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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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International Journal of Industrial Entomology
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Journal DOI :
Korean Society of Sericultural Science
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Volume & Issues
Volume 21, Issue 2 - Dec 2010
Volume 21, Issue 1 - Sep 2010
Volume 20, Issue 2 - Jun 2010
Volume 20, Issue 1 - Mar 2010
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Expression of Recombinant Human Stem Cell Factor (hSCF) Protein using Bombyx mori Protein Disulfide Isomerase (bPDI)
Kim, Sung-Wan ; Yun, Eun-Young ; Kim, Seong-Ryul ; Park, Seung-Won ; Kang, Seok-Woo ; Lee, Kwang-Gill ; Kwon, O-Yu ; Goo, Tae-Won ;
International Journal of Industrial Entomology, volume 21, issue 2, 2010, Pages 151~155
Protein disulfide isomerase (PDI) catalyzes the oxidation of disulfides and the isomerizatiob of incorrect disulfides in new polypeptides during folding in the oxidizing environment of the endoplasmic reticulum (ER). To increase recombinant protein hSCF (human stem cell factor) production, we have developed expression system using the Bombyx mori PDI (bPDI) as a fusion partner. bPDI gene fusion was found to improve the production of recombinant hSCFs. Thus, we conclude that bPDI gene fusion will be very useful for the large-scale production of biologically active recombinant proteins.
Characterization of the Open Reading Frame 35 of Bombyx mori Nucleopolyhedrovirus
Zhu, Ying Min ; Li, Guo Hui ; Yao, Qin ; Chen, Ke Ping ; Guo, Zhong Jian ;
International Journal of Industrial Entomology, volume 21, issue 2, 2010, Pages 157~162
Open reading frame 35 (bm35) of the Bombyx mori nucleopolyhedrovirus (BmNPV) is a special gene whose homologues are only found in some group-I nucleopolyhedroviruses, suggesting that bm35 plays a specific role in the viral life cycle. This paper described the characterization of BmNPV bm35. Computerassisted sequence analysis shows that a putative RING finger motif is observed in the protein, Bm35 encoded by bm35. The coding sequence of bm35 was amplified and subcloned into the vector pET30a(+) and the
-tagged fusion protein His-Bm35 was expressed in the Escherichia coli BL21 (DE3) LysS cells. The bm35 transcript and Bm35 protein were detected in BmNPV-infected BmN cells at 12~48 h post infection (p.i.) by RT-PCR and Western blot analysis using the polyclonal antibody generated by immunizing a rabbit with purified
-tagged Bm35, suggesting that bm35 is synthesized in the late stage of BmNPV infection cycle. Bm35 was not a structural component associated with budded virus (BV) and occlusion derived virus (ODV). These data indicated that bm35 is a functional gene in the BmNPV life cycle.
Comparative Analysis of
-glucosidase Activity in Bombyx mori and Antheraea yamamai
Kang, Kyung-Don ; Kamita, Shizuo George ; Suzuki, Koichi ; Seong, Su-Il ;
International Journal of Industrial Entomology, volume 21, issue 2, 2010, Pages 163~167
]Glucosidase (EC 22.214.171.124) is a glycosidase that hydrolyzes disaccharides, oligosaccharides, and polysaccharides resulting in the release of α-D-glucose. In this study,
-glucosidase activity in the hemolymph and midgut of the mulberry silkworm Bombyx mori and Japanese oak silkmoth Antheraea yamamai was measured using maltose, sucrose, trehalose, and p-nitrophenyl
-D-glucopyranoside as substrates. In general, hemolymph
-glucosidase activity was higher in B. mori than in A. yamamai. In contrast, midgut
-glucosidase activity was higher in A. yamamai than in B. mori for all of the substrates tested.
-Glucosidase activity in the midgut of both B. mori and A. yamamai showed similar responses to changes in pH and temperature for all of the substrates tested. Native (7.5%) PAGE of hemolymph and midgut proteins from B. mori and A. yamamai followed by staining with 4-methylumbelliferyl
-D-glucoside (MUG) indicated that the
-glucosidases of these related lepidopterans are functionally similar but structurally different. In comparison to
-glucosidase activity from A. yamamai,
-glucosidase activity from B. mori was generally less sensitive to the
-glucosidase inhibitors, 1-deoxynojirimycin (DNJ), acarbose, and voglibose when the activity was determined using maltose, sucrose, and trehalose.
Anti-adipogenic Effect of Hydrolysate Silk Fibroin in 3T3-L1 Cells
Chon, Jeong-Woo ; Lee, Kwang-Gill ; Park, Yoo-Kyoung ; Park, Kyung-Ho ; Yeo, Joo-Hong ;
International Journal of Industrial Entomology, volume 21, issue 2, 2010, Pages 169~174
Hydrolysate silk fibroin (HSF) is a fibrous protein composed of parallel
-structures and is made from pure silk elements including 18 amino acids, with glycine, alanine, and serine comprising of over 80% of the amino acids. Numerous studies have documented a range of effects of HSF, including moisturizing, antioxidant activity, nervous system disorders, and many more. We investigated whether HSF has anti-obesity effects in vitro. The effects of HSF inhibition on lipid accumulation and acceleration of lipid degradation in 3T3-L1 cells were studied. Treatment of 3T3-L1 cells with HSF caused significant inhibition of cell viability, an increase in glycerol release, and a decreased in adipocyte differentiation. Moreover HSF stimulated downregulated of adipogenic enzyme expressions (PPAR
) and up-regulated of fatty oxidation enzyme expressions (CPT-1 and UCP-2). Based on these results, hydrolysate silk fibroin can be suggested as a potential therapeutic substance as part of a prevention or treatment strategy for obesity.
Silk Fibroin Membrane as Guided Bone Regeneration in Rat Calvarial Defects
Kweon, Hae-Yong ; Kim, Seong-Gon ; An, Jin-Hee ; Shim, Hye-Won ; Yang, Byoung-Eun ; Kim, Jwa-Young ; Jo, You-Young ; Yeo, Joo-Hong ; Lee, Kwang-Gill ;
International Journal of Industrial Entomology, volume 21, issue 2, 2010, Pages 175~179
Silk fibroin membrane was prepared and examined to know the feasibility of SF membrane as guided bone regeneration. The morphology of silk membrane was flat and smooth surface. The conformation of silk fibroin was
-sheet structure. When the silk membrane was applied on the rat calvarial defect model, it showed significantly higher new bone formation than uncovered control in histomorphometric analysis. The silk membrane was covered by thin fibrotic tissue and there was not observed any inflammatory cells infiltration. In conclusion, silk fibroin membrane could be useful materials for guided bone regeneration.
Immature Development, Longevity and Fecundity of the Larval Parasitoid, Meteorus pulchricornis (Wesmael) (Hymenoptera: Braconidae), on Tobacco Cutworm
Hwang, Seok-Jo ; Byeon, Young-Woong ; Lee, Seol-Mae ; Kim, Jeong-Hwan ; Choi, Man-Young ; Kim, Sung-Hyun ; Kim, Nam-Jeong ; Park, Hae-Chul ; Lee, Young-Bo ; Lee, Sang-Beom ; Lee, Jong-Wook ;
International Journal of Industrial Entomology, volume 21, issue 2, 2010, Pages 180~183
This study was performed to investigate the temperature-dependent development, longevity and oviposition of an indigenous larval parasitoid, Meteorus pulchricornis, on tobacco cutworm, Spodoptera litura. M. pulchricornis were reared at nine constant temperatures between 15 and
. The developmental times of each three developmental stage decreased from 38.7 to 16.3 d between 15 and
. However, M. pulchricornis showed longer developmental time at
(9.5, 7.7 and 17.2 days for each three developmental stage) than at
(8.9, 7.3 and 16.3 days for each three developmental stage). Immature M. pulchricornis could not develop any more at
. The lower developmental threshold estimated by linear regression equation for the egg to cocoon, cocoon to adult emergence and egg to adult emergence were 5.1, 4.6 and
. The thermal constant for each of the three stages were 217.2, 176.2 and 403.8 degree-days, respectively. When no food or 50% honey solution as a food source is provided for M. pulchricornis, the parasitoid survived for 8.3 and 55.9 days at
, respectively. M. pulchricornis females laid 5.2 eggs daily and total of 131.6 eggs at
until it died. Peak age-specific fecundity was observed on
day (9.6 cocoons) after parasitoid emergence and gradually decreased thereafter.
The Larval Age and Mouth Morphology of the Black Soldier Fly, Hermetia illucens (Diptera: Stratiomyidae)
Kim, Won-Tae ; Bae, Sung-Woo ; Park, Hae-Cheol ; Park, Kwan-Ho ; Lee, Sang-Beom ; Choi, Young-Cheol ; Han, Sang-Mi ; Koh, Young-Ho ;
International Journal of Industrial Entomology, volume 21, issue 2, 2010, Pages 185~187
Most studies of the black soldier fly, Hermitia illucens, widely distributed throughout Southeast Asia and America focused on the use of larvae for recycling various organic materials and feed for fish and livestock. To recognize the instars of the soldier fly's larvae, we examined the number of exuviae originated from each larva from 1st instar to pupa. The weight and the head capsule width of the black soldier fly larvae also confirmed that the black soldier fly was passed through 6th instars. In addition, we found that the black soldier fly larvae had a well developed mandibular-maxillary complex, similar to those reported from the larvae of scavengers including Odontomyia cincta, Neopachygaster maculicornis, Ptecticus brunettii and Stratiomys ruficornis.
Seasonal Pupation, Adult Emergence and Mating of Black Soldier Fly, Hermetia illucens (Diptera: Stratiomyidae) in Artificial Rearing System
Park, Kwan-Ho ; Kim, Won-Tae ; Lee, Sang-Beom ; Choi, Young-Cheol ; Nho, Si-Kab ;
International Journal of Industrial Entomology, volume 21, issue 2, 2010, Pages 189~191
The black soldier fly, Hermetia illucens, has a worldwide distribution in the tropics and warm temperate regions and is active in the Korea from May through October. This species colonize a wide variety of decomposing vegetable and animal matter and oviposits in a variety of decomposing materials. In this study, how the black soldier fly pupation, adult emergence and mating rate changed with season at the artificial rearing system was investigated. The black soldier fly larvae and pupae were reared under laboratory condition (
, 60% R.H.). In this study, under the laboratory condition, pupation and adult emergence were not influenced by season, but the data shows definitely different mating numbers throughout whole year. The time of the day is changed with seasons and it influence to mating on artificial rearing. To culture the black soldier fly throughout the year in Korea needs a more deep study under the artificial rearing system.
A Newly Recorded Genus and Species in Korea, Malthacus angusticollis (Motschulsky) (Cantharidae, Coleoptera)
Kang, Tae-Hwa ; Lee, Young-Bo ; Hwang, Seok-Jo ; Park, Hae-Chul ;
International Journal of Industrial Entomology, volume 21, issue 2, 2010, Pages 193~195
Both the genus Malthacus Kirby and M. angusticollis (Motschulsky) are reported for the first time in Korea. Five North Korean specimens of M. angusticollis were loaned from Hungarian Natural History Museum, Budapest, Hungary and Sungshin Women's University, Seoul, Korea. Here we provide a key to the genus of the Podabrini in Korea and a diagnosis and taxonomic account of the genus Malthacus and M. angusticollis.