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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Preventive Nutrition and Food Science
Journal Basic Information
Journal DOI :
The Korean Society of Food Science and Nutrition
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Volume & Issues
Volume 3, Issue 4 - Dec 1998
Volume 3, Issue 3 - Sep 1998
Volume 3, Issue 2 - Jun 1998
Volume 3, Issue 1 - Mar 1998
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Iron-Solubilizing Isolate of Meat : Physiological , Compositional and Physicochemical Characteristics
Kim, Yunji ; Charles E.Carpenter ; Arthur W.Mahoney ;
Preventive Nutrition and Food Science, volume 3, issue 1, 1998, Pages 1~9
Studies were performed to 1) investigate if ferric iron bound in complex with iron-solubilizing meat components is absorbable, 2) compare the relative iron-solubilizing capaicty of meats, and 3) investigate the physicochemical and compositional characteristics if meat meat has iron-solubilizing components . Iron-solubilizing components of beef were isolate from pH 2 HCL homogenates into dialysis bags(MWCO of 6-8K). Radiolabelled iron complexes were then generated using ferric iron and the isolated low-molecular-weight components(ILC) from undigested beef or ascrobate. The bioavailabilities of radioiron in these complexes or as ferric iron were measured as radioiron absorption into the blood one hour after injection into ligated duodenal lops of rats. Iron absorptions were ferrous -ascorbate complexes(18.8
2.2%)> ferric-ILC complexes(4.9
0.6%)>ferric iron (23.2
0.3%)(p<0.05). ILC from 0.1g of beef, pork, chicken, fish , or egg white were added to 400
g ferric iron in pH 2 HCL, the pH raised to 7,2, and soluble iron determined in the supernatant after centrifugation at 2,500g for 10 min. Iron solubilizing capabilities of ILC were pork (99.9
3.5%)> chicken (75.8
1.8%) > fish(64.6%
0.9%)(p<0.05). The compositional and physico-chemical characteristics of the ILC from the above dietary protein sources were investigated.
Development of Blue Musel Hydrolysate as a Flavouring
Cha, Yong-Jun ; Kim, Hun ; Kim, Eun-Jeong ;
Preventive Nutrition and Food Science, volume 3, issue 1, 1998, Pages 10~14
The hydrolysis conditions of blue mussel were evaluated by response surface methodology(RSM) for the alkaline protease Optimise TM APL-440 . Conditions favoring the highest degree of hydrolysis in blue mussel were pH 9,8, 58
reaction temperature, 2,9 hrs reaction time, 46.8%(w/v) substrate concentration, and 0.34%(v/w) enzym
strate ratio. Levels of n-3 fatty acids, e.g.C18 : 3, C18 : 4, and C20 : 5, did not change after hydrolysis in blue mussel sample.le.
Flavor and Taste-Active Compounds in Blue Mussel Hydrolysate Produced by Protease
Cha, Yong-Jun ; Kim, Hun ; Jang, Sung-Min ;
Preventive Nutrition and Food Science, volume 3, issue 1, 1998, Pages 15~21
Volatile flavor compounds in hydrolyzed blue mussel(HBM) produced by OptimaseTM APL-440, with untreated blue mussel(UBM) were compared. A total of 100 volatile compounds were detected in both HBM and YBM , consisting mainly of 25 aldehydes, 16 ketones, 17 alcohols, 8 nitrogen-containing compounds, 11 aromatic compounds, 8 terpenes, and 15 miscellaneous compounds. Levels of aromiatic compounds decreased after hydrolysis, whereas levels of 7 nitrogen-containing compounds increased. The compounds , 3-methylbutanal, (z)-4-heptenal, and (E,Z)-2-, 6-nonadienal , had the highest odor values in both samples. Total free amino acids in HBM were 21.89%(w/w) and increased by 3,4 times higher than UBM. glutamic acid and aspartic acid, having sour tastes, were the major taste-active compounds in HBM.
Detection of Irradiated Model Food Containing Salt by Thermoluminescence Measurement
Chung, Hyung-Wook ; Kwon, Joong-Ho ;
Preventive Nutrition and Food Science, volume 3, issue 1, 1998, Pages 22~26
Model food containing common salt(NaCl) was subjected to the thermoluminescene(TL) detection whether it is irradiated or not. Salt irradiated with
-gamma ray and electron beam exhibited a characteristic TL gowcurve depending on the irradiation dose, showing major peaks at
. The intensity of TL glowcurves was directly proportional to the irradiated doses regardless of irradiation sources at each concentration of salt. A high correlation coefficient was observed for irradiated salt between the irradiation doses and the corresponding TL responses. At the same dose, the intensity of TL glowcurve increased as the concentration of salt increased in the test sample. TL glowcurves of nonirradiated salt and irradiated model food without salt were negligible and similar to a baseline . However, irradiated model food containing salt gave rise to a characteristic TL glowcurve with two major peaks at about
, respectively. The results showed that salt played a role as an internla as well as external indicator in TL measurements, indicating that TL will be applicable to other condiments and spices with salt for their detection whether they are irradiated or not.
Thermal-and Bio-degradation of Starch-Polyethylene Films Containing High Molecular Weight Oxidized-Polyethylene
Kim, Mee-Ra ; Pometto, Anthony-L. ;
Preventive Nutrition and Food Science, volume 3, issue 1, 1998, Pages 27~35
Starch-polyethylene films containing high molecular weight(NW) oxidized-polyethylene and prooxidant were prepared , and thermal -and bio-degradability of the films were determined. Increased levels of starch resulted in a corresponding reduction in mechanical strength of the films. However, the addition of high MW oxidized-polyethylene did not significantly reduce the percent elongation of the films. Thefilms containing high MW oxidized-polyethylene andproosicant were degreaded faster than those containing no aadditive during the heat treatment. The films lost their measureable mechanical properties when their weight-average MW(Mw) fell below 50,000. Biodegradability of the films was determined by a pure culture assay with either Streptomyces badius 252.S. setonii 75Vi2 or S. viridosporous T7A, and by an extracellulr enzyme assay using S. setonii 75vi2. The results from pure culture assay indicated that biomass accumulation on the film surface inhibited chemical and biological degradation of the films. The extracellular enzyme assay demonstrated decrease of percent elongation and increase of carbonyl index of the films. Therefore, extracellular enzyme assay could be used as a good method to evaluate biodegradability of the films.
Potentimetric Biosensor for Detection of L-Malate and D-Isocitrate Employing
-Selective Electrode and Enzyme Immobilization in Flow Injection Analysis
Kwun, In-Sook ; Kim, Meera ;
Preventive Nutrition and Food Science, volume 3, issue 1, 1998, Pages 36~42
Ion-selective eleltrodes(ISEs) are simple electrodechemical devices for the direct measurement of ions in the samples. A novel potentiometric biosensor for the determination of L-Malate or D-isocitrate has been developed by using CO2-3 -ISE-FIA system was composed of a pump, an injector, a malic enzyme or isocitric dehydrogenase enzyme reactor, a CO2-3 -ISE, a pH/mV meter, and an integrater. The various factors, such as buffer capacity types of plstericizer and polymer, were optimized for the CO2-3 selectivity. In this novel CO2-3 --ISE-FIA system, the potential difference due to the amount of CO2-3 produced from each enzyme reaction was proportional to the amount of L-malate or D-isocitrate.
Incidence of Fusarium and other Molds in Korean Field Crops
Ryu, Dojin ; Bullerman, Lloyd B. ;
Preventive Nutrition and Food Science, volume 3, issue 1, 1998, Pages 43~47
The incidence of total molds, Fusarium species, and the estrogenic mycotoxin,zearalenone, in Korean grain samples were investigated . The majority of molds infecting grain were identified as belonging to the genus Alternaria , with an average infection rate of kernels of 43% and 32% in rice and baley, respectively. Fusarium speciens were less common, with average infection rates of 13% and 19% in reice and barley, respectively. A number of field fungi including Curvularia and Dactylaria were also observed. Among the Fusarium speices, 71 of 94 Fusarium isolates were identified as F.semitectum. A few F. moniliforme and F. equiseti were observed linked immunosorbent assay (ELISA) or high-performance liquid chromatography(HPLC). In addition, deoxynivalenol was not deteted by ELISA . However, thepresence of molds, including Fusarium species, may pose possbile health hazards to persons consuming those grains.
Effect of Allopurinol on the Ethanol-induced Oxidative Stress : Mechanism of Allopurinol Action
Park, Min-Kyung ;
Preventive Nutrition and Food Science, volume 3, issue 1, 1998, Pages 48~55
An acute ethanol load(50mmol/kg , i.p) resulted in an increase in peroxidation and a decrease in the levels of
-tocopherol and ascorbate in rat cerebellum. Pretreatement with allopurinol(146
mol/kg, i.p) prevented the ethnol-induced increment in lipid peroxidation and decrease in
-tocopherol content. However, the decrease of ascorbate was of greater magnitude when allopurinol was associated with ethanol. These results suggested that allopurinol. besides its action as a radical scavenger and xanthine oxidase inhibitor, might favor the regeneration of
-tocopherol antioxidant acitviity was studied using
-radiolysis in aerated ethanolic solutions. Even though allopurinol did not react by itself with
-hydroxyethyl-peroxyl radicals [H3C-CH(OH)OO] , it enhance the
-hydroxyethyl-peroxyl radical scavenging properties of
tocopherol. The regeneration of
-tocopherol from the
-hydroxyethyl-peroxyl radical scavenging properties of
-tocophero. The regeneration of
-tocopherol from the
-tocopherol radical by ascorbate remained as efficient in the presence of allopurinol as in its absence. The effects of allopurinol on the Vitamin E oxidation-reduction mechanism could be involoved in the beneficial effectof allopurinol on the biological cellular damages linked to free radical reactions.
Effect of Dietary Vitamin A on Plasma Membrane Property and Ultrastructure in Ethanol -administered Rat Liver
Yang, Kyung-Mi ; Seo, Jung-Sook ; Cho, Hyun-Gug ;
Preventive Nutrition and Food Science, volume 3, issue 1, 1998, Pages 56~61
This study was conducted to investigate the effect of dietary supplementation of vitamin A on the membrane property and ultrastructure in ethanol-administered rat livers. Male Sprague-Dawley rats weighing of 130 ~150g were fed with experimental diets for 7 weeks. The diets contained different types of vitamin A which were
-carotene, retinyl acetate and retinoic acid. After feeding theexperimental diets for 7 weeks, a dose of 3.0g ethanol (30%, W/V)/kg B.W was injected to rats intraperitoneally. Control rats received 0.9% saline containing isocaloric sucrose instead of ethanol. Plasma membrane fluidity of liver decreased in rats fed with vitamin a -Deficient diet with ethanol as compared to that of control rats. Fluidity change of liver plasma membrane that ethanol had induced was influenced by dietary supplementation of vitamin A, but not influenced by the type of supplemented vitamin. A . The ultrastructural changed of hepatic mitrochondria were observed in some rats such as vitamin A-deficient rats with ethanol. Inadequate consumptionof vitamin A contributed to ultrastructural changes such as swelled mitochondria occurred by ethanol-induced hepatotoxicity. Although accurate mechanism involved in the plasma membrane-stabilizing effect of vitamin A is still unclear, dietary supplementation of vitamin A such as retinyl acetate is neede to modulate this change. The direct involvement of membrane property on the cell damage caused by ethanol treatment remains to be established.
Effect of Dietary Capsaicin on Hepatic Drug-Metabolizing Enzyme Activities in Mice
Kim, Jung-Mi ; Kim, Dong-Hyun ; Choe, Suck-Young ; Rina Yu ;
Preventive Nutrition and Food Science, volume 3, issue 1, 1998, Pages 62~66
The effect of dietary capsaicin (8-methyl-N-vanillyl-6-nonenamide, CAP) on drug-metabolizing enzyme activities was investigated in mice. Male ICR mice were divided into 4 groups and fed diets containing 0, 5, 20, 100 ppm CAP for 4 seeks. Hepatic drug-metabolizing enzyme activities and serum alanine aminotransferase and aspartate transaminease activities were measured. There was no difference in hepatic alanine aminotransferse and aspartate transaminase activities among the groups. Hepatic microsomal cytochrome P450 in CAP fed groups, but p-nitrophenol hydroxylase and the cytosolic acitivity of glutathione S-transferase activities were decreased in the dietary CAP supplemetned groups compared to the control. These results suggest that the dietary CAP at a low dose differentially modulates drug-metabolizing enzyme acitvities without causing hepatic toxicity.
Effect of Gam-Tea on the Metabolizing Enzyme Activity of Some Free Radical and Alcohol in Rats
Yoon, Chong-Guk ; Chae, Soon-Nim ; Shin, Joong-Kyu ;
Preventive Nutrition and Food Science, volume 3, issue 1, 1998, Pages 67~70
To investigate an effect of Gam-Roa tea on the free radical or alcohol detoxicating enzyme activities, the rats got a drink at the Gam-Roa tea instead of water for 3 months, and then the animals were sacrificed and obtained the following findings. The animals receiving Gam-Roa tea showed a decreasing tendency of hepatic xanthine oxithine oxidase activity and significantly incresed content of cytochrome P-450 compared with the control. Furthermore, hepatic superoxide dismutase and glutathione S-transferase activities were also more increased in rats received Gam-Roa tea than in the control group, those receiving water. On the other hand, alcohol or aldehyde dehydrogenase activities were more increased in rats receiving Gam-Roa tea than the control. In conclusion, it is likely that the liver of rats receiving Gam-Roa tea may have the oxygen free radical or alcohol detoxication potential.
Regular Exercise-training Affects Serum Lipid and Carnitine Profiles in Some College Students
Cha, Youn-Soo ; Jung, Bok-Mi ; Kim, Hyeung-Rak ; Ahn, Chang-Bum ; Lim, Sang-Sun ;
Preventive Nutrition and Food Science, volume 3, issue 1, 1998, Pages 71~76
This study investigated the effect of regular exercise on serum lipid profiles and carnitine levels in college students. Daily nutrient intake, anthropometry , serum lipid, and carnitine profiles in serum and urine were evaluated prior to beginning the study and after 35 days of treadmill running for 30 minutes per day. The results obtained were summarized as follows : 1) Concentrations of total lipid and Triglyceride in serum were decreased by regular exercise in female subjects but unaffected in males. 2) Serum LDL-cholesterol was significantly decreased, but total cholesterol and HDL-cholesterol in serum were not affected in both male and female subjects. 3) nonesterified carnitinem, acid-insoluble acylcarnitine, and total carnitine levels in serum were not affected, but acid-soluble acylcarnitine level was increased by regular exercise in both subjects. 4) Urinary excretionof the acid-soluble acylcarnitine level was increased by regular exercise -training. These results suggest that regular exercise -training has different effects on serum lipid oxidation via carnitine metabolism in this condition.
Food Consumption Patterns of First Generation Korean-Americans in Hawaii
Han, ji-Sook ;
Preventive Nutrition and Food Science, volume 3, issue 1, 1998, Pages 77~84
To evaluate food consumption patterns of first generation Korean-American in Hawaii, questionnaires were developed using sociodemographic questions and food frequency questionnaire, which included 139 food items most often consumed among Korean foods and American foods. The questionnaires surveyed 157 first generation Korean-Americans in Hawaii. Mean daily servings for the first generation Korean-Americans were calculated for 139 food items combined into 41 food groups based on similarity in nutrient composition and serving size. The food groups which were consumed in amounts over one serving per day for all subjects were rice, Kimchi , non-citrus fruit , vegetables, organge/green vegetables. oil. margarine and coffee/tea. All subjects consumed less than one serving of hotdogs, hamburgers, pizza and pancakes per week(0.14 serving per day). The most notable characteristic of food consumption for first generation Korean-Americans was that they consumed more Korean food such as rice, Kimchi, soybean paste(Deenjang), soybean curd and seaweed than American foods. Compared with other groups based on age and gender, younger men showed significantly(p<0.05) more frequent consumption of beef/pork, sausages /hams /bacons and hambergers. Older men were significantly(p<0.05) more likely to consume Doenjang and less likely to consume pizza and hamburgers. Daily servings were below the recommended level for thegrains /bread/cereals group and fats/oils/sweets group for all subjects . Fruits/vegetables group servings exceeded the recommeded 5 daily servings for younger men. In correlations of daily servings of selected foods among Korean foods and American food with sociodemographic characteristics, this study showed that the older the subjects and the shorter the stay in Hawaii, subjects were more likely to consume Kroean foods.
Effects of Vitamins E and C on Human BreastCancer Cell Growth in the Presence of Various Fatty Acids
Kim, Gun-Hee ; Cho, Il-Jin ; Oh, Sun-Hee ; Park, Hee-Sung ; Cho, Sung-Hee ;
Preventive Nutrition and Food Science, volume 3, issue 1, 1998, Pages 85~91
To investigate the effects of antioxidative vitamins in combination with various fatty acids on breast cancer cell proliferation, MDA-MB231 human breast cancer cells were cultured for 3 days in the serum-free Iscove's modified Dulbecco's medium (IMDM) supplemented with 1.25mg/ml delipidized bovine serum albumin and 10㎍/ml insulin. Alpha-tocopherol, ascorbic acid or both vitamins were added to the medium at the concentrations of 10 and 50μM in the presence of 3μg/ml of oletic(Oa), linoleic(LA) α-linoleinic(LNA) and docosahexaenoic acid(DHA). Cell growth was reduced significantly by α-tocopherol in a dose-dependent manner, but not affected by ascorbic aicd. The four different fatty acids did not have significant effects on cell growth, although DHA exerted inhibitory effect on the growth after 1 day. However, the each fatty acid was well incorporated into celluar lipid as such or elongated forms. Addition of α-tocopherol remarkably increased its celluar contents and reduced cellular levels of thiobarbituric acid substances (TBARS) that were elevated notably in the presence of DHA in the culture media. But ascorbic acid addition did not change much of either cellular α-tocopherol or TBARS contents. northern blot hybridization showed that tumor supressor gene ρ53 was most highly expressed by the combination of ρ-tocopherol and DHA in 8 hours of cell culture. In conclusion , the growth inhibitory effect of vitamin E suggests that breast cancer cell proliferation is reduced by the mechanism other than cytotoxicity of lipid peroxide and it is related to expressionof tumor supprosser gene p53, that can be increased by both vitamin E and n-3 fatty acid, DHA.
Antitumorigenic Effects of Tannin From Persimmon Leaves on Sarcoma 180-induced Tumor in Mice
Moon, Sung-Chai ; Park, Kyong-Hee ; Rhew, Tae-Hyong ; Park, Kun-Young ; Kim, Byeong-Gee ;
Preventive Nutrition and Food Science, volume 3, issue 1, 1998, Pages 92~97
The changes of morphology and protein pattern of sarcoma 180 cells treated with or without trannins extracted from persimmon leaves were evaluated by light microscopy, electrophoresis and Western blotting. The sarcoma 180 cells treated with tannins increased the amount of proteins which presumably were intermediate filament cytokeratins detected by electrophoresis and Western blot. Tannins was indirectly cytotoxic to the sarcoma 180 cells and increased the intermediate filament protein level in the cells.
Expression of Starch-degrading Genes in Escherichia Coli and Kactococcus Lactis
Jeong, Jong-Jin ; Kim, Tea-Youn ; Moon, Gi-Seong ; Lee, Hyo-Jeong ; Kim, Jong-Sang ; Kim, Jeong -Hwan ;
Preventive Nutrition and Food Science, volume 3, issue 1, 1998, Pages 98~104
As an efffort ot construct LAB (latice acid bacteria), capable of utilizing starch as fermentation substrate without the aid of externally supplied enzymes, plasmid vectors containing the amyL(
-amylase/pullulansase gene) from Clostridium thermophydrosulfuricum, and glucoamylase cDNA from Asperigillus shirousamii were constructed and introduced itno E. coli and L. lactis. For expression in procaryotes , 1.9kb glucoamylase cDNA encoding the mature form of enzyme was PCR amplified and translationaly fused to a PCR amplified 260 bp fragment containing the promotor and secretion signals of amyl in the same reading frame. The production of
-amylase, Apu, and glucoamlase in E. coli and L. lactis was confirmed by enzyme assay and zymography . Enzymeswere detected in both cellpellets and supernatants, indicating theworking of scretion signals in heterologous hosts. The efficiencies of secretion were varibale depending on the gene and host. The highest
- amylase acitivity observed was 1.1 units and most activiity was detected from thecell pellets. The degree of gene expression in both hosts and the effect on the growth of hosts were examined.