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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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International Journal of Industrial Entomology
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Journal DOI :
Korean Society of Sericultural Science
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Volume & Issues
Volume 11, Issue 2 - Dec 2005
Volume 11, Issue 1 - Sep 2005
Volume 10, Issue 2 - Jun 2005
Volume 10, Issue 1 - Mar 2005
Selecting the target year
Inter Simple Sequence Repeat (ISSR) Polymorphism and Its Application in Mulberry Genome Analysis
Vijayan Kunjupillai ;
International Journal of Industrial Entomology, volume 10, issue 2, 2005, Pages 79~86
Molecular markers have increasingly been used in plant genetic analysis, due to their obvious advantages over conventional phenotypic markers, as they are highly polymorphic, more in number, stable across different developmental stages, neutral to selection and least influenced by environmental factors. Among the PCR based marker techniques, ISSR is one of the simplest and widely used techniques, which involves amplification of DNA segment present at an amplifiable distance in between two identical microsatellite repeat regions oriented in opposite direction. Though ISSR markers are dominant like RAPD, they are more stable and reproducible. Because of these properties ISSR markers have recently been found using extensively for finger printing, pohylogenetic analysis, population structure analysis, varietal/line identification, genetic mapping, marker-assisted selection, etc. In mulberry (Morus spp.), ISSR markers were used for analyzing phylogenetic relationship among cultivated varieties, between tropical and temperate mulberry, for solving the vexed problem of identifying taxonomic positions of genotypes, for identifying markers associated with leaf yield attributing characters. As ISSR markers are one of the cheapest and easiest marker systems with high efficiency in generating polymorphism among closely related varieties, they would play a major role in mulberry genome analysis in the future.
Mating Behaviour in Mulberry Silkworm, Bombyx mori (L.)
Saheb N. M. Biram ; Singh Tribhuwan ; Kalappa H. K. ; Saratchandra B. ;
International Journal of Industrial Entomology, volume 10, issue 2, 2005, Pages 87~94
Mating is an essential behavioural social event in the life cycle of silkworm, Bombyx mori (L.) for the perpetuation of population. A number of intrinsic and extrinsic factors and events of significant importance are involved in successful mating and egg deposition by an adult silk moth which besides biochemical, physiological and environmental factors also includes attraction of reproductively competent male and female moth for mating, duration and frequency of mating, age of moth at the time of mating, reuse of male moth in the production of eggs etc. An attempt has been made in this review article to elucidate briefly the behaviour of male towards female moth after eclosion, impact of duration and frequency of mating on egg deposition and oviposition, reuse of mated male moth in the production of quality and quantity eggs etc. in the silk-worm, B. mori and its significance in silkworm seed production.
Genetic Improvement of Some Traits in Four Strains of Silkworm, Bombyx mori L.
Moghaddam S. H. Hosseini ; Jomeh K. N. Emam ; Mirhosseini S. Z. ; Gholamy M. R. ;
International Journal of Industrial Entomology, volume 10, issue 2, 2005, Pages 95~99
A breeding plan was carried out on four commercial strains of silkworm (Bombyx mori L.) 101, 102, 103 and 104 to improve some important traits. Genetic gain or response to selection
, heritability of cocoon shell weight (CSW) and specific combining ability effects were estimated to determine the strains that can be improved. Strain 101 had lowest heritabitity,
and viability. Strain 102 was acceptable in selection response but its viability was low. Therefore these two strains were not suitable for more selection. As a result, only lines 103 and 104 were chosen for further improvement. Intra population selection based on independent culling level method practiced from third to sixth generation for both productive and viability traits simultaneously. While CSW and CW had increasingly enhanced during primary generations, they went slightly up after third generation. According to negative genetic correlation, viability decreased during primary generations, but after third generation that paid attention to balanced development of both productive and viability traits, viability increased so that the pupation rate reached to
in 103 and
in 104 for last generation
cDNA Cloning and Developmental Expression of Hemolin in Bombyx mandarina
Kang Min Uk ; Kim Kyung-A ; Lee Jin Sung ; Kim Nam Soon ; Kang Seok-Woo ; Nho Si-kab ;
International Journal of Industrial Entomology, volume 10, issue 2, 2005, Pages 101~106
In this study, we describe the Bombyx mandarina hemolin cDNA. A sequence analysis of cDNA revealed a single open reading frame (ORF) of 1233 nucleotides. The deduced 410 amino acid sequence of B. mandarina hemolin contains 4 imunoglobulin (Ig) C-2 type domains. B. mandarina hemolin cDNA showed the highest sequence homology to known those of B. mori. The developmental profile in terms of expression level of hemolin mRNA was determined in the absence of a bacterial challenge. Hemolin mRNA was detected only in mid-gut, but not in hemocytes, fat body, testis, and silkglands. Hemolin mRNA in mid-gut was not detected until the spinning stage of the last instar larva, however, lit dramatically increased at the beginning of spinning and gradually decreased until pupal stage.
Synergetic Effect of Minerals Mixture of Potassium Bromide and Nickel Sulphate on the Economic Traits of
Crossbreed Races of the Silkworm, Bombyx mori L.
Kochi S. C. ; Kaliwal B. B. ;
International Journal of Industrial Entomology, volume 10, issue 2, 2005, Pages 107~117
Oral supplementation of minerals mixture of potassium bromide and nickel sulphate on
crossbreed races with different concentrations (70, 110 and
) were fed to the fifth instar larvae. Two of the four normal feeds per day was substituted with treated leaves fed to silkworm at fifth instar larvae. The controls were fed with the leaves sprayed with distilled water and normal leaves. The
race treated larvae showed a significant increase in silkgland weight, cocooning percentage, female cocoon weight, male cocoon weight, male cocoon shell weight, male cocoon shell ratio, filament length, filament weight, denier, fecundity, number of eggs per ovariole and length of the ovariole when compared with those of the corresponding parameters of the carrier control, whereas in
crossbreed races showed a significant increase in larva1 weight and other parameters similar to that of
race. The results suggested that the silkgland showed good response to minerals mixture of potassium bromide and nickel sulphate only in
race when compared with those of other two races of the silkworm, B. mori.
Isolation and Characterization of Benomyl-Resistant Mutants in an Entomopathogenic Fungus, Metarhizium anisopliae
Kim Soon Kee ; Shim Hee Jin ; Roh Jong Yul ; Jin Byung Rae ; Boo Kyung Saeng ; Je Yeon Ho ;
International Journal of Industrial Entomology, volume 10, issue 2, 2005, Pages 119~123
Benomyl-resistant mutants of entomopathogenic fungus, Metarhizium anisopliae were isolated and their physiological characteristics were investigated. These militants were obtained spontaneously or by UV irradiation in benomyl-treated media. Four spontaneous (S-2, S-11, S-18, S-19) and four UV-induced (UV-4, UV-5, UV-19, UV-24) mutants, which grow stably and normally were selected. No significant differences in conidia or hyphal shape, conidia viability, mycelial biomass, or virulent to the diamondback moth were observed between the wild type and their mutants. But differently from the mycelial growth of other benomyl-resistant mutants which was slower than that of the wild type on a modified Czapek-Dox, SDAY,
skim milk medium, that in the spontaneous mutants, S-18 and S-19, did not show any difference from the wild type. Especially, S-18 and S-19 grew well at benomyl concentrations up to 50 times or higher than that which inhibits wild type proliferation. These results suggested that S-18 and S-19 could potentially be used with the fungicide, benomyl.
Cloning and Characterization of Ribosome-associated Membrane Protein 4 (RAMP4) gene in silkworm Bombyx mori
Yao Qin ; Hu Zhigang ; Xu Jiaping ; Chen Keping ;
International Journal of Industrial Entomology, volume 10, issue 2, 2005, Pages 125~129
Ribosome-associated membrane protein 4 (RAMP4) is a membrane protein that exposes its N-terminal hydrophilic portion on the cytoplasmic side and spans the membrane close to the C-terminal end. RAMP4 has previously been reported to belong to the set of proteins that remains associated with membrane-bound ribosomes, and controls the glycosylation of major histocompatbility complex class II-associated invariant chain. RAMP4 also may be relative to the stabilization of membrane proteins in response to stress, with other components of translocon, and molecular chaperons in ER. Application of 5'-RACE technique with specially designed primer, we cloned a 715 bp cDNA fragment which contains a 195 bp ORF, termed RAMP4. The deduced protein has 64 amino acid residues and contains a putative transmembrane-spanning domain at the COOH terminus.
Identification of a pr 1-like Gene of Entomopathogenic Fungus, Beauveria bassiana F-101 Isolated from Thecodiplosis japonensis
Shin Sang Chul ; Roh Jong Yul ; Shim Hee Jin ; Kim Soon Kee ; Kim Chul Su ; Park Il Kwon ; Jeon Mun Jang ; Je Yeon Ho ;
International Journal of Industrial Entomology, volume 10, issue 2, 2005, Pages 131~136
Beauveria bassiana F-101, which has high toxicity toward Acantholyda parki as well as Thecodiplosis japonensis, was an isolate to develop an alternative control system against the major forest pests. Up to now, in B. bassiana, only one pr1 gene has been isolated and characterized. Therefore, we here reported the identification of a pr1-like gene, which would be a factor of toxicity from B. bassiana F-101. The oligonucleotides for the amplification of the pr1-like gene, were chosen based on the conserved regions of the subtilisin family enzymes, pr1 genes of B. bassiana and Metarhizium anisopliae, and proteinase K of Tritirachium album. The cloned PCR fragment had 1111 bp including 52 bp intron. The deduced Pr1-like peptide showed a low identity with Pr1s of entomopathogenic fungi such as B. bassiana Pr1 (BbPr1) and M. anisopliae Pr1 (MaPr1) as well as the proteinase K of T. album (TaPrK). Instead, the deduced peptide had a substantially high amino acid sequence identity $(>65\%)$ with the serine proteases of Magnaporthe grisea (MgSPM1) and Podospora anserina (PaPspA). These results, therefore, appear to suggest that the putative Pr1-like peptide of B. bassiana F-101 belongs to the subtilisin-like serine protease family and may be a novel gene.
Acquiring Vitellogenic Competence in the Rice Pest Nilaparvata lugens Stal: Effects of a Juvenile Hormone Analogue, Hydroprene
Pradeep A. R. ; Nair V. S. K. ;
International Journal of Industrial Entomology, volume 10, issue 2, 2005, Pages 137~141
Though many insecticides are commercially available, development of resistance, pest resurgence and effects on non-target organisms led to the search for alternate insect pest management (IPM) strategy based on larval growth and reproductive fitness. Reproductive potential of insects depends on its acquiring of vitellogenic competence which is under hormonal control. Exogenous application of analogues of JR (JHAs) and ecdysterone could derail normal development and reproduction in insects by manipulating an array of physiological processes. In the rice pest, brown planthopper, Nilaparvata lugens, JHA, hydroprene induced metathetely from the fifth (final) instar nymphs in an age-dependent manner. Day 0 of the final instar showed highest sensitivity to induce this abnormal development. Adults emerged from treated day 3 nymphs looked normal. Both the morphotypes were reproductively incompetent and showed partial to complete sterility. Pre-adult exposure of the ovarian tissue to hydroprene suppressed mitotic division of germinal cells and induced abnormalities in the later s1ages of growth and differentiation of ovary in N. lugens. More over the nymphal exposure to hydroprene inhibited patency changes of follicular epithelium and affected competence of the follicles for yolk sequestration. In the absence of ovarian growth and oocyte differentiation, germarium found disintegrated, trophic core regressed and terminal oocytes resorbed. Hydroprene exposure to newly ecdysed brachypterous females did not affect ovarian development and egg production. Proper larval-adult transition appeared as a. prerequisite for vitellogenic competence in N. lugens for which the ovarian tissues must be exposed to ecdysterone in the internal milieu devoid of JH.
A Report on the Impact of a Microsporidian Parasite on Lamerin Breed of the Silkworm Bombyx mori L.
Bhat Shabir Ahmad ; Nataraju B. ;
International Journal of Industrial Entomology, volume 10, issue 2, 2005, Pages 143~145
Lamerin breed of the silkworm, Bombyx mori L. for Northeastern India hosts a vertically transmitted microsporidian parasite for generations, which does not harm significantly the cocoon production. The transversally infected progenies do not exhibit marked external sign or symptom. The microspordian causes inapparent infection and over
of the infected progeny survives and spin cocoons. There is possibility of co-existence between the breed and the associated micosporidian parasite. To evaluate the impact of the microsporidian on breed the present study was conducted in respect of tranovarial transmitted (observed as T1), secondarily infected (observed as T2) and healthy silkworm (observed as T3). The larval and pupal mortality was
in T1 and
in T2 batch, while in case of T3 batch there was no mortality. Significant changes were also observed in single cocoon weight, single shell weight, denier, reelibility, raw silk recovery
and neetness. There is no significant impact of the infection on the fecundity and hatchability. The hatchability of the eggs laid by healthy or infected moths are equall as much as control but the progeny had the infection transmitted from the parent.
Development of Fine Denier Silkworm Hybrid
of Bombyx mori L. for Superior Quality Silk
Kalpana G. V. ; Kumar N. Suresh ; Basavaraja H. K. ; Reddy N. Mal ; Palit A. K. ;
International Journal of Industrial Entomology, volume 10, issue 2, 2005, Pages 147~151
Serious attempt for the improvement of quality parameters in silkworm hybrids came rather very late in India. Realising the need for productive breeds to the field and reorientation in breeding methodology, the silkworm breeders of CSRTI, Mysore were able to develop silkworm breeds with fine denier namely, JPN7, CSR48, B63 and B65 of specific industrial requirement through directional selection. As the success of silkworm breeds mainly depends on their combining ability, the developed breeds were subjected for hybrid evaluation. Out of 42 bivoltine hybrids studied, one hybrid,
was identified for its superiority over the existing bivoltine hybrids namely
for majority of qualitative traits. Post cocoon testing of large quantity of cocoons of
resulted in the production of high quality '3A'grade silk. It is for the first time in the Indian Sericulture industry that high quality silk with longer filament length of 1474 m, denier of 2.47, standard size deviation of 0.983 d, maximum size deviation of 1.322, neatness of 96 p, reelability of
, tenacity of 3.87 g/d, elongation of
and cohesion of 110 strokes was produced. The superiority of fine denier silkworm breeds and their hybrids are discussed.
The Combining Ability Analysis and Heterosis for some Quanitatives Traits in the Silkworm (Bombyx mori L.)
Moghaddam S. H. Hosseini ; Etebari K. ;
International Journal of Industrial Entomology, volume 10, issue 2, 2005, Pages 153~157
Recently two breeding programs were carried out for isolation of new parental inbred lines in Iran. This study was undertaken in order to estimate the combining ability effects and heterosis of these lines. For this purpose an
diallel cross analysis including eight inbred lines of silkworm with four lines from each program were studied for their five quantitative traits. The results indicated that reciprocal effects appeared insignificant for most of traits. Japanese lines of 111 and 113 were best combiners for shell weight, cocoon weight and shell percentage traits. With respect to pupation rate, Chinese line 110 was the best and according to general combining ability (GCA) and specific combining ability (SCA) variances, it is superior in specific combinations. Hybrid
showed better characters for both productive and viability traits. Hybrids with high heterosis had high SCA too.