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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Fisheries and aquatic sciences
Journal Basic Information
Journal DOI :
The Korean Society of Fisheries and Aquatic Science
Editor in Chief :
Sungchul C. Bai
Volume & Issues
Volume 16, Issue 4 - Dec 2013
Volume 16, Issue 3 - Sep 2013
Volume 16, Issue 2 - Jun 2013
Volume 16, Issue 1 - Mar 2013
Selecting the target year
Variation and Profile of Paralytic Shellfish Poisoning Toxins in Jinhae Bay, Korea
Mok, Jong-Soo ; Song, Ki-Cheol ; Lee, Ka-Jeong ; Kim, Ji-Hoe ;
Fisheries and aquatic sciences, volume 16, issue 3, 2013, Pages 137~142
DOI : 10.5657/FAS.2013.0137
To understand critical aspects of paralytic shellfish poisoning (PSP) in a chief area of bivalve production in Korea, seasonal variation in PSP toxins in bivalves collected from Jinhae Bay, Korea in 2009 was surveyed by the pre-column high-performance liquid chromatography oxidation method. We also confirmed the profiles of major bivalves such as oysters Crassostrea gigas and mussels Mytilus galloprovincialis in Jinhae Bay. PSP toxins in the bivalves showed remarkable seasonal variation. PSP toxin levels were detected from April to May in 2009, and the highest total toxin levels at all stations were recorded in May. The major toxins in bivalves were gonyautoxin [GTX] 1&4 and C 1&2; in oysters GTX 2&3 were also detected as major components. GTX 1&4, which showed the highest PSP toxin levels at each station, accounted for the highest proportions of toxin components in mussels and oysters (64.5-71.3% and 41.4-42.4%, respectively). It was also confirmed that the highest toxicity (in
saxitoxin [STX] eq/g) was derived from GTX 1&4. The highest total toxicity (in
STX eq/g) was approximately 2-8-fold higher in mussels than in oysters collected from the same station. PSP toxin levels in bivalves differed significantly according to the sample collection station. However, the profiles of toxins in the bivalves did not show significant differences during the survey period according to sample collection station. This study shows that PSP toxin levels in some samples from Jinhae Bay were above the regulatory limit in Korea during a specific period in spring.
Effects of Carrageenan on the Gelatinization of Salt-Based Surimi Gels
Eom, Sung-Hwan ; Kim, Jung-Ae ; Son, Byoung-Yil ; You, Dong Hyun ; Han, Jeong Min ; Oh, Jung-Hwan ; Kim, Bong-Yeun ; Kong, Chang-Suk ;
Fisheries and aquatic sciences, volume 16, issue 3, 2013, Pages 143~147
DOI : 10.5657/FAS.2013.0143
The influence of carrageenan addition on the gelatinization of salt-based surimi gels was investigated by measuring compressive properties and Hunter color scale values. Hydrocolloid kappa (
)-carrageenan at 0%, 0.2%, and 1.0% and NaCl (2% w/w), KCl (1.5% w/w), and a mixture of NaCl (2% w/w) and KCl (1.5% w/w), were added to Alaska pollock surimi. Gel compressive properties were assessed by measuring the breaking force and gel strength. The gelling property of
-carrageenan-induced surimi gel was significantly increased by the incorporation of KCl rather than NaCl. The addition of
-carrageenan increased the breaking force and gel strength of surimi gels. Gels with 1%
-carrageenan and KCl had the highest breaking force and gel strength. The addition of
-carrageenan caused an increase in the whiteness values of the surimi gels.
The Growth, Innate Immunity and Protection against H
-Induced Oxidative Damage of a Chitosan-Coated Diet in the Olive Flounder Paralichthys olivaceus
Samarakoon, Kalpa W. ; Cha, Seon-Heui ; Lee, Ji-Hyeok ; Jeon, You-Jin ;
Fisheries and aquatic sciences, volume 16, issue 3, 2013, Pages 149~158
DOI : 10.5657/FAS.2013.0149
We demonstrate enhanced growth, innate immunity and protection against hydrogen peroxide (
)-induced protein oxidation and cellular DNA damage in olive flounder Paralichthys olivaceus fed a chitosan-coated moist pallet (MP) diet. A chitosan-based biopolymer coated MP as the experimental diet and a non-coated MP (control) was fed to olive flounder fish. Growth, including the average weight gain (g/fish), weight gain (%) and feed intake (g) of the fish group fed a chitosan-coated MP diet increased significantly. The survival rate was reported as 100% throughout the experimental period. Immunological parameters indicated higher mucus lysozyme activity and significantly higher fish skin mucus total protein content was observed in fish fed the chitosan-coated MP diet compared to the control. A blood plasma analysis revealed attenuation of cellular DNA and protein oxidative damage caused by
-induced oxidative stress in the fish fed the chitosan-coated MP diet compared to the control group. Moreover, blood serum biochemical analysis revealed health-promoting effects, including significantly higher hemoglobin and total cholesterol levels in the fish fed the chitosan-coated MP diet compared to the control group. In conclusion, growth, innate immunity and protection against oxidative stresses were improved by feeding of the chitosan-coated MP diet to olive flounder reared in aquaculture.
Comparison of MBA and HPLC Post-column Oxidation Methods for the Quantification of Paralytic Shellfish Poisoning Toxins
Yu, Hongsik ; Lim, Keun Sik ; Song, Ki Cheol ; Lee, Ka Jeong ; Lee, Mi Ae ; Kim, Ji Hoe ;
Fisheries and aquatic sciences, volume 16, issue 3, 2013, Pages 159~164
DOI : 10.5657/FAS.2013.0159
The mouse bioassay and high performance liquid chromatography (HPLC) post-column oxidation method are different methods of quantifying paralytic shellfish poisoning toxins. In this study, we compared their ability to accurately quantify the toxicity levels in two types of field sample (oysters and mussels) with different toxin profiles for routine regulatory monitoring. A total of 72 samples were analyzed by both methods, 44 of which gave negative results, with readings under the limit of detection of the mouse bioassay (
saxitoxin [STX] eq). In 14 oysters, the major toxin components were gonyautoxin (GTX) 1, -2, -3, -4, -5, decarbamoylgonyautoxin-2 (dcGTX2), and decarbamoylsaxitoxin (dcSTX), while 14 mussels tested positive for dcSTX, GTX2, -3, -4, -5, dcGTX2, neosaxitoxin (NEO), STX, and dcSTX. When the results obtained by both methods were compared in two matrices, a better correlation (
) was obtained for mussels than for oysters (
). Additional studies are therefore needed in oysters to investigate the differences in the results obtained by both methods. Importantly, some samples with toxin levels around the legal limit gave inconsistent results using HPLC-based techniques, which could have a strong economic impact due to enforced harvest area closure. It should therefore be determined if all paralytic shellfish poisoning toxins can be quantified accurately by HPLC, and if the uncertainties of the method lead to doubts regarding regulatory limits.
Molecular and Histopathological Evidence of Mycobacteriosis in Paradise Fish Macropodus opercularis Imported into Korea
Han, Hyun-Ja ; Kim, Jeong-Ho ; Jeon, Chan-Hyeok ; Kim, Wi-Sik ; Kim, Do-Hyung ; Jung, Sung-Ju ; Oh, Myung-Joo ;
Fisheries and aquatic sciences, volume 16, issue 3, 2013, Pages 165~169
DOI : 10.5657/FAS.2013.0165
We report on mycobacteriosis in an imported tropical ornamental fish Macropodus opercularis commonly known as the paradise fish. Mass mortality occurred in paradise fish imported to Korea from Southeast Asia in 2008. The affected fish did not show any outward clinical signs, but enlargement of the spleen, kidneys, and liver was observed on dissection. Histopathological examination revealed numerous granulomas in the spleen, and acid-fast bacilli were observed in the centers of the granulomas. About 65% of spleen DNA samples were PCR positive using mycobacteria-specific primers targeting the 16S rRNA and hsp65 genes. The nucleotide identities of the 16S rRNA and hsp65 genes with those of Mycobacterium marinum were 99.5% and 99.4%, respectively. Although the bacterium was not cultured, the molecular diagnosis and histopathological findings were consistent with mycobacteriosis in paradise fish.
Toxic Effects on the Nonspecific Immune System of the Rock Bream Oplegnathus fasciatus upon Exposure to Di-2-ethylhexyl Phthalate
Kim, Jun-Hwan ; Jeong, Dal-Sang ; Kang, Ju-Chan ;
Fisheries and aquatic sciences, volume 16, issue 3, 2013, Pages 171~176
DOI : 10.5657/FAS.2013.0171
The aim of this study was to investigate the in vivo toxicity of di-2-ethylhexyl phthalate (DEHP), on the immune system of the rock bream, Oplegnathus fasciatus. Fish were injected twice intraperitoneally with DEHP (200, 400, and 800 mg/kg BW), and the cellularity and functional activity of phagocytes in the spleen and head kidney were measured. The number of head kidney leukocyte cells was significantly greater in fish treated with 800-mg DEHP/kg BW. Nonspecific immunity, as determined by the phagocytic index, was significantly decreased at 800-mg DEHP/kg BW in the head kidney. A significant reduction in phagocytic capacity was observed in the head kidney at
400-mg DEHP/kg BW. Furthermore, significantly increased levels of serum glutamic oxaloacetate transaminase and glutamic pyruvate transaminase indicated a marked hepatic dysfunction in immunosuppressed fish. Total serum protein was significantly reduced at
400-mg DEHP/kg BW; however, there were no significant changes in lysozyme activity. These results demonstrate that immune responses in the rock bream, Oplegnathus fasciatus can predict immunotoxicity at doses
400-mg DEHP/kg BW.
Establishment and Characterization of Permanent Cell Lines from Oryzias dancena Embryos
Lee, Dongwook ; Kim, Min Sung ; Nam, Yoon Kwon ; Kim, Dong Soo ; Gong, Seung Pyo ;
Fisheries and aquatic sciences, volume 16, issue 3, 2013, Pages 177~185
DOI : 10.5657/FAS.2013.0177
The development of species-specific fish cell lines has become a valuable tool for biological research. In recent years, marine medaka Oryzias dancena has been recognized as a good experimental model fish but there are no reports of establishment of cell lines from this fish. In this study, two cell lines from O. dancena blastula embryos were established from 41 total trials (4.9%). The two cell lines displayed typical in vitro morphology and have been cultured for >121 passages, which corresponds to 293 days. The doubling times of the cell lines were 29.84 and 28.59 h, respectively, and both possessed the potential to expand in a clonal manner, albeit with significant differences between the two cell lines. The absence of any of the four main medium supplements; i.e., fish serum, fetal bovine serum, basic fibroblast growth factor, and medaka embryo extract, significantly inhibited growth. The proportion of cells possessing normal chromosome number was 45% and 46.7% of the cell lines, respectively. Taken together, two cell lines that proliferate continuously were established from marine medaka and these cell lines may provide a basic tool for characterizing the unique features of this fish species.
Quantification of the Sub-lethal Toxicity of Metals and Endocrine-disrupting Chemicals to the Marine Green Microalga Tetraselmis suecica
Ebenezer, Vinitha ; Ki, Jang-Seu ;
Fisheries and aquatic sciences, volume 16, issue 3, 2013, Pages 187~194
DOI : 10.5657/FAS.2013.0187
Microalgae are sensitive indicators of environmental changes, and hence they are widely used in environmental risk assessments and for the development of discharge guidelines. Here we evaluated the toxicity of metals and endocrine-disrupting chemicals (EDCs) to the marine green microalga, Tetraselmis suecica. The toxicants investigated included the metals, Cu, Ni, and Pb; and the EDCs, bisphenol A (BPA), endosulfan (ES), and polychlorinated biphenyl (PCB). The endpoints were variations in cell counts and chlorophyll a levels. T. suecica displayed a varied pattern of sensitivity to the toxicants. Based on the 72-h median effective concentration (
), ES (0.045 mg/L) was most toxic to T. suecica, followed by PCB (3.96 mg/L) and Pb (9.62 mg/L). Interestingly, T. suecica was relatively tolerant to Cu (43.03 mg/L). The 72-h
values of Ni and BPA were approximately 16 mg/L. Our data suggest that this species may be relatively tolerant to most of the chemicals within their permissible limits in the environment.
First Record of the Stichaeid Fish Chirolophis saitone (Perciformes: Stichaeidae) from Korea
Kim, Maeng Jin ; Choi, Jung Hwa ; Lee, Dong Woo ; Kim, Jung Yun ; Choi, Kwang Ho ;
Fisheries and aquatic sciences, volume 16, issue 3, 2013, Pages 195~197
DOI : 10.5657/FAS.2013.0195
One specimen of Chirolophis saitone, belonging to the family Stichaeidae, was collected by a bottom trawl in the southern area of the Yellow Sea. The present specimen was characterized by 52 dorsal fin spines, 38 anal fin spines, a caudal fin with four vertical dark bands and an orbital region with three pairs of branched cirri. We propose a new Korean name, "Jak-eun-goe-do-ra-chi', for C. saitone.
New Records of Three Sergestid Shrimps (Crustacea: Decapoda: Penaeidea) from the Korean Waters of the Yellow Sea
Kim, Jung Nyun ; Choi, Jung Hwa ; Lee, Jeong-Hoon ; Kim, Joo Il ;
Fisheries and aquatic sciences, volume 16, issue 3, 2013, Pages 199~205
DOI : 10.5657/FAS.2013.0199
Three species of pelagic sergestid shrimps, Deosergestes seminudus (Hansen, 1919), Sergia lucens (Hansen, 1922) and S. talismani (Barnard, 1947), were identified based on collections by the National Fisheries Research and Development Institute from the Korean coast of the Yellow Sea between 2003 and 2011. They are herein reported for the first time from the Yellow Sea. Morphological descriptions and illustrations with color photographs of all species are also given. With the addition of these species, the family Sergestidae in the Korean waters now comprises five species belonging to three genera. Finally, a key to the Korean genus and species of the family Sergestidae is presented.
First Record of the Eyeshade Sculpin Nautichthys pribilovius (Hemitripteridae: Pisces) from the East Sea, Korea
Lee, Soo Jeong ; Jung, Yeon-Soo ; Kweon, Seon-Man ; Kim, Jin-Koo ;
Fisheries and aquatic sciences, volume 16, issue 3, 2013, Pages 207~209
DOI : 10.5657/FAS.2013.0207
A new record of the eyeshade sculpin Nautichthys pribilovius is described on the basis of a single specimen collected from the East Sea, Korea. The species has 23 second dorsal fin rays, rounded spines on the occiput, a black band through the eye and across the cheek, branched caudal fin rays and a branchiostegal membrane attached to the isthmus. The new Korean name "Gum-eun-tti-nun-hoet-dae-sok" is proposed for the genus Nautichthys, and "Gum-eun-tti-nun-hoet-dae" is proposed for the species N. pribilovius.
Shaking Motion Characteristics of a Cod-end Caused by an Attached Circular Canvas during Tank Experiments and Sea Trials
Kim, Yonghae ;
Fisheries and aquatic sciences, volume 16, issue 3, 2013, Pages 211~220
DOI : 10.5657/FAS.2013.0211
A shaking motion could be used to improve fish escapement from a cod-end net by creating a sieving effect over the swept volume or by disturbing the optomotor response of the fish. In this study, a perpendicular shaking motion was generated in a towed cod-end net by a circular canvas attached to the end of the codend, which formed a biased cap-like shape. This concept was tested by using a model in a flow tank and by towing a prototype cod-end during sea trials. For the model tests, the amplitude of the shaking motion was
times the rear diameter of the cod-end, and the period of the shaking motion was
s at a flow velocity of 0.6 or 0.8 m/s. In the sea trials, the amplitude was
times the rear diameter of the cod-end, and the period of the shaking motion was
s at towing speeds of 1.2 or 1.7 m/s. Thus, the shaking amplitude during the sea trials was equal to or less than that observed in the tank tests, and the shaking period was twice as long. The shaking motion described by the amplitude and period could be an effective means to stimulate fish escapement from cod-end during fishing operations considering the response of the fish.