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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Fisheries and aquatic sciences
Journal Basic Information
Journal DOI :
The Korean Society of Fisheries and Aquatic Science
Editor in Chief :
Sungchul C. Bai
Volume & Issues
Volume 4, Issue 4 - Dec 2001
Volume 4, Issue 3 - Sep 2001
Volume 4, Issue 2 - Jun 2001
Volume 4, Issue 1 - Mar 2001
Selecting the target year
Effect of Perilla Oil in Diet on the Biochemical Property of Cultured Sweet Smelt Plecoglossus altivelis
Jeong Bo-Young ; Jeong Woo-Geon ; Moon Soo-Kyung ; Maita Masashi ; Ohshima Toshiaki ;
Fisheries and aquatic sciences, volume 4, issue 4, 2001, Pages 163~170
The effect of perilla oil added in diet on the biochemical properties of cultured sweet smelt, Plecoglossus altivelis, was investigated. The cultured fish were fed two different diets for 8 weeks; a control diet was a commercial diet, which was low in the content of docosahexaenoic acid (DHA, 22: 6n-3) and eicosapentaenoic acid (EPA, 20: 5n-3) less than approximately
(CO group) and an experimental diet (PO group) was added perilla oil as a lipid source in the diet of the CO group. The PO group was superior in growth rate and feed efficiency compared with CO group. This trend showed markedly in female of both groups. The fatty acid composition in the muscle of PO group was closely related with those of the diet, while those of CO group were not. For plasma components, total cholesterol (CHOU of PO group was higher than that of CO group. Thiobarbituric acid-reactive substances (TBARS), hydroxyl (OH) radical levels and superoxide dismutase (SOD) activity of plasma were higher in PO group than CO group. The intensity of watermelon-like or cucumber-like aroma was much stronger in PO group with higher level of TBARS and OH radical in plasma compared CO group. Survival rate was also high in PO group with high levels of phagocytic rate, CHOL and SOD activity. These results suggest that perilla oil might be usefulness as a lipid source of the cultured sweet smelt diet, in which result in high quality of the cultured fish.
Level in Diet on the Biochemical Property of Cultured Sweet Smelt, PIecoglossus altivelis
Moon Soo-Kyung ; Jeong Woo-Geon ; Kim In-Soo ; Jeong Bo-Young ; Maita Masashi ; Ohshima Toshiaki ;
Fisheries and aquatic sciences, volume 4, issue 4, 2001, Pages 171~179
The effect of
level in diet on the biochemical property of sweet smelt, Plecoglossus altivelis, was investigated. The cultured sweet smelt fish were fed two different diets for 8 weeks; a control diet was added
(CO group) and an experimental diet was added
(HT group). Both diets were rich in docosahexaenoic acid (DHA, 22:6n-3) and eicosapentaenoic acid (EPA, 20:5n-3), accounting for
, respectively. Growth rate (GR) and feed efficiency (FE) were almost no difference between both groups, but female fish of both groups were superior to male fish in GR and FE. Lipid contents of muscle and ovary were high in CO group compared with HT group, but that of testis showed a similar level in both groups. The proportion of polyunsaturated fatty acids (PUFA) in muscle showed almost no difference in sex and between both groups. In testes, the proportion of PUFA was 1.35 times for HT group to 1.54 times for CO group as much as in ovaries, in which was high approximately 1.5 times compared with muscle. Thiobarbituric acid-reactive substances (TBARS) and hydroxyl (OH) radical levels of plasma were higher in CO group than HT group and superoxide dismutase activity was also slightly high in the former. The intensity of watermelon-like or cucumber -like aroma was related positively with TBARS and OH radical levels in plasma. The level of triglyceride (TG) and total cholesterol (CHOL) in plasma of CO group was higher than those of HT group. Survival rate was high in CO group with higher level of TG and CHOL in plasma.
Aluminium and Cadmium Interfere with the Estrogen Receptor Level in the Primary Culture of Hepatocytes in the Rainbow Trout Oncorhynchus mykiss
Hwang Un-Gi ;
Fisheries and aquatic sciences, volume 4, issue 4, 2001, Pages 180~185
Al and Cd-induced inhibition of vitellogenin (VTG) production was examined at the estrogen receptor (ER) level in rainbow trout Oncorhynchus mykiss hepatocytes. The binding of
to hepatocytes reached a plateau 3 days after addition of
to the medium. The binding activity was linearly reduced with the increased concentrations
of 4-hydroxy-tamoxifen (4-OHT) and specific binding linearly increased with the increased doses of
, indicating that the radioligand bound to ER. Al
as well as 4-OHT
significantly reduced the
-binding activity by
, while they completely inhibited VTG production. Al and Cd had no effect on
binding activity at any concentrations used
. These results suggested that Al and Cd inhibited VTG production in part by interfereing with the ER level. Inhibitory effects of these metals on the
upregulation of ER activity are also discussed.
Vitellogenin and Its mRNA Induction by
in the Primary Culture of Hepatocytes in the Rainbow Trout, Oncorhynchus mykiss
Hwang Un-Gi ;
Fisheries and aquatic sciences, volume 4, issue 4, 2001, Pages 186~191
Vitellogenin (VTG) and VTG mRNA induction by
were examined in the primary cultures of hepatocyte in the rainbow trout. Hepatocytes were precultured for 2 days, then
was added and cultured for another 5 days. Media and hepatocytes were then analyzed by electrophoresis and Northern blotting for VTG and VTG mRNA, respectively. The hepatocytes were formed a few aggregates within 5 days without further spreading to a monolayer. Cell viability and high DNA content were maintained during the incubation. The hepatocyte culture with E2 induced a weak VTG band at a molecular weight of 175kDa on Day 2 after
addition. The relative amount of VTG was expressed in percentage of total protein concentrations. VTG was gradually increased as
on Day 2,
on Day 4 and
on Day 5. VTG mRNA band was detected at about 6.6 kb in the culture with
at day 1 of culture. The level of VTG mRNA expression linearly increased with time until Day 5 (r=0.97).
Molecular Studies on the Disease Resistance Gene, Proopiomelanocortin (POMC), from Flounder (Paralichthys olivaceus)
Kim Hyun Woo ; Kim Young Tae ;
Fisheries and aquatic sciences, volume 4, issue 4, 2001, Pages 192~196
Proopiomelanocortin (POMC) plays an essential role in the disease resistance system and is the precursor protein of biologically active peptides such as adrenocorticotropin (ACTH),
. We have isolated and sequenced two different forms of POMC cDNA, POMC-I and POMC-II, from a pituitary cDNA library of flounder. POMC-I cDNA consisted of 956 bp corresponding to deduced amino acids of 216 residues and POMC-II cDNA was 982 bp in length corresponding to 194 amino acids, respectively. The results of deduced amino acids analysis of the clones showed high sequence homology with previously reported POMCs amino acid sequences from various species. The homology between flounder POMC-I and -II is
identity. We also constructed a phylogenetic tree based on POMC amino acid sequences.
Correlation between Pharmacokinetics of Praziquantel and Extermination of Microcotyle sebastis (Monogenea) in Cultured Rockfish Sebastes schlegeli
Kim Chun Soo ; Kim Ki Hong ;
Fisheries and aquatic sciences, volume 4, issue 4, 2001, Pages 197~200
To investigate the re-treatment time of Microcotyle sebastis by oral administration of praziquantel, the residue levels of praziquantel in plasma of rockfish Sebastes schlegeli administered orally at a dose of 200 mg/kg B.W. were analyzed by reversed-phase HPLC, and the concentrations of praziquantel in the plasma were correlated with the extermination of M. sebastis. The absorption and depletion of praziquantel in the blood of rockfish were fast and the residual concentrations of praziquantel declined below
within 24 hr post treatment. Most of worms were exterminated within 3 hr after oral administration of praziquantel, however, a small number of M. sebastis were not killed by the treatment until end of the experiment. Considering fast drop of praziquantel in blood and extermination pattern of M. sebastis in the present results, retreatment at an interval of 9-12 hr would be effective for eradication of M. sebastis.
Distribution of Organophosphorus Pesticides in some Estuarine Environments in Korea
Yu Jun ; Lee Dong Ho ; Kim Kyung Tae ; Yang Dong Beom ; Yang Jae Sam ;
Fisheries and aquatic sciences, volume 4, issue 4, 2001, Pages 201~207
To study the distribution of organophosphorus pesticides which are extensively used for agriculture in Korea. Sea water samples were taken from 4 coastal areas during May and August of 1997 and sediment samples were collected from two coastal areas in August of 1997. These samples were analyzed using a Gas Chromatography/Nitrogen Phosphorus Detector (GC/NPD). In August the most commonly found organophosphorus pesticides in the surface waters of Kunsan area were IBP < S-Benzyl O,O-di-isopropyl phosphorothioate >
and EDDP < O-ethyl S,S-diphenyl phosphorodithioate >
which are largely used between June and September to prevent rice blast disease. In Danghang Bay, dry fields located near the mouth of the estuary seemed to affect the concentrations of certain organophosphorus pesticides in the surface waters. Since organophosphorus pesticides applied in the watershed are rapidly decomposed while being transported along freshwater streams, watershed size is not proportional to the concentrations of these pesticides in the coastal waters. Pesticides concentrations measured in August were compared with those in May. IBP concentrations in coastal waters were about an order of magnitude higher in August than in May. Temporal and geographical distribution of individual organophosphorus pesticides is likely to be affected by types of agricultural practices in the watershed. Chloropyrifos was the most important of the organophosphorus pesticides in the sediments of the study area because of its persistent nature and high affinity to particulates.
Gonadal Maturation and Artificial Spawning of the Manila Clam, Ruditapes philippinarum (Bivalvia: Veneridae), in Komso Bay, Korea
Chung Ee-Yung ; Hur Sung Bum ; Hur Young-Baek ; Lee Jung Sick ;
Fisheries and aquatic sciences, volume 4, issue 4, 2001, Pages 208~218
We have investigated the gonad index (GI), gonadal development, reproductive cycle, first sexual maturity, sex ratio, the number of spawned eggs and spawning frequency of the Manila clam, Ruditapes philippinarum. Samples were collected from the intertidal zone of Komso Bay, Korea from January to December in 1999. Monthly changes in the gonad index (GI) and condition index showed a similar pattern in the reproductive cycle. The spawning period was once a year between early June and early October, there was a spawning peak between July and August when seawater temperature was over
. The reproductive cycle of this species can be categorized into five successive stages; early active (February to March), late active (April to May), ripe (April to August), partially spawned (June to October), and spent/inactive stage (August to March). Percentages of first sexual maturity of female and male clams of l5.1-20.0mm in shell length were
, respectively, and
for the clams >25. mm. The sex ratio of individuals >15.1 mm in shell length was about 1:1 $(\chi^2= 0.02,\;p>0.05)$. Number of the eggs released from each clam by the induction increased as the size of clam in terms of shell length increased. Mean number of the eggs from the second induction of the spawning was
of the number of the eggs released in the first spawning. Our data indicated that R. philippinarum in Komso Bay has one major spawning peak with over two minor spawning, and the interval of each spawning was estimated to be approximately 15-17 (average 16.5) days.
Monitoring of Polycyclic Aromatic Hydrocarbons in Sediments and Organisms from Korean Coast
Moon Hyo-Bang ; Choi Hee-Gu ; Kim Sang-Soo ; Jeong Seung-Ryul ; Lee Pil-Yong ; Ok Gon ;
Fisheries and aquatic sciences, volume 4, issue 4, 2001, Pages 219~228
Surface sediments (0- 5 cm), mussels (Mytilus coruscus and M. edulis) and oysters (Crassostrea gigas) were sampled at 20 stations in Korean coast during a period of February to July 2000. Samples were analyzed for polycyclic aromatic hydrocarbons (PAHs) content using gas chromatography coupled to mass spectrometer detector (GC/MSD). The sediment
concentration varied from 7 to 1,214 ng/g dry weight and the level of carcinogenic PAHs of six species ranged from 1 to 563 ng/g dry weight in surface sediments. In organisms, the levels of
were in the range of 63-876 ng/g dry weight and the concentrations of carcinogenic PAHs of six species were in the range of 4-582 ng/g dry weight. The highest PAH concentrations in sediments and organisms were in samples from Station 5 in Pohang coast and Station 8 in Jinhae coast, respectively.
concentrations in sediments and organisms collected from Korean coast were slightly low or comparable to those in other countries. The contribution of ring aromatic groups to sum of 16 PAHs in sediments and organisms showed a similar pattern for most stations. In sediments, the predominant contributions were four and five ring aromatics like fluoranthene, pyrene, benzo[a]anthracene, chrysene, benzo[b]fluoranthene, benzo[k]fluoranthene and benzo[a]pyrene. In the organisms, four ring aromatics like fluoranthene, pyrene, benzo[a]anthracene and chrysene were predominant PAH species. Several molecular indices such as phenanthrene/anthracene, fluoranthene/pyrene and LMW/HMW ratio were used to identify the origin of PAH contamination in sediments. The results indicate that PAHs of Korean coast were mainly of pyro1ytic contaminants with slight petrogenic input. A high correlation within individual PAH compound in marine sediments was observed, while correlation in organisms showed a highly significant relationship for heavier molecular weight PAHs.
Quality Properties of Seasoned-Dried Pacific Saury Treated with Liquid Smoke -1. Volatile Flavor Compounds in Commercial Liquid Smokes-
Park Sung-Young ; Kim Hun ; Cho Woo-Jin ; Lee Young-Mi ; Lee Jung-Suck ; Cha Yong-Jun ;
Fisheries and aquatic sciences, volume 4, issue 4, 2001, Pages 229~237
In order to identify of volatile flavor compounds and polycyclic aromatic hydrocarbons (PAHs) in commercial liquid smokes, this study was conducted to analyze volatile flavor compounds by solvent extraction and/or Purge & Trap method/GC/MSD. A total of 156 volatile flavor compounds were detected in 6 commercial liquid smokes, and these compounds were composed mainly of 12 aldehydes, 60 ketones, 7 alcohols, 14 acids, 20 esters, 24 aromatic compounds, 7 furans and 12 miscellaneous compounds. Ketones
and aromatic compounds
were more abundant than others. The PAHs known as carcinogen have not been detected in this study. The acids
was identified in relatively high concentration compared to other groups. Phenol and its derivatives among aromatic compounds were in relatively high concentration. Especially, the phenol and its derivatives including o-cresol, guaiacol, 4-ethylguaiacol and syringol were in higher concentration.
Quality Properties of Seasoned-Dried Pacific Saury Treated with Liquid Smoke -2. Processing Conditions for Seasoned-Dried Pacific Saury Treated with Liquid Smoke-
Cha Yong-Jun ; Park Sung-Young ; Jeong Eun-Jeong ; Chung Yeon-Jung ; Kim So-Jung ;
Fisheries and aquatic sciences, volume 4, issue 4, 2001, Pages 238~245
Optimal conditions for processing. of seasoned-dried Pacific saury treated with liquid smoke (T2) were evaluated by physicochemical and microbial experiments and sensory evaluation, comparing with control (seasoning only, C) and treatment I
. Two hrs of seasoning time was set, and 23 hrs of drying time was determined in all samples. Finally, T2 product was made by soaking treatments (three times of 1 sec, 8 sec and 1 sec) in
(v/v) liquid smoke (Scansmoke PB 2110) after 30 min, 4 hrs and 22 hrs of hot-air drying, respectively. The histamine contents in 3 seasoned-dried products were in a 15.33-26.99 mg/l00g range. The water activities of 3 seasoned-dried products were 0.719-0.735 range, and the pH of T2 was lower than the others. In the comparison of POV and TBA values among products, the TBA values and POV of Tl and T2 were significantly low compared to C, and also the viable cell counts of T2 was relatively lower than those of the others. In the color values, significant changes were not found among products, and in the sensory evaluation for odor, taste and overall acceptance, T2 had relatively higher preference on the whole items.
Quality Properties of Seasoned-Dried Pacific Saury Treated with Liquid Smoke during Storage -3. Changes in Fatty Acid and Taste Compounds of Seasoned-Dried Pacific Saury Treated with Liquid Smoke During Storage-
Park Sung-Young ; Cho Woo-Jin ; Kim Hun ; Kim So-Jung ; Cha Yong-Jun ;
Fisheries and aquatic sciences, volume 4, issue 4, 2001, Pages 246~251
As a series of studies on improving quality of seasoned-dried Pacific saury, fatty acid compositions and taste compounds of the seasoned-dried saury treated with liquid smoke (T2 product) were examined during storage, comparing with control (C, seasoning only). In the both samples, the major fatty acids were 22:6n-3, 16:0, 22: In-11I, 20: In-11, 18: In-9, 14:0, 20:5 n-3 and 16:1n-7. The contents of saturated fatty acids in C and T2 increased with increasing storage period, while those of polyunsaturated fatty acids decreased. After drying, the contents of 7 non-volatile organic acids contents detected in this study decreased, and the others of non-volatile organic acids, except for malic and citric acids, in both C and T2 decreased with storage period. The contents of nucleotides and their related compounds in both C and T2 decreased with increasing storage period. The content of total free amino acids in raw sample was 556.96 mg/100g and increased up to 895.77 mg/l00 g and 958.40 mg/100g in C and T2, respectively, after drying, and total contents of free amino acids in both C and T2 somewhat decreased after 60 days of storage.
Bactericidal Activity of Sawa-wasabi (Wasabia japonica) Against the Fish Pathogenic Bacteria
Shin Il-Shik ;
Fisheries and aquatic sciences, volume 4, issue 4, 2001, Pages 252~256
In this study, the bactericidal activity of each extract from Sawa-wasabi (Wasabia japonica) root, stem and leaf against the fish pathogenic bacteria were examined. The main component related to bactericidal activity in Sawa-wasabi was well known to AlT. The Sawa-wasabi roots showed the highest AIT amount with 1.18 mg/g. Stems was 0.41 and leaves was 0.38 mg/g. All of them showed bactericidal activity against 2 strains of Vibrio hollisae, V. anguillarum, and 2 strains of Edwardsiella tarda, but weak effect against Staphylococcus capitis. The Sawawasabi leaves showed the strongest bactericidal activity with minimal bactericidal concentrations (MBCs) of 156.3mg of dry weight/mL against 2 strains of V. hollisae, V. anguillarum and 2 strains of E. tarda. The roots and stems showed a little weak bactericidal activities with 312-1,250mg of dry weight/mL against them. These results suggest that certain components besides AIT in Sawa-wasabi are affective in killing fish pathogenic bacteria.
Effects of Light, Desiccation and Salinity for the Spore Discharge of Gracilaria verrucosa (Rhodophyta) in Korea
Kim Young Sik ; Choi Han Gil ; Nam Ki Wan ;
Fisheries and aquatic sciences, volume 4, issue 4, 2001, Pages 257~260
The effects of light, desiccation and salinity on the discharge of spores in Korean agarophyte, Gracilaria verrucosa were studied. Among the examined factors, light after darkness was the most effective for spore discharge. The maximum release of tetraspores was induced at 24 h after the treatment. Desiccation also seems to be conductive to the release of tetraspores. However, its effect, as in treatment of distilled water for salinity, was hardly found in induction of carpospore discharge. This may suggest that spore discharge in this alga is primarily related with photoperiodic rhythm. Also it appears that the amount of light energy received by fertile thalli also significantly affects to the spore release, considering relationship between the amount of the discharged spores and the elapsed time after treatment.
Seasonal Variation in Fatty Acid Composition in Female Pen Shell (Atrina Pectinata)
Baik Sung-Hyun ; Kim Kang-Jeon ; Choo Jong-Jae ; Choe Sun-Nam ; Chung Ee-Yung ; Park Kwan Ha ;
Fisheries and aquatic sciences, volume 4, issue 4, 2001, Pages 261~264
Seasonal variation in fatty acid profiles was examined in the visceral mass and the posterior adductor muscle of the female pen shell, Atrina pectinata. Total percentages of saturated fatty acids were similar between the two organs, and there was not a prominent seasonal change in both tissues. While the percentages of highly unsaturated fatty acids (HUFAs) in the visceral mass were higher than those in the posterior adductor muscle, monounsaturated fatty acids (MUFAs) were high in the posterior adductor muscle. HUFA contents, especially in
, markedly decreased in September in the visceral mass, and this decrease was associated with a corresponding total MUFAs in the same organ. A similar pattern of change in September was noted in the posterior adductor muscle MUFAs and HUFAs. These results indicate that
HUFA changes in the visceral mass and posterior adductor muscle reflect the reproductive stages in pen shell.
Fatty Acids Composition in Viscera and Muscle of the Philippines Pinkgray Goby, Amblvchaeturichthys hexanema
Choi Byeong-Dae ; Kang Seok-Joong ; Jeong Bo-Young ; Mendoza Lonarda S. ;
Fisheries and aquatic sciences, volume 4, issue 4, 2001, Pages 265~267
In this study, lipid classes and fatty acids composition in viscera and muscle of the philippines Pinkgray goby, Amblychaeturichtys hexanema were determined. The viscera contained high levels of total lipid
, while the muscle contained less TL
. TL in the viscera consisted of triacylglycerol
, polar lipid
, and cholesterol
. However, the muscle contained a small amount of TG
and much more PL
. Principal fatty acids composition of PL and NL in the viscera and muscle were 16:0, 16:1n-7, 18:0, 18: 1n-9, 18:1n-7, l8:3n-3, 20:5n-3, 22:5n-3, and 22:6n-3.