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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Fisheries and aquatic sciences
Journal Basic Information
Journal DOI :
The Korean Society of Fisheries and Aquatic Science
Editor in Chief :
Sungchul C. Bai
Volume & Issues
Volume 15, Issue 4 - Dec 2012
Volume 15, Issue 3 - Sep 2012
Volume 15, Issue 2 - Jun 2012
Volume 15, Issue 1 - Mar 2012
Selecting the target year
Isolation and Characterization of Nonylphenol-degrading Bacteria
Yu, Dae-Ung ; Kim, Dong-Myung ; Chung, Yong-Hyun ; Lee, Yang-Bong ; Kim, Young-Mog ;
Fisheries and aquatic sciences, volume 15, issue 2, 2012, Pages 91~97
DOI : 10.5657/FAS.2012.0091
To isolate a nonylphenol (NP)-degrading bacterium, we isolated a single colony from the NP-degrading microbial consortium SW-3, which was previously isolated from an aqueous environment. Ten colonies that exhibited different cell morphologies were isolated and the strains were named SW-3-A, -B, -C, -D, -E, -F1, -F2, -G, -H, and -I. The ability of isolates to degrade NP was evaluated by kinetic analysis by the constant of NP degradation rate (
) and the half-life time of NP degradation (
). SW-3-F1, -F2, -G, and -I strains were superior at degrading NP. The
values of the four strains were sixfold higher and one-sixth lower, respectively, than those of the consortium strain. Additionally, SW-3-F1, -G, and -I strains were tested for their ability to degrade NP during coculture. NP degradation by coculture with a combination of all three strains was inferior to that of culture conducted with single isolates, suggesting that the three strains are antagonistic toward each other during NP degradation.
Biochemical Composition of Muscle from Tanaka's Eelpout Lycodes tanakae, Magistrate Armhook Squid Berryteuthis magister, and Ocean Sunfish Mola mola, Caught in the East Sea, Korea
Lee, Doo-Seog ; Cho, Hyeon-Ah ; Yoon, Na-Young ; Kim, Yeon-Kye ; Lim, Chi-Won ; Shim, Kil-Bo ;
Fisheries and aquatic sciences, volume 15, issue 2, 2012, Pages 99~105
DOI : 10.5657/FAS.2012.0099
The biochemical composition of muscle from three deep-sea animals, Tanaka's eelpout Lycodes tanakae magistrate armhook squid Berryteuthis magister, and ocean sunfish Mola mola caught in the East sea in South Korea was determined. The moisture (81.4-93.8 g/100 g), crude protein (5.0-15.6 g/100 g), crude lipid (0.1-1.5 g/100 g), and ash (1.0-1.1 g/100 g) contents of the two fishes were analyzed. The proximate composition of magistrate armhook squid was: moisture 84.0 g/100 g, crude protein 12.4 g/100 g, crude lipid 0.7 g/100 g, and ash 1.5 g/100 g. There was little difference in the proximate compositions of the three animals. The total amino acid contents of Tanaka's eelpout, magistrate armhook squid, and ocean sunfish were 14.64, 10.75, 3.10-9.95 g/100 g, respectively. High levels of glutamic acid, aspartic acid, lysine, leucine, and arginine were found, while low levels of histidine, glycine, and cysteine were detected in the animals. A survey of the free amino acid contents of the animals revealed large amounts of alanine and glycine. Significant differences were found in the fatty acid composition among the three species. Specially, different saturated fatty acids (17.14-40.49%) such as C16:0, and C18:0, monounsaturated fatty acids (19.19-46.88%) such as C16:1, and C18:1, and polyunsaturated fatty acids (35.41-57.08%) such as EPA, and DHA was identified. Our results suggest that each of these deep sea animals possesses nutritional value and should be considered as a foodstuff.
Development of a Denaturing High-Performance Liquid Chromatography (DHPLC) Assay to Detect Parasite Infection in Grass Shrimp Palaemonetes pugio
Cho, Sang-Man ;
Fisheries and aquatic sciences, volume 15, issue 2, 2012, Pages 107~115
DOI : 10.5657/FAS.2012.0107
In developing a useful tool to detect parasitic dynamics in an estuarine ecosystem, a denaturing high-performance liquid chromatography (DHPLC) assay was optimized by cloning plasmid DNA from the grass shrimp Palaemonetes pugio, and its two parasites, the trematode Microphallus turgidus and bopyrid isopod Probopyrus pandalicola. The optimal separation condition was an oven temperature of
and 62-68% of buffer B gradient at a flow rate of 0.45 mL/min. A peptide nucleic acid blocking probe was designed to clamp the amplification of the host gene, which increased the amplification efficiency of genes with low copy numbers. Using the DHPLC assay with wild-type genomic, the assay could detect GC Gram positive bacteria and the bopyrid isopod (P. pandalicola). Therefore, the DHPLC assay is an effective tool for surveying parasitic dynamics in an estuarine ecosystem.
Morphological Specificity in Cultured Starry Flounder Platichthys stellatus Reared in Artificial Facility
Kang, Duk-Young ; Lee, Jong-Ha ; Kim, Won-Jin ; Kim, Hyo-Chan ;
Fisheries and aquatic sciences, volume 15, issue 2, 2012, Pages 117~123
DOI : 10.5657/FAS.2012.0117
The starry flounder Platichthys stellatus, like all flatfish, exhibits conspicuous lateral asymmetry in numerous traits, most obvious of which is the migration of one eye to the other side of the head during metamorphosis. Additional changes related to eye migration include asymmetrical pigmentation, and a behavioral shift from larvae that exhibit upright, open-water swimming to juveniles and adults that lie on the ocean floor, eye side up. However, the morphology of these juveniles has been quite plastic in recent years, a phenomenon which is thought to be related to a diverse suite of semi-intensive and intensive larviculture methods. The cause of morphological abnormalities in the farmed flatfish is poorly understood. In the present study, we observe the features of morphological specificity and abnormality of immature fish (mean total length 23 cm) and survey the occurrence frequency of the specificity and abnormality of juvenile (mean total length 6.70 cm) in artificial culture facility. We find 2 types of abnormality (e.g., albino in ocular side and hypermelanosis in blind side) and 1 type of specificity (e.g., lateral polymorphism). These considerably differ from normal individuals (has sinistral eye and pigmented on only one side) by several characteristics (dextral eye, ocular side albinism, blind side hypermelanosis). The incidences of albinism, hypermelanosis, and body reversal are
, respectively. These suggest that these morphometric and morphological differences occur more in artificial environment during and just after metamorphosis.
Comparison Between Phylogenetic Relationships Based on 18S rDNA Sequences and Growth by Salinity of Chlorella-like Species (Chlorophyta)
Lee, Hye-Jung ; Hur, Sung-Bum ;
Fisheries and aquatic sciences, volume 15, issue 2, 2012, Pages 125~135
DOI : 10.5657/FAS.2012.0125
This study was carried out to understand the correlation between phylogenetic relationships based on 18S rDNA sequences and growth by salinity of Chlorella-like species. The 18S rDNA sequences of 71 Chlorella-like species which were mainly collected from Korean waters were analyzed. The 18S rDNA sequences of Chlorella-like species were divided into three groups (group A, B and C) and group B was further divided into three subgroups (subgroup B-1, B-2 and B-3). Thirty-seven Chlorella-like species in group A grew well at high salinity (32 psu) but the other groups grew well in freshwater. The sequence identities of the species in group A and B were 97.2-99.5%, but those of 6 species in group C ("Chlorella" saccharophila), which contained group I intron sequences region were 75.0-75.4%. Two representative species of each group were cultured at different salinities (0, 16 and 32 psu) to examine the correlation between the molecular phylogenetic groups and the phenotypic characteristics on cell growth and size by different salinities. The size of cell cultured at different salinities varied according to the species of each molecular phylogenetic group. The size of "Chlorella" saccharophila in group C was bigger and more obviously elliptical rather than that of the other Chlorella-like species. Considering the results on molecular and phenotypic characteristics, the group A and B belonged to Chlorellaceae, but group C was distinctly different from them.
Abalone Haliotis discus hannai Intestine Digests with Different Molecule Weights Inhibit MMP-2 and MMP-9 Expression in Human Fibrosarcoma Cells
Nguyen, Van-Tinh ; Qian, Zhong-Ji ; Jung, Won-Kyo ;
Fisheries and aquatic sciences, volume 15, issue 2, 2012, Pages 137~143
DOI : 10.5657/FAS.2012.0137
The abalone Haliotis discus hannai, is one of the economically important species in the fisheries industry. Abalone intestines are one of the by-products of its processing. To investigate its bioactive potential, abalone intestine was digested using an in vitro gastrointestinal (GI) digestion system containing pepsin, trypsin, and
-chymotrypsin. The abalone intestine G1 digests (AIGIDs) produced by the GI digestion system were fractionated into AIGID I (> 100 kDa), AIGID II (10-100 kDa), and AIGID III (1-10 kDa) using an ultrafiltration membrane system. Of the three digests, AIGID II and AIGID III exhibited inhibitory effects against matrix metalloproteinase-2 and -9 (MMP-2, MMP-9) in HT1080 human fibrosarcoma cells. Both fractions potently inhibited gelatine digestion by MMP-2 and MMP-9 treated with phorbol 12-myristate 13-acetate (PMA) and migration of HT1080 cells in dose dependently. Furthermore, AIGID II and III attenuated expression of p65, a component of nuclear transcription factor kappa B. These results indicate that of the abalone intestine digests inhibit MMP-2 and MMP-9. Thus, the AIGIDs or their active components may have preventive and therapeutic potential for diseases associated with MMP-2 and MMP-9 activation in fibrosarcoma cells.
Selection of a L-Lysine-Overproducing Strain of the Red Seaweed Porphyra suborbiculata (Rhodophyta) through Mutation and Analog Enrichment
Luyen, Quoc-Hai ; Chowdhury, Muhammad Tanvir Hossain ; Choi, Jae-Suk ; Kang, Ji-Young ; Park, Nam-Gyu ; Hong, Yong-Ki ;
Fisheries and aquatic sciences, volume 15, issue 2, 2012, Pages 145~150
DOI : 10.5657/FAS.2012.0145
An improved strain of the red seaweed Porphyra suborbiculata containing an increased amount of the essential amino acid L-lysine was obtained through mutation and analog enrichment. Mutagenesis using a 10% lethal dose of ultraviolet irradiation and an enrichment culture with the L-lysine analog aminoethyl-L-cysteine (AEC) was repeated to select the most productive strain using monospores of P. suborbiculata. The concentrations of AEC required to produce 50 and 100% inhibition of survival were 60 and 115 mM in the parent strain, and 72 and 135 mM in the selected AEC-resistant strain, respectively. The AEC-resistant strain, L130, produced 1.74-fold more lysine compared to its parent strain. Thus, mutagenesis with analog enrichment shows promise for selecting seaweed strains that can overproduce this essential amino acid.
Genetic Variation in Wild and Cultured Populations of the Sea Squirt Halocynthia roretzi Inferred from Microsatellite DNA Analysis
Han, Hyon-Sob ; Nam, Bo-Hye ; Kang, Jung-Ha ; Kim, Yi-Kyoung ; Jee, Young-Ju ; Hur, Young-Baek ; Yoon, Moon-Geun ;
Fisheries and aquatic sciences, volume 15, issue 2, 2012, Pages 151~155
DOI : 10.5657/FAS.2012.0151
We used nine microsatellite DNA markers to estimate genetic variation among wild and cultured populations of the sea squirt Halocynthia roretzi. The loci were polymorphic, with 6-32 alleles, and allelic richness ranged from 6.0 to 26.1 in each population. The wild and the cultured populations had similar mean heterozygosities (
), allele numbers, and allelic richness. One cultured population with softness syndrome had a lower mean in the observed heterozygosity (
= 0.57) and higher mean inbreeding coefficient (
= 0.261) than any other populations. This suggests that the loss of genetic variation in the diseased population might be due to increased inbreeding. A neighbor-joining tree and pairwise population estimates of
showed moderate genetic differentiation between the wild and the cultured populations. Additionally, the softness syndrome population was genetically divergent from wild populations, but it was genetically close to the cultured populations.
Detection of Laminariaceae Species Based on PCR by Family-specific ITS Primers
Choi, Chang-Geun ; Kim, Jong-Myoung ;
Fisheries and aquatic sciences, volume 15, issue 2, 2012, Pages 157~162
DOI : 10.5657/FAS.2012.0157
To analyze nucleotide sequence encoding internal transcribed spacer (ITS) regions specific to the Laminariaceae family, genomic DNA was isolated from six brown algae species distributed along the east coast of Korea. These included three species from the Laminariaceae family (Agarum clathratum Dumortier, Costaria costata [C. Agardh] Saunders, and Saccharina japonica Areschoug) and two species from the Alariaceae family (Undaria pinnatifida [Harvey] Suringer and Ecklonia cava Kjellman), both in the order Laminariales, and one species from the family Sargassaceae in the order Fucales (Sargassum serratifolium). Based on a sequence analysis of ITS-1 and ITS-2 for A. clathratum, C. costata, and E. cava, oligonucleotides were designed from the regions that showed sequence conservation in Laminariaceae. Following polymerase chain reaction using three sets of primers, amplification of ITS-1 and ITS-2 was detected in reactions using genomic DNA isolated from the species belonging to Laminariaceae, but not from the species belonging to the other families. The results indicate that this method can be used for the detection and identification of Laminariaceae species.
Optimal Conditions for the Post-Harvest Storage of Rhizoids of the Brown Seaweed Undaria pinnatifida (Phaeophyta) for Arachidonic Acid Production
Khan, Mohammed Nurul Absar ; Kang, Ji-Young ; Park, Nam-Gyu ; Choi, Jae-Suk ; Cho, In-Soon ; Hong, Yong-Ki ;
Fisheries and aquatic sciences, volume 15, issue 2, 2012, Pages 163~168
DOI : 10.5657/FAS.2012.0163
The non-utilized biomass of the aquacultured seaweed Undaria pinnatifida, particularly the rhizoid, is an alternative source of arachidonic acid (AA). Of the five aquacultured kelps that were tested, U. pinnatifida yielded the highest amount of AA, which was isolated from the rhizoids. Its identity (C20:4 n-6) was confirmed from gas chromatography-mass spectrometry spectral data. The optimal conditions for post-harvest storage or pretreatment of the rhizoids in Provasoli's enriched seawater for AA extraction were determined to be pH 7.8, 2%
-enriched air, 20
. Under these conditions, the AA content after 1 day of storage was enhanced by up to 127%. In the absence of light under ambient aeration, the AA content after 1 day of storage diminished to 90%. Rhizoids collected late in the season (April and May) contained the highest amounts of AA (approximately 2.5 mg/g tissue).
Age and Growth of Blackfin Flounder Glyptocephalus stelleri in the East Sea, Korea
Yang, Jae-Hyeong ; Yoon, Sang-Chul ; Lee, Sung-Il ; Cha, Hyung-Kee ; Kim, Jong-Bin ; Choi, Young-Min ; Park, Jeong-Ho ;
Fisheries and aquatic sciences, volume 15, issue 2, 2012, Pages 169~176
DOI : 10.5657/FAS.2012.0169
Age and growth of Glyptocephalus stelleri in the East Sea of Korea were determined, from monthly samples of commercial catches, caught by the eastern sea Danish seine fishery in 2007. The annuli of G. stelleri are formed once a year, with the boundary between opaque and translucent zones forming in September. Relationships between total length (TL) and total weight (TW) were
) for females and
) for males. TLs at annuli formation in otoliths were back-calculated from the otolith-length relationship and were adjusted to von Bertalanffy growth curves to
for females and
for males. From the age of 3 years, females grew faster than males (P<0.05).
Effects of pH Change by CO
Induction and Salinity on the Hatching Rate of Artemia franciscana
Salma, Umme ; Uddowla, Md. Hasan ; Lee, Gi-Hun ; Yeo, Young-Min ; Kim, Hyun-Woo ;
Fisheries and aquatic sciences, volume 15, issue 2, 2012, Pages 177~181
DOI : 10.5657/FAS.2012.0177
To understand the effects of lower pH levels due to elevated
and salinity, we designed and constructed a pH-control system that included automatic
infusion and measured the hatching rate of a crustacean model species, Artemia franciscana. The pH-control system was cost-effective and capable of performing animal tests in which pH fluctuated around
, with the temperature around
. Hatching rate was observed under four different pH levels (7.0, 7.3, 7.6, and untreated control) combined with three salinity ranges (15, 25, and 35 ppt). The results demonstrated that lower pH levels led to decreased hatching rates regardless of salinity, and the minimum hatching rate was detected at pH 7.0 compared to the control (pH
), supporting the idea that OA has adverse effects on hatching rates and increases the risk of juveniles being introduced in the ecosystem. In contrast, salinity changes exhibited no synergistic effects with pH and had independent effects.