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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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International Journal of Industrial Entomology
Journal Basic Information
Journal DOI :
Korean Society of Sericultural Science
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Volume & Issues
Volume 11, Issue 2 - Dec 2005
Volume 11, Issue 1 - Sep 2005
Volume 10, Issue 2 - Jun 2005
Volume 10, Issue 1 - Mar 2005
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Mode of Transmission of a Newly Discovered Microsporidian and Its Effect on Fecundity and Hatching in Silkworm, Bombyx mori L.
Bhat Shabir Ahmad ; Nataraju B. ;
International Journal of Industrial Entomology, volume 11, issue 2, 2005, Pages 81~86
The mode of transmission, effect on fecundity, hatching and tissues specificity of a microsporidian
recovered from Lamerin breed of the silkworm Bombyx mori L. was studied and compared with standard strain Nosema bombycis. Peroral inoculation of
or N. bombycis to zeroday of
instar larvae of silkworm was the most suitable method for producing information on development of stage specific mortality, pupation and obtaining infected adults for transovarial transmission studies. It was observed that pupal mortality, the percentage of moths emerged and the percentage of moths infected were significantly high in N. bombycis infected batches as compared
in all the three tested breeds of the silkworm. However no significant difference was observed in larval mortality. The fecundity and hatchability was not affected significantly in
infected adults, however significant reduction in egg production, fecundity, hatchability and increased egg retention was observed in mother moths infected with N. bombycis. The
is transmitted both horizontally and vertically at lower rate due to its low rate of proliferation. The trans ovarial transmission of
progeny generation through eggs averaged only
whereas N. bombycis was transmitted at
had low oral infectivity and low transovarial transmission in silkworm B. mori.
Isolation and Characterization of Plutella xylostella Granulovirus Isolated in Korea
Wang Yong ; Choi Jae Young ; Kang Joong Nam ; Kim Yang-Su ; Choi Heekyu ; Roh Jong Yul ; Li Ming Shun ; Jin Byung Rae ; Im Dae Joon ; Je Yeon Ho ;
International Journal of Industrial Entomology, volume 11, issue 2, 2005, Pages 87~91
We have first isolated and characterized a Plutella xylostella granulovirus (
) from dead larvae of P. xylostella in Korea. The granule of
was ovoidal shape with an approximate measure of
, and each granule contained one single rod-shape virion with a mean size of
. The major granule protein, granulin, had a molecular weight of approximately 29kDa. Whereas the nucleotide sequence of the granulin gene was identical to that of previously reported
, nucleotide sequences of two of three putative p10 genes were slightly different from those of reported
. These results suggested that the
isolated in this study was a novel isolate containing different genomic information.
Effect of Extended Egg Preservation Schedule in Conservation of Mutant Silkworm (Bombyx mori L.) Genetic Stocks in Gene Bank
Muthulakshmi M. ; Mohan B. ; Balachandran N. ; Sinha R. K. ; Thangavelu K. ;
International Journal of Industrial Entomology, volume 11, issue 2, 2005, Pages 93~98
Studies on extended egg preservation schedule from 120 days to 180 days was taken up with 20 germplasm accessions of mutant silkworm genetic stocks of Bombyx mori L. Statistical analyses of the data collected over three trials revealed no significant changes both in the qualitative and quantitative traits of the genetic stocks between treatment (6 months egg preservation) and control (4 months egg preservation), except for fifth instar larval duration in TMS-61, TMS-62, TMS64, TMS-31 and TMS-34 shell weight in TMS-62, TMS-64 and TMS-66. Thus, the results indicate that extended schedule of 6 months egg preservation can safely be adopted, which will reduce the cost of conservation and minimize the genetic erosion owing to reduced crop cycle.
Oviposition and Colony Development of the Bumblebees, Bombus ignitus and B. terrestris depending on Different Pollen
Yoon, Hyung-Joo ; Kim, Sam-Eun ; Lee, Kyeong-Yong ; Lee, Sang-Beom ; Park, In-Gyun ;
International Journal of Industrial Entomology, volume 11, issue 2, 2005, Pages 99~105
We investigated oviposition and colony development of Bombus ignitus and B. terrestris depending on different pollen. In five kinds of pollen blend, the colony development in fresh-freezing pollen blend produced in Korea (Korea-FFP) was the best performance, although egg-laying characteristics is lower than that of pollen blend for oviposition imported from Korppert company (Kopport-FOP). The Kopport-FOP proved that it was suitable to use for oviposition of bumblebees. The Korea-FFDP, freezing dried-fresh pollen blend produced in Korea, is lower rather than the Korea-FDP although it is similar to the KopportFOP in colony development. It is not efficient to use commercial pollen for bumblebee because it is expensive in cost. The dried pollen blend for honeybee feeding imported from China (China-DP) was not suitable for rearing of bumblebee because it did not form colony although the worker emerged. In types of pollen, the oviposition and colony development of B. ignitus were not affected by the fresh-freezing pollen and dried-freezing pollen. This result also indicated that dried pollen, dried in the shade for 5 - 6 days, is possible to use as commercial pollen for bumblebee reproduction.
Seasonal Impact of Microsporidian Infection on the Reproductive Potential of Silkworm, Bombyx mori L. (Lepidoptera: Bombycidae)
Mohanan N. Madana ; Krishnan N. ; Mitra P. ; Das N. K. ; Saratchandra B. ; Haldar D. P. ;
International Journal of Industrial Entomology, volume 11, issue 2, 2005, Pages 107~111
Impact of microsporidian infection and season on reproductive potential of Bombyx mori L. was investigated in the laboratory. Microsporidian infection significantly (P<0.01) reduced fecundity and hatching and increased sterility and mortality of eggs. Among the microsporidia, Nosema sp. 2 infected silkworm produced eggs with least fecundity and hatching percent as well as highest dead and sterile eggs followed by Nosema sp. 1 and N. bombycis. Microsporidia, in general, significantly reduced fecundity and hatching percent of eggs and increased number of dead and sterile eggs in all the three seasons except N. bombycis in July - August (S3) and Nosema sp. 2 in January February (S1). Since, seed production is the anchor sheet of mulberry sericulture, coefficient of egg lying is considered as an important aspect and the industry quite often facing shortage of disease free layings. The present study indicates that B. mori is more susceptible to microsporidia during S2 followed by S3 and S1 and Nosema sp2. is most virulent followed by Nosema spl. and N. bombycis.
A Chymotrypsin Gene Homologue from the Mulberry Longicorn Beetle, Apriona germari: cDNA Sequence Characterization and mRNA Expression Pattern
Gui Zong Zheng ; Lee Kwang Sik ; Yoon Hyung Joo ; Kim Iksoo ; Sohn Hung Dae ; Jin Byung Rae ;
International Journal of Industrial Entomology, volume 11, issue 2, 2005, Pages 113~117
A chymotrpsin gene homologue was cloned from the mulberry longicorn beetle, Apriona germari. The A. germari chymotrypsin cDNA contains an ORF of 950 nucleotides capable of encoding a 283 amino acid polypeptide with a predicted molecular mass of 29151 Da and pI of 9.38. The A. germari chymotrypsin has conserved six cysteine residues and active triad formed by His, Asp and Ser. The deduced amino acid sequence of the A. germari chymotrypsin cDNA was closest in structure to the Anthonomus grandis chymotrypsin. Northern blot analysis revealed that A. germari chymotrypsin showed the midgut-specific expression.
Heterobeltiotic Genetic Interaction between Congenic and Syngenic Breeds of Silkworm, Bombyx mori L.
Verma A. K. ; Chattopadhyay G. K. ; Sengupta M. ; Das S. K. ; Sarkar A. K. ;
International Journal of Industrial Entomology, volume 11, issue 2, 2005, Pages 119~124
To determine the level of heterosis, higher cocoon shell weight multivoltine congenic lines (Con. L) and bivoltine syngenic lines (Syn. L) of silkworm were used for crosses. First filial generations
expressed heterobeltiotic genetic interaction at significant magnitude (p < 0.01) for single cocoon shell weight (SCSW). The other linked characters viz., single cocoon weight (SCW) and yield by weight per 10, 000 larvae were also significantly higher (p < 0.01) than the better parental lines. All the hybrids showed significant improvement for these aforesaid characters over standard heterosis (Standard check). The reeling parameters viz., filament length, raw silk, neatness, cohesionstrokes etc, also showed improvement among the hybrids than check in congenial environment. Overall results suggested that the cross between congenic and syngenic lines provide better heterosis with good quality silk than conventional hybrids and may be used for commercial exploitation.
Construction of a High-efficiency Shuttle Vector Containing the Minimal Replication Origin of Bacillus thuringiensis
Kang Joong Nam ; Kim Yang-Su ; Wang Yong ; Choi Heekyu ; Li Ming Shun ; Shin Sang Chul ; Jin Byung Rae ; Roh Jong Yul ; Choi Jae Young ; Je Yeon Ho ;
International Journal of Industrial Entomology, volume 11, issue 2, 2005, Pages 125~127
In order to improve the transformation efficiency of the Bacillus thuringiensis (Bt)-Escherichia coli (E. coli) shuttle vector, pHT3101, we intended to minimize replication origin of Bt in pHT3101. For this, two modified shuttle vectors, pHT1K and pHT261, in which 2.9 kb of replication origin of Bt were shortened to 1 kb and 261 bp, respectively as previously reported. Whereas the pHT1K could efficiently transform Bt into the antibiotic resistant, no transformants were obtained with pHT261. Furthermore, pHT1K showed higher transformation efficiency compared to that of parent vector, pHT3101. Therefore, pHT1K might be a very useful Bt-E. coli shuttle vector carrying minimal replication origin of Bt.
Influence of Weather Factors on the Incidence of the Mulberry Aleyrodid, Dialeuropora decempuncta (Quaintance and Baker) and Their Relation to Yield Loss
Bandyopadhyay U. K. ; Santhakumar M. V. ; Sahu P. K. ; Saratchandra B. ;
International Journal of Industrial Entomology, volume 11, issue 2, 2005, Pages 129~133
The seasonal occurrence and influence of abiotic factors viz., maximum and minimum temperature, maximum and minimum humidity and rainfall on population fluctuation of aleyrodid, Dialeuropora decempuncta on a evolved mulberry (Morus alba L.) variety known to be susceptible to aleyrodid infestation was assessed during the period from 1999 - 2001 in twenty-five villages under nine blocks of Malda district of West Bengal. The results indicate that the aleyrodid population is practically very low or absent during January to June and thereafter increases gradually. The increase in population of various stages of aleyrodid is significantly correlated with increase in previous 7 days of average maximum relative humidity.
PCR-Based Detection of Densovirus Infection in Silkworm (Bombyx mori L.)
Hou Chengxiang ; Li Muwang ; Gui Zhongzheng ; Xu Anying ; Guo Xijie ;
International Journal of Industrial Entomology, volume 11, issue 2, 2005, Pages 135~138
Two pairs of DNA primers were designed for the detection of the Zhenjiang (China) strain of Bombyx mori densonucleosis virus (BmDNV-Z). These primers were designed from the nucleotide sequence of major structural protein gene (putative VD1-ORF2). PCR amplification was attempted from different issues (including silk gland, blood, skin and midgut) and feces of the silkworm which infected wit BmDNV-Z were amplified by PCR. Both of the primers gave expected size of in the DNA bands from midgut and feces, but not in the DNA of silk gland, blood and skin. The two bands were sequenced, and their sequence were same as the sequence designed for. BmDNV-Z could be successfully detected in single silkworm after it was infected for 12 hrs, and could not be detected before 9 hrs after infected.
Implications of High Temperature and Low Humidity on the Hatching in the Silkworm, Bombyx mori L. Under LD 12 : 12 Condition
Reddy P. Lakshminarayana ; Naik S. Sankar ; Reddy N. Sivarami ;
International Journal of Industrial Entomology, volume 11, issue 2, 2005, Pages 139~143
The implications of temperature (25, 30 and
) and relative humidity (RH; 60, 70 and
) on the hatching rhythmicity and hatching parameters (percentage and duration) were studied in the silkworm, Bombyx mori L. under natural photoperiod (LD 12 : 12). Disease free layings (DFLs) of two pure silkworm breeds, Pure Mysore (PM, a multivoltine breed) and
(a bivoltine breed), and their hybrid,
were introduced into the experimental conditions on the
day of oviposition till completion of hatching. The hatching rhythm was predominantly diurnal under all temperature and humidity conditions, with peaks just after 'lights-on' phase (6 hrs). Extreme temperature and humidity conditions did not alter the hatching rhythmicity, but prolonged the hatching durations, extending it to the next day, coupled with reduced hatching percentage in PM and
, on the other hand, hatching did not extend to the next day. Hatching percentage in this breed, however, reduced below the economic level under high temperature and low humidity conditions. The high temperature and low humidity together, though did not alter the rhythmicity, seems to exert synergetic effect on the hatching percentage and its duration in the silkworm, B. mori.