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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Fisheries and aquatic sciences
Journal Basic Information
Journal DOI :
The Korean Society of Fisheries and Aquatic Science
Editor in Chief :
Sungchul C. Bai
Volume & Issues
Volume 4, Issue 4 - Dec 2001
Volume 4, Issue 3 - Sep 2001
Volume 4, Issue 2 - Jun 2001
Volume 4, Issue 1 - Mar 2001
Selecting the target year
Morphological Feature Extraction of Microorganisms Using Image Processing
Kim Hak-Kyeong ; Jeong Nam-Su ; Kim Sang-Bong ; Lee Myung-Suk ;
Fisheries and aquatic sciences, volume 4, issue 1, 2001, Pages 1~9
This paper describes a procedure extracting feature vector of a target cell more precisely in the case of identifying specified cell. The classification of object type is based on feature vector such as area, complexity, centroid, rotation angle, effective diameter, perimeter, width and height of the object So, the feature vector plays very important role in classifying objects. Because the feature vectors is affected by noises and holes, it is necessary to remove noises contaminated in original image to get feature vector extraction exactly. In this paper, we propose the following method to do to get feature vector extraction exactly. First, by Otsu's optimal threshold selection method and morphological filters such as cleaning, filling and opening filters, we separate objects from background an get rid of isolated particles. After the labeling step by 4-adjacent neighborhood, the labeled image is filtered by the area filter. From this area-filtered image, feature vector such as area, complexity, centroid, rotation angle, effective diameter, the perimeter based on chain code and the width and height based on rotation matrix are extracted. To prove the effectiveness, the proposed method is applied for yeast Zygosaccharomyces rouxn. It is also shown that the experimental results from the proposed method is more efficient in measuring feature vectors than from only Otsu's optimal threshold detection method.
Reconstruction and Elimination of Optical Microscopic Background Using Surface Fitting Method
Kim Hak-Kyeong ; Kim Dong-Kyu ; Jeong Nam-Soo ; Lee Myung-Suk ; Kim Sang-Bong ;
Fisheries and aquatic sciences, volume 4, issue 1, 2001, Pages 10~17
One serious problem among the troubles to identify objects in an optical microscopic image is contour background due to non-uniform light source and various transparency of samples. To solve this problem, this paper proposed an elimination method of the contour background and compensation technique as follows. First, Otsu's optimal thresholding method extracts pixels representing background. Second, bilinear interpolation finds non-deterministic background pixels among the sampled pixels. Third, the 2D cubic fitting method composes surface function from pivoted background pixels. Fourth, reconstruction procedure makes a contour image from the surface function. Finally, elimination procedure subtracts the approximated background from the original image. To prove the effectiveness of the proposed algorithm, this algorithm is applied to the yeast Zygosaccharomyces rouxii and ammonia-oxidizing bacteria Acinetobacter sp. Labeling by this proposed method can remove some noise and is more exact than labeling by only Otsu's method. Futhermore, we show that it is more effective for the reduction of noise.
Seasonal Variations in Biochemical Components of the Visceral Mass and Adductor Muscle in the Pen Shell, Atrina pectinata
Baik Sung-Hyun ; Kim Kang-Jeon ; Chung Ee-Yung ; Choo Jong-Jae ; Park Kwan Ha ;
Fisheries and aquatic sciences, volume 4, issue 1, 2001, Pages 18~24
Seasonal content changes of the three key nutrients for organisms, protein, lipid and glycogen, were analyzed for a whole year to delineate the seasonal energy strategy in pen shells, Atrina pectinata. Two metabolically important organs, the visceral mass and the posterior adductor muscle, were examined. Protein in the visceral mass rose in April and stayed at the level until June followed by the constant minimum value between August and November. The protein contents in the posterior adductor muscle increased sharply in April and again in July, followed by a gradual decline thereafter. Total lipid contents in the visceral mass gradually increased between January and May, and then slowly decreased until September since which a new weak increase was noticed. Lipid levels in the adductor muscle rapidly dropped in June and July. Glycogen contents in the visceral mass rapidly increased between February and June, followed by a drastic drop in July. This reduced visceral glycogen level was maintained up to September, and a gradual reduction ensued. Glycogen contents in the adductor muscle steadily but markedly increased from April reaching the maximum in August, and then slowly declined thereafter. These results suggest that an accelerated protein and lipid synthesis occurs in the gonad when the pen shell undergoes the ripe stage of gametogenesis, but the levels of these two nutrients decrease on spawning. With this gonadal process, regular protein synthesis and lipid storage in the posterior adductor muscle are temporarily arrested. The most important nutrient reserves that support gonad developmental cycles in a long term seem to be glycogen of the posterior adductor muscle.
Molecular Identification of the Fish 4-Aminobutyrate Aminotransferase from Flounder, Paralichthys olivaceus
Sung Bo Kyung ; Kim Young Tae ;
Fisheries and aquatic sciences, volume 4, issue 1, 2001, Pages 25~31
4-Aminobutyrate aminotransferase plays an essential role in the 4-aminobutyric acid shunt, converting 4-aminobutyrate to succinic semi aldehyde. We isolated and sequenced' a fish cDNA fragment that encodes 4-aminobutyrate aminotransferase. A brain cDNA library from flounder (Paralichthys olivaceus) was constructed using the ZAP- III XR vector and screened for the fish 4-aminobutyrate aminotransferase gene using a probe derived from the conserved sequences of known mammalian 4-aminobutyrate aminotransferases. A partial cDNA for 4-aminobutyrate aminotransferase was cloned and found to be 700 bp in length corresponding to 66 amino acids. Nucleotide sequence of the clone was aligned with NCBI (National Center for Biotechnology Information) DNA sequence data base. The result showed high sequence identity with previously reported mammalian 4-aminobutyrate aminotransferases. The transcriptional level of flounder 4-aminobutyrate aminotransferase was detected with the presence of mRNA at different flounder tissues by reverse transcription-polymerase chain reaction (RT-PCR). The expression of flounder 4-aminobutyrate aminotransferase was also tested and detected from the flounder tissues of the brain, liver, kidney and pancreas.
Comparison of IHNV Detection Limits by IMS-RT-PCR, Western Blot and ELISA
Kim Soo-Jin ; Lee Eun-Young ; Oh Myung-Joo ; Choi Tae-Jin ;
Fisheries and aquatic sciences, volume 4, issue 1, 2001, Pages 32~38
Several molecular biological techniques have been used to detect virus rapidly and accurately, but these methods have limitations in the early stage of viral infection with very low concentration of virus. We compared the detection limits of IMS-PCR, Western blot and ELISA with infectious hematopoietic necrosis virus OHNV). Four antibodies, rabbit anti-IHNV polyclonal antibody, anti-IHNV nucleocapsid protein monoclonal antibody, anti-IHNV nucleocapsid protein polyclonal antibody, and anti-IHNV glycoprotein polyclonal antibody, were tested to find out the most effective antibody for each method. The detection limit with IMS- PCR was
pfu when the viral RNA was extracted before RT-PCR. In the western blot with rabbit antiIHNV polyclonal antibody one pfu of virus could be detected. In ELISA, 10 pfu of virus particles were detected with the same antibody.
Effect of Transgenic Genotype on Transgene Expression in Mud Loach (Misgurnus mizoIepis): I. Copy Number-Dependent Expression in Gynogenetically Derived Homozygous Transgenics
Nam Yoon Kwon ; Noh Jae Koo ; Kim Dong Soo ;
Fisheries and aquatic sciences, volume 4, issue 1, 2001, Pages 39~46
To examine the effect of copy number-dependent transgenic genotype on the expression of foreign gene, stable hemizygous and homozygous transgenic breeding line was established using artificial parthenogenesis. For this purpose, induced diploid gynogenetic transgenesis was optimized in mud loach (Misgurnus mizolepis) using UV-irradiated cyprinid loach (M. anguillicaudatus) sperm and thermal shocks. Optimum UV range for inactivation of cyprinid loach sperm was between 3,150 to
The UV-irradiated sperm were inseminated into eggs from recessive color strain (yellow) or heterozygous transgenic mud loach containing CAT gene. Cold shock at
for 60 min, 5 min post fertilization successfully restored the diploidy of eggs inseminated with UV-irradiated sperm. Restoration to diploidy was confirmed by flow cytometry and gynogenetic status was verified by examining maternal exclusive inheritance of multi-locus DNA fingerprints, body color and transgenic marker. Putative isogenic transgenic fish clearly showed homozygous status at trans gene locus based on Southern blot hybridization and progeny testing. Further, such homozygous gynogenetic diploids revealed the increased levels of transgene expression, when compared to those of heterozygous (hemizygous) transgenic fish.
Nitrite Scavenging Activity of Bromophenol Congeners from Symphyocladia latiuscula
Park Hye Jin ; Lee Hee Jung ; Jung Hyun Ah ; Choi Jae Sue ;
Fisheries and aquatic sciences, volume 4, issue 1, 2001, Pages 47~49
Nitrite scavenging activity of a methanol extract of Symphyocladia latiuscula was studied. The methanol extract scavenged the nitrite in a dose-dependent manner. The MeOH extract was then sequentially partitioned with n-hexane,
, EtOAc, n-BuOH and
. The scavenging activity of the fractions increased in order of
, n-hexane, EtOAc, n-BuOH, and
. Especially, the activity of the
fraction was comparable to that of L-ascorbic acid. Column chromatography of the most active
fraction over silica gel yielded three active bromophenol congeners (1-3) which were identified as (2R)-2-(2,3,6-tribromo 4,5-dihydroxybenzyl) cyclohexanone (1), 2,3,6-tribromo 4,5-dihydroxybenzyl methyl ether (2), and 2,3,6tribromo 4,5-dihydroxybenzyl alcohol (3) respectively.