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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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International Journal of Industrial Entomology
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Journal DOI :
Korean Society of Sericultural Science
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Volume & Issues
Volume 15, Issue 2 - Dec 2007
Volume 15, Issue 1 - Sep 2007
Volume 14, Issue 2 - Jun 2007
Volume 14, Issue 1 - Mar 2007
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Egg Diapause Induction in Multivoltine Silkworm Bombyx mori for Long-term Germplasm Preservation
Kumar, R. Saravana ; Ponnuvel, K.M. ;
International Journal of Industrial Entomology, volume 15, issue 1, 2007, Pages 1~7
At present, multivoltine silkworm races reared five times per year involving huge manpower and rearing expenditure. Egg diapausing behavior is facultative in multivoltine and egg diapause was induced in selected multivoltine races by regulating temperature at
, relative humidity 80% and photoperiod (6L:18D) in the late stage silkworm rearing. The maximum percentage of egg diapause induction was recorded in Rong Diazo, Diazo and MW13 showed 94%, 93% and 92% respectively, whereas the races A14DY and OS-616 showed minimum diapause induction 15% and 18% respectively. The diapause induced multivoltine eggs were preserved up to six months by cold preservation schedule normally adopted for bivoltine. After three and six months egg preservation, the diapause induced layings were released and observed for hatching percentage, all races showed above 82 % of hatching except the race AP12, which showed only 78 % of hatching. This methods reduce the crop cycle, gives strong safety backup and preventing the genetic erosion. This study helps formulating a new conservation method for multivoltine silkworm germplasm.
In Vivo Effects of Antibiotics on Silkworm Bombyx Mori L Infected with Bacillus Coagulans
Savithri, G. ;
International Journal of Industrial Entomology, volume 15, issue 1, 2007, Pages 9~16
The present study was aimed to screen different antibiotics in vivo for their effects against the bacterium Bacillus coagulans in silkworm Bombyx mori in three experimental conditions viz., a) healthy larvae treated with the antibiotics b) larvae which were first inoculated with Bacillus coagulans immediately after the
moult and treated with the antibiotics from 24 hour after inoculation up to the end of the
instar(pre-inoculated larvae) and c) larvae which were treated with the antibiotics right from hatching up to the end of the
instar and inoculated with Bacillus coagulans immediately after the
moult(post-inoculated larvae). All the antibiotics used in this study stimulated better performance in improving the rearing performance and economic characters of the cocoons in healthy, pre-inoculated and post-inoculated larvae, besides reducing the mortality of infected larvae. Cephelexin and Tetracycline were more effective than the others in their overall performance under all the three experimental conditions. Chloramphenical caused greater increase in the length of the reelable silk filament, but was not as good in elevating the other parameters compared to the remaining antibiotics. Of the four concentrations of antibiotics tested, 2.0% performed better under all the three experimental conditions.
Evaluation of Thermo Tolerance of 'Nistari' an Indigenous Strain of Multivoltine Silkworm, Bombyx mori L.
Moorthy, S.M. ; Das, S.K. ; Mukhopadhyay, S.K. ; Mandal, K. ; Urs, S. Raje ;
International Journal of Industrial Entomology, volume 15, issue 1, 2007, Pages 17~21
An indigenous multivoltine silkworm, Nistari was evaluated for their thermo tolerance by exposing the larvae to various temperature regimes for eight hours. Among different temperature exposed, this strain has significant tolerance at
. Analysis of heat shock protein revealed the expression of 70 kDa and 64 kDa polypeptides in fat body and midgut tissues. Interestingly esterase isozyme pattern in midgut showed characteristic expression of Est-1 and Est-3 at different temperatures signifying role in heat and cold shock.
Cryopreservation of Mulberry Germplasm Core Collection and Assessment of Genetic Stability through ISSR Markers
Rao, A. Ananda ; Chaudhury, Rekha ; Kumar, Suseel ; Velu, D. ; Saraswat, R.P. ; Kamble, C.K. ;
International Journal of Industrial Entomology, volume 15, issue 1, 2007, Pages 23~33
A simple and reliable cryo technique using desiccation and slow freezing of winter dormant buds was employed for 238 core collection of mulberry germplasm collected from diverse geographical regions and maintained under tropical conditions in the ex situ field gene bank to develop long-term biodiversity conservation for ensuring sustainable utilization of these valuable resources. Desiccation and freezing tolerance of bud grafts and excised shoot apices in the axillary buds of different Morus species under in vivo and in vitro condition indicated species-specific variation and most of the wild Morus species were found sensitive. In vitro regeneration and cryopreservation(
) protocols using differentiated bud meristem like axillary winter dormant buds were worked out for a wide range of Morus species, land races, wild and cultivated varieties. Successful cryopreservation of mulberry winter dormant buds of different accessions belonging to M. indica, M. alba, M. latifolia, M. cathayana, M. laevigata, M. nigra, M. australis, M. bombycis, M. sinensis, M multicaulis and M. rotundiloba was achieved. Among wild species Morus tiliaefolia, and M. serrata showed moderate recovery after cryopreservation. Survival rates did not alter after three years of cryopreservation of different Morus species. ISSR markers were used to ascertain the genetic stability of cryopreserved mulberry, which showed no difference detected among the plantlets regenerated from frozen apices in comparison to the non-frozen material.
Rapid Expression of Bm46 in Bombyx mori Cell Lines, Larvae and Pupae
Wang, Haiyan ; Chen, Keping ; Guo, Zhongjian ; Yao, Qin ; Wang, Qiang ; Mu, Runhong ;
International Journal of Industrial Entomology, volume 15, issue 1, 2007, Pages 35~38
In this study, ORF 46 of Bombyx mod nucleopolyhedrovirus(Bm46) fused with EGFP was expressed in Bombyx mod cell lines, larvae and pupae by BmNPV Bacmid system. Bm46 and EGFP were cloned into donor plasmid pFastBacHTb, which was transformed to competent DH10B cells containing helper and BmNPV bacmid by site-specific transposition. Recombinant bacmid was used to transfected BmN-4 cells to produce the recombinant baculovirus vBm-Bm46-EGFP. Recombination virus was injected into silkworm larvae and pupae. The expression of the fusion protein was monitored by examining green fluorescence using a fluorescent microscope. Intense fluorescence in cells and silkworm was observed at 4 days post-infection, indicating the Bm46-EGFP fusion gene was expressed successfully.
The Effect of Vespa simillima Extracts on Long-Term Memory and MK-801-Induced Learning Disability in Mice
Fujiwara, Yumiko ; Kobayashi, Haruo ; Kawai, Shigenao ; Suzuki, Koichi ;
International Journal of Industrial Entomology, volume 15, issue 1, 2007, Pages 39~45
Extracts of adult worker bodies of Vespa simillima in 2 % NaCl or acidified methanol were administered orally to mice for 70 days. Following this period, memory at one-day and one-month periods, and the effects on scopolamine-induced amnesia were examined using a step-through passive avoidance task. Changes in MK-801-induced disability after 8 days of training, and in memory one month after the trial were also assessed. Mice treated with the 2% NaCl extract showed significant improvement in memory in the behavioral tests one month after the trial, whereas mice receiving the extract in acidified methanol, did not differ from the controls in any trial. The results inidicate that Vespa simillima contains substances acting favorably on the cerebral functions of mammals.
Mitochondrial DNA Sequence Variation of the Tiny Dragonfly, Nannophya pygmaea(Odonata: Libellulidae)
Kim, Ki-Gyoung ; Jang, Sang-Kyun ; Park, Dong-Woo ; Hong, Mee-Yeon ; Oh, Kyoung-Hee ; Kim, Kee-Young ; Hwang, Jae-Sam ; Han, Yeon-Soo ; Kim, Ik-Soo ;
International Journal of Industrial Entomology, volume 15, issue 1, 2007, Pages 47~58
The tiny dragonfly, Nannophya pygmaea(Odonata: Libellulidae) is one the smallest dragonflies in the world and listed as a second-degree endangered wild animal and plant in Korea. For the long-term conservation of such endangered species, an investigation on nation-wide genetic magnitude and nature of genetic diversity is required as a part of conservation strategy. We, thus, sequenced a portion of mitochondrial COI gene, corresponding to "DNA Barcode" region(658 bp) from 68 N. pygmaea individuals collected over six habitats in Korea. The sequence data were used to investigate genetic diversity within populations and species, geographic variation within species, phylogeographic relationship among populations, and phylogenetic relationship among haplotypes. Phylogenetic analysis and uncorrected pairwise distance estimate showed overall low genetic diversity within species. Regionally, populations in southern localities such as Gangjin and Gokseong in Jeollanamdo Province showed somewhat higher genetic diversity estimates than those of remaining regions in Korean peninsula. Although geographic populations of N. pygmaea were subdivided into two groups, distance- or region-based geographic partition was not observed.
Shifts in Protein Metabolism in Hemolymph and Fat Body of the Silkworm, Bombyx mori L. in Response to Fluoride Toxicity
Ramakrishna, S. ; Jayaprakash, Jayaprakash ;
International Journal of Industrial Entomology, volume 15, issue 1, 2007, Pages 59~68
Changes in protein metabolism were studied in hemolymph and fat body on days 1, 3, 5 and 7 of the fifth-instar silkworm, Bombyx mori, exposed to lethal, sublethal doses and prevailing levels of fluoride in groundwater in Karnataka and Andhra Pradesh States of India. The total protein content indicated a depletion followed by a concomitant increase in accumulation of free amino acids. Concurrently, the activity of protease in both of the tissues was also increased. A steady enhancement in the activities of alanine aminotransferase and aspartate aminotransferase paralleled the elevation of glutamate dehydrogenase activity in the tissues studied. It is presumed, on the basis of these results, that the fluoride toxicity causes major changes in protein metabolism of the silkworms.
Effect of Temperature on Development and Reproduction of the Emma Field Cricket, Teleogryllus emma(Orthoptera: Gryllidae)
Kim, Nam-Jung ; Hong, Seong-Jin ; Seol, Kwang-Youl ; Kim, Seong-Hyun ; Ahn, Nan-Hee ; Kim, Mi-Ae ;
International Journal of Industrial Entomology, volume 15, issue 1, 2007, Pages 69~73
To establish the indoor-rearing system of the emma field cricket, Teleogryllus emma, the effects of temperature on development and reproduction of the emma cricket have been studied. The influence of temperature on developmental periods of emma field cricket was investigated under the eight temperatures of 15, 18, 21, 25, 27, 29, 31 and
R.H. and 16L:8D photoperiod. The developmental periods of T. emma nymphs had a range of 124.8 days to 44.4 days at the temperature of
, respectively. At
, however, all tested individuals died before emergence. The highest survival rate was 90% at
, but there were no statistically significant differences among the temperatures. The adult weight increased with increasing temperatures although the weight at
was decreased. In addition, the influence of temperature on reproduction of emma field cricket was investigated under three temperatures
R.H. and 16L:8D photoperiod. The longevity of female/male adults were 65.8/79.2 days, 68.5/67.8 days, 46.8/57.4 days at the temperature
, respectively. The preoviposition periods were 32.5 days at
and 22.1 days at
. The highest average fecundity per female was 737.3 at
Molecular Characterization of Small Heat Shock Protein(hsp20.8A) from the Silkworm, Bombyx mori
Hwang, Jae-Sam ; Go, Hyun-Jeong ; Goo, Tae-Won ; Seong, Su-Il ; Yun, Eun-Young ; Ahn, Mi-Young ; Kim, Seong-Ryul ; Park, Kwan-Ho ; Kim, Ik-Soo ; Jeon, Jae-Pil ; Kang, Seok-Woo ;
International Journal of Industrial Entomology, volume 15, issue 1, 2007, Pages 75~78
To define the molecular mechanism of initiation and termination of diapause during the embryogenesis of silkworm, Bombyx mori, mRNA transcripts from diapausing eggs and diapause activated eggs were compared with differential expression using cDNA array. Among those clones, mRNA transcript from hsp20.8A, which was expressed at a high level in diapausing eggs that had been incubated at
for 30 days after oviposition, whereas, in the eggs exposed to
for 30 days,
for 60 days, the expression of mRNA decreased. On the other hand, the expression of mRNA during embryogenesis observed abundantly at 4 to 6 days after heat-HCl treatment and later at 9 to 10 days after just before hatching. This result was suggested for us that hsp20.8A was expressed in response to embryogenesis as well as physical stress.
Evaluation of Lac Cultivation in Two South-Western Districts of Bangladesh
Bahar, Habibullah ; Islam, Tarikul ; Islam, Monirul ; Mannan, Abdul ;
International Journal of Industrial Entomology, volume 15, issue 1, 2007, Pages 79~82
Lac is the dermal secretion of lac insect, Kerria lacca Kerr., which is used to make expensive natural dye, burnish, coating materials, in cosmetics and jewelry industry. Though it is very perspective industry and available of host plants over the country especially in southern districts of Bangladesh, lac cultivation is confined within some northern districts of Bangladesh. Experiment was conducted to find out the possibility of lac cultivation in two southwestern districts of Bangladesh: Khulna and Satkhira compared to Chapainowabgonj, the key lac producing district in Bangladesh, during February-October, 2006. The bark thickness, bark weight of ber plant, ratio of harvested and inoculated lac sticks, harvested raw lac, and harvested processed lac were measured and compared. Statistically, similar performance of lac cultivation was found regarding all parameters in three districts. These results recommended that lac cultivation is possible at the south-western coastal part of Bangladesh.
Molecular Characterization of A Glycine and Proline-rich Antibacterial Protein from Larvae of A Beetle, Protaetia brevitarsis
Hwang, Jae-Sam ; Kim, Seong-Ryul ; Kang, Heui-Yun ; Yun, Eun-Young ; Ahn, Mi-Young ; Park, Kwan-Ho ; Jeon, Jae-Pil ; Kim, Mi-Ae ; Kim, Nam-Jung ; Hwang, Seok-Jo ; Kim, Ik-Soo ;
International Journal of Industrial Entomology, volume 15, issue 1, 2007, Pages 83~85
A glycine and proline-rich antibacterial protein was cloned from larvae of a beetle, Protaetia brevitarsis. The DNAs encoded a deduced propeptide of 127 amino acid residues with predicted molecular weight of 14.0 kDa and PI of 7.89. Structural analysis of this protein indicated the presence of a recognition sequence for the cleavage site within the constitutive secretory pathway(Arg-Xaa-Lys/Arg-Arg), suggesting that mature portion(72 amino acid residues) is produced by cleavage of signal peptide and propeptide from 127 amino-acid-long precursor protein. Mature portion sequence of this protein showed 72% similarity to that of Oryctes rhinoceros Rhinocerosin and 91% to that of Holotrichia diomphalia holotricin 2. The mRNA expression was reached the highest level at 4 hrs after E. coli injection and then declined gradually.
Test of Larvicidal Effect of Some Commercial Natural Products on Lepidoptran Plutella xylostella and Spodoptera litura Larvae
Jeong, Hyung-Uk ; Im, Hyun-Hwak ; Chang, Sung-Kwon ; Paik, Chae-Hoon ; Han, Tae-Ho ; Kim, In-Seon ; Kim, Ik-Soo ;
International Journal of Industrial Entomology, volume 15, issue 1, 2007, Pages 87~91
A diverse kind of environment-friendly agricultural materials(EFAM) for the control of insect pests is on the market. These EFAMs are a part of essential sources for the accomplishment of successful, sustainable, and environment-friendly agriculture. Thus, accurate information of these EFAMs is one that required for the success of environment-friendly agriculture, but, in reality, still appropriate information is absolutely in shortage. In this study, we, therefore, tested the efficacy of commercial EFAMs against two lepidopteran insect larvae, the diamondback moth Plutella xylostella(Lepidoptera: plutellidae) and the tobacco cutworm Spodoptera litura(Lepidoptera: Noctuidae). After the two insect pests were successfully stabilized in indoor environment the larvicidal activity was tested at
, relative humidity(RH) of
, and a photoperiod of 16L:8D, and mortality was determined 48 hrs after EFAMs are treated. The EFAMs that showed more than 90% of larvicidal activity were each six among 16 against both P. xylostella and S. litura and only three of them showed consistent larvicidal activity against both species, signifying species specificity of EFAMs and importance of selection of proper EFAMs depending on target insect pest.