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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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International Journal of Industrial Entomology
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Journal DOI :
Korean Society of Sericultural Science
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Volume & Issues
Volume 17, Issue 2 - Dec 2008
Volume 17, Issue 1 - Sep 2008
Volume 16, Issue 2 - Jun 2008
Volume 16, Issue 1 - Mar 2008
Selecting the target year
Preservation of Acid Treated Bivoltine Eggs in Silkworm Bombyx mori L.
Rajanna, K. L. ; Raju, P.Jayarama ; Prabhakar, C.J. ; Kamble, C.K. ;
International Journal of Industrial Entomology, volume 17, issue 2, 2008, Pages 165~168
The hybrid (
) eggs treated with acid were taken up for the study with an objective to develop long-term preservation schedule. The hybrid eggs obtained with two mating duration (3 h and 6 h) and oviposition period (6 h and 24 h) with two age groups of eggs (24 h and 36 h) were treated with Hydrochloric acid. These eggs were subjected to preservation at
in single step refrigeration and at
under double step refrigeration from
days. These eggs were released from the cold storage as per the specified durations and incubated at standard conditions and allowed 2 h for hatching at 450 lux light. Hatchability was found to be significantly higher or on par with the control in three treatments (T1, T2 and T4) where the eggs are preserved continuously at
up to 30 days. However under double step refrigeration, hatching was not significantly affected in 20+60 day's combination of T1 treatment up to 80 days. Bioassay studies of the promising treatment i.e.. T1 with (20+60) days indicated that early stage loss and cocoon yield was found to be on par with the control. Hence this treatment was recommended for preservation of acid treated new bivoltine hybrid layings. Details of the hatchability and rearing performance of long term preservation of acid treated eggs are discussed.
Ex-situ Stabilization and Utility Prospects of 'Jata' Ecorace of Tropical Tasar Silkworm Antheraea mylitta Drury
Hansda, G. ; Reddy, R. Manohar ; Sinha, M. K. ; Ojha, N. G. ; Prakash, N. B. Vijaya ;
International Journal of Industrial Entomology, volume 17, issue 2, 2008, Pages 169~172
Indian tropical tasar silkworm, Antheraea mylitta D is reported to have forty four ecoraces by way of their adoption to different topographical and vegetational conditions. Of late, another ecorace 'JATA' - univoltine under in-situ condition has been explored which is localized in Thakurmunda area of the foot hills of Simlipal biosphere (Mayurbhanj district) of Orissa, India. The ecorace 'Jata' exhibits superior economic characters over widely commercialized ecoraces of Daba and Sukinda. The cocoon production of localized 'Jata' ecorace is depleting day by day in in-situ condition. In the present study, efforts were made to stabilize the ecorace 'Jata' under ex-situ condition at Ranchi (Jharkhand), where the 'Jata' ecorace silkworms were reared on Terminalia tomentosa for four successive generations during 2006 and 2007 and the cocoons were preserved in grainage house under prevailing climatic conditions. The results indicated change in voltinism behaviour of the 'Jata' ecorace from univoltine to bivoltine. The rearing performance and grainage efficiencies indicate the tendency of 'Jata' ecorace towards acclimatization and stabilization under Ranchi climatic conditions. The 'Jata' ecorace manifests the prospects for acclimatization, stabilization and commercial exploitation.
Influence of Temperature and Relative Humidity in Infection of Nosema bombycis (Microsporidia: Nosematidae) and Cross-infection of N. mylitta on Growth and Development of Mulberry Silkworm, Bombyx mori
Chakrabarti, Satadal ; Manna, Buddhadeb ;
International Journal of Industrial Entomology, volume 17, issue 2, 2008, Pages 173~180
The influence of temperature and relative humidity in infection and cross-infection of Nosema bombycis and N. mylitta respectively in mulberry silkworm, Bombyx mori L. on larval mortality, multiplication of pathogens, larval weight and growth rate in three different seasons were studied. Seasons were selected in such condition, when very less fluctuations between minimum and maximum temperature and minimum and maximum relative humidity (
R.H) was observed i.e., season-1. Fluctuations between minimum and maximum temperature were less (
) but R.H % was more (
) in season-2. Fluctuations between minimum and maximum temperature and R.H % were more (
) in season-3. Growth rate of microsporidian-infected silkworm is directly related to the prevailing temperature and relative humidity in silkworm. Silkworm can tolerate slight variation of temperature but slight variation of relative humidity disfavours the development of silkworm and favours the multiplication of pathogens.
Insecticidal Activity of Paecilomyces fumosoroseus SFP-198 as a Multi-Targeting Biological Control Agent against The Greenhouse Whitefly and The Two-Spotted Spider Mite
Kim, Jae-Su ; Roh, Jong-Yul ; Choi, Jae-Young ; Shin, Sang-Chul ; Jeon, Mun-Jang ; Je, Yeon-Ho ;
International Journal of Industrial Entomology, volume 17, issue 2, 2008, Pages 181~187
An entomopathogenic fungus, Paecilomyces fumosoroceus SFP-198 was isolated in Kyungbuk province, Korea and screened out for the control of Trialeurodes vaporariorum nymphs. It showed 72.5% efficacy against second instars of T. vaporariorum nymphs at 6 days after treatment in the laboratory. To select an active ingredient for the mass production and the formulation, SFP-198 culture products were compared based on their insecticidal activities against T. vaporariorum nymphs. Among them, conidia and blastospores showed much higher insecticidal activity than supernatants. Furthermore, SFP-198 conidia were more heat-resistant than blastospores, and also powder form of conidia was more stable than their suspension form. SFP-198 conidia showed high pathogenicity on not only T. vaporariorum but also Tetranychus urticae in the glasshouse. This result suggested that SFP-198 conidia can be used as a multi-targeting biological control agent against sucking agri- cultural pests, such as whiteflies and mites.
Cloning and Characterization of hydroxypyruvate isomerase (EC 188.8.131.52) gene in silkworm Bombyx mori
Lv, HongGang ; Chen, KePing ; Yao, Qin ; Wang, Lin ;
International Journal of Industrial Entomology, volume 17, issue 2, 2008, Pages 189~195
The sequence of hydroxypyruvate isomerase gene was obtained in NCBI. In this study, the hydroxypyruvate isomerase gene of Bombyx.mori was identified and annotated with bioinformatics tools. The result was confirmed by RT-PCR, prokaryotic expression, mass spectrographic analysis and sub-cellular localization. The hydroxypyruvate isomerase cDNA comtains a 783bp ORF, and has 4 exons. The deduced protein has 260 amino acid residues with the predicted molecular weight of 29169.30 Da, isoelectric point of 6.10, and contains conserved PRK09997 and Hfi domains. The hydroxypyruvate isomerases of Nasonia vitripennis and Bombyx mori have a high homology. Through RTPCR analysis, we found that this transcript was present in testis, ovary, blood-lymph, fat body, midgut, silk gland and tuba Malpighii. This protein was located in cytoplasm through immunohistochemistry. We submitted the cloned gene under the accession number EU344910. The enzyme has been classified under accession number EC 184.108.40.206.
Antioxidant Activity of N-hydroxyethyl Adenosine from Isaria sinclairii
Ahn, Mi-Young ; Heo, Jung-Eun ; Ryu, Jae-Ha ; Jeong, Hy-Kyoung ; Ji, Sang-Deok ; Park, Hae-Chul ; Sim, Ha-Sik ;
International Journal of Industrial Entomology, volume 17, issue 2, 2008, Pages 197~200
The antioxidant activity of Isaria (Paecilomyces) sinclairii was determined by measuring its radical scavenging effect on 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radicals. The n- BuOH extract of P. sinclairii showed strong scavenging activity to DPPH. The anti-oxidant potential of the individual fraction was in the order of ethylacetate> n- BuOH>chloroform>n-hexane. The n-BuOH soluble fraction exhibiting strong anti-oxidant activity was further purified by repeated silica gel column chromatography. N-(2-Hydroxyethyl)adenosine (HEA) was isolated as one of the active principles from the n-BuOH layer. The n-BuOH layer, particularly HEA, did not increase the level of nitric oxide (NO) production in vascular endothelial cells that might be related to vasorelaxation such as the action of viagra. In addition, the vascular endothelial growth factor (VEGF) levels showed little or no increase compared with control group with the treatment of I. sinclairii.
Simple Self Trap Cropping System to Control Tukra Mealy Bug (Maconellicoccus hirsutus Green) Incidence on Mulberry (Morus spp.)
Latha, K. Lavanya ; Rao, T.V.S.S. ; Rao, J. V. Krishna ; Jayaraj, S. ; Reddy, N. Sivarami ; Naik, S. Sankar ;
International Journal of Industrial Entomology, volume 17, issue 2, 2008, Pages 201~203
A field study on self trap cropping system in controlling tukra mealy bug Maconellicoccus hirsutus Green) incidence on mulberry (Morus spp.; V-1 variety;
spacing) gardens was undertaken in selected sericulture farmers' mulberry gardens of Pydeti village (Parigi Mandal, Anantapur District, Andhra Pradesh, India). The study was conducted for three years (2004, 2005 and 2006). Four treatments (T0; control/no treatment, T1; self trap cropping rows with 10 row interval, T2; self trap cropping rows with 10 row interval and treating these rows only with 0.5% neem oil emulsion and T3; self trap cropping rows with 10 rows intervals and treating all the mulberry rows including self trap cropping rows with 0.5% neem oil emulsion) were considered. The results indicated that the incidence increased in T0 and T1 while the same suppressed in T2 and T3. The suppression (%) of tukra incidence between T2 and T3 was not significant. Hence, T2 only was recommended to farmers as its economical viable practice. The results are discussed based on the importance of tukra, its suppression and cost of treatment.
Host Plant-Antheraea mylitta Interactions and Its Effect on Reproductive and Commercial Parameters
Rath, S.S. ; Singh, G.S. ; Singh, S.S. ; Singh, M.K. ; Suryanarayana, N. ; Vijayaprakash, N.B. ;
International Journal of Industrial Entomology, volume 17, issue 2, 2008, Pages 205~209
Impact of food plant on reproductive and commercial parameters in Antheraea mylitta, a polyphagous insect of economic importance was studied upon feeding the insect larvae on the same host plants for six continuous generations. A. mylitta larvae were fed upon Terminalia tomentosa, Terminalia arjuna and Zizyphus jujuba and restricted them to the same host plant for six generations to document the quantitative improvement in reproductive and commercial parameters. The parameters showed significant improvement in all the host plants studied over their respective controls. Fecundity among the reproductive parameters was highly improved than others (85.9% in T. tomentosa; 58% in T. arjuna and 49.7% in Z. jujuba). Likewise in commercial parameters, the shell weight in male showed the highest improvement (by 52.9%, 45.8% and 42.1% in T. tomentosa; T. arjuna and Z. jujuba respectively). On the other hand, the shell ratio percentage in female recorded the lowest improvement. The values for all characters were recorded a decline in T. arjuna and Z. jujuba fed ones over T. tomentosa, except that of shell ratio percentage in female has registered an increase in Z jujuba fed. The study thus revealed the comparative superiority of T. tomentosa over T. arjuna and Z. jujuba.
Identification of an Entomopathogenic Fungus, Beauveria bassiana SFB-205 toxic to the Green Peach Aphid, Myzus persicae
Kim, Jae-Su ; Roh, Jong-Yul ; Choi, Jae-Young ; Shin, Sang-Chul ; Jeon, Mun-Jang ; Je, Yeon-Ho ;
International Journal of Industrial Entomology, volume 17, issue 2, 2008, Pages 211~215
To select entomopathogenic fungi controlling aphids effectively, several isolates were screened against second instars of Myzus persicae nymphs in the glasshouse using conidia suspension at
conidia/ml. Among these isolates, SFB-205 conidia showed the highest insecticidal activity about 32.7% efficacy to M. persicae at 4 days after application in the glasshouse. The attachment of SFB-205 conidia on the surface of M. persicae nymphs, and germination and penetration were observed using scanning electron microscopy. SFB-205 was identified as Beauveria bassiana species through the comparison of 5.8 s rRNA genes. There were 24 polymorphisms between SFB-205 and the previously reported isolate, B. bassiana ATCC74040 using six kinds of primer combinations in amplified fragment length polymorphism (AFLP) analysis. The B. bassiana SFB-205 might be used as a practical biological control agent for the green peach aphid, M. persicae in the field.
Proteomic Assessment of Dung Beetle, Copris tripartitus Immune Response
Suh, Hwa-Jin ; Bang, Hea-Son ; Kim, Seong-Ryul ; Yun, Eun-Young ; Park, Kwan-Ho ; Kang, Bo-Ram ; Kim, Ik-Soo ; Jeon, Jae-Pil ; Hwang, Jae-Sam ;
International Journal of Industrial Entomology, volume 17, issue 2, 2008, Pages 217~221
Dung beetle larvae at the
instar were injected with lipopolysaccaride and inducible proteins were examined within a pI level of 3-10 and a size level by proteomics, including 1-D SDS PAGE analysis and antibacterial assay. The immune infected larvae extracts provided seven protein bands in one-dimensional electrophoresis and its antibacterial activity also checked. Hemolymph protein from immune infected larvae of the dung beetle were separated by twodimensional gel electrophoresis and compared with those from native larvae. In 2-D gel electrophoresis, we detected 63 immune infected unique and 32 up-regulated proteins, and 36 proteins that were down-regulated or not present in treated gel. Ten protein spots from unique proteins and those presented as different level of abundance in infected and native larvae were specially expressed. These differentially expressed proteins were proposed to be involved in the defense mechanism against microorganism.
Identification of An Antibacterial Gene by Differential Display from Lipopolysaccharide-Stimulated Dung Beetle, Copris tripartitus
Suh, Hwa-Jin ; Kim, Yeon-Ju ; Bang, Hea-Son ; Yun, Eun-Young ; Kim, Seong-Ryul ; Park, Kwan-Ho ; Kang, Bo-Ram ; Kim, Ik-Soo ; Jeon, Jae-Pil ; Hwang, Jae-Sam ;
International Journal of Industrial Entomology, volume 17, issue 2, 2008, Pages 223~228
A novel beetle antimicrobial protein from stimulated Copris tripartitus and the corresponding gene were isolated in parallel through differential display-PCR and expression in Escherichia coli. To find cDNA clones responsible for bacteria resistance, the suppression subtractive hybridization and GeneFishing differentially expressed genes system were employed in the dung beetle, Copris tripartitus immunized with lipopolysaccaride. One cDNA clone from eight subtracted clones was selected through dot blot analysis and confirmed by northern blot analysis. The 516-bp, selected cDNA clone was determined by 5' and 3' rapid amplication of cDNA ends and cloned into the GST fusion expression vector pGEX-4T-1 for expression of the protein. The expressed protein was predicted 14.7 kDa and inhibited the growth of gram-negative bacteria such as Escherichia coli and Pseudomonas aeruginosa. These results implied that the expressed protein is related to immune defense mechanism against microorganism.
Comparative Study of Gene Expression Profiles in Posterior Silk Glands of the Silkworm, Bombyx mori L.
Choi, Kwang-Ho ; Goo, Tae-Won ; Kang, Seok-Woo ; Kang, Min-Uk ; Yun, Eun-Young ; Hwang, Jae-Sam ;
International Journal of Industrial Entomology, volume 17, issue 2, 2008, Pages 229~234
We used serial analysis of gene expression (SAGE) approach to derive a profile of expressed genes of the posterior silk glands (PSG) and to create a reference for understanding gene cluster related to the mechanism of silk protein synthesis in the silkworm, Bombyx mori. We constructed a 3' SAGE library from the PSG of the fifth instar larvae of the silkworm. In total we obtained 2,406 SAGE tags, of which 682 were unique tags. Sorted by tag count number, 27 (4%) unique tags were significantly more abundant genes (ten or more times), whereas 445 (65%) unique tags were detected as single copies. The annotation of 682 unique SAGE tags revealed that 462 (68%) of the SAGE tag sequences represented known genes, whereas 220 (32%) of the tag sequences had no matches in SAGE map and silkworm EST databases. Of the 682 SAGE tags, the most abundant tag sequences were that of the fibroin light chain gene and the silk protein P25. In addition, we compared two relative abundance results of the SAGE and the EST approaches to verify whether their transcript quantitative aspects are significant or not. The comparative results of relative abundances of the fibroin H-, L- chain and P25 glycoprotein genes indicated that the quantitative approach based on SAGE tags is effective for quantitative cataloging and comparison of expressed genes in same organs. The SAGE tag information reported in this study would be useful for researchers in the field to analyze genes associated with silk processing mechanisms of insects.