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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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International Journal of Industrial Entomology
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Korean Society of Sericultural Science
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Volume & Issues
Volume 15, Issue 2 - Dec 2007
Volume 15, Issue 1 - Sep 2007
Volume 14, Issue 2 - Jun 2007
Volume 14, Issue 1 - Mar 2007
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Intronic Sequences for the Discrimination of Silkworm Strains in Silkworm Powder
Kim, Kee-Young ; Lee, Eun-Mee ; Hong, Mee-Yeon ; Kang, Pil-Don ; Ryu, Kang-Sun ; Kim, Ik-Soo ;
International Journal of Industrial Entomology, volume 15, issue 2, 2007, Pages 93~100
After a serial study on the therapeutic efficacy of the powdery silkworm for diabetics was positively resulted in, many powdery silkworm products were on the market in Korea. Up to now, however, no causal method is available to discriminate the strain of silkworms that is a major ingredient for manufacturing powdery silkworm, even though the quality of the powdery silkworm differs greatly by source and origin of strains. We previously were successful in identifying 25 silkworm strains kept in Korea using nine intronic sequences. In this study, we tested the utility of the nine intronic sequences to distinguish the most widely reared silkworm strains originated from Korea and China. Two intron regions, PTTH Intron3 and PTTH Intron3, showed a substantial sequence divergence (mean sequence divergence of 3.13% in PTTH Intron3 and 4.99% in PTTH Intron3). These two intronic sequences provided no identical sequences among the seven strains tested. Thus, these sequences each along can be used to discriminate the seven strains tested in this study. Furthermore, other intron regions, except for VDP Intron4 allowed us to discriminate
strains by strain-specific unique insertion/deletion or substitution.
Efficacy of Pesticides and Growth Hormones against Root Disease Complex of Mulberry (Morus alba L.)
Naik, Vorkady Nishitha ; Sharma, Dinesh Dutta ;
International Journal of Industrial Entomology, volume 15, issue 2, 2007, Pages 101~106
During mulberry cultivation, root disease complex caused by the association of root knot nematode (Meloidogyne incognita) with root rot pathogens like Fusarium solani and Botryodiplodia theobromae poses serious loss in leaf production. Therefore, an attempt was made to assess the efficacy of eight pesticides (Metayalaxyl+Mancozeb, Thiophanate methyl, Mancozeb, Bitertanol, Phenomiphos, Phorate, Thionazin & Carbofuran) and two growth hormones (Salicylic acid and Indole 3 acetic acid) at 0.1 and 0.2% concentrations under in vitro conditions against nematode (hatching of eggs and mortality of larvae) and root rot pathogens (poisoned food technique) for short listing the treatments to develop an IDM strategy. Results revealed that among the pesticides and growth hormones, Carbofuran followed by Salicylic acid were found to be effective at 0.2% concentration against both nematode and pathogenic fungi. Both the chemicals inhibited the hatching of nematode eggs by 83.5-78.9% and 80-76% larval mortality over the control and reduced the mycelial growth of both the pathogenic fungi to an extent of 75.5-77.8%. Though Mancozeb inhibited both the pathogenic fungi strongly (77-80%), it did not show any effectiveness against nematode. The rest of the chemicals were found either moderately or poorly effective in reducing the growth of pathogenic fungi, hatching of nematode eggs and enhancing the mortality of larvae. The two effective chemicals viz., Carbofuran and Salicylic acid, which rated as strong inhibitors against both nematode and pathogenic fungi, can be exploited in developing an IDM package as one of the component for better management of root disease complex in mulberry.
Mating Conditions Favorable for Improving Mating Rate of the Bumblebee, Bombus ignitus
Yoon, Hyung-Joo ; Kim, Sam-Eun ; Lee, Kyeong-Yong ; Lee, Sang-Beom ; Park, In-Gyun ;
International Journal of Industrial Entomology, volume 15, issue 2, 2007, Pages 107~114
We investigated mating conditions of photoperiod, illumination and temperature during mating periods, care temperature of queen before mating, mating period and number of queen per mating cage to improve mating rate of Bombus ignitus. Among photoperiodic regimes of 12L, 14L and 16L during mating periods, queen mated at 14L showed better results than at 12L and 16L in egg-laying characteristics and colony development. In case of illumination during mating periods, intensity of 1000 lux was more effective than at intensity of 100 lux and 2000 lux in mating B. ignitus queen. Mating temperature and care temperature of queen before mating favorable for B. ignitus queen were
, respectively. The period need to mating B. ignitus queen was 3 days, and the number of queen suitable per mating cage of
Molecular Cloning, Bioinformatics Analysis and Expression Profiling of a Gene Encoding Vacuolar-type
Synthetase (V-ATPase) c Subunit from Bombyx mori
Lu, Peng ; Chen, Keping ; Yao, Qin ; Yang, Hua-Jun ;
International Journal of Industrial Entomology, volume 15, issue 2, 2007, Pages 115~122
As the genome of B.mori is available in GenBank and the EST database of B.mori is expanding, identification of novel genes of B.mori is conceivable by data-mining techniques. We used the in silico cloning method to get the vacuolar-type
synthetase (V-ATPase) c subunit (16 kDa proteolipid subunit) gene of B.mori and analysed with bioinformatics tools. The result was confirmed by RT-PCR and sequencing. The V-ATPase c subunit cDNA contains a 468 bp ORF. The ORF encoded a 155-residue protein that showed extensive homology with V-ATPase c subunits from other 15 species and contained four membrane-spanning helices. Tissue expression pattern analysis revealed that V-ATPase c expressed strongly in Malpighian tubules, not in fat body. This gene has been registered in GenBank under the accession number EU082222.
Genomic Organization of Heat Shock Protein Genes of Silkworm Bombyx mori
Velu, Dhanikachalam ; Ponnuvel, Kangayam M. ; Qadri, Sayed M. Hussaini ;
International Journal of Industrial Entomology, volume 15, issue 2, 2007, Pages 123~130
The Hsp 20.8 and Hsp 90 cDNA sequence retrieved from NCBI database and consists of 764 bp and 2582 bp lengths respectively. The corresponding cDNA homologus sequences were BLAST searched in Bombyx mori genomic DNA database and two genomic contigs viz., BAAB01120347 and AADK01011786 showed maximum homology. In B. mori Hsp 20.8 and Hsp 90 is encoded by single gene without intron. Specific primers were used to amplify the Hsp 20.8 gene and Hsp 90 variable region from genomic DNA by using the PCR. Obtained products were 216 bp in Hsp 20.8 and 437 bp in Hsp 90. There was no variation found in the six silkworm races PCR products size of contrasting response to thermal tolerance. The comparison of the sequenced nucleotide variations through multiple sequence alignment analysis of Hsp 90 variable region products of three races not showed any differences respect to their thermotolerance and formed the clusters among the voltinism. The comparison of aminoacid sequences of B. mori Hsps with dipteran and other insect taxa revealed high percentage of identity growing with phylogenetic relatedness of species. The conserved domains of B. mori Hsps predicted, in which the Hsp 20.8 possesses
domain and Hsp 90 holds HATPase and Hsp 90 domains.
Expression of Bombyx mori Nucleopolyhedrovirus ORF4 under the Control of BaculoviruS Ie1 Promoter by a Novel Bac-to-Bac/BmNPV Baculovirus Expression System
Su, Wujie ; Wu, Yan ; Wu, Huiling ; Wang, Wenbing ;
International Journal of Industrial Entomology, volume 15, issue 2, 2007, Pages 131~135
Open reading frame 4 of Bombyx mori nucleopolyhedrovirus (BmNPV), designated as Bm4, is a gene whose function is completely unknown. With the recently developed BmNPV bacmid and a modified pFastBac1 whose polyhedrin promoter was replaced with ie1 promoter, a recombinant bacmid expressing Bm4-EGFP fusion protein under the control of ie1 promoter in BmN cells was successfully constructed. The result not only showed that the polyhedrin promoter can be replaced efficiently with other promoters to direct the expression of foreign gene in BmN cells by using Bac-to-Bac/BmNPV baculovirus expression system but also laid the foundation for rescue experiment of Bm4 deletion mutant due to the ability of ie1 promoter to direct gene expression throughout the infection cycle.
Identification and Characterization of Bombyx mori LDH Gene through Bioinformatics Approaches
Zhu, Minfeng ; Chen, Keping ; Yao, Qin ;
International Journal of Industrial Entomology, volume 15, issue 2, 2007, Pages 137~143
Lactate dehydrogenase (LDH) is a ubiquitous enzyme that plays a significant role in the clinical diagnosis of pathologic processes. Discovery of the LDH (BmLDH) gene in B. mori may shed light on its role in the biology of Lepidoptera species, and afford further understanding of the function of the enzyme. In this study, we used the bioinformatics tools to identify LDH gene in B. mori. Sequence analysis showed that BmLDH cDNA contains a 996 bp open reading frame, encoding 331 AA proteins, with seven introns. Compared with hHLDH (human heart LDH), BmLDH contained the same key active sites. Domain search and protein fold recognition analyses provide compelling evidences that the deduced protein is a LDH. Using the computer program MEGA3, we conducted a search for homologs of BmLDH among many eukaryotic species and confirmed that the BmLDH was conserved in all organisms investigated. This gene has been registered in GenBank under the accession number EU000385.
Molecular Markers and Their Application in Mulberry Breeding
Vijayan, Kunjupillai ;
International Journal of Industrial Entomology, volume 15, issue 2, 2007, Pages 145~155
Mulberry (Morus spp.) is an economically important tree crop being cultivated in India, China and other sericulturally important countries for its foliage to feed the silk producing insect Bombyx mori L. Genetic improvements of mulberry lag behind to the same in many other economically less important crops due to the complexity of its genetics, the breeding behavior, and the lack of basic information on factors governing important agronomic traits. In this review, the general usage and advantages of different molecular markers including isoenzymes, RFLPs, RAPDs, ISSRs, SSRs, AFLPs and SNPs are described to enlighten their applicability in mulberry genetic improvement programs. Application of DNA markers in germplasm characterization, construction of genetic linkage maps, QTL identification and in marker-assisted selection was also described along with its present status and future prospects.
Artificial Diet for Mass Rearing the Emma Field Cricket, Teleogryllus emma (Orthoptera: Gryllidae)
Kim, Nam-Jung ; Hong, Seong-Jin ; Seol, Kwang-Youl ; Kim, Seong-Hyun ; Ahn, Nan-Hee ; Park, Hae-Chul ; Lee, Young-Bo ; Kim, Mi-Ae ;
International Journal of Industrial Entomology, volume 15, issue 2, 2007, Pages 157~160
Nymph of the emma field cricket, Teleogryllus emma, were reared on several types of artificial diets. The development period of nymphs were 55.4 days when only a single food, wheat bran, was provided, and it did not show a significant difference compared to the rearing results of the Danong diet and mixed diet. The supplying period of fish meal as the animal feed, the high emergence rates were obtained at 3rd instar with 90% and 4th instar with 100%. For the added amount test, when more than 40% of the diet was added, it confirmed that the insect weight increased. The characteristics of development according to each added amount of the vegetable food (dry bean-curd residue and corn powder) were investigated to minimize the dangers of the degeneration of diet when rearing with a single feed during the
instar period. First, as the added amount of bean-curd residue increased, nymphal development period became longer and the emergence rate became low. With corn powder as the single diet, all died before becoming adult. However, when corn powder was added up to 30%, no difference existed in the breeding results.
Inhibitory Effect of Buthus martensi Karsch Extracts on
Kim, Eun-Ok ; Kim, Shin-Duk ;
International Journal of Industrial Entomology, volume 15, issue 2, 2007, Pages 161~164
While searching for
-glucosidase inhibitors, the active compound was found in a methanol extract of Burthus martensi Kirsch. The separation of the active compound was performed using various chromatography methods and the physico-chemical properties of the purified compound were characterized. The compound showed very potent inhibitory activity against
. Lineweaver-Burk plot indicated that its inhibition of
Changes in the Titer of Protein and Cholesterol Content in Non-Diapause, Artificially Diapause Terminated and Diapause Eggs of Silkworm, Bombyx mori.
Moorthy, S.M. ; Krishnan, N. ; Bhattacharya, Tanmay ; Chaudhuri, A. ;
International Journal of Industrial Entomology, volume 15, issue 2, 2007, Pages 165~169
A differential specific pattern of variation in the metabolism of protein and cholesterol was noticed in non -diapause and diapause eggs due to the significant differences in embryonic development. The rate of metabolism was different due to specific demands of such metabolites during active embryogenesis and maintenance of diapause respectively. In general, the metabolic rate was found to be accelerated in non- diapause eggs just after egg deposition, while it was very slow in diapause eggs. When the diapause eggs were treated with hydrochloric acid within 16-20 hrs, the rate of turnover was found to very similar to non- diapause eggs, though the base level of protein and cholesterol was recorded to be different.
Enhanced Mechanical Property of Silk Sericin Beads Prepared from Ethanol-precipitated Sericin
Oh, Han-Jin ; Lee, Ji-Young ; Lee, Young-Kyu ; Lee, Ki-Hoon ;
International Journal of Industrial Entomology, volume 15, issue 2, 2007, Pages 171~174
Sericin beads were prepared from ethanol-precipitated sericin. The addition of ethanol into hot-water extracted sericin solution induced precipitation of sericin and thereby some sericin could be fractionated. The ethanol-precipitated sericin (EpSS) had narrower molecular weight distribution than original sericin. The EpSS had mainly random coil structure with small portion of
structure. With the EpSS, spherical beads could be prepared at lower concentration than with original sericin due to higher viscosity. The EpSS beads had better compressive strength than the original sericin beads and had rubber-like property. Our results suggest that EpSS is more compatible in the polymeric field, since it has better mechanical strength than original sericin.