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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Preventive Nutrition and Food Science
Journal Basic Information
Journal DOI :
The Korean Society of Food Science and Nutrition
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Volume & Issues
Volume 15, Issue 4 - Dec 2010
Volume 15, Issue 3 - Sep 2010
Volume 15, Issue 2 - Jun 2010
Volume 15, Issue 1 - Mar 2010
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Suppressed Fat Accumulation in Rats Fed a Histidine-Enriched Diet
Endo, Michiko ; Kasaoka, Seiichi ; Takizawa, Miki ; Goto, Kiyoko ; Nakajima, Shigeru ; Moon, Soo-Kyung ; Kim, In-Soo ; Jeong, Bo-Young ; Nakamura, Soichiro ;
Preventive Nutrition and Food Science, volume 15, issue 1, 2010, Pages 1~6
DOI : 10.3746/jfn.2010.15.1.001
The effect on body fat accumulation on male Wistar rats undergoing continuous feeding with a histidine-enriched diet was investigated. Five-week-age rats were assigned to two groups and were fed either the control diet (purified diet AIN-
) or the histidine-enriched diet containing 3% histidine for 28 days. It was observed that both adipose tissue masses in retroperitoneal and epididymal areas of rats fed histidine-enriched diet significantly decreased (p<0.05) compared to those of control rats, while there was no significant difference in the food efficiency ratio between them. The blood levels of histidine derivatives of 3-methylhistidine and carnosine were significantly (p<0.05) increased in the rats fed a histidine-enriched diet, whereas there were no significant different between the histidine-enriched diet and control groups in the general amino acid distribution. Our results demonstrate that a histidine-enriched diet suppresses body fat accumulation in rats.
Protective Effects of Fermented Soymilk Extract on High Glucose-Induced Oxidative Stress in Human Umbilical Vein Endothelial Cells
Yi, Na-Ri ; Park, Min-Jung ; Han, Ji-Sook ;
Preventive Nutrition and Food Science, volume 15, issue 1, 2010, Pages 7~13
DOI : 10.3746/jfn.2010.15.1.007
We investigated whether the fermented soymilk extract (FSE) has protective effects against high glucose-induced oxidative stress in human umbilical vein endothelial cells (HUVECs). FSE was prepared via fermentation of soymilk with Bacillus subtilis followed by methanol extraction. To determine the protective effect of FSE, oxidative stress was induced by exposing of HUVECs to the high glucose (30 mM) for 48 hr. Exposure of HUVECs to high glucose for 48 hr resulted in a significant (p<0.05) decrease in cell viability, catalase, SOD and GSH-px activity and a significant (p<0.05) increase in intracellular ROS level and thiobarbituric acid reactive substances (TBARS) formation in comparison to the cells treated with 5.5 mM glucose. However, at concentration of 0.1 mg/mL, FSE treatment decreased intracellular ROS level and TBARS formation, and increased cell viability and activities of antioxidant enzymes including catalase, SOD and GSH-px in high glucose pretreated HUVEC. These results suggest that FSE may be able to protect HUVECs from high glucose-induced oxidative stress, partially through the antioxidative defense systems.
Anti-inflammatory Activity of Stevia rebaudiana in LPS-induced RAW 264.7 Cells
Jeong, Il-Yun ; Lee, Hyo-Jung ; Jin, Chang-Hyun ; Park, Yong-Dae ; Choi, Dae-Seong ; Kang, Min-Ah ;
Preventive Nutrition and Food Science, volume 15, issue 1, 2010, Pages 14~18
DOI : 10.3746/jfn.2010.15.1.014
Stevia rebaudiana (SR) is an herb used traditionally as a sweetener in Paraguay and Brazil, whose use is spreading to other countries, such as Japan, Korea and China. In addition to its low calorie sweet taste, SR appears to have other beneficial properties, such as hypotensive capabilities and inflammation reduction. To identify the bioactive natural constituents exerting anti-inflammatory activities, we examined the EtOAc fraction of SR. In the inflammatory mediator inhibitory assay from lipopolysaccharide (LPS)-activated macrophages, the EtOAc fraction significantly, and dose dependently, inhibited the enhanced production of nitric oxide (NO) and inducible nitric oxide synthase (iNOS) expression. We also found that treatment of cells with the EtOAc fraction significantly inhibited LPS-stimulated nuclear factor-
) reporter gene expression. Such inhibition of NF-
was closely associated with the inhibition of interleukin-6 (IL-6) and the monocyte chemoattractant protein-1 (MCP-1). Therefore, we suggest that SR has the potential for development as a functional food for the treatment of immune diseases, such as rheumatoid arthritis and lupus.
In vitro Effects of L-Ascorbic Acid and Acrylamide on Lymphocyte Proliferation in Young and Aged Mice
Kang, Nam-Sung ; Pyo, Suhk-Neung ; Sohn, Eun-Hwa ;
Preventive Nutrition and Food Science, volume 15, issue 1, 2010, Pages 19~23
DOI : 10.3746/jfn.2010.15.1.019
This study examined the effects of Acrylamide (ACR) and L-ascorbic acid (AsA) on the proliferation of splenocytes and the mitogen-stimulated lymphocyte proliferation in young (8 weeks) and aged (82 weeks) C57BL/6male mice in vitro. AsA increased splenocyte proliferation in both groups; however, this effect was higher in old mice, while the proliferation of lymphocyte was decreased except for treatment at
low concentration in both mice. In addition, ACR treatment resulted in decreased LPS-induced B lymphocyte proliferation and ConA-induced T lymphocyte proliferation in both groups. However, AsA increased LPS/ConA-induced lymphocyte proliferation in young groups and had no effects in old mice except at
Thus, the present data indicate that there is no difference effect of ACR and AsA on lymphocyte proliferation, whereas the effect of AsA on mitogen-induced cell proliferation was reduced in old mice. Overall, our results suggest that various immunomodulators have differing effects of lymphocytic proliferation on young versus aged mice.
Sodium and Potassium Balance and Their Relation to Nutrient Intakes in Young Adult Men and Women
Kim, Eun-Young ; Choi, Mi-Kyeong ;
Preventive Nutrition and Food Science, volume 15, issue 1, 2010, Pages 24~29
DOI : 10.3746/jfn.2010.15.1.024
This study was conducted to investigate sodium and potassium balances, as well as correlations among the relating factors in adult males and females. We collected blood, urine and feces samples as well as a dietary intake survey from 50 subjects. Then, we analyzed the sodium and potassium contents in blood, urine and feces, and evaluated their state of balance. The average ages of the study targets were 24.7 years old for males and 22.8 years old for females. The daily energy intake by the males was 1733.4 kcal and by the females was 1570.3 kcal. Sodium intakes were 138.3 mEq and 127.5 mEq for males and females, respectively. Potassium intakes were 43.1 mEq and 49.3 mEq, respectively. The daily excretions of sodium through urine were 136.6 mEq by males and 97.0 mEq by females and the excretions through feces were 2.2 mEq and 2.0 mEq, respectively. The daily excretions of potassium through urine were 20.2 mEq and 16.5 mEq by males and females respectively, and the excretions through feces were 7.7 mEq and 7.5 mEq male to female. The retention rates of sodium were 11.7% and 14.1% male to female, respectively, and the apparent absorption rates were 98.5% and 97.8%. Additionally, the retention rates of potassium were 32.9% and 39.8% and the apparent absorptions were 81.9% and 81.3%, both male and female. It was noted that, overall, the sodium intake of adult males and females is still higher than the recommended daily sodium intake, while the potassium intakes and excretions were found to be lower. Based on the results of this study, nutritional guidance and education is recommended to encourage decreased sodium intake and increased potassium intake, according to recommended standards.
Antioxidant and Anti-diabetes Activities of Methanolic Extract and Fractions of Astragalus membranaceus Roots
Park, Jae-Hyo ; Yin, Yu ; Wang, Myeong-Hyeon ;
Preventive Nutrition and Food Science, volume 15, issue 1, 2010, Pages 30~35
DOI : 10.3746/jfn.2010.15.1.030
The potential biological activities of methanol extract and 5 fractions (hexane,
, EtOAc, BuOH and water) from roots of Astragalus membranaceus were examined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, hydroxyl radical (
) scavenging activity, reducing power assays, lipid peroxidation inhibitory activity,
-glucosidase inhibition assays. The EtOAc fraction showed high DPPH free radical scavenging activity (
), hydroxyl radical scavenging activity (
), lipid peroxidation inhibitory activity (
) and a concentration dependence, with OD value ranging from 0.234 to 0.345 (0.1 to 0.5 mg/mL), for reducing power. The EtOAc fraction has the highest total phenolic content (
) and the
fraction has the highest flavonoid content (
). Meanwhile, hexane and EtOAc showed certain
-glucosidase inhibition activities. These results suggest that the methanol extract and fractions from Astragalus membranaceus root have significant antioxidant and anti-diabetes activities, which could be used as a potential source of pharmaceutical materials.
Phenolic Composition and Antioxidant Activities of Different Solvent Extracts from Pine Needles in Pinus Species
Kang, Yoon-Han ; Howard, Luke R. ;
Preventive Nutrition and Food Science, volume 15, issue 1, 2010, Pages 36~43
DOI : 10.3746/jfn.2010.15.1.036
The purpose of this study was to investigate the phenolic acid, proanthocyanidin (PAs), and flavonol glycoside contents, as well as the antioxidant activities of pine needle extracts from six species of young pine trees. The extracts were prepared from Section Pinus (Diploxylon): P. densiflora, P. sylvestris, P. pinaster and P. pinea, and Section Strobus (Haploxylon): P. koraiensis and P. strobus. Phenolics were extracted from pine needles with 80% acetone to obtain the soluble free fraction, and insoluble residues were digested with 4 M NaOH to obtain bound ethyl acetate and bound water fractions. Phenolics were analyzed by HPLC, and the hydrophilic antioxidant activity was measured using oxygen radical absorbance capacity (ORAC). Total phenolic and flavonoid contents of the soluble free fraction were higher than those of the bound ethyl acetate and bound water fractions. The main phenolics were monomers and polymers of PAs in the soluble free fraction, and phenolic acids and flavonol glycosides in bound ethyl acetate fraction. Flavonol glycosides found in different species of pine needles were qualitatively similar within fractions, but composition varied among Pinus sections. High levels of kaempferol arabinoside and an unknown compound were present in all Strobus species. The soluble free fraction had the highest antioxidant activity, followed by bound ethyl acetate and bound water fractions.
Antioxidant and Free Radical Scavenging Activity of Different Fractions from Hawthorn Fruit
Park, Jae-Hyo ; Li, Chunmei ; Hu, Weicheng ; Wang, Myeong-Hyeon ;
Preventive Nutrition and Food Science, volume 15, issue 1, 2010, Pages 44~50
DOI : 10.3746/jfn.2010.15.1.044
Hawthorn fruit is a conventional medicine used in treating cardiovascular diseases. Its therapeutic effects may relate to its antioxidant compounds. In this study, we evaluated the antioxidant activity of
, EtOAc, n-butanol and water fractions from 70% methanolic hawthorn fruit extract by total phenolic and flavonoid contents, total antioxidant activity, DPPH free radical scavenging activity, superoxide radical scavenging activity, reducing power assay, lipid peroxidation inhibitory activity and protective effect against hydroxyl-radical-induced DNA damage. Results showed that the EtOAc fraction contained significantly greater antioxidant activities than other fractions, which suggests that the potent EtOAc fraction should be used for further studies to identify the antioxidant compounds.
Antiradical Capacities of Perilla, Sesame and Sunflower Oil
Hong, Sun-Hee ; Kim, Mi-Jin ; Oh, Chan-Ho ; Yoon, Suk-Hoo ; Song, Yeong-Ok ;
Preventive Nutrition and Food Science, volume 15, issue 1, 2010, Pages 51~56
DOI : 10.3746/jfn.2010.15.1.051
The aim of this study is to examine the radical scavenging activity of perilla and sesame oil that Koreans traditionally consume. For DPPH radical scavenging activity, oil and its hexane/70% methanol extracts (ME) are used and for superoxide and hydroxyl radical scavenging activities, ME are used. Unrefined perilla oil, sesame oil, and refined sunflower oil are used. The yields for ME of perilla, sesame and sunflower oil are 0.57, 0.61, and 0.30%, respectively, and the amounts of phenolic compounds in ME of corresponding oil are 18.77, 88.64 and
tannic acid/mg, respectively.
for DPPH scavenging activity of perilla, sesame and sunflower oil are 2.12, 1.91, and 3.35 mg/mL, respectively and those for ME of corresponding oils are 0.42, 0.07, and 43.11 mg/mL, respectively. In DPPH assay, the solvent used for oil sample is iso-octane and that for ME is methanol. Superoxide anion scavenging activity of ME of perilla, sesame and sunflower oil tested at 1 mg/mL concentration are 21.10, 13.25, and 3.14%, respectively. Hydroxyl radical scavenging activities of those samples tested at 1 mg/mL concentration are 86.08, 93.30, and 93.17%, respectively. In summary, the refining process seems to remove the phenolic compound during oil processing. Antiradical substances in perilla and sesame oils responsible for scavenging DPPH radicals are present in the methanol fraction, while the antiradical substances in the sunflower oil are in the lipid fraction. DPPH scavenging activity of ME of sesame oil is significantly higher than that of perilla oil (p<0.05). However, superoxide anion scavenging capacity of ME of perilla oils was found to be greater than that of both sesame and sunflower oils (p<0.05).
Pectin from Passion Fruit Fiber and Its Modification by Pectinmethylesterase
Contreras-Esquivel, Juan Carlos ; Aguilar, Cristobal N. ; Montanez, Julio C. ; Brandelli, Adriano ; Espinoza-Perez, Judith D. ; Renard, Catherine M.G.C. ;
Preventive Nutrition and Food Science, volume 15, issue 1, 2010, Pages 57~66
DOI : 10.3746/jfn.2010.15.1.057
Passion fruit fiber pectin gels represent a new alternative pectin source with potential for food and non-food applications on a commercial scale. Pectic polysaccharides were extracted from passion fruit (Passiflora edulis) fiber using citric acid as a clean catalyst and autoclaved for 20 to 60 min at
. The best condition of pectin yield with the highest molecular weight was obtained with 1.0% of citric acid (250 mg/g dry passion fruit fiber pectin) for 20 min of autoclaving. Spectroscopic analyses by Fourier transform infrared, enzymatic degradation reactions, and ion-exchange chromatography assays showed that passion fruit pectin extracted for 20 min was homogeneous high methoxylated pectin (70%). Gel permeation analysis confirmed that the pectin extract obtained by autoclaving by 20 min showed higher molecular weights than those autoclaved for 40 and 60 min. Passion fruit pectin extracted for 20 min was enzymatically modified with fungal pectinmethylesterase to create restructured gels. Short autoclave treatment (20 min) with citric acid as extractant resulted in a significant increase of gel strength, improving pectin extraction in terms of functionality. The treatment of solubilized material (pectic polysaccharides) in the presence of insoluble material (cellulose and hemicellulose) with pectinmethylesterase and calcium led to the creation of a stiffer passion fruit fiber pectin gel, while syneresis was not observed.
Effects of Particle Size and Gelatinization of Job's Tears Powder on the Instant Properties
Han, Sung-Hee ; Park, Soo-Jea ; Lee, Seog-Won ; Rhee, Chul ;
Preventive Nutrition and Food Science, volume 15, issue 1, 2010, Pages 67~73
DOI : 10.3746/jfn.2010.15.1.067
The effects of particle sizes (small, medium and large sizes) and gelatinization treatment on the changes of the instant properties of Job's tears powder were investigated. The degree of gelatinization on the different particle size samples of Job's tears powder was the highest in the small particle size, and it also showed an increasing trend regardless of pregelatinizing whether it is or not as the particle size decreased from large particle size to small particle size. The water solubility index of the pregelatinized samples was high compared to that of ungelatinized samples regardless of particle size and temperatures. The water absorption and swelling power increased as particle size and temperature were increased. The dispersibility and sinkability of ungelatinized sample was increased as particle size and temperature were increased and it also showed lower value regardless of particle size and temperature. However, the dispersibility and sinkability of pregelatinized samples were shown to have the opposite result, such that the smallest particle size of pregelatinized sample had the lowest sinkability (11.3%). The turbidity of the pregelatinized small particle size was the highest by a factor of 1.08.
Anti-staphylococcal Bacteriocin from Enterococcus faecium
Kim, Kyung-Suk ; Lee, Ung-Soo ; Moon, Gi-Seong ;
Preventive Nutrition and Food Science, volume 15, issue 1, 2010, Pages 74~77
DOI : 10.3746/jfn.2010.15.1.074
Antibiotic-resistant Staphylococcus aureus is beginning to pose a social issue. Thus, there is an urgent need for the development of effective anti-staphylococcal agents to eradicate antibiotic-resistant S. aureus in food systems and to treat the pathogen in clinical areas. To address this need, lactic acid bacteria (LAB) from kimchi were screened for the production of anti-staphylococcal bacteriocin. From this screening, a bacteriocin generated by the MK3 strain, which was identified by 16S rRNA gene sequence analysis as Enterococcus faecium, demonstrated antimicrobial activity against an S. aureus strain, and was designated enterocin MK3. Enterocin MK3 also demonstrated activity against other gram-positive bacteria, including several LAB and Listeria monocytogenes, but not gram-negative Escherichia coli. The molecular mass of enterocin MK3 was estimated as approximately 6.5 kDa on an SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) gel.
Effects of Water-Soluble Calcium Supplements Made from Eggshells and Oyster Shells on the Calcium Metabolism of Growing Rats
Jang, Se-Young ; Jeong, Yong-Jin ; Kwon, Taeg-Kyu ; Seo, Ji-Hyung ;
Preventive Nutrition and Food Science, volume 15, issue 1, 2010, Pages 78~82
DOI : 10.3746/jfn.2010.15.1.078
This study investigated the effects of water-soluble calcium supplements manufactured with eggshells and oyster shells on growing rats. The aim was to review the potential use of food wastes as materials for water-soluble calcium supplements as compared to water-soluble calcium supplements made from imported seaweed powder. When experimental animals were administered three types of water-soluble calcium supplements orally for six weeks, the serum calcium level of the seaweed calcium supplement group were significantly higher than that of eggshell or oyster shell-derived calcium, but blood alkali phosphatase activity, osteocalcin and urine crosslink levels were not different in the three types of calcium supplements. Bone mineral density and bone mineral content in spine, femur and tibia also were not significantly different among the groups. However, when considering body weight of each group, bone mineral density and bone mineral content of the femur were significantly higher in the oyster shell calcium supplement group. These results suggest that at least on a short-term basis, the effect of calcium supplements prepared from eggshell and oyster shell are similar to the effects of seaweed calcium supplements.