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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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International Journal of Industrial Entomology
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Journal DOI :
Korean Society of Sericultural Science
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Volume & Issues
Volume 3, Issue 2 - Dec 2001
Volume 3, Issue 1 - Sep 2001
Volume 2, Issue 2 - Jun 2001
Volume 2, Issue 1 - Mar 2001
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Comparative Analysis of Completely Sequenced Insect Mitochondrial Genomes
Lee, Jin-Sung ; Kim, Ki-Hwan ; Suh, Dong-Sang ; Park, Jae-Heung ; Suh, Ji-Yoeun ; Chung, Kyu-Hoi ; Hwang, Jae-Sam ;
International Journal of Industrial Entomology, volume 2, issue 1, 2001, Pages 1~6
This paper reports a few characteristics of seven insect mitochondrial genomes sequenced completely (Bombyx mori, Drosophila melanogaster, D. yakuba, Apis mellifera, Anopheles gambiae, A. quadrimaculatus, and Locusta migratoria). Comparative analysis of complete mt genome sequences from several species revealed a number of interesting features (base composition, gene content, A+T-rich region, and gene arrangement, etc) of insect mitochondrial genome. The properties revealed by our work shed new light on the organization and evolution of the insect mitochondrial genome and more importantly open up the way to clearly aimed experimental studies for understanding critical roles of the regulatory mechanisms (transcription and translation) in mitochondrial gene expression.
A Comparison of Silk Fibroin Hydrolysates by Hydrochlonic Acis and Proteolytic Enzymes
Sh. R. Madyarov ; Yeo, Joo-Hong ; Lee, Kwang-Gill ; Lee, Yong-Woo ;
International Journal of Industrial Entomology, volume 2, issue 1, 2001, Pages 7~13
Enzymatic hydrolysis of different forms of silk fibroin (soluble, gel and insoluble forms) by industrial and commercial enzyme preparations to obtain aqueous and powdered silk fibroin in relatively mild conditions was investigated. A mono-enzymatic hydrolysate systems were tested for hydrolysis of water-soluble form of fibroin as most productive form of protein substrate. Insoluble forms of substrate usually were hydrolyzed less effective. In some cases from soluble fibroin substrate gel was formed during hydrolysis process. This hindered intermixing and decreased rates of hydrolysis. Insoluble sediments were formed in enzymatic hydrolysates in other cases. These sediments and also sediment after chemical hydrolysis were purified and tested on amino acids content for comparison. Sediments formation in these conditions are considered as pure tyrosine isolation method. Obtained hydrolysates were characterized by gel-chromatography analysis and other standard biochemical methods. Possibility of application of enzymatic hydrolysis for preparation of silk fibroin hydrolysates is discussed.
Effect of Silk Fibroin on the Protection of Alcoholic Hepatotoxicity in the Liver of Alcohol Preference Mouse
Kang, Gyung-Don ; Lee, Ki-Hoon ; Do, Sun-Gil ; Kim, Chung-Sub ; Suh, Jun-Gyo ; Oh, Yang-Seok ; Nahm, Joong-Hee ;
International Journal of Industrial Entomology, volume 2, issue 1, 2001, Pages 15~18
Silk fibroin (SF) derided from the domestic silk worm, bombyx mori, is the natural protein and widely used as bio-functional materials as well as apparels. We studied the livers protective effect of SF from alcohol-induced hepatotoxicity in the alcohol preference mouse. To increase more absorption of SF in experimental animals, molecular weight of SF was lowered by 2N of HCI aqueous solution at 10
for 48 hrs. SF was added to liquid diet with alcohol and fed to the alcohol preference mice for 4 weeks. To assess the liver function, the concentration of alanine aminotransferase (AlT), aspartate aminotransferase (AST) and cholesterol present in either blood or liver tissue were measured. As compared with non-SF treated groups the SF-treated showed significantly low concentrations of ALT, AST, cholesterol and triacylglycerol values, respectively. Histopathological examination revealed that the extent of hepatocyte injury in the SF-treated group was reduced when it was compared with non SF-treated group. These results suggest that SF may have liver protective effects against alcohol-induced hepatotoxicity.
Construction and Characterization of Transformed Insect Cells Expressing Baculovirus Very Late Factor in an Infection-Independent Manner
Park, Hye-Jin ; Lee, Kwang-Sik ; Cho, Eun-Sook ; Yun, Eun-Young ; Kang, Seok-Woo ; Kim, Keun-Young ; Sohn, Hung-Dae ; Jin, Byung-Rae ;
International Journal of Industrial Entomology, volume 2, issue 1, 2001, Pages 19~26
Transformed Spodoptera frugiperda (Sf9) cells expressing baculovirus very late factor (VLF-1) were constructed by using Autograha nuclear polyhedrosis virus (AcNPV) immediate earthy gene (ie1). Neomycin-resistance gene as a selectable marker was introduced under the control of AcNPV ie1 promoter, and Bombyx mori nuclear polyhedrosis (BmNPV-K1) vlf-1 gene was introduced under the control of the Drosophila heat shock protein gene (hspr70) promoter to yield dual expression plasmid with two independent transcription units. It was transfected into Sf9 cells and cell clones expressing vlf-1 were selected by G4l8 treatment. Genomic DNA from transformed cells was isolated and integration of AcNPV iel harboring vlf-1 was confirmed by PCR using AcNPV iel-specific primers and Southern blot analysis. The transformed cells expressing VLF-1 in an infection-independent manner expressed foreign gene product of recombinant baculovirus in the earlier stage of infection compared with control Sf9 cells. These results suggest the possible to develop highly efficient transformed insect cells for baculovirus expression vector system.
Variation of Mineral Compositions in the Regional, Varietal, and Seasonal Mulberry Leaves
Kim, Iksoo ; Lee, Heui-Sam ; Kim, Jin-Won ; Yang, Bo-Kyung ; Ahn, Mi-Young ; Kim, Doh-Hoon ; Ryu, Kang-Sun ;
International Journal of Industrial Entomology, volume 2, issue 1, 2001, Pages 27~35
This study was carried out to investigate the mineral content in the regional, varietal and seasonal mulberry leaves. On average, mulberry leaf samples contained minerals in the order of potassiumi phosphorus, calcium, magnesium, iron and so on (2.494 g/100 g, 2.255 g/100 g, 1.835 g/100 g, 0.627 g/100 g, 0.0245 g/100 g DW, respectively). Calcium content was 19-fold and 4-fold higher than that of green tea and spinach, respectively, suggesting that mulberry loaves appear to be a calcium-rich food source. In the comparison of geographic samples of Cheongilppong variety, calcium bevel was highest in Youngchun sample (2.477 g/100 g) and highest potassium level in Suwon sample (2.962 g/100 g). In the geographic samples of YK209 variety, Jinju-City sample was highest in calcium content (1.509 g/100 g). Among wild mulberry loaves collected from Cheju Island and Tsushima, potassium level was highest in Bongge-dong, Cheiu City (3.865 g/100 g) and calcium level in Mitshshima Town, Tsushima (2.948 g/100 g). In the comparison of varietal samples collected in Suwon at the mulberry field of Dept. of Sericulture & entomology, Shinkwangppong variety was highest in the potassium levels although Keryangppong and Shinkwangppong were higher in calcium revel. In the comparison of seasonal samples of Cheongilppong, there was a rough trend of increase in some minerals up to July (e.g., calcium rind )potassium). Finding of the highest calcium and potassium contents in the wild mulberry (3.865 g/100 g and 2.948 g/100 g, respectively) rather than in the cultivated ones warrant that more focus should be paid to wild mulberry leaves to utilize their minerals effciently.
A Phylogenetic Study in Some Long-Horned Beetles (Coleoptera: Cerambycidae) Using Mitochondrial COI Gene and 16S rRNA Sequences
Yoon, Hyung-Joo ; Bae, Jin-Sik ; Kim, Iksoo ; Jin, Byung-Rae ; Mah, Young-Il ; Moon, Jae-Yu ; Sohn, Hung-Dae ;
International Journal of Industrial Entomology, volume 2, issue 1, 2001, Pages 37~53
Two regions of mtDNA genome, cytochrome oxidase subunit I (COI) and 165 ribosomal RNA (165 rRNA) genes, were sequenced for 15 species of the long-horned beetle belonging to four subfamilies and geographic samples of mulberry longicorn beetle, Apriona germari, from two localities in Korea. Ten samples of A. germari collected from Suwon and Busan revealed three COI haplotypes ranging in nucleotide divergence of 0.3% to 0.5%, and the two populations shared one common COI haplotype (80%). The sequence divergence among 15 species of the long-horned beetle was much higher in COI gene (12.3%∼39.4%) than 16S rRNA gene (7.2% to 23.1), and the maximum value in the COI gene is exceptional compared with other relevant studies, including that of Coleoptera. The greatly increased divergence in the COI gene, in facto was stemmed from a peculiar sequence of Prionus insularis belonging to Prioninne, divergence of which ranges from 31.2% to 39.3% from other species. We discussed possible reason of the divergence in this species. Due to the abnormality of COI gene divergence, decrease in phylogenetic signal was severe in COI nucleotide and, subsequently, the converted amino acid sequences, rendering us to put more confidence on the 16S5 rRNA gene data. Although the molecular phylogeny confidently supports the monophyletic origin of Lepturinae, the presence of discrepancy between molecular data and traditional taxonomic views also is a testable hyothesis. One such discrepancy includes taxonomic position of Sophronica obrioides and Theophilea cylindricollis belonging to Lamiinae.
Purification and Characterization of Vitellin from the Red Flour Beetle, Tribolium castaneum Herbst
Kim, Seong-Ryul ; Choo, Young-Moo ; Lee, Seong-Jin ; Jin, Byung-Rae ; Kim, Jeong-Ho ; Heo, In-Bum ; Shon, Hung-Dae ;
International Journal of Industrial Entomology, volume 2, issue 1, 2001, Pages 55~59
The vitellin of the red flour beetled Tribolium castaneum Herbst was purified and characterized. The vitellin of T. castaneum was purified by the FPLC techniques, anion exchange chromatography and gel permeation chromatography. In native-polyacrylamide gel electrophoresis, vitellin of T. castaneum was detected as a single band. This native vitellin has molecular weight of 440 kDa. The vitellin of T. castaneum is composed of three polypeptides, designated Vnl (178 kDa), Vn2 (168 kDa) and Vn3 (52 kDa) in SDS-polyacrylamide gel electrophoresis. Three subunits of vitellin were presented in the female adult hemolymph and egg extracts, but not observed in the male. These three polypeptides gradually decreased during embryogenesis. Polyclonal antiserum raised against purified vitellin reacted with the three polypeptides, Vnl, Vn2 and Vn3. Antisera raised against Vn1 and Vn2 cross-reacted with the two large subunits, Vnl and Vn2, respectively. Another subunits Vn3, however, was not cross-reacted with these two antisera. Also, antiserum raised against Vn3 did not cross-react with the Vn1 and Vn2.
Molecular Cloning of the Bombyx Ubiquitin Holmologue Gene That Is Up-regulated Upon Infection
Yun, Eun-Young ; Goo, Tae-Won ; Hwang, Jae-Sam ; Kang, Seok-Woo ; Park, Soo-Jung ; Kwon, O-Yu ;
International Journal of Industrial Entomology, volume 2, issue 1, 2001, Pages 61~64
Ubiquitin can be covalently attached to cellular proteins as a post-translational modification rind is involved in metabolic stresses, such as bent shock and immune response. We have isolated and sequenced a cDNA encoding ubiquitin from the silkworm, Bombyx mori. The insert in the clone is 533 nucleotide long with an open reading frame of 387 nucleotides that encodes a protein of 129 amino acids with a molecular weight of 14.8 kDa. The amino acid sequence shared high homology with the ubiquitins known so far, The result of dot blot hybridization showed that the B. mori ubiquitin gene is up-regulated upon f. rofi infection, suggesting that the B. mori ubiquitin plays an immune-related role.
Breeding ova New Silkworm Variety, Chunsujam, with a High Silk Yielding for Spring Rearing Season
Kang, Pil-Don ; Kim, Kye-Myeong ; Sohn, Bong-Hee ; Lee, Sang-Uk ; Woo, Soon-Ok ; Hong, Sung-Jin ;
International Journal of Industrial Entomology, volume 2, issue 1, 2001, Pages 65~68
A new silkworm variety, Chunsujam, for spring rearing season is F
hybrid between Japanese race Jam145 bred by 8459/8711 and Chinese race Jam146 by 8544/M8626. Jam145, Japanese parent of the Chunsujam, showed high GCA in pupation rate and Jam146, Chinese parent, showed high GCA in pupation rate and single cocoon weight. In the local adaptability test performed at 8 local areas in spring of 1999, Chunsujam was 5% higher in larval weight, 3% in single cocoon weight, and 4% in cocoon yield from 10,000 of the 3rd molted larvae, respectively, than the check variety Baegokjam.
Effect of Androstenedione on the Economic Parameters of the Silkworm, Bombyx mori
Goudar, K.S. ; Kaliwal, B.B. ;
International Journal of Industrial Entomology, volume 2, issue 1, 2001, Pages 69~74
The effect of topical application with androstenedione on economic parameters was analyzed following treatment of last larval stadium. The treated larvae showed significantly increased larval weight along with other enhanced larval, cocoon and adult parameters. The larval period was significantly shortened in all the treated groups with increased cocooning percentages cocoon weight and its shell weight in female and filament length, weight and denier in all the treated groups. Hatching percentage increased significantly in dose dependent manner in all the treated groups when compared with the corresponding parameters of the carrier control group. This suggests that androstenedione can be used effectively for commercial silkworm rearing.
Yield Loss in Mulberry Due to Sucking Pest Whitefly, Dialeuropora decempuncta Quaintance and Baker (Homoptera: Aleyrodidae)
Bandyopadhyay, U.K. ; Kumar, M.V.Santha ; Das, K.K. ; Saratchandra, B. ;
International Journal of Industrial Entomology, volume 2, issue 1, 2001, Pages 75~78
The whitefly, Dialeuropora decempuncta Quaintance and Baker (Homoptera : Aleyrodidae) causing considerable damage to mulberry, Morus alba. Crop loss caused by the whitefly was estimated in mulberry. Quantitative yield loss was estimated on the basis of harvest data from both sprayed and unsprayed plots. Data on pest incidence has been recorded at weekly intervals in both sprayed and unsprayed plots from 30 th day of plant age till harvesting i.e., 60 days after pruning in August October season. An attempt has been made to establish a relationship between whitefly population and percentage of crop loss due to it. Percentage of crop loss due to whitefly has got a linear relationship with the whitefly population. An initial population of 24 adults/top leaf would be able to cause 24% loss (l,630 Kg leaf/ha ) in a period of 30 days. Economic analysis postulates that application of pesticide can stove a net amount of 1,630 Kg leaf/ha which is sufficient to produce 67.65 Kg of additional multivoltine cocoons. This determines the cost/benefit ratio which will enable to workout the economics of management practices.
Studies on the Degree of Genetic Divergence for Different Quantitative Traits Between Paremntal Lines of Silkworm, Bombyx mori L., Hybrids
Petkov, Naoum ; Grekov, Dimitar ; Ramnali, Paraskevi ;
International Journal of Industrial Entomology, volume 2, issue 1, 2001, Pages 79~81
A study was conducted to establish the degree of genetic divergence between different hybrid forms and rearing conditions through estimation of the minimum number of genes (allelic pairs) differentiating parents in terms of specific quantitative traits. It was established that the minimum gene numbers differentiating parental lines in the inheritance of cocoon was 1, of cocoon shell weight- between 1 and 2, and of silk filament length- between 2 and 3. The variability in the specific genetic parameter could be explained by the reliability of the statistical-and-genetic method used and the expression of genes affecting the formation of each of the characters tested. Gene expression, in its turns is conditioned both by the gene interaction within the genotypes and the different genotype response to environmental change. To go deep in the problem, experiments should be conducted under strictly controlled conditions, reducing the mathematical-and-genetic analysis to a physiological levels and hence to analyse the genetic nature of the specific quantitative character formation and its genetic control.
Effects of Photoperiods on the Growth of the Entomopathogenic Fungi, Paecilomyces japonica, During the Production of the Silkworm-dongchunghacho, Silkworm Vegetable Wasp and Plant Worm
Lee, Eun-Ha ; Park, Nam-Sook ; Park, Sang-Bong ; Lee, Ho-Oung ; Jang, Chang-Sic ; Jin, Byung-Rae ; Lee, Sang-Mong ;
International Journal of Industrial Entomology, volume 2, issue 1, 2001, Pages 83~86
Effects of photoperiods, 24L or 24D, on the growth of the silkworm-dongchunghacho, the silkworm vegetable wasp and plant worm, were investigated. Exposure of the fungi under the photoperiod of 24L for at least 3 days during the cultivation of the fungi after the completion of endosclerotium in the host accelerated the spore formations but the growth of the fruiting bodies was inhibited. On the contrary, the photoperiod of 24D inhibited the spore formation, but accelerated the growth of fruiting bodies without spores. Accordingly, to produce silkworm vegetable wasp and plant worm of large-size fruiting bodies with over 3 cm in length, it is indicated that recommendable light condition is a photoperiod of 24D during the cultivation until the length of the fruiting body arrives at over 3 cm.
Cloning, Sequencing and Characterization of Mitochondrial Control Region of the Domestic Silkwom, Bombyx mori
Lee, Jin-Sung ; Kim, Ki-Hwan ; Hoe, Hyang-Sook ; Park, Jae-Heung ; Kang, Seok-Woo ; Lee, Sang-Han ; Hwang, Jae-Sam ;
International Journal of Industrial Entomology, volume 2, issue 1, 2001, Pages 87~89
The nucleotide sequence of the domestic silkworm (Bombyx mori) mitochondrial (mt) control region and its flanking genes was determined from PCR clones. The control region of the silkworm mt genome was located between the small ribosomal RNA gene and transfer RN
. This 499 bp control region hale 95.4% A+T content. Extensive comparative analysis studies performed with similar control region of other insect genomes could not reveal a highly conserved region containing conserved motifs of animal mito-chondrial genome. The remarkable feature that found in this control region was the presence of tandem motifs containing nine repetitive sequences. The potential usefulness of this motif sequences for Bombyx species or their taxonomically related species is enhanced by its unique localization in the maternally inheritance mitochondrial molecule.e.