Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
International Journal of Industrial Entomology
Journal Basic Information
Journal DOI :
Korean Society of Sericultural Science
Editor in Chief :
Volume & Issues
Volume 15, Issue 2 - Dec 2007
Volume 15, Issue 1 - Sep 2007
Volume 14, Issue 2 - Jun 2007
Volume 14, Issue 1 - Mar 2007
Selecting the target year
Cloning and Characterization of 6-Phosphogluconolactonase Gene in Silkworm Bombyx mori
Yang, HuaJun ; Chen, KePing ; Yao, Qin ; Guo, ZhongJian ;
International Journal of Industrial Entomology, volume 14, issue 2, 2007, Pages 69~74
As the genome of B. mori is available in GenBank and the EST database of B. mori is expanding, identification of novel genes of B. mori was conceivable by datamining techniques and bioinformatics tools. In this study, we used the in silico cloning method to get the 6-Phosphogluconolactonase (6PGL) gene of B. mori and analysed with bioinformatics tools. The result was confirmed by RT-PCR and prokaryotic expression. The 6PGL cDNA comtains a 702 bp ORF. The deduced protein has 233 amino acid residues, with the predicted molecular weight of 25946. 72 Da, isoelectric point of 5.41, and contains conserved NagB domains. This gene has been registered in GenBank under the accession number EF198104.
Evaluation of some Ethanobotanical Plant Extracts for Fungitoxicity against Myrothecium roridum
Maji, Manas Dev ; Chattopadhyay, Souman ; Kumar, P. M. Pratheesh ; Saratchandra, B. ;
International Journal of Industrial Entomology, volume 14, issue 2, 2007, Pages 75~80
Ethanolic extracts of twenty-one plant species were tested in vitro for their fungitoxic properties against leaf spot causing pathogen of mulberry Myrothecium roridum by poisoned food technique. Ethanolic extracts of twenty-plant spp. reduced mycelial growth of M. roridum significantly except E. pulcherrima. Highest inhibition of M. roridum colony growth observed in 10% extracts of E. citriodora (49.45%) followed by D. metel (39.45%), Chromolaena odoratum (25.56%) and A. sativum (25.00%). Among the concentration tested, 10% concentration was found significantly higher effective on reducing colony growth followed by 5 and 2.5%. Aqueous extract fresh leaves/bulb of seven short-listed plant spp. (inhibition>15% in ethanolic extracts) revealed that D. metel inhibited (23.43%) followed by E. citriodora (14.66%), C. odoratum (13.53%). On dry leaf extracts D. metel was found more effective than E. citriodora. The results indicated that D. metel, E. citriodora, C. odoratum and A. sativum having high fungitoxicity against M. roridum and ethanolic extract found more effective than aqueous extract.
Antimycotic Activity of Allium Sativum Against Beauveria Bassiana, Pathogenic Fungus of White Muscardine Disease in Silkworm, Bombyx mori L. (Lepidoptera: Bombycidae)
Mohanan, N. Madana ; Guptal, S.K. ; Mitra, P. ;
International Journal of Industrial Entomology, volume 14, issue 2, 2007, Pages 81~85
White Muscardine is the most common fungal disease of silkworm, Bombyx mori L. caused by the pathogenic fungus, Beauveria bassiana (Balsamo) Vuillemin. In the present investigation, an attempt has been made to screen locally available medicinal/ weed plants against Beauveria bassiana. Among the plant extracts (PE) tested, 5% aqueous crude extract of the bulb of Allium sativum (Garlic) has been found to be most effective against Beauveria bassiana. The radial growth of Beauveria bassiana in vitro was inhibited to the tune of 54.9% in aqueous extract and 54.4% in ethanolic extract of Allium sativum and correspondingly mycelial dry weight gave rise to 110.7 mg and 108.7 mg against 201.7 mg in control 15 days post treatment. Similarly, silkworm larvae topically inoculated with the Beauveria bassiana conidia (
) registered survival up to 53.0% against 0.0% in control after treatment with aqueous extract of Allium sativum. Simultaneously, as a preventive measure, silkworm larvae were put to rear in conidia contaminated seat paper instantly treated with aqueous extract of Allium sativum that also increased survival up to 61.0% against 4.6% in control. It is also observed that the plant extract is absolutely innocuous to silkworm.
Evaluation of Mulberry Germplasm (Morns spp.) for Leaf Yield and Quality through Bioassay
Tikader, A. ; Kamble, C. K. ;
International Journal of Industrial Entomology, volume 14, issue 2, 2007, Pages 87~92
Twenty - four elite mulberry germplasm each of indigenous and exotics were studied for their leaf yield and compared with commercial check (
and Kosen). Accession MI-0416 and ME-0169 out yielded the check accession in leaf yield/plant. The other few mulberry germplasm were also performed at par with the checks. For quality test and bioassay were conducted with the leaves of selected mulberry germplasm. Among the selected twelve mulberry accessions used for bioassay, MI-0376 and ME-033 performed better than check (
, Kosen). Other mulberry accessions i.e., MI-0310 and MI-0437 are on par with the check as far as the bioassay is concerned. MI-0376 and ME-0033 out yielded in rearing parameters and qualified for 11 and 10 rearing and related traits. Other mulberry accessions i.e., MI-0310 and MI-0437 were also qualified for eight rearing traits along with check (
). The mulberry accessions tested after selection from the preliminary characterization seems to be better and equally good in rearing and leaf yield compared to check (
, Kosen), which provides scope for selection and further evaluation. The selected mulberry accession may be included in crop improvement programme.
Development of New Robust Bivoltine Silkworm Hybrid
for Rearing throughout the Year
Rao, P. Sudhakara ; Nayaka, A. R. Narasimha ; Mamatha, M. ; Sowmyashree, T. S. ; Bashir, Ifat ; Ilahi, Irfan ;
International Journal of Industrial Entomology, volume 14, issue 2, 2007, Pages 93~97
A Silkworm breeding programme was designed to develop a robust but productive bivoltine silkworm hybrid of Bombyx mori L. suitable for rearing throughout the year in tropical climate by utilizing indigenous polyvoltine and productive bivoltine breeds. The breeding was carried out under high temperature (
) and low humidity (
) conditions in the environmental chamber. By
, three oval and three dumbbell breeds were isolated with higher survival and productive merits. These breeds were utilized in the hybrid evaluation along with other popular breeds. Based on combining ability test results, the hybrid
was selected for large scale testing and evaluated in different seasons. The evaluation studies indicated that the hybrid has higher viability and productive merits and it is suitable to rear throughout the year. The hybrid
recorded a survival of 92.0%, cocoon shell weight of 0.417 g, cocoon shell percentage of 23.0 and a filament length of 1042 meters under hot and dry conditions of environmental chamber compared to the control thermo-tolerant hybrid
Breeding of Bivoltine Breeds of Bombyx mori L Suitable for Variable Climatic Conditions of the Tropics
Moorthy, S. M. ; Das, S. K. ; Kar, N. B. ; Urs, S. Raje ;
International Journal of Industrial Entomology, volume 14, issue 2, 2007, Pages 99~105
The success of rearing with presently available conventional bivoltine is unpredictable in some seasons of the tropical regions due to highly fluctuating adverse climatic conditions. Thus, in order to popularize bivoltine breeds in tropical parts of India, it is very much essential to have a bivoltine breed(s), which can give stable cocoon crop under variable environments. With this objective a breeding programme was undertaken to improve the survival trait in bivoltine silkworm by introducing multivoltine genes into bivoltine through back crossing. Resultant bivoltine lines showed significantly higher survival in compared to the receptor (Bivoltine) parent and control bivoltine breed. Esterase isozyme analysis revealed similar banding pattern in the developed bivoltine and in the donor multivoltine, which predicts the introgression of multivoltine character into evolved bivoltine.
Mitochondrial DNA Sequence Variation of the Oriental Mole Cricket, Gryllotalpa orientalis (Orthoptera: Gryllotalpidae) in Korea
Kim, Ik-Soo ; Cha, So-Young ; Lee, Sun-Young ; Kim, Seong-Ryul ; Hwang, Jae-Sam ; Li, Jianhong ; Han, Yeon-Soo ; Jin, Byung-Rae ;
International Journal of Industrial Entomology, volume 14, issue 2, 2007, Pages 107~112
The mole crickets, Gryllotalpa, are insect pest distributed in the world. In Korea, G. orientalis was reported to occur, but previous ecological studies suggested the presence of two ecological types. To test this hypothesis, we sequenced a portion of mitochondrial (mt) genome from 48 G. orientali individuals collected over five Korean localities: Busan, Suwon, Okchon, Wonju, and Gangneung. From the sequence analysis, only two haplotypes were obtained, but the sequence divergence between the two haplotypes was 11 %, suggesting the presence of two distinct genetic groups in Korea. Although the population of Busan, Okchon, Wonju, and Gangneung was identified as a single haplotype, but that of Suwon was occupied by both hapotypes. Considering sequence divergence of other insect species occurring in Korea, the divergence estimate found between the two haplotypes seems to be too large to be considered as identical species. This result may suggest that the two differentiated haplotypes found in this study may reflect the previously reported two ecological types found in Suwon, Korea. To further understand the genetic divergence of the two phylogenetic groups, analysis of more variable regions of G. orientalis genome is required.
Effect of Mating Delay and Mating Duration on Reproductive Performance of Antheraea mylitta
Rath, S. S. ; Narain, Raj ; Singh, M. K. ; Suryanarayana, N. ;
International Journal of Industrial Entomology, volume 14, issue 2, 2007, Pages 113~119
Tropical tasar silkmoth Antheraea mylilta D. is an economically important insect. The wild nature and above all unsynchronized emergence of moths lead to impairment in reproductive activity. The mating in moths is low (64%-70%), leaving about 30-36% of potential females unused for seed (= egg) production. Delay in mating adversely affects the reproductive performance such as mating behaviour, fecundity and fertility. Females are more severely affected by mating delay than males. Therefore, a study was under taken to develop a method to overcome the problem so that the production of layings can be increased to meet the ever increasing demand. It was revealed from the study that out of 16 possible age combinations (from the age groups of day 0 to day 3), female and male of day 0 (4-8 hours old) excel in their reproductive performance than others (with 94.4% mating success, 284 fecundity and 91.5% hatching). Reproductive performance declined significantly even if one of the parents was older. When either of the mating pair was 1 day old, the mating declined by 28%; fecundity by 32.8%; and hatching by 40.8%. The insemination of eupyrene sperm was declined by 35.2% when both the parents are 1 day old. Mating duration of 8 hours resulted in insemination of
eupyrene sperms, which was declined by 31.6%, 48% and 55% upon lowering of mating duration to 6 hr, 4 hr and 2 hr were maintained respectively.
Isaria sinclairii Extract Reduces Body Weight and Ameliorates Metabolic Abnormalities
Ahn, Mi-Young ; Kim, Ji-Young ; Han, Jae-Woong ; Jee, Sang-Duck ; Hwang, Jae-Sam ; Cho, Sung-Ig ; Yun, Eun-Young ;
International Journal of Industrial Entomology, volume 14, issue 2, 2007, Pages 121~126
Obesity is a major risk factor for cardiovascular disease. In our case study using animal models for disease states such as obesity or hypertension, we found that, Isaria sinclairii remarkably reduced body weight and ameliorated metabolic abnormalities in Zucker and SHR rats. Genetically obese (fa/fa) Zucker rats were one animal model chosen for this study. Four groups of rats received a standard diet and were treated orally with the following test samples daily for 8 weeks: saline (negative control), ethanol extracts of I. sinclairii, hot water extract of I. sinclairii, or
(30 mg/kg, positive control). Mild reductions (6.3%) in body weight gain were observed in the groups treated with the hot water extract of I. sinclairii compared to the control after 8 weeks. Interestingly, organ weight was greatly reduced by this Dongchunghacho (I. sinclairii), in parallel with the mild reductions in body weight gain and reductions in abdominal fat (adipose tissue). Also observed was a 4.1 % decrease in the ratio of heart weight/body weight compared to the control group. As a hypertensive animal model, SHR (spontaneously hypertensive rat) and WKY (Wistar Kyoto) rats were also administered these extracts for one month. Treatment with the hot water extract of I. sinclairii caused greater reductions in body weight gain for the SHR group (10.9%) compared to the WKY group's (5.2%). Based on these results, I. sinclairii extracts contain selective action for anti-obesity activity, naturally occurring candidate for regulation of body weight increase, as demonstrated in the present study.
Antijuvenoid Action of Terpenoid Imidazole Compound on Larval - Pupal - Adult Development of Silkworm, Bombyx mori L.
Saha, Atul Kumar ; Datta (Biswas), Tapati ; Das, Salil Kumar ; Kar, Niharendu Bikash ;
International Journal of Industrial Entomology, volume 14, issue 2, 2007, Pages 127~135
Precocious metamorphosis was induced in two popular bivoltine breeds i.e. YB and
by an imidazole compound having anti-juvenile hormone activity. The chemical was administered by feeding treatment with mulberry leaf to freshly moulted (0-6 hrs) IV instar larvae. The dose of the chemical was found to be breed specific being 650 ppm for YB and 500 ppm for
. The chemical caused complete skipping up of the fifth instar larvae which is most susceptible to diseases. But IV instar was somewhat prolonged. As a result the total larval period was reduced by 4-5 days. However, some of the rearing and reeling parameters like cocoon yield, cocoon weight, shell weight, shell %, filament length and fecundity were reduced for that particular generation only. The effective rate of rearing (ERR %) was significantly increased in trimoulters during the most unfavourable August - September seed crop season. Number of cocoons / kg and number of male moth were significantly higher in trimoulters. These may be useful for preparation of
hybrid seed. Fine denier was also found in trimoulter cocoon which is the cause of getting fine silk filament from trimoulter cocoons. Normal mating behaviour and emergence pattern was recorded in trimoulters. Bivoltine trimoulter males also showed competence for mating with multivoltine females. These results suggest the possibility of getting trimoulter males during hot and humid seasons when rearing of bivoltine is almost impossible particularly in Eastern and North Eastern India.
Antibacterial Activity of the Honey Bee Venom against Bacterial Mastitis Pathogens Infecting Dairy Cows
Han, Sang-Mi ; Lee, Kwang-Gill ; Yeo, Joo-Hong ; Kweon, Hae-Yong ; Kim, Bong-Soon ; Kim, Jae-Myung ; Baek, Ha-Ju ; Kim, Soon-Tae ;
International Journal of Industrial Entomology, volume 14, issue 2, 2007, Pages 137~142
The antibacterial activity of Korean honey bee venom (KBV) was examined against seven major bacterial mastitis pathogens, Enterococcus faecium, Escherichia coli, Methicillin resistant Staphylococcus aureus (MRSA), Staphylococcus aureus, Streptococcus intermedius, Streptococcus oralis and Streptococcus uberis isolated from infected mammary quarters of cows. Seven bacterial mastitis pathogens were studied for antibacterial activity of the KBV by disc diffusion assay, minimal inhibitory concentrations (MIC) and bacterial count in milk samples. The KBV showed activity against Ent. faecium, E. coli, MRSA, Staph. aureus, Strep. intermedius. The order of susceptibility of the bacteria against the KBV was Staph. aureus>MRSA>E. coli>Strep. intermedius>Ent. faecium>Strep. oralis>Strep. uberis. The MIC against Staph. aureus, MRSA and E. coli were stronger effect as compared with standard drug. The effect of the KBV (
) on the viability of Ent. faecium, E. coli, MRSA, Staph. aureus, Strep. intermedius, Strep. oralis and Strep. uberis in milk differed significantly with each other within 12 h incubation period. The results indicate that KBV has significant antibacterial effects against major bacterial mastitis bacteria, Ent. faecium, E.coli, MRSA, Staph. aureus, Strep. intermedius. Results of the study indicate the potential use of KBV as alternative to antibiotic therapy. Further investigations are needed though to confirm its efficacy and its effects on the animals.
Compatibility of Entomopathogenic fungus Lecanicillium attenuatum and Pesticides to control Cotton Aphid, Aphis gossypii
Kim, Jeong-Jun ; Kim, Kyu-Chin ;
International Journal of Industrial Entomology, volume 14, issue 2, 2007, Pages 143~146
Concerns of entomopathogenic fungi as alternative pest control agents are increasing even though chemical pesticides have been used as the main control agents for pests and diseases in crop production. This study was conducted to test the influence of fungicides and insecticides on an isolate of Lecanicillium attenuatum that was reported to have the pathogenicity against cotton aphid, because fungicides and/or insecticides can apply with mycopesticides simultaneous, before and/or after. Fungicides fenbuconazole+thiram and propineb inhibited the spore germination and mycelial growth of L. attenuatum CS625; dimethomorph and procymidone did not affect spore germination or mycelial growth. The insecticide abamectin, deltamethrin, imidachropride, and spinosad had no detrimental effects on spore germination or mycelial growth. Therefore, these results demonstrated that careful selection of pesticides and fungicides can be applied to the integrated pest and disease control with microbial pesticide.
Antibacterial Activity of Peptides Synthesized Based on the Bombus ignitus abaecin, A Novel Proline-Rich Antimicrobial Peptide
Kim, Seong-Ryul ; Lee, Eun-Mi ; Yoon, Hyung-Joo ; Choi, Yong-Soo ; Yun, Eun-Young ; Hwang, Jae-Sam ; Jin, Byung-Rae ; Lee, In-Hee ; Kim, Ik-Soo ;
International Journal of Industrial Entomology, volume 14, issue 2, 2007, Pages 147~150
Abaecin is a largest member of the proline-rich anti-microbial peptide family found only in the hymenopterans. A cDNA of abaecin was previously isolated and cloned from Bombus ignitus: the mature peptide of Bombus ignitus abaecin was composed of 39 amino acid residues. In the present study, we determined the antibacterial effect of B. ignitus abaecin synthesized at several lengths against several bacteria by radial diffusion assay. The 37-mer peptide (Ab37) inhibited the growth of Gram-negative bacteria Escherichia coli ML-35, Pseudomonas aeruginosa and Salmonela typhimurium, but showed limited inhibitory activity toward Gram-positive bacteria, except for Micrococcus luteus. The truncated 26-mer peptide (Ab26), which was synthesized after truncating some amino acid residues at both N-terminus and C-terminus from the Ab37 peptide, still showed equivalent antibacterial activity to the Ab37. On the other hand, several further truncated peptides exhibited lower activity then did Ab37 peptide.