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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Fisheries and aquatic sciences
Journal Basic Information
Journal DOI :
The Korean Society of Fisheries and Aquatic Science
Editor in Chief :
Sungchul C. Bai
Volume & Issues
Volume 18, Issue 4 - Dec 2015
Volume 18, Issue 3 - Sep 2015
Volume 18, Issue 2 - Jun 2015
Volume 18, Issue 1 - Mar 2015
Selecting the target year
Negative Correlation between the Prevalence of Norovirus and High Bacterial Loads of Escherichia coli in Oysters Crassostrea gigas
Oh, Eun-Gyoung ; Song, Ki Cheol ; Kim, Sukyung ; Park, Kunbawui ; Yu, Hongsik ;
Fisheries and aquatic sciences, volume 18, issue 3, 2015, Pages 235~240
DOI : 10.5657/FAS.2015.0235
Seasonal variation in the prevalence of norovirus in oysters Crassostrea gigas was investigated and compared to levels of Escherichia coli, a fecal indicator in oysters. Oysters were collected from Iwon-myeon, Taean-gun, Korea, a primary production area for European Union export of oysters between 2013 and 2014. We observed seasonality in the prevalence of norovirus in oysters, with a higher prevalence and viral load detected during winter months. Oysters taken from production areas that complied with the European Union standard for raw consumption (< 230 MPN/100 g of E. coli) had 22.1% of the samples test positive for norovirus (15/68 samples). However, norovirus was not detected in any of the samples (0%, 0/4 samples) that were collected from production sites that exceeded the standard fecal contamination level for raw consumption (> 230 MPN/100 g of E. coli). These results indicated that there is a negative correlation between the prevalence of norovirus and high levels of E. coli in oysters. Therefore, our results suggest that current food safety guidelines using only a bacterial fecal contamination indicator, E. coli, may not adequately assess shellfish production areas for viral and bacterial contamination.
In vitro Antibacterial and Synergistic Activity of an Ecklonia cava Extract against Anti biotic-Resistant Streptococcus parauberis
Eom, Sung-Hwan ; Santos, Jeniel A. ; Kim, Ji-Hoon ; Jung, Won-Kyo ; Kim, Do-Hyung ; Kim, Young-Mog ;
Fisheries and aquatic sciences, volume 18, issue 3, 2015, Pages 241~247
DOI : 10.5657/FAS.2015.0241
In an effort to discover alternative phytotherapeutic antimicrobial agents to combat Streptococcus parauberis, a fish pathogenic bacterium, we evaluated the antibacterial activity of seaweed extracts in vitro. A methanolic extract of Ecklonia cava exhibited strong antibacterial activity against S. parauberis isolated from olive flounder Paralichthys olivaceus. Furthermore, the n-hexane soluble (Hexane) fraction of the E. cava methanolic extract exhibited the greatest antibacterial effect on S. parauberis strains with a minimum inhibitory concentration (MIC) ranging from 256 to
. In addition, the MIC values of oxytetracycline against antibiotic-resistant S. parauberis were markedly reduced up to 64-fold in combination with the Hexane fraction, suggesting that the antibacterial activity of the antibiotic was restored when combined with the Hexane fraction. The interaction between both antibiotics and the Hexane fraction was assessed by the fractional inhibitory concentration (FIC) index. The Hexane fraction and oxytetracycline combination against antibiotic-resistant S. parauberis strains resulted in a median
range of 0.502 to 0.516. Thus, the synergistic ranges of median
< 1 were observed for all combinations of the Hexane fraction and oxytetracycline against S. parauberis. To the best of our knowledge, this is the first report indicating the efficacy of an E. cava extract against fish pathogenic bacterium S. parauberis.
Dietary Sulfur Amino Acids Can Spare Taurine in Rock Bream Oplegnathus fasciatus
Ferreira, Fernando Magalhaes ; Yun, Hyeonho ; Park, Youngjin ; Lee, Seunghan ; Park, Gunhyun ; Bai, Sungchul C. ;
Fisheries and aquatic sciences, volume 18, issue 3, 2015, Pages 249~255
DOI : 10.5657/FAS.2015.0249
An 8 week feeding trial was conducted to evaluate whether methionine and cysteine would effectively spare taurine supplementation on growth performance in juvenile rock bream Oplegnathus fasciatus. Triplicate groups of 25 fish averaging
) were fed one of the experimental diets. Five experimental diets including a fish meal based control diet were prepared by adding sulfur amino acid at fixed level of 1.0% and taurine at graded levels of 0%, 0.25%, 0.5% and 1.0% (
, respectively). After the feeding trial, growth performance of fish fed the
diets were significantly higher (P < 0.05) than those of fish fed the Control diet. ANOVA test suggested that when sulfur amino acid were supplemented to the diets, the optimum taurine supplementation level could be 0.25% in the diet, and broken line analysis of weight gain indicated a level of 0.33%, for positive effects on growth and feed utilization. Fish whole-body protein content and taurine concentration steadily increased with the increase of dietary level in the presence of sulfur amino acid in the diets. On the other hand, whole-body lipid content significantly decreased with the incremental levels of dietary taurine. In conclusion, the results of the present study clearly indicated that dietary supplementation of methionine and cysteine at a level of 1% could spare 0.25 to 0.33% of taurine in juvenile O. fasciatus diets.
Evaluation of Coffee Ground as a Feedstuff in Practical Diets for Olive Flounder Paralichthys olivaceus
Rahimnejad, Samad ; Choi, Jin ; Lee, Sang-Min ;
Fisheries and aquatic sciences, volume 18, issue 3, 2015, Pages 257~264
DOI : 10.5657/FAS.2015.0257
A 10-week feeding trial was carried out to investigate the feasibility of using spent coffee ground (CG) as a potential feed ingredient for olive flounder. Growth, feed utilization, body composition and antioxidant enzyme activity were examined. A control diet was formulated and three other diets were prepared to contain 5, 10 or 15% CG (designated as Con, CG5, CG10 and CG15, respectively) by replacing for wheat flour. Two hundred forty fish (
) were allotted to 12 circular tanks of 400 L capacity at a density of 20 fish per tank and fed the experimental diets twice daily. At the end of the feeding trial, fish fed the CG5 diet exhibited significantly (P < 0.05) higher growth performance than those fed the control diet. Also, fish fed the CG10 diet had a comparable growth to that of the control group, but further increase of dietary CG inclusion level to 15% resulted in significant decrease of growth performance. Fish fed the CG15 diet showed significantly lower feed efficiency and protein efficiency ratio than other treatments. Significantly lower muscle protein content was observed in fish fed CG15 diet compared to the control. Significant reduction in plasma cholesterol concentration was found in fish fed CG15 diet compared to control. No significant changes were found in alkyle and superoxide radicals scavenging activities of plasma, muscle and liver among dietary treatments. Also, liver total protein, total antioxidant capacity, catalase and glutathione peroxidase activities were not significantly influenced by dietary inclusion of CG. According to these results, we concluded that CG can be included up to 10% in the diet for olive flounder without any adverse effects on growth, feed utilization and antioxidant enzyme activity.
Optimal Dietary Protein and Lipid Levels for Growth of Juvenile Israeli Carp Cyprinus carpio
Aminikhoei, Zahra ; Choi, Jin ; Lee, Sang-Min ;
Fisheries and aquatic sciences, volume 18, issue 3, 2015, Pages 265~271
DOI : 10.5657/FAS.2015.0265
A feeding trial of four dietary protein levels (20, 30, 40, and 50%) and two lipid levels (7 and 14%) with a factorial design was conducted to determine the optimal dietary protein and lipid levels for juvenile Israeli carp Cyprinus carpio. Triplicate groups of fish (average body weight,
) were fed the experimental diets for 9 weeks. Survival of fish was not affected by either dietary protein or dietary lipid level. Weight gain and feed efficiency increased as dietary protein levels increased up to 40 and 50%, respectively. Weight gain was higher in fish fed the high-lipid diets with 20 and 40% protein content. Feeding efficiency increased as the dietary lipid level increased for the 30, 40, and 50% protein diets. Daily feed intake decreased with increasing protein level and the minimum feed consumption was observed in fish fed the 50% protein diet with 14% lipid content. Moisture and lipid contents of the whole body were affected by both dietary protein and lipid levels. The crude lipid content of fish fed the 14% lipid diet was higher than that of fish fed the 7% lipid diet at each protein level. The results of this study indicate that a diet containing 40% protein with 14% lipid content is optimal for the growth and effective protein utilization of juvenile Israeli carp.
The Molecular Profiling of a Teleostan Counterpart of Follistatin, Identified from Rock Bream Oplegnathus fasciatus which Reveals its Transcriptional Responses against Pathogenic Stress
Herath, H.M.L.P.B ; Priyathilaka, Thanthrige Thiunuwan ; Elvitigala, Don Anushka Sandaruwan ; Umasuthan, Navaneethaiyer ; Lee, Jehee ;
Fisheries and aquatic sciences, volume 18, issue 3, 2015, Pages 273~281
DOI : 10.5657/FAS.2015.0273
The follistatin (FST) gene encodes a monomeric glycoprotein that plays a role in binding and inhibiting the functions of members of the transforming growth factor (TGF)-
superfamily. Thus, FST facilitates a wide variety of functions, ranging from muscle growth, to inflammation and immunity. In this study, we sought to characterize an FST counterpart, RbFST, which was identified from rock bream Oplegnathus fasciatus. The RbFST cDNA sequence (2,419 bp) contains a 933-bp open reading frame (ORF) that encodes a putative amino acid sequence for RbFST (35 kDa). The putative amino acid sequence contains a Kazal-type serine protease inhibitor domain (51-98 residues) and an EF-hand, calcium-binding domain (191-226 residues). Additionally, this sequence shares a high identity (98.7%) with the Siniperca chuatsi FST sequence, with which it also has the closest evolutionary relationship according to a phylogenetic study. Omnipresent distribution of RbFST transcripts were detected in the gill, liver, spleen, head kidney, kidney, skin, muscle, heart, brain, and intestine of healthy animals, with significantly higher expression levels in the heart, followed by the liver tissue. Under pathogenic stress caused by two bacterial pathogens, Streptococcus iniae and Edwardsiella tarda, RbFST transcription was found to be significantly up-regulated. Altogether, our findings suggest the putative role of RbFST in immune related responses against pathogenic infections, further prefiguring its significance in rock bream physiology.
First Record of the Antrorse Spined Gurnard Pterygotrigla multiocellata (Triglidae, Scorpaeniformes) from Korea
Kim, Joon Sang ; Song, Choon Bok ;
Fisheries and aquatic sciences, volume 18, issue 3, 2015, Pages 283~286
DOI : 10.5657/FAS.2015.0283
This is the first report of Pterygotrigla multiocellata from the family Triglidae, collected in Korea. The specimen (314 mm in standard length) was caught in a gill net in the coastal waters of Jeju Island on 11 February 2010. This species is characterized by the following morphological traits: head large and triangular, bony plates absent along the base of the second dorsal fin, antrorse rostral spine long, and cleithral spine long. We suggest a new Korean name, "Gin-ppul-mit-seong-dae" for P. multiocellata.
No Response to Bidirectional Size-Based Selection in the Rotifer Brachionus rotundiformis
Malekzadeh-Viayeh, Reza ; Song, Choon Bok ;
Fisheries and aquatic sciences, volume 18, issue 3, 2015, Pages 287~296
DOI : 10.5657/FAS.2015.0287
Although rotifers have been considered the best feeding option for several species of fishes in aquaculture, they are sometimes larger than appropriate for the early larval stage of some marine fishes. Thus, we aimed to determine whether size-based selection of the parents could affect the average body size of their progeny in two clonal populations of the rotifer Brachionus rotundiformis. From each of the clones, 20 individuals were bi-directionally selected toward both smaller and larger sizes and each individual-based selection was conducted for 10 consecutive generations. The results showed that although there were sometimes differences in mean body size between parents and their progeny, no directional trend was observed in all selected lines of both clones. We demonstrated that artificial selection in a rotifer stock cannot lead to an expected size range although they appear to exhibit a large degree of body size polymorphism.
First Record of a Gaper Champsodon longipinnis (Perciformes: Champsodontidae) from Korea
Han, Song-hun ; Kim, Maeng Jin ; Song, Choon Bok ;
Fisheries and aquatic sciences, volume 18, issue 3, 2015, Pages 297~300
DOI : 10.5657/FAS.2015.0297
This is the first report of Champsodon longipinnis (Champsodontidae) collected from Korea. The specimen (100.9 mm in standard length) was caught by a bottom trawl in the coastal waters off Busan, Korea. This species is characterized by the following morphological traits: body with small denticulate scales except abdomen around pelvic fin base; posterior end of maxilla extending beyond margin of eye; dark pigmentation on most of the first dorsal fin, upper half of the first to the fourth soft ray of the second dorsal fin, and partial upper lobe of caudal fin. We add C. longipinnis to the Korean fish fauna and suggest a new Korean name, "Jeom-ak-eo-chi," for the species.
Ontogenetic Development of the Digestive System in Chub Mackerel Scomber japonicus Larvae and Juveniles
Park, Su-Jin ; Lee, So-Gwang ; Gwak, Woo-Seok ;
Fisheries and aquatic sciences, volume 18, issue 3, 2015, Pages 301~309
DOI : 10.5657/FAS.2015.0301
Chub mackerel, Scomber japonicus, larvae and juveniles were reared from hatching to 35 days after hatching (DAH), and the development of their digestive systems was histologically investigated. The larvae were initially fed on rotifers and Artemia nauplii starting around 19 DAH, and thereafter on Artemia nauplii, fish eggs, and a formulated feed mixture. The primitive digestive system differentiated at 3 DAH; the digestive tract was distinctively divided into the buccopharyngeal cavity, esophagus, stomach, air bladder, intestines, and rectum. The gastric gland and pyloric caeca first appeared at 5 and 7 DAH, respectively. The stomach was divided into cardiac, fundic, and pyloric regions in the preflexion phase. The number of gastric glands and pyloric caeca, as well as the volume of the gastric blind sac increased markedly, with development continuing into the juvenile stage. The precocious development of the digestive system during the larval period might be related to the early appearance of piscivory, which is able to support high growth potential. The organogenesis results obtained for this precocial species represent a useful tool to aid our understanding of the physiological requirements of larvae and juveniles to ensure optimal welfare and growth under aquaculture conditions, which will improve current rearing practices of this scombrid species.
New Record of Brama dussumieri (Pisces: Bramidae) from Korea, as Revealed by Morphological and Molecular Analyses
Lee, Woo Jun ; Kim, Jin-Koo ;
Fisheries and aquatic sciences, volume 18, issue 3, 2015, Pages 311~316
DOI : 10.5657/FAS.2015.0311
Ten specimens of Brama dussumieri (family Bramidae) were collected from waters off Jeju Island, Busan, and Gangneung, Korea, during 2013-2014. The specimens were characterized by having 58-64 lateral line scales and 13-15 gill rakers. An analysis of 567 base pair sequences of mitochondrial DNA cytochrome c oxidase subunit I showed that sequences in our ten specimens are concordant with those of B. dussumieri from the USA, India, and Japan, although with slight differences (genetic distance = 0.000-0.018). Brama dussumieri was distinguished from the most similar species, Brama japonica, by the number of lateral line scales (57-65 in B. dussumieri vs. 65-75 in B. japonica) and the number of gill rakers (13-15 in B. dussumieri vs. 17-20 in B. japonica). We propose the new Korean name "Wae-sae-da-rae" for B. dussumieri in Korea.
New Record of Nomeus gronovii (Pisces: Nomeidae) from Korea
Lee, Soo Jeong ; Kim, Jin-Koo ; Lee, Won-Chan ; Kim, Jeong Bae ; Kim, Hyung Chul ;
Fisheries and aquatic sciences, volume 18, issue 3, 2015, Pages 317~320
DOI : 10.5657/FAS.2015.0317
A new record of Nomeus gronovii is described here based on a single specimen collected off the Jeju Island, Korea. The species has a blotched and spotted pattern on the body, 41 vertebrae, and a large pectoral fin with a white lower part. A molecular analysis of the partial (500 bp) cytochrome c oxidase subunit I gene showed that this specimen is closely related to specimens of N. gronovii collected in South Africa (Kimura-two-parameter distance = 0.006-0.012). A new Korean name, "Ga-neun-dong-gang-yeon-chi", is proposed for this species.
First Zoea of Sesarmops intermedius (De Haan, 1835) (Crustacea: Decapoda: Brachyura: Sesarmidae)
Lee, Hwa Ja ;
Fisheries and aquatic sciences, volume 18, issue 3, 2015, Pages 321~324
DOI : 10.5657/FAS.2015.0321
The first zoeal characters of Sesarmops intermedius (De Haan, 1835) [=Sesarma (Sesarma) intermedium], belonging to the family Sesarmidae, are described and illustrated in detail based on laboratory-hatched material from a female collected from Gangjeung, Seogwipo, Jeju Island. Morphological comparison is performed based on previous descriptions of Sesarma (Sesarma) intermedium from Japan and three other species in the Sesarmidae. The first zoea of Sesarmops intermedius can be readily distinguished from those of the three species Chiromantes haematocheir [=Sesarma (Holometopus) haematocheir], Chiromantes dehaani [=Sesarma (Holometopus) dehaani], and Parasesarma pictum [=Sesarma (Parasesarma) pictum] by the presence of two setae on the exopod of the antenna, fine setae on the inner and outer margin of the forked spine of the telson, and three aesthetascs and one simple seta on the antennule.
Bycatch Reduction by Experimental Shaking Codend Attached with Canvas in a Bottom Trawl
Kim, Yonghae ;
Fisheries and aquatic sciences, volume 18, issue 3, 2015, Pages 325~332
DOI : 10.5657/FAS.2015.0325
An active stimulating method for juvenile fishes to drive escaping from mesh of the codend was examined by shaking canvas in the bottom trawl followed by shrimp beam trawl. Field fishing trials by a bottom trawl were carried out between the Geomoondo and Jejudo in west of South sea, Korea by conver-net methods to examine the effect on the reduction of juvenile fish as a discard catch by generating a shaking movement of the codend using two pieces of asymmetrical semi-circular canvas. The mean period of the shaking motion with the round canvas was 10-15 s, and the range of amplitude as a vertical depth change was up to 0.4-0.6 m when towing speed 3.4-4.3 k't as estimated by peak event analysis. The escape rate of juvenile fish in conver-net by total juvenile bycatch (codend and cover-net) in 14 trials increased from 20% in a steady codend to 34% using a shaking codend in the bottom trawl, while the marketing catch or total bycatch was similar between steady and shaking cod ends. There was no difference in the body size of the fish and species composition between the steady and shaking cod ends. Above results demonstrate a new method for bycatch reduction actually up to 18% using an active stimulating device, although further experiments are needed to increase an effective shaking motion of the codend in amplitude and period for more bycatch reduction.
An outbreak of Lactococcus garvieae Infection in Cage-cultured Red Lip Mullet Chelon haematocheilus with Green Liver Syndrome
Han, Hyun-Ja ; Lee, Nam Sil ; Kim, Myoung Sug ; Jung, Sung Hee ;
Fisheries and aquatic sciences, volume 18, issue 3, 2015, Pages 333~339
DOI : 10.5657/FAS.2015.0333
Red lip mullet Chelon haematocheilus (body weight =
) which became sick during an outbreak of disease at mariculture facilities at Cheonsu Bay, Korea, during July-August 2013, were examined to identify the cause of the disease. Diseased mullets displayed green liver syndrome, and Lactococcus garvieae were isolated from their internal organs. Argulus sp., Trichodina sp., and/or Vibrio spp. were also discovered in some infected fish. Histopathological examination revealed that fatty liver syndrome with hepatocyte degeneration, reflected in heterokaryons, inflammatory lesions, and melanomacrophage centers (
), had caused fibrosis around the kidney, spleen, and blood vessels. After the outbreak, visceral fat and green liver syndrome in the mullets were consistently observed throughout the year in the same mariculture facilities, indicating that the cultured mullets suffered a chronic metabolic disorder. Although Vibrio spp. were also isolated from some individuals, L. garvieae, which is known to be a causative agent of red lip mullet mortality, was isolated from all diseased individuals. This is the first report of L. garvieae infection in cultured red lip mullet.