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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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International Journal of Industrial Entomology
Journal Basic Information
Journal DOI :
Korean Society of Sericultural Science
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Volume & Issues
Volume 19, Issue 2 - Dec 2009
Volume 19, Issue 1 - Sep 2009
Volume 18, Issue 2 - Jun 2009
Volume 18, Issue 1 - Mar 2009
Selecting the target year
Effect of Mating Duration and Multiple Use of Male Moth on Reproductive Performance of Some Cross Breeds of Silkworm, Bombyx mori L.
Sarkar, Kunal ; Mandal, Manmatha ; Moorthy, Shanmugam Manthira ;
International Journal of Industrial Entomology, volume 19, issue 2, 2009, Pages 215~219
The effect of mating duration and multiple use of male on fecundity and fertility was investigated in the hybrids of Silkworm, Bombyx mori L. One hour to five hours of mating duration did not affect egg laying (fecundity) significantly, while fertilization affected significantly. Below 50% fertility was noticed when mating duration was reduced to below two hours. Similarly multiple use of male caused significant reduction in fertility but not fecundity. Till fourth mating no significant reduction in fertility was observed, thereafter fertility reduced considerably.
Impact of Nosema sp. Infection on Nutritional Physiology and Growth of the Tasar Silkworm Antheraea mylitta
Rath, Sudhansu Sekhar ; Singh, Mrinal Kanti ; Suryanarayana, N ;
International Journal of Industrial Entomology, volume 19, issue 2, 2009, Pages 221~228
Nosema sp. infection in the Indian tasar silkworm, Antheraea mylitta exerts a complex of influences on its host. The instar duration was extended significantly (P<0.001) except in
instar. The infected larvae took about 48 days to reach the spinning stage against 40 days in the uninfected ones. The final weight attained by the larva at the end of each instar of development declined significantly following infection, as did weight gain and relative growth rate (RGR). The growth recorded/ day declined in infected larvae compared to uninfected ones from 8.2% during
instar to 43.3% during
instar. Food ingestion and digestion increased with advancement of the instar significantly irrespective of the status of the larvae but the relative consumption rate (RCR) declined. These parameters significantly declined in infected larvae (except food digested during
instar). The decline was more during
instar. In contrast, the approximate digestibility (AD %) was significantly higher in infected larvae than uninfected ones leaving the
instar larvae unaffected. The efficiency of conversion of ingested food (ECI) and efficiency of conversion of digested food (ECD) did not change in a patterned way following the microsporidia (Nosema sp.) infection. The values of ECI significantly changed during
instars; while the change in ECD during
were significant. During the entire larval life all the parameters tends to decline significantly following microsporidia infection but AD registered a significant increase. Nosema sp. spore concentration has increased 270.7 times during larval development in the course of experimentation.
Molecular Cloning and Characterization of a Lipocalin in the Bumblebee Bombus Ignitus
Hu, Zhigang ; Yoon, Hyung-Joo ; Sohn, Hung-Dae ; Jin, Byung-Rae ;
International Journal of Industrial Entomology, volume 19, issue 2, 2009, Pages 229~235
We have cloned and characterized a lipocalin from the bumblebee Bombus ignitus (Bi-lipocalin). The Bi-lipocalin gene spans 2284 bp and consists of four exons coding for 270 amino acid residues. Sequence analysis revealed that Bi-lipocalin possesses three structurally conserved regions (SCTs) that characterize lipocalins. Recombinant Bi-lipocalin, expressed as a 37 kDa protein in baculovirus-infected insect cells, was N-glycosylated, indicating that the carbohydrate moieties are necessary for secretion. Tissue distribution analysis revealed ubiquitous expression of Bi-lipocalin in all tissues examined. Bi-lipocalin transcripts were upregulated by stress, such as wounding,
exposure, and external temperature shock. These results indicate that Bi-lipocalin is a stress-inducible protein that acts on wounding,
overexposure and temperature stimulation.
Pathogenicity and Production of Mamestra brassicae Nucleopolyhedrovirus (MabrNPV)-K1
Choi, Jae-Bang ; Lee, Jae-Kyung ; Bae, Sung-Min ; Shin, Tae-Young ; Koo, Hyun-Na ; Kim, Ju-Il ; Kwon, Min ; Woo, Soo-Dong ;
International Journal of Industrial Entomology, volume 19, issue 2, 2009, Pages 237~241
The objective of our study was the evaluation of pathogenicity of a local strain of Mamestra brassicae nucleopolyhedrovirus-K1 (MabrNPV-K1) derived from a diseased larva of M. brassicae found in Korea. The effect of temperature and larval instar on the pathogenicity and production of MabrNPV-K1 was determined under laboratory conditions. The median lethal concentration (
) values of MabrNPV-K1 for 3rd instar larvae were
, respectively. The
for the 4th instar larvae was similar to that for the 3rd instar larvae. However, the pathogenicity to the 3rd instar larvae was higher than that to the 4th instar larvae. The median lethal time (
) values of MabrNPV-K1 were 11.4 to 5.0 days at
and 18.3 to 5.5 days at
for the 3rd instar larvae. The
value was lowered as temperature went up to
and dependent on viral concentration. In production efficiency of MabrNPV-K1 using M. brassicae larvae, the mortality of the 3rd instar larvae was 100% when inoculated with
PIBs/larva and the yield of MabrNPV-K1 was maximal. Regarding the mortality, yield of polyhedra, inoculation doses and required time, the
was determined as optimal conditions producing polyhedra efficiently.
Studies on Determination of Larval Critical Weight in Silkworm, Bombyx mori L. - An Index of Quality
Saha, Atul Kumar ; Chaudhuri, Anath Bandhu ; Moorthy, S Monthira ; Roy, Subrata ;
International Journal of Industrial Entomology, volume 19, issue 2, 2009, Pages 243~248
The Larval critical weight is the minimal mass at which further growth in not necessary for a normal time course to pupation. Larval critical weight (Lcw), pupal critical weight (Pcw) and adult critical weight (Acw) of different breeds of Bombyx mori L. were determined through starvation (food deprivation). The Lcw was found to be about 938.46, 2397.26, 2283.57 and 2220.97 mg in males and 1118.15, 2681.04, 2604.9 and 2455.88 mg in females of the multivoltine breed (Nistari), Bivoltine breeds (P5 & NB18) and their hybrid (P5
NB18) respectively. Bivoltine breed P5 took more time (3.35 days) followed by NB18 (3.13 days) & P5
NB18 (3.02 days) to attain larval critical weight (In
Instar) than the multivoltine breed Nistari (2.42 days). Decrease in weight from larval maximal weight to pupal weight and to adult weight was also observed more in multivoltine than bivoltines, which may be due to more latent feeding period in bivoltines. Since Lcw is a stable character and independent of environment, it could be utilized for characterization of silkworm breeds to assess the quality of an insect.
Assessment of Genetic Diversity in Different Populations of Raily Ecorace of Indian Tasar Silkworm, Antheraea Mylitta Drury Using ISSR Markers
Srivastava, Ashok Kumar ; Kar, Prasanta Kumar ; Sinha, Ravibhushan ; Sinha, Manoj Kumar ; Vijayaprakash, Nanjappa Basavappa ;
International Journal of Industrial Entomology, volume 19, issue 2, 2009, Pages 249~253
Raily ecorace of Indian tasar silkworm is wild in nature and distributed abundantly in dense deciduous forest on Shorea robusta (Sal) in Bastar (
E and altitude ranging from 150 to 1200 mMSL) forest ranges of Chhattisgarh, India. It is represented by about 20 populations. Out of those, eleven populations showed intra- as well as inter- population variability based on phenotypic expression and also in major economic traits viz. cocoon weight, shell weight, filament length and denier. Genetic diversity in these eleven populations was studied using Inter-Simple Sequence Repeat (ISSR) markers. The band profiles generated with eight ISSR primers have depicted variation in band size. All the primers exhibited polymorphism which is an indicative of the genetic variation in individual Raily silkworm. Among the populations, total polymorphism recorded was 76%. The population genetic aspects assessed through POPGENE software package are discussed in the paper. Nei's gene diversity (h) ranged from 0.194 to 0.337 exhibiting high heterozygosity. Relevance of the present study is of high significance in formulating conservation strategies and sustainable utilization of the economically important Raily ecorace of Antheraea mylitta.
Sericinjam Sericin: Structural and Thermal Properties
Kim, Jong-Ho ; Shin, Bong-Seob ; Lee, Kwang-Gill ; Yeo, Joo-Hong ; Kweon, Hae-Yong ;
International Journal of Industrial Entomology, volume 19, issue 2, 2009, Pages 255~258
Structure and Properties of sericinjam sericin extracted was investigated using SDS-PAGE electrophoresis, UV, CD, and DSC. The molecular weight and its distribution of sericinjam sericin showed broad tailing pattern. Circular dichlroism spectra showed that the structure of sericinjam sericin in solution was different with domestic one. However, differential thermal calorimetric properties are similar to each other.
Enhanced Resistance to Botrytis cinerea Mediated by Transgenic Expression of the Spider Chitinase Gene AvChit in Arabidopsis
Hur, Yeon-Jae ; Kim, Doh-Hoon ;
International Journal of Industrial Entomology, volume 19, issue 2, 2009, Pages 259~264
The AvChit gene encodes for a chitinase from the spider, Araneus ventricosus. This spider, A. ventricosus, is an abundant species in Korea. Arabidopsis thaliana plants were transformed with the AvChit gene using Agrobacterium tumefaciens. Thirteen transgenic lines expressing the AvChit gene were obtained. Functional expression of the AvChit gene in transgenic Arabidopsis was confirmed by Southern, northern and western blot analysis. The AvChit cDNA was expressed as a 61 kDa polypeptide in baculovirus-infected insect Sf9 cells. AvChit protein extracted from transgenic Arabidopsis exhibited high levels of chitinase activity. Phytopathological tests showed that two transgenic Arabidopsis lines expressing the AvChit gene displayed high levels of resistance to gray mold disease (Botrytis cinerea).
Effect of Parasitoids' Exit and Predators' Ingress Holes on Silk Yield of the African Wild Silkmoth, Gonometa Postica Walker (Lepidoptera: Lasiocampidae)
Fening, Ken Okwae ; Kioko, Esther Ndaisi ; Raina, Suresh Kumar ;
International Journal of Industrial Entomology, volume 19, issue 2, 2009, Pages 265~268
Wild silkmoths can be utilised sustainably in the production of silk as an income for resource-poor rural communities. However, attack by parasitoids and predators affect the quality of cocoons and quantity of raw silk produced. A laboratory experiment was undertaken to quantify the effect of parasitoids' (dipteran and hymenopteran) and predators' (ants) exit and ingress holes, respectively, on silk production. The mean number of shells required to produce fifty grams of raw silk was highest with cocoons parasitised by a dipteran and lowest with unattacked cocoons (but with moths already emerged). Degumming loss was highest in parasitised and lowest in unttacked cocoons, but both were not different from cocoons predated by ants. Shell weight was highest in unattacked cocoons, followed by hymenopteran-parasitised and predated cocoons, with the dipteran parasitized ones being the least. Single cocoon weight was greater in hymenopteran-parasitised and predated cocoons than the dipteran-parasitised and unattacked cocoons. Shell ratio or raw silk, floss and yarn weights were higher in unattacked than parasitised and predated cocoons. The total loss in raw silk attributable to attack by parasitoids and predators ranged between 17.4~31.2%. The results offer baseline information for assessment of economic losses in wild silk farming due to parasitoids and predators in the field.