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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Fisheries and aquatic sciences
Journal Basic Information
Journal DOI :
The Korean Society of Fisheries and Aquatic Science
Editor in Chief :
Sungchul C. Bai
Volume & Issues
Volume 2, Issue 2 - Dec 1999
Volume 2, Issue 1 - Jun 1999
Selecting the target year
Fatty Acid Compositions of Cultured Oyster (Crassostrea gigas) from Korean and Japanese Spats
Jeong Bo-Young ; Moon Soo-Kyung ; Jeong Woo-Geon ;
Fisheries and aquatic sciences, volume 2, issue 2, 1999, Pages 113~121
Changes in lipid content, lipid class and fatty acid compositions of the cultured oysters in shallow-water, Bukman bay (Tongyeong, Korea), using both Korean and Japanese spats were investigated. The content of non-polar lipid (NL) comprised approximately
of total lipid (TL) in the cultured oysters. There was a positive correlation between NL content and meat weight, y=0.287lx-15.309 (r=0.834l, p<0.001). The prominent fatty acids of the oysters were 16:0, 20:5n-3 (eicosapentaenoic acid, EPA), 22:6n-3 (docosahexaenoic acid, DHA), 18:0, l8:1n-7, l8:1n-9, l6:1n-7, 14:0 and l6:4n-3. During the growth of the oysters, l6:4n-3 showed the highest coefficient of variation, accounting for
for the Korean oyster and
for the Japanese one, respectively. Both oysters showed low level of n-3 fatty acids such as DHA and EPA and high level of n-6 fatty acid, 20:4n-6, in the spawning period (August). During growth of the oysters, both EPA and DHA were the richest fatty acids in the harvest period (December, 314 mg/100g sample) and in the pre-spawning period (July, 237-247 mg/100g sample), respectively. Consequently, the cultured oyster with Japanese spat contained approximately two times more n-3 fatty acids per oyster individual than those with Korean one in the harvest season.
Phylogenetic Relationships of the Family Metridinidae (Copepoda: Calanoida)
Soh Ho Young ; Ohtsuka Susumu ; Suh Hae-Lip ;
Fisheries and aquatic sciences, volume 2, issue 2, 1999, Pages 122~128
Several differences are revealed in the segmentation and setation of the antennule, maxillule, maxilliped and fifth legs between three genera of the family Metridinidae, Metridia, Pleuromamma, and Gaussia. The phylogenetic relationships are cladistically analyzed on the basis of 10 morphological characters. The cladogram shows that Gaussia and Pleuromamma form a sister group, with Metridia which is the first branch to diverge within the family. The presence Of quadritheks on the male antennules of Metridia and Pleuromamma seems to be an important synapomorphy. The loss of quadritheks in Gaussia represents likely evidence of a character reversal into the ancestral condition.
Redescription of Haloptilus caribbeanensis (Copepoda: Calanoida) from the Pacific, with Remarks on the Morphology of Antennules in the Genus Haloptilus
Soh Ho Young ; Suh Hae-Lip ; Ohtsuka Susumu ;
Fisheries and aquatic sciences, volume 2, issue 2, 1999, Pages 129~134
Haloptilus caribbeanensis Park, 1970 (Copepoda, Calanoida, Augaptilidae) is redescribed in detail on the basis of an adult female collected from Suruga Bay, Japan. This is the first record of the species from the Indo-Pacific region. Morphology of the Pacific specimen agrees well with that of the Caribbean Sea and Gulf of Mexico specimens, except for the numbers of mandibular teeth. The former has five teeth and the latter six teeth on mandible. The segmentation and segmental aesthetasc numbers of female antennules of H. caribbeanensis are compared with those of five species of Haloptilus (H. angusticeps, H. fons, H. longicomis, H. ornatus and H. spiniceps). These characters show morphological differentiation at the species level. H. caribbeanensis has no aesthetasc on the proximal segments II, IV, and VI of the female antennules.
Comparison of Extractive Nitrogenous Constituents between the Diploid and the Triploid of Oyster Crassostrea gigas Whole Body
Park Choon-Kyu ;
Fisheries and aquatic sciences, volume 2, issue 2, 1999, Pages 135~141
In order to investigate the composition of extractive nitrogenous components in the diploid and the triploid oysters, Crassostrea gigas, cultured at the south coast of Korea, the whole edible part (whole body) was analyzed into extractive nitrogen, free amino acids, oligopeptides, ATP and its related compounds, quaternary ammonium bases, and guanidino compounds using specimens collected from April to May of 1992. The major free amino acids in the diploid and the triploid were taurine, proline, alanine, glycine, glutamic acid hypotaurine, glutamine, arginine, aspartic acid, and
. There was no conspicuous difference in the constituents of free amino acids between the diploid and the triploid. A lot of hypotaurine was detected in the diploid and the triploid of oyster and the contents of them were 107 mg and 123 mg/100g, respectively. The compounds, glycinebetaine, homarine and trigonelline were found in both the diploid and the triploid. Among them, glycinebetaine was the most prominent in all the samples. The amount of protein, glycogen, extractive nitrogen, oligopeptides, ATP and its related compounds, and free amino acids in the triploid was higher than that of the diploid (p<0.10)
Reproductive Cycle of the Ark Shell, Scapharca subcrenata, on the West Coast of Korea
Kwun Sun-Man ; Chung Ee-Yung ;
Fisheries and aquatic sciences, volume 2, issue 2, 1999, Pages 142~148
Monthly changes in the gonad index (GI), egg-diameter composition, gonadal development, reproductive cycle of the ark shell, Scapharca subcrenata, were investigated by histological method and morphometric data. This species is dioecious and oviparous. The gonad is located among the subregion of mid-intestinal gland, digestive diverticula and the outer fibromuscular layers compacted by the fibrous connective tissues and muscle fibers. The gonad index sharply increased in May, reached the maximum value in June, and then gradually decreased from July to December. The reproductive cycle of this species can be divided into six successive stages: early active stage (January to May), late active stage (June to July), ripe stage (June to August), partially spawned stage (July to September), degenerative stage (August to December), and resting stage (January to April). S. subcrenata spawns once a year between July and early September, and the main spawning occurred between July and August when the water temperatures were above
. This evidence suggest that timings of maturation and spawning are closely related to water temperatures. Even though the spawning period was once a year, it is assumed that the number of spawning frequencies (broods) may occur more than twice during the spawning season.
Properties of Low-Molecular Alginate by Ultrasound
Kim Sang-Moo ; Park Seong-Min ; Lee Keun-Tae ; Bae Tae-Jin ;
Fisheries and aquatic sciences, volume 2, issue 2, 1999, Pages 149~154
Response Surface Methodology was applied for optimizing the processing parameters of ultrasound treatment in order to produce low-molecular alginate. The use of ultrasound significantly reduced viscosity of alginate solutions. Suggested parameters of ultrasound treatment for maximum reduction of alginate molecular weight were: specific intensity, 115.81
at 20kHz frequency; treatment time, 35.55 min; temperature,
; alginate concentration,
. Low-molecular alginate obtained by ultrasound had two peaks on Sepharose CL-6B gel filtration. The viscosities of control, fraction I, and fraction II at
were 3.07, 1.23, and 0.82cps, respectively. Molecular weights of control, fraction I, and fraction II alginates were 336,500, 70,400, and 52,800 daltons, and their solubilities were 3, 6, and
, respectively. The lower molecular weight of alginate, the lower the alcohol precipitation and the higher
ion binding capacities. Heavy metal ion binding capacities of alginates were high in the following order of Pb, Cd, Zn, and Co.
Studies for Reestablishment of Approval Toxin Amount in Paralytic Shellfish Poison-Infested Shellfish -4. Detoxification and Toxin Composition in Paralytic Shellfish Poison-Infested Oyster during Processing-
Jeong Hyun-Jeong ; Shin Il-Shik ; Kim Young-Man ;
Fisheries and aquatic sciences, volume 2, issue 2, 1999, Pages 155~160
Studies on detoxification of Paralytic Shellfish Poison (PSP)-infested oyster, Crassostrea gigas were carried out using available processing resources. Changes of paralytic shellfish toxin components and specific toxicity during canning process were also investigated with high performance liquid chromatography (HPLC). Toxic oysters collected at Hachong in Koje Bay were used for experimental samples. The toxicity of oysters with range of 185-778 ug/100g was reduced below the quarantine limit of 80 ug/100g or not detected level by the mouse bioassay after canning process. The mole
of toxin components in the shucked oyster was in the order of 25.1 mole
of gonyautoxin 1, 19.2 mole
of gonyautoxin 3, 17.2 mole
of gonyautoxin 4 and 14.6 mole
of gonyautoxin 2. This sample had tracing amounts of Cl, C2, saxitoxin and neosaxitoxin. In the case of specific toxicity, the major toxins were consisted of gonyautoxin 1-4. The sum of gonyautoxin 1, 2, 3 and 4 was 80% of total toxicity of oyster. Saxitoxin and decarbamoylsaxitoxin were the more thermostable than any other toxin components.
Effects of Storage Temperature on the Post-Mortem Changes of Wild and Cultured Olive Flounder Muscle
Cho Young Je ; Kim Tae Jin ; Yoon Ho Dong ;
Fisheries and aquatic sciences, volume 2, issue 2, 1999, Pages 161~166
The rigor-mortis progress of cultured olive flounder spiked at the brain started much faster than that of wild one. They attained full rigor state after 30 hrs at
, 36 hrs at
and 50 hrs at
in the cultured flounder, while after 36 hrs at
, 50 hrs at
, and 60 hrs at
in the wild. ATP concentration in the muscle was around
for wild and
for cultured flounder. ATP breakdown progressed rapidly in
samples, followed by
-ATPase activity of myofibrillar protein in the presence of 0.25mM CaCb was higher in cultured myofibri1lar protein than in wild one.
-ATPase activities of myofibrillar protein increased during storage in samples stored at
while decreased in samples stored at
. The level of breaking strength of muscle immediately after death was higher in the wild muscle than in the cultured muscle. The breaking strength reached maximum level at 10 hrs after death in both samples.
LDH/ AChE and LDH/BChE Ratios (Paralichthys olivaceus) as Biomarkers of Coastal Pollution on Coast of Korea.
Choi Jin-Ho ; Kim Dong-Woo ; Kim Chang-Mok ; Yang Dong Beom ;
Fisheries and aquatic sciences, volume 2, issue 2, 1999, Pages 167~171
This study was designed to develop biomarkers of coastal pollution using biochemical indices of flounder (Paralichthys olivaceus) by changes in lactate dehydrogenase (LDH) activity in the serum and cholinesterase activities in brain membranes. For this purpose acetylcholiesterase (AChE) activity, butyrylcholinesterase (BChE) activity, LDH/AChE ratio and LDH/BChE ratio of cultured flounders at 5 different sites on the southern coast of Korea were compared to those of wild flounders caught in the Pohang, eastern coast of Korea as a control group. Relatively high LDH activities were measured in the serum of flounders cultured on the southern coast of Korea (0.101-0.145 unit) than those in the Pohang control group (0.093 unit). AChE activities were significantly low
in brain membranes of cultured flounders compared to those in the Pohang control group. The ratios of LDH/AChE and LDH/BChE were consistently higher
in cultured flounders than those of Pohang control group. Thus, we propose that the ratios of LDH/AChE and LDH/BChE in flounders could be applicable for the diagnosis of marine pollution.
Application of Image Analysis System for Red Tide Organisms
Cho Eun Seob ; Kang Yoon Mi ; Kim Gwang Hoon ;
Fisheries and aquatic sciences, volume 2, issue 2, 1999, Pages 172~175
Relative DNA contents in some harmful algae were measured using DAPI staining and image analysis system. This method was useful to identify some morphologically similar species and isolates from harmful algal blooms (HABs). In exponential phase, Prorocentrum micans had higher relative DNA content (RD) of
than any other isolates, followed by Cochlodinium polykrikoides
, Scrippsiella trochoidea
and P. minimum
. When they were fixed with Lugol's solution, it was difficult to d,iscern C. polykrikoides from G. impudicum under the light microscope, but the DNA contents were quite different in two species. C. polykrikoides contained about twice as much RD as G. impudicum under the same culture conditions and exponential phase. DAPIstained DNA feature in C. polykrikodes showed concentrated in the peripheral part of the cell, but in G. impudicum showed a compact structure in the central part. Although A. tamarense and S. trochoidea were morphologically similar under the light microscope, nuclear DNA content of A. tamarense was twice as much as that of S. trochoidea.
Variations of Gonadotropin Subunits mRNA Levels at Different Stages of Ovarian Development in Masu Salmon, Oncorhynchus masou
Kim Dae-Jung ; Han Chang-Hee ; Aida Katsumi ;
Fisheries and aquatic sciences, volume 2, issue 2, 1999, Pages 176~181
The variations of gene expression and pituitary contents of GTH subunits during the ovarian development of masu salmon, Oncorhynchus masou, were investigated. The pituitary GTHs contents was measured by radioimmunoassays (RIAs) using purified GTH subunits and their antibodies. Pituitary contents of GTH
gradually increased from April through August, and reached the maximum in October. On the other hand, pituitary contents of GTH
remained low until August, but they rapidly increased in October. Total RNAs were prepared from pooled pituitaries and the GTH subunits mRNA in pituitaries was quantified by Northern blot hybridization using masu salmon cDNA probes for each GTH subunit. GTH
mRNA level increased with the progression of ovarian maturity. However, GTH
mRNA was detected only at a more advanced stage, and were extremly high at ovulation. A high levels for GTH a mRNA was detected only at ovulation stage. The synchronous increase in pituitary contents and mRNA levels suggested that ovarian maturity in masu salmon was regulated by both GTH I and GTH II.
Production of extracellular polysaccharide by Monilinia fructigena for aquaculture
Kwak Jung-Ki ; Park Jin-Hee ; Lee Jung-Suck ; Goetz Peter ; Cho Man-Gi ;
Fisheries and aquatic sciences, volume 2, issue 2, 1999, Pages 182~188
Production of extracellular polysaccharide by Monilinia fructigena in B-I medium containing cereals was higher than that in glucose medium. Productivities in B-I medium and glucose medium were 0.7g/l nd 0.2-03g/l respectively. The maximum content of polysaccharide occurred at the rising point from the lowest pH of culture. As the apparent viscosity of the polysaccharide solution increased, the flow Index (m) decreased, and the consistency Index (Kc) also increased. The polysaccharide solution was a typical pseudoplastic fluid. The mycelium was separated from the culture solution by
mesh-filter and the polysaccharide was precipitated by adding 50% of ethanol (v/v). The amount of the polysaccharide removed from the filtrated solution was 0.45 g/l and the amount adhered to the mycelium was 0.25g/l. In experiments for investigating growth enhancement of rotifer (Brachionus plicatilis) by the polysaccharide, the dose of the polysaccharide was 1mg per 10,000 organisms of rotifer. Maximum specific growth rate of rotifer with feed consisting of sea Chlorella sp. and the polysaccharide was 1.095/day in the batch culture for 10 days. A semi-continuous culture was done for 30 days, the biomass of rotifer could be harvested twice. Maximum specific growth rate with sea Chlorella sp. and the polysaccharide was 0.734/day before the first harvest, and 1.685/day before the second harvest. Productivity was 38
with sea Chlorella sp. and the polysaccharide.
New Records on Three Harpacticoid Copepods Associated with Marine Macroalgae in Korea
Song Sung Joon ; Yun Sung Gyu ; Chang Cheon Young ;
Fisheries and aquatic sciences, volume 2, issue 2, 1999, Pages 189~198
Three harpacticoid copepods, Dactylopodamphiascopsis latifolius (Sars, 1909), Diosaccus ezoensis Ito, 1974 and Ambunguipes rufocincta (Brady, 1880) are recorded as new to the Korean fauna with illustrations, on the basis of the specimens collected mainly from the marine macro algae during the period from June 1990 to September 1997.
Long-term Environmental Changes and the Interpretations from a Marine Benthic Ecologist's Perspective (I) - Physical Environment
Yoo Jae-Won ; Hong Jae-Sang ; Lee Jae June ;
Fisheries and aquatic sciences, volume 2, issue 2, 1999, Pages 199~209
Before investigating the long-term variations in macrobenthic communities sampled in the Chokchon macrotidal flat in Inchon, Korea, from 1989 to 1996, we need to understand how environmental factors in the area vary. As potential governing agents of tidal flat communities, abiotic factors such as mean sea level, seawater, air temperature, and precipitation were considered. Data for these factors were collected at equal intervals from 1976 or 1980 to 1996, and were analyzed using a decomposition method. In this analysis, all the above variables showed strong seasonal nature, and yielded a significant trend and cyclical variation. Positive trends were seen in the seawater and air temperatures, and based upon this relationship, it was found that the biological sampling period of our program has been carried out during warmer periods in succession. This paper puts forth some hypotheses concerning the response of tidal flat macrobenthos communities to the changing environment including mild winters in succession.
Long-term Environmental Changes: Interpretations from a Marine Benthic Ecologist's Perspective (II) -Eutrophication and Substratum Properties
Yoo Jae-Won ; Hong Jae-Sang ; Lee Jae June ;
Fisheries and aquatic sciences, volume 2, issue 2, 1999, Pages 210~217
Chemical oxygen demand (COD), phytoplankton cell number and chlorophyll-a concentration (Chl-a), sediment mean grain size and ignition loss were studied to determine their temporal trends in the study area. Historical data of COD, cell number and Chl-a were gathered from the late 1960s or early 1980s to 1997, and trends in temporal domain were obtained from a simple regression. Sediments for grain size and ignition loss (as organic contents in sediments) were sampled from the Chokchon macrotidal flat bimonthly from September 1990 to November 1996, and were analyzed using the decomposition method of time series analysis. In general, the first three data showed increasing trends based on regression analysis. The trends of sediment grain size fluctuated in a neutral pathway while those of ignition loss yielded no increasing pattern. In contrast with the suggestions from Ahn and Choi (1998) who reported a coarsening variation in sediment grain size to be a cause of the directional and remarkable changes of macrofaunal communities in this area, we could not find such a corresponding variation pattern from our samples. In diagnosing eutrophication, a paradoxical phenomenon was encountered between the trends in water column (COD, cell number and Chl-a) and sediment (ignition loss) data. In this paper, we inferred the possible processes that produce the discrepancy. Some explanations and biological responses to eutrophication were predicted and discussed.
Purification and Characterization of Protease from the Hepatopancreas of Shrimp, Penaeus orientalis
Oh Eun-Sil ; Kim Doo-Sang ; Choi Sung-Mi ; Kim Jeong-Han ; Pyeun Jae-Hyeung ; Cho Deuk-Moon ; Kim Hyeung-Rak ;
Fisheries and aquatic sciences, volume 2, issue 2, 1999, Pages 218~225
A protease without tryptic and chymotryptic activities was purified from the hepatopancreas of shrimp, Penaeus orientalis, using Q-Sepharose ionic exchange, benzamidine Sepharose-6B affinity, Mono-Q, and gel chromatography. Molecular weight (M.W.) of the protease was estimated to be 27kDa by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDSPAGE). The amino acid composition of the protease was different from that of protease from P. japonicus or trypsin from P. orientalis. The protease was completely inhibited by benzamidine,
chloromethyl ketone (TLCK), and phenylmethylsulfonyl fluoride (PMSF) and was not affected by leupeptin, pepstatin, N-tosyl-L-phenylalanine chloromethyl ketone (TPCK), iodoacetate, and ethylenediamine tetra acetate (EDTA). The enzyme did not have any activity against Na-benzoyl-DL-arginine p-nitroanilide (BAPNA) or N-benzoyl-L-tyrosine ethyl ester (BTEE) which are specific substrates of trypsin and chymotrypsin, respectively. However, the protease showed hydrolytic activity for a carboxyl terminal of Tyr, Trp, Phe, Glu, and Cys.