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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
International Journal of Industrial Entomology
Journal Basic Information
Journal DOI :
Korean Society of Sericultural Science
Editor in Chief :
Volume & Issues
Volume 17, Issue 2 - Dec 2008
Volume 17, Issue 1 - Sep 2008
Volume 16, Issue 2 - Jun 2008
Volume 16, Issue 1 - Mar 2008
Selecting the target year
Value Addition Span of Silkworm Cocoon - Time for Utility Optimization
Reddy, R. Manohar ;
International Journal of Industrial Entomology, volume 17, issue 1, 2008, Pages 109~113
Realizing the scope of utilizing by-products of silk cocoons by applying appropriate methods is the immediate crave to optimize returns. The nutritive value of pupae suits for human diet, feed for poultry, carps, fish, rabbits, piggery and dogs. The pupal skin, fat, oil, cocoon palade have applications in oleo chemical, soap, glycerin, cosmetic, artificial fibres, membranes and n-triacontanol isolation. The pupal proteins Chitin, Shinki fibroin, Serrapeptidase, glucosamine are latent precursors of post surgical, anti-carcinogenic, anti-inflammative, anti-bacterial, anti-histaminic, gastric, hepatitis, pancreatitis, leukocytopenia, neurological, ophthalmic, blood pressure, cardiac and diabetic medicines and for preparation of vitamins A, E and K. The silk and its proteins sericin and fibroin are potentially used for wound healing, diabetes, impotence, sinusitis, arthritis, edema, cystitis, epididymitis, tissue regeneration, cancer, post-surgical trauma and used as anti-oxidatives, bio-adhesives, ultra violet screens and bio-active textiles. The waste cocoons can be used in making art crafts like garlands, carpets, overcoats, decoratives and greeting cards. The in-depth research towards utility optimization and make aware this reality to sericulturists, reelers, weavers, traders, entrepreneurs, policy makers etc., is the upright want of the today's Sericulture industry.
Development of Model for Efficient Reuse of Abandoned Sericultural Facilities in Korea
Ryu, Kang-Sun ; Kang, Pil-Don ; Jung, I-Yeon ; Kim, Kee-Young ; Lee, Kwang-Gil ; Sohn, Bong-Hee ; Kim, Yong-Soon ;
International Journal of Industrial Entomology, volume 17, issue 1, 2008, Pages 115~120
Recently, there has been the gradual growth of the public in rural amenities, hobby farming works, much more urbanities, and especially near-retires. However, in spite of popular preference to ruralities, little has been known about their demand on retired rural life. Therefore, this study examined urbanites' attitude to preparatory works for out-migration, preferred residential site condition and life styles in the countryside. For the purpose, data were collected from the survey with the sample of 62 urban trainees who were studying farming technology in department of training on agricultural technology of Korea National Agricultural College, RDA. We carried out the experience farming to candidate with using the abandoned sericultural facilities which are the silkworm rearing house and mulberry fields. The 71% of urban trainees, who answered in this survey, hoped to move into countryside after retirement and to make a monthly income of $1,000 from agriculture. The age distribution of sericulural farmers is about 79% more than that of 60 years old. In the experience farming works. The two candidates made 1,977$ with net income through 32 days worked in spring season. In autumn season, the couple candidates made 1,317$ with net income through 23 days worked. Knowledge of these results will be important to policy-makers and to understand the role that retirement in-migration play in stimulating economic growth of rural agricultural farmers.
Synchronized Expression of Two Bombyx mori Caspase Family Genes, ice-2 and ice-5 in Cells Induced by Ultraviolet Irradiation
Wang, Wenbing ; Sun, Ying ; Song, Lina ; Wu, Yan ; Wu, Huiling ;
International Journal of Industrial Entomology, volume 17, issue 1, 2008, Pages 121~124
The caspase family proteins play an important role in programmed cell death (apoptosis). To date, the expression profiles of the caspase family genes in Bombyx mori (Bm) are poorly known. In this study, we examined the expression profiles of two novel Bm caspase family genes (ice-2 and ice-5), the potential change of the mitochondrial membrane and the morphology in Bm cells after stimulation of ultraviolet (UV) irradiation. The results showed the potential change of the mitochondrial membrane occurred at 5 hours after UV irradiation treatment. Analysis of fluorescent quantitative RT-PCR demonstrated that both the ice-2 and ice-5 might be involved in UV induced apoptosis in Bm cells. Notably, after UV irradiating, expression pattern of ice-2 and ice-5 were remarkably different. The ice-2 gene was highly expressed at two time points, 0.5 and 5 hours after UV stimulating, while the expression level of ice-5 only peaked at 5 hours after UV stimulating. It indicated that apoptosis induced by UV irradiation was involved in the mitochondrial pathway and the two isoforms of Bm ice may act but play different role during the apoptosis of Bm cells.
Characterization of Mamestra brassicae Nucleopolyhedrovirus (MabrNPV)-K1 Isolated in Korea
Lee, Jae-Kyung ; Shin, Tae-Young ; Bae, Sung-Min ; Choi, Jae-Bang ; Oh, Jeong-Mi ; Koo, Hyun-Na ; Kim, Ju-Il ; Kwon, Min ; Woo, Soo-Dong ;
International Journal of Industrial Entomology, volume 17, issue 1, 2008, Pages 125~129
The purpose of this study was to investigate the characteristics of Mamestra brassicae nucleopolyhedrovirus (MabrNPV)-K1 isolated in Korea. Polyhedra of MabrNPV-K1 showed irregular appearance in shape with the average diameter
. MabrNPV-K1 contained a number of nucleocapsids within a viral envelope embedded in polyhedron. The polyhedrin of MabrNPV-K1 was composed of single polypeptide with a M.W. of approximate 31 kDa which is identical to the commercialized MabrNPV, Mamestrin, as a biological control agent. The nucleotide and amino acid sequences within the coding region of MabrNPV-K1 polyhedrin shared 99.0% similarity with the polyhedrin gene from previous reported MabrNPVs. The median lethal concentrations (
) of MabrNPV-K1 and Mamestrin to M. brassicae larvae were
PIBs/larva, respectively. Mortality of the MabrNPV-K1 against to the third instars larvae was 15 times higher than that of the Mamestrin. The median lethal times (
of MabrNPV-K1 by the concentration of polyhedra were lower (
days) than those of Mamestrin (
days). These results suggest that a local strain MabrNPV-K1 has high pathogenicity to M. brassicae and may be useful for the development of biological control agent to control this.
Molecular Characterization of a Defensin-like Peptide from Larvae of a Beetle, Protaetia brevitarsis
Hwang, Jae-Sam ; Kang, Bo-Ram ; Kim, Seong-Ryul ; Yun, Eun-Young ; Park, Kwan-Ho ; Jeon, Jae-Pil ; Nam, Sung-Hee ; Suh, Hwa-Jin ; Hong, Mee-Yeon ; Kim, Ik-Soo ;
International Journal of Industrial Entomology, volume 17, issue 1, 2008, Pages 131~135
A cDNA encoding a defensin-like peptide (Protaetiamycine) from the larvae of a beetle, Protaetia brevitarsis was cloned. The DNAs encoded the deduced propeptide of 79 amino acid residues with the predicted molecular weight of 8.4 kDa and PI of 8.24. Overall amino acid sequence of this protein has 39% similarity to that of Rhodnius prolixus defensin, 43% similarity to that of Acalolepta luxuriosa defensin, and 72% similarity to that of Oryctes rhinoceros defensin, suggesting that this gene is an insect defensin. In an attempt to apply the anti-bacterial peptide to the development of therapeutic agents, a 12-mer peptide amidated at its C-terminus, ACAAHCLAIGRG-
, 12Pbn) was synthesized. This peptide showed some antifungal activity against Candida albicans. To increase antifungal activity, six 9-mer peptides were synthesized by modifying amino acid sequences of 12Pbn fragment. Among these peptides, 9Pbm3-9Pbm6 exhibited strong activity compared with Cecropin B and mellitin.
Expression of Active Antibacterial Bumblebee Abaecin in Escherichia coli Cells
Kim, Seong-Ryul ; Hwang, Jae-Sam ; Yoon, Hyung-Joo ; Park, Kwan-Ho ; Hong, Mee-Yeon ; Kim, Kee-Young ; Jin, Byung-Rae ; Kim, Ik-Soo ;
International Journal of Industrial Entomology, volume 17, issue 1, 2008, Pages 137~141
We previously isolated and cloned a cDNA of abaecin from the Bombus ignitus. In an effort to produce a large amount of soluble abaecin at low cost, we successfully expressed the peptide in Escherichia coli that are highly sensitive to its mature form. For this, we fused the peptide encoding 39 amino acids of mature B. ignitus abaecin to the thioredoxin gene together with a C-terminal 6xHis tag. An enterokinase cleavage site was introduced between the 6xHis tag and mature abaecin to allow final release of the recombinant peptide. A high yield of 9.6 mg soluble fusion protein from 200 ml of bacterial culture was purified by
-charged His-Bind resin affinity column, and 1.4 mg of pure active recombinant abaecin was readily obtained by enterokinase cleavage, followed by affinity chromatograph. The molecular mass of recombinant abaecin peptide was determined by Tricin-SDS-PAGE analysis. The recombinant abaecin exhibited antibacterial activity against Gram-negative bacteria.
Molecular Cloning and Characterization of a Muscle-Specific Lipase from the Bumblebee Bombus ignitus
Hu, Zhigang ; Wang, Dong ; Lu, Wei ; Cui, Zheng ; Jia, Jing-Ming ; Yoon, Hyung-Joo ; Sohn, Hung-Dae ; Kim, Doh-Hoon ; Jin, Byung-Rae ;
International Journal of Industrial Entomology, volume 17, issue 1, 2008, Pages 143~151
A muscle-specific lipase gene of the bumblebee Bombus ignitus was cloned and characterized. This gene, which we named Bi-Lipase, consists of seven exons encoding 317 amino acid residues. Bi-Lipase possesses all the features of lipases, including GXSXG consensus motif and Ser-Asp-His catalytic triad. Expressed as a 37-kDa polypeptide in baculovirus-infected insect Sf9 cells, recombinant Bi-Lipase showed an optimal pH of 9.0 and exhibited its highest catalytic activity at
. Furthermore, through the addition of tunicamycin to the recombinant virus-infected Sf9 cells, recombinant Bi-Lipase was found to be N-glycosylated. Northern and western blot analyses indicated that Bi-Lipase was expressed in the wing, thorax, and leg muscles. These results show that Bi-Lipase is a muscle-specific lipase, suggesting a possible role of Bi-Lipase in the utilization of lipids for muscular activity in B. ignitus.
Studies on Tolerance Level Against Antheraea mylitta Cytoplasmic Polyhedrosis Virus (AmCPV) in Different Eco-races of Tropical Tasar Silkworm, Antheraea Mylitta Drury
Singh, G.P. ; Zeya, S.B. ; Srivastava, A.K. ; Ojha, N.G. ; Suryanarayana, N. ;
International Journal of Industrial Entomology, volume 17, issue 1, 2008, Pages 153~155
Pathogenic infection in tasar silkworm, Antheraea mylitta Drury is common and there is a breed specific response regarding tolerance. Hence, the tolerance level of three eco-races of tasar silkworm viz. Daba, Sarihan and Raily to Antheraea mylitta cytoplasmic polyhedrosis virus (AmCPV) infection was tested. The survival of Daba, Sarihan and Raily eco-races was significantly different (p<0.05) when challenged with the same concentration (
polyhedra/ml) of AmCPV. Daba eco-race was more tolerant to the AmCPV infection having higher survival (65.7%) and
values (1000893.17 polyhedra/ml) of AmCPV followed by Sarihan eco-race (50.7% survival and
value of AmCPV 187203.6168 polyhedra/ml) and Raily eco-race (24.3% survival and
value of AmCPV 5176.37 polyhedra/ml). The tolerance level of Daba and Sarihan eco-races against AmCPV was more than two times than Raily eco-race.
Effect of Microsporidian Infection on Reproductive Potentiality on Mulberry Silkworm, Bombyx mori L. (Lepidoptera: Bombycidae) in Different Seasons
Chakrabarti, Satadal ; Manna, Buddhadeb ;
International Journal of Industrial Entomology, volume 17, issue 1, 2008, Pages 157~163
Infection of pathogenic microsporidia, Nosema bombycis and Nosema mylitta (Chakrabarti and Manna, 2006) decreased egg production, fecundity, hatching % and increased sterile eggs in heavily infected mulberry silkmoth, Bombyx mori L. On an average a disease free moth laid upto 442.67 eggs with high hatching % (99.53) and less sterile eggs (
). While an infected moth laid less number of eggs (
) with low hatching % (
) and high sterile eggs (
). Fecundity of disease free laying was highest (468.714) during season-1 then gradually decreased during season- 2 (414.000) to season- 3 (404.285). But fecundity of an infected laying was highest during season-2 and hatched eggs were lowest during season-2. Higher inoculums concentration of N. mylitta infected to 5th stage larva of mulberry silkworm drastically decreased the fecundity in season - 3 and lower inoculums concentration of N. bombycis decreased the fecundity in season-1 and 3. Season-3 was most effective season to decrease the fecundity and increase sterile eggs when both temperature and humidity were fluctuated from the optimum level.