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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Preventive Nutrition and Food Science
Journal Basic Information
Journal DOI :
The Korean Society of Food Science and Nutrition
Editor in Chief :
Volume & Issues
Volume 5, Issue 4 - Dec 2000
Volume 5, Issue 3 - Sep 2000
Volume 5, Issue 2 - Jun 2000
Volume 5, Issue 1 - Mar 2000
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Rapid Formation of biologically Active Neoglycoprotein from Lysozyme and Xyloglucan Hydrolysates through Naturally Occurring Maillard Reaction
Soichiro Nakamura ; Masayoshi Saito ; Tetsuhisa Goto ; Hiroki Seaki ; Masahiro Ogawa ; Masayuki Gotoh ; Yasuhide Gohya ; Hwang, Jae-Kwan ;
Preventive Nutrition and Food Science, volume 5, issue 2, 2000, Pages 65~69
Hen egg-white lysozyme was conjugated with 7~9 mers xyloglucan hydrolysates(MW-1,400) at 6
and 79% relative humidity for 3 days. SDS-PAGE showed that the conjugation between lysozyme and the oligosaccharide began from 1-day incubation, and three molecules of carbohydrate chains were attached to a protein molecule after 30day incubation. The enzymatic activity of lysozyme was totally conserved in the neoglycoprotein, when measured by using glycol chitin as substrate. Besides, the emulsifying properties of lysozyme were vastly improved by the conjugation with the oligosaccharide, in which emulsifying activity of the neoglycoprotein was five times higher than that of native one.
Synthesis of Triglyceride of Conjugated Linoleic Acid(CLA) by Lipozyme
Park, Won-Seck ; Kim, Seck-Jong ; Park, Kyung-Ah ; Kim, Jeong-Ok ; Lee, Eun-Joo ; Lim, Dong-Gil ; Ha, Yeong-Lae ;
Preventive Nutrition and Food Science, volume 5, issue 2, 2000, Pages 70~74
Most fatty acids in food matrices are triglyceride(TG) forms. Conjugated linoleic acid(CLA) produced from linoleic acid by microorganisms or chemicals is a free form. To apply the CLA to food systems, the TG containing CLA(designate CLA-TG) was synthesized by Lipozyme-catalyzed esterification method. An optimum reaction con-dition for the esterification of free CLA(FCLA) to glycerol by Lipozyme was determined as follows; Lipozyme(50mg) effectively catalyzed the esterification of CLA(500mg) to glycerol(1150mg) dissolved in isooctane (3ml) in a shaking incubator (200 rpm, 5
) for 48 hr. Under the reaction condition, the resultant contained 52.4% CLA-TG as well as 31.1% Di-CLA-glycerol(CLA-DG), 7.6% mono-CLA-glycerol(CLA-MG), and 9.0% other CLA(un-reacted FCLA plus CLA dimer). These results suggest that the Lipozyme could be a useful enzyme for the production of CLA-TG to be employed in foods.
Purification and Characterization of Angiotensin I-Converting Enzyme Inhibitors from Sinapis alba L.
Yuk, Jin-Su ; Lim, Young-Hee ; Cho, Hong-Yon ;
Preventive Nutrition and Food Science, volume 5, issue 2, 2000, Pages 75~80
To separate ACE inhibitors from edible plants, spices, and herbs, 285 extracts of 95 sources were screened for ACE inhibitory activity. The extract of Sinapis alba L. had the most potent ACE inhibitory activity. Mustard seeds were crushed homogeneously and extracted with hexane and water successively. Lyophilized water extract was fractionated with
:butanol(1:1). The ACE inhibitor was purified from butanol fraction by methanol precipi-tation, gel filtration, HPLC, and FPLC with Superdex peptide HQ 10/30 column. The active fraction has been purified to homogeneity, which was proven by gel filtration using FPLC system. The yield was 0.02%. The com-pound has a molecular weight of about 640. The compound competitively inhibited ACE activity and the
value was 79
/ml. The purified compound showed uterus contraction activity in isolated rat uterus.
Encapsulation of Bromelain in Liposome
Lee, Dong-Hoon ; Jin, Bong-Hwa ; Hwang, Yong-Il ; Lee, Seung-Cheol ;
Preventive Nutrition and Food Science, volume 5, issue 2, 2000, Pages 81~85
Bromelain has been used as a meat-tenderizing agent in food processing. To increase the availability of bromelain, microencapsulation into liposome was carried out by the dehydration and rehydration method. Small unilamellar vesicles prepared by sonication treatment showed higher encapsulation efficiency (EE) than by the French press method. In the preparation of liposome, the effect of pH and centrifugal force on EE was also investigated and it showed a higher EE at acidic pH than at alkaline pH with centrifugation at 80, 000
g. The actual EEs except unencapsulated bromelain which bound on the outside surface of liposome by electrostatic interaction also were investigated, and the optimal EE was at pH 4.6, at 0.6 of a ratio of bromelain to phosholipid(18.2%, w/w). Release of bromelain from liposomes was stimulated as the temperature increased at neutral pH.
Preparation of a Large Quantity of CIS-9, trans-11 and trans-10, cis-12 Conjugated Linoleic Acid(CLA) Isomers from SYnthetic CLA
Kim, Seck-Jong ; Park, Kyung-Ah ; Park, Jung-H.Y. ; Kim, Jeong-Ok ; Ha, Yeong-Lae ;
Preventive Nutrition and Food Science, volume 5, issue 2, 2000, Pages 86~92
Conjugated linoleic acid(CLA) refers to a collective term of positional and geometric isomers of linoleic acid, which are different in their biological activities. The predominant isomer of CLA in animal tissues is cis-9, trans-11; smaller amounts of trans-10, cis-12 CLA isomers, CLA methyl ester (CLA-ME) was chemically syn-thesized from linoleic acid by the alkaline isomerization method. The synthetic CLA-ME, mainly composed of cis-9, trans-11 CLA and trans-10, cis-12 CLA, was dissolved in acetone, stored at 68
for 1 day, and the supernatant(cis-9, trans-11 CLA-Me) was separated from the precipitate (trans-10, cis-12 CLA-Me). After the processes were repeated three times at -68
, the whole processes were repeated three times at -71
in order to increase the purity of these two isomers. The cis-9, trans-11 CLA-Me and trans-10, cis-12 CLA isomers were further purified by the urea adduct. Purities of the cis-9, trans-11 CLA-Me and trans-10, cis-12 CLA-Me were 90.3 and 99.9%, respec-tively. This method could be employed for the preparation of a large quantity of highly purified cis-9, trans-11 CLA-Me or trans-10, cis-12 CLA-Me from synthetic CLA-Me.
Detection of Irradiated Cereals by Viscosity Measurement
Yi, Sang-Duk ; Chang, Kyu-Seob ; Yang, Jae-Seung ;
Preventive Nutrition and Food Science, volume 5, issue 2, 2000, Pages 93~99
A study was performed to establish the detection method of irradiated cereals. A drastic reduction of the apparent viscosity of suspensions with heat treatment was observed up to 1∼2 kGy in brown rice, Job's-tears, polished barley and polished rice. They were gently reduced to samples irradiated at 15 kGy. This trend was similar for all stirring speeds. The viscosity of unirradiated brown rice, Job's-tears, polished barley and polished rice reduced with in-creasing stirring speeds and this tendency was similar for irradiation doses. Regression expressions and coefficients of brown rice, Job's s-tears, polished barley and polished rice on different doses were 0.9399(
) and 0.9714(
), respectively, at 120 rpm. These results sug-gest that detection of irradiation for cereals could be possible by viscometric methods.
Energy Value of Carbohydrate and Lipids with Added Calcium for Growing Mice
Khalil, Dania A. ; Owens, Fredric N. ; Hanson, Christa F. ;
Preventive Nutrition and Food Science, volume 5, issue 2, 2000, Pages 100~104
The caloric contribution of diets supplemented with sucrose, corn oil, or tallow with or without additional calcium was examined using female CD1 weanling mice. Mice were limit-fed a semi-purified diet alone or with added isocaloric amounts from sucrose, corn oil, or tallow for 28 days. In addition, diets with suppelmental fat contained either 0.60% or 1.5% calcium. Fecal fat and fecal soap excretions were greater (p<0.06) for mice fed tallow than for those fed corn oil. Mean metabolizable energy values for sucrose, tallow, and corn oil averaged 4.01, 7.96, and 8.94 kcal, respectively. Retention of digested energy from sucrose, tallow and corn oil averaged 13%, 10% and 21%, respectively. Hence, per gram of added nutrient, retained energy from tallow averaged 1.60 and that from corn oil averaged 4.11 times that of added sucrose. Retained energy from added corn oil was greater (p<0.01) than from added tallow. On a retained energy basis, the relative value for corn oil was greater and the relative value for tallow was less than the metabolizable energy ratio of fat to carbohydrate proposed by Atwater of 2.25.Added calcium depressed(p<0.01) digestibilities of both dry matter and energy with a greater(p<0.01) effect on tallow than on corn oil. These findings imply that the source of fat and calcium in the diet influence the avail-ability energy in diets and should be considered in feed formulations.
Effect of Tungstate Supplemented Diet on the Toluene Metabolism in Rats
Chae, Soon-Nim ; Jeon, Tae-Won ; Yoon, Chong-Guk ;
Preventive Nutrition and Food Science, volume 5, issue 2, 2000, Pages 105~108
To evaluate an effect of oxygen free radical on the toluence metabolism, the rats were fed on a tungstate sup-plemented diet(0.75g of tungstate included in 1kg of standard diet) or a standard diet. To the present xanthine oxidase deficient animal model, toluene(0.15ml/100g of body weight) was injected and then the animals were sacrificed after 24 hrs to determine the toluene metabolizing enzyme activities and toluene metabolite, hippuric acid concentration. The increasing rate of urinary hippuric acid concentration was significantly(p<0.01) higher in tungstate fed animals than in standard diet fed ones. Hepatic cytochrome P_450 contents were significantly higher(p<0.01) in tungstate fed animals than in standard diet fed ones. And tungstate fed animals showed a ten-dency of higher activities of benzylalcohol dehydrogenase while a significantly higher activites of benzaldehyde dehydrogenase (p<0.01) than standard diet fed animals. In conclusion, the more possibly reduced oxygen free radical in toluene-treated rats fed with a tungstate supplemented diet than in those fed with a standard diet would be responsible for the enhancement of toluene metabolism.
Hesperidin Lowers Activities of 3-Hydroxy-3-methylglutaryl-CoA Reductase and Acyl-CoA:Cholesterol Acyltransferase in Rats Fed High -Cholesterol Diet
Park, Yong-Bok ; Jeong, Tae-Sook ; Lee, Sung-Heui ; Bok, Song-Hae ; Kwon, Yong-Kook ; Bae, Ki-Hwan ; Lee, Mi-Kyung ; Choi, Myung-Sook ;
Preventive Nutrition and Food Science, volume 5, issue 2, 2000, Pages 109~113
In this study, a potential mechanism through which the hesperidin might work on the effect was examined in vivo. Male rats were fed a high cholesterol synthetic diet (1%, wt/wt) with hesperidin (0.1%, wt/wt) for 42 days. Activity of hepatic HMG-CoA reductase was significantly lowered by the hesperidin supplement compared to the control. Hesperidin did not significantly alter plasma or hepatic lipids, but tended to lower those lipid levels. Hesperidin also subsequently reduced the fecal neutral sterols compared to the control(253.3mg/d vs.521.9 mg/d). The inhibition of HMG-CoA reductase resulting from the hesperidin supplementation could count for the reduction in fecal neutral sterols that appears to compensate for the decreased cholesterol biosynthesis. The dose of hesperidin in a high choles-terol diet should apparently be more than 0.1% to exhibit the hypocholesterolemic response in these rats. It remains to be determined whether the observed alterations in cholesterol metabolism are specific to the rat or also could be applied to the humans.
Inhibitory Effect of Doenjang(fermented Korean soy paste) Extracts and Linoleic Acid on the Growth of Human Cancer Cell Lines
Park, Kun-Young ; Lee, Jeong-Min ; Moon, Suk-Hee ; Jung, Keun-Ok ;
Preventive Nutrition and Food Science, volume 5, issue 2, 2000, Pages 114~118
The inhibitory effects of doenjang extracts and linoleic acid(LA) which was identified as one of the active compounds in doenjang on the growth of human cancer cells were studied, comparing to the actions on normal cells. Methanol extract and hexane fraction from doenjang exhibited the strong growth inhibitory effect on HT-29 human colon carcinoma cells. Inhibitory effects of chloroform, ethyl acetate, butanol and aqueous fractions on the cancer cells were observed, moderately or weakly. When cell counts of SNU-C
human colon carcinoma cells were determined daily for 6 days, the inhibitory effect of hexane fraction on this cell line was higher than that of the methanol extract from doenjang. LA completely suppressed the growth of SNU-C
cells after 4 days, while conjugated linoleic acid(CLA) resulted in 98% inhibition after 6 days. With the addition of LA and other free fatty acids such as stearic acid, oleic acid, linolenic acid and
-linolenic acid (
-LnA) to the culture system, the growth of HT-29 cells and SNU-C
cells was greatly suppressed after 6 days. Inhibitory effects of LA
-LnA on the growth of these cells were stronger than other fatty acids. On the growth of AZ-521 human gastric carcinoma cells, LA and CLA completely cuppressed the growth of the cells after 4 days and 3 days, respectively. At the level of 0.001%~0.01% of LA, there was no cytotoxic effect on normal rat kidney cells and normal intestine human cells. These results showed that LA, a major active compound of doenjang, had strong inhibitory effects on the growth of human cancer cells without damaging normal cells.