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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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International Journal of Industrial Entomology
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Journal DOI :
Korean Society of Sericultural Science
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Volume & Issues
Volume 19, Issue 2 - Dec 2009
Volume 19, Issue 1 - Sep 2009
Volume 18, Issue 2 - Jun 2009
Volume 18, Issue 1 - Mar 2009
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Single Nucleotide Polymorphisms (SNPs) for Advanced Genomic Research in Sericulture
Vijayan, Kunjupillai ;
International Journal of Industrial Entomology, volume 19, issue 1, 2009, Pages 143~154
Single nucleotide polymorphisms (SNPs) are the most frequent form of variation in the genome of any organism. Owing to their greater abundance, they are considered useful for identifying cultivars, construction of higher density linkage maps, and detection of genes (QTLs) associated with complex agronomic traits and diseases. Although, SNPs have been used recently for constructing a high density genetic map in silkworm and a set of 118 SNPs have been identified in tasar silkworms, not much progress has been made in sericulture to utilize the vast potential of SNPs. Thus, this review mainly focuses on some of the important methods of SNP discovery, validation and genotyping. Emphasis has also been given to the possible uses of SNP genotyping in the improvement of silkworms and their host plants.
Insect Adaptations to Changing Environments - Temperature and Humidity
Singh, Tribhuwan ; Bhat, Madan Mohan ; Khan, Mohammad Ashraf ;
International Journal of Industrial Entomology, volume 19, issue 1, 2009, Pages 155~164
The most important factors in environment that influence the physiology of insects are temperature and humidity. Insects display a remarkable range of adaptations to changing environments and maintain their internal temperature (thermoregulation) and water content within tolerable limits, despite wide fluctuations in their surroundings. Adaptation is a complex and dynamic state that widely differs in species. Surviving under changing environment in insects depends on dispersal, habitat selection, habitat modification, relationship with ice and water, resistance to cold, diapause and developmental rate, sensitivity to environmental signals and syntheses of variety of cryoprotectant molecules. The mulberry silkworm (Bombyx mori) is very delicate and sensitive to environmental fluctuations and unable to survive naturally because of their domestication since ancient times. Thus, the adaptability to environmental conditions in the silkworm is quite different from those of wild insects. Temperature, humidity, air circulation, gases and photoperiod etc. shows a significant interaction in their effect on the physiology of silkworm depending upon the combination of factors and developmental stage affecting growth, development, productivity and quality of silk. An attempt has been made in this article to briefly discuss adaptation in insects with special emphasis on the role of environmental factors and their fluctuations and its significance in the physiology of mulberry silkworm, B. mori.
Growth and Foliar Constituents of Mulberry (
) Cultivated under Organic Based Nutrient Management
Rashmi, Krishnegowda ; Shankar, Maruvanahalli Ankegowda ; Shashidhar, Kaluvarahalli Ramanna ; Narayanaswamy, Talagavara Kempaiah ;
International Journal of Industrial Entomology, volume 19, issue 1, 2009, Pages 165~169
A field experiment to evaluate the effect of application of different organic manures and inorganic fertilizers on growth, yield and quality of leaf was studied during 2004-05 has showed that, the application of 10 kg each of Azospirillum brasilense and Aspergillus awamori+20% each of recommended N through compost+green manure (Glyricidia maculata)+castor cake+vermicompost+Urea and remaining P and K through fertilizers (
) has recorded significantly higher leaf yield (250 g/ plant and 34.70 tonnes/ha/yr, respectively) with improvement in growth characters as compared to control. Leaf quality status was also improved in terms of N (3.19%), P (1.97%), K (1.28%), total soluble protein (8.39 mg/ml), total soluble sugars (14.40 mg/ml), secondary nutrients viz., Ca (3.00%), Mg (0.60%), S (0.35%) and micronutrients viz., Cu (0.410 ppm), Mn (0.454 ppm) and Zn (0.112 ppm) contents. The mulberry grown with 20 tonnes of compost+300:120:120 Kg of NPK/ha/year through fertilizer ranked second for growth and foliar constituents.
Potential Usage of Food Waste as a Natural Fertilizer after Digestion by Hermetia illucens (Diptera: Stratiomyidae)
Choi, Young-Cheol ; Choi, Ji-Young ; Kim, Jong-Gill ; Kim, Myung-Sook ; Kim, Won-Tae ; Park, Kwan-Ho ; Bae, Sung-Woo ; Jeong, Gil-Sang ;
International Journal of Industrial Entomology, volume 19, issue 1, 2009, Pages 171~174
The black soldier fly larvae are able to decompose various organic wastes such as livestock manures and food wastes. We tested whether the quality of the insect derived compost, i.e. larval feces, was comparable to that of a commercial fertilizer. The results show that the chemical composition and the growth rate of cabbages grown on the insect derived compost are virtually identical to those on the commercial fertilizer. Therefore the insect derived compost will be an ideal substitute to commercial fertilizers.
Single Oral Dose Toxicity Study of an Alcohol Extract of Bombus ignitus pupae in Rats
Ahn, Mi-Young ; Han, Jea-Woong ; Yoon, Hyung-Ju ; Hwang, Jae-Sam ; Park, Hae-Chul ; Seo, Yun-Jung ; Chung, Wan-Tae ;
International Journal of Industrial Entomology, volume 19, issue 1, 2009, Pages 175~180
Recently, as the male silkworm pupae, bee pupae have the potential that strengths men's vitality on vascular endothelial nitric oxide in endothelial cells. Especially we prepared alcohol extract of pupae of bumblebee, native bee named Hobakbul, Bombus ignitus. The alcohol extract of pupae of B. ignitus was administered to rats at doses of 0, 0.04, 0.2, 1 or 2 g/kg as a single oral dose. There were no observed clinical signs or deaths related to treatment in all the groups tested. Therefore, the approximate lethal dose of the alcohol extract B. ignitus pupae was considered to be higher than 2 g/kg in rats. Mild decreases in body weight gain in male were observed dose-dependently within B. ignitus pupae alcohol extract treated groups in dose response manner over 2 weeks. Throughout the administration periods, no significant changes in diet consumption, ophthalmologic findings, clinical pathology (hematology, clinical chemistry and coagulation) or gross pathology were detected. Minor changes in male and female rats were found in hematological parameters for all or partial of B. ignitus pupae extract treated groups but all the changes observed were within the physiological range. From these results, it was concluded that there was no-evidence of specific toxicity related to the ingestion of alcohol extract of B. ignitus pupae.
The Effect of HPMC Concentration on the Morphology and Post Drawing of Wet Spun Regenerated SF/HPMC Blend Filaments
Ko, Jae-Sang ; Um, In-Chul ;
International Journal of Industrial Entomology, volume 19, issue 1, 2009, Pages 181~185
In this paper, the regenerated silk fibroin (SF)/hydroxypropyl methylcellulose (HPMC) blend filaments were prepared by wet spinning and the effect of HPMC concentration on the post drawing and morphology of blend filaments was elucidated. The result of maximum draw ratio indicated that the wet spinnability of wet spun SF / HPMC was improved with increasing HPMC concentration until 8% and remained constant after that concentration. The SEM observation revealed that the enhanced wet spinnability of blend filaments was strongly related to the morphological change by increasing HPMC concentration. Regardless of HPMC concentration, as SF content was reduced, the wet spinnability of blend film decreased resulting in reduced maximum draw ratio. It was also found by SEM observation that the cross section of blend filament deviated from circularity with an increase of HPMC content.
Silk Filament Progression with Backcross Breeding Generations in Tropical Tasar Silkworm, Antheraea mylitta D
Reddy, Rangareddygari Manohar ; Suryanarayana, Nagabathula ; Sinha, Manoj Kumar ; Gahlot, Nand Singh ; Hansda, Ganga ; Ojha, Nand Gopal ; Prakash, Nanjappa Basappa Vijaya ;
International Journal of Industrial Entomology, volume 19, issue 1, 2009, Pages 187~192
Silk filament progression study applying backcross breeding with recipient parent Jata and donor parent Daba ecoraces of tropical tasar silkworm, Antheraea mylitta Drury conducted during 2006 to 2008, revealed introgression of filament denier (10.2 d) superior to both parents at BC4 level. Also, the silk waste (0.35%) and filament breaks (2.6 nos) were reduced compared to both parents, while the filament length (973 m) and non break filament (296 m) improved over donor and could attain closer to recipient parent. The best performance of Jata
Daba at F1 level, with highest silk filament length (1646 m) of 12.5 d denier, denotes heterosis impact on silk trait with parental heterogenousity, an advantage to exploit silk filament yield. The progression of quality in terms of finer filament denier, less silk waste and least number of filament breaks over both parents and improvement in filament length and non break filament over donor parent except for a marginal shortage against recipient at BC4 level indicates the prospects of Jata and Daba ecoraces as source of breeding material for qualitative improvement of tasar silk filament. The study suggests that the commercially important finer denier of tasar silk filament can be attained with minor reduction in silk yield of wild Jata ecorace by adopting repeated backcrossing for four generations with semi domesticated Daba ecorace.
Effect of Propolis Volatiles from a Stingless Honeybee(Apidae : Meliponinae) on the Immune System of Elderly Residents in a Nursing Home
Suzuki, Seiko ; Amano, Kazuhiro ; Suzuki, Koichi ;
International Journal of Industrial Entomology, volume 19, issue 1, 2009, Pages 193~197
We report an evaluation of the immunostimulatory effect of propolis volatiles from a stingless honeybee. We studied 34 elderly residents at a special nursing home. Twenty-one subjects were treated with propolis, 8 with
charcoal and 5 subjects acted as controls. Subjects treated with either propolis or Bincho charcoal were housed in rooms separated from the other non-study residents in the nursing home. The effects of each treatment on natural killer (NK) cell activity and lymphocyte levels were examined after 2 months and then for a longer period. The results indicated that NK cell activity was significantly improved to that within the normal range only after propolis treatment.
Construction of Modified Bacillus thuringiensis cry1Ac Genes for Transgenic Crop Through Multi Site-directed Mutagenesis
Xu, Hong Guang ; Roh, Jong-Yul ; Wang, Yong ; Choi, Jae-Young ; Shim, Hee-Jin ; Liu, Qin ; Tao, Xueying ; Woo, Soo-Dong ; Jin, Byung-Rae ; Je, Yeon-Ho ;
International Journal of Industrial Entomology, volume 19, issue 1, 2009, Pages 199~204
The newly cloned Bacillus thuringiensis cry1-5 gene showed high activity to both Plutella xylostella and Spodoptera exigua, while cry1Ac only showed high activity against P. xylostella but low to S. exigua. Through the alignment of amino acid sequences between Cry1Ac and Cry1-5, we found 12 different residues in domain I (6 residues) and domain II (6 residues). In this study, the modified cry1Ac gene, which is constructed according to a crop-preferring codon usage, was used as a template to construct mutant B. thuringiensis cry1Ac genes based on cry1-5 gene through multi site-directed mutagenesis. Total 63 various mutant cry genes were obtained at 12 positions randomly. Among them, ten mutant cry genes, whose domain I was totally converted and domain II was randomly, were selected to express in baculovirus expression system as a polyhedrin fusion form. The recombinant proteins were 95 kDa in size and were stably activated as 65 kDa by trypsin. The expressed mutant Cry proteins were applied to bioassays against P. xylostella and S. exigua. All mutants showed high insecticidal activity both to P. xylostella and S. exigua similar to cry1-5. These results suggest that these mutant cry genes might be expected of desirable cry genes for introduction to transgenic crops.
Character Analysis of Silkworm Strains Registered as Genetic Stocks in Korea
Kang, Pil-Don ; Kim, Kee-Young ; Jung, I-Yoen ; Kim, Mi-Ja ; Jin, Byung-Rae ; Kim, Ik-Soo ;
International Journal of Industrial Entomology, volume 19, issue 1, 2009, Pages 205~213
In order for further systematic maintenance of silkworm stocks kept in Korea we analyzed character quality of a diverse array of silkworm strains originated from several sericulture-practicing countries. The analysis of about ten qualitative characters from 67 strains (13 of Japanese strains, 15 of Chinese strains, 14 of European strains, 6 of Korean and Tropical strains, and 19 of unknown origin) revealed a significant difference in the ten different qualitative characters among silkworm strains. In the analysis of quantitative characters, Japanese and European strains were highest in hatchability, the Korean and Tropical strains were highest in pupation rate, and unknown origin and Chinese strains were highest in cocoon yield and number of egg laid. With the connection of molecular genetic analysis the current data may provide the advanced ground for further systematic maintenance of valuable genetic resources of silkworms, although more breeds should be investigated for further complete pictures.