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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Fisheries and aquatic sciences
Journal Basic Information
Journal DOI :
The Korean Society of Fisheries and Aquatic Science
Editor in Chief :
Sungchul C. Bai
Volume & Issues
Volume 16, Issue 4 - Dec 2013
Volume 16, Issue 3 - Sep 2013
Volume 16, Issue 2 - Jun 2013
Volume 16, Issue 1 - Mar 2013
Selecting the target year
Changes in the Free Amino Acid Content of the Shucked Oyster Crassostrea gigas Stored in Salt Water at 3℃
Tanimoto, Shota ; Kawakami, Koji ; Morimoto, Satoshi ;
Fisheries and aquatic sciences, volume 16, issue 2, 2013, Pages 63~69
DOI : 10.5657/FAS.2013.0063
Shucked oysters were soaked in an equal weight of salt water and stored at
for 7 days. Changes in the free amino acid content of the whole body and in the adductor muscle were evaluated by a practical distribution method. With the exception of aspartic acid and tyrosine, no significant changes in free amino acids or ammonia were observed in whole-body shucked oysters during the storage period. In contrast, the majority of free amino acids in the adductor muscle decreased significantly. Most of these free amino acids were detected in considerable amounts in the surrounding salt water after 7 days of storage. Both the weight of the whole body and the salinity of the surrounding salt water decreased significantly during the storage period. These results suggest that free amino acids were eluted from the cutting surface of the adductor muscle and indicate that the free amino acid content per shucked oyster and in the adductor muscle, decreases during cold storage.
Stability of Antioxidant Properties and Essential Amino Acids in Squid Viscera Hydrolysate Produced using Subcritical Water
Kim, Ryoung-Hee ; Asaduzzaman, A.K.M. ; You, Chi-Hong ; Chun, Byung-Soo ;
Fisheries and aquatic sciences, volume 16, issue 2, 2013, Pages 71~78
DOI : 10.5657/FAS.2013.0071
Subcritical water hydrolysis was carried out in a batch reactor to produce valuable materials, such as low-molecular weight (MW) peptides and essential amino acids with antioxidant properties, from heat-dried squid viscera. Hydrolysis of squid viscera was performed at 160 to
for 3 min. The yield was increased by increasing the temperature and pressure, while the protein content of squid viscera hydrolysate decreased with increasing temperature. Low-MW peptides were detected in all hydrolysates by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The highest yields of free and structural amino acids in heat-dried squid viscera hydrolysate were at
mg/100 g, respectively. All essential amino acids were detected in viscera hydrolysates; leucine was the most abundant. Antioxidant activities of hydrolysates were highest at
. Greater than
of the ABTS antioxidant activity was retained in hydrolysates after long-term heat treatment.
Antibacterial Activity of an Ethyl Acetate Extract of Pseudomonas sp. UJ-6 against Methicillin-Resistant Staphylococcus aureus
Lee, Dae-Sung ; Eom, Sung-Hwan ; Je, Jae-Young ; Kim, Young-Mog ; Lee, Myung-Suk ; Kim, Young-Man ;
Fisheries and aquatic sciences, volume 16, issue 2, 2013, Pages 79~84
DOI : 10.5657/FAS.2013.0079
In an effort to discover an alternative antibiotic for treating infections with methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas sp. UJ-6, a marine bacterium that exhibited antibacterial activity against MRSA, was isolated. The culture broth and its ethyl acetate extract exhibited bactericidal activity against MRSA. The extract also exhibited antibacterial activity against gram-negative bacteria, which were not susceptible to vancomycin. The treatment of MRSA with the extract resulted in abnormal cell lysis. The extract retained >95% of its anti-MRSA activity after heat treatment for 15 min at
. Thus, although most antibiotics are unstable under conditions of thermal stress, Pseudomonas sp. UJ-6 produces a heat-stable anti-MRSA substance. The results of this study strongly suggest that Pseudomonas sp. UJ-6 can be used to develop a novel, heat-stable, broad-spectrum antibiotic for the treatment of MRSA infections.
Comparison of Major Nutrients in Eels Anguilla japonica Cultured with Different Formula Feeds or at Different Farms
Seo, Jae-Sung ; Choi, Jae-Hee ; Seo, Ji-Hun ; Ahn, Tae-Ho ; Chong, Won-Seog ; Kim, Seung-Hoon ; Cho, Hye-Sun ; Ahn, Jun-Cheul ;
Fisheries and aquatic sciences, volume 16, issue 2, 2013, Pages 85~92
DOI : 10.5657/FAS.2013.0085
To determine the relative importance of two main factors, diet or culture environment, that affect the proximate composition and main nutritive ingredients (vitamin A, vitamin E, cholesterol, fatty acid composition) in cultured eels, we analyzed the composition of eels fed diets of formula feed (FF) produced by four different companies and of eels cultured at five different eel farms that provided only one of the four different FFs. The four commercial eel FFs did not markedly differ in proximate composition or major nutritive compounds, and consequently, these variables did not significantly differ in cultured eels fed the different FFs. The FF imported from Japan was marginally superior to the two domestic commercial FFs and the FF imported from Taiwan in terms of the proximate composition and main nutritive ingredients of both the FF itself and the eels cultured on it. However, proximate composition and main nutritive ingredients significantly differed among eels cultured at the five farms that used a different FF and among eels fed the four different FFs at the same farm. In conclusion, the difference in quality between domestic and Japanese FFs in terms of eel culture was small, whereas physical or chemical environmental differences among farms during eel culture may more strongly affect the proximate composition and levels of the main nutritive ingredients in cultured eels.
The First Report of a Megalocytivirus Infection in Farmed Starry Flounder, Platichthys stellatus, in Korea
Won, Kyoung-Mi ; Cho, Mi Young ; Park, Myoung Ae ; Jee, Bo Young ; Myeong, Jeong-In ; Kim, Jin Woo ;
Fisheries and aquatic sciences, volume 16, issue 2, 2013, Pages 93~99
DOI : 10.5657/FAS.2013.0093
In 2009, a systemic megalocytivirus infection associated with high mortality was detected for the first time in cultured starry flounder Platichthys stellatus in Korea. Diseased starry flounder had pale bodies and gill coloring and enlarged spleens. Histopathological examinations revealed basophilic enlarged cells in various organs of diseased starry flounder. Polymerase chain reaction (PCR) was performed on tissue samples using three published primer sets developed for the red sea bream iridovirus. PCR products were detected for all primer sets, except 1-F/1-R, which are registered by the World Organization for Animal Health (OIE). The part of the gene corresponding to the full open reading frame encoding the viral major capsid protein (MCP) was amplified by PCR. PCR products of approximately 1,581 bp were cloned, and the nucleotide sequences were analyzed phylogenetically. The MCP gene of the starry flounder iridovirus, designated SFIV0909, was identical to that of the turbot reddish body iridovirus (AB166788).
Fluttering Characteristics of the Ropes and Nets as an Active Stimulating Device inside the Cod End of a Trawl Net
Kim, Yong-Hae ;
Fisheries and aquatic sciences, volume 16, issue 2, 2013, Pages 101~108
DOI : 10.5657/FAS.2013.0101
An active stimulating device (ASD) consisting of a net panel or ropes fluttering in the turbulence inside the cod end was effective in driving fish near the cod end to reduce juvenile by-catch. The fluttering characteristics of the rope and net panel were examined by video observations and analyzed for fluttering amplitude and period in a water channel and in field experiments with a bottom trawl. The amplitude ratio of the fluttering ropes or nets in the tank test increased with the fluttering index as the diameter of the twine, mesh size, flexibility, and flow velocity changed, whereas the period decreased with the above factors. In bottom trawl experiments, the range of mean depth difference in the fluttering net panel was 12-17% of the length of the fluttering net, and the period of depth difference or three-dimensional (3D) tilt was revealed, with shorter ones ranging from 2 to 6 s. The amplitude as depth difference and period from field measurements were similar to those of nets in tank experiments and also to the period of 3D flow velocity inside the cod end. These results could be used to design an ASD that could be used for to the cod end of actual towed fishing gear to reduce juvenile by-catch.
Two New Records of Juvenile Oedalechilus labiosus and Ellochelon vaigiensis (Mugiliformes: Mugilidae) from Jeju Island, Korea, as Revealed by Molecular Analysis
Kwun, Hyuck Joon ; Song, Young Sun ; Myoung, Se Hun ; Kim, Jin-Koo ;
Fisheries and aquatic sciences, volume 16, issue 2, 2013, Pages 109~116
DOI : 10.5657/FAS.2013.0109
Eighteen specimens of juvenile Mugilidae were collected in October 2012 from the southern coastal waters of Jeju Island, and identified based on analysis of their mitochondrial DNA16S rRNA sequences. Seventeen specimens of Oedalechilus labiosus and a single specimen of Ellochelon vaigiensis were found, constituting a new record for these species among Korean ichthyofauna. O. labiosus is identified by the angle at the posterior end of its mouth, which contains a round notch, a darkish dorsal margin of the pectoral fin, the presence of 33-36 lateral line scales, and 23-24 vertebrae. E. vaigiensis is identified by dark dorsal and pectoral fins, the presence of 26 lateral line scales, and 25 vertebrae. The proposed Korean name for Oedalechilus is 'Sol-ip-sung-eo-sok' and that for Ellochelon is 'Nup-jeok-ggo-ri-sung-eo-sok'. The proposed Korean names for the species are 'Sol-ip-sung-eo' and 'Nup-jeok-ggo-ri-sung-eo' for O. labiosus and E. vaigiensis, respectively. We present a key for identification of the Mugilidae family of species from Korea, and include these two newly recorded species.
Wind-induced Spatial and Temporal Variations in the Thermohaline Front in the Jeju Strait, Korea
Han, In-Seong ; Suh, Young-Sang ; Seong, Ki-Tack ;
Fisheries and aquatic sciences, volume 16, issue 2, 2013, Pages 117~124
DOI : 10.5657/FAS.2013.0117
We investigated the short-term and local changes in the thermohaline front in the Jeju Strait, Korea, which is usually formed during winter and spring. To do so, we compared Real-Time Observation System by Ferryboat (RTOSF) data with wind data and routinely collected oceanographic data. During February and April 2007, a thermohaline front formed in the Jeju Strait around the 13-
isotherms and 33.0-33.5 isohalines. The thermohaline was clearly weakened and began moving southward in mid-March. The variations in the surface temperature and salinity showed a continuous north-south oscillation of the thermohaline front with a period of 3-10 days. The speed of the short-term and local fluctuation of thermohaline front was about 5-30 cm/s. We confirmed these findings by examining the variation in the maximum temperature gradient and
isotherm during the study period. These short-term and local changes had not been previously detected using serial oceanographic and satellite data. Analysis of local wind data revealed a northerly wind fluctuation with a period of 3-10 days, which was clearly related to the short-term and local changes in the thermohaline front. The short-term and local changes of the thermohaline front in the Jeju Strait originated from local changes in the winter monsoon in this area.
Phenotypic Diversity of Vibrio ichthyoenteri Isolated from the Gastrointestinal Tract of Larval Olive Flounder Paralichthys olivaceus
Han, Hyun-Ja ; Lee, Deok-Chan ; Kim, Do-Hyung ; Choi, Hye Sung ; Jung, Sung Hee ; Kim, Jin Woo ;
Fisheries and aquatic sciences, volume 16, issue 2, 2013, Pages 125~129
DOI : 10.5657/FAS.2013.0125
Vibrio ichthyoenteri is a facultatively anaerobic gram-negative bacterium with straight or slightly curved rod morphology. The bacterium is an etiological agent of bacterial enteritis of Olive flounder Paralichthys olivaceus. Only a handful of studies, using a limited number of isolates, have investigated the phenotypic and genetic characteristics of V. ichthyoenteri. We isolated 40 V. ichthyoenteri strains, identified based on the 16S rRNA gene sequence, from the diseased flounder larvae and investigated the API 20E and ZYM profiles. The isolates exhibited highly divergent phenotypic characteristics regardless of sampling time point and location, and fish age. Essential enzymes produced by V. ichthyoenteri seemed to be alkaline phosphatase, leucine arylamidase, and N-acetyl-
-glucosaminidase. This study reveals a much greater enzymatic and biochemical phenotype diversity than has been evident to date. These results suggest that a given population of V. ichthyoenteri could be heterogeneous in terms of its phenotypic characteristics.
Detection of Ostreid Herpesvirus 1 from adult Pacific Oysters Crassostrea gigas Cultured in Korea
Jee, Bo Young ; Lee, Su Jin ; Cho, Mi Young ; Lee, Soon Jeong ; Kim, Jin Woo ; Choi, Seung Hyuk ; Jeong, Hyun Do ; Kim, Ki Hong ;
Fisheries and aquatic sciences, volume 16, issue 2, 2013, Pages 131~135
DOI : 10.5657/FAS.2013.0131
The presence of ostreid herpesvirus 1 (OsHV-1) and the percentage of viral DNA detected in Pacific oyster Crassostrea gigas adults were investigated monthly between May and November 2012 at three locations along the southern coast of Korea. Among 210 oysters examined by polymerase chain reaction (PCR) analysis, OsHV-1 DNA was detected in only one oyster collected in August. The low detection rate of OsHV-1 DNA was consistent with the lack of reported OsHV-1-associated disease in C. gigas cultured in Korea. The sequence of the present PCR product amplified with the C2/C6 primer pair was identical to that of OsHV-1
except for one nucleotide, and the sequence amplified with Del36-37F2/Del36-37R showed a 605-bp deletion as in OsHV-1
. Although these sequence data are insufficient to determine genotype, the results suggest that the herpesvirus detected was similar to OsHV-1
. This is the first report on the presence of OsHV-1 in adult Pacific oysters cultured in Korea.