Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
International Journal of Industrial Entomology
Journal Basic Information
Journal DOI :
Korean Society of Sericultural Science
Editor in Chief :
Volume & Issues
Volume 15, Issue 2 - Dec 2007
Volume 15, Issue 1 - Sep 2007
Volume 14, Issue 2 - Jun 2007
Volume 14, Issue 1 - Mar 2007
Selecting the target year
Evaluation and Selection of Potential Parents Based on Selection Indices and Isozyme Variability in Silkworm, Bombyx mori, L.
Moorthy S.M. ; Das S.K. ; Rao, P.R.T. ; Urs S. Rao, ; Sarkar A. ;
International Journal of Industrial Entomology, volume 14, issue 1, 2007, Pages 1~7
In order to find out the appropriate parents for the breeding programme, twelve bivoltine and three multivoltine silkworm breeds were evaluated on the basis of multivariate selection index and isozyme analysis. Of which, four [CSR2, D6 (P), SK3, SK4] bivoltine and two multivoltine (Nistari, Cambodge) breeds were selected and breeding initiated to develop higher survival bivoltine silkworm breed suitable for tropical conditions. Among two isozyme (Esterase and acid phosphatase) analyzed, only esterase exhibited polymorphism among the bivoltine breeds. No polymorphism was observed among multivoltine in respect of esterase as well as acid phosphatase.
Insecticidal Efficacy of Porteresia coarctata (Roxb.) on Bio-chemical Alteration of Spodoptera litura (Fab.)
Bhattacharyya Atanu ; Goswami Arunava ; Dihidar Shankar ; Adhikari Sujit ; Banerjee Sudip Kumar ; Talapatra Soumendra Nath ; Barik Bikas Ranjan ; Bhattacharya Subir Kumar ; Chandra Asit Kumar ;
International Journal of Industrial Entomology, volume 14, issue 1, 2007, Pages 9~13
Hexane extract of Porteresia coarctata (Roxb.) exhibits a toxic effect on the tissues of Spodoptera litura (F) while fed at the dose of 1000 and 2000 ppm thoroughly mixing with castor leaves (Ricinus communis L) after dissolving in DMSO at late fourth instar whereas only DMSO treated castor leaves were fed to control group. The larvae were put to rear at
R.H. under 12 L + 12 D photoperiodic regime. In test group insects substantial reduction of protein and DNA content was marked in fat body and midgut tissues compared to DMSO treated control group. The significant biochemical alterations in the midgut tissues and fat body of test group insects indicate the insecticidal property of the said plant extract that could be tested in facilitating the phenomenal stride in Integrated Pest Management.
Influence of Different Environmental Conditions on Cocoon Parameters and Their Effects on Reeling Performance of Bivoltine Hybrids of Silkworm, Bombyx mori. L.
Gowda B. Nanje ; Reddy N. Mal ;
International Journal of Industrial Entomology, volume 14, issue 1, 2007, Pages 15~21
Three newly authorized bivoltine silkworm hybrids namely,
(productive single hybrid),
(productive double hybrid) and
(robust single hybrid) were chosen for the present study. These hybrids were subjected to different temperature and humidity treatments i.e.,
, with combinations of low relative humidity (RH
) and high RH (
) at different stages during rearing and spinning of silkworm larvae. The larvae of after 3rd moult were subjected to different thermal and humidity stress till the assessment of cocoon traits. The comparative rearing and reeling performance clearly indicated that the deleterious effect of high temperature and high RH was more pronounced for the majority of traits such as cocoon uniformity, cocoon weight, shell weight, shell percentage, reelability, filament length, raw silk percentage raw silk recovery denier and waste percentage on silk weight than other temperature and RH treatments and this effect was almost similar for all three silkworm hybrids studied. The present investigation clearly indicate that the deleterious effect of high temperature and high RH was more pronounced on rearing and spinning of silkworm larvae than other temperature and RH treatments and similar effect was noticed for all the three silkworm hybrids studied. The cocoon characters can be improved by providing ideal environmental conditions even during spinning stage of larvae affected with high temperature and RH. The study also suggest that high temperature and low humidity has greater effect during rearing stage than spinning stage.
An Analysis of Genetic Variation and Divergence on Silk Fibre Characteristics of Multivoltine Silkworm (Bombyx mori L.) Genotypes
Kumaresan P. ; Koundinya P. R. ; Hiremath S. A. ; Sinha R. K. ;
International Journal of Industrial Entomology, volume 14, issue 1, 2007, Pages 23~32
The nature of genetic variation and diversity among the 65 multivoltine silkworm genotypes was evaluated for 16 post cocoon characters. The components of genetic variation revealed higher PCV (60.487%) and GCV (44.56%) for evenness (variation 1) followed by cohesion (PCV=55.38%, GCV=40.36%) and non-broken filament length (PCV=32.05%, GCV=31.28%). The higher heritability (
in broad sense) was observed for boil-off loss (95.6%) followed by non-broken filament length (95.22%). The both genotypic and phenotypic correlation indicated significant positive correlation of filament length with non-broken filament length, silk recovery, raw silk, neatness, and low neatness; and negative correlation with denier, renditta and silk waste. The principal component analysis (PCA) revealed 75.381 % of total variance from the five principal components extracted. On the basis of Mahalonobis'
values (Ward's minimum variance), the sixty-five multivoltine silkworm genotypes were classified in to 9 clusters with substantial inter and intra cluster distances. Number of genotypes included in different clusters varied from 3 to 17. The results indicated that the optimum distance obtained in cluster VII (15.059) along with higher cluster mean values especially for filament length, non broken filament length, renditta, silk recovery, silk waste, and raw silk emphasized the utilization of these genotypes in the conventional silkworm breeding programme for improvement of multivoltine silk fibre quality. The possibility of exploiting genetic variation in post cocoon traits for efficient breeding programme is discussed.
Expression and Synergistic Effect of Bacillus thuringiensis CrylAc in Lepidopteran Toxic Strain to Plutella xylostella
Kang, Joong-Nam ; Roh, Jong-Yul ; Shin, Sang-Chul ; Ko, Sang-Hyun ; Chung, Yeong-Jin ; Kim, Yang-Su ; Wang, Yong ; Choi, Hee-Kyu ; Li, Ming-Shun ; Choi, Jae-Young ; Je, Yeon-Ho ;
International Journal of Industrial Entomology, volume 14, issue 1, 2007, Pages 33~36
To improve insecticidal activity of B. thuringiensis 2385-1 (Bt 2385-1), a recombinant plasmid, pHT1K-1Ac, was introduced into lepidopteran toxic Bt 2385-1 by electroporation. The presence of the recombinant plasmid in Bt 2385-1 after electroporation was confirmed by PCR. Bt 2385-1 transformant was named as Bt pHT1K-1Ac/2385-1 (1K-1Ac/2385-1). The 1K-1Ac/2385-1 transformant produced bipyramidal-shaped parasporal inclusion as like the wild-type strain, Bt 2385-1, and showed an 130 kDa band of Cry1Ac protein. The insecticidal activity of 1K-lAc/2385-1 against S. exigua was similar to that of Bt 2385-1 but the
value of transformant against P. xylostella was 1.8 times lower. Through these bioassay results, it was confirmed that toxicity of Bt 2385-1 transformant showed synergistic effect by introducing Cry1Ac. These results suggested that the multiple expressions of Cry proteins in a promising Bt strain may interact synergistically in insect midgut, resulting in increase of toxicity and expansion of host spectrum.
Electrophoretic Analysis of Haemolymph Proteins during Silkworm (Bombyx mori L.) Ontogenesis
Staykova, Teodora ;
International Journal of Industrial Entomology, volume 14, issue 1, 2007, Pages 37~44
A study was made of the haemolymph protein spectrum of mulberry silkworm (Bomhyx mori L.) from the first larval instar to imago. Horizontal starch gel electrophoresis was used. Sixteen races and eight F1 interracial hybrids, raised in Bulgaria, were analyzed. During the ontogenesis, a total of 17 protein bands (15 cathodic and 2 anodic) were detected. Distinct dynamics in the haemolymph protein spectrum was observed, in result of different expression during the individual development associated with the processes of growth, histolysis and histogenesis. Based on the ontogenetic dynamics found, a correspondence was assumed between some proteins detected by us using the starch gel electrophoresis and major haemolymph proteins (SP1, SP2, MHPs and Vg) detected by other authors using the polyacrilamide gel electrophoresis. Intraracial and interracial polymorphism was observed in four protein zones. The effect of four polymorphic loci with codominant and null alleles was suggested.
Botanicals Mediated Reproductive Enhancement in Mulberry Silkworm (Bombyx mori L.)
Kumar P. M. Pratheesh ; Bhargava S.K. ; Prabhakar C.J. ; Kamble C.K. ;
International Journal of Industrial Entomology, volume 14, issue 1, 2007, Pages 45~50
Ethanol extract of 20 botanicals in 3 concentrations i.e., 2.5%, 5% and 10% were evaluated for their efficacy to improve reproductive performance of mulberry silkworm (Bombyx mod L.) through feeding botanical enriched mulberry leaves during
age as well as
age larvae. The preliminary screening was done by ranking the botanicals based on influence in most important reproductive contributing parameters such as single cocoon weight, effective rate of rearing, pupation rate, fecundity and recovery of eggs/kg cocoon. Five botanicals viz., Asparagus recemosus, Achyranthes aspera, Tribulus terrestris, Withania somnifera, Parthenium histerophorus ranked first were further evaluated at 8% 5% and 3% concentrations to confirm their effectiveness and standardize effective concentration. Among the rearing parameters, mature larval weight, shell ratio and number of cocoons/kg were not significantly influenced by the treatments. However, reproductive parameters such as effective rate of rearing (wt), pupation rate (%), fecundity (No.) and egg recovery (g)/kg cocoon were significantly (P<0.05) influenced by the botanicals. Higher fecundity and egg recovery were obtained on treatment with Withania somnifera irrespective of concentrations. However at 5% concentration, pupation rate, fecundity as well as egg recovery were found significantly higher than that of other concentrations, confirming its effectiveness for improving reproductive efficiency.
Apriona germari Larval Cuticle Protein Genes: Genomic Structure of Three Cuticle Protein Genes and cDNA Cloning of a Novel Cuticle Protein
Zheng Gui Zhong ; Kim Bo-Yeon ; Yoon Hyung-Joo ; Wei Ya Dong ; Xijie Guo ; Jin Byung-Rae ; Shon Hung-Dae ;
International Journal of Industrial Entomology, volume 14, issue 1, 2007, Pages 51~56
In a previous study, three larval cuticle protein genes were cloned from the mulberry longicorn beetle, Apriona germari (Comp. Biochem. Physiol. B 136, 803-811, 2003). In the present study, the genomic structures of these three larval cuticle protein genes (AgLCP9.2, AgLCP12.6 and AgLCP12.3) were elucidated. All three cuticle protein genes consist of one intron and two exons. Southern blot analysis of genomic DNA suggested that three cuticle protein genes are a single copy gene. In addition, a novel larval cuticle protein gene, AgLCP10.6, was cloned from A. germari in this study. The AgLCP10.6 cDNA contains an ORF of 300 nucleotides that are capable of encoding a 100-amino acid polypeptide with a predicted molecular mass of 10.6 kDa. The amino acid sequence deduced from the AgLCP10.6 cDNA contained a type-specific consensus sequence identifiable in other insect cuticle proteins and is most homologous to Drosophila melanogaster cuticle protein ACP65A (51 % protein sequence identity). Northern blot analysis revealed that AgLCP10.6 showed epidermis-specific expression.
Screening of Botanicals Against Root Knot Disease Complex in Mulberry (Morus indica L.)
Naik V. Nishitha ; Sharma D.D. ; Govindaiah Govindaiah ; Chowdary N.B. ; Mala V.R. ;
International Journal of Industrial Entomology, volume 14, issue 1, 2007, Pages 57~61
For developing an integrated eco-friendly package against root knot disease complex of mulberry caused by the association of Meloidogyne incognita with Fusarium solani and F. oxysporum causing serious loss in terms of leaf yield and quality during cultivation, twenty botanical extracts at 5, 10 & 20% concentrations were screened under in vitro conditions. Among the extracts, Allium sativum followed by Lasownia inermis were found to be effective at 20% concentration against both the virulent fungi and nematode. Both the extracts reduced the mycelial growth of virulent fungi to an extent of 76-100%, inhibited the hatching of nematode eggs by 80-90% and 76-85% larval mortality over the control. The other extracts were found either moderately or poorly effective in reducing the growth of fungi, hatching of nematode eggs and enhancing the mortality of larvae. The two effective botanical extracts, which rated as strong inhibitors against both nematode and virulent fungi, can be utilized in developing an integrated ecofriendly technology for better management of root knot disease complex in mulberry.
Influence of Different Treatment Regimes of Phytoecdysteroid on Silkworm, Bombyx mori L.
Kumar S. Nirmal ; Nair K. Sashindran ; Mondal Sumana ;
International Journal of Industrial Entomology, volume 14, issue 1, 2007, Pages 63~68
The response of silkworm, Bombyx mori L. to phytoecdysteroid (PE) when administered at different ages of
instar was studied in the popular bivoltine (
) and multi
) silkworm hybrids, reared on the Victory-1 variety of mulberry leaves. PE was administered to
instar silkworm per os at a rate of
per 100 larvae to different batches of silkworm at 0, 24, 48, 72, 96, 120, 144 hrs and at the onset of cocoon spinning when a few larvae were ripe. The larval and mounting duration, cocoon yield and cocoon characters were influenced by PE. The intensity of influence was dependent on the time of application. The larvae treated at the beginning of the instar, improved the economic traits significantly with a marginal increase in larval duration. In the larvae treated at the middle of the instar, larval duration was shortened remarkably but the economic traits were adversely affected. This particular treatment can become a good management strategy in the case of mulberry leaf shortage or disease incidence. In the larvae treated at the onset of cocoon spinning, the mounting duration was substantially reduced without much effect on the cocoon traits which would be a big benefit in commercial sericulture. The physiological significance of varied response of silkworm to PE administration is discussed.