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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Fisheries and aquatic sciences
Journal Basic Information
Journal DOI :
The Korean Society of Fisheries and Aquatic Science
Editor in Chief :
Sungchul C. Bai
Volume & Issues
Volume 2, Issue 2 - Dec 1999
Volume 2, Issue 1 - Jun 1999
Selecting the target year
Antioxidant Activity of 2,3,6-Tribromo-4,5-dihydroxy benzyl methyl ether from Symphyocladia latiuscula
Park Hye Jin ; Chung Hae Young ; Kim Jong ; Choi Jae Sue ;
Fisheries and aquatic sciences, volume 2, issue 1, 1999, Pages 1~7
Antioxidant activity of a methanol extract of Symphyocladia latiuscula was evaluated by the thiocyanate method in the linoleic acid system. The methanol extract inhibited the peroxidation of linoleic acid in a dose-dependent manner. The MeOH extract was then sequentially partitioned with n-hexane,
, EtOAc, n-BuOH and H20. The antioxidant activity of the fractions increased in order of
, n-hexane, EtOAc, and n-BuOH. There was no activity found in
partitoned fraction by the thiocyanate method. Especially, the activities of the fractions of n-hexane and
were comparable to that of 2,6-di-tertbutylhydroxytoluene (BHT). Column chromatography of the
fraction over silica gel yielded cholesterol (1) and 2,3,6-tribromo 4,5-dihydroxybenzyl methyl ether (2) which were identified by instrumental analysis of MS and
. The latter (2) demonstrated significant antioxidant activity.
Optimizing Boiling Condition for the Preparation of Fish Extracts
Park Seong Min ; Lee Keun Tai ; Yoon Ho Dong ; Ryu Hong Soo ;
Fisheries and aquatic sciences, volume 2, issue 1, 1999, Pages 8~11
The optimum boiling condition was determined for fish extracts by response surface model. Model equations were designed with effect of time (T) and the amount of added water (W) on the level of released free amino acid. Based on the high (>0.9) coefficient of determination and low (<0.01) level of significant, those model was approved to be significant. The added water amount of higher regression coefficient
, showed a greater influence on releasing free amino acids than boiling time. The optimum boiling times are 6 hours for crucian carp, 5 hours for bastard halibut, 7 hours for loach and 5 hours for jacopever. The ratio of added water to sample 1 (v/w) could be applied to all fish samples at
Freeze Denaturation of Squid Actomyosin
Lee Kang-Ho ; Ryu Hong-Soo ; Cho Young-Je ; Jung Byung-Chun ; Hong Byung-Il ; Je Yoi-Kwon ; Lee Goon-Ja ;
Fisheries and aquatic sciences, volume 2, issue 1, 1999, Pages 12~16
Denaturation of actomyosin from the obliquely striated mantle muscle of squids (Todarodes pacificus) was studied by measuring the changes in
-ATPase activity, relative viscosity, and solubility during frozen storage at three different temperature zones of maximum ice crystal formation
, the eutectic point
. The logarithms of
-ATPase activity, relative viscosity and solubility of the actomyosin solutions (0.6 M KCl) and suspensions (0.05 M KCl) tended to decrease during frozen storage. The denaturation of squid actomyosin at the zone of maximum ice crystal formation significantly differed by only two degree of temperature difference between
, and it (0.05 M KCl) at
was less than those of other temperature. The denaturation at
was more rapid than at
. The logarithms of
-ATPase activity, relative viscosity, and solubility were changed slower in the suspensions (0.05 M KCl) than the solutions (0.6 M KCl) at all experimental temperatures.
The Morphological and Growth Characteristics of Two Strains of Fibrocapsa japonica Isolated from New Zealand and Japan
Cho Eun Seob ; Rhodes Lesley L. ; Kim Hak Gyoon ;
Fisheries and aquatic sciences, volume 2, issue 1, 1999, Pages 17~24
The blooms caused by Fibrocapsa japonica have occurred regularly in New Zea1andean coastal waters, and no fish kills and economic impacts have been reported. However, Fibrocapsa japonica in Japan killed caged juvenile fish and has been regarded as a harmful micro algae. In this comparative study a New Zealand isolate was found to be morphologically similar to a Japanese isolate, although slightly larger on average than. Optimal temperatures for growth differed, with fastest growth rates occurring at
for the New Zealand strain and
for the Japanese strain, with a decrease in growth rate exhibited by the latter at
. Both isolates had low salinity optima of 20-25 PSU, although they grew between 15 and 30 PSU. Growth declined significantly for both strains above 30 PSU.
Developmental Morphology of Osmundea crispa (Ceramiales, Rhodophyta) from California
Nam Ki Wan ; Choi Han Gil ;
Fisheries and aquatic sciences, volume 2, issue 1, 1999, Pages 25~31
Developmental morphology of the red alga, Osmundea crispa (Hollenberg) Nam from California was studied on the basis of liquid-preserved and herbarium specimens. Vegetative axial segment of the species produces two pericentral cells and one trichoblast. Spermatangial filaments (branches) are derived from apical and epidermal cells in pocket-shaped apical pit with an ostiole-like upper opening. Procarp-bearing segment of female trichoblast produces five pericentral cells, of which the fifth functions as supporting cell of carpogonial branch. Tetrasporangial production occurs in random epidermal cells in apical pit of branchlets, and two presporangial cover cells show parallel arrangement to stichidial axis. As this vegetative and reproductive development is included in the generic delineation of Osmundea Stackhouse, O. crispa among the known Osmundea species is characterized by habit forming compact cushion-like clump with angular to terete thallus. It is also distinguished from O. hybrida (AP. de Candolle) Nam without the compressed thallus by the number of pericentral cells in procarp-bearing segment and shape of spermatangial pit. Taxonomic implication of the shape of spermatangial pit is also included.
Two Bucephalid Parasites, Dolichoenterum longissimum and Prosorhynchus aculeatus (Trematoda: Digenea), of Conger Eel, Conger myriaster, from Coastal Areas in Korea
Kim Ki Hong ; Kwon Se Ryun ; Huh Sung-Hoi ;
Fisheries and aquatic sciences, volume 2, issue 1, 1999, Pages 32~35
During the course of studying the helminth fauna from the fishes of the Korean coastal waters, two bucephalid species, Dolichoenterum longissimum Ozaki, 1924 and Prosorhynchus aculeatus Odhner, 1905, were collected from the alimentary canal of the conger eel, Conger myriaster. D. longissimum was characterized by having the ovary between the testes, and 7-8 horn-like projections on the ventral side of rhynchus. P. aculeatus was distinguished from the other species by the location of the mouth or the opening position of the excretory vesicle. These two bucephalid digeneans are new to the Korean fauna.
A Study on the Rapid Hydrolysis of Fish Using Proteolytic Bacteria Isolated from Anchovy Jeotkal
Kim Sang-Ho ; Kim Young-Min ; Seong Hee-Kyung ; Choi Su-Il ; Kim Seon-Bong ; Han Bong-Ho ;
Fisheries and aquatic sciences, volume 2, issue 1, 1999, Pages 36~43
A study on the hydrolysis of anchovy using proteolytic bacteria isolated from anchovy jeotkal (a salt-fermented fish) was carried out to develop a rapid process of liquefied anchovy jeotkal. Five kinds of proteolytic bacteria, such as Staphylococcus sp.-l, Photobacterium sp., Volcaniella sp., Staphylococcus sp.-2 and Bacillus sp., were isolated from the anchovy jeotka1 that fermented with
NaCl at room temperature for 2 months. Those grew well at
, pH 7.0 on TPY broth with
NaCl. The optimal hydrolysis temperature, pH, time and proteolytic bacteria densities for hydrolysis of minced anchovy were
, 7.0, 6 hours and
cells/g raw anchovy, respectively.
The Growth of Proteolytic Bacteria Immobilized in Capsule Type
Han Bong-Ho ; Choi Su-Il ; Kim Seong-Bong ; Kim Sang-Ho ;
Fisheries and aquatic sciences, volume 2, issue 1, 1999, Pages 44~51
Proteolytic bacteria isolated from fermented anchovy jeotkal were immobilized in capsule type with
sodium alginate and
cellulose (CMC). For making the immobilized capsule, the optimal concentration of both
and CMC, with respect to the membrane hardness and the growth of proteolytic bacteria in capsule, were
at following conditions: flow rate of
solution and cell suspension were respectively 3.54 ml/min and 0.15 ml/min when inside diameter of inner and outer capillary tube in immobilizing apparatus were 0.32mm, 0.74mm, respectively. The density of proteolytic bacteria in capsule reached maximum, i.e.
/capsule during culture under optimal conditions in TPY broth, and these were
times higher than these of before culture. During culture of proteolytic bacteria immobilized in capsule type (PBImC) for 72hrs, few growing cells were lost in the outer medium.
Performance of Rotating Biological Contactor (RBC) under Different Hydraulic Loading Rates and Rotational Speeds on Ammonia Removal in a Recirculating System
Son Maeng Hyun ; Jeon Im Gi ; Jo Jae-Yoon ; Moon HaeYoung ;
Fisheries and aquatic sciences, volume 2, issue 1, 1999, Pages 52~57
Air-drived rotating biological contactor (RBC) system, which is effective method in filtering performance, was tested for the nitrification capacity in a recirculating system. At ammonia concentrations between 0.029 and 0.528 mg/l, the effect of ammonia loading rate on ammonia removal rate at three different hydraulic loading rates could be defined by the following firstorder regression models: Hydraulic loading rate of
, Hydraulic loading rate of
, Hydraulic loading rate of
is ammonia loading rate (mg/l), y is ammonia removal rate
, The equations showed the optimal ammonia removal rate at the hydraulic loading rate of
. Below the ammonia concentration of 2.72 mg/l, first-order regression models between ammonia loading rate and ammonia removal rate at three different rates of speed are defined as follows: Rotational speed of
, Rotational speed of
, Rotational speed of
, where, x is ammonia loading rate (mg/l), y is ammonia removal rate
. The equations show the ammonia removal rate at the rotational speed of 1.0 rpm is significantly higher than that at the rotational speed of either 0.75 rpm or 2.0 rpm (P<0.05).
The Comparison of Two Strains of Fibrocapsa japonica (Raphidophyceae) in New Zealand and Japan
Cho Eun Seob ; Rhodes Lesley L. ; Kim Hak Gyoon ;
Fisheries and aquatic sciences, volume 2, issue 1, 1999, Pages 58~65
Fibrocapsa japonica (Raphidophyceae) is regarded as a harmful algal bloom organism in Japanese waters, where it has been linked to fish kills. Fibrocapsa is a common species in New Zealand coastal waters, particularly in the Hauraki Gulf, where it has regularly bloomed in the spring under E1 Nino climate conditions for the past six years. The New Zealand isolate had 1.4 times more total polyunsaturated acids than the Japanese isolate under the same growth conditions, suggesting that eicosapentaenoic acid in particular coold be used as a discriminating chemotaxonomic marker. The molecular probes tested showed no differential binding of the raphidophytes to lectins, but oligonucleotide probes targeted F. japonica ribosomal RNA bound specifically to both isolates. Neither strain was toxic in mouse or neuroblastoma bioassays. There is no evidence that the New Zealand F. japonica isolates investigated to date produce ichthyotoxins.
Molecular Identification of Gyrodinium impudicum and Gymnodinium sanguineum by Comparing the Sequences of the Internal Transcribed Spacers 1, 2 and 5.8S Ribosomal DNA
Kim Gi Young ; Ha Myoung-Gyu ; Cho Eun Seob ; Lee Tae-Ho ; Lee Sang Jun ; Lee Jae-Dong ;
Fisheries and aquatic sciences, volume 2, issue 1, 1999, Pages 66~77
The sequences coding for the 5.8S rDNA and the internal transcribed spacers (ITS1 and ITS 2) from the isolates of nine isolates of Gyrodinium impudicum and two isolates of Gymnodinium sanguineum species were amplified, sequenced and compared with the previously known Alexandrium species and Gymnodinium catenatum. The genetic distance analyses based on the sequence alignment indicated that Gymnodinium catenatum and Gyrodinium impudicum species were some related, Alexandrium species was distant. G. catenatum and G. sanguineum were quite separate, but these two species belonged to the same genus. G. impudicum and G. catenatum forming the closet cluster showed some variation in the alignment of ITS regions. The length of ITS1 varied more than that of ITS2 and the length of ITS1 and ITS2 was different for each G. impudicum, Gymnodinium and Alexandrium species. Also, the length of ITS1 was shorter than that of ITS2. However, on the sequences of G. sanguineum, the length of ITS1 was longer about 23 nucleotides than that of ITS2. The phylogenetic analysis and rDNA similarity of G. impudicum and G. catenatum
is higher than the that of G. catenatum and G. sanguineum
. It was thought that the phylogenetic analysis and the genetic distance revealed that G. impudicum and G. catenatum were clearly different species and G. impudicum may belong to the genus of Gymnodinium.
Ultrastructural Changes in the Cuticle of Palaemon serrifer (Palaemonidae, Crustacea) during the Intermolt Cycle
Park Jeung Sook ;
Fisheries and aquatic sciences, volume 2, issue 1, 1999, Pages 78~84
Ultrastructural changes in the cuticle of Paleamon serrifer associated with the intermolt cycle were examined and quantified as changes in the cuticular thickness. The cuticular thickness in each zoea stage increased with time elapsed after molting. The cuticle in the premolt stage was about 1.5 and 3 times thicker than that in the postmolt and intermolt stage, respectively. The cuticle in the premolt stage, including the molting space, was more than 5 times as thick as in the postmolt stage. In addition, newly hatched larvae were individually reared in the laboratory and body length for each specimen was measured frequently until the end of zoea VI. An average increase in body length between one zoea stage and the next is about
of the length of the previous stage. Within individual zoea stages, the premolt stage had a body length some 0.3% longer than that of the postmolt stage, indicating a growth rate of about 0.03 mm/d.
Expression Patterns of Heat Shock Proteins in Primary Cultured Hepatocytes from Flounder (Paralichthys olivaceus)
Kim Woo Jin ; Park Doo Won ; Park Jung Youn ; Kang Ho Sung ; Kim Han Do ;
Fisheries and aquatic sciences, volume 2, issue 1, 1999, Pages 85~92
We examined the expression patterns of heat shock proteins in primary cultured hepatocytes from flounder (Paralichthys olivaceus) exposed to heat shock. The expression of hsp90, hsp70, hsp40, hsp30, and hsp27 was induced and major polypeptides were hsp70, hsp30 and hsp27. Northern blot analysis showed that expression of hsp70 was inhibited by transcriptional inhibitor actinomycin D, suggesting that expression of hsp70 gene is regulated at the transcriptional level. Prolonged exposure of cells to an elevated incubation temperature
induced the transient synthesis of hsp90, hsp70, hsp40, and hsp30 whereas maintenance of cells at a slightly higher incubation temperature
induced the continuous syntheses of these hsps. When cells were incubated at a higher temperatures
, the synthesis of hsps was almost similar to that of hsps in cells exposed to 32't except for the induction of hsp27 synthesis. These results that temperature and incubation time for optimum expression of each hsp during prolonged heat shock are different.
Expression of GiIthead Seabream (Sparus aurata) Growth Hormone in Escherichia coli Using Alginate Lyase Gene Promoter of Pseudomonas sp.
Lee Jong-Hee ; Choi Sun-Young ; Lee Sang-Bong ; Jin Cheal-Ho ; Huh Sung-Hoi ; Kong In-Soo ;
Fisheries and aquatic sciences, volume 2, issue 1, 1999, Pages 93~97
The promoter region of alginate lyase gene (aly) from Pseudomonas sp. W7 was used for the high expression of gilthead seabream (Sparus aurata) growth hormone (GH) gene in Esherichia coli. PCR product encoding the premature segment of the growth hormone. was cloned to the downstream of aly promoter. GH was overexpressed With 46 ammo acid of alginate lyase as fusion protein. GH was immunoreactive and production of GH was repressed with supplementation of
glucose into culture media.
Morphological Variation and Genetic Relationship among Populations of the Shortnecked Clam Ruditapes philippinarum Collected from Different Habitats
Kwon Joon Yeong ; Park Ji Won ; Lee Yong-Han ; Park Jung-Youn ; Hong Yong-Ki ; Chang Young Jin ;
Fisheries and aquatic sciences, volume 2, issue 1, 1999, Pages 98~104
The characteristics of the populations of shortnecked clam (Ruditapes philipinarum) originated from three different seed-production sites, Hadong, Kochang and Ulsan along the coast of Korea, were analysed by the morphological differences and the random amplified polymorphic DNA (RAPD) profiles. The morphology of the shell and survival rate for each population were also investigated 13-months after transplantation to a farming site in Shinsung. The morphology of the populations from Hadong and Kochang showed significant differences (P<0.05), but one year after transplantation to Shinsung, the morphology of these three populations was no longer significantly different (P>0.05). The template DNA for RAPD was efficiently extracted from the digestive diverticula of the clams. Up to 13 of amplified fragments were detected using arbitrary primers. Within the species of R. philipinarum, the genetic similarities ranged from 0.196 to 0.259. The populations from Hadong and Ulsan showed the highest similarity. The survival rates of the populations from Hadong
were higher than that from Kochang
13-months after transplantation. From the RAPD analysis, it could be used as one of the primary criterion in determining which shellfish populations among various seed-production sites tend to be genetically similar and more adaptable and transplantable to a farming site.
Isolation of Chitinolytic Bacteria from the Viscera of Korean Bony Fishes and Optimization of the Enzyme Production
Lee Jung-Suck ; Joo Dong-Sik ; Cho Soon-Yeong ; Cho Man-Gi ; Lee Eung-Ho ;
Fisheries and aquatic sciences, volume 2, issue 1, 1999, Pages 105~111
In order to produce functional chitin oligosaccharides, a chitinolytic bacterium was newly screened from the viscera of Korean bony fishes, and identified as Bacillus sp. LJ-25. For the production of chitinolytic enzymes,
nutrient broth and
colloidal chitin were used as nitrogen and carbon source, respectively. The optimal temperature, initial pH and concentration of NaCl for the enzyme production by Bacillus sp. LJ-25 were
, respectively. The enzyme activity of Bacillus sp. LJ-25 increased until the incubation time of 168 hr, followed by a decrease in activity.