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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Fisheries and aquatic sciences
Journal Basic Information
Journal DOI :
The Korean Society of Fisheries and Aquatic Science
Editor in Chief :
Sungchul C. Bai
Volume & Issues
Volume 15, Issue 4 - Dec 2012
Volume 15, Issue 3 - Sep 2012
Volume 15, Issue 2 - Jun 2012
Volume 15, Issue 1 - Mar 2012
Selecting the target year
The Novel Angiotensin I Converting Enzyme Inhibitory Peptide from Rainbow Trout Muscle Hydrolysate
Kim, Sung-Rae ; Byun, Hee-Guk ;
Fisheries and aquatic sciences, volume 15, issue 3, 2012, Pages 183~190
DOI : 10.5657/FAS.2012.0183
The purpose of this study was the purification and characterization of an angiotensin I converting enzyme (ACE) inhibitory peptide purified from enzymatic hydrolysates of rainbow trout Oncorhynchus mykiss muscle. After removal of lipid, the approximate composition analysis of the rainbow trout revealed 24.4%, 1.7%, and 68.3% for protein, lipid, and moisture, respectively. Among six hydrolysates, the peptic hydrolysate exhibited the highest ACE inhibitory activity. We attempted to purify ACE inhibitory peptides from peptic hydrolysate using high performance liquid chromatography on an ODS column. The
value of purified ACE inhibitory peptide was
. The amino acid sequence of the peptide was identified as Lys-Val-Asn-Gly-Pro-Ala-Met-Ser-Pro-Asn-Ala-Asn, with a molecular weight of 1,220 Da, and the Lineweaver-Burk plots suggested that they act as a competitive inhibitor against ACE. Our study suggested that novel ACE inhibitory peptides purified from rainbow trout muscle protein may be beneficial as anti-hypertension compounds in functional foods.
Isolation of Fucosterol from Pelvetia siliquosa by High-speed Countercurrent Chromatography
Hwang, Seung Hwan ; Jang, Jai Man ; Lim, Soon Sung ;
Fisheries and aquatic sciences, volume 15, issue 3, 2012, Pages 191~195
DOI : 10.5657/FAS.2012.0191
We report here the use of high-speed countercurrent chromatography (HSCCC) in the preparative isolation and purification of the bioactive component, fucosterol, from Pelvetia siliquosa. A crude extract was obtained by ultrasonic extraction of powdered P. siliquosa using methylene chloride and was then subjected to separation and purification by HSCCC, coupled with evaporative light-scattering detection. Preparative HSCCC was performed successfully using a two-phase solvent system, n-heptane:methanol (3:2, v/v), to obtain 10.96 mg fucosterol with 96.8% purity from 50 mg of crude extract; the recovery rate was approximately 90.5%.
Endoprotease and Exopeptidase Activities in the Hepatopancreas of the Cuttlefish Sepia officinalis, the Squid Todarodes pacificus, and the Octopus Octopus vulgaris Cuvier
Kim, Min Ji ; Kim, Hyeon Jeong ; Kim, Ki Hyun ; Heu, Min Soo ; Kim, Jin-Soo ;
Fisheries and aquatic sciences, volume 15, issue 3, 2012, Pages 197~202
DOI : 10.5657/FAS.2012.0197
This study examined and compared the exopeptidase and endoprotease activities of the hepatopancreas (HP) of cuttlefish, squid, and octopus species. The protein concentration in crude extract (CE) from octopus HP was 3,940 mg/100 g, lower than those in CEs from squid HP (4,157 mg/100 g) and cuttlefish HP (5,940 mg/100 g). With azocasein of pH 6 as a substrate, the total activity in HP CE of octopus was 31,000 U, lower than the values for cuttlefish (57,000 U) and squid (69,000 U). Regardless of sample type, the total activities of the CEs with azocasein as the substrate were higher at pH 6 (31,000-69,000 U) than at pH 9 (19,000-34,000 U). With L-leucine-p-nitroanilide (LeuPNA) of pH 6 as the substrate, the total activity of the HP CE from octopus was 138,000 U, higher than values from both cuttlefish HP (72,000 U) and squid HP (63,000 U). Regardless of sample type, the total activities of the CEs with LeuPNA as the substrate were higher at pH 6 (63,000-138,000 U) than at pH 9 (41,000-122,000 U). With LeuPNA as the substrate, the total activities of the CEs from octopus HP and cuttlefish HP were higher at pH 6 than at pH 9. However, there was no difference in total activity between pH 6 and 9 for squid HP CE with LeuPNA as the substrate. These results suggest that the octopus HP is superior to the cuttlefish HP and squid HP as a potential resource for extracting exopeptidases. Exopeptidases from octopus HP have potential industrial applications and their use might aid in reducing pollution related to the octopus industry.
Organic Acid Extraction of Fluoride from Antarctic Krill Euphausia superba
Xie, Cheng Liang ; Kim, Han Soo ; Shim, Kil Bo ; Kim, Yeon Kye ; Yoon, Na Yeong ; Kim, Poong Ho ; Yoon, Ho Dong ;
Fisheries and aquatic sciences, volume 15, issue 3, 2012, Pages 203~207
DOI : 10.5657/FAS.2012.0203
The amount of fluoride removed from Antarctic krill via organic acid extraction depends on the extraction time, concentration, extraction volume, and the nature of the acid itself. The fluoride content in Euphausia superba was determined by measuring the concentration of fluoride ion in acidic extracts using an ion-selective electrode. The best results were obtained by adding 50 mL of 0.01 M citric acid to 0.3-0.5 g of krill and extracting for 5 min. Under these conditions, recoveries of fluoride from frozen whole krill and krill meat were 95.6-99.5% and 97.5-101.3%, respectively. The dry basis fluoride contents of krill by-product, krill meat and the boiled krill were 705, 298, and 575 ppm, respectively. These levels were significantly reduced by citric acid extraction.
Biological Application of Two Protozoan Species, Euplotes sp. and Vorticella sp., for the Stable Culture of the Rotifer Brachionus rotundiformis in Laboratory Experiments of Inter- and Tripartite-Specific Relations
Jung, Min-Min ;
Fisheries and aquatic sciences, volume 15, issue 3, 2012, Pages 209~213
DOI : 10.5657/FAS.2012.0209
Members of the ciliate group of protozoans are often observed in mass cultures of rotifers. In particular, Euplotes and Vorticella are common contaminating species. In this study, I examined the effect of the ciliates Euplotes sp. and Vorticella sp. on the growth of the rotifer Brachionus rotundiformis by conducting inter-specific and tripartite-specific mixed-culture experiments. The growth of rotifers was suppressed in co-existence with Euplotes sp. compared with monocultures of rotifers. However, Vorticella sp. promoted rotifer growth. Moreover, Vorticella sp. improved the growth of rotifers suppressed by Euplotes sp. contaminants. In 5-L semi-mass cultures of rotifers, growth of the contaminating protozoan Euplotes sp. was heavily suppressed by Vorticella sp. The stable maintenance of the rotifer culture ecosystem can be achieved by manipulating the types of contaminating protozoan species.
Effect of Inorganic Mercury on Hematological and Antioxidant Parameters on Olive Flounder Paralichthys olivaceus
Kim, Jun-Hwan ; Lee, Jung-Sick ; Kang, Ju-Chan ;
Fisheries and aquatic sciences, volume 15, issue 3, 2012, Pages 215~220
DOI : 10.5657/FAS.2012.0215
The effects of inorganic mercury on hematological parameters and hepatic oxidative stress enzyme activity were studied in olive flounder Paralichthys olivaceus. Fish were injected twice intraperitoneally with mercuric chloride (2, 4, or 8 mg Hg/kg BW). The major hematological findings were significant decreases in the red blood cell count, hematocrit value, and hemoglobin level in olive flounder exposed to 8 mg Hg/kg BW. Remarkably low levels of calcium and chloride, and reduced osmolality, were also observed at 8 mg Hg/kg BW. In hepatic tissue, significant increases in glutathione peroxidase and catalase activity were observed above 4 mg Hg/kg BW Inorganic mercury also increased glutathione S-transferase and glutathione reductase activity at 8 mg Hg/kg BW in hepatic tissue. The present findings suggest that exposure to a low concentration (
mg Hg/kg BW) of inorganic mercury can cause significant changes in hematological and antioxidant parameters.
Gene Structure and Estrogen-Responsive mRNA Expression of a Novel Choriogenin H Isoform from a Marine Medaka Oryzias dancena
Lee, Sang Yoon ; Kim, Dong Soo ; Nam, Yoon Kwon ;
Fisheries and aquatic sciences, volume 15, issue 3, 2012, Pages 221~231
DOI : 10.5657/FAS.2012.0221
The marine medaka Oryzias dancena choriogenin H gene (odChgH) and its mRNA expression during estradiol-
(E2) exposure were characterized. At the amino acid level, the choriogenin H protein is predicted to possess the conserved repetitive N-terminal region, as well as zona pellucida (ZP) and Trefoil factor family (TFF) domains. At the genomic level, odChgH has an eight-exon organization with a distribution pattern of transcription factor binding sites in the 5'-upstream region, which is commonly found in other estrogen-responsive genes. The tissue distribution pattern of odChgH mRNA was found to be gender-specific, whereby females showed a higher expression level and wider tissue distribution than did males. During embryonic development, odChgH mRNA was robustly detected from the stage of visceral blood vessel formation. Experimental E2 exposure of males resulted in odChgH mRNA being induced not only in the liver, but also in other several tissues. The E2-mediated induction was fairly dose-dependent. The basal expression levels of hepatic odChgH mRNA were lower in males that were acclimated to 30 ppt salinity than in those acclimated to 0 or 15 ppt salinity. In contrast, the inducibility of odChgH mRNA during E2 exposure was greater in seawater-acclimated fish than in brackish water- or freshwater-acclimated fish.
Clinical, Hematological, and Biochemical Alterations in Olive Flounder Paralichthys olivaceus Following Experimental Infection by Vibrio scophthalmi
Qiao, Guo ; Park, Soo Il ; Xu, De-Hai ;
Fisheries and aquatic sciences, volume 15, issue 3, 2012, Pages 233~239
DOI : 10.5657/FAS.2012.0233
Hematological analysis can provide crucial information for monitoring the health of fish. However, there is no current information available regarding hematological changes in olive flounder following infection by Vibrio scophthalmi. In this study, hematological and biochemical alterations were determined in olive flounder infected by the high virulence strain (HVS) and low virulence strain (LVS) of V. scophthalmi. Survival in serum, skin mucus, and macrophages of olive flounder was also compared between the HVS and LVS. The results demonstrated that the hematocrit value in infected fish declined from 23.4% at 0 h to 18.0% at 168 h post infection. The total protein concentration in fish infected with the HVS was significantly higher than in fish infected with the LVS and a non-infected control. Lysozyme activity was significantly different between infected and control fish. The HVS survived in serum and cell numbers increased substantially, while cell numbers of the LVS in serum decreased. These changes in hematological characteristics in fish infected by V. scophthalmi can be used as an effective and sensitive index to monitor the physiological and pathological conditions of fish. The survival and reproduction of V. scophthalmi in host serum, skin mucus, and macrophages play a major role in systemic infection and can serve as a virulence indicator for different strains.
Chemical Contamination and Toxicity of Sediments from the Gunsan Coast, Korea
Lee, Wan-Seok ; Choi, Minkyu ; Hwang, Dong-Woon ; Lee, In-Seok ; Kim, Sook Yang ;
Fisheries and aquatic sciences, volume 15, issue 3, 2012, Pages 241~250
DOI : 10.5657/FAS.2012.0241
Polycyclic aromatic hydrocarbons (PAHs), butyltins (BTs), nonylphenol (NP), and fecal sterols concentrations in sediments were investigated from Gunsan coast of Korea to evaluate organic pollution from anthropogenic activities. Sediment toxicity was also examined by bacterial bioluminescence toxicity test (Vibrio fischeri). The concentrations of 16 PAHs in sediments ranged from 67.9 to 425 ng/g dry wt; BTs ranged from 2.79 to 14.1 ng Sn/g dry wt; NP ranged from 20.7 to 2171 ng/g dry wt; and coprostanol, a fecal sterol, ranged from 7.60 to 245 ng/g dry wt. Effective concentration 50% (
) of sediments ranged from 0.38 to 23.8 mg/mL. Most of the chemicals were present at levels lower than or comparable to the previously reported values from Korea. However, NP levels in the present study were in the high range of levels reported from the Korean coast, and 40% of the measured samples exceeded screening and ecotoxicological values of NP suggested by the Netherlands and Canada. This suggests that an ongoing source of NP is a serious concern in the Gunsan coast. High levels of contaminants were found in the proximity of potential sources, such as the outfall of a wastewater treatment plant for NP, an anthracite-fired power plant for PAHs, and ports for BTs, fecal sterols, and sediment toxicity. This indicates that Gunsan coast has various potential sources of marine sediment contaminants.
First Record of the Black Snoek Thyrsitoides marleyi (Pisces: Gempylidae) from Korea
Kim, Maeng Jin ; Choi, Jung Hwa ; Kim, Jung Nyun ; Oh, Taeg Yun ; Lee, Dong Woo ;
Fisheries and aquatic sciences, volume 15, issue 3, 2012, Pages 251~253
DOI : 10.5657/FAS.2012.0251
A single specimen of Thyrsitoides marleyi (818 mm in standard length), belonging to the family Gempylidae, was collected for the first time in Korea from the coastal waters off Busan, Korea using longline with hooks on 27 January 2012. This species is characterized by the following morphological traits: palate with three fangs, dark dorsal spine membrane, ending part of soft dorsal and anal fin rays finlet-like, and tip of the jaw with a cartilaginous process. We suggest a new Korean name "Gal-chi-ggo-chi" for T. marleyi.
Stylocheiron abbreviatum (Euphausiacea, Crustacea): A New Record from the Korean Waters
Suh, Hae-Lip ;
Fisheries and aquatic sciences, volume 15, issue 3, 2012, Pages 255~257
DOI : 10.5657/FAS.2012.0255
The euphausiid crustacean Stylocheiron abbreviatum G. O. Sars, 1883 has been found in the southern waters of Jeju Island, Korea. This species is the third member of the genus Stylocheiron and the twelfth of the family Euphausiidae identified in Korean waters. Using descriptions of this species, a key to Korean euphausiids is presented.
Isolation and Characterization of Novel Alginate-Degrading Pseudoalteromonas sp. Y-4
Cho, Hyeon-Ah ; Kim, Hyun-Woo ; Kim, Young-Mog ;
Fisheries and aquatic sciences, volume 15, issue 3, 2012, Pages 259~263
DOI : 10.5657/FAS.2012.0259
To isolate an alginate-degrading bacterium, we conducted a single colony isolation using a solid medium containing alginate as the sole carbon source. A marine bacterium Y-4 capable of degrading alginate was isolated from seawater. The strain was identified to be Pseudoalteromonas sp., based on morphological, biochemical, 16S rDNA homology, and phylogenetic analyses. Moreover, Pseudoalteromonas sp. Y-4 exhibited alginate lyase activity in the presence of 4% alginate even though many known alginate-degrading bacteria degrade in the range of 0.5-1% alginate. The optimum culture conditions for the Y-4 strain were 2% alginate, pH 8.0, and 3% NaCl at
. The highest alginate lyase activity was also observed under the same conditions. To our knowledge, this is the first reported isolation of a marine bacterium degrading high concentrations of alginate.