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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Fisheries and aquatic sciences
Journal Basic Information
Journal DOI :
The Korean Society of Fisheries and Aquatic Science
Editor in Chief :
Sungchul C. Bai
Volume & Issues
Volume 14, Issue 4 - Dec 2011
Volume 14, Issue 3 - Sep 2011
Volume 14, Issue 2 - Jun 2011
Volume 14, Issue 1 - Mar 2011
Selecting the target year
The Effects of Alkaline Treatment and Potato-Starch Content on the Quality of Fish Meat Paste Products Prepared from Pacific Sandlance Ammodytes personatus Girard
Yoo, Byung-Jin ;
Fisheries and aquatic sciences, volume 14, issue 3, 2011, Pages 161~167
DOI : 10.5657/FAS.2011.0161
This study investigated the effects of the number of washes and alkaline treatments (
concentrations) and the concentration of potato starch on the quality of fish meat paste products prepared from sand lance Ammodytes personatus Girard. We found significantly (P<0.05) higher ratings for the all textural parameters (hardness, brittleness, elasticity, and cohesiveness) of the sand-lance meat paste products (SLMPPs) that were washed three times with a 0.5%
concentration. We determined that an 8% concentration of potato starch leads to the best textural properties in SLMPPs. As the amount of potato starch was increased, the redness values of SLMPPs decreased significantly (P<0.05), but the differences in the sensory evaluation parameters (texture, flavor, taste, and overall acceptability) between the SLMPPs were not significant.
Fractionation and Characterization of Fractions with High Antioxidative Activity from the Gelatin Hydrolysates of Korean Rockfish Sebastes schlegelii Skin
Kim, Hyung-Jun ; Park, Kwon-Hyun ; Shin, Jun-Ho ; Lee, Ji-Sun ; Heu, Min-Soo ; Lee, Dong-Ho ; Kim, Jin-Soo ;
Fisheries and aquatic sciences, volume 14, issue 3, 2011, Pages 168~173
DOI : 10.5657/FAS.2011.0168
The purpose of this study was to obtain a fraction with high antioxidative activity from second rockfish gelatin hydrolysates (SRSGHs), which were hydrolyzed with Alcalase and Flavourzyme through ultrafiltration membranes with serial digestions for 1 and 2 h, respectively, and to investigate the feasibility of this fraction as a potential functional food ingredient. Among various fractions that were ultrafiltered from the SRSGH with four types of membrane (1, 5, 10, and 30 kDa), the SRSGH-III fraction, which permeated the 10 kDa membrane but not the 5 kDa membrane, showed the highest antioxidant activity (protection factor=5.13) and angiotensin-I-converting enzyme-inhibiting activity (
=0.82 mg/mL). These results suggest that the SRSGH-III fraction from the SRSGH can be used as a functional food ingredient. However, further studies examining its antioxidant activity in vivo as well as the different antioxidant mechanisms are needed.
ACE-Inhibitory Properties of Proteolytic Hydrolysates from Giant Jellyfish Nemopilema nomurai
Yoon, Ho-Dong ; Kim, Yeon-Kye ; Lim, Chi-Won ; Yeun, So-Mi ; Lee, Moon-Hee ; Moon, Ho-Sung ; Yoon, Na-Young ; Park, Hee-Yeon ; Lee, Doo-Seog ;
Fisheries and aquatic sciences, volume 14, issue 3, 2011, Pages 174~178
DOI : 10.5657/FAS.2011.0174
This study aimed to determine the degree of hydrolysis and angiotensin-I-converting enzyme (ACE)-inhibitory activity of Giant Jellyfish Nemopilema nomurai (jellyfish) hydrolysates. The degree of hydrolysis using six proteolytic enzymes (Alcalase, Flavozyme, Neutrase, papain, Protamex, and trypsin) ranged from 13.1-36.8% and the inhibitory activities from 20.46-79.58%. Using papain hydrolysate, we newly isolated and characterized ACE-inhibitory peptides with a molecular weight of 3,000-5,000 Da that originated from jellyfish collagen. The purified peptide (FII-b) was predicted to be produced from an alpha-2 fragment of the type IV collagen of jellyfish. The N-terminal sequence of FII-b was Asp-Pro-Gly-Leu-Glu-Gly-Ala-His-Gly- and showed 87% identity to the collagen type IV alpha-2 fragment of Rattus norvegicus and a predicted protein from Nematostella vectensis, indicating that the ACE-inhibitory peptide originated from the collagen hydrolysate and had an
value of 3.8
/mL. The primary structure of the fragment is now being studied; this peptide represents an interesting new type of ACE inhibitor and will provide knowledge of the potential applications of jellyfish components as therapies for hypertension.
In Vitro and Intracellular Antioxidant Activities of Brown Alga Eisenia bicyclis
Yoon, Na-Young ; Lee, Sang-Hoon ; Wijesekara, Isuru ; Kim, Se-Kwon ;
Fisheries and aquatic sciences, volume 14, issue 3, 2011, Pages 179~185
DOI : 10.5657/FAS.2011.0179
The antioxidant activities of a methanolic extract of Eisenia bicyclis and its organic solvent fractions, including dichloromethane (
), ethyl acetate (EtOAc), n-butanol (n-BuOH), and water (
) fractions, were investigated. Scavenging activities against DPPH, hydroxyl, superoxide anion, and peroxynitrite radicals were evaluated using electron spin resonance spectrometry; intracellular reactive oxygen species (ROS) were evaluated by a 2',7'-dichlorofluorescein diacetate assay using RAW264.7 mouse macrophages. The antioxidant activities of the individual fractions were: EtOAc>n-BuOH>
. The EtOAc fraction exhibited strong radical scavenging activity and a significantly reduced ROS level in RAW264.7 cells. Moreover, the phenolic contents of the extract and fractions followed the same order as their radical scavenging activities. Our results indicate that E. bicyclis is a valuable natural source of antioxidants that may be applicable to the functional food industry.
Isolation and Culture Properties of a Thermophilic Agarase-Producing Strain, Microbulbifer sp. SD-1
Kim, Do-Kyun ; Jang, Yu-Ri ; Kim, Kyoung-Hoon ; Lee, Mi-Nan ; Kim, A-Ra ; Jo, Eun-Ji ; Byun, Tae-Hwan ; Jeong, Eun-Tak ; Kwon, Hyun-Ju ; Kim, Byung-Woo ; Lee, Eun-Woo ;
Fisheries and aquatic sciences, volume 14, issue 3, 2011, Pages 186~191
DOI : 10.5657/FAS.2011.0186
An agar-degrading enzyme-producing strain was isolated from seawater. The isolate was identified as Microbulbifer sp. SD-1 by 16S rRNA sequencing analysis. The optimal pH and temperature for growth were 6.0 and
, respectively, and growth was possible at pH 9.0 and
. The isolate required 5% NaCl for optimal growth and showed 45% growth activity without NaCl. Agar concentrations of 0-0.4% in the medium did not affect growth. Thin-layer chromatography analysis revealed that this strain could degrade agar into a monosaccharide and oligosaccharide, which may have industrial applications.
Enhancement of Polyphenol Content and Antioxidant Activity of Brown Alga Eisenia bicyclis Extract by Microbial Fermentation
Eom, Sung-Hwan ; Kang, Young-Mi ; Park, Jae-Hong ; Yu, Dae-Ung ; Jeong, Eun-Tak ; Lee, Myung-Suk ; Kim, Young-Mog ;
Fisheries and aquatic sciences, volume 14, issue 3, 2011, Pages 192~197
DOI : 10.5657/FAS.2011.0192
The objective of this study was to select an effective microbial strain to improve the functional qualities of Eisenia bicyclis water extract by fermentation. For this purpose, several microorganisms isolated from traditional Korean fermented foods were inoculated and cultivated in E. bicyclis water extract. Ultimately, yeast strain YM-1 was selected for further study based on its total phenolic compound (TP) content and antioxidant activity, which were enhanced by microbial fermentation. The extract fermented by YM-1 exhibited a superior TP content and 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity compared to extracts fermented by other microbes. The highest TP content and DPPH radical scavenging activity were observed after one day of YM-1 fermentation. Yeast strain YM-1 was identified as Candida utilis based on an analysis of its physiological characteristics. During fermentation of the extract by C. utilis YM-1, no significant difference was observed in the proximate composition, including moisture, crude lipid, crude protein, and crude ash. Fermentation by C. utilis YM-1 resulted in enhanced biological activity, including increases in the TP content and antioxidant activity. Thus, fermentation by C. utilis YM-1 is an attractive strategy for developing value-added food ingredients.
Distribution of Phlorotannins in the Brown Alga Ecklonia cava and Comparison of Pretreatments for Extraction
Chowdhury, Muhammad Tanvir Hossain ; Bangoura, Issa ; Kang, Ji-Young ; Park, Nam-Gyu ; Ahn, Dong-Hyun ; Hong, Yong-Ki ;
Fisheries and aquatic sciences, volume 14, issue 3, 2011, Pages 198~204
DOI : 10.5657/FAS.2011.0198
The brown seaweed Ecklonia cava is known to be a rich source of phlorotannins that have diverse biological activities. Among the phlorotannins in E. cava, concentrations of dieckol and phlorofucofuroeckol-A, which were identified as major active components, were determined in different parts of the tissue. We compared the efficacy of different pretreatments for their extraction. A high-performance liquid chromatography (HPLC) method to determine phlorotannin concentrations showed good accuracy (92.64 and 94.02%, respectively), precision (3.92 and 3.94%, respectively), and linearity (r>0.996). Mature thalli contained 1.5-fold more dieckol (1.82 mg/g-dry tissue) than young thalli. In the tissues of E. cava, blade tissue contained more phlorotannins than the stipe or holdfast. Among differently dried thalli, approximately 90% or more dieckol and phlorofucofuroeckol-A were extracted from shadow-dried tissue as compared with lyophilized tissue. In sun-dried and oven-dried thalli, approximately 60% of the phlorotannins were extracted. Thalli washed with fresh water, boiled thalli, and steamed thalli showed reduced extraction of the compounds.
Acute Toxicity to Peptone Concentrations in the Polychaete Perinereis aibuhitensis under Laboratory Culture
Kang, Kyoung-Ho ; Zhang, Litao ; Ahn, Sam-Young ; Kahng, Hyung-Yeel ; Zhang, Zhifeng ; Sui, Zhenghong ;
Fisheries and aquatic sciences, volume 14, issue 3, 2011, Pages 205~209
DOI : 10.5657/FAS.2011.0205
Organic pollution causes eutrophication and dystrophication, which occur when excessive amounts of organic matter enters seawater. Eutrophication can contaminate sediment and harm aquaculture. Polychaeta species have been shown to restore eutrophic sediment. In this study, we used peptone to simulate a eutrophic environment and detect the levels at which eutrophication became toxic to the polychaete Perinereis aibuhitensis. Peptone concentrations were 0, 100, 200, and 500 mg/L. The median lethal concentrations were 950.35 mg/L at 48 h, 340.34 mg/L at 72 h, and 120.22 mg/L at 96 h, which are much higher than those of other aquatic species. Polychaeta species are highly tolerant of eutrophication. During the 15-day long-term experiment, sediment loss on ignition, as well as seawater total organic carbon and total nitrogen all decreased significantly (P<0.05). However,
concentration increased with time. Perinereis aibuhitensis slowed the increment of
but could not prevent its increase. Our results indicate that this polychaete is helpful in the recovery of seawater and sediment from eutrophication.
Cu Accumulation and Elimination in the Tissues of the Olive Flounder Paralichthys olivaceus
Kim, Seong-Gil ; Jang, Seok-Woo ; Lee, Young-Ju ; Kim, Seong-Soo ;
Fisheries and aquatic sciences, volume 14, issue 3, 2011, Pages 210~217
DOI : 10.5657/FAS.2011.0210
Olive flounder, Paralichthys olivaceus were exposed to waterborne Cu (control, 50, 80, 150, and 320
/L) for 30 days and then depurated for 20 days to investigate the effects of waterborne Cu exposure on growth, accumulation, and elimination. The weight-specific growth rate was significantly negatively related to waterborne Cu concentrations at 150 and 320
/L. The order of Cu accumulation in different tissues of exposed fish was liver>intestines>gills>kidneys>muscle, suggesting that the liver is more important than other tissues for the storage of Cu in olive flounder. The accumulation factor for the gills, intestines, liver, and muscle increased with increasing exposure time, and accumulation was negatively related to exposure concentration for the gills, kidneys, and muscle. Cu concentrations in the gills, intestines, and liver continuously decreased for 20 days of depuration. The fastest elimination rate occurred in the intestines at all exposure concentrations, and the order of Cu elimination in the different tissues was intestines>liver>gills.
Semiautomated Analysis of Data from an Imaging Sonar for Fish Counting, Sizing, and Tracking in a Post-Processing Application
Kang, Myoung-Hee ;
Fisheries and aquatic sciences, volume 14, issue 3, 2011, Pages 218~225
DOI : 10.5657/FAS.2011.0218
Dual frequency identification sonar (DIDSON) is an imaging sonar that has been used for numerous fisheries investigations in a diverse range of freshwater and marine environments. The main purpose of DIDSON is fish counting, fish sizing, and fish behavioral studies. DIDSON records video-quality data, so processing power for handling the vast amount of data with high speed is a priority. Therefore, a semiautomated analysis of DIDSON data for fish counting, sizing, and fish behavior in Echoview (fisheries acoustic data analysis software) was accomplished using testing data collected on the Rakaia River, New Zealand. Using this data, the methods and algorithms for background noise subtraction, image smoothing, target (fish) detection, and conversion to single targets were precisely illustrated. Verification by visualization identified the resulting targets. As a result, not only fish counts but also fish sizing information such as length, thickness, perimeter, compactness, and orientation were obtained. The alpha-beta fish tracking algorithm was employed to extract the speed, change in depth, and the distributed depth relating to fish behavior. Tail-beat pattern was depicted using the maximum intensity of all beams. This methodology can be used as a template and applied to data from BlueView two-dimensional imaging sonar.
Development of a Proficiency Test Specimen for Enumerating Escherichia coli in Molluscan Bivalve Shellfish
Kwon, Ji-Young ; Song, Ki-Cheol ; Son, Kwang-Tae ; Yu, Hong-Sik ; Kim, Poong-Ho ; Shin, Soon-Bum ; Mok, Jong-Soo ; Lee, Ka-Jeong ; Oh, Eun-Gyoung ; Yoon, Ho-Dong ; Lim, Keun-Sick ; Kim, Young-Mog ;
Fisheries and aquatic sciences, volume 14, issue 3, 2011, Pages 226~229
DOI : 10.5657/FAS.2011.0226
This study was conducted to develop a proficiency test (PT) specimen for the enumeration of Escherichia coli, a sanitary indicative bacterium, in molluscan bivalve shellfish. The common mussel Mytilus edulis was chosen as a matrix for the PT specimen. Mussels were artificially bioaccumulated for approximately 3 h with E. coli culture. After determining the homogeneity of samples, samples were distributed to 17 participants involved in the proficiency testing program. The enumeration of E. coli was performed by the most probable number method of the American Public Health Association. Statistical evaluations of the results obtained from inter- and intra-laboratory variation indicated no significant differences in the accuracy of these techniques between participants, indicating z-scores of
and suggesting that preparation of the PT specimen for enumerating E coli in shellfish was successful.
Cost-Effectiveness of Converting Fish Waste into Liquid Fertilizer
Kim, Joong-Kyun ;
Fisheries and aquatic sciences, volume 14, issue 3, 2011, Pages 230~233
DOI : 10.5657/FAS.2011.0230
To determine the cost-effectiveness of converting fish waste into liquid fertilizer, this study analyzed the production of 3 L of liquid fertilizer from the fermentation of fish waste. The total product cost of the fertilizer was calculated to be $165.26 for a one-batch operation. If the seed culture was repeated five times, the total product cost could be reduced to $36.39/L. According to this analysis, the reutilization of fish waste as liquid fertilizer was not particularly economically attractive at present, and plant-scale production would be necessary for commercialization. This is the first cost-effectiveness analysis of the bioconversion of fish waste into liquid fertilizer.