Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
International Journal of Industrial Entomology
Journal Basic Information
Journal DOI :
Korean Society of Sericultural Science
Editor in Chief :
Volume & Issues
Volume 21, Issue 2 - Dec 2010
Volume 21, Issue 1 - Sep 2010
Volume 20, Issue 2 - Jun 2010
Volume 20, Issue 1 - Mar 2010
Selecting the target year
Prospects of Application of Linkage Disequilibrium Mapping for Crop Improvement in Wild Silkworm (Antheraea mylitta Drury)
Vijayan, Kunjupillai ; Singh, Ravindra Nath ; Saratchandra, Beera ;
International Journal of Industrial Entomology, volume 20, issue 2, 2010, Pages 37~43
The wild silkworm, Antheraea mylitta Drury (Lepidoptera: Saturniidae) is a polyphagous silk producing insect that feeds on Terminalia arjuna, T. tomentosa and Shorea robusta and is distributed in the forest belts in different states of India. Phenotypically distinct populations of the A. mylitta are called "eco-race" or "ecotypes". Genetic improvement of this wild silkworm has not progressed much due to lack of adequate information on the factors that control the expression of most of the economically important traits. Considering the amazing technological advances taking place in molecular biology, it is envisaged that it is now possible to take greater control on these intractable traits if a combination of genetic, molecular and bioinformatics tools are used. Linkage disequilibrium (LD) mapping is one such approach that has extensively been used in both animal and plant system to identify quantitative trait loci (QTLs) for a number of economically important traits. LD mapping has a number of advantages over conventional biparental linkage mapping. Therefore, LD mapping is considered more efficient for gene discovery to meet the challenge of connecting sequence diversity with heritable phenotypic differences. However, care must be taken to avoid detection of spurious associations which may occur due to population structure and variety interrelationships. In this review, we discuss how LD mapping is suitable for the dissection of complex traits in wild silkworms (Antheraea mylitta).
On the Breeding of Dumbbell Bivoltine Silkworm Breeds of Bombyx mori L. Tolerant to High Temperature and High Humidity Conditions of the Tropics
Singh, Harjeet ; Kumar, Nair Suresh ;
International Journal of Industrial Entomology, volume 20, issue 2, 2010, Pages 45~59
It is well established fact that under tropical condition, unlike polyvoltines, bivoltines are more vulnerable to various stresses i.e. hot climatic conditions of tropics, poor leaf quality and improper management during summer which are not conducive for bivoltine rearing. Therefore, attempt has been made in this study to develop promising bivoltine breeds tolerant to high temperature and high humidity conditions of the tropics. In the present study, by utilizing temperature tolerant breeds six breeding lines were made and at every generation the 5th instar larvae were exposed to high temperature and high humidity and the survived ones were back crossed with the breeds moderately tolerant to diseases were made to improve the quantitative traits. From F6 generations, alternate rearing in normal temperature and high temperature were conducted. At the end of F12 generation, it was possible to isolate three dumbbell breeds viz., HH8, HH10 and HH12 with improvement in quantitative traits. The methodologies followed for the development are discussed.
The Impact of Hybrid Vigour on Commercial Traits of the Indian Tropical Tasar Silkworm, Antheraea mylitta Drury (Lepidoptera: Saturniidae)
Reddy, Rangareddygari Manohar ; Suryanarayana, Nagabathula ; Rai, Suresh ; Sinha, Manoj Kumar ; Hansda, Ganga ; Ojha, Nand Gopal ; Prasad, Bhagwan Chandra ;
International Journal of Industrial Entomology, volume 20, issue 2, 2010, Pages 61~67
The bivoltine tropical tasar ecoraces of Antheraea mylitta Drury produces cocoons for seed production under seed crop rearing (July-August with
temperature and 50~70% relative humidity) and for silk production under commercial crop rearing (September-December with
temperature and 60~80% relative humidity). To consider the impact of hybrid vigour on egg, larva, cocoon and silk related commercial traits, the
hybrids made among Daba, Jata and Raily ecoraces were assessed successively for three years. The hybrid vigour in the
hybrid of Daba
) was positive for egg fertility (+23.1%), shell weight (+25.6%), silk yield (+79.0%) and filament length (+68.1%), with filament of high denier (11.98d) and reduced larval span (-7.1%). The Jata
) hybrid has shown negative heterosis in egg fertility (-8.0%) compared to the other F1 hybrids, Daba
) and Raily
), when they rise simultaneously during the commercial crop season. The better performance of parental ecoraces (
) in their commercial traits during commercial crop over parents of seed crop (
) and the superior performance of
) over parents of commercial crop (
) during commercial crop season indicates the apparent hybrid vigour in tasar
hybrids. As the Daba
) and Jata
hybrids have shown highest hybrid vigour, their rearing during commercial crop can optimize the silk productivity and commercial sustenance of the tasar silk industry.
Impact of Quantitative Feeding on Nutritional Parameters of Fifth Instar Larvae of Antheraea mylitta
Rath, Sudhansu Sekhar ;
International Journal of Industrial Entomology, volume 20, issue 2, 2010, Pages 69~74
Feeding is an important aspect of insect nutrition which in turn supports growth, development, reproduction and survival. The impact of quantitative feeding on nutritional parameters of fifth instar Antheraea mylitta larvae was studied by providing fresh leaves of Terminalia tomentosa for 1 to 4 times a day. All the parameters improved upon providing fresh diets more times over the single diet (1FD). The absolute body weight increased with increase in number of feeds/day significantly (P<0.001). While the maximum weight recorded was 29.61 g in 1FD, the respective weights for two feeds/day (2FD), three feeds/day (3FD) and four feeds/day (4FD) were 39.41 g, 40.63 g and 42.66 g respectively. Feeding period and instar duration were declined (P<0.001) and survival increased significantly (P<0.001) upon increase in the number of diets. Nutritional indices like ingestion, digestion, relative consumption rate (RCR), relative growth rate (RGR) and gain in body weight increased significantly with the increase in number of feeds/day, but approximate digestibility (AD) and efficiency of conversion of ingested food (ECI) declined, while efficiency of conversion of digested food (ECD) did not change.
Testosterone Secretion Effect according to the Growth Stage of Silkworm (Bombyx mori L.)
Ryu, Kang-Sun ; Lee, Heui-Sam ; Kang, Pil-Don ; Kim, Kee-Young ; Kim, Mi-Ja ; Lee, Kwang-Gill ;
International Journal of Industrial Entomology, volume 20, issue 2, 2010, Pages 75~77
Until now, the main treatment for impotence has been the vasodilator injection, penile implants and etc. Among many methods, most effective way is known to be the medical therapy. The oral drug for an impotence remedy has to be above all, effective to the erection, and second, safety when taking in long-term. However, so far the drug in such condition and sufficiency has not been discovered. Consequently, it is crucial to develop the new medicine, made of natural materials only, without any uncertainty of side effect. The silkworm is very difference in physiological chemical change according to growth stage. Therefore the functional effect shows a very big difference according to preparation method with a growth stage. This research carry out the experiment with the pupa powder immediately before the eclosion, eclosion adult, the pupa extract immediately before the eclosion, and the silkworm powder of 5 instar 3rd day. The result showed not a big chance in weight, feed and water intake quantity. But testosterone hormone secretion effects of the freeze drying powder of the pupa immediately before the eclosion was very high 7.31 ng/ml, compared to 2.37 ng/ml of non treatment and 2.67 ng/ml of alcohol extraction method. As this result, the freeze drying pupa powder of the pupa immediately before the eclosion was confirmed to promote the secretion of the testosterone hormone more than alcohol extraction method of existing.
Evaluation of General and Specific Combining Ability in Newly Synthesized Inbred Lines of Bivoltine Silkworm Bombyx mori L.
Goel, Ajay Kumar ; Chandrashekharaiah, Chandrashekharaiah ; Rao, Y. Uma Maheshwar ;
International Journal of Industrial Entomology, volume 20, issue 2, 2010, Pages 79~85
Eight bivoltine inbred lines of silkworm Bombyx mori L. viz., AP8, AP10, AP17 and AP18 with marked female and plain male larvae and AP1, AP54, AP71 and AP72 with all plain larvae synthesized from Chinese commercial hybrids were evaluated for their general combining ability (gca), specific combining ability (sca) and reciprocal effects for eight quantitative traits. The line AP18 was found to be best general combiner by virtue of significant positive and positive gca effects for seven out of eight traits studied followed by the lines AP10, AP8 and AP17. Seven hybrids recorded significant positive and positive sca effects for all the traits under study reflecting the interaction of alleles, additive and non additive gene action and dominant effects expressed by genes. Among the 28 reciprocal hybrids, AP54
AP18 and AP1
AP17 revealed significant reciprocal effects for the six traits followed by AP54
AP8 for five and AP10
AP71 for four traits.
PEGylation of Silk Fibroin Model Peptide
Kweon, Hae-Yong ; Jo, You-Young ; Yeo, Joo-Hong ; Woo, Soon-Ok ; Han, Sang-Mi ; Lee, Kwang-Gill ;
International Journal of Industrial Entomology, volume 20, issue 2, 2010, Pages 87~91
Silk fibroin model peptide, alanine pentamer was synthesized through solid-phase method and modified with poly(ethylene glycol). Nuclear magnetic resonance spectrometry and Fourier-transform infrared spectroscopy showed the conformation of alanine pentamer,
-sheet structure and random coil conformation were not changed with PEGylation. Differential scanning calorimetry showed that relatively strong exothermic peak around
by PEGylation. No cytotoxicity of PEGylated pentamer was observed by L929 cell proliferation test.
Chilling Temperature and Humidity to Break Diapause of the Bumblebee Queen Bombus terrestris
Yoon, Hyung-Joo ; Lee, Kyeong-Yong ; Hwang, Jae-Sam ; Park, In-Gyun ;
International Journal of Industrial Entomology, volume 20, issue 2, 2010, Pages 93~98
Bumblebees are widely used to pollinate various crops, especially tomato, in greenhouses and fields. An artificial hibernation is essential for year-round rearing of the bumblebee, which passes through one generation per year. Here, we investigated whether a chilling temperature and humidity affect artificial hibernation of the bumblebee queen Bombus terrestris. In chilling temperature regimes of
under constant humidity >70%, the queens stored at
exhibited the highest rate of survival, which was 74.0% at one month, 67.0% at two months, 60.0% at three months, 46.0% at 4 months, 33.0% at 5 months, and 24.0% at 6 months. Rates of survival decreased at the following temperatures:
. Colony developmental characteristics after diapause were 1.2- to 1.5-fold higher than those of queens stored at
. In terms of chilling humidity, the queens hibernated at 70% under
exhibited the highest rate of survival, which was
at one month,
at two months,
at 3 months and
at 5 months. The rates of oviposition, colony foundation and progeny-queen production of queens hibernated at 70% were 80.8%, 30.8% and 30.8%, respectively. These values correspond to 1.7- to 3.3-fold increases in comparison to queens stored at 50% humidity. Therefore,
and 70% R.H. were the favorable chilling temperature and humidity conditions for diapause break of B. terrestris queens.
The Effect of Dissolution Condition on the Yield, Molecular Weight, and Wet- and Electro-spinnability of Regenerated Silk Fibroins Prepared by LiBr Aqueous Solution
Cho, Hee-Jung ; Um, In-Chul ;
International Journal of Industrial Entomology, volume 20, issue 2, 2010, Pages 99~105
In this paper, the regenerated silk fibroins were dissolved in LiBr aqueous solution with different dissolution temperature and time, and the effects of the dissolution condition on the regeneration yield, molecular weight, wet spinnability, and electrospinnability of regenerated silk fibroin were investigated. The regeneration yield, molecular weight distribution, and wet spinnability of regenerated silk fibroin were nearly affected by the dissolution temperature and time. However, the electrospinning performance of silk fibroin was influenced by the dissolution condition implying the electrospinning of silk fibroin is more sensitive process than the wet spinning in the range tested in this study. While
of dissolution temperature resulted in a good electrospinnability of regenerated silk fibroin, the electrospinnability was slightly deteriorated when silk fibroin was dissolved at
for 6 hours. Also, though the fiber diameters of electrospun silk fibroin produced by the dissolution at
for 6 hours and 24 hours were 443 and 451 nm, respectively, that at
for 5 min was reduced to 411 nm. The fiber diameter was more decreased to 393 nm when the dissolution time increased up to 6 hours at
Utilization of the Bombyx mori Hypothetical Protein 32 Promoter for Efficient Transgene Expression
Goo, Tae-Won ; Kim, Sung-Wan ; Kim, Seong-Ryul ; Park, Seung-Won ; Kang, Seok-Woo ; Lee, Kwang-Gill ; Kwon, O-Yu ; Yun, Eun-Young ;
International Journal of Industrial Entomology, volume 20, issue 2, 2010, Pages 107~114
For stable germline transformation, the promoter of Bombyx mori cytoplasmic actin gene (BmA3) has been used for ubiquitous expression of transgenes. So far, no strong promoter is available for ubiquitous expression in B. mori, excluding BmA3 promoter. To identify more powerful promoter than previously reported BmA3 promoter, we isolated 9 clones that show stronger signal compared to BmA3 by a dot blot hybridization. Among these 9 clones, we focused on one clone which has high amino acid homology (85%) with hypothetical protein 32 gene of Lonomia obliqua. This clone, named bHp32 (B. mori hypothetical protein 32) was ubiquitously expressed in all tissues and developmental stage of fifth instar B. mori larvae. As result of promoter assay using dual luciferase assay system, we found the highest transcription activity region (-1,200/+220) in the 5'-flanking region of bHp32 gene, which has 42-fold more intensive promoter activity than BmA3 promoter. Moreover, the bHp32 promoter was normally regulated in Bm5, Sf9, and S2 cells. Therefore, we suggest that bHp32 promoter may be used more powerful and effectively for transgene expression in various insects containing B. mori as a universal promoter.
Breeding of Two New Silkworm Varieties with Peculiar Laval Mark, "Eolrukmal" and "Hukpyobeom"
Kang, Pil-Don ; Jung, I-Yeon ; Kim, Kee-Young ; Kim, Mi-Ja ; Sohn, Bong-Hee ; Lee, Gwang-Gill ;
International Journal of Industrial Entomology, volume 20, issue 2, 2010, Pages 115~116
The Eolrukmal and Hukpyobeom varieties were bred for special purpose such as experience and education. The Eolrukmal variety was selected and succeeded from the
Galwon in 2004 summer. It has stripes and brown half-moon body marks. The Hukpyobeom variety was selected and succeeded from the
Hukho in 2004 summer. It has black body color and bright yellow segments. They are healthy yellow cocoon spining varieties showing over 93% of high practical hatching ratio and 93.1% of emergence ratio. The Eolrukmal moth laid 450 egg/batch and the Hukpyobeom moth laid 440 egg/batch.