weight
) were randomly distributed into each of the 14 net cages (5 fish/cage). After 48 hours (h) of feed deprivation, each group was intubated at 1% body weight with one of the seven L-amino acid based diets containing graded levels of threonine (0.42%, 0.72%, 0.92%, 1.12%, 1.32%, 1.52%, or 1.82% of diet, dry matter basis). Blood samples were taken at 0, 5, and 24 h after intubation. Post-prandial plasma free threonine concentrations (PPthr) of fish 5 h after intubation with diets containing 1.32% or more threonine were significantly higher than those of fish intubated with diets containing 1.12% or less threonine (p<0.05). Post-absorptive free threonine concentrations (PAthr) after 24 h of intubation of the fish with diets containing 0.92% or more threonine were significantly higher than those of fish intubated with diets containing 0.72% or less threonine. Post-prandial plasma ammonia concentrations (PPA, 5 h after intubation) were not significantly different among fish intubated with diets containing 1.12% or less threonine, except the PPA of fish intubated with diet containing 0.42% threonine. Broken-line model analyses of PPthr, PAthr, and PPA indicated that the dietary threonine requirement of rainbow trout should be between 0.95% (2.71) and 1.07% (3.06) of diet (% of dietary protein on a dry matter basis).
Ex vivo Digestion of Milk from Red Chittagong Cattle Focusing Proteolysis and Lipolysis
Islam, Mohammad Ashiqul ; Ekeberg, Dag ; Rukke, Elling-Olav ; Vegarud, Gerd Elisabeth ;
Asian-Australasian Journal of Animal Sciences, volume 28, issue 4, 2015, Pages 559~567
DOI : 10.5713/ajas.14.0269
Ex vivo digestion of proteins and fat in Red Chittagong Cattle milk from Bangladesh was carried out using human gastrointestinal enzymes. This was done to investigate the protein digestion in this bovine breed`s milk with an especial focus on the degradation of the allergenic milk proteins;
-lactoglobulin and also to record the generation of peptides. Lipolysis of the milk fat and release of fatty acids were also under consideration. After 40 min of gastric digestion, all the
-caseins were digested completely while
-lactoglobulin remained intact. During 120 min of duodenal digestion
-lactoglobulin was reduced, however, still some intact
-lactoglobulin was observed. The highest number of peptides was identified from
-casein and almost all the peptides from
-lactoglobulin were identified from the gastric and duodenal samples, respectively. No lipolysis was observed in the gastric phase of digestion. After 120 min of duodenal digestion, milk fat showed 48% lipolysis. Medium (C10:0 to C16:0) and long (
) chain fatty acids showed 6% to 19% less lipolysis than the short (C6:0 to C8:0) chain fatty acids. Among the unsaturated fatty acids
showed highest lipolysis (81%) which was more than three times of
and all other unsaturated fatty acids showed lipolysis ranging from 32% to 38%. The overall digestion of Bangladeshi Red Cattle milk was more or less similar to the digestion of Nordic bovine milk (Norwegian Red Cattle).
Rapid Origin Determination of the Northern Mauxia Shrimp (Acetes chinensis) Based on Allele Specific Polymerase Chain Reaction of Partial Mitochondrial 16S rRNA Gene
Kang, Jung-Ha ; Noh, Eun-Soo ; Park, Jung-Youn ; An, Chel-Min ; Choi, Jung-Hwa ; Kim, Jin-Koo ;
Asian-Australasian Journal of Animal Sciences, volume 28, issue 4, 2015, Pages 568~572
DOI : 10.5713/ajas.14.0613
Acetes chinensis is an economically important shrimp that belongs to the Sergestidae family; following fermentation, A. chinensis` economic value, however, is low in China, and much of the catch in China is exported to Korea at a low price, thus leading to potential false labeling. For this reason, we developed a simple method to identify A. chinensis` origin using allele-specific polymerase chain reaction (PCR). Ten single nucleotide polymorphisms (SNPs) were identified from partial (i.e., 570 bp) DNA sequence analysis of the mitochondrial 16s rRNA gene in 96 Korean and 96 Chinese individual shrimp. Among 10 SNP sites, four sites were observed in populations from both countries, and two sites located in the middle with SNP sites at their 3`-ends were used to design allele-specific primers. Among the eight internal primers, the C220F primer specific to the Chinese A. chinensis population amplified a DNA fragment of 364 bp only from that population. We were able to identify the A. chinensis population origin with 100% accuracy using multiplex PCR performed with two external primers and C220F primers. These results show that the 16S rRNA gene that is generally used for the identification of species can be used for the identification of the origin within species of A. chinensis, which is an important finding for the fair trade of the species between Korea and China.
Development and Characterization of a Novel Anti-idiotypic Monoclonal Antibody to Growth Hormone, Which Can Mimic Physiological Functions of Growth Hormone in Primary Porcine Hepatocytes
Lan, Hai-Nan ; Jiang, Hai-Long ; Li, Wei ; Wu, Tian-Cheng ; Hong, Pan ; Li, Yu Meng ; Zhang, Hui ; Cui, Huan-Zhong ; Zheng, Xin ;
Asian-Australasian Journal of Animal Sciences, volume 28, issue 4, 2015, Pages 573~583
DOI : 10.5713/ajas.14.0600
B-32 is one of a panel of monoclonal anti-idiotypic antibodies to growth hormone (GH) that we developed. To characterize and identify its potential role as a novel growth hormone receptor (GHR) agonist, we determined that B-32 behaved as a typical
based on a series of enzyme-linked immunosorbent assay assays. The results of fluorescence-activated cell sorting, indirect immunofluorescence and competitive receptor binding assays demonstrated that B-32 specifically binds to the GHR expressed on target cells. Next, we examined the resulting signal transduction pathways triggered by this antibody in primary porcine hepatocytes. We found that B-32 can activate the GHR and Janus kinase (2)/signal transducers and activators of transcription (JAK2/STAT5) signalling pathways. The phosphorylation kinetics of JAK2/STAT5 induced by either GH or B-32 were analysed in dose-response and time course experiments. In addition, B32 could also stimulate porcine hepatocytes to secrete insulin-like growth factors-1. Our work indicates that a monoclonal anti-idiotypic antibody to GH (B-32) can serve as a GHR agonist or GH mimic and has application potential in domestic animal (pig) production.
Characterization of the Fecal Microbial Communities of Duroc Pigs Using 16S rRNA Gene Pyrosequencing
Pajarillo, Edward Alain B. ; Chae, Jong Pyo ; Balolong, Marilen P. ; Kim, Hyeun Bum ; Seo, Kang-Seok ; Kang, Dae-Kyung ;
Asian-Australasian Journal of Animal Sciences, volume 28, issue 4, 2015, Pages 584~591
DOI : 10.5713/ajas.14.0651
This study characterized the fecal bacterial community structure and inter-individual variation in 30-week-old Duroc pigs, which are known for their excellent meat quality. Pyrosequencing of the V1-V3 hypervariable regions of the 16S rRNA genes generated 108,254 valid reads and 508 operational taxonomic units at a 95% identity cut-off (genus level). Bacterial diversity and species richness as measured by the Shannon diversity index were significantly greater than those reported previously using denaturation gradient gel electrophoresis; thus, this study provides substantial information related to both known bacteria and the untapped portion of unclassified bacteria in the population. The bacterial composition of Duroc pig fecal samples was investigated at the phylum, class, family, and genus levels. Firmicutes and Bacteroidetes predominated at the phylum level, while Clostridia and Bacteroidia were most abundant at the class level. This study also detected prominent inter-individual variation starting at the family level. Among the core microbiome, which was observed at the genus level, Prevotella was consistently dominant, as well as a bacterial phylotype related to Oscillibacter valericigenes, a valerate producer. This study found high bacterial diversity and compositional variation among individuals of the same breed line, as well as high abundance of unclassified bacterial phylotypes that may have important functions in the growth performance of Duroc pigs.
Stress Detection and Classification of Laying Hens by Sound Analysis
Lee, Jonguk ; Noh, Byeongjoon ; Jang, Suin ; Park, Daihee ; Chung, Yongwha ; Chang, Hong-Hee ;
Asian-Australasian Journal of Animal Sciences, volume 28, issue 4, 2015, Pages 592~598
DOI : 10.5713/ajas.14.0654
Stress adversely affects the wellbeing of commercial chickens, and comes with an economic cost to the industry that cannot be ignored. In this paper, we first develop an inexpensive and non-invasive, automatic online-monitoring prototype that uses sound data to notify producers of a stressful situation in a commercial poultry facility. The proposed system is structured hierarchically with three binary-classifier support vector machines. First, it selects an optimal acoustic feature subset from the sound emitted by the laying hens. The detection and classification module detects the stress from changes in the sound and classifies it into subsidiary sound types, such as physical stress from changes in temperature, and mental stress from fear. Finally, an experimental evaluation was performed using real sound data from an audio-surveillance system. The accuracy in detecting stress approached 96.2%, and the classification model was validated, confirming that the average classification accuracy was 96.7%, and that its recall and precision measures were satisfactory.
Reproduction Efficiency and Health Traits in Dorper, White Dorper, and Tsigai Sheep Breeds under Temperate European Conditions
Gavojdian, D. ; Budai, C. ; Cziszter, L.T. ; Csizmar, N. ; Javor, A. ; Kusza, S. ;
Asian-Australasian Journal of Animal Sciences, volume 28, issue 4, 2015, Pages 599~603
DOI : 10.5713/ajas.14.0659
The objective of the current pilot study was to evaluate the reproductive performance and health indicators in Dorper, White Dorper, and Tsigai breed ewes managed semi-intensively under European temperate conditions. A total of 544 ewe-year units were observed, with ewes (ranging from 1.5 to 8 years of age) managed under identical rearing conditions for a period of two consecutive production cycles (2012 through 2013 and 2013 through 2014). In general, significant (
) genotype-related disparities were found in occurrence rates for all health parameters taken into study. Clinical mastitis incidence was significantly lower (
) in Dorper (9.4%) and White Dorper (10.8%) breeds compared to that of Tsigai ewes (17.4%). Significant differences (
) for lameness were found between Dorper and Tsigai breeds, with occurrence rates of 8.0% and 2.9%, respectively. Incidence for pneumonia and abortion was not influenced (p>0.05) by the ewes` genotype. Litter size was significantly lower (
) in White Dorper breed than for Dorper and Tsigai ewes, of 1.21, 1.40, and 1.45, respectively. Conception rates and lambs survival were not affected (p>0.05) by genotype. Results suggest that South African Dorper and White Dorper sheep breeds have adapted well to the specific rearing conditions.