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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
International Journal of Industrial Entomology
Journal Basic Information
Journal DOI :
Korean Society of Sericultural Science
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Volume & Issues
Volume 25, Issue 2 - Dec 2012
Volume 25, Issue 1 - Sep 2012
Volume 24, Issue 2 - Jun 2012
Volume 24, Issue 1 - Mar 2012
Selecting the target year
Degumming Characteristics and Color Stability of GoldenSilk Cocoon
Kweon, Hae-Yong ; Lee, Kwang-Gill ; Park, Kwang-Young ; Kang, Seok-Woo ; Seok, Young-Seek ;
International Journal of Industrial Entomology, volume 24, issue 1, 2012, Pages 1~5
DOI : 10.7852/ijie.2012.24.1.001
A newly developed silkworm variety, GoldenSilk makes bright yellow cocoon. In this article, the basic characteristics of GoldenSilk cocoon were examined through color analysis, degumming characteristics and UV spectrophotometer. The colorant of GoldenSilk cocoon was not distributed evenly throughout the cocoon layer but existed in the outer layer of the cocoon. The colorant has not good resistance against sunlight. The degumming loss percentage of GoldenSilk cocoon was 29.2%, which is relatively higher than that of the authorized one. UV spectrophotometer showed specific absorption band around 400 to 500 nm, which is the specific yellow colorant of the GoldenSilk cocoon extracted to the degumming solution during the degumming process.
Studies on the Manifestation of Hybrid vigour and Combining Ability in Polyvoltine X Bivoltine (cross breed) Hybrids of Mulberry Silkworm Bombyx mori L.
Seshagiri, S.V. ; Naik, S. Sankar ; Seetharamulu, J. ; Goel, A.K. ; Raju, P.J. ;
International Journal of Industrial Entomology, volume 24, issue 1, 2012, Pages 7~16
DOI : 10.7852/ijie.2012.24.1.007
To meet the local requirement i.e. the tropical stress of Andhra Pradesh and marginal farmers, twenty four new hybrid combinations were tested in Line x Tester method involving eight newly developed polyvoltine oval lines viz., MSO1, MSO2, MSO3, MSO4, MSO5, MSO6, MSO7 and MSO8 and three bivoltine silkworm breeds viz., APS12, APS45 and APS8 as testers. These hybrids were reared to evaluate their performance. Data for eight economically important metric traits viz., fecundity, cocoon yield per 10,000 larvae by number, cocoon yield per 10,000 larvae by weight, survival rate, cocoon weight, cocoon shell weight, cocoon shell ratio and filament length was recorded. General combining ability (gca), specific combining ability (sca) and heterosis were analyzed. Further, the relative merit of the hybrids for all the traits under study was also assessed adopting Evaluation Index method. Based on combining ability effects, heterosis and evaluation index, five hybrid combinations viz., MSO3
APS45 and MSO1
APS45 were selected for further evaluation in different seasons.
Cocoon Filament Quality of a Special Silkworm Variety, GoldenSilk
Kweon, Hae-Yong ; Lee, Kwang-Gill ; Park, Kwang-Young ; Kang, Seok-Woo ; Kang, Pil-Don ; Kim, Mi-Ja ; Kim, Kee-Young ;
International Journal of Industrial Entomology, volume 24, issue 1, 2012, Pages 17~21
DOI : 10.7852/ijie.2012.24.1.017
The effect of cultivation season on the cocoon filament quality of authorized silkworm variety, GoldenSilk was examined with cocoon filament character, reeling character, and fiber character during 3 years. In cocoon filament character, filament length of spring and autumn cocoon was 979 and 879 m, respectively. Cocoon filament weight of spring and autumn cocoon was 33.1 and 26.5 cg, which is lower than that of general bivoltine strain, BaeGokJam. Raw silk percentage of spring and autumn cocoon was 15.65 and 13.77%, respectively. In reeling character, reelability showed similar trend regardless of cultivation season and strain. However, the length and weight of non-broken filament of spring season were slightly higher than those of autumn cocoon. Cultivation season did not affect on the fiber characteristics such as clearness and neatness, lousiness, and degumming loss. In cocoon character, filament length, size, and weight, spring cocoon filament showed higher value than autumn cocoon, but lower than authorized general silkworm varieties. In reeling characteristics, non-broken length and weight showed the similar trends with the cocoon filament character. However, the reelability of GoldenSilk cocoon was not affected by cultivation season. The reelability of GoldenSilk cocoon was also similar with that of authorized general one. Fiber character including clearness and neatness, lousiness, and degumming loss was also similar regardless of the rearing season and different strain.
Efficient Production of Porcine Circovirus Type 2 Capsid Protein using Baculovirus
Lee, Jun-Beom ; Bae, Sung-Min ; Kim, Hee-Jung ; Lee, Won-Woo ; Heo, Won-Il ; Shin, Tae-Young ; Choi, Jae-Bang ; Woo, Soo-Dong ;
International Journal of Industrial Entomology, volume 24, issue 1, 2012, Pages 23~27
DOI : 10.7852/ijie.2012.24.1.023
Porcine circovirus type 2 (PCV2) is a single-stranded circular DNA virus associated with Postweaning multisystemic wasting syndrome (PMWS), which is considered to be an important infectious swine viral disease. PCV2 capsid protein encoded by ORF2 is a structural protein and expected as the high immunogenicity protein. In this study, we generated recombinant baculovirus containing ORF2 of PCV2 and analyzed the optimal conditions for the production of capsid protein in insect cell. Production and status of recombinant capsid protein in insect cell were confirmed by SDS-PAGE and Western blot analysis using His tag antibody and anti-PCV2 serum. The yield of recombinant capsid protein was high like as shown visible on SDS-PAGE. Optimal multiplicity of infection (MOI) and infection time of recombinant virus were determined as 5 MOI and 4 days, respectively. ORF2 is known to have N-linked glycosylation site, but we couldn't detect the glycosylation of recombinant protein in insect cells.
Availability of Sugar Solutions for Colony Development and Progeny-Queen Production of the European Bumblebee, Bombus terrestris
Yoon, Hyung-Joo ; Lee, Kyeong-Yong ; Kim, Mi-Ae ; Han, Sang-Mi ; Ahn, Mi-Young ; Park, In-Gyun ;
International Journal of Industrial Entomology, volume 24, issue 1, 2012, Pages 29~35
DOI : 10.7852/ijie.2012.24.1.029
Bumblebees are widely used to pollinate various crops, especially tomato, in greenhouses and fields. Here, we investigated whether different sugar solutions have any effects on the oviposition and colony development of Bombus terrestris queens. The types of sugar solution used were a white sugar solution, brown sugar solution, dark brown sugar solution, and prepared sugar solution; all solution contained with a 40% sugar concentration and 0.3% sorbic acid. Among these sugar solutions, B. terrestris queens reared on the white sugar solution exhibited the best results; the rates of oviposition, colony foundation and progeny-queen production were
, respectively, which corresponded to 1.1-33.0-fold increases over the results of other sugar solutions. Queens reared on the prepared sugar solution did not found any colonies. The death rate within one month was lowest for the queens reared on the white sugar solution at
, which was 1.2-2.0-fold lower than the rate for the other sugar solutions. Furthermore, the numbers of progeny produced by queens reared on the white sugar solution,
queens, were also higher, corresponding to 1.1-1.2-fold increases compared to queens raised on the other sugar solutions. Therefore, the white sugar solution was the favorable sugar solution for the egg-laying and colony developmental characteristics of B. terrestris queens.
Expression and Antibacterial Activity of a Bombus ignitus Apidaecin in Baculovirus-Infected Insect Cells
Lee, Kwang-Sik ; Je, Yeon-Ho ; Jin, Byung-Rae ; Sohn, Hung-Dae ;
International Journal of Industrial Entomology, volume 24, issue 1, 2012, Pages 37~40
DOI : 10.7852/ijie.2012.24.1.037
The apidaecins are highly active against Gram-negative bacteria. Here, we show the expression and antibacterial activity of the bumblebee, Bombus ignitus, apidaecin. We PCR-amplified 51 bp of the active domain sequence of the B. ignitus apidaecin gene and expressed the recombinant B. ignitus apidaecin active domain in baculovirus-infected insect cells. The recombinant B. ignitus apidaecin active domain shows bactericidal activity against Gram-negative bacteria, including Pseudomonas tolaasii, a serious pathogen in cultivated mushrooms, but not Gram-positive bacteria. This result suggests that the active domain of the B. ignitus apidaecin is a potential antibacterial agent for the control of bacterial brown blotch diseases.
COII Sequence-based Study for Population Genetic Variation of a Ground Beetle, Scarites aterrimus (Coleoptera : Carabidae)
Wang, Ah-Rha ; Kim, Ik-Soo ;
International Journal of Industrial Entomology, volume 24, issue 1, 2012, Pages 41~47
DOI : 10.7852/ijie.2012.24.1.041
The Scarites aterrimus (Coleoptera: Carabidae) dwells exclusively on coastal sandy dunes. Previously, we investigated the nation-wide magnitude and nature of genetic diversity of the species using mitochondrial COI gene and found moderate to low magnitude of sequence diversity, the presence of closely related haplotypes, and relatively high gene flow estimate. Based on these observations we concluded that the species had no historical barriers that bolster genetic subdivision and possible population decline. In this study, we additionally sequenced mitochondrial COII gene from 23 individuals collected from 9 Korean localities to confirm previous findings. Sequencing of 688 bp COII gene provided 5 haplotypes ranging in sequence divergence from 0.145% to 0.291% (1 ~ 2 bp), further confirming low sequence divergence of the species. Gene flow estimates and genetic diversity estimates also support the previous findings that there had been no historical barriers that bolster genetic subdivision.