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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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International Journal of Industrial Entomology
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Korean Society of Sericultural Science
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Volume 1, Issue 2 - Dec 2000
Volume 1, Issue 1 - Sep 2000
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Genetic Factors Affecting Insecticidal Crystal Protein Synthesis in Bacillus thuringiensis
Park, Hyung-Woo ; Brian A. Federici ;
International Journal of Industrial Entomology, volume 1, issue 1, 2000, Pages 1~7
Early studies of the molecular biology of Bacillus thuringeinsis suggested that genetic manipulation of this species could create combinations of genes more useful than those known to occur in natural isolates. Breakthroughs that made these manipulations possible include the cloning of many genes encoding endotoxins, the development of transformation vectors, and various PCR techniques. This paper reviews several genetic factors such as promoters, a 5'mRNA stabilizing sequence, 3'transcription termination sequences, and helper proteins that have been used to enhance crystal protein synthesis, and shows how these genetic elements can be manipulated with new molecular tools to develop more efficacious strains of B. thuringiensis.
Genetic Homogeneity in the Domestic Silkworm, Bombyx, and Phylogenetic Relationship Between B. mori and the Wild Silkworm, B. mandarina Using Mitochondrial COI Gene Sequences
Kim, Iksoo ; Bae, Jin-Sik ; Sohn, Hung-Dae ; Kang, Phil-Don ; Ryu, Kang-Sun ; Sohn, Bong-Hee ; Jeong, Won-Bok ; Jin, Byung-Rae ;
International Journal of Industrial Entomology, volume 1, issue 1, 2000, Pages 9~17
Genetic variation in the domestic silkworm strains (Bombyx mori) and phylogenetic relationships between domestic silkworms and wild silkworms (B. mandarina) were investigated by using a portion of mitochondrial CGI gene sequences. Ten geographic strains of B. mori we sequenced were identical in the 410 bp-section of mitochondrial COI gene. This sequence was also identical to the homologous sequence of the four Gen-Bank-registered strains, but one strain of B. mori differed a single nucleotide (0.2%) from others. MtDNA homogeneity in the B. mori strains appears to be resulted from fixation into the mast frequent mtDNA type during the course of breeding for new strains, in which an extensive indoor rearing and removal of unwanted individuals were accompanied. In the comparisons between domestic and wild silkworms, some wild silkworms were closely related to domestic silkworms (0.2%-1.2% of divergence), but the others were not (2.7%-3.7% of sequence divergence). This result was also reflected in the phylogenetic analyses, showing two independent phylogenetic groups: one including all B. mandarina sequences and the other including both B. mandarina and B. mori sequences. Thus, domestic silkworms may have been derived from the ancestor of B. mandarina, which belongs to this group, alto-ough more extensive study will provide better understanding on this issue.
Construction of a Novel Baculovirus Autographa californica Nuclear Polyhedrosis Virus Producing the Fluorescent Polyhedra
Je, Yeon-Ho ; Jin, Byung-Rae ; Roh, Jong-Yul ; Chang, Jin-Hee ; Kang, Seok-Kwon ;
International Journal of Industrial Entomology, volume 1, issue 1, 2000, Pages 19~23
A novel recombinant baculovirus Autographa californica nuclear polyhedrosis virus (ACNPV) producing the green fluorescent polyhedra was constructed and characterized. The recombinant virus was stably produced fluorescent polyhedra in the infected cells and the morphology of the polyhedra was nearly similar to that of wild-type AcNPV. For the production of the fluorescent polyhedral the green fluorescent protein (GFP) gene was introduced under the control of polyhedrin gene promoter of AcNPV by translational fusion in the front and back of intact polyhedrin gene. The recombinant baculovirus was named as CXEP, As expected, the 93 kDa fusion protein was expressed in the CXEP-infected cells. Interestingly, however, the cells infected with CXEP also showed a 33 kDa protein band as cells infected with wild-type AcNPV. The results of Southern blot analysis and plaque assay suggested that two types of baculoviruses expressing the GFP fusion protein or only native polyhedrin were formed through homologous recombination between two polyhedrin genes in the same orientation. Thus, this system can be applied for the production of recombinant polyhedra with foreign gene product of diverse interest.
Gravimetrics of Pupal Weight Loss in the Domestic Silkworm, Bombyx mori Linn. (Lepidoptera : Bombycidae)
Kumar, Vineet ; Kariappa, B.K. ; Chaturvedi, H.K. ; Sarkar, A. ; Datta, R.K. ;
International Journal of Industrial Entomology, volume 1, issue 1, 2000, Pages 25~28
A comprehensive study on daily pupal weight loss due to histolysis and histgenesis in Bombyx mori Linn., has been undertaken. The percentag of pupal weight loss in the male pupa is higher than the female, conforming that the female pupa require more energy in the form of less pupal weight loss for ovipositional activities. The regression equation clearly shows higher percentage loss of daily pupal weight in male than female with respect to age and, this is also evident from the slope of the regression line. Moreover, analysis of the coefficient of correlation shows that the weight loss of pupa is directly correlated with the age rather than climatic parameters under which the pupa dwells.
Molecular Cloning and Characterization of Very Late Expression Factor 1 Gene, vlf-1 from Bombyx mori Nuclear Polyhedrosis Virus Kl
Park, Hye-Jin ; Lee, Kwang-Sik ; Cho, Eun-Sook ; Yun, Eun-Young ; Kang, Seok-Woo ; Kim, Keun-Young ; Sohn, Hung-Dae ; Jin, Byung-Rae ;
International Journal of Industrial Entomology, volume 1, issue 1, 2000, Pages 29~33
We cloned and characterized a very late expression factor 1 gene, vlf-1, which regulates the level of very late gene transcripts, from Bombyx mori nuclear polyhedrosis virus (BmNPV) K1 strain. The 1,140 bp vlf-1 has an open reading frame of 379 amino acid and a MW of 44 kDa. The vlf-1 nucleotide sequence of BmNPV-Kl showed high homology with Autographa californica nuclear polyhedrosis virus and BmNPV T3 strain so far known, and its deduced amino acid residues were identical to those of BmNPV T3. The location of vlf-1 in the BmNPV-Kl genome was confirmed by Southern blot analysis and its expression patterns at the transcriptional level were confirmed by Northern hybridization analysis.
Effect of Methyl Testosterone on the Economic Parameters of the Silkworm, Bombyx mori L.
Goudar, K.S. ; Kaliwal, B.B. ;
International Journal of Industrial Entomology, volume 1, issue 1, 2000, Pages 35~40
Effect of Hydrocortisone on the Economic Parameters of the Domestic Silkworm, Bombyx mori L.
Goudar, K.S. ; Kaliwal, B.B. ;
International Journal of Industrial Entomology, volume 1, issue 1, 2000, Pages 41~45
The effect of topical application with hydrocortisone on economic parameters was analysed following treatment of last larval stadium. The treated larvae showed a significant increase in larval weight at higher concentrations along with other enhanced larval, cocoon and adult parameters. This suggests that hydrocortisone can be used effectively for commercial silkworm rearing. The larvas period was significantly increased in all the treated groups with increased female cocoon weights, its shell weights and male cocoon shell weight at 20
/ml treated group. Filament length, weight and denier were increased significantly in all the treated groups. Moth emergence percentage, length of the ovariole, eggs per ovariole, fecundity and rind hatching percentage were unaffected when compared with that of the carrier control group. This suggests that hydrocortisone in addition to affecting larval growths also affect silk crop.
Effect of Potassium Nitrate Supplementation on Economic Parameters of the Silkworm Bombyx mori L.
Goudar, K.S. ; Kaliwal, B.B. ;
International Journal of Industrial Entomology, volume 1, issue 1, 2000, Pages 47~52
The effect of supplementation with potassium nitrate on economic parameters was analysed following treatment of last larval stadium. The treated larvae showed a significant increase in larval weight at 500
/ml and silk gland weight in all the treated groups along with other enhanced larval, cocoon and adult parameters. The larval period was significantly decreased in all the treated groups with increased female and male cocoon weights its shell weights filament lengths at 500
/ml. The denier was significantly decreased in all the groups. The filament weight moth emergence percentaged length of the ovariole were unaffected. However, eggs per ovariole, fecundity and hatching percentage significantly decreased compared with the corresponding parameters of the carrier control.
Expression of the Gene Encoding Firefly Luciferase Using Bombyx mori Nucleopolyhedrovirus Vector
Woo, Soo-Dong ; Cho, Kook-Ho ; Jin, Byung-Rae ; Boo, Kyung-Saeng ; Kang, Seok-Kwon ;
International Journal of Industrial Entomology, volume 1, issue 1, 2000, Pages 53~58
A cDNA encoding the luciferase of firefly Luciola lateralis was cloned downstream from the polyhedrin gene promoter of Bombyx mori nucleopolyhedrovirus and expressed in B. mori cells (BmN-4). The coding soquence for luciferase was inserted into pBmKSK2 rectors) which was reconstructed from the polyhedrin-based transfer vector pBmKSKl by modifying cloning sites. Recombinant virus, BmK2-LUCDF, containing the luciferase gene was selected and purified in BmN-4 cells. The emission of luminescence by luciferase was only detected in BmK2-LUCDF-infected cell extracts. This result indicates that the cloned new luciferase gene of firefly L. lateralis can be expressed efficiently in baculovirus expression system and used as a useful reporter gene.
Studies on PVA/Chitosan/Fibroin Blend Sponge Sheets: Preparation and Wound Healing Effects in Rats
Yeo, Joo-Hong ; Lee, Kwang-Gill ; Lee, Heui-Sam ; Lee, Yong-Woo ; Kim, Sun-Yeou ;
International Journal of Industrial Entomology, volume 1, issue 1, 2000, Pages 59~64
The preparation of PVA/Chitosan/Fibroin (PCF)-blended sponge sheets and wound healing effects of these sheets in rats were investigated. We excised the skin off rat, including the dermis, approximately 2
2 cm in size. The wound was coveted with PCF-blended spongy sheets. The spongy sheets absorbed the exudate, and gained flexibility and softness. Histopathological inspection of the wound 12 days later showed the increase of a vascular ingrowth and the absence of inflammatory cells. Regeneration of the skin around the wound was faster than that of the control. We also tested wound healing effects of PVA, Chitosan and Fibroini alone of in various combinations. Wound healing was accelerated in the order of PVA/chitosan/Fibroin (PCF)-blended sponge>Chitosan/Fibroin (CF)-blended sponge
Fibroin (F) sponge>PVA/Chitosan-blended sponge (PC)>Chitosan (C) sponge.
Studies on the Seasonal Incidence of the Whitefly (Dialeuropora decempuncta Quaintance and Baker) Causing Leaf Curl on Mulberry in Relation to Abiotic Factors
Bandyopadhyay, U.K. ; Sahu, P.K. ; Raina, S.K. ; Santhakumar, M.V. ; Chakraborty, N. ; Sen, S.K. ;
International Journal of Industrial Entomology, volume 1, issue 1, 2000, Pages 65~71
A study was conducted to examine the relationship between abiotic factors and the population of white-fly (Dialeuropora decempuncta) in mulberry field. The study reveals that relationship between abiotic factors and the whitefly population is very much existent like other pests in other agricultural crops. Duration and time of distinct occurrence of whitefly in mulberry is influenced by the abiotic conditions of field. Abiotic parameters of previous month are more important in influencing the intensity of the pest than the current abiotic factors. Not all the abiotic factors are equally important but factors like minimum temperature, fluctuation in temperature during the days minimum relative humidity, fluctuation in relative humidity and rainfall are the major important lactors in influencing the intensity of the pest under consideration.
Biochemical Properties of Acetylcholinesterase from the Larval Head of Bombyx mori
Lee, Hwa-Jun ; Lee, Heui-Sam ; Lee, Pyeong-Jae ; Cho, Il-Je ; Lee, Sang-Mong ; Moon, Jae-Yu ;
International Journal of Industrial Entomology, volume 1, issue 1, 2000, Pages 73~78
We investigated some biochemical properties of acetylcholinesterase (AChE) in the Bombyx mori larval head. 1% Triton X-100 (v/v) was suitable for extracting AChE from the silkworm larval head but 1 M NaCl was not suitable. PAGE analysis showed a single band of AChE that was detected by histochemical staining using acetylthiocholine as a substrate. AChE was also partially purified with Sepharose 6B and DEAE-cellulose column. Finally, the specific activity of partially purified enzyme solution was 7.6. The study on inhibitor specificity indicated that the enzyme under study was a true cholinesterase (ChE) or AChE. AChE activity was maximum at the substrate concentration of
M and the excess substrate inhibited the AChE activity. The optimal pH and temperature were pH 7.0-9.0 and 30-35